AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99854


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99854.t1 MTR_2g105480 100.000 66 0 0 1 66 353 418 1.73e-40 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99854.t1 AT1G73110 63.636 66 24 0 1 66 270 335 1.16e-24 93.6
MS.gene99854.t1 AT1G73110 63.636 66 24 0 1 66 367 432 2.99e-24 93.6

Find 10 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTAGTGGGTTGATGATAT+TGG 0.294945 2.1:+390695 None:intergenic
CAAAGAACAACAGTTGATAA+TGG 0.386217 2.1:+392105 MS.gene99854:CDS
GAATAAATACAGGAAGATTC+TGG 0.403777 2.1:-390763 None:intergenic
ATCGATGCTTTACTTGAGTC+AGG 0.418408 2.1:+392070 MS.gene99854:CDS
ACCTCTGGAGGAATAAATAC+AGG 0.480480 2.1:-390773 None:intergenic
CAAATTTCTCAAAAGAAGAA+AGG 0.512041 2.1:+390739 MS.gene99854:CDS
TCCTGTATTTATTCCTCCAG+AGG 0.540010 2.1:+390772 MS.gene99854:CDS
AATGGAAACTAAACTTTCGA+AGG 0.571792 2.1:+392123 MS.gene99854:CDS
GGATGACTATTTACCTCTGG+AGG 0.584674 2.1:-390785 None:intergenic
TAGTGGGTTGATGATATTGG+AGG 0.612663 2.1:+390698 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTCATCAATATTAATT+TGG - chr2.1:390881-390900 None:intergenic 10.0%
!!! ATTTGATTGTATTTTAAAAA+AGG + chr2.1:391027-391046 MS.gene99854:intron 10.0%
!! AAATATAGCCAAAATTATTA+GGG + chr2.1:390961-390980 MS.gene99854:intron 15.0%
!! ATAAATAAGATTGAAATCTA+AGG + chr2.1:391303-391322 MS.gene99854:intron 15.0%
!! TAAATAAGATTGAAATCTAA+GGG + chr2.1:391304-391323 MS.gene99854:intron 15.0%
!! TAAATATAGCCAAAATTATT+AGG + chr2.1:390960-390979 MS.gene99854:intron 15.0%
!! ATAAATAAGACTGAAATCTA+AGG - chr2.1:391118-391137 None:intergenic 20.0%
!! TAAATAAGACTGAAATCTAA+GGG - chr2.1:391117-391136 None:intergenic 20.0%
!! TATTGAAACTCAAAATTTGA+AGG + chr2.1:391634-391653 MS.gene99854:intron 20.0%
!! TTATTGATATTATCATGCAA+AGG + chr2.1:391491-391510 MS.gene99854:intron 20.0%
!!! AAATTAACTGCTTTTCTATT+TGG + chr2.1:392033-392052 MS.gene99854:intron 20.0%
!!! AAATTAGTTGCAAATGTTTT+GGG + chr2.1:391876-391895 MS.gene99854:intron 20.0%
!!! TTATTGGCTAACATTAATTT+CGG + chr2.1:391437-391456 MS.gene99854:intron 20.0%
!!! TTATTGGCTAACATTAATTT+CGG - chr2.1:391456-391437 None:intergenic 20.0%
! AAAATGCTAGATATCAAGAA+TGG + chr2.1:391050-391069 MS.gene99854:intron 25.0%
! ATAACGATAAAAACATCACA+AGG - chr2.1:391701-391720 None:intergenic 25.0%
! ATCACAAGGTAAAATATCAT+GGG - chr2.1:391687-391706 None:intergenic 25.0%
! CAAATTTCTCAAAAGAAGAA+AGG + chr2.1:390739-390758 MS.gene99854:CDS 25.0%
!! AATGTTACTCTTTAAAGACA+AGG - chr2.1:391972-391991 None:intergenic 25.0%
!! AATTAGTTGCAAATGTTTTG+GGG + chr2.1:391877-391896 MS.gene99854:intron 25.0%
