AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20025


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20025.t1 MTR_7g117900 88.119 202 19 1 1 202 1 197 4.49e-126 355
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20025.t1 AT4G00895 40.945 127 61 3 65 187 75 191 2.37e-19 82.4

Find 54 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAAGAGAAATGAGATTT+TGG 0.211304 7.4:-1065760 None:intergenic
GGTTTCATCAAAAGAGAAAT+TGG 0.262334 7.4:+1066197 MS.gene20025:CDS
ACTGAGTTTCTGCACACTTT+TGG 0.309140 7.4:-1066243 None:intergenic
ATGTGGAACTCAAGTTGTTT+TGG 0.349249 7.4:+1066176 MS.gene20025:CDS
ATGGTTTGTGTTTGAGAGGT+TGG 0.358792 7.4:-1065849 None:intergenic
ATTGGTGAAGATGAAATGTT+TGG 0.381129 7.4:+1066215 MS.gene20025:CDS
TGGTTTGTGTTTGAGAGGTT+GGG 0.403119 7.4:-1065848 None:intergenic
TTTGAGAGGTTGGGTCTTTG+AGG 0.403625 7.4:-1065839 None:intergenic
GCAGCAGCATAGCCAGTAGC+AGG 0.415721 7.4:-1065898 None:intergenic
TGAAGGGCATGTGGTTGGTT+TGG 0.422427 7.4:+1066074 MS.gene20025:CDS
TGTTGGTGTTGACCCTTCAT+TGG 0.430284 7.4:+1066023 MS.gene20025:CDS
TTGAGAGGTTGGGTCTTTGA+GGG 0.430753 7.4:-1065838 None:intergenic
ACCACCTTCCTCACCAATGA+AGG 0.445507 7.4:-1066036 None:intergenic
TTGTGAAGTGATAGAATTCA+CGG 0.447151 7.4:-1065696 None:intergenic
CACCCTTCCCTCGGTTCAAA+GGG 0.453751 7.4:+1065948 MS.gene20025:CDS
TTCCATCACAAGCCTGCTAC+TGG 0.468777 7.4:+1065886 MS.gene20025:CDS
TTGGTTGTTGAGGATGAGAA+GGG 0.471694 7.4:-1065741 None:intergenic
TTTGGTTGTTGAGGATGAGA+AGG 0.473648 7.4:-1065742 None:intergenic
ACACCCTTCCCTCGGTTCAA+AGG 0.483662 7.4:+1065947 MS.gene20025:CDS
TAGAATTCACGGTTGGTTGT+TGG 0.484183 7.4:-1065685 None:intergenic
TTGGTGAAGATGAAATGTTT+GGG 0.494512 7.4:+1066216 MS.gene20025:CDS
TTGAACCGAGGGAAGGGTGT+TGG 0.514330 7.4:-1065944 None:intergenic
TGGGTCTTTGAGGGTAGTGA+AGG 0.516568 7.4:-1065829 None:intergenic
AATTCACGGTTGGTTGTTGG+TGG 0.518266 7.4:-1065682 None:intergenic
AGCCAGTAGCAGGCTTGTGA+TGG 0.522904 7.4:-1065888 None:intergenic
AGGATTTATGATGAATTATG+TGG 0.523605 7.4:+1066159 MS.gene20025:CDS
GAAGATGGTTTGTGTTTGAG+AGG 0.530277 7.4:-1065853 None:intergenic
TGGAATACTTGAGAAGAAGA+TGG 0.533703 7.4:-1065868 None:intergenic
AAATTTGAAGGGCATGTGGT+TGG 0.536206 7.4:+1066069 MS.gene20025:CDS
AAGTGTTGGAAGAGTTCATG+AGG 0.536714 7.4:+1066139 MS.gene20025:CDS
TGACCCTTCATTGGTGAGGA+AGG 0.538931 7.4:+1066032 MS.gene20025:CDS
GTGGAGCAGAGAAAATTTGA+AGG 0.540648 7.4:+1066057 MS.gene20025:CDS
GGGAATTGTTGAGGAAGTGT+TGG 0.544791 7.4:+1066125 MS.gene20025:CDS
ATCCCTTTGAACCGAGGGAA+GGG 0.545984 7.4:-1065950 None:intergenic
GCTGATGAAGAAGAACAAGC+TGG 0.546081 7.4:+1066104 MS.gene20025:CDS
AAATTTCAATCAGTTGATGT+TGG 0.559351 7.4:+1066006 MS.gene20025:CDS
GAAACTCAGTGGTGCAGTGA+GGG 0.562635 7.4:+1066256 MS.gene20025:CDS
AGAGCTTGAGAAAGCATCCA+TGG 0.563193 7.4:-1065641 None:intergenic
ATTCACGGTTGGTTGTTGGT+GGG 0.565174 7.4:-1065681 None:intergenic
