AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99806


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99806.t1 MTR_2g082580 97.391 230 6 0 1 230 1 230 1.50e-167 461
MS.gene99806.t1 MTR_5g041910 29.358 109 77 0 104 212 141 249 1.31e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99806.t1 AT3G01440 55.357 224 94 3 1 223 1 219 9.93e-83 246
MS.gene99806.t1 AT4G21280 25.532 235 150 4 1 223 1 222 4.67e-18 80.1
MS.gene99806.t1 AT4G21280 25.424 236 150 5 1 223 1 223 2.76e-17 78.2
MS.gene99806.t1 AT4G05180 25.664 226 158 5 4 223 8 229 7.95e-15 71.6
MS.gene99806.t1 AT4G05180 25.664 226 158 5 4 223 8 229 7.95e-15 71.6
MS.gene99806.t1 AT1G14150 28.346 127 91 0 95 221 61 187 4.84e-13 65.9
MS.gene99806.t1 AT1G14150 30.303 99 69 0 95 193 61 159 3.01e-11 60.5

Find 34 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTAAGGATAATGGATTT+TGG 0.177277 2.3:+14847734 MS.gene99806:CDS
TTTGTTTCTTTAGTTCTTAC+AGG 0.230480 2.3:+14847688 MS.gene99806:CDS
TTAAGGTTCCTTTGTGCATT+TGG 0.247307 2.3:-14847568 None:intergenic
CAAGTAGTAGTTGGATTTCT+TGG 0.273988 2.3:+14847598 MS.gene99806:CDS
AAACTTTACATGGCGAATAT+TGG 0.290067 2.3:+14848614 MS.gene99806:CDS
TTTGGAACAAGGCATGTAAT+TGG 0.297323 2.3:-14847550 None:intergenic
CTATTAGTGACAGTTGGTTC+TGG 0.310416 2.3:-14847772 None:intergenic
TGAAAGTTGACTTGAGTCTT+AGG 0.313305 2.3:-14848736 None:intergenic
GTGACAGTTGGTTCTGGAAT+AGG 0.317646 2.3:-14847766 None:intergenic
ATTTCACTAGCTAAGGATAA+TGG 0.333626 2.3:+14847727 MS.gene99806:CDS
CTACTACTTGTCCTGTCTTA+AGG 0.340805 2.3:-14847585 None:intergenic
TGTAATTGGAGTTAAAGTTT+TGG 0.348907 2.3:-14847536 None:intergenic
GACAGCTTGAAGAAGCTTCA+AGG 0.421927 2.3:+14849154 MS.gene99806:intron
AAGACACACTCAACTATGTT+AGG 0.422431 2.3:+14848708 MS.gene99806:CDS
ATGGCGAATATTGGAGCAAA+AGG 0.440394 2.3:+14848623 MS.gene99806:CDS
GATATTGCGAATGAGAAAAC+GGG 0.451665 2.3:+14848569 MS.gene99806:intron
AATTGCTGCTTATCTTCAGC+AGG 0.453994 2.3:-14848794 None:intergenic
AGATATTGCGAATGAGAAAA+CGG 0.487470 2.3:+14848568 MS.gene99806:intron
ATCTTACTATTAGTGACAGT+TGG 0.488173 2.3:-14847778 None:intergenic
TCCTTTGTGCATTTGGAACA+AGG 0.495526 2.3:-14847561 None:intergenic
AAGACAGGACAAGTAGTAGT+TGG 0.499443 2.3:+14847589 MS.gene99806:CDS
TGCATTTGATCTTCTTGCAA+TGG 0.504301 2.3:+14848670 MS.gene99806:CDS
TGAGAAGATTTCACTAGCTA+AGG 0.517657 2.3:+14847720 MS.gene99806:CDS
TGCTTAAAGATGTCATGGAC+AGG 0.522204 2.3:+14849226 MS.gene99806:CDS
TATTAAGAAGAAACTTTACA+TGG 0.528232 2.3:+14848604 MS.gene99806:CDS
ATTTGATCTTCTTGCAATGG+CGG 0.551344 2.3:+14848673 MS.gene99806:CDS
AGTTATGCTTAAAGATGTCA+TGG 0.580022 2.3:+14849221 MS.gene99806:CDS
GCTCTTCTAACAAACTTGCA+TGG 0.586703 2.3:+14847508 MS.gene99806:CDS
ACACACTCAACTATGTTAGG+AGG 0.598523 2.3:+14848711 MS.gene99806:CDS
GCACAAAGGAACCTTAAGAC+AGG 0.610404 2.3:+14847574 MS.gene99806:CDS
TAAGCAGCAATTGACTGATG+TGG 0.624593 2.3:+14848805 MS.gene99806:CDS
ATTGCTGCTTATCTTCAGCA+GGG 0.656002 2.3:-14848793 None:intergenic
GCCTTGTTCCAAATGCACAA+AGG 0.685170 2.3:+14847560 MS.gene99806:CDS
ATATTGCGAATGAGAAAACG+GGG 0.750300 2.3:+14848570 MS.gene99806:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCTATTATTCAAATTT+AGG - chr2.3:14848410-14848429 None:intergenic 10.0%
!! TTAATAATAAACAAATGTAA+TGG - chr2.3:14848212-14848231 None:intergenic 10.0%
!! AAATATTAATTGTACTATCA+TGG + chr2.3:14848010-14848029 MS.gene99806:intron 15.0%
!! ATTCACATAATATTAAATTG+TGG - chr2.3:14849098-14849117 None:intergenic 15.0%
!! TTAATAATAAACAAATGCAA+TGG + chr2.3:14848123-14848142 MS.gene99806:intron 15.0%
!! TTTGTTTATTATTAAATCGA+TGG + chr2.3:14848217-14848236 MS.gene99806:intron 15.0%
!! TTTGTTTATTATTAAATCGA+TGG - chr2.3:14848236-14848217 None:intergenic 15.0%
!! AATATTATGTGAATAGATGT+TGG + chr2.3:14849104-14849123 MS.gene99806:intron 20.0%
!! TATAATTAACTTCATCATGA+TGG + chr2.3:14848241-14848260 MS.gene99806:intron 20.0%
!! TATAATTAACTTCATCATGA+TGG - chr2.3:14848260-14848241 None:intergenic 20.0%
!! TATAGCTAATGAATTTACAT+TGG + chr2.3:14847811-14847830 MS.gene99806:intron 20.0%
!! TATTAAGAAGAAACTTTACA+TGG + chr2.3:14848604-14848623 MS.gene99806:CDS 20.0%
!! TTTATGTTTCAATTGACATT+GGG + chr2.3:14847902-14847921 MS.gene99806:intron 20.0%
!!! ATTGTTTGACAATTTTGAAA+AGG + chr2.3:14848835-14848854 MS.gene99806:CDS 20.0%
!!! TTTTATGTTTCAATTGACAT+TGG + chr2.3:14847901-14847920 MS.gene99806:intron 20.0%
! ATAATCATCATTCACATTGT+TGG - chr2.3:14848542-14848561 None:intergenic 25.0%
! CAATGTGAATGATGATTATA+TGG + chr2.3:14848543-14848562 MS.gene99806:intron 25.0%
! GTTATGATTGAAATTTAGCA+TGG + chr2.3:14847978-14847997 MS.gene99806:intron 25.0%
!! TGTAATTGGAGTTAAAGTTT+TGG - chr2.3:14847539-14847558 None:intergenic 25.0%
!!! GTATGCTCTTTTTTAAATTG+CGG + chr2.3:14848857-14848876 MS.gene99806:intron 25.0%
!!! TTTGTTTCTTTAGTTCTTAC+AGG + chr2.3:14847688-14847707 MS.gene99806:CDS 25.0%
AAACTTTACATGGCGAATAT+TGG + chr2.3:14848614-14848633 MS.gene99806:CDS 30.0%
AGATATTGCGAATGAGAAAA+CGG + chr2.3:14848568-14848587 MS.gene99806:intron 30.0%
AGTTATGCTTAAAGATGTCA+TGG + chr2.3:14849221-14849240 MS.gene99806:CDS 30.0%
ATCTTACTATTAGTGACAGT+TGG - chr2.3:14847781-14847800 None:intergenic 30.0%
ATTTCACTAGCTAAGGATAA+TGG + chr2.3:14847727-14847746 MS.gene99806:CDS 30.0%
CAAATCTTGTACATAAGCAA+TGG + chr2.3:14848049-14848068 MS.gene99806:intron 30.0%
CATAAAGTTGCAAACAAATG+TGG + chr2.3:14849016-14849035 MS.gene99806:intron 30.0%
TACCTATTAGTGAAGTACAA+TGG + chr2.3:14848164-14848183 MS.gene99806:intron 30.0%
TTAGGGGTAATCTTGTAAAT+TGG + chr2.3:14848350-14848369 MS.gene99806:intron 30.0%
! CAGTATTTTATCACAGAGTT+CGG - chr2.3:14848463-14848482 None:intergenic 30.0%
!! ATCTGTTTACTTTTGAACCA+GGG + chr2.3:14848508-14848527 MS.gene99806:intron 30.0%
!! ATTTTAGTCCTTGATATTGC+AGG + chr2.3:14848894-14848913 MS.gene99806:intron 30.0%
!! CATGTACTTTGATTTTGACA+AGG + chr2.3:14848757-14848776 MS.gene99806:CDS 30.0%
!! TATCTGTTTACTTTTGAACC+AGG + chr2.3:14848507-14848526 MS.gene99806:intron 30.0%
!!! GATAATGGATTTTGGATTGA+TGG + chr2.3:14847742-14847761 MS.gene99806:CDS 30.0%
!!! TAGCTAAGGATAATGGATTT+TGG + chr2.3:14847734-14847753 MS.gene99806:CDS 30.0%
AAGACACACTCAACTATGTT+AGG + chr2.3:14848708-14848727 MS.gene99806:CDS 35.0%
ATATTGCGAATGAGAAAACG+GGG + chr2.3:14848570-14848589 MS.gene99806:CDS 35.0%
ATTTGATCTTCTTGCAATGG+CGG + chr2.3:14848673-14848692 MS.gene99806:CDS 35.0%
CAAAATGTTGCAGACAAATG+TGG + chr2.3:14848943-14848962 MS.gene99806:intron 35.0%
CAAGTAGTAGTTGGATTTCT+TGG + chr2.3:14847598-14847617 MS.gene99806:CDS 35.0%
CTTGATATTGCAGGAAAAAG+TGG + chr2.3:14848903-14848922 MS.gene99806:intron 35.0%
GATATTGCGAATGAGAAAAC+GGG + chr2.3:14848569-14848588 MS.gene99806:intron 35.0%
TGAGAAGATTTCACTAGCTA+AGG + chr2.3:14847720-14847739 MS.gene99806:CDS 35.0%
TGCATTTGATCTTCTTGCAA+TGG + chr2.3:14848670-14848689 MS.gene99806:CDS 35.0%
TGCCATTGTACTTCACTAAT+AGG - chr2.3:14848169-14848188 None:intergenic 35.0%
TTAAGGTTCCTTTGTGCATT+TGG - chr2.3:14847571-14847590 None:intergenic 35.0%
! CACATTTGTCTGCAACATTT+TGG - chr2.3:14848945-14848964 None:intergenic 35.0%
! TTTGGAACAAGGCATGTAAT+TGG - chr2.3:14847553-14847572 None:intergenic 35.0%
!! GACATTGGGACAATTTTGAT+TGG + chr2.3:14847916-14847935 MS.gene99806:intron 35.0%
!! TGAAAGTTGACTTGAGTCTT+AGG - chr2.3:14848739-14848758 None:intergenic 35.0%
!!! CACTTTTTCCTGCAATATCA+AGG - chr2.3:14848905-14848924 None:intergenic 35.0%
!!! GAGCAAAAGGAAGTGTTTTA+AGG + chr2.3:14848636-14848655 MS.gene99806:CDS 35.0%
AAGACAGGACAAGTAGTAGT+TGG + chr2.3:14847589-14847608 MS.gene99806:CDS 40.0%
ACACACTCAACTATGTTAGG+AGG + chr2.3:14848711-14848730 MS.gene99806:CDS 40.0%
AGACCATACTAAGTGGCTTA+GGG + chr2.3:14848333-14848352 MS.gene99806:intron 40.0%
ATGGCGAATATTGGAGCAAA+AGG + chr2.3:14848623-14848642 MS.gene99806:CDS 40.0%
CATAAACATGACCGCAATTG+TGG - chr2.3:14848978-14848997 None:intergenic 40.0%
CTACTACTTGTCCTGTCTTA+AGG - chr2.3:14847588-14847607 None:intergenic 40.0%
GACTTGTGCACTTATCAGTA+AGG - chr2.3:14848300-14848319 None:intergenic 40.0%
GCAAACAAATGTGGCTAATG+CGG + chr2.3:14849025-14849044 MS.gene99806:intron 40.0%
GCTCTTCTAACAAACTTGCA+TGG + chr2.3:14847508-14847527 MS.gene99806:CDS 40.0%
TAAGCAGCAATTGACTGATG+TGG + chr2.3:14848805-14848824 MS.gene99806:CDS 40.0%
TAGACCATACTAAGTGGCTT+AGG + chr2.3:14848332-14848351 MS.gene99806:intron 40.0%
TGCTTAAAGATGTCATGGAC+AGG + chr2.3:14849226-14849245 MS.gene99806:CDS 40.0%
TTGCGGTCATGTTTATGTTG+TGG + chr2.3:14848981-14849000 MS.gene99806:intron 40.0%
! AATTGCTGCTTATCTTCAGC+AGG - chr2.3:14848797-14848816 None:intergenic 40.0%
! ATTGCTGCTTATCTTCAGCA+GGG - chr2.3:14848796-14848815 None:intergenic 40.0%
! CTATTAGTGACAGTTGGTTC+TGG - chr2.3:14847775-14847794 None:intergenic 40.0%
! TCCTTTGTGCATTTGGAACA+AGG - chr2.3:14847564-14847583 None:intergenic 40.0%
AATTGCGGCACAAATGTCGA+TGG - chr2.3:14849064-14849083 None:intergenic 45.0%
ATGTCGATGGACCACAATTG+CGG - chr2.3:14849051-14849070 None:intergenic 45.0%
CATTGTTGGAACAAGAACCC+TGG - chr2.3:14848528-14848547 None:intergenic 45.0%
GACAGCTTGAAGAAGCTTCA+AGG + chr2.3:14849154-14849173 MS.gene99806:intron 45.0%
GACCATACTAAGTGGCTTAG+GGG + chr2.3:14848334-14848353 MS.gene99806:intron 45.0%
GCACAAAGGAACCTTAAGAC+AGG + chr2.3:14847574-14847593 MS.gene99806:CDS 45.0%
GCTGACTAGACCATACTAAG+TGG + chr2.3:14848326-14848345 MS.gene99806:intron 45.0%
GTCAGAGACGTAGACACAAT+TGG + chr2.3:14848437-14848456 MS.gene99806:intron 45.0%
GTGGTCAACAACTGCAATTG+CGG - chr2.3:14849079-14849098 None:intergenic 45.0%
TACCCCTAAGCCACTTAGTA+TGG - chr2.3:14848339-14848358 None:intergenic 45.0%
! GCCTTGTTCCAAATGCACAA+AGG + chr2.3:14847560-14847579 MS.gene99806:CDS 45.0%
!! GTGACAGTTGGTTCTGGAAT+AGG - chr2.3:14847769-14847788 None:intergenic 45.0%
GGCTGATGTGACCACAATTG+CGG + chr2.3:14848964-14848983 MS.gene99806:intron 50.0%
GGCTAATGCGGCCGCAATTG+TGG + chr2.3:14849037-14849056 MS.gene99806:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 14847505 14849275 14847505 ID=MS.gene99806
chr2.3 mRNA 14847505 14849275 14847505 ID=MS.gene99806.t1;Parent=MS.gene99806
chr2.3 exon 14847505 14847793 14847505 ID=MS.gene99806.t1.exon1;Parent=MS.gene99806.t1
chr2.3 CDS 14847505 14847793 14847505 ID=cds.MS.gene99806.t1;Parent=MS.gene99806.t1
chr2.3 exon 14848570 14848856 14848570 ID=MS.gene99806.t1.exon2;Parent=MS.gene99806.t1
chr2.3 CDS 14848570 14848856 14848570 ID=cds.MS.gene99806.t1;Parent=MS.gene99806.t1
chr2.3 exon 14849159 14849275 14849159 ID=MS.gene99806.t1.exon3;Parent=MS.gene99806.t1
chr2.3 CDS 14849159 14849275 14849159 ID=cds.MS.gene99806.t1;Parent=MS.gene99806.t1
Gene Sequence

>MS.gene99806

ATGGCTCTTCTAACAAACTTGCATGGAGTCTCCAAAACTTTAACTCCAATTACATGCCTTGTTCCAAATGCACAAAGGAACCTTAAGACAGGACAAGTAGTAGTTGGATTTCTTGGAAGCAAAACAAAGAAATCATCAGAATGTGAATCAGTTAACACTACAAGAAGAGCAGCTGCAATAAGCTTTGTTTCTTTAGTTCTTACAGGACAATTCAGTGAGAAGATTTCACTAGCTAAGGATAATGGATTTTGGATTGATGGACCTATTCCAGAACCAACTGTCACTAATAGTAAGATTAATTTCTTGTATAGCTAATGAATTTACATTGGACAAAAATAATTACTTATAGTTCTTGCTTACAATACATGCATTTCATTGCTCTTATGTTTGATTGATTTTTATGTTTCAATTGACATTGGGACAATTTTGATTGGTTTTTTGTTCTTTTTTTTTTTTTACTATGTTCTTTTATAGTTATGATTGAAATTTAGCATGGTGAACTTAAAAATATTAATTGTACTATCATGGTGAAGTTAAAAAATAACAAATCTTGTACATAAGCAATGGTGAACACATTTGTTATGAACCATCATGATGAAGTTAATTATAACCATCGATTTAATAATAAACAAATGCAATGGTGAAGATTTGATATGAGTTACCTATTAGTGAAGTACAATGGCAAGAAATATATTAAATCTTGACCATTACATTTGTTTATTATTAAATCGATGGTTATAATTAACTTCATCATGATGGTTCAATCTTTTAAATTCACTATACTAATTGTCACCTTACTGATAAGTGCACAAGTCGCTTGAGCTGACTAGACCATACTAAGTGGCTTAGGGGTAATCTTGTAAATTGGACTATAACATATAAAGTAAATTTGCAAAACTAATCCTAAATTTGAATAATAGAATATCAATGTAGTCAGAGACGTAGACACAATTGGCCGAACTCTGTGATAAAATACTGTGTTTTCTTTGTTTGTGTTTTTCGTATCTGTTTACTTTTGAACCAGGGTTCTTGTTCCAACAATGTGAATGATGATTATATGGTTAGATATTGCGAATGAGAAAACGGGGACACGTTCTTTTATTAAGAAGAAACTTTACATGGCGAATATTGGAGCAAAAGGAAGTGTTTTAAGGATCAAGAAATATGCATTTGATCTTCTTGCAATGGCGGATTTGATTGCACAAGACACACTCAACTATGTTAGGAGGTACCTAAGACTCAAGTCAACTTTCATGTACTTTGATTTTGACAAGGTTATCTCTGCTGTCCCTGCTGAAGATAAGCAGCAATTGACTGATGTGGCAAACAAATTGTTTGACAATTTTGAAAAGGTATGCTCTTTTTTAAATTGCGGTTACGTAGAGTCGCATTTTAGTCCTTGATATTGCAGGAAAAAGTGGTCGATGCGATTGTAGTCCAAAATGTTGCAGACAAATGTGGCTGATGTGACCACAATTGCGGTCATGTTTATGTTGTGGTACTTGATGTTGCATAAAGTTGCAAACAAATGTGGCTAATGCGGCCGCAATTGTGGTCCATCGACATTTGTGCCGCAATTGCAGTTGTTGACCACAATTTAATATTATGTGAATAGATGTTGGATTAATGAATTGAACTTTTTATGCAATGACAGCTTGAAGAAGCTTCAAGGAACAAAAATCTACTTGAAACACAAGCATGCTATAAAGAAACTGAAGTTATGCTTAAAGATGTCATGGACAGGATGACTATAATGTATAAAACAATTTGA

Protein sequence

>MS.gene99806.t1

MALLTNLHGVSKTLTPITCLVPNAQRNLKTGQVVVGFLGSKTKKSSECESVNTTRRAAAISFVSLVLTGQFSEKISLAKDNGFWIDGPIPEPTVTNNIANEKTGTRSFIKKKLYMANIGAKGSVLRIKKYAFDLLAMADLIAQDTLNYVRRYLRLKSTFMYFDFDKVISAVPAEDKQQLTDVANKLFDNFEKLEEASRNKNLLETQACYKETEVMLKDVMDRMTIMYKTI