AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46239


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46239.t1 MTR_3g088645 100.000 132 0 0 1 132 83 214 6.33e-99 282
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46239.t1 AT4G37925 78.862 123 26 0 4 126 84 206 1.37e-74 221

Find 32 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATCAAAAGCACTGGTTTC+TGG 0.141947 3.1:-71640100 None:intergenic
CTTGGTCTATTCTTACTTTC+AGG 0.183071 3.1:+71640195 MS.gene46239:CDS
CATCAGTAGGATCAAGAATA+AGG 0.246691 3.1:-71640144 None:intergenic
CAAGGCATGACCAGGGAATA+TGG 0.310643 3.1:+71640019 None:intergenic
TAGACCAAGTTGCAAGTATC+AGG 0.336990 3.1:-71640181 None:intergenic
TCTCAAGTACTAGCATCTTC+AGG 0.350319 3.1:-71640901 None:intergenic
GAATACACACTTCGGTTGAT+TGG 0.380394 3.1:+71640751 MS.gene46239:CDS
TTCTTACTTTCAGGAGCTTG+TGG 0.404103 3.1:+71640204 MS.gene46239:CDS
ATAAGAAAGTTGTTATATGA+TGG 0.419220 3.1:+71640793 MS.gene46239:CDS
CGAAGTGTGTATTCATTCAA+TGG 0.452108 3.1:-71640742 None:intergenic
CTGAGTTTGATCAAAAGCAC+TGG 0.490193 3.1:-71640107 None:intergenic
ATCAGTAGGATCAAGAATAA+GGG 0.496505 3.1:-71640143 None:intergenic
TAAAAGATCAAGGCATGACC+AGG 0.521510 3.1:+71640011 None:intergenic
TCAAGTATAATATGAATGCA+AGG 0.530324 3.1:+71640821 MS.gene46239:CDS
CCACTGTCCTCCATATTCCC+TGG 0.567133 3.1:-71640029 None:intergenic
AGGGTTCTGAATTTCAGCAT+GGG 0.568039 3.1:+71640841 MS.gene46239:CDS
CAAGTATAATATGAATGCAA+GGG 0.571561 3.1:+71640822 MS.gene46239:CDS
ATCCTACTGATGAGTTTGTG+TGG 0.588183 3.1:+71640155 MS.gene46239:CDS
AAGGGTTCTGAATTTCAGCA+TGG 0.591866 3.1:+71640840 MS.gene46239:CDS
GAATCCTGATACTTGCAACT+TGG 0.593694 3.1:+71640177 MS.gene46239:CDS
GGAGCTTGTGGATCACTATG+AGG 0.594812 3.1:+71640216 MS.gene46239:CDS
AAGTACTAGCATCTTCAGGT+TGG 0.600680 3.1:-71640897 None:intergenic
CATTGAATGAATACACACTT+CGG 0.613957 3.1:+71640743 MS.gene46239:CDS
GGGTTCTGAATTTCAGCATG+GGG 0.628137 3.1:+71640842 MS.gene46239:CDS
ATTTCAGCATGGGGAAGCCA+CGG 0.628788 3.1:+71640851 MS.gene46239:CDS
TTCCACACAAACTCATCAGT+AGG 0.644633 3.1:-71640157 None:intergenic
TTGTTGTTGAACATGATCCG+TGG 0.663996 3.1:-71640868 None:intergenic
ATAGATCCTCACATGTCGCG+TGG 0.684738 3.1:-71640062 None:intergenic
AAAAGATCAAGGCATGACCA+GGG 0.707335 3.1:+71640012 None:intergenic
CCAGGGAATATGGAGGACAG+TGG 0.721629 3.1:+71640029 MS.gene46239:CDS
GCTGTGCCACGCGACATGTG+AGG 0.722585 3.1:+71640056 MS.gene46239:CDS
GGCATGACCAGGGAATATGG+AGG 0.766490 3.1:+71640022 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAAATATCAATACA+AGG + chr3.1:71640384-71640403 MS.gene46239:intron 10.0%
!! ATATAATTCTAACACTTAAT+TGG - chr3.1:71640691-71640710 None:intergenic 15.0%
!! TGTATATTTGATTATGTTTA+AGG - chr3.1:71640248-71640267 None:intergenic 15.0%
!! ATAAGAAAGTTGTTATATGA+TGG + chr3.1:71640793-71640812 MS.gene46239:CDS 20.0%
!!! CTTTTTGATAATTGAAAACA+GGG + chr3.1:71640715-71640734 MS.gene46239:intron 20.0%
! AAATATCAATACAAGGATAG+AGG + chr3.1:71640391-71640410 MS.gene46239:intron 25.0%
! CAAGTATAATATGAATGCAA+GGG + chr3.1:71640822-71640841 MS.gene46239:CDS 25.0%
! TCAAGTATAATATGAATGCA+AGG + chr3.1:71640821-71640840 MS.gene46239:CDS 25.0%
! TGTCTAAAAGGTAAATAAAG+TGG - chr3.1:71640521-71640540 None:intergenic 25.0%
!! ATAATTTAAGACTAGCATGT+AGG + chr3.1:71640317-71640336 MS.gene46239:intron 25.0%
!! CCTGTTTTCAATTATCAAAA+AGG - chr3.1:71640717-71640736 None:intergenic 25.0%
!!! ACATGCTAGTCTTAAATTAT+AGG - chr3.1:71640317-71640336 None:intergenic 25.0%
!!! CCTTTTTGATAATTGAAAAC+AGG + chr3.1:71640714-71640733 MS.gene46239:intron 25.0%
