AlfalfaGEDB | MS.gene27134

M. sativa cultivar XinJiangDaYe / MS.gene27134

KEGG: -
GO: GO:0003674 GO:0005488 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0030001 GO:0043167 GO:0043169 GO:0046872 GO:0051179 GO:0051234
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27134.t1	XP_013452105.1	100	75	0	0	1	75	1	75	3.50E-27	131

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27134.t1	O03982	52.1	73	33	2	3	74	2	73	4.3e-10	65.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27134.t1	A0A072UAJ9	100.0	75	0	0	1	75	1	75	2.5e-27	131.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene27134	0.838648	2.54E-57	-1.69E-46
MS.gene04919	MS.gene27134	0.821428	4.06E-53	-1.69E-46
MS.gene04920	MS.gene27134	0.842295	2.82E-58	-1.69E-46
MS.gene049254	MS.gene27134	0.806275	8.90E-50	-1.69E-46
MS.gene049283	MS.gene27134	0.872794	2.42E-67	-1.69E-46
MS.gene049293	MS.gene27134	-0.823522	1.32E-53	-1.69E-46
MS.gene049419	MS.gene27134	-0.846837	1.69E-59	-1.69E-46
MS.gene049499	MS.gene27134	0.834395	3.07E-56	-1.69E-46
MS.gene049983	MS.gene27134	0.805887	1.07E-49	-1.69E-46
MS.gene050654	MS.gene27134	0.823313	1.48E-53	-1.69E-46
MS.gene050752	MS.gene27134	0.823236	1.54E-53	-1.69E-46
MS.gene050945	MS.gene27134	0.833243	5.96E-56	-1.69E-46
MS.gene050955	MS.gene27134	0.814735	1.32E-51	-1.69E-46
MS.gene051003	MS.gene27134	0.810744	9.90E-51	-1.69E-46
MS.gene051332	MS.gene27134	0.805534	1.27E-49	-1.69E-46
MS.gene051343	MS.gene27134	0.832392	9.70E-56	-1.69E-46
MS.gene051363	MS.gene27134	0.818115	2.32E-52	-1.69E-46
MS.gene051365	MS.gene27134	0.802564	5.28E-49	-1.69E-46
MS.gene051597	MS.gene27134	0.842868	1.99E-58	-1.69E-46
MS.gene051676	MS.gene27134	0.828587	8.28E-55	-1.69E-46
MS.gene05188	MS.gene27134	0.842157	3.07E-58	-1.69E-46
MS.gene051902	MS.gene27134	0.836906	7.11E-57	-1.69E-46
MS.gene051975	MS.gene27134	0.859159	5.02E-63	-1.69E-46
MS.gene052072	MS.gene27134	0.809828	1.56E-50	-1.69E-46
MS.gene052108	MS.gene27134	0.806849	6.74E-50	-1.69E-46
MS.gene052434	MS.gene27134	0.809216	2.11E-50	-1.69E-46
MS.gene052889	MS.gene27134	0.800816	1.21E-48	-1.69E-46
MS.gene052947	MS.gene27134	0.802752	4.83E-49	-1.69E-46
MS.gene052954	MS.gene27134	0.8191	1.39E-52	-1.69E-46
MS.gene053006	MS.gene27134	0.803837	2.88E-49	-1.69E-46
MS.gene053058	MS.gene27134	0.826559	2.54E-54	-1.69E-46
MS.gene053226	MS.gene27134	0.801144	1.03E-48	-1.69E-46
MS.gene053298	MS.gene27134	0.856692	2.71E-62	-1.69E-46
MS.gene053340	MS.gene27134	0.82341	1.41E-53	-1.69E-46
MS.gene053564	MS.gene27134	0.825618	4.25E-54	-1.69E-46
MS.gene053931	MS.gene27134	0.822546	2.24E-53	-1.69E-46
