AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55270.t1 MTR_5g041910 96.471 85 3 0 1 85 177 261 2.40e-54 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55270.t1 AT1G14150 71.765 85 24 0 1 85 106 190 1.16e-39 129
MS.gene55270.t1 AT1G14150 64.815 54 19 0 1 54 106 159 1.37e-20 80.5

Find 11 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACAGAATTATCTTGAAC+AGG 0.378711 5.2:-31419895 MS.gene55270:CDS
TGTTGGAAACTTTGTCTTGG+AGG 0.429693 5.2:-31419941 MS.gene55270:CDS
TCAATGTCTTCTCGTAGTAT+AGG 0.434954 5.2:+31418745 None:intergenic
AGGTATGATTCATATACCTT+TGG 0.468647 5.2:+31418765 None:intergenic
AAATGTTGGAAACTTTGTCT+TGG 0.489316 5.2:-31419944 MS.gene55270:CDS
TCGTTATGTGCGGACACCAA+AGG 0.589271 5.2:-31418781 MS.gene55270:CDS
GAAATCAATAGATGAACTTG+TGG 0.590899 5.2:-31418724 MS.gene55270:CDS
TGTTAAAACTGCTAATGAGT+TGG 0.596114 5.2:-31419823 MS.gene55270:CDS
TTTGCCTGAAAATCGTCGCA+AGG 0.639622 5.2:-31419850 MS.gene55270:CDS
GTTGGTTGATAATTTGACTG+AGG 0.655182 5.2:-31419805 MS.gene55270:intron
TCCAGATGGATCGTTATGTG+CGG 0.669027 5.2:-31418791 MS.gene55270:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAGATAATTTAGTCAT+TGG - chr5.2:31419157-31419176 MS.gene55270:intron 15.0%
!! TACAAGTTAATTATTAATAG+TGG - chr5.2:31419809-31419828 MS.gene55270:CDS 15.0%
!! TGAAATTGTTAATGAATATA+AGG - chr5.2:31419678-31419697 MS.gene55270:intron 15.0%
!!! TCTATTAGTTGAATTTTTTA+AGG + chr5.2:31419308-31419327 None:intergenic 15.0%
!!! TGATTTTCTCAAATTATTAA+TGG + chr5.2:31419532-31419551 None:intergenic 15.0%
!!! TGTATTTTTAGTATATGTTT+AGG - chr5.2:31419734-31419753 MS.gene55270:intron 15.0%
!!! TTATTACTATAATTCTGAAA+AGG + chr5.2:31419143-31419162 None:intergenic 15.0%
!!! AATTTATATACCTGTTTTTG+AGG + chr5.2:31419403-31419422 None:intergenic 20.0%
!!! TCAATTTCATGGATTAATTT+GGG - chr5.2:31419337-31419356 MS.gene55270:intron 20.0%
! AAAAATTGTCTGTTCTACAT+AGG - chr5.2:31419634-31419653 MS.gene55270:intron 25.0%
! AAAAGGAGAATGTTAAATAG+TGG + chr5.2:31419126-31419145 None:intergenic 25.0%
! GATTCAAATTGTCAATTTCA+TGG - chr5.2:31419326-31419345 MS.gene55270:intron 25.0%
! TGTGTTAAATTAGTTAAGCT+GGG - chr5.2:31419279-31419298 MS.gene55270:intron 25.0%
! TTCAGTGTAATTATATGTGT+CGG - chr5.2:31419557-31419576 MS.gene55270:intron 25.0%
! TTGTGTTAAATTAGTTAAGC+TGG - chr5.2:31419278-31419297 MS.gene55270:intron 25.0%
!! GTCAATTTCATGGATTAATT+TGG - chr5.2:31419336-31419355 MS.gene55270:intron 25.0%
!! TATGTAGAACAGACAATTTT+TGG + chr5.2:31419635-31419654 None:intergenic 25.0%
!!! AGTCTATGAACATGTTTTTT+CGG - chr5.2:31418944-31418963 MS.gene55270:intron 25.0%
!!! TAGTTTTAATCTATGAAGCA+TGG - chr5.2:31419473-31419492 MS.gene55270:intron 25.0%
AATATAAGGTGCAATACAAG+AGG - chr5.2:31419692-31419711 MS.gene55270:intron 30.0%
AATTAGCTATCCTCAAAAAC+AGG - chr5.2:31419390-31419409 MS.gene55270:intron 30.0%
AGGTATGATTCATATACCTT+TGG + chr5.2:31419890-31419909 None:intergenic 30.0%
GAAACAGAATTATCTTGAAC+AGG - chr5.2:31418757-31418776 MS.gene55270:CDS 30.0%
GAAATCAATAGATGAACTTG+TGG - chr5.2:31419928-31419947 MS.gene55270:CDS 30.0%
TGACAAAATAGAGTTCAGAT+TGG + chr5.2:31419442-31419461 None:intergenic 30.0%
TGTTAAAACTGCTAATGAGT+TGG - chr5.2:31418829-31418848 MS.gene55270:intron 30.0%
! AGTATATGTTTAGGTTCGAT+TGG - chr5.2:31419743-31419762 MS.gene55270:intron 30.0%
!! GAACAGACAATTTTTGGATT+AGG + chr5.2:31419629-31419648 None:intergenic 30.0%
!! TATGCTTAATTTGTTGCTTG+TGG - chr5.2:31418874-31418893 MS.gene55270:intron 30.0%
!!! TATGAACATGTTTTTTCGGA+GGG - chr5.2:31418948-31418967 MS.gene55270:intron 30.0%
TAAATAGTGGTCTCAGACAT+TGG + chr5.2:31419113-31419132 None:intergenic 35.0%
TCAATGTCTTCTCGTAGTAT+AGG + chr5.2:31419910-31419929 None:intergenic 35.0%
!! GTTGGTTGATAATTTGACTG+AGG - chr5.2:31418847-31418866 MS.gene55270:intron 35.0%
!!! CTATGAACATGTTTTTTCGG+AGG - chr5.2:31418947-31418966 MS.gene55270:intron 35.0%
TGTTGGAAACTTTGTCTTGG+AGG - chr5.2:31418711-31418730 MS.gene55270:CDS 40.0%
TTCGTCCAAATTAGCTCATC+TGG - chr5.2:31419222-31419241 MS.gene55270:intron 40.0%
TTGAGCCAGATGAGCTAATT+TGG + chr5.2:31419230-31419249 None:intergenic 40.0%
! ATATTTTTCCCCTCTCCAGA+TGG - chr5.2:31419847-31419866 MS.gene55270:CDS 40.0%
AACCAGTGTCATCACTGCAA+TGG - chr5.2:31418978-31418997 MS.gene55270:intron 45.0%
AACCATTGCAGTGATGACAC+TGG + chr5.2:31418983-31419002 None:intergenic 45.0%
ACATAACGATCCATCTGGAG+AGG + chr5.2:31419860-31419879 None:intergenic 45.0%
ATAACGATCCATCTGGAGAG+GGG + chr5.2:31419858-31419877 None:intergenic 45.0%
CATAACGATCCATCTGGAGA+GGG + chr5.2:31419859-31419878 None:intergenic 45.0%
TCCAGATGGATCGTTATGTG+CGG - chr5.2:31419861-31419880 MS.gene55270:CDS 45.0%
TTTGCCTGAAAATCGTCGCA+AGG - chr5.2:31418802-31418821 MS.gene55270:intron 45.0%
! TACTCCTTGCGACGATTTTC+AGG + chr5.2:31418809-31418828 None:intergenic 45.0%
ACACGACATGTGTCCGACAT+CGG - chr5.2:31419597-31419616 MS.gene55270:intron 50.0%
GATTAGGCGTGTTCCGATGT+CGG + chr5.2:31419613-31419632 None:intergenic 50.0%
TCCGCACATAACGATCCATC+TGG + chr5.2:31419865-31419884 None:intergenic 50.0%
TCGTTATGTGCGGACACCAA+AGG - chr5.2:31419871-31419890 MS.gene55270:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 31418710 31419964 31418710 ID=MS.gene55270
chr5.2 mRNA 31418710 31419964 31418710 ID=MS.gene55270.t1;Parent=MS.gene55270
chr5.2 exon 31419806 31419964 31419806 ID=MS.gene55270.t1.exon1;Parent=MS.gene55270.t1
chr5.2 CDS 31419806 31419964 31419806 ID=cds.MS.gene55270.t1;Parent=MS.gene55270.t1
chr5.2 exon 31418710 31418808 31418710 ID=MS.gene55270.t1.exon2;Parent=MS.gene55270.t1
chr5.2 CDS 31418710 31418808 31418710 ID=cds.MS.gene55270.t1;Parent=MS.gene55270.t1
Gene Sequence