!! GAAATTAGTTGCAAATGTTT+TGG + chr2.1:391875-391894 MS.gene99854:intron 25.0%
!! TATTGGCTAACATTAATTTC+GGG - chr2.1:391457-391438 None:intergenic 25.0%
!! TCAATATTAATTTGGTTGAC+AGG - chr2.1:390873-390892 None:intergenic 25.0%
!!! CTTTCTTCTTTTGAGAAATT+TGG - chr2.1:390741-390760 None:intergenic 25.0%
AATGGAAACTAAACTTTCGA+AGG + chr2.1:392123-392142 MS.gene99854:CDS 30.0%
CATCACAAGGTAAAATATCA+TGG - chr2.1:391688-391707 None:intergenic 30.0%
GAATAAATACAGGAAGATTC+TGG - chr2.1:390766-390785 None:intergenic 30.0%
TCTTGATATATAGCATTGCT+CGG - chr2.1:391174-391193 None:intergenic 30.0%
TGAAGAACATTGAAGACTAA+TGG + chr2.1:392152-392171 MS.gene99854:CDS 30.0%
TTCATCTTCTATCTATCCAA+TGG - chr2.1:391534-391553 None:intergenic 30.0%
! CTACGTTTGTAATTTTCCAT+TGG + chr2.1:391515-391534 MS.gene99854:intron 30.0%
! CTTTAAAGACAAGGAAAAGA+AGG - chr2.1:391963-391982 None:intergenic 30.0%
!! ATTAGTTGCAAATGTTTTGG+GGG + chr2.1:391878-391897 MS.gene99854:intron 30.0%
!! CAAAGAACAACAGTTGATAA+TGG + chr2.1:392105-392124 MS.gene99854:CDS 30.0%
!! TAACTGCTTTTCTATTTGGT+TGG + chr2.1:392037-392056 MS.gene99854:intron 30.0%
!! TCAAGAATGGATTTTCTTGA+TGG + chr2.1:391063-391082 MS.gene99854:intron 30.0%
AAAAGAATTGGCATGAGAGT+CGG - chr2.1:391766-391785 None:intergenic 35.0%
AAAGGATGACTATTTACCTC+TGG - chr2.1:390791-390810 None:intergenic 35.0%
ACAAGGTAAAATATCATGGG+TGG - chr2.1:391684-391703 None:intergenic 35.0%
AGCCAAAATTATTAGGGTCA+TGG + chr2.1:390967-390986 MS.gene99854:intron 35.0%
! GACCATGACCCTAATAATTT+TGG - chr2.1:390972-390991 None:intergenic 35.0%
!! TGATGGATTCAAGAATGACA+TGG + chr2.1:391080-391099 MS.gene99854:intron 35.0%
ATCGATGCTTTACTTGAGTC+AGG + chr2.1:392070-392089 MS.gene99854:CDS 40.0%
ATCGTTATCTCTCTTGCACT+TGG + chr2.1:391713-391732 MS.gene99854:intron 40.0%
ATGTGTCTAACATAGCAGAC+AGG - chr2.1:391809-391828 None:intergenic 40.0%
ATGTTGGCCAGCAAAAGAAT+TGG - chr2.1:391778-391797 None:intergenic 40.0%
CAGAACAGAACAGGAGAAAA+AGG - chr2.1:390809-390828 None:intergenic 40.0%
GATATATAGCATTGCTCGGT+GGG - chr2.1:391170-391189 None:intergenic 40.0%
GTAGGCTAACTGATGGTAAT+TGG + chr2.1:390841-390860 MS.gene99854:intron 40.0%
TAGCCAATAAAATCTCAGCC+TGG + chr2.1:391229-391248 MS.gene99854:intron 40.0%
TAGCCAATAAAATCTCAGCC+TGG - chr2.1:391248-391229 None:intergenic 40.0%
TCCTGTATTTATTCCTCCAG+AGG + chr2.1:390772-390791 MS.gene99854:CDS 40.0%
TGATATATAGCATTGCTCGG+TGG - chr2.1:391171-391190 None:intergenic 40.0%
! ACCTCTGGAGGAATAAATAC+AGG - chr2.1:390776-390795 None:intergenic 40.0%
! TCTCATGCCAATTCTTTTGC+TGG + chr2.1:391768-391787 MS.gene99854:intron 40.0%
! TCTTTTGCTGGCCAACATTT+GGG + chr2.1:391780-391799 MS.gene99854:intron 40.0%
