Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95885.t1 | XP_003631061.1 | 98.3 | 176 | 3 | 0 | 1 | 176 | 57 | 232 | 3.70E-95 | 357.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95885.t1 | G7LD88 | 98.3 | 176 | 3 | 0 | 1 | 176 | 57 | 232 | 2.6e-95 | 357.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049134 | MS.gene95885 | -0.820195 | 7.80E-53 | -1.69E-46 |
| MS.gene049137 | MS.gene95885 | 0.804815 | 1.80E-49 | -1.69E-46 |
| MS.gene049150 | MS.gene95885 | 0.807903 | 4.03E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene95885 | 0.811252 | 7.68E-51 | -1.69E-46 |
| MS.gene04933 | MS.gene95885 | 0.802062 | 6.70E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene95885 | 0.809325 | 2.00E-50 | -1.69E-46 |
| MS.gene049351 | MS.gene95885 | 0.852307 | 5.03E-61 | -1.69E-46 |
| MS.gene049356 | MS.gene95885 | 0.834858 | 2.35E-56 | -1.69E-46 |
| MS.gene049369 | MS.gene95885 | 0.803873 | 2.83E-49 | -1.69E-46 |
| MS.gene049661 | MS.gene95885 | 0.835211 | 1.91E-56 | -1.69E-46 |
| MS.gene049662 | MS.gene95885 | 0.832133 | 1.12E-55 | -1.69E-46 |
| MS.gene049758 | MS.gene95885 | 0.848357 | 6.46E-60 | -1.69E-46 |
| MS.gene049805 | MS.gene95885 | 0.836927 | 7.02E-57 | -1.69E-46 |
| MS.gene049910 | MS.gene95885 | 0.846451 | 2.15E-59 | -1.69E-46 |
| MS.gene049993 | MS.gene95885 | 0.814848 | 1.25E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene95885 | 0.836596 | 8.53E-57 | -1.69E-46 |
| MS.gene050231 | MS.gene95885 | 0.819856 | 9.32E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene95885 | 0.867066 | 1.80E-65 | -1.69E-46 |
| MS.gene050397 | MS.gene95885 | 0.838641 | 2.55E-57 | -1.69E-46 |
| MS.gene050426 | MS.gene95885 | -0.806491 | 8.02E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene95885 | 0.851145 | 1.07E-60 | -1.69E-46 |
| MS.gene050464 | MS.gene95885 | 0.840959 | 6.35E-58 | -1.69E-46 |
| MS.gene050529 | MS.gene95885 | 0.841575 | 4.37E-58 | -1.69E-46 |
| MS.gene050593 | MS.gene95885 | 0.815533 | 8.80E-52 | -1.69E-46 |
| MS.gene050670 | MS.gene95885 | 0.8844 | 1.98E-71 | -1.69E-46 |
| MS.gene05101 | MS.gene95885 | 0.827379 | 1.62E-54 | -1.69E-46 |
| MS.gene05105 | MS.gene95885 | 0.858524 | 7.76E-63 | -1.69E-46 |
| MS.gene051360 | MS.gene95885 | 0.80435 | 2.25E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene95885 | 0.844851 | 5.85E-59 | -1.69E-46 |
| MS.gene051471 | MS.gene95885 | 0.812147 | 4.91E-51 | -1.69E-46 |
| MS.gene051525 | MS.gene95885 | 0.875621 | 2.67E-68 | -1.69E-46 |
| MS.gene051582 | MS.gene95885 | 0.878141 | 3.57E-69 | -1.69E-46 |
| MS.gene051692 | MS.gene95885 | 0.80446 | 2.14E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene95885 | 0.839691 | 1.36E-57 | -1.69E-46 |
| MS.gene051877 | MS.gene95885 | 0.849465 | 3.18E-60 | -1.69E-46 |
| MS.gene052011 | MS.gene95885 | -0.86037 | 2.17E-63 | -1.69E-46 |
| MS.gene052077 | MS.gene95885 | 0.840366 | 9.08E-58 | -1.69E-46 |
| MS.gene052078 | MS.gene95885 | 0.834794 | 2.44E-56 | -1.69E-46 |
| MS.gene052134 | MS.gene95885 | 0.810543 | 1.09E-50 | -1.69E-46 |