TTCATTGGTGAGGAAGGTGG+TGG 0.567833 7.4:+1066038 MS.gene20025:CDS
CATCCCTTTGAACCGAGGGA+AGG 0.585509 7.4:-1065951 None:intergenic
CCCTTCATTGGTGAGGAAGG+TGG 0.586166 7.4:+1066035 MS.gene20025:CDS
CTGATGAAGAAGAACAAGCT+GGG 0.589597 7.4:+1066105 MS.gene20025:CDS
GAGAAAATTTGAAGGGCATG+TGG 0.597790 7.4:+1066065 MS.gene20025:CDS
TGAACATCCCTTTGAACCGA+GGG 0.602693 7.4:-1065955 None:intergenic
GTGTTGACCCTTCATTGGTG+AGG 0.604863 7.4:+1066028 MS.gene20025:CDS
TTGAACATCCCTTTGAACCG+AGG 0.616637 7.4:-1065956 None:intergenic
GAAGGGTTTGCAGTTGCATG+AGG 0.616977 7.4:-1065724 None:intergenic
GAACAAGCTGGGAATTGTTG+AGG 0.625515 7.4:+1066116 MS.gene20025:CDS
CCACCTTCCTCACCAATGAA+GGG 0.627070 7.4:-1066035 None:intergenic
GAAGTGATAGAATTCACGGT+TGG 0.666630 7.4:-1065692 None:intergenic
TGGAGCAGAGAAAATTTGAA+GGG 0.672649 7.4:+1066058 MS.gene20025:CDS
AGAAACTCAGTGGTGCAGTG+AGG 0.695602 7.4:+1066255 MS.gene20025:CDS
AAAAGTGTGCAGAAACTCAG+TGG 0.740335 7.4:+1066245 MS.gene20025:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAGAAAGTTTTTGTTTTTT+GGG - chr7.4:1065794-1065813 None:intergenic 20.0%
! AGGATTTATGATGAATTATG+TGG + chr7.4:1066159-1066178 MS.gene20025:CDS 25.0%
!! AAATTTCAATCAGTTGATGT+TGG + chr7.4:1066006-1066025 MS.gene20025:CDS 25.0%
!! CTGATTGAAATTTCAGTTTT+TGG - chr7.4:1065999-1066018 None:intergenic 25.0%
!!! ATTGAAATTTCAGTTTTTGG+AGG - chr7.4:1065996-1066015 None:intergenic 25.0%
!!! GTGAGAAAGTTTTTGTTTTT+TGG - chr7.4:1065795-1065814 None:intergenic 25.0%
ATTGGTGAAGATGAAATGTT+TGG + chr7.4:1066215-1066234 MS.gene20025:CDS 30.0%
GGTTTCATCAAAAGAGAAAT+TGG + chr7.4:1066197-1066216 MS.gene20025:CDS 30.0%
TTGGTGAAGATGAAATGTTT+GGG + chr7.4:1066216-1066235 MS.gene20025:CDS 30.0%
TTGTGAAGTGATAGAATTCA+CGG - chr7.4:1065699-1065718 None:intergenic 30.0%
! TTGGAAGAGAAATGAGATTT+TGG - chr7.4:1065763-1065782 None:intergenic 30.0%
!!! AAATGAGATTTTGGTTGTTG+AGG - chr7.4:1065754-1065773 None:intergenic 30.0%
TGGAATACTTGAGAAGAAGA+TGG - chr7.4:1065871-1065890 None:intergenic 35.0%
TGGAGCAGAGAAAATTTGAA+GGG + chr7.4:1066058-1066077 MS.gene20025:CDS 35.0%
!! ATGTGGAACTCAAGTTGTTT+TGG + chr7.4:1066176-1066195 MS.gene20025:CDS 35.0%
!!! TGTTTTTTGGGTGACTAAGT+TGG - chr7.4:1065782-1065801 None:intergenic 35.0%
CTGATGAAGAAGAACAAGCT+GGG + chr7.4:1066105-1066124 MS.gene20025:CDS 40.0%
GAAGTGATAGAATTCACGGT+TGG - chr7.4:1065695-1065714 None:intergenic 40.0%
GTGGAGCAGAGAAAATTTGA+AGG + chr7.4:1066057-1066076 MS.gene20025:CDS 40.0%
TTGGTTGTTGAGGATGAGAA+GGG - chr7.4:1065744-1065763 None:intergenic 40.0%
TTTGGTTGTTGAGGATGAGA+AGG - chr7.4:1065745-1065764 None:intergenic 40.0%
! AAAAGTGTGCAGAAACTCAG+TGG + chr7.4:1066245-1066264 MS.gene20025:CDS 40.0%
! AAATTTGAAGGGCATGTGGT+TGG + chr7.4:1066069-1066088 MS.gene20025:CDS 40.0%
! ACTGAGTTTCTGCACACTTT+TGG - chr7.4:1066246-1066265 None:intergenic 40.0%
! ATGGTTTGTGTTTGAGAGGT+TGG - chr7.4:1065852-1065871 None:intergenic 40.0%
! GAAGATGGTTTGTGTTTGAG+AGG - chr7.4:1065856-1065875 None:intergenic 40.0%