! TTCTTTTGCTGGCCAACATT+TGG + chr2.1:391779-391798 MS.gene99854:intron 40.0%
ACAACAGGACAGAACAGAAC+AGG - chr2.1:390818-390837 None:intergenic 45.0%
CAATAAAATCTCAGCCTGGC+CGG + chr2.1:391233-391252 MS.gene99854:intron 45.0%
CAGACAGGATACCCAAATGT+TGG - chr2.1:391794-391813 None:intergenic 45.0%
GGATGACTATTTACCTCTGG+AGG - chr2.1:390788-390807 None:intergenic 45.0%
TCAGCCTGGCTGAAAATCAT+GGG - chr2.1:391413-391432 None:intergenic 45.0%
TTCTGTTCTGTCCTGTTGTG+TGG + chr2.1:390819-390838 MS.gene99854:intron 45.0%
TTGTGTGGTAGGCTAACTGA+TGG + chr2.1:390834-390853 MS.gene99854:intron 45.0%
! CAGCCAGGCTGAGATTTTAT+TGG + chr2.1:391421-391440 MS.gene99854:intron 45.0%
!!! AGTTGCAAATGTTTTGGGGG+AGG + chr2.1:391881-391900 MS.gene99854:intron 45.0%
CAGCCTGGCTGAAAATCATG+GGG - chr2.1:391412-391431 None:intergenic 50.0%
CTCAGCCTGGCTGAAAATCA+TGG - chr2.1:391414-391433 None:intergenic 50.0%
GCTGAAAATCATGGGGTGGT+GGG - chr2.1:391405-391424 None:intergenic 50.0%
GTTCTGTCCTGTTGTGTGGT+AGG + chr2.1:390823-390842 MS.gene99854:intron 50.0%
! CAGTTAGCCTACCACACAAC+AGG - chr2.1:390833-390852 None:intergenic 50.0%
! CGGCCAGGCTGAGATTTTAT+TGG - chr2.1:391235-391254 None:intergenic 50.0%
!! AAATCATGGGGTGGTGGGTA+CGG - chr2.1:391400-391419 None:intergenic 50.0%
ACCCACCACCCCATGATTTC+CGG - chr2.1:391255-391274 None:intergenic 55.0%
CCTGGCTGAAAATCATGGGG+TGG - chr2.1:391409-391428 None:intergenic 55.0%
GGCTGAAAATCATGGGGTGG+TGG - chr2.1:391406-391425 None:intergenic 55.0%
TCAGCCTGGCCGGAAATCAT+GGG + chr2.1:391243-391262 MS.gene99854:intron 55.0%
! CCACCCCATGATTTTCAGCC+AGG + chr2.1:391406-391425 MS.gene99854:intron 55.0%
CAGCCTGGCCGGAAATCATG+GGG + chr2.1:391244-391263 MS.gene99854:intron 60.0%
CTCAGCCTGGCCGGAAATCA+TGG + chr2.1:391242-391261 MS.gene99854:intron 60.0%
GCCGGAAATCATGGGGTGGT+GGG + chr2.1:391251-391270 MS.gene99854:intron 60.0%
!! TGGGGTGGTGGGTACGGAAA+AGG - chr2.1:391394-391413 None:intergenic 60.0%
CCACCCCATGATTTCCGGCC+AGG - chr2.1:391250-391269 None:intergenic 65.0%
CCTGGCCGGAAATCATGGGG+TGG + chr2.1:391247-391266 MS.gene99854:intron 65.0%
GGCCGGAAATCATGGGGTGG+TGG + chr2.1:391250-391269 MS.gene99854:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 390701 392171 390701 ID=MS.gene99854
chr2.1 mRNA 390701 392171 390701 ID=MS.gene99854.t1;Parent=MS.gene99854
chr2.1 exon 390701 390793 390701 ID=MS.gene99854.t1.exon1;Parent=MS.gene99854.t1
chr2.1 CDS 390701 390793 390701 ID=cds.MS.gene99854.t1;Parent=MS.gene99854.t1
chr2.1 exon 392064 392171 392064 ID=MS.gene99854.t1.exon2;Parent=MS.gene99854.t1
chr2.1 CDS 392064 392171 392064 ID=cds.MS.gene99854.t1;Parent=MS.gene99854.t1
Gene Sequence

