Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43917.t1 | RHN55120.1 | 89.7 | 253 | 26 | 0 | 1 | 253 | 172 | 424 | 4.20E-116 | 427.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43917.t1 | G7K2V9 | 89.7 | 253 | 26 | 0 | 1 | 253 | 171 | 423 | 3.0e-116 | 427.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene43917 | 0.839796 | 1.28E-57 | -1.69E-46 |
| MS.gene049150 | MS.gene43917 | 0.84932 | 3.49E-60 | -1.69E-46 |
| MS.gene049151 | MS.gene43917 | 0.810037 | 1.41E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene43917 | 0.849813 | 2.54E-60 | -1.69E-46 |
| MS.gene049170 | MS.gene43917 | 0.819826 | 9.47E-53 | -1.69E-46 |
| MS.gene049260 | MS.gene43917 | 0.836517 | 8.94E-57 | -1.69E-46 |
| MS.gene049297 | MS.gene43917 | 0.802617 | 5.15E-49 | -1.69E-46 |
| MS.gene04933 | MS.gene43917 | 0.824905 | 6.27E-54 | -1.69E-46 |
| MS.gene049346 | MS.gene43917 | 0.82592 | 3.60E-54 | -1.69E-46 |
| MS.gene049351 | MS.gene43917 | 0.870248 | 1.68E-66 | -1.69E-46 |
| MS.gene049356 | MS.gene43917 | 0.814021 | 1.90E-51 | -1.69E-46 |
| MS.gene049389 | MS.gene43917 | 0.81025 | 1.27E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene43917 | 0.81836 | 2.04E-52 | -1.69E-46 |
| MS.gene049662 | MS.gene43917 | 0.827973 | 1.16E-54 | -1.69E-46 |
| MS.gene049758 | MS.gene43917 | 0.889723 | 1.88E-73 | -1.69E-46 |
| MS.gene049910 | MS.gene43917 | 0.846223 | 2.49E-59 | -1.69E-46 |
| MS.gene049915 | MS.gene43917 | 0.821497 | 3.91E-53 | -1.69E-46 |
| MS.gene049993 | MS.gene43917 | 0.804538 | 2.06E-49 | -1.69E-46 |
| MS.gene050099 | MS.gene43917 | 0.843727 | 1.17E-58 | -1.69E-46 |
| MS.gene050128 | MS.gene43917 | 0.824922 | 6.21E-54 | -1.69E-46 |
| MS.gene050226 | MS.gene43917 | 0.80836 | 3.22E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene43917 | 0.816803 | 4.58E-52 | -1.69E-46 |
| MS.gene050231 | MS.gene43917 | 0.811424 | 7.05E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene43917 | 0.909391 | 6.00E-82 | -1.69E-46 |
| MS.gene050330 | MS.gene43917 | -0.809138 | 2.19E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene43917 | 0.868683 | 5.44E-66 | -1.69E-46 |
| MS.gene050400 | MS.gene43917 | 0.80694 | 6.45E-50 | -1.69E-46 |
| MS.gene050406 | MS.gene43917 | 0.817291 | 3.56E-52 | -1.69E-46 |
| MS.gene050426 | MS.gene43917 | -0.835299 | 1.82E-56 | -1.69E-46 |
| MS.gene050432 | MS.gene43917 | 0.804799 | 1.82E-49 | -1.69E-46 |
| MS.gene050464 | MS.gene43917 | 0.885211 | 9.88E-72 | -1.69E-46 |
| MS.gene050516 | MS.gene43917 | 0.801974 | 6.99E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene43917 | 0.883577 | 3.99E-71 | -1.69E-46 |
| MS.gene050558 | MS.gene43917 | 0.866457 | 2.81E-65 | -1.69E-46 |
| MS.gene050560 | MS.gene43917 | 0.847822 | 9.07E-60 | -1.69E-46 |
| MS.gene050642 | MS.gene43917 | 0.859498 | 3.97E-63 | -1.69E-46 |
| MS.gene050650 | MS.gene43917 | 0.830612 | 2.66E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene43917 | 0.86854 | 6.05E-66 | -1.69E-46 |
| MS.gene050787 | MS.gene43917 | 0.809143 | 2.19E-50 | -1.69E-46 |
| MS.gene050812 | MS.gene43917 | 0.811266 | 7.63E-51 | -1.69E-46 |
| MS.gene050820 | MS.gene43917 | 0.811501 | 6.78E-51 | -1.69E-46 |
| MS.gene050971 | MS.gene43917 | 0.826754 | 2.28E-54 | -1.69E-46 |
| MS.gene05101 | MS.gene43917 | 0.856836 | 2.46E-62 | -1.69E-46 |
| MS.gene05105 | MS.gene43917 | 0.845545 | 3.80E-59 | -1.69E-46 |
| MS.gene05119 | MS.gene43917 | -0.807286 | 5.45E-50 | -1.69E-46 |
| MS.gene051255 | MS.gene43917 | 0.806193 | 9.27E-50 | -1.69E-46 |
| MS.gene051360 | MS.gene43917 | 0.817957 | 2.52E-52 | -1.69E-46 |
| MS.gene051387 | MS.gene43917 | 0.806406 | 8.35E-50 | -1.69E-46 |
| MS.gene051471 | MS.gene43917 | 0.826699 | 2.35E-54 | -1.69E-46 |
| MS.gene051525 | MS.gene43917 | 0.870158 | 1.80E-66 | -1.69E-46 |
| MS.gene051582 | MS.gene43917 | 0.852942 | 3.31E-61 | -1.69E-46 |
| MS.gene051663 | MS.gene43917 | 0.817584 | 3.06E-52 | -1.69E-46 |
| MS.gene051860 | MS.gene43917 | 0.90324 | 4.26E-79 | -1.69E-46 |
| MS.gene051877 | MS.gene43917 | 0.805621 | 1.22E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene43917 | 0.87052 | 1.37E-66 | -1.69E-46 |
| MS.gene052011 | MS.gene43917 | -0.876529 | 1.30E-68 | -1.69E-46 |
| MS.gene052025 | MS.gene43917 | 0.826508 | 2.61E-54 | -1.69E-46 |
| MS.gene052077 | MS.gene43917 | 0.872204 | 3.81E-67 | -1.69E-46 |
| MS.gene052078 | MS.gene43917 | 0.859629 | 3.62E-63 | -1.69E-46 |
| MS.gene052178 | MS.gene43917 | 0.839878 | 1.22E-57 | -1.69E-46 |
| MS.gene052188 | MS.gene43917 | 0.88988 | 1.63E-73 | -1.69E-46 |
| MS.gene052207 | MS.gene43917 | 0.850065 | 2.16E-60 | -1.69E-46 |
| MS.gene052253 | MS.gene43917 | 0.896106 | 5.07E-76 | -1.69E-46 |
| MS.gene052255 | MS.gene43917 | 0.830917 | 2.24E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene43917 | 0.863056 | 3.27E-64 | -1.69E-46 |
| MS.gene052322 | MS.gene43917 | 0.811843 | 5.71E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene43917 | 0.877949 | 4.17E-69 | -1.69E-46 |
| MS.gene052481 | MS.gene43917 | 0.819087 | 1.40E-52 | -1.69E-46 |
| MS.gene052485 | MS.gene43917 | 0.843025 | 1.81E-58 | -1.69E-46 |
| MS.gene052600 | MS.gene43917 | 0.803591 | 3.24E-49 | -1.69E-46 |
| MS.gene052767 | MS.gene43917 | 0.816194 | 6.27E-52 | -1.69E-46 |
| MS.gene052917 | MS.gene43917 | -0.814306 | 1.65E-51 | -1.69E-46 |
| MS.gene052982 | MS.gene43917 | 0.801731 | 7.84E-49 | -1.69E-46 |
| MS.gene053169 | MS.gene43917 | 0.808136 | 3.59E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene43917 | 0.86583 | 4.44E-65 | -1.69E-46 |
| MS.gene053555 | MS.gene43917 | 0.800119 | 1.67E-48 | -1.69E-46 |
| MS.gene053557 | MS.gene43917 | 0.863078 | 3.22E-64 | -1.69E-46 |
| MS.gene053758 | MS.gene43917 | 0.807623 | 4.62E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene43917 | 0.911107 | 8.84E-83 | -1.69E-46 |
| MS.gene053928 | MS.gene43917 | 0.845739 | 3.37E-59 | -1.69E-46 |
| MS.gene053971 | MS.gene43917 | 0.800701 | 1.27E-48 | -1.69E-46 |
| MS.gene054010 | MS.gene43917 | 0.847896 | 8.65E-60 | -1.69E-46 |
| MS.gene054011 | MS.gene43917 | 0.857523 | 1.54E-62 | -1.69E-46 |
| MS.gene054012 | MS.gene43917 | 0.85234 | 4.92E-61 | -1.69E-46 |
| MS.gene054013 | MS.gene43917 | 0.848344 | 6.51E-60 | -1.69E-46 |
| MS.gene054123 | MS.gene43917 | 0.825697 | 4.07E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene43917 | 0.861419 | 1.04E-63 | -1.69E-46 |
| MS.gene054185 | MS.gene43917 | 0.8047 | 1.90E-49 | -1.69E-46 |
| MS.gene054349 | MS.gene43917 | 0.835634 | 1.50E-56 | -1.69E-46 |
| MS.gene054473 | MS.gene43917 | 0.849548 | 3.01E-60 | -1.69E-46 |
| MS.gene054542 | MS.gene43917 | 0.816826 | 4.53E-52 | -1.69E-46 |
| MS.gene054556 | MS.gene43917 | 0.856116 | 4.00E-62 | -1.69E-46 |
| MS.gene05455 | MS.gene43917 | 0.828321 | 9.59E-55 | -1.69E-46 |
| MS.gene054719 | MS.gene43917 | 0.849335 | 3.45E-60 | -1.69E-46 |
| MS.gene054917 | MS.gene43917 | 0.821207 | 4.56E-53 | -1.69E-46 |
| MS.gene054918 | MS.gene43917 | 0.824225 | 9.06E-54 | -1.69E-46 |
| MS.gene054930 | MS.gene43917 | 0.837673 | 4.53E-57 | -1.69E-46 |
| MS.gene054942 | MS.gene43917 | 0.815128 | 1.08E-51 | -1.69E-46 |
| MS.gene054983 | MS.gene43917 | 0.84796 | 8.31E-60 | -1.69E-46 |
| MS.gene055116 | MS.gene43917 | 0.881093 | 3.20E-70 | -1.69E-46 |
| MS.gene055277 | MS.gene43917 | 0.874661 | 5.67E-68 | -1.69E-46 |
| MS.gene055390 | MS.gene43917 | 0.838926 | 2.15E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene43917 | 0.892703 | 1.24E-74 | -1.69E-46 |
| MS.gene055428 | MS.gene43917 | 0.873883 | 1.04E-67 | -1.69E-46 |
| MS.gene05553 | MS.gene43917 | 0.858163 | 9.94E-63 | -1.69E-46 |
| MS.gene055564 | MS.gene43917 | 0.821615 | 3.67E-53 | -1.69E-46 |
| MS.gene055604 | MS.gene43917 | 0.878617 | 2.43E-69 | -1.69E-46 |
| MS.gene055666 | MS.gene43917 | 0.811089 | 8.34E-51 | -1.69E-46 |
| MS.gene055712 | MS.gene43917 | 0.809903 | 1.50E-50 | -1.69E-46 |
| MS.gene055859 | MS.gene43917 | 0.824085 | 9.77E-54 | -1.69E-46 |
| MS.gene05602 | MS.gene43917 | 0.80017 | 1.63E-48 | -1.69E-46 |
| MS.gene056191 | MS.gene43917 | 0.845945 | 2.96E-59 | -1.69E-46 |
| MS.gene056314 | MS.gene43917 | 0.810386 | 1.18E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene43917 | 0.876788 | 1.06E-68 | -1.69E-46 |
| MS.gene056410 | MS.gene43917 | 0.834402 | 3.06E-56 | -1.69E-46 |
| MS.gene056447 | MS.gene43917 | 0.823929 | 1.06E-53 | -1.69E-46 |
| MS.gene056511 | MS.gene43917 | 0.805664 | 1.20E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene43917 | 0.875207 | 3.70E-68 | -1.69E-46 |
| MS.gene056659 | MS.gene43917 | 0.856139 | 3.94E-62 | -1.69E-46 |
| MS.gene056723 | MS.gene43917 | 0.834317 | 3.21E-56 | -1.69E-46 |
| MS.gene056796 | MS.gene43917 | 0.837119 | 6.27E-57 | -1.69E-46 |
| MS.gene056846 | MS.gene43917 | 0.823423 | 1.40E-53 | -1.69E-46 |
| MS.gene056875 | MS.gene43917 | 0.800216 | 1.60E-48 | -1.69E-46 |
| MS.gene056899 | MS.gene43917 | -0.846521 | 2.06E-59 | -1.69E-46 |
| MS.gene056902 | MS.gene43917 | 0.849111 | 3.99E-60 | -1.69E-46 |
| MS.gene056979 | MS.gene43917 | 0.873286 | 1.65E-67 | -1.69E-46 |
| MS.gene05702 | MS.gene43917 | 0.861789 | 8.02E-64 | -1.69E-46 |
| MS.gene057121 | MS.gene43917 | 0.889708 | 1.90E-73 | -1.69E-46 |
| MS.gene057124 | MS.gene43917 | 0.816354 | 5.78E-52 | -1.69E-46 |
| MS.gene057245 | MS.gene43917 | 0.838874 | 2.22E-57 | -1.69E-46 |
| MS.gene057330 | MS.gene43917 | 0.809521 | 1.82E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene43917 | 0.832968 | 6.98E-56 | -1.69E-46 |
| MS.gene057439 | MS.gene43917 | 0.832411 | 9.60E-56 | -1.69E-46 |
| MS.gene057448 | MS.gene43917 | 0.852717 | 3.84E-61 | -1.69E-46 |
| MS.gene057449 | MS.gene43917 | 0.831927 | 1.26E-55 | -1.69E-46 |
| MS.gene057450 | MS.gene43917 | 0.850092 | 2.12E-60 | -1.69E-46 |
| MS.gene057469 | MS.gene43917 | 0.861844 | 7.71E-64 | -1.69E-46 |
| MS.gene057476 | MS.gene43917 | 0.820185 | 7.84E-53 | -1.69E-46 |
| MS.gene057749 | MS.gene43917 | 0.85293 | 3.34E-61 | -1.69E-46 |
| MS.gene057796 | MS.gene43917 | 0.80488 | 1.75E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene43917 | 0.849032 | 4.20E-60 | -1.69E-46 |
| MS.gene057825 | MS.gene43917 | 0.805272 | 1.45E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene43917 | 0.906172 | 1.97E-80 | -1.69E-46 |
| MS.gene058007 | MS.gene43917 | 0.802593 | 5.21E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene43917 | 0.832642 | 8.41E-56 | -1.69E-46 |
| MS.gene05811 | MS.gene43917 | 0.921123 | 5.35E-88 | -1.69E-46 |
| MS.gene058139 | MS.gene43917 | 0.803122 | 4.05E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene43917 | 0.900328 | 8.18E-78 | -1.69E-46 |
| MS.gene058233 | MS.gene43917 | 0.840417 | 8.81E-58 | -1.69E-46 |
| MS.gene058276 | MS.gene43917 | 0.867544 | 1.27E-65 | -1.69E-46 |
| MS.gene058293 | MS.gene43917 | 0.822343 | 2.49E-53 | -1.69E-46 |
| MS.gene058365 | MS.gene43917 | 0.814102 | 1.83E-51 | -1.69E-46 |
| MS.gene058376 | MS.gene43917 | 0.803794 | 2.94E-49 | -1.69E-46 |
| MS.gene05842 | MS.gene43917 | 0.827766 | 1.30E-54 | -1.69E-46 |
| MS.gene058444 | MS.gene43917 | 0.80196 | 7.04E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene43917 | 0.866482 | 2.76E-65 | -1.69E-46 |
| MS.gene058700 | MS.gene43917 | 0.843344 | 1.48E-58 | -1.69E-46 |
| MS.gene058710 | MS.gene43917 | 0.861072 | 1.33E-63 | -1.69E-46 |
| MS.gene058736 | MS.gene43917 | 0.80076 | 1.24E-48 | -1.69E-46 |
| MS.gene058739 | MS.gene43917 | 0.810709 | 1.01E-50 | -1.69E-46 |
| MS.gene058749 | MS.gene43917 | 0.831938 | 1.26E-55 | -1.69E-46 |
| MS.gene058787 | MS.gene43917 | 0.803577 | 3.26E-49 | -1.69E-46 |
| MS.gene058833 | MS.gene43917 | 0.818622 | 1.78E-52 | -1.69E-46 |
| MS.gene058845 | MS.gene43917 | 0.843185 | 1.64E-58 | -1.69E-46 |
| MS.gene05886 | MS.gene43917 | 0.875843 | 2.24E-68 | -1.69E-46 |
| MS.gene058928 | MS.gene43917 | 0.832582 | 8.70E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene43917 | 0.906488 | 1.41E-80 | -1.69E-46 |
| MS.gene059082 | MS.gene43917 | 0.802413 | 5.68E-49 | -1.69E-46 |
| MS.gene059141 | MS.gene43917 | 0.832077 | 1.16E-55 | -1.69E-46 |
| MS.gene059247 | MS.gene43917 | 0.824962 | 6.07E-54 | -1.69E-46 |
| MS.gene059285 | MS.gene43917 | 0.858888 | 6.05E-63 | -1.69E-46 |
| MS.gene059294 | MS.gene43917 | 0.855058 | 8.14E-62 | -1.69E-46 |
| MS.gene059342 | MS.gene43917 | 0.821853 | 3.24E-53 | -1.69E-46 |
| MS.gene059366 | MS.gene43917 | 0.824615 | 7.33E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene43917 | 0.879495 | 1.19E-69 | -1.69E-46 |
| MS.gene059462 | MS.gene43917 | 0.810819 | 9.53E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene43917 | 0.873365 | 1.56E-67 | -1.69E-46 |
| MS.gene059597 | MS.gene43917 | 0.862664 | 4.32E-64 | -1.69E-46 |
| MS.gene05959 | MS.gene43917 | 0.819965 | 8.80E-53 | -1.69E-46 |
| MS.gene059676 | MS.gene43917 | 0.856792 | 2.53E-62 | -1.69E-46 |
| MS.gene059688 | MS.gene43917 | 0.878669 | 2.33E-69 | -1.69E-46 |
| MS.gene059692 | MS.gene43917 | 0.876127 | 1.79E-68 | -1.69E-46 |
| MS.gene059695 | MS.gene43917 | 0.877809 | 4.67E-69 | -1.69E-46 |
| MS.gene05984 | MS.gene43917 | 0.920815 | 7.93E-88 | -1.69E-46 |
| MS.gene059894 | MS.gene43917 | 0.862566 | 4.63E-64 | -1.69E-46 |
| MS.gene059899 | MS.gene43917 | 0.801566 | 8.48E-49 | -1.69E-46 |
| MS.gene059974 | MS.gene43917 | 0.808888 | 2.48E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene43917 | 0.836008 | 1.20E-56 | -1.69E-46 |
| MS.gene060403 | MS.gene43917 | 0.830856 | 2.32E-55 | -1.69E-46 |
| MS.gene060407 | MS.gene43917 | 0.830432 | 2.95E-55 | -1.69E-46 |
| MS.gene060476 | MS.gene43917 | 0.80034 | 1.51E-48 | -1.69E-46 |
| MS.gene060837 | MS.gene43917 | 0.812765 | 3.59E-51 | -1.69E-46 |
| MS.gene06090 | MS.gene43917 | 0.826508 | 2.61E-54 | -1.69E-46 |
| MS.gene060986 | MS.gene43917 | 0.825744 | 3.97E-54 | -1.69E-46 |
| MS.gene060994 | MS.gene43917 | 0.851869 | 6.70E-61 | -1.69E-46 |
| MS.gene061000 | MS.gene43917 | 0.84586 | 3.12E-59 | -1.69E-46 |
| MS.gene061049 | MS.gene43917 | 0.893525 | 5.80E-75 | -1.69E-46 |
| MS.gene061051 | MS.gene43917 | -0.821538 | 3.83E-53 | -1.69E-46 |
| MS.gene061114 | MS.gene43917 | -0.818129 | 2.30E-52 | -1.69E-46 |
| MS.gene061154 | MS.gene43917 | 0.883234 | 5.33E-71 | -1.69E-46 |
| MS.gene061159 | MS.gene43917 | 0.848967 | 4.37E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGAAAGAGAACGCGAAAA+AGG | 0.306013 | 5.4:-25058882 | MS.gene43917:CDS |
| AATATAGTCCCTGCTGTTAT+CGG | 0.320672 | 5.4:-25059826 | MS.gene43917:CDS |
| TTCAAACGAGGATTCCAATA+TGG | 0.378780 | 5.4:-25059149 | MS.gene43917:CDS |
| CTGGTGCAGGTTAAAGATTA+AGG | 0.388699 | 5.4:-25058741 | MS.gene43917:intron |
| ACAGGACAAACTATTACATT+TGG | 0.404371 | 5.4:-25059286 | MS.gene43917:CDS |
| TGAGACTGAAGTAGATGATT+CGG | 0.416272 | 5.4:-25059101 | MS.gene43917:CDS |
| GAAGGCAGAATCATCCATAT+TGG | 0.419468 | 5.4:+25059135 | None:intergenic |
| GCACAGTCTTCCTCTTCTCC+TGG | 0.426196 | 5.4:-25059226 | MS.gene43917:CDS |
| TCACCAAGCGCACTCGGCTT+CGG | 0.431225 | 5.4:+25059917 | None:intergenic |
| ATGTTCCTGGATCTGCAAAA+AGG | 0.434218 | 5.4:-25058853 | MS.gene43917:intron |
| CTCGTTTGAATCTTCTTCAA+AGG | 0.444522 | 5.4:+25059162 | None:intergenic |
| AAAAGCTGCCCTTGCTTCAA+AGG | 0.445602 | 5.4:-25058678 | MS.gene43917:CDS |
| TTCTTGTTTCCGATAACAGC+AGG | 0.456122 | 5.4:+25059817 | None:intergenic |
| AAGCGCACTCGGCTTCGGAC+TGG | 0.457622 | 5.4:+25059922 | None:intergenic |
| ATGATTCTGCCTTCGATACA+TGG | 0.458850 | 5.4:-25059126 | MS.gene43917:CDS |
| GGGACTATATTTGTTATGCC+TGG | 0.464583 | 5.4:+25059838 | None:intergenic |
| GATGACTTCTTCTCAGCTGT+GGG | 0.467868 | 5.4:+25058947 | None:intergenic |
| CAGGTTAAAGATTAAGGAAA+AGG | 0.472906 | 5.4:-25058735 | MS.gene43917:CDS |
| AGATGATTCGGATGAGCTAC+AGG | 0.478552 | 5.4:-25059089 | MS.gene43917:CDS |
| AGATGACTTCTTCTCAGCTG+TGG | 0.479354 | 5.4:+25058946 | None:intergenic |
| AGTCCGAAGCCGAGTGCGCT+TGG | 0.479878 | 5.4:-25059920 | MS.gene43917:CDS |
| CAAATCACCTCTCTCTAAGC+AGG | 0.501806 | 5.4:-25058981 | MS.gene43917:CDS |
| GTAAATTCACCAAGCGCACT+CGG | 0.528586 | 5.4:+25059911 | None:intergenic |
| AGCATGAAGACGATGCAGTT+AGG | 0.534347 | 5.4:-25059750 | MS.gene43917:intron |
| GTTTGAATCTTCTTCAAAGG+AGG | 0.538215 | 5.4:+25059165 | None:intergenic |
| TTTGAATCTTCTTCAAAGGA+GGG | 0.542082 | 5.4:+25059166 | None:intergenic |
| ATGAGCTACAGGAAAGTGAC+GGG | 0.544165 | 5.4:-25059078 | MS.gene43917:CDS |
| GACAAGGGATCCTGTCTGCA+AGG | 0.554772 | 5.4:-25059794 | MS.gene43917:CDS |
| AAAGCAAAACCCTTGCAGAC+AGG | 0.557225 | 5.4:+25059784 | None:intergenic |
| TCTTCAAAGGAGGGCAAGAG+AGG | 0.560364 | 5.4:+25059175 | None:intergenic |
| CTGTCAACCTGCTTAGAGAG+AGG | 0.566700 | 5.4:+25058974 | None:intergenic |
| CTAACTCTCCTTTGAAGCAA+GGG | 0.576199 | 5.4:+25058670 | None:intergenic |
| TTTGTTTAAACCAGGAGAAG+AGG | 0.577338 | 5.4:+25059216 | None:intergenic |
| ACTAACTCTCCTTTGAAGCA+AGG | 0.584876 | 5.4:+25058669 | None:intergenic |
| CTTTGAAGAAGATTCAAACG+AGG | 0.612905 | 5.4:-25059161 | MS.gene43917:CDS |
| GATGAGCTACAGGAAAGTGA+CGG | 0.627818 | 5.4:-25059079 | MS.gene43917:CDS |
| ACAAGGGATCCTGTCTGCAA+GGG | 0.629959 | 5.4:-25059793 | MS.gene43917:CDS |
| AGTCTCATCCCATGTATCGA+AGG | 0.630276 | 5.4:+25059117 | None:intergenic |
| TCTTGTTTCCGATAACAGCA+GGG | 0.632817 | 5.4:+25059818 | None:intergenic |
| TGATTCTGCCTTCGATACAT+GGG | 0.636318 | 5.4:-25059125 | MS.gene43917:CDS |
| TTATCGGAAACAAGAAGACA+AGG | 0.645166 | 5.4:-25059810 | MS.gene43917:CDS |
| GTTCGCCACTGCACTGACTG+TGG | 0.667428 | 5.4:-25059044 | MS.gene43917:CDS |
| ACTATCCACAGTCAGTGCAG+TGG | 0.670660 | 5.4:+25059039 | None:intergenic |
| AGGTTTGTAGAGATATACAC+AGG | 0.675722 | 5.4:-25059304 | MS.gene43917:CDS |
| TATCGGAAACAAGAAGACAA+GGG | 0.712924 | 5.4:-25059809 | MS.gene43917:CDS |
| TGAGCTACAGGAAAGTGACG+GGG | 0.745988 | 5.4:-25059077 | MS.gene43917:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTATAATAATAGAAAATAA+GGG | - | chr5.4:25058896-25058915 | MS.gene43917:CDS | 10.0% |
| !! | TCTTATAATAATAGAAAATA+AGG | - | chr5.4:25058895-25058914 | MS.gene43917:CDS | 10.0% |
| !!! | AGTAAAATGTTTTAATAACA+TGG | + | chr5.4:25059202-25059221 | None:intergenic | 15.0% |
| !!! | GTAAAATGTTTTAATAACAT+GGG | + | chr5.4:25059201-25059220 | None:intergenic | 15.0% |
| !!! | TATTAAAACATTTTACTTCT+TGG | - | chr5.4:25059205-25059224 | MS.gene43917:CDS | 15.0% |
| !! | TGCATGACATTATTATAATA+TGG | - | chr5.4:25058947-25058966 | MS.gene43917:CDS | 20.0% |
| !!! | TTTACAAGTTCACATTTTAT+CGG | + | chr5.4:25059359-25059378 | None:intergenic | 20.0% |
| ! | ATATGGAAAAAGTTTCACAT+TGG | - | chr5.4:25058964-25058983 | MS.gene43917:CDS | 25.0% |
| ! | GAAAAATCTGTGTAATTTAG+CGG | - | chr5.4:25059058-25059077 | MS.gene43917:CDS | 25.0% |
| !! | CTGTCAATTTTTCTTTTCTT+TGG | + | chr5.4:25058720-25058739 | None:intergenic | 25.0% |
| !!! | ATTTGAAATCAGCTTTTCAA+GGG | - | chr5.4:25058742-25058761 | MS.gene43917:intron | 25.0% |
| !!! | TCGTACTAGATTTTTTCTTA+GGG | + | chr5.4:25059709-25059728 | None:intergenic | 25.0% |
| !!! | TTCGTACTAGATTTTTTCTT+AGG | + | chr5.4:25059710-25059729 | None:intergenic | 25.0% |
| !!! | TTGAGATTTTTGTTTAAACC+AGG | + | chr5.4:25059401-25059420 | None:intergenic | 25.0% |