>MS.gene55270

ATGTTGGAAACTTTGTCTTGGAGGTATGTTATGTTTTATATAAGATTGAAACAGAATTATCTTGAACAGGATTTGAAAAATGCTATCGCTACTTTGCCTGAAAATCGTCGCAAGGAGTATGTTAAAACTGCTAATGAGTTGGTTGATAATTTGACTGAGGTATGTATGCTTAATTTGTTGCTTGTGGATTAAATTTTAGTGCTTGTTTTGTTATTTTGATTAATGAATTGAATTAGTCTATGAACATGTTTTTTCGGAGGGTATTGCTAACCAGTGTCATCACTGCAATGGTTAAGAAATCTATAAATAGAAAATTTATCTTGAAAACATAAGTTTTGACTTTTGATTAAGTAATAATTACACTATTTTTGATAAAAAGTTACTTGTTTTAGTTGCTTAACCAATGTCTGAGACCACTATTTAACATTCTCCTTTTCAGAATTATAGTAATAAGATAATTTAGTCATTGGAATTAATGAAATTCGAAAATATTTTGAAAATTAAAATTTTATTTCGTCCAAATTAGCTCATCTGGCTCAAGTTGTCAAGTTTATGATTTATTTTGAATTTGTGTTAAATTAGTTAAGCTGGGTTACCTTAAAAAATTCAACTAATAGATTCAAATTGTCAATTTCATGGATTAATTTGGGTGATGAAATATGAGTTTGACATGTTGTGTTAATTAGCTATCCTCAAAAACAGGTATATAAATTAGTTTTACACTATCATCCAATCTGAACTCTATTTTGTCATGTGATATCATTAGTTTTAATCTATGAAGCATGGATACAGACACAACACAGATATGACACGCATATACCATTAATAATTTGAGAAAATCATATAATTCAGTGTAATTATATGTGTCGGTGTTGTATCGATGTTGAACACGACATGTGTCCGACATCGGAACACGCCTAATCCAAAAATTGTCTGTTCTACATAGGTTTTAATTATATAATTATAGATGAAATTGTTAATGAATATAAGGTGCAATACAAGAGGTATTGTAGTGATCATTATCTGTATTTTTAGTATATGTTTAGGTTCGATTGGCAAAATAAGAGAACTTAACATCTAATTATAGCTTAACGTACAATACAAGTTAATTATTAATAGTGGTCTTATAATGTGATTATATTTTTCCCCTCTCCAGATGGATCGTTATGTGCGGACACCAAAGGTATATGAATCATACCTATACTACGAGAAGACATTGAAATCAATAGATGAACTTGTGGCTCTGTTAGCATAG

Protein sequence

>MS.gene55270.t1

MLETLSWRYVMFYIRLKQNYLEQDLKNAIATLPENRRKEYVKTANELVDNLTEMDRYVRTPKVYESYLYYEKTLKSIDELVALLA