MS.gene053963	MS.gene27134	0.845558	3.77E-59	-1.69E-46
MS.gene054222	MS.gene27134	0.828326	9.57E-55	-1.69E-46
MS.gene054403	MS.gene27134	0.828998	6.58E-55	-1.69E-46
MS.gene054479	MS.gene27134	0.813028	3.15E-51	-1.69E-46
MS.gene054657	MS.gene27134	0.817425	3.32E-52	-1.69E-46
MS.gene05469	MS.gene27134	0.853586	2.17E-61	-1.69E-46
MS.gene054892	MS.gene27134	0.807075	6.03E-50	-1.69E-46
MS.gene054893	MS.gene27134	0.814921	1.20E-51	-1.69E-46
MS.gene054946	MS.gene27134	-0.804915	1.72E-49	-1.69E-46
MS.gene054957	MS.gene27134	0.839643	1.40E-57	-1.69E-46
MS.gene05517	MS.gene27134	0.817974	2.50E-52	-1.69E-46
MS.gene055513	MS.gene27134	0.803965	2.71E-49	-1.69E-46
MS.gene055603	MS.gene27134	0.81159	6.49E-51	-1.69E-46
MS.gene055841	MS.gene27134	0.842066	3.24E-58	-1.69E-46
MS.gene056045	MS.gene27134	0.816459	5.47E-52	-1.69E-46
MS.gene056068	MS.gene27134	0.822875	1.87E-53	-1.69E-46
MS.gene056215	MS.gene27134	0.815597	8.52E-52	-1.69E-46
MS.gene056403	MS.gene27134	0.834136	3.57E-56	-1.69E-46
MS.gene056509	MS.gene27134	0.807925	3.98E-50	-1.69E-46
MS.gene056605	MS.gene27134	0.840098	1.07E-57	-1.69E-46
MS.gene056699	MS.gene27134	0.813245	2.82E-51	-1.69E-46
MS.gene056776	MS.gene27134	0.812341	4.45E-51	-1.69E-46
MS.gene05689	MS.gene27134	0.832569	8.77E-56	-1.69E-46
MS.gene056986	MS.gene27134	0.862607	4.50E-64	-1.69E-46
MS.gene057102	MS.gene27134	0.839717	1.34E-57	-1.69E-46
MS.gene057155	MS.gene27134	0.813219	2.86E-51	-1.69E-46
MS.gene057208	MS.gene27134	0.851348	9.41E-61	-1.69E-46
MS.gene057622	MS.gene27134	0.806798	6.91E-50	-1.69E-46
MS.gene057705	MS.gene27134	0.810592	1.07E-50	-1.69E-46
MS.gene05781	MS.gene27134	0.822349	2.48E-53	-1.69E-46
MS.gene057890	MS.gene27134	-0.815019	1.15E-51	-1.69E-46
MS.gene057893	MS.gene27134	-0.809012	2.33E-50	-1.69E-46
MS.gene057999	MS.gene27134	0.830951	2.20E-55	-1.69E-46
MS.gene058159	MS.gene27134	0.822674	2.09E-53	-1.69E-46
MS.gene058279	MS.gene27134	0.812941	3.29E-51	-1.69E-46
MS.gene058500	MS.gene27134	0.800146	1.65E-48	-1.69E-46
MS.gene058592	MS.gene27134	0.803372	3.60E-49	-1.69E-46
MS.gene058781	MS.gene27134	0.808728	2.69E-50	-1.69E-46
MS.gene058783	MS.gene27134	0.802294	6.01E-49	-1.69E-46
MS.gene059146	MS.gene27134	0.850556	1.57E-60	-1.69E-46
MS.gene05952	MS.gene27134	0.817693	2.89E-52	-1.69E-46
MS.gene059830	MS.gene27134	0.81448	1.51E-51	-1.69E-46
MS.gene05985	MS.gene27134	-0.853984	1.66E-61	-1.69E-46
MS.gene059872	MS.gene27134	-0.800795	1.22E-48	-1.69E-46
MS.gene059901	MS.gene27134	-0.84153	4.49E-58	-1.69E-46
MS.gene059935	MS.gene27134	0.817281	3.58E-52	-1.69E-46
MS.gene06011	MS.gene27134	0.847986	8.17E-60	-1.69E-46
MS.gene060244	MS.gene27134	0.824763	6.77E-54	-1.69E-46
MS.gene060365	MS.gene27134	0.844004	9.89E-59	-1.69E-46
MS.gene060527	MS.gene27134	0.822792	1.96E-53	-1.69E-46
MS.gene060586	MS.gene27134	0.814954	1.18E-51	-1.69E-46
MS.gene060763	MS.gene27134	0.825138	5.52E-54	-1.69E-46
MS.gene060830	MS.gene27134	0.804789	1.82E-49	-1.69E-46
MS.gene060879	MS.gene27134	0.814543	1.46E-51	-1.69E-46
MS.gene060933	MS.gene27134	0.81513	1.08E-51	-1.69E-46
MS.gene060935	MS.gene27134	0.836068	1.16E-56	-1.69E-46
MS.gene060937	MS.gene27134	0.845168	4.81E-59	-1.69E-46
MS.gene061188	MS.gene27134	0.814996	1.16E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27134.t1	MTR_6g045257	100.000	109	0	0	1	109	1	109	2.88e-63	187
MS.gene27134.t1	MTR_1g100587	87.500	72	9	0	3	74	4	75	2.72e-26	94.0
MS.gene27134.t1	MTR_8g098915	50.704	71	34	1	4	74	3	72	3.74e-17	71.6
MS.gene27134.t1	MTR_7g079110	53.731	67	30	1	4	70	3	68	1.72e-15	68.6
MS.gene27134.t1	MTR_3g434960	47.368	76	39	1	4	79	3	77	2.49e-15	66.6
MS.gene27134.t1	MTR_3g434890	47.368	76	39	1	4	79	3	77	3.06e-15	66.2
MS.gene27134.t1	MTR_7g451210	50.000	74	36	1	4	77	3	75	3.40e-15	66.2
MS.gene27134.t1	MTR_6g049320	48.611	72	36	1	1	72	30	100	2.22e-14	65.1
MS.gene27134.t1	MTR_6g049260	45.333	75	40	1	4	78	3	76	3.72e-14	63.5
MS.gene27134.t1	MTR_1173s0010	46.053	76	40	1	4	79	3	77	4.42e-14	63.2
MS.gene27134.t1	MTR_7g451200	46.053	76	40	1	4	79	3	77	4.62e-14	63.2
MS.gene27134.t1	MTR_6g049280	47.222	72	37	1	1	72	30	100	7.91e-14	63.5
MS.gene27134.t1	MTR_1g099810	49.296	71	35	1	4	74	3	72	8.03e-14	63.2
MS.gene27134.t1	MTR_6g049320	49.275	69	34	1	4	72	3	70	1.04e-13	62.8
MS.gene27134.t1	MTR_1g099800	49.296	71	35	1	4	74	3	72	1.07e-13	62.8
MS.gene27134.t1	MTR_6g051680	46.575	73	38	1	3	75	2	73	1.31e-13	62.0
MS.gene27134.t1	MTR_3g435000	41.975	81	45	1	1	79	1	81	2.48e-13	61.2
MS.gene27134.t1	MTR_6g049280	47.826	69	35	1	4	72	3	70	2.58e-13	61.6
MS.gene27134.t1	MTR_6g051690	43.836	73	40	1	3	75	2	73	1.18e-12	60.1
MS.gene27134.t1	MTR_0795s0020	47.143	70	30	2	3	72	2	64	1.40e-11	57.4
MS.gene27134.t1	MTR_8g098915	49.020	51	26	0	24	74	3	53	2.01e-11	56.2
MS.gene27134.t1	MTR_1g099640	49.315	73	36	1	3	75	2	73	2.79e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27134.t1	AT5G23760	86.667	75	10	0	1	75	1	75	4.72e-28	98.6
MS.gene27134.t1	AT5G23760	84.375	64	10	0	12	75	1	64	1.76e-20	79.0
MS.gene27134.t1	AT1G01490	52.857	70	31	2	4	72	3	71	1.44e-15	68.6
MS.gene27134.t1	AT1G01490	52.857	70	31	2	4	72	3	71	1.44e-15	68.6
MS.gene27134.t1	AT1G01490	50.685	73	34	2	1	72	27	98	1.58e-15	69.3
MS.gene27134.t1	AT5G52740	48.571	70	33	2	3	72	2	68	8.62e-11	55.1