! GAGAAAATTTGAAGGGCATG+TGG + chr7.4:1066065-1066084 MS.gene20025:CDS 40.0%
! TAGAATTCACGGTTGGTTGT+TGG - chr7.4:1065688-1065707 None:intergenic 40.0%
! TGGTTTGTGTTTGAGAGGTT+GGG - chr7.4:1065851-1065870 None:intergenic 40.0%
!! AAGTGTTGGAAGAGTTCATG+AGG + chr7.4:1066139-1066158 MS.gene20025:CDS 40.0%
AAAAACCAACACCCTTCCCT+CGG + chr7.4:1065939-1065958 MS.gene20025:CDS 45.0%
GAACAAGCTGGGAATTGTTG+AGG + chr7.4:1066116-1066135 MS.gene20025:CDS 45.0%
GCTGATGAAGAAGAACAAGC+TGG + chr7.4:1066104-1066123 MS.gene20025:CDS 45.0%
TGAACATCCCTTTGAACCGA+GGG - chr7.4:1065958-1065977 None:intergenic 45.0%
TTGAACATCCCTTTGAACCG+AGG - chr7.4:1065959-1065978 None:intergenic 45.0%
TTGAGAGGTTGGGTCTTTGA+GGG - chr7.4:1065841-1065860 None:intergenic 45.0%
TTTGAGAGGTTGGGTCTTTG+AGG - chr7.4:1065842-1065861 None:intergenic 45.0%
! AATTCACGGTTGGTTGTTGG+TGG - chr7.4:1065685-1065704 None:intergenic 45.0%
! ATTCACGGTTGGTTGTTGGT+GGG - chr7.4:1065684-1065703 None:intergenic 45.0%
! GGGAATTGTTGAGGAAGTGT+TGG + chr7.4:1066125-1066144 MS.gene20025:CDS 45.0%
!! GGTTGTTGGTGGGATTTTGA+GGG - chr7.4:1065674-1065693 None:intergenic 45.0%
!! TGGTTGTTGGTGGGATTTTG+AGG - chr7.4:1065675-1065694 None:intergenic 45.0%
!! TGTTGGTGTTGACCCTTCAT+TGG + chr7.4:1066023-1066042 MS.gene20025:CDS 45.0%
ACCACCTTCCTCACCAATGA+AGG - chr7.4:1066039-1066058 None:intergenic 50.0%
ATCCCTTTGAACCGAGGGAA+GGG - chr7.4:1065953-1065972 None:intergenic 50.0%
CCACCTTCCTCACCAATGAA+GGG - chr7.4:1066038-1066057 None:intergenic 50.0%
GAAGGGTTTGCAGTTGCATG+AGG - chr7.4:1065727-1065746 None:intergenic 50.0%
TGACCCTTCATTGGTGAGGA+AGG + chr7.4:1066032-1066051 MS.gene20025:CDS 50.0%
TTCATTGGTGAGGAAGGTGG+TGG + chr7.4:1066038-1066057 MS.gene20025:CDS 50.0%
TTCCATCACAAGCCTGCTAC+TGG + chr7.4:1065886-1065905 MS.gene20025:CDS 50.0%
! GTGTTGACCCTTCATTGGTG+AGG + chr7.4:1066028-1066047 MS.gene20025:CDS 50.0%
! TGAAGGGCATGTGGTTGGTT+TGG + chr7.4:1066074-1066093 MS.gene20025:CDS 50.0%
! TGGGTCTTTGAGGGTAGTGA+AGG - chr7.4:1065832-1065851 None:intergenic 50.0%
ACACCCTTCCCTCGGTTCAA+AGG + chr7.4:1065947-1065966 MS.gene20025:CDS 55.0%
AGCCAGTAGCAGGCTTGTGA+TGG - chr7.4:1065891-1065910 None:intergenic 55.0%
CACCCTTCCCTCGGTTCAAA+GGG + chr7.4:1065948-1065967 MS.gene20025:CDS 55.0%
CATCCCTTTGAACCGAGGGA+AGG - chr7.4:1065954-1065973 None:intergenic 55.0%
CCCTTCATTGGTGAGGAAGG+TGG + chr7.4:1066035-1066054 MS.gene20025:CDS 55.0%
! TTGAACCGAGGGAAGGGTGT+TGG - chr7.4:1065947-1065966 None:intergenic 55.0%
GCAGCAGCATAGCCAGTAGC+AGG - chr7.4:1065901-1065920 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 1065643 1066275 1065643 ID=MS.gene20025
chr7.4 mRNA 1065643 1066275 1065643 ID=MS.gene20025.t1;Parent=MS.gene20025
chr7.4 exon 1065643 1066275 1065643 ID=MS.gene20025.t1.exon1;Parent=MS.gene20025.t1
chr7.4 CDS 1065643 1066275 1065643 ID=cds.MS.gene20025.t1;Parent=MS.gene20025.t1
Gene Sequence

