AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55349


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55349.t1 MTR_5g043580 97.727 176 4 0 1 176 1 176 6.06e-128 356
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55349.t1 AT2G03420 73.585 106 28 0 69 174 63 168 1.02e-53 168

Find 37 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTACGAGGGGCGGAAT+GGG 0.329826 5.2:+33114728 MS.gene55349:CDS
CTTCTATTGCAATTCTTCTC+TGG 0.348974 5.2:+33113344 MS.gene55349:CDS
ATGAATTTGTCCCTTGCCTC+TGG 0.354711 5.2:-33114783 None:intergenic
CCTCAAAAGAAGGTTCAGTT+TGG 0.387035 5.2:+33113235 MS.gene55349:CDS
AAGTCAATCTGAGGCAGCTC+AGG 0.441780 5.2:-33113850 None:intergenic
TGAAACCATTAAAACGTTTG+AGG 0.455321 5.2:-33113873 None:intergenic
ACAGGACTAGAGTCTGTTCC+TGG 0.456712 5.2:+33113826 MS.gene55349:CDS
GTAAGAATGATGAGAAGATA+AGG 0.480601 5.2:-33113290 None:intergenic
CGAGGGGCGGAATGGGGAGT+TGG 0.490686 5.2:+33114735 MS.gene55349:CDS
GCTCAAGCAGTTTCTTCACC+AGG 0.491458 5.2:-33114041 None:intergenic
AGATAAGGAGCAAGAAAACT+TGG 0.492619 5.2:-33113275 None:intergenic
ACTGCTTACGAGGGGCGGAA+TGG 0.499290 5.2:+33114727 MS.gene55349:CDS
CATAGCAATGTTGAAGGAGA+AGG 0.511435 5.2:+33114803 MS.gene55349:CDS
GCACATGCTGGATTCATGTC+AGG 0.519014 5.2:+33113799 MS.gene55349:intron
ATCTGAGGCAGCTCAGGACC+AGG 0.523597 5.2:-33113844 None:intergenic
CAAATTCATAGCAATGTTGA+AGG 0.527626 5.2:+33114797 MS.gene55349:CDS
ACTCAGCAATCCTCAAAAGA+AGG 0.529490 5.2:+33113225 MS.gene55349:CDS
ATTCTTCTCTGGTCAACTCC+AGG 0.534134 5.2:+33113355 MS.gene55349:CDS
CTCAAGCAGTTTCTTCACCA+GGG 0.535692 5.2:-33114040 None:intergenic
GTGGAAATCTGAATGAAAGA+TGG 0.538239 5.2:-33113190 None:intergenic
GGGAATGACTCCAGAGGCAA+GGG 0.550474 5.2:+33114773 MS.gene55349:CDS
GGATTCATGTCAGGAATCAC+AGG 0.551319 5.2:+33113808 MS.gene55349:CDS
TCAAGCAGTTTCTTCACCAG+GGG 0.567433 5.2:-33114039 None:intergenic
TGCTTACGAGGGGCGGAATG+GGG 0.568619 5.2:+33114729 MS.gene55349:CDS
AATGAAAGATGGTAATGAAG+AGG 0.576236 5.2:-33113179 None:intergenic
AGGGAATGACTCCAGAGGCA+AGG 0.577514 5.2:+33114772 MS.gene55349:CDS
GAGAACAAGTACTGCTTACG+AGG 0.584541 5.2:+33114717 MS.gene55349:CDS
GTTGGTGATTGTTCAGCAGA+GGG 0.594398 5.2:+33114753 MS.gene55349:CDS
AGTTGGTGATTGTTCAGCAG+AGG 0.606424 5.2:+33114752 MS.gene55349:CDS
CAAGTACTGCTTACGAGGGG+CGG 0.612792 5.2:+33114722 MS.gene55349:CDS
GCAATGTTGAAGGAGAAGGT+TGG 0.630694 5.2:+33114807 MS.gene55349:CDS
CGTTTGAGGAAGTCAATCTG+AGG 0.656409 5.2:-33113859 None:intergenic