Find 12 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGAAGGTTGTGTTAA+AGG	0.218749	6.2:+50742070	MS.gene27134:intron
GAAGCAGCCGCAGATATTTA+TGG	0.262029	6.2:+50742134	MS.gene27134:CDS
AGAAAAGAAAGAAGAGAAGA+AGG	0.371134	6.2:+50742408	MS.gene27134:CDS
GAGCAAAAGTTGACAGTGAT+TGG	0.373869	6.2:+50742292	MS.gene27134:CDS
GTTGACAGTGATTGGTTCAA+TGG	0.405513	6.2:+50742300	MS.gene27134:CDS
AGAGAAGAAAGAAGAGAAGA+AGG	0.411074	6.2:+50742456	MS.gene27134:CDS
GAAGAAGGAGGAAAAGAAAG+AGG	0.464286	6.2:+50742471	MS.gene27134:CDS
TTCAATTGTAGCAGATGTAA+AGG	0.500702	6.2:+50742270	MS.gene27134:CDS
GAAGAAAGAAGAGAAGAAGG+AGG	0.516884	6.2:+50742459	MS.gene27134:CDS
AAAGTTGATATTGTATCTGT+TGG	0.520457	6.2:+50742361	MS.gene27134:CDS
TTAGCCTCTTCAAATGGCTC+AGG	0.528497	6.2:+50741818	None:intergenic
ATTCTTACCATAAATATCTG+CGG	0.661214	6.2:-50742141	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATTAAATTAAATTAAAT+AGG	-	chr6.2:50741866-50741885	None:intergenic	0.0%
!!	TAAATTAAATTAAATAGGAT+AGG	-	chr6.2:50741861-50741880	None:intergenic	10.0%
!!!	AATGTTATTTAATAAATCAA+AGG	+	chr6.2:50742203-50742222	MS.gene27134:intron	10.0%
!!!	ATTTAATTTAATTTTGTTCA+TGG	+	chr6.2:50741871-50741890	MS.gene27134:intron	10.0%
!!	ATAAGAGAATGAAAAAAAAT+TGG	-	chr6.2:50742031-50742050	None:intergenic	15.0%
!!	AAATATATAGCTAAATCATG+AGG	-	chr6.2:50741937-50741956	None:intergenic	20.0%
!	AAATCATGAGGATTTACATA+AGG	-	chr6.2:50741925-50741944	None:intergenic	25.0%
!	AGAGAAAAAAGAAGAGAAAA+AGG	+	chr6.2:50742432-50742451	MS.gene27134:CDS	25.0%
!	ATTCTTACCATAAATATCTG+CGG	-	chr6.2:50742144-50742163	None:intergenic	25.0%
!	TCATTCTCTTATTCTTTCAT+TGG	+	chr6.2:50742039-50742058	MS.gene27134:intron	25.0%
!!!	AAAGTTGATATTGTATCTGT+TGG	+	chr6.2:50742361-50742380	MS.gene27134:CDS	25.0%
!!!	GTTGTTGATTTTGATGAATA+GGG	+	chr6.2:50742242-50742261	MS.gene27134:intron	25.0%
!!!	TGTTGTTGATTTTGATGAAT+AGG	+	chr6.2:50742241-50742260	MS.gene27134:intron	25.0%
!!!	TTGTTGATTTTGATGAATAG+GGG	+	chr6.2:50742243-50742262	MS.gene27134:intron	25.0%
	AATAGGATAGGATATGAAAG+AGG	-	chr6.2:50741849-50741868	None:intergenic	30.0%
	ACATAAGGAATCTAAGAGAT+AGG	-	chr6.2:50741910-50741929	None:intergenic	30.0%
	AGAAAAGAAAGAAGAGAAGA+AGG	+	chr6.2:50742408-50742427	MS.gene27134:CDS	30.0%
	ATAGGATAGGATATGAAAGA+GGG	-	chr6.2:50741848-50741867	None:intergenic	30.0%
	GTGAAGAAACTGAAAAAAGT+AGG	+	chr6.2:50742337-50742356	MS.gene27134:CDS	30.0%
	TTCAATTGTAGCAGATGTAA+AGG	+	chr6.2:50742270-50742289	MS.gene27134:CDS	30.0%
	AAAGCAGAAGGTTGTGTTAA+AGG	+	chr6.2:50742070-50742089	MS.gene27134:intron	35.0%
	AGAGAAGAAAGAAGAGAAGA+AGG	+	chr6.2:50742456-50742475	MS.gene27134:CDS	35.0%
!	TCTTTCTTCTCTTCTTTTGC+AGG	-	chr6.2:50742388-50742407	None:intergenic	35.0%
!	TTGGATTGATTCAAAGCAGA+AGG	+	chr6.2:50742058-50742077	MS.gene27134:intron	35.0%
	GAAGAAAGAAGAGAAGAAGG+AGG	+	chr6.2:50742459-50742478	MS.gene27134:CDS	40.0%
	GAAGAAGGAGGAAAAGAAAG+AGG	+	chr6.2:50742471-50742490	MS.gene27134:CDS	40.0%
	GAGCAAAAGTTGACAGTGAT+TGG	+	chr6.2:50742292-50742311	MS.gene27134:CDS	40.0%
!	GTTGACAGTGATTGGTTCAA+TGG	+	chr6.2:50742300-50742319	MS.gene27134:CDS	40.0%
	GAAGCAGCCGCAGATATTTA+TGG	+	chr6.2:50742134-50742153	MS.gene27134:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	50741831	50742504	50741831	ID=MS.gene27134
chr6.2	mRNA	50741831	50742504	50741831	ID=MS.gene27134.t1;Parent=MS.gene27134
chr6.2	exon	50741831	50741839	50741831	ID=MS.gene27134.t1.exon1;Parent=MS.gene27134.t1
chr6.2	CDS	50741831	50741839	50741831	ID=cds.MS.gene27134.t1;Parent=MS.gene27134.t1
chr6.2	exon	50742077	50742155	50742077	ID=MS.gene27134.t1.exon2;Parent=MS.gene27134.t1
chr6.2	CDS	50742077	50742155	50742077	ID=cds.MS.gene27134.t1;Parent=MS.gene27134.t1
chr6.2	exon	50742263	50742504	50742263	ID=MS.gene27134.t1.exon3;Parent=MS.gene27134.t1
chr6.2	CDS	50742263	50742504	50742263	ID=cds.MS.gene27134.t1;Parent=MS.gene27134.t1