AGTTGTTAATCCAAAAAGCT+AGG + chr3.1:71640620-71640639 MS.gene46239:intron 30.0%
ATCAGTAGGATCAAGAATAA+GGG - chr3.1:71640146-71640165 None:intergenic 30.0%
CAATTCGAAGTTTGTCTAAA+AGG - chr3.1:71640533-71640552 None:intergenic 30.0%
CATTGAATGAATACACACTT+CGG + chr3.1:71640743-71640762 MS.gene46239:CDS 30.0%
TAATCCAAAAAGCTAGGAAA+AGG + chr3.1:71640626-71640645 MS.gene46239:intron 30.0%
!! TTTTCCTTTTCCTAGCTTTT+TGG - chr3.1:71640633-71640652 None:intergenic 30.0%
AGACAAACTTCGAATTGTCA+AGG + chr3.1:71640536-71640555 MS.gene46239:intron 35.0%
CATCAGTAGGATCAAGAATA+AGG - chr3.1:71640147-71640166 None:intergenic 35.0%
CCGAAAAATTCACCTTTAAC+CGG - chr3.1:71640454-71640473 None:intergenic 35.0%
CGAAGTGTGTATTCATTCAA+TGG - chr3.1:71640745-71640764 None:intergenic 35.0%
CTTGGTCTATTCTTACTTTC+AGG + chr3.1:71640195-71640214 MS.gene46239:CDS 35.0%
! CCGGTTAAAGGTGAATTTTT+CGG + chr3.1:71640451-71640470 MS.gene46239:intron 35.0%
! CTACATATTTTGCTAAGCAG+AGG - chr3.1:71640343-71640362 None:intergenic 35.0%
! TGCTTTTGATCAAACTCAGA+TGG + chr3.1:71640111-71640130 MS.gene46239:CDS 35.0%
AAGTACTAGCATCTTCAGGT+TGG - chr3.1:71640900-71640919 None:intergenic 40.0%
ATCCTACTGATGAGTTTGTG+TGG + chr3.1:71640155-71640174 MS.gene46239:CDS 40.0%
GAAATGATTGAGAGCTCAAG+CGG + chr3.1:71640418-71640437 MS.gene46239:intron 40.0%
GAATACACACTTCGGTTGAT+TGG + chr3.1:71640751-71640770 MS.gene46239:CDS 40.0%
GAATCCTGATACTTGCAACT+TGG + chr3.1:71640177-71640196 MS.gene46239:CDS 40.0%
GACTAAGAATTGTTCTCGTC+AGG - chr3.1:71640495-71640514 None:intergenic 40.0%
TAGACCAAGTTGCAAGTATC+AGG - chr3.1:71640184-71640203 None:intergenic 40.0%
TTCCACACAAACTCATCAGT+AGG - chr3.1:71640160-71640179 None:intergenic 40.0%
TTCTTACTTTCAGGAGCTTG+TGG + chr3.1:71640204-71640223 MS.gene46239:CDS 40.0%
! AGGCTTCATTACTCTGAAAG+CGG + chr3.1:71640556-71640575 MS.gene46239:intron 40.0%
! CTGAGTTTGATCAAAAGCAC+TGG - chr3.1:71640110-71640129 None:intergenic 40.0%
! TTGTTGTTGAACATGATCCG+TGG - chr3.1:71640871-71640890 None:intergenic 40.0%
!! AAGGGTTCTGAATTTCAGCA+TGG + chr3.1:71640840-71640859 MS.gene46239:CDS 40.0%
!! AGGGTTCTGAATTTCAGCAT+GGG + chr3.1:71640841-71640860 MS.gene46239:CDS 40.0%
!! TGATCAAAAGCACTGGTTTC+TGG - chr3.1:71640103-71640122 None:intergenic 40.0%
!! GGGTTCTGAATTTCAGCATG+GGG + chr3.1:71640842-71640861 MS.gene46239:CDS 45.0%
!! GGTTGATGAACTCCGGTTAA+AGG + chr3.1:71640439-71640458 MS.gene46239:intron 45.0%
!! TCTGAAAGCGGATTGTCTCA+AGG + chr3.1:71640568-71640587 MS.gene46239:intron 45.0%
ATAGATCCTCACATGTCGCG+TGG - chr3.1:71640065-71640084 None:intergenic 50.0%
ATTTCAGCATGGGGAAGCCA+CGG + chr3.1:71640851-71640870 MS.gene46239:CDS 50.0%
GGAGCTTGTGGATCACTATG+AGG + chr3.1:71640216-71640235 MS.gene46239:CDS 50.0%
!! CTCAAGCGGTTGATGAACTC+CGG + chr3.1:71640432-71640451 MS.gene46239:intron 50.0%
CCACTGTCCTCCATATTCCC+TGG - chr3.1:71640032-71640051 None:intergenic 55.0%
CCAGGGAATATGGAGGACAG+TGG + chr3.1:71640029-71640048 MS.gene46239:CDS 55.0%
GCTGTGCCACGCGACATGTG+AGG + chr3.1:71640056-71640075 MS.gene46239:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 71640025 71640921 71640025 ID=MS.gene46239
chr3.1 mRNA 71640025 71640921 71640025 ID=MS.gene46239.t1;Parent=MS.gene46239
chr3.1 exon 71640025 71640237 71640025 ID=MS.gene46239.t1.exon1;Parent=MS.gene46239.t1
chr3.1 CDS 71640025 71640237 71640025 ID=cds.MS.gene46239.t1;Parent=MS.gene46239.t1
chr3.1 exon 71640736 71640921 71640736 ID=MS.gene46239.t1.exon2;Parent=MS.gene46239.t1
chr3.1 CDS 71640736 71640921 71640736 ID=cds.MS.gene46239.t1;Parent=MS.gene46239.t1
Gene Sequence