>MS.gene99854

TGGGTTGATGATATTGGAGGTGCTGAAAGTTTTGCATCCAAATTTCTCAAAAGAAGAAAGGACCAGAATCTTCCTGTATTTATTCCTCCAGAGGTAAATAGTCATCCTTTTTCTCCTGTTCTGTTCTGTCCTGTTGTGTGGTAGGCTAACTGATGGTAATTGGCTGTTTCCTGTCAACCAAATTAATATTGATGAAATATTGATTCAGCTCATATATAAGTTGAACATAGTTTATAGACGAACATAAAATTATAAGATCTAAATATAGCCAAAATTATTAGGGTCATGGTCTTAACATTGTAGCGTTGTTCAAATCGACTTGACTGATTTGATTGTATTTTAAAAAAGGAAAATGCTAGATATCAAGAATGGATTTTCTTGATGGATTCAAGAATGACATGGCAGAATAATCACCCTTAGATTTCAGTCTTATTTATCAACGTTTCATAAAAAAAAATACATAATACCCACCGAGCAATGCTATATATCAAGAAAGATTTTATCAAGAAATTGTCCCGAAATTAATGTTAGCCAATAAAATCTCAGCCTGGCCGGAAATCATGGGGTGGTGGGTGTTATGTATTTTTTTTTATGAAACTTTAATAAATAAGATTGAAATCTAAGGGTGATTATTCTGTCATGTCATTCTTGAATTCATCAAGAAAATTCATTCTTGATATCTAGCATTTTCCTTTTCCGTACCCACCACCCCATGATTTTCAGCCAGGCTGAGATTTTATTGGCTAACATTAATTTCGGAACAATTTCTTGATAAAATTGTTATGAATAGTTATTGATATTATCATGCAAAGGTCTACGTTTGTAATTTTCCATTGGATAGATAGAAGATGAACAGTCTAGTTCTAAATTGAATTCGTCAAGATGATGTCTTAATATGTGATTTTCAACATCATAGTACGACTTGATTACAGATATTGAAACTCAAAATTTGAAGGACATTAGCTTATCTTTAAGAATTTCCACCCATGATATTTTACCTTGTGATGTTTTTATCGTTATCTCTCTTGCACTTGGAAAGAGATCTTTCGTTATAATTTCTTTCCGACTCTCATGCCAATTCTTTTGCTGGCCAACATTTGGGTATCCTGTCTGCTATGTTAGACACATAAAGTTCTTAACTCATACTTCTGATATTTGAACACTTCATTCATGAGAAATTAGTTGCAAATGTTTTGGGGGAGGAAACATTCATAGTTATATGTAATAATAGAACTTCTGCTTTTCATCTTTCTTTCTTTCCTTCTTTTCCTTGTCTTTAAAGAGTAACATTTCTATTATTGTTGATTTGCTTTACTGAAAAAAATACAAATGAAATTAACTGCTTTTCTATTTGGTTGGATAGCAAACGATCGATGCTTTACTTGAGTCAGGTTATTCTCTGCTCAAAGAACAACAGTTGATAATGGAAACTAAACTTTCGAAGGAATACATGAAGAACATTGAAGACTAA

Protein sequence

>MS.gene99854.t1

WVDDIGGAESFASKFLKRRKDQNLPVFIPPEQTIDALLESGYSLLKEQQLIMETKLSKEYMKNIED