AGAACAAGTACTGCTTACGA+GGG 0.671236 5.2:+33114718 MS.gene55349:CDS
ATTGCAATAGAAGCAGCCAA+AGG 0.674848 5.2:-33113334 None:intergenic
AAGATTTAAAGAGACACCCC+TGG 0.680049 5.2:+33114023 MS.gene55349:CDS
AGCAGAGGGAATGACTCCAG+AGG 0.703314 5.2:+33114767 MS.gene55349:CDS
GAACAAGTACTGCTTACGAG+GGG 0.743478 5.2:+33114719 MS.gene55349:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGTCCAATTAAAAAAAT+TGG + chr5.2:33114270-33114289 MS.gene55349:intron 15.0%
!! TTTGAATGGTTAATATATTT+CGG + chr5.2:33114529-33114548 MS.gene55349:intron 15.0%
!! AGTTTCATTTCATGAAATTT+AGG - chr5.2:33113936-33113955 None:intergenic 20.0%
!! ATTGACAAAAATGTAAGAAT+TGG + chr5.2:33114443-33114462 MS.gene55349:intron 20.0%
!! CTTTAATTCTTAAGAATCAT+AGG - chr5.2:33113680-33113699 None:intergenic 20.0%
!! TAGCCTAAAAAAATTATAAG+AGG - chr5.2:33114360-33114379 None:intergenic 20.0%
!! TTATGATTATTATTTCACAG+AGG + chr5.2:33113969-33113988 MS.gene55349:intron 20.0%
! AAATCATGTATGAGAAATGT+CGG - chr5.2:33114229-33114248 None:intergenic 25.0%
! AATACATCATATCAGTAACT+AGG + chr5.2:33114390-33114409 MS.gene55349:intron 25.0%
! ACCATTCAAAAATAAAGTCA+TGG - chr5.2:33114519-33114538 None:intergenic 25.0%
! AGTTAATACTGACGAATAAT+AGG - chr5.2:33114609-33114628 None:intergenic 25.0%
! CTCACAAAAAAGAATATTAC+AGG - chr5.2:33114482-33114501 None:intergenic 25.0%
! TCCAATTAAAAAAATTGGCT+CGG + chr5.2:33114275-33114294 MS.gene55349:intron 25.0%
!! TAAGATAGTTTGAGTTAGTT+AGG + chr5.2:33113555-33113574 MS.gene55349:intron 25.0%
!!! ACCATGACTTTATTTTTGAA+TGG + chr5.2:33114515-33114534 MS.gene55349:intron 25.0%
!!! ATTTCTGTGTTTTATTCTTC+AGG + chr5.2:33114679-33114698 MS.gene55349:intron 25.0%
!!! GAGCCTCTTATAATTTTTTT+AGG + chr5.2:33114354-33114373 MS.gene55349:intron 25.0%
ATATGAGATAGAGATAGCAT+AGG - chr5.2:33113633-33113652 None:intergenic 30.0%
CAAATTCATAGCAATGTTGA+AGG + chr5.2:33114797-33114816 MS.gene55349:CDS 30.0%
GACATTTCTCATACATGATT+TGG + chr5.2:33114228-33114247 MS.gene55349:intron 30.0%
GTAAGAATGATGAGAAGATA+AGG - chr5.2:33113293-33113312 None:intergenic 30.0%
TCTTCAAAAATCAGTTCCTT+TGG + chr5.2:33113318-33113337 MS.gene55349:CDS 30.0%
TGAAACCATTAAAACGTTTG+AGG - chr5.2:33113876-33113895 None:intergenic 30.0%
! AATGAAAGATGGTAATGAAG+AGG - chr5.2:33113182-33113201 None:intergenic 30.0%
! CATTTTCAGCATATTTCTTC+TGG - chr5.2:33113999-33114018 None:intergenic 30.0%
!! TTTTATTTAGACAAAGTCGC+AGG + chr5.2:33114121-33114140 MS.gene55349:intron 30.0%