>MS.gene20025

ATGGATGCTTTCTCAAGCTCTGTTTCAACCCTCAAAATCCCACCAACAACCAACCGTGAATTCTATCACTTCACAAGAACTCCTCATGCAACTGCAAACCCTTCTCATCCTCAACAACCAAAATCTCATTTCTCTTCCAACTTAGTCACCCAAAAAACAAAAACTTTCTCACACAACAATCATTCTCCTTCACTACCCTCAAAGACCCAACCTCTCAAACACAAACCATCTTCTTCTCAAGTATTCCATCACAAGCCTGCTACTGGCTATGCTGCTGCAATCATAGATGTAGCTCAAAAAACCAACACCCTTCCCTCGGTTCAAAGGGATGTTCAAAGACTCTTGAAGTTCCTCCAAAAACTGAAATTTCAATCAGTTGATGTTGGTGTTGACCCTTCATTGGTGAGGAAGGTGGTGGAGCAGAGAAAATTTGAAGGGCATGTGGTTGGTTTGGTGAAGATGCTGATGAAGAAGAACAAGCTGGGAATTGTTGAGGAAGTGTTGGAAGAGTTCATGAGGATTTATGATGAATTATGTGGAACTCAAGTTGTTTTGGTTTCATCAAAAGAGAAATTGGTGAAGATGAAATGTTTGGGATTGCCAAAAGTGTGCAGAAACTCAGTGGTGCAGTGA

Protein sequence

>MS.gene20025.t1

MDAFSSSVSTLKIPPTTNREFYHFTRTPHATANPSHPQQPKSHFSSNLVTQKTKTFSHNNHSPSLPSKTQPLKHKPSSSQVFHHKPATGYAAAIIDVAQKTNTLPSVQRDVQRLLKFLQKLKFQSVDVGVDPSLVRKVVEQRKFEGHVVGLVKMLMKKNKLGIVEEVLEEFMRIYDELCGTQVVLVSSKEKLVKMKCLGLPKVCRNSVVQ