Gene Sequence

>MS.gene27134

ATGGCTCAGGTATTCCCTCTTTCATATCCTATCCTATTTAATTTAATTTAATTTTGTTCATGGTAAATATTTTCTTCCTATCTCTTAGATTCCTTATGTAAATCCTCATGATTTAGCTATATATTTAATTAAATTTTATGTATGTTTCAAAGAAATAATTTTAAATATTTTATTTACTAATTTTGATTGTTTATATACCAATTTTTTTTCATTCTCTTATTCTTTCATTGGATTGATTCAAAGCAGAAGGTTGTGTTAAAGGTGTTAACTATGACTGATGACAAAACAAAGCAAAAAGCTATAGAAGCAGCCGCAGATATTTATGGTAAGAATTCATATTTTTAATGCAAAGAATTCATATTTATAGAAAAAAATGTTATTTAATAAATCAAAGGCTGATCTATTGAATTTGTTGTTGATTTTGATGAATAGGGGTTGATTCAATTGTAGCAGATGTAAAGGAGCAAAAGTTGACAGTGATTGGTTCAATGGATACAGTGAAAATTGTGAAGAAACTGAAAAAAGTAGGAAAAGTTGATATTGTATCTGTTGGTCCTGCAAAAGAAGAGAAGAAAGAAGAAAAGAAAGAAGAGAAGAAGGAAGAGAAAAAAGAAGAGAAAAAGGAAGAGAAGAAAGAAGAGAAGAAGGAGGAAAAGAAAGAGGAGAAGAAATAA

Protein sequence

>MS.gene27134.t1

MAQKVVLKVLTMTDDKTKQKAIEAAADIYGVDSIVADVKEQKLTVIGSMDTVKIVKKLKKVGKVDIVSVGPAKEEKKEEKKEEKKEEKKEEKKEEKKEEKKEEKKEEKK