!!! TCCGAGCCAATTTTTTTAAT+TGG - chr5.2:33114279-33114298 None:intergenic 30.0%
AGATAAGGAGCAAGAAAACT+TGG - chr5.2:33113278-33113297 None:intergenic 35.0%
ATAGGATCTAATGCATACAC+CGG - chr5.2:33114591-33114610 None:intergenic 35.0%
CTGCATTAAAGCATGGTAAA+AGG - chr5.2:33113389-33113408 None:intergenic 35.0%
CTTCTATTGCAATTCTTCTC+TGG + chr5.2:33113344-33113363 MS.gene55349:CDS 35.0%
GTGGAAATCTGAATGAAAGA+TGG - chr5.2:33113193-33113212 None:intergenic 35.0%
TGACGATTATGATTGATCGA+CGG + chr5.2:33113719-33113738 MS.gene55349:intron 35.0%
TTATAAGAGGCTCACTCAAT+AGG - chr5.2:33114347-33114366 None:intergenic 35.0%
! AAAGTGCTATGTCGATATGT+CGG + chr5.2:33114189-33114208 MS.gene55349:intron 35.0%
! ATTGCTGAGTTTTTCACAGA+AGG - chr5.2:33113215-33113234 None:intergenic 35.0%
! GACTTCCTCAAACGTTTTAA+TGG + chr5.2:33113868-33113887 MS.gene55349:CDS 35.0%
! TCATACATGATTTGGACATG+AGG + chr5.2:33114236-33114255 MS.gene55349:intron 35.0%
! TGGTAAAAGGTTACTTTACC+TGG - chr5.2:33113376-33113395 None:intergenic 35.0%
AAGATTTAAAGAGACACCCC+TGG + chr5.2:33114023-33114042 MS.gene55349:CDS 40.0%
ACTCAGCAATCCTCAAAAGA+AGG + chr5.2:33113225-33113244 MS.gene55349:CDS 40.0%
AGTCACTAAGACACATGCTA+AGG + chr5.2:33113420-33113439 MS.gene55349:intron 40.0%
ATTGCAATAGAAGCAGCCAA+AGG - chr5.2:33113337-33113356 None:intergenic 40.0%
CATAGCAATGTTGAAGGAGA+AGG + chr5.2:33114803-33114822 MS.gene55349:CDS 40.0%
CCTCAAAAGAAGGTTCAGTT+TGG + chr5.2:33113235-33113254 MS.gene55349:CDS 40.0%
TTGCATGCTGCATTAAAGCA+TGG - chr5.2:33113396-33113415 None:intergenic 40.0%
! AGAACAAGTACTGCTTACGA+GGG + chr5.2:33114718-33114737 MS.gene55349:CDS 40.0%
! CCAAACTGAACCTTCTTTTG+AGG - chr5.2:33113238-33113257 None:intergenic 40.0%
! TCGATATGTCGGATACGTTT+TGG + chr5.2:33114200-33114219 MS.gene55349:intron 40.0%
!!! TCTTATTTTGCAGCACATGC+TGG + chr5.2:33113787-33113806 MS.gene55349:intron 40.0%
ATCTAATGCATACACCGGAC+AGG - chr5.2:33114586-33114605 None:intergenic 45.0%
ATGAATTTGTCCCTTGCCTC+TGG - chr5.2:33114786-33114805 None:intergenic 45.0%
ATTCTTCTCTGGTCAACTCC+AGG + chr5.2:33113355-33113374 MS.gene55349:CDS 45.0%
CGTTTGAGGAAGTCAATCTG+AGG - chr5.2:33113862-33113881 None:intergenic 45.0%
CTCAAGCAGTTTCTTCACCA+GGG - chr5.2:33114043-33114062 None:intergenic 45.0%
GCAATGTTGAAGGAGAAGGT+TGG + chr5.2:33114807-33114826 MS.gene55349:CDS 45.0%
GGATTCATGTCAGGAATCAC+AGG + chr5.2:33113808-33113827 MS.gene55349:CDS 45.0%
TCAAGCAGTTTCTTCACCAG+GGG - chr5.2:33114042-33114061 None:intergenic 45.0%
! GAACAAGTACTGCTTACGAG+GGG + chr5.2:33114719-33114738 MS.gene55349:CDS 45.0%
! GAGAACAAGTACTGCTTACG+AGG + chr5.2:33114717-33114736 MS.gene55349:CDS 45.0%
! GCTGAGTTTTTCACAGAAGG+TGG - chr5.2:33113212-33113231 None:intergenic 45.0%
!! AGTTGGTGATTGTTCAGCAG+AGG + chr5.2:33114752-33114771 MS.gene55349:CDS 45.0%
!! GTTGGTGATTGTTCAGCAGA+GGG + chr5.2:33114753-33114772 MS.gene55349:CDS 45.0%
!! TCACTCAATAGGTGTTGGCT+CGG - chr5.2:33114336-33114355 None:intergenic 45.0%
ACAGGACTAGAGTCTGTTCC+TGG + chr5.2:33113826-33113845 MS.gene55349:CDS 50.0%
CAAAGAGAGACACCGCAACA+CGG - chr5.2:33114562-33114581 None:intergenic 50.0%
GAGGCTCACTCAATAGGTGT+TGG - chr5.2:33114341-33114360 None:intergenic 50.0%
GCACATGCTGGATTCATGTC+AGG + chr5.2:33113799-33113818 MS.gene55349:intron 50.0%
GCTCAAGCAGTTTCTTCACC+AGG - chr5.2:33114044-33114063 None:intergenic 50.0%
GTGTCCAAGACGTGTCTAAC+AGG - chr5.2:33114302-33114321 None:intergenic 50.0%
GTGTCTCTCTTTGTCCTGTC+CGG + chr5.2:33114569-33114588 MS.gene55349:intron 50.0%
! AAGTCAATCTGAGGCAGCTC+AGG - chr5.2:33113853-33113872 None:intergenic 50.0%
AGCAGAGGGAATGACTCCAG+AGG + chr5.2:33114767-33114786 MS.gene55349:CDS 55.0%
AGGGAATGACTCCAGAGGCA+AGG + chr5.2:33114772-33114791 MS.gene55349:CDS 55.0%
CGGACCTGTTAGACACGTCT+TGG + chr5.2:33114295-33114314 MS.gene55349:intron 55.0%
GGGAATGACTCCAGAGGCAA+GGG + chr5.2:33114773-33114792 MS.gene55349:CDS 55.0%
TTCGGACACAAGCCGTGTTG+CGG + chr5.2:33114547-33114566 MS.gene55349:intron 55.0%
! CAAGTACTGCTTACGAGGGG+CGG + chr5.2:33114722-33114741 MS.gene55349:CDS 55.0%
ACTGCTTACGAGGGGCGGAA+TGG + chr5.2:33114727-33114746 MS.gene55349:CDS 60.0%
CTGCTTACGAGGGGCGGAAT+GGG + chr5.2:33114728-33114747 MS.gene55349:CDS 60.0%
TCGCAGGCAGCTGCAAGTGA+TGG + chr5.2:33114137-33114156 MS.gene55349:intron 60.0%
TGCTTACGAGGGGCGGAATG+GGG + chr5.2:33114729-33114748 MS.gene55349:CDS 60.0%
! ATCTGAGGCAGCTCAGGACC+AGG - chr5.2:33113847-33113866 None:intergenic 60.0%
CGAGGGGCGGAATGGGGAGT+TGG + chr5.2:33114735-33114754 MS.gene55349:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 33113172 33114839 33113172 ID=MS.gene55349
chr5.2 mRNA 33113172 33114839 33113172 ID=MS.gene55349.t1;Parent=MS.gene55349
chr5.2 exon 33113172 33113376 33113172 ID=MS.gene55349.t1.exon1;Parent=MS.gene55349.t1
chr5.2 CDS 33113172 33113376 33113172 ID=cds.MS.gene55349.t1;Parent=MS.gene55349.t1