| ACAGGACAAACTATTACATT+TGG | - | chr5.4:25059320-25059339 | MS.gene43917:intron | 30.0% | |
| CAGGTTAAAGATTAAGGAAA+AGG | - | chr5.4:25059871-25059890 | MS.gene43917:CDS | 30.0% | |
| TTTGAATCTTCTTCAAAGGA+GGG | + | chr5.4:25059443-25059462 | None:intergenic | 30.0% | |
| ! | AAATAAGGGAAGAGTATGTT+TGG | - | chr5.4:25058910-25058929 | MS.gene43917:CDS | 30.0% |
| ! | TTCTCATGTATGATTGATTC+TGG | - | chr5.4:25059846-25059865 | MS.gene43917:CDS | 30.0% |
| !! | GATTTGAAATCAGCTTTTCA+AGG | - | chr5.4:25058741-25058760 | MS.gene43917:intron | 30.0% |
| !!! | AAAAAGGTTTTAGATGTTCC+TGG | - | chr5.4:25059740-25059759 | MS.gene43917:intron | 30.0% |
| !!! | CGTACTAGATTTTTTCTTAG+GGG | + | chr5.4:25059708-25059727 | None:intergenic | 30.0% |
| AATATAGTCCCTGCTGTTAT+CGG | - | chr5.4:25058780-25058799 | MS.gene43917:intron | 35.0% | |
| AGGTTTGTAGAGATATACAC+AGG | - | chr5.4:25059302-25059321 | MS.gene43917:CDS | 35.0% | |
| CTCGTTTGAATCTTCTTCAA+AGG | + | chr5.4:25059447-25059466 | None:intergenic | 35.0% | |
| GTTTGAATCTTCTTCAAAGG+AGG | + | chr5.4:25059444-25059463 | None:intergenic | 35.0% | |
| TATCGGAAACAAGAAGACAA+GGG | - | chr5.4:25058797-25058816 | MS.gene43917:intron | 35.0% | |
| TGAGACTGAAGTAGATGATT+CGG | - | chr5.4:25059505-25059524 | MS.gene43917:intron | 35.0% | |
| TTATCGGAAACAAGAAGACA+AGG | - | chr5.4:25058796-25058815 | MS.gene43917:intron | 35.0% | |
| TTCAAACGAGGATTCCAATA+TGG | - | chr5.4:25059457-25059476 | MS.gene43917:intron | 35.0% | |
| TTTGTTTAAACCAGGAGAAG+AGG | + | chr5.4:25059393-25059412 | None:intergenic | 35.0% | |
| ! | AGTGATGTTTTCTCCAAGTA+CGG | - | chr5.4:25059902-25059921 | MS.gene43917:CDS | 35.0% |
| ! | CTTTGAAGAAGATTCAAACG+AGG | - | chr5.4:25059445-25059464 | MS.gene43917:intron | 35.0% |
| ! | GGAAGATGGTTTTCTATTCT+TGG | - | chr5.4:25059079-25059098 | MS.gene43917:CDS | 35.0% |
| ATGATTCTGCCTTCGATACA+TGG | - | chr5.4:25059480-25059499 | MS.gene43917:intron | 40.0% | |
| ATGTTCCTGGATCTGCAAAA+AGG | - | chr5.4:25059753-25059772 | MS.gene43917:CDS | 40.0% | |
| CTGGTGCAGGTTAAAGATTA+AGG | - | chr5.4:25059865-25059884 | MS.gene43917:CDS | 40.0% | |
| CTGTGTAATTTAGCGGAAGA+TGG | - | chr5.4:25059065-25059084 | MS.gene43917:CDS | 40.0% | |
| GAAGGCAGAATCATCCATAT+TGG | + | chr5.4:25059474-25059493 | None:intergenic | 40.0% | |
| GATTCACTGTGCATCATAGT+TGG | - | chr5.4:25059777-25059796 | MS.gene43917:CDS | 40.0% | |
| TACGAAAGAGAACGCGAAAA+AGG | - | chr5.4:25059724-25059743 | MS.gene43917:intron | 40.0% | |
| TGATTCTGCCTTCGATACAT+GGG | - | chr5.4:25059481-25059500 | MS.gene43917:intron | 40.0% | |
| ! | AATCACCTTTTTGCAGATCC+AGG | + | chr5.4:25059761-25059780 | None:intergenic | 40.0% |
| ! | TCTTGTTTCCGATAACAGCA+GGG | + | chr5.4:25058791-25058810 | None:intergenic | 40.0% |
| ! | TGTATGATTGATTCTGGTGC+AGG | - | chr5.4:25059852-25059871 | MS.gene43917:CDS | 40.0% |
| ! | TTCTTGTTTCCGATAACAGC+AGG | + | chr5.4:25058792-25058811 | None:intergenic | 40.0% |
| !! | CTAACTCTCCTTTGAAGCAA+GGG | + | chr5.4:25059939-25059958 | None:intergenic | 40.0% |
| !! | GGGACTATATTTGTTATGCC+TGG | + | chr5.4:25058771-25058790 | None:intergenic | 40.0% |
| AAAAGCTGCCCTTGCTTCAA+AGG | - | chr5.4:25059928-25059947 | MS.gene43917:CDS | 45.0% | |