| MS.gene052135 | MS.gene95885 | 0.811404 | 7.12E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene95885 | 0.856652 | 2.78E-62 | -1.69E-46 |
| MS.gene052253 | MS.gene95885 | 0.868034 | 8.81E-66 | -1.69E-46 |
| MS.gene052310 | MS.gene95885 | 0.865155 | 7.25E-65 | -1.69E-46 |
| MS.gene052322 | MS.gene95885 | 0.817884 | 2.62E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene95885 | 0.858983 | 5.66E-63 | -1.69E-46 |
| MS.gene052414 | MS.gene95885 | 0.806853 | 6.72E-50 | -1.69E-46 |
| MS.gene052421 | MS.gene95885 | 0.869526 | 2.90E-66 | -1.69E-46 |
| MS.gene052481 | MS.gene95885 | 0.840044 | 1.10E-57 | -1.69E-46 |
| MS.gene052484 | MS.gene95885 | 0.811305 | 7.48E-51 | -1.69E-46 |
| MS.gene052485 | MS.gene95885 | 0.866 | 3.93E-65 | -1.69E-46 |
| MS.gene052505 | MS.gene95885 | 0.814857 | 1.24E-51 | -1.69E-46 |
| MS.gene052767 | MS.gene95885 | 0.809766 | 1.61E-50 | -1.69E-46 |
| MS.gene052982 | MS.gene95885 | 0.848345 | 6.50E-60 | -1.69E-46 |
| MS.gene053103 | MS.gene95885 | 0.846177 | 2.56E-59 | -1.69E-46 |
| MS.gene053209 | MS.gene95885 | 0.853819 | 1.86E-61 | -1.69E-46 |
| MS.gene053322 | MS.gene95885 | 0.808356 | 3.22E-50 | -1.69E-46 |
| MS.gene053449 | MS.gene95885 | 0.809558 | 1.78E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene95885 | 0.807847 | 4.14E-50 | -1.69E-46 |
| MS.gene053813 | MS.gene95885 | 0.80361 | 3.21E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene95885 | 0.862658 | 4.34E-64 | -1.69E-46 |
| MS.gene053928 | MS.gene95885 | 0.839553 | 1.48E-57 | -1.69E-46 |
| MS.gene054010 | MS.gene95885 | 0.801708 | 7.93E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene95885 | 0.822691 | 2.07E-53 | -1.69E-46 |
| MS.gene054012 | MS.gene95885 | 0.807075 | 6.03E-50 | -1.69E-46 |
| MS.gene054013 | MS.gene95885 | 0.805156 | 1.53E-49 | -1.69E-46 |
| MS.gene054123 | MS.gene95885 | 0.819982 | 8.72E-53 | -1.69E-46 |
| MS.gene05412 | MS.gene95885 | 0.815786 | 7.73E-52 | -1.69E-46 |
| MS.gene054183 | MS.gene95885 | 0.849015 | 4.24E-60 | -1.69E-46 |
| MS.gene054349 | MS.gene95885 | 0.824331 | 8.55E-54 | -1.69E-46 |
| MS.gene054473 | MS.gene95885 | 0.837208 | 5.95E-57 | -1.69E-46 |
| MS.gene054556 | MS.gene95885 | 0.806083 | 9.77E-50 | -1.69E-46 |
| MS.gene054719 | MS.gene95885 | 0.841224 | 5.41E-58 | -1.69E-46 |
| MS.gene054918 | MS.gene95885 | 0.823945 | 1.05E-53 | -1.69E-46 |
| MS.gene054955 | MS.gene95885 | 0.806743 | 7.09E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene95885 | 0.843354 | 1.48E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene95885 | 0.86897 | 4.40E-66 | -1.69E-46 |
| MS.gene055277 | MS.gene95885 | 0.880517 | 5.14E-70 | -1.69E-46 |
| MS.gene055289 | MS.gene95885 | 0.805332 | 1.40E-49 | -1.69E-46 |
| MS.gene055369 | MS.gene95885 | 0.837677 | 4.51E-57 | -1.69E-46 |
| MS.gene055390 | MS.gene95885 | 0.835475 | 1.64E-56 | -1.69E-46 |
| MS.gene055412 | MS.gene95885 | 0.831002 | 2.14E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene95885 | 0.848814 | 4.82E-60 | -1.69E-46 |