chr5.2 exon 33113800 33113889 33113800 ID=MS.gene55349.t1.exon2;Parent=MS.gene55349.t1
chr5.2 CDS 33113800 33113889 33113800 ID=cds.MS.gene55349.t1;Parent=MS.gene55349.t1
chr5.2 exon 33113989 33114085 33113989 ID=MS.gene55349.t1.exon3;Parent=MS.gene55349.t1
chr5.2 CDS 33113989 33114085 33113989 ID=cds.MS.gene55349.t1;Parent=MS.gene55349.t1
chr5.2 exon 33114701 33114839 33114701 ID=MS.gene55349.t1.exon4;Parent=MS.gene55349.t1
chr5.2 CDS 33114701 33114839 33114701 ID=cds.MS.gene55349.t1;Parent=MS.gene55349.t1
Gene Sequence

>MS.gene55349

ATGGGTTCCTCTTCATTACCATCTTTCATTCAGATTTCCACCTTCTGTGAAAAACTCAGCAATCCTCAAAAGAAGGTTCAGTTTGGAATGACAAAAAATGACACCAAGTTTTCTTGCTCCTTATCTTCTCATCATTCTTACAATATTCTTCAAAAATCAGTTCCTTTGGCTGCTTCTATTGCAATTCTTCTCTGGTCAACTCCAGGTAAAGTAACCTTTTACCATGCTTTAATGCAGCATGCAATTCAAGTCACTAAGACACATGCTAAGGCATTTTTGTTATTGAGTTTAATATTATATACTGACAAAGTAAAATAGTTTTACAGTGTCATCAAATCATAATCAATCATATATATGATTTTACAAGTAGTTATGATTGATGTTAAGATAGTTTGAGTTAGTTAGGATTAGTTAGAGTTAGTTACTCGTTAGCGTAGTTGAGTTCTATAAATAGTATTCCTATGCTATCTCTATCTCATATGAGTATGATCTGCAGCACTATGATCCTATGATTCTTAAGAATTAAAGTGAAACATTTTTAATAATCTGACGATTATGATTGATCGACGGTGTAAAAAATATTTACTGTGACAATGTATATGAATTGAATTTTTTTCTTATTTTGCAGCACATGCTGGATTCATGTCAGGAATCACAGGACTAGAGTCTGTTCCTGGTCCTGAGCTGCCTCAGATTGACTTCCTCAAACGTTTTAATGGTTTCAAACTTCAAACTCTTATAGCATGTTGTGATTTTGTAAACCTAAATTTCATGAAATGAAACTAACATATATTGTTTTATGATTATTATTTCACAGAGGAAAACCAGAAGAAATATGCTGAAAATGATGCAAGATTTAAAGAGACACCCCTGGTGAAGAAACTGCTTGAGCAATCTAAGTTGAATAAAGAGAAGTAATTTTTCTGTTATCATAATTGAACTGTGTATTTTTTATTTAGACAAAGTCGCAGGCAGCTGCAAGTGATGGCAGATAGCTAAGAATACTAATATCTTTCTAAAGTGCTATGTCGATATGTCGGATACGTTTTGGACACCGACATTTCTCATACATGATTTGGACATGAGGTAGACACTTCTTAATGTCCAATTAAAAAAATTGGCTCGGACCTGTTAGACACGTCTTGGACACACGTCAAGAGACCGAGCCAACACCTATTGAGTGAGCCTCTTATAATTTTTTTAGGCTATTAAGTTAACAATACATCATATCAGTAACTAGGTTTTATAAAACACACTATCTGTCTTCTTATATTGACAAAAATGTAAGAATTGGATAGTAAATGATTCCTGTAATATTCTTTTTTGTGAGAATCTTTGTCATTACCATGACTTTATTTTTGAATGGTTAATATATTTCGGACACAAGCCGTGTTGCGGTGTCTCTCTTTGTCCTGTCCGGTGTATGCATTAGATCCTATTATTCGTCAGTATTAACTTTGTTTAAAACAATGTTTCTATGCATCTGTTATCAGAAAGTTACAACAACATTTCTGTGTTTTATTCTTCAGGAATAGCAAAGAAATTGAGAACAAGTACTGCTTACGAGGGGCGGAATGGGGAGTTGGTGATTGTTCAGCAGAGGGAATGACTCCAGAGGCAAGGGACAAATTCATAGCAATGTTGAAGGAGAAGGTTGGAGAAAAATAA

Protein sequence

>MS.gene55349.t1

MGSSSLPSFIQISTFCEKLSNPQKKVQFGMTKNDTKFSCSLSSHHSYNILQKSVPLAASIAILLWSTPAHAGFMSGITGLESVPGPELPQIDFLKRFNEENQKKYAENDARFKETPLVKKLLEQSKLNKEKNSKEIENKYCLRGAEWGVGDCSAEGMTPEARDKFIAMLKEKVGEK