| AAAGCAAAACCCTTGCAGAC+AGG | + | chr5.4:25058825-25058844 | None:intergenic | 45.0% | |
| AGATGACTTCTTCTCAGCTG+TGG | + | chr5.4:25059663-25059682 | None:intergenic | 45.0% | |
| AGCATGAAGACGATGCAGTT+AGG | - | chr5.4:25058856-25058875 | MS.gene43917:CDS | 45.0% | |
| AGTCTCATCCCATGTATCGA+AGG | + | chr5.4:25059492-25059511 | None:intergenic | 45.0% | |
| ATGAGCTACAGGAAAGTGAC+GGG | - | chr5.4:25059528-25059547 | MS.gene43917:intron | 45.0% | |
| CAAATCACCTCTCTCTAAGC+AGG | - | chr5.4:25059625-25059644 | MS.gene43917:intron | 45.0% | |
| GAGTGTATGTGTGTAGCATC+TGG | - | chr5.4:25059151-25059170 | MS.gene43917:CDS | 45.0% | |
| GATGACTTCTTCTCAGCTGT+GGG | + | chr5.4:25059662-25059681 | None:intergenic | 45.0% | |
| GATGAGCTACAGGAAAGTGA+CGG | - | chr5.4:25059527-25059546 | MS.gene43917:intron | 45.0% | |
| GTAAATTCACCAAGCGCACT+CGG | + | chr5.4:25058698-25058717 | None:intergenic | 45.0% | |
| TCACTTGCTCTCTCTTCTTC+AGG | - | chr5.4:25059282-25059301 | MS.gene43917:CDS | 45.0% | |
| ! | AGATGATTCGGATGAGCTAC+AGG | - | chr5.4:25059517-25059536 | MS.gene43917:intron | 45.0% |
| !! | CTGCAAGGGTTTTGCTTTTG+TGG | - | chr5.4:25058827-25058846 | MS.gene43917:intron | 45.0% |
| !! | TCAGCTTTTCAAGGGATTCC+AGG | - | chr5.4:25058750-25058769 | MS.gene43917:intron | 45.0% |
| ACAAGGGATCCTGTCTGCAA+GGG | - | chr5.4:25058813-25058832 | MS.gene43917:intron | 50.0% | |
| ACTATCCACAGTCAGTGCAG+TGG | + | chr5.4:25059570-25059589 | None:intergenic | 50.0% | |
| CTGTCAACCTGCTTAGAGAG+AGG | + | chr5.4:25059635-25059654 | None:intergenic | 50.0% | |
| TCTTCAAAGGAGGGCAAGAG+AGG | + | chr5.4:25059434-25059453 | None:intergenic | 50.0% | |
| TGAGCTACAGGAAAGTGACG+GGG | - | chr5.4:25059529-25059548 | MS.gene43917:intron | 50.0% | |
| ! | GGCAGCTTTTGATCCGTACT+TGG | + | chr5.4:25059918-25059937 | None:intergenic | 50.0% |
| GACAAGGGATCCTGTCTGCA+AGG | - | chr5.4:25058812-25058831 | MS.gene43917:intron | 55.0% | |
| GCACAGTCTTCCTCTTCTCC+TGG | - | chr5.4:25059380-25059399 | MS.gene43917:intron | 55.0% | |
| TCACCAAGCGCACTCGGCTT+CGG | + | chr5.4:25058692-25058711 | None:intergenic | 60.0% | |
| !! | GTTCGCCACTGCACTGACTG+TGG | - | chr5.4:25059562-25059581 | MS.gene43917:intron | 60.0% |
| AAGCGCACTCGGCTTCGGAC+TGG | + | chr5.4:25058687-25058706 | None:intergenic | 65.0% | |
| AGTCCGAAGCCGAGTGCGCT+TGG | - | chr5.4:25058686-25058705 | MS.gene43917:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 25058670 | 25059958 | 25058670 | ID=MS.gene43917 |
| chr5.4 | mRNA | 25058670 | 25059958 | 25058670 | ID=MS.gene43917.t1;Parent=MS.gene43917 |
| chr5.4 | exon | 25059751 | 25059958 | 25059751 | ID=MS.gene43917.t1.exon1;Parent=MS.gene43917.t1 |
| chr5.4 | CDS | 25059751 | 25059958 | 25059751 | ID=cds.MS.gene43917.t1;Parent=MS.gene43917.t1 |
| chr5.4 | exon | 25058854 | 25059324 | 25058854 | ID=MS.gene43917.t1.exon2;Parent=MS.gene43917.t1 |
| chr5.4 | CDS | 25058854 | 25059324 | 25058854 | ID=cds.MS.gene43917.t1;Parent=MS.gene43917.t1 |
| chr5.4 | exon | 25058670 | 25058754 | 25058670 | ID=MS.gene43917.t1.exon3;Parent=MS.gene43917.t1 |
| chr5.4 | CDS | 25058670 | 25058754 | 25058670 | ID=cds.MS.gene43917.t1;Parent=MS.gene43917.t1 |
| Gene Sequence |
| Protein sequence |