| MS.gene055428 | MS.gene95885 | 0.852812 | 3.61E-61 | -1.69E-46 |
| MS.gene055486 | MS.gene95885 | 0.800883 | 1.17E-48 | -1.69E-46 |
| MS.gene05553 | MS.gene95885 | 0.863877 | 1.82E-64 | -1.69E-46 |
| MS.gene055604 | MS.gene95885 | 0.826411 | 2.75E-54 | -1.69E-46 |
| MS.gene055666 | MS.gene95885 | 0.807448 | 5.03E-50 | -1.69E-46 |
| MS.gene055911 | MS.gene95885 | 0.803654 | 3.14E-49 | -1.69E-46 |
| MS.gene05592 | MS.gene95885 | 0.807206 | 5.66E-50 | -1.69E-46 |
| MS.gene05594 | MS.gene95885 | 0.839379 | 1.64E-57 | -1.69E-46 |
| MS.gene056191 | MS.gene95885 | 0.840902 | 6.57E-58 | -1.69E-46 |
| MS.gene056407 | MS.gene95885 | 0.873083 | 1.94E-67 | -1.69E-46 |
| MS.gene056410 | MS.gene95885 | 0.808353 | 3.23E-50 | -1.69E-46 |
| MS.gene056497 | MS.gene95885 | 0.802683 | 4.99E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene95885 | 0.840938 | 6.43E-58 | -1.69E-46 |
| MS.gene056514 | MS.gene95885 | 0.811916 | 5.51E-51 | -1.69E-46 |
| MS.gene056612 | MS.gene95885 | 0.829919 | 3.93E-55 | -1.69E-46 |
| MS.gene056659 | MS.gene95885 | 0.824758 | 6.79E-54 | -1.69E-46 |
| MS.gene056723 | MS.gene95885 | 0.817487 | 3.22E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene95885 | -0.832168 | 1.10E-55 | -1.69E-46 |
| MS.gene056902 | MS.gene95885 | 0.808339 | 3.25E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene95885 | 0.830124 | 3.50E-55 | -1.69E-46 |
| MS.gene05702 | MS.gene95885 | 0.857268 | 1.83E-62 | -1.69E-46 |
| MS.gene057121 | MS.gene95885 | 0.814006 | 1.92E-51 | -1.69E-46 |
| MS.gene057123 | MS.gene95885 | 0.810496 | 1.12E-50 | -1.69E-46 |
| MS.gene057124 | MS.gene95885 | 0.864069 | 1.58E-64 | -1.69E-46 |
| MS.gene057146 | MS.gene95885 | 0.838852 | 2.25E-57 | -1.69E-46 |
| MS.gene057245 | MS.gene95885 | 0.803738 | 3.02E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene95885 | 0.821573 | 3.76E-53 | -1.69E-46 |
| MS.gene057396 | MS.gene95885 | 0.805909 | 1.06E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene95885 | 0.800424 | 1.45E-48 | -1.69E-46 |
| MS.gene057448 | MS.gene95885 | 0.820007 | 8.61E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene95885 | 0.802832 | 4.65E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene95885 | 0.82393 | 1.06E-53 | -1.69E-46 |
| MS.gene057469 | MS.gene95885 | 0.822299 | 2.55E-53 | -1.69E-46 |
| MS.gene057476 | MS.gene95885 | 0.811847 | 5.70E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene95885 | 0.86135 | 1.09E-63 | -1.69E-46 |
| MS.gene057823 | MS.gene95885 | 0.871779 | 5.27E-67 | -1.69E-46 |
| MS.gene057825 | MS.gene95885 | 0.814066 | 1.86E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene95885 | 0.855646 | 5.49E-62 | -1.69E-46 |
| MS.gene058006 | MS.gene95885 | 0.827895 | 1.22E-54 | -1.69E-46 |
| MS.gene058007 | MS.gene95885 | 0.810468 | 1.14E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene95885 | 0.816619 | 5.04E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene95885 | 0.851707 | 7.45E-61 | -1.69E-46 |
| MS.gene058142 | MS.gene95885 | 0.816815 | 4.55E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene95885 | 0.848593 | 5.55E-60 | -1.69E-46 |
| MS.gene058276 | MS.gene95885 | 0.806422 | 8.29E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene95885 | 0.860447 | 2.05E-63 | -1.69E-46 |
| MS.gene058365 | MS.gene95885 | 0.81473 | 1.33E-51 | -1.69E-46 |
| MS.gene058376 | MS.gene95885 | 0.808256 | 3.39E-50 | -1.69E-46 |
| MS.gene05842 | MS.gene95885 | 0.816168 | 6.35E-52 | -1.69E-46 |
| MS.gene058444 | MS.gene95885 | 0.816678 | 4.89E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene95885 | 0.852949 | 3.30E-61 | -1.69E-46 |
| MS.gene058700 | MS.gene95885 | 0.84286 | 2.00E-58 | -1.69E-46 |
| MS.gene058710 | MS.gene95885 | 0.815648 | 8.30E-52 | -1.69E-46 |
| MS.gene058787 | MS.gene95885 | 0.804825 | 1.79E-49 | -1.69E-46 |
| MS.gene05886 | MS.gene95885 | 0.856878 | 2.39E-62 | -1.69E-46 |
| MS.gene058928 | MS.gene95885 | 0.843692 | 1.20E-58 | -1.69E-46 |
| MS.gene059011 | MS.gene95885 | 0.809023 | 2.32E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene95885 | 0.871155 | 8.47E-67 | -1.69E-46 |
| MS.gene059082 | MS.gene95885 | 0.816217 | 6.20E-52 | -1.69E-46 |
| MS.gene059141 | MS.gene95885 | 0.83411 | 3.62E-56 | -1.69E-46 |
| MS.gene059285 | MS.gene95885 | 0.815653 | 8.28E-52 | -1.69E-46 |
| MS.gene059294 | MS.gene95885 | 0.803314 | 3.70E-49 | -1.69E-46 |
| MS.gene059366 | MS.gene95885 | 0.833289 | 5.81E-56 | -1.69E-46 |
| MS.gene059420 | MS.gene95885 | 0.900712 | 5.57E-78 | -1.69E-46 |
| MS.gene059462 | MS.gene95885 | 0.813528 | 2.45E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene95885 | 0.815502 | 8.95E-52 | -1.69E-46 |
| MS.gene059536 | MS.gene95885 | 0.800197 | 1.61E-48 | -1.69E-46 |
| MS.gene059597 | MS.gene95885 | 0.855943 | 4.49E-62 | -1.69E-46 |
| MS.gene05959 | MS.gene95885 | 0.811332 | 7.38E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene95885 | 0.851106 | 1.10E-60 | -1.69E-46 |
| MS.gene059688 | MS.gene95885 | 0.888405 | 6.08E-73 | -1.69E-46 |
| MS.gene059692 | MS.gene95885 | 0.882284 | 1.19E-70 | -1.69E-46 |
| MS.gene059695 | MS.gene95885 | 0.880742 | 4.27E-70 | -1.69E-46 |
| MS.gene059711 | MS.gene95885 | 0.820992 | 5.12E-53 | -1.69E-46 |
| MS.gene059807 | MS.gene95885 | 0.820419 | 6.93E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene95885 | 0.838499 | 2.77E-57 | -1.69E-46 |
| MS.gene059894 | MS.gene95885 | 0.844753 | 6.22E-59 | -1.69E-46 |
| MS.gene059958 | MS.gene95885 | 0.801323 | 9.51E-49 | -1.69E-46 |
| MS.gene060247 | MS.gene95885 | 0.808102 | 3.65E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene95885 | 0.838786 | 2.34E-57 | -1.69E-46 |
| MS.gene060403 | MS.gene95885 | 0.858124 | 1.02E-62 | -1.69E-46 |
| MS.gene060407 | MS.gene95885 | 0.811008 | 8.68E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene95885 | 0.827367 | 1.63E-54 | -1.69E-46 |
| MS.gene060818 | MS.gene95885 | 0.805477 | 1.31E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene95885 | 0.816833 | 4.51E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene95885 | 0.840037 | 1.11E-57 | -1.69E-46 |
| MS.gene060986 | MS.gene95885 | 0.825857 | 3.73E-54 | -1.69E-46 |
| MS.gene061041 | MS.gene95885 | 0.803585 | 3.25E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene95885 | 0.890757 | 7.38E-74 | -1.69E-46 |
| MS.gene061051 | MS.gene95885 | -0.832637 | 8.44E-56 | -1.69E-46 |
| MS.gene061154 | MS.gene95885 | 0.861541 | 9.55E-64 | -1.69E-46 |
| MS.gene061159 | MS.gene95885 | 0.831398 | 1.71E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95885.t1 | MTR_8g106690 | 98.295 | 176 | 3 | 0 | 1 | 176 | 57 | 232 | 1.65e-126 | 355 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95885.t1 | AT1G18060 | 76.000 | 175 | 41 | 1 | 1 | 174 | 50 | 224 | 1.14e-96 | 279 |
Find 33 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAAATTGTTAGGCAAAA+AGG | 0.263160 | 4.3:+702661 | MS.gene95885:intron |
| ATCGAAAACGGTGTATTTCT+TGG | 0.273425 | 4.3:+702684 | MS.gene95885:CDS |
| GAAGTGGAGGCACTGCATTT+TGG | 0.392068 | 4.3:+702913 | MS.gene95885:CDS |
| GAGAACCTTCGAATAAAAGT+TGG | 0.424151 | 4.3:+702774 | MS.gene95885:CDS |
| ACAAATCAGCCTTGGACAAA+AGG | 0.429622 | 4.3:+702812 | MS.gene95885:CDS |
| GCCTTGGACAAAAGGAAGAC+AGG | 0.435622 | 4.3:+702820 | MS.gene95885:CDS |
| AGGATTCCCCTGGGAGAACC+TGG | 0.442328 | 4.3:+701856 | MS.gene95885:CDS |
| ATCGGACAATTAACATTTGA+AGG | 0.443887 | 4.3:+702711 | MS.gene95885:CDS |
| CAAAAGGAAGACAGGGAACC+AGG | 0.469312 | 4.3:+702828 | MS.gene95885:CDS |
| TCTTGACACCCTTGGAGGAA+AGG | 0.485221 | 4.3:+701836 | MS.gene95885:CDS |
| CAGGGGAATCCTTTCCTCCA+AGG | 0.495511 | 4.3:-701845 | None:intergenic |
| AAACCTCTACAAATCAGCCT+TGG | 0.505462 | 4.3:+702804 | MS.gene95885:CDS |
| CTTGGAGGAAAGGATTCCCC+TGG | 0.519361 | 4.3:+701846 | MS.gene95885:CDS |
| TGTCCAAGGCTGATTTGTAG+AGG | 0.525164 | 4.3:-702807 | None:intergenic |
| GGTGTATTTCTTGGACCTAT+CGG | 0.527087 | 4.3:+702693 | MS.gene95885:CDS |
| AGGGGAATCCTTTCCTCCAA+GGG | 0.528136 | 4.3:-701844 | None:intergenic |
| ATAAGCATCCAGGTTCTCCC+AGG | 0.529448 | 4.3:-701864 | None:intergenic |
| TCAAATGTTAATTGTCCGAT+AGG | 0.532866 | 4.3:-702708 | None:intergenic |
| GCTGAAAGAATCTCTTCTGC+TGG | 0.545166 | 4.3:-701771 | None:intergenic |
| TATCTTTACGTAGGAAGTAG+AGG | 0.562498 | 4.3:+701704 | None:intergenic |
| GTTGCTCGAGGCCGAAGTGG+AGG | 0.570500 | 4.3:+702900 | MS.gene95885:CDS |
| AAATGCAGCTAAGACAAGAA+AGG | 0.570671 | 4.3:+701746 | MS.gene95885:CDS |
| AATCTCTGAACAGCTGTGAG+AGG | 0.593439 | 4.3:-702017 | None:intergenic |
| CCTTGGACAAAAGGAAGACA+GGG | 0.594579 | 4.3:+702821 | MS.gene95885:CDS |
| TAAGCATCCAGGTTCTCCCA+GGG | 0.619857 | 4.3:-701863 | None:intergenic |
| GCTGTTGCTCGAGGCCGAAG+TGG | 0.620105 | 4.3:+702897 | MS.gene95885:CDS |
| AGGGTGTCAAGAAAAGAAGA+AGG | 0.621327 | 4.3:-701825 | None:intergenic |
| AGACAGGGAACCAGGTACAA+AGG | 0.628697 | 4.3:+702836 | MS.gene95885:CDS |
| CATTTGAAGGCAGACTGTCG+TGG | 0.634559 | 4.3:+702724 | MS.gene95885:CDS |
| TTGGAGGAAAGGATTCCCCT+GGG | 0.641606 | 4.3:+701847 | MS.gene95885:CDS |
| AAAATGCAGTGCCTCCACTT+CGG | 0.663787 | 4.3:-702911 | None:intergenic |
| GAGGAAATAGCTGTTGCTCG+AGG | 0.672148 | 4.3:+702888 | MS.gene95885:CDS |
| AAGCATCCAGGTTCTCCCAG+GGG | 0.717216 | 4.3:-701862 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAATTTTTCCACTATAAA+TGG | - | chr4.3:702106-702125 | None:intergenic | 15.0% |
| !!! | GAATTTTAGAAAAAATTAGA+TGG | + | chr4.3:701981-702000 | MS.gene95885:intron | 15.0% |
| !! | ACATAAAACCAGATAAAAAA+TGG | - | chr4.3:702864-702883 | None:intergenic | 20.0% |
| !!! | CTTTAGAAGAAAAAAAACTA+GGG | + | chr4.3:702595-702614 | MS.gene95885:intron | 20.0% |
| !!! | GTTTTTATCTATGAGTTATA+AGG | + | chr4.3:702447-702466 | MS.gene95885:intron | 20.0% |
| !!! | TATCTAGAATGAAATATAGA+TGG | - | chr4.3:702074-702093 | None:intergenic | 20.0% |
| !!! | TCTTTAGAAGAAAAAAAACT+AGG | + | chr4.3:702594-702613 | MS.gene95885:intron | 20.0% |
| !!! | TGTTATGTTGATAAATTGTT+AGG | + | chr4.3:702653-702672 | MS.gene95885:intron | 20.0% |
| !!! | TTTTAGAAAAAATTAGATGG+TGG | + | chr4.3:701984-702003 | MS.gene95885:intron | 20.0% |
| ! | CTTCTAATTATGCAAATGAA+GGG | - | chr4.3:702133-702152 | None:intergenic | 25.0% |
| ! | TATTAAGGACAATTCTACAT+TGG | + | chr4.3:702243-702262 | MS.gene95885:intron | 25.0% |
| ! | TCAAAACACAAACACATAAA+AGG | - | chr4.3:702162-702181 | None:intergenic | 25.0% |
| ! | TGATAAATTGTTAGGCAAAA+AGG | + | chr4.3:702661-702680 | MS.gene95885:intron | 25.0% |
| ! | TGGTAAATCACAATGTTATT+TGG | + | chr4.3:702407-702426 | MS.gene95885:intron | 25.0% |
| !! | AATCACAATGTTATTTGGAA+TGG | + | chr4.3:702412-702431 | MS.gene95885:intron | 25.0% |
| !! | CAATGAAGAATCTTTTATAG+AGG | - | chr4.3:702473-702492 | None:intergenic | 25.0% |
| ACATAAGAGTCCATTTATAG+TGG | + | chr4.3:702093-702112 | MS.gene95885:intron | 30.0% | |
| ATAACATTGTGATTTACCAC+CGG | - | chr4.3:702406-702425 | None:intergenic | 30.0% | |
| ATCGGACAATTAACATTTGA+AGG | + | chr4.3:702711-702730 | MS.gene95885:CDS | 30.0% | |
| GCTTCTAATTATGCAAATGA+AGG | - | chr4.3:702134-702153 | None:intergenic | 30.0% | |
| TATGCCAAATCATCAAACTA+TGG | + | chr4.3:702563-702582 | MS.gene95885:intron | 30.0% | |
| TCAAATGTTAATTGTCCGAT+AGG | - | chr4.3:702711-702730 | None:intergenic | 30.0% | |
| TTCTACATTGGATATTGTGA+TGG | + | chr4.3:702255-702274 | MS.gene95885:intron | 30.0% | |
| TTGCATAAGAAGAGTTAAGA+AGG | - | chr4.3:702496-702515 | None:intergenic | 30.0% | |
| ! | TAGAAAAAATTAGATGGTGG+TGG | + | chr4.3:701987-702006 | MS.gene95885:intron | 30.0% |
| ! | TCTCTTATTTCGTATGATTC+TGG | + | chr4.3:701913-701932 | MS.gene95885:intron | 30.0% |
| ! | TTAGCCATAGTTTGATGATT+TGG | - | chr4.3:702570-702589 | None:intergenic | 30.0% |
| !! | CTGATTTGTAGAGGTTTTAA+AGG | - | chr4.3:702801-702820 | None:intergenic | 30.0% |
| !! | TGTGTTTTGAACTACATTCT+CGG | + | chr4.3:702172-702191 | MS.gene95885:intron | 30.0% |
| !!! | CAAAGGATCCATTTTTTATC+TGG | + | chr4.3:702853-702872 | MS.gene95885:CDS | 30.0% |
| !!! | GATGCTTATTTTTACTGCTA+AGG | + | chr4.3:701878-701897 | MS.gene95885:CDS | 30.0% |
| !!! | TATCTGGTTTTATGTTGATG+AGG | + | chr4.3:702869-702888 | MS.gene95885:CDS | 30.0% |
| AAAAATGGATCCTTTGTACC+TGG | - | chr4.3:702849-702868 | None:intergenic | 35.0% | |
| AAATGCAGCTAAGACAAGAA+AGG | + | chr4.3:701746-701765 | MS.gene95885:CDS | 35.0% | |
| AGCAGTAAAAATAAGCATCC+AGG | - | chr4.3:701877-701896 | None:intergenic | 35.0% | |
| ATCGAAAACGGTGTATTTCT+TGG | + | chr4.3:702684-702703 | MS.gene95885:CDS | 35.0% | |
| CTTAGCCACTTACATGAAAT+CGG | + | chr4.3:702324-702343 | MS.gene95885:intron | 35.0% | |
| GAGAACCTTCGAATAAAAGT+TGG | + | chr4.3:702774-702793 | MS.gene95885:CDS | 35.0% | |
| TAACATTGTGATTTACCACC+GGG | - | chr4.3:702405-702424 | None:intergenic | 35.0% | |
| TAGGCAAAAAGGATCGAAAA+CGG | + | chr4.3:702672-702691 | MS.gene95885:intron | 35.0% | |
| TGGCATATGCAACATTTACA+AGG | - | chr4.3:702550-702569 | None:intergenic | 35.0% | |
| TTAGCCACTTACATGAAATC+GGG | + | chr4.3:702325-702344 | MS.gene95885:intron | 35.0% | |
| ! | AAGGTCCAACTTTTATTCGA+AGG | - | chr4.3:702782-702801 | None:intergenic | 35.0% |
| AAACCTCTACAAATCAGCCT+TGG | + | chr4.3:702804-702823 | MS.gene95885:CDS | 40.0% | |
| AACATTGTGATTTACCACCG+GGG | - | chr4.3:702404-702423 | None:intergenic | 40.0% | |
| ACAAATCAGCCTTGGACAAA+AGG | + | chr4.3:702812-702831 | MS.gene95885:CDS | 40.0% | |
| GGTGTATTTCTTGGACCTAT+CGG | + | chr4.3:702693-702712 | MS.gene95885:CDS | 40.0% | |
| TAACCCCGATTTCATGTAAG+TGG | - | chr4.3:702332-702351 | None:intergenic | 40.0% | |
| TAGCCACTTACATGAAATCG+GGG | + | chr4.3:702326-702345 | MS.gene95885:intron | 40.0% | |
| ! | AGGGTGTCAAGAAAAGAAGA+AGG | - | chr4.3:701828-701847 | None:intergenic | 40.0% |
| ! | GTTATTTGGAATGGCTCTTG+TGG | + | chr4.3:702421-702440 | MS.gene95885:intron | 40.0% |
| ! | TACGAGGTTGTGAGGATTTT+AGG | + | chr4.3:702287-702306 | MS.gene95885:intron | 40.0% |
| ! | TCTTCTTTTCTTGACACCCT+TGG | + | chr4.3:701828-701847 | MS.gene95885:CDS | 40.0% |
| AAAATGCAGTGCCTCCACTT+CGG | - | chr4.3:702914-702933 | None:intergenic | 45.0% | |
| AATCTCTGAACAGCTGTGAG+AGG | - | chr4.3:702020-702039 | None:intergenic | 45.0% | |
| CCTTGGACAAAAGGAAGACA+GGG | + | chr4.3:702821-702840 | MS.gene95885:CDS | 45.0% | |
| GCTGAAAGAATCTCTTCTGC+TGG | - | chr4.3:701774-701793 | None:intergenic | 45.0% | |
| TCCTCACAACCTCGTAAAAG+TGG | - | chr4.3:702283-702302 | None:intergenic | 45.0% | |
| ! | TCTTTTCTTGACACCCTTGG+AGG | + | chr4.3:701831-701850 | MS.gene95885:CDS | 45.0% |
| !! | ACCACTTTTACGAGGTTGTG+AGG | + | chr4.3:702279-702298 | MS.gene95885:intron | 45.0% |
| !! | GTGATGGTACCACTTTTACG+AGG | + | chr4.3:702271-702290 | MS.gene95885:intron | 45.0% |
| !! | TGTCCAAGGCTGATTTGTAG+AGG | - | chr4.3:702810-702829 | None:intergenic | 45.0% |
| !!! | TATAATTTTTACTTATATTA+AGG | + | chr4.3:702228-702247 | MS.gene95885:intron | 5.0% |
| AGACAGGGAACCAGGTACAA+AGG | + | chr4.3:702836-702855 | MS.gene95885:CDS | 50.0% | |
| AGGGGAATCCTTTCCTCCAA+GGG | - | chr4.3:701847-701866 | None:intergenic | 50.0% | |
| ATAAGCATCCAGGTTCTCCC+AGG | - | chr4.3:701867-701886 | None:intergenic | 50.0% | |
| CAAAAGGAAGACAGGGAACC+AGG | + | chr4.3:702828-702847 | MS.gene95885:CDS | 50.0% | |
| CATTTGAAGGCAGACTGTCG+TGG | + | chr4.3:702724-702743 | MS.gene95885:CDS | 50.0% | |
| GAGGAAATAGCTGTTGCTCG+AGG | + | chr4.3:702888-702907 | MS.gene95885:CDS | 50.0% | |
| GCCTTGGACAAAAGGAAGAC+AGG | + | chr4.3:702820-702839 | MS.gene95885:CDS | 50.0% | |
| TAAGCATCCAGGTTCTCCCA+GGG | - | chr4.3:701866-701885 | None:intergenic | 50.0% | |
| TCTTGACACCCTTGGAGGAA+AGG | + | chr4.3:701836-701855 | MS.gene95885:CDS | 50.0% | |
| TTGGAGGAAAGGATTCCCCT+GGG | + | chr4.3:701847-701866 | MS.gene95885:CDS | 50.0% | |
| ! | CCCTGTCTTCCTTTTGTCCA+AGG | - | chr4.3:702824-702843 | None:intergenic | 50.0% |
| !!! | ATTTTGGTGTCGGTGTCGTC+AGG | + | chr4.3:702929-702948 | MS.gene95885:CDS | 50.0% |
| !!! | GAAGTGGAGGCACTGCATTT+TGG | + | chr4.3:702913-702932 | MS.gene95885:CDS | 50.0% |
| !!! | GAGGCACTGCATTTTGGTGT+CGG | + | chr4.3:702919-702938 | MS.gene95885:CDS | 50.0% |
| AAGCATCCAGGTTCTCCCAG+GGG | - | chr4.3:701865-701884 | None:intergenic | 55.0% | |
| CAGGGGAATCCTTTCCTCCA+AGG | - | chr4.3:701848-701867 | None:intergenic | 55.0% | |
| CGACCACTTGTGCTAAACCC+CGG | + | chr4.3:702384-702403 | MS.gene95885:intron | 55.0% | |
| CGGGGTTTAGCACAAGTGGT+CGG | - | chr4.3:702386-702405 | None:intergenic | 55.0% | |
| CTTGGAGGAAAGGATTCCCC+TGG | + | chr4.3:701846-701865 | MS.gene95885:CDS | 55.0% | |
| GGTTTAGCACAAGTGGTCGG+TGG | - | chr4.3:702383-702402 | None:intergenic | 55.0% | |
| AGCACAAGTGGTCGGTGGCT+CGG | - | chr4.3:702378-702397 | None:intergenic | 60.0% | |
| AGGATTCCCCTGGGAGAACC+TGG | + | chr4.3:701856-701875 | MS.gene95885:CDS | 60.0% | |
| CCACCGGGGTTTAGCACAAG+TGG | - | chr4.3:702390-702409 | None:intergenic | 60.0% | |
| CCACTTGTGCTAAACCCCGG+TGG | + | chr4.3:702387-702406 | MS.gene95885:intron | 60.0% | |
| GCTGTTGCTCGAGGCCGAAG+TGG | + | chr4.3:702897-702916 | MS.gene95885:CDS | 65.0% | |
| GTTGCTCGAGGCCGAAGTGG+AGG | + | chr4.3:702900-702919 | MS.gene95885:CDS | 65.0% | |
| !! | GCTCGGTGTGCAGGGTGTGT+GGG | - | chr4.3:702361-702380 | None:intergenic | 65.0% |
| !! | GGCTCGGTGTGCAGGGTGTG+TGG | - | chr4.3:702362-702381 | None:intergenic | 70.0% |
| !! | GGTCGGTGGCTCGGTGTGCA+GGG | - | chr4.3:702369-702388 | None:intergenic | 70.0% |
| !! | TGGTCGGTGGCTCGGTGTGC+AGG | - | chr4.3:702370-702389 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 701717 | 702962 | 701717 | ID=MS.gene95885 |
| chr4.3 | mRNA | 701717 | 702962 | 701717 | ID=MS.gene95885.t1;Parent=MS.gene95885 |
| chr4.3 | exon | 701717 | 701899 | 701717 | ID=MS.gene95885.t1.exon1;Parent=MS.gene95885.t1 |
| chr4.3 | CDS | 701717 | 701899 | 701717 | ID=cds.MS.gene95885.t1;Parent=MS.gene95885.t1 |
| chr4.3 | exon | 701990 | 702049 | 701990 | ID=MS.gene95885.t1.exon2;Parent=MS.gene95885.t1 |
| chr4.3 | CDS | 701990 | 702049 | 701990 | ID=cds.MS.gene95885.t1;Parent=MS.gene95885.t1 |
| chr4.3 | exon | 702675 | 702962 | 702675 | ID=MS.gene95885.t1.exon3;Parent=MS.gene95885.t1 |
| chr4.3 | CDS | 702675 | 702962 | 702675 | ID=cds.MS.gene95885.t1;Parent=MS.gene95885.t1 |
| Gene Sequence |
| Protein sequence |