>MS.gene46239

ATGACCAGGGAATATGGAGGACAGTGGTTGAGCTGTGCCACGCGACATGTGAGGATCTATGCAGCTTATATTGATCCAGAAACCAGTGCTTTTGATCAAACTCAGATGGATAAACTCACCCTTATTCTTGATCCTACTGATGAGTTTGTGTGGAATCCTGATACTTGCAACTTGGTCTATTCTTACTTTCAGGAGCTTGTGGATCACTATGAGGTAGTTTCCTTAAACATAATCAAATATACAATAGTTTTTGTCTTAGAATAGTGTCACATATACTGAATTTACATATCCTATAATTTAAGACTAGCATGTAGGCCTCTGCTTAGCAAAATATGTAGAAAAAACATGAAAAGTGATGCAAAAATAAAATATCAATACAAGGATAGAGGAAACGAAATGATTGAGAGCTCAAGCGGTTGATGAACTCCGGTTAAAGGTGAATTTTTCGGAAGTATTCGAGTTCAAATCCTGACGAGAACAATTCTTAGTCAGACCACTTTATTTACCTTTTAGACAAACTTCGAATTGTCAAGGCTTCATTACTCTGAAAGCGGATTGTCTCAAGGCTTAATAATTTAAATTTTGTAACAGTGCTAGTTGTTAATCCAAAAAGCTAGGAAAAGGAAAAATATAATTAGATTTTAGTTATGTGCTAGTTTTGAGCCAATTAAGTGTTAGAATTATATTAACCTTTTTGATAATTGAAAACAGGGTGCTCCATTGAATGAATACACACTTCGGTTGATTGGTTCAGACATAGAGCATTATATAAGAAAGTTGTTATATGATGGAGTAATCAAGTATAATATGAATGCAAGGGTTCTGAATTTCAGCATGGGGAAGCCACGGATCATGTTCAACAACAATGATATCCAACCTGAAGATGCTAGTACTTGA

Protein sequence

>MS.gene46239.t1

MTREYGGQWLSCATRHVRIYAAYIDPETSAFDQTQMDKLTLILDPTDEFVWNPDTCNLVYSYFQELVDHYEGAPLNEYTLRLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDIQPEDAST