AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058336

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058336.t1 MTR_2g031980 94.888 313 16 0 1 313 1 313 0.0 615
MS.gene058336.t1 MTR_8g038220 48.243 313 161 1 1 313 1 312 6.94e-111 324
MS.gene058336.t1 MTR_8g038220 47.923 313 163 0 1 313 1 313 1.57e-110 323
MS.gene058336.t1 MTR_3g018780 45.981 311 168 0 1 311 1 311 2.18e-107 315
MS.gene058336.t1 MTR_3g018790 46.965 313 166 0 1 313 1 313 2.14e-105 310
MS.gene058336.t1 MTR_8g038180 46.945 311 165 0 1 311 1 311 1.59e-103 305
MS.gene058336.t1 MTR_5g063670 46.006 313 168 1 1 312 1 313 2.62e-103 305
MS.gene058336.t1 MTR_8g038210 44.728 313 172 1 1 313 1 312 1.44e-96 288
MS.gene058336.t1 MTR_8g038210 43.131 313 177 1 1 313 1 312 2.24e-93 280
MS.gene058336.t1 MTR_0276s0050 43.564 303 171 0 11 313 12 314 3.16e-89 269
MS.gene058336.t1 MTR_3g018790 44.398 241 134 0 73 313 19 259 2.14e-72 224
MS.gene058336.t1 MTR_8g038150 37.500 304 187 1 11 311 12 315 1.80e-67 214
MS.gene058336.t1 MTR_6g071605 35.463 313 199 3 1 311 1 312 9.32e-66 209
MS.gene058336.t1 MTR_8g107640 35.430 302 193 2 11 311 12 312 2.12e-65 208
MS.gene058336.t1 MTR_6g071615 36.422 313 196 3 1 311 1 312 1.34e-63 204
MS.gene058336.t1 MTR_1g033560 34.211 304 198 2 11 313 12 314 6.56e-61 197
MS.gene058336.t1 MTR_1g112520 35.161 310 197 3 5 311 4 312 9.67e-61 196
MS.gene058336.t1 MTR_8g038170 39.117 317 164 7 14 311 5 311 6.66e-60 194
MS.gene058336.t1 MTR_8g038170 39.117 317 164 7 14 311 5 311 6.66e-60 194
MS.gene058336.t1 MTR_6g071595 34.114 299 195 2 14 311 15 312 3.65e-56 184
MS.gene058336.t1 MTR_3g018920 41.706 211 91 1 1 211 1 179 5.06e-56 179
MS.gene058336.t1 MTR_6g071595 32.636 239 159 2 14 251 15 252 3.02e-41 144
MS.gene058336.t1 MTR_6g028030 26.277 274 194 4 44 309 34 307 2.45e-27 108
MS.gene058336.t1 MTR_3g018925 46.316 95 51 0 219 313 14 108 5.65e-21 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058336.t1 AT5G12380 58.013 312 131 0 1 312 1 312 1.72e-135 387
MS.gene058336.t1 AT5G10230 46.689 302 161 0 12 313 13 314 3.25e-99 295
MS.gene058336.t1 AT5G10220 46.203 316 167 2 1 313 1 316 2.78e-98 292
MS.gene058336.t1 AT1G35720 44.408 304 168 1 11 313 12 315 1.07e-96 288
MS.gene058336.t1 AT5G65020 42.038 314 180 2 1 312 1 314 3.45e-90 272
MS.gene058336.t1 AT5G65020 42.561 289 165 1 25 312 11 299 2.06e-86 261
MS.gene058336.t1 AT2G38760 41.100 309 176 2 11 313 12 320 2.17e-79 244
MS.gene058336.t1 AT1G68090 33.444 302 200 1 11 311 12 313 2.71e-60 195
MS.gene058336.t1 AT2G38750 34.740 308 176 6 21 310 15 315 1.63e-53 178
MS.gene058336.t1 AT2G38750 34.868 304 173 6 25 310 2 298 3.32e-53 176
MS.gene058336.t1 AT1G68090 32.636 239 160 1 11 248 12 250 3.15e-44 152

Find 42 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGACTTTCAATATCTTTA+AGG 0.055725 2.2:-56444848 MS.gene058336:CDS
TTCAATCAATTGATGAAGTA+TGG 0.245555 2.2:+56444936 None:intergenic
TTTCAATTGTAGGGATTATC+AGG 0.253479 2.2:-56444670 MS.gene058336:intron
CATTGTTTCTCTTCATAAAA+TGG 0.285540 2.2:+56444365 None:intergenic
TCGTGCAGAGAAAGATTTAA+AGG 0.292895 2.2:-56444398 MS.gene058336:CDS
TTACCTTGGCCAGATATCTT+TGG 0.294272 2.2:+56444587 None:intergenic
TAGAAATTCTGAAGAGCTTC+TGG 0.299957 2.2:-56445232 MS.gene058336:CDS
ATCTCACGCCTTGAATGATT+TGG 0.303941 2.2:-56444458 MS.gene058336:CDS
CGATGACAGCCAAAGATATC+TGG 0.346203 2.2:-56444596 MS.gene058336:CDS
TCAATAATCACTTTGTAATC+TGG 0.360081 2.2:+56445267 None:intergenic
GTATGGCACAACAGTTAATA+AGG 0.366846 2.2:-56444821 MS.gene058336:intron
AATATCTTTAAGGACTTGTA+TGG 0.373979 2.2:-56444838 MS.gene058336:CDS
GCACCGATGTATTCATCAAT+TGG 0.380431 2.2:+56444643 None:intergenic
ATAGTTTCAGCGTCTTCAAT+TGG 0.393727 2.2:+56445936 None:intergenic
TTCATTTGCCAAATCATTCA+AGG 0.394711 2.2:+56444450 None:intergenic
ATTCATTTCCCAACAATTCA+AGG 0.397289 2.2:+56444287 None:intergenic
AGCTGGATCCATTGTCCAAT+GGG 0.409595 2.2:+56445335 None:intergenic
GAGAGGCAACATCTTCTTCA+AGG 0.414127 2.2:+56445172 None:intergenic
TTATCAAGAAATTTATCATC+AGG 0.460775 2.2:-56445526 MS.gene058336:CDS
TGAACTTTCAGGAAACTTTG+AGG 0.461813 2.2:-56445481 MS.gene058336:intron
ATCCATTGTCCAATGGGAAA+TGG 0.464428 2.2:+56445341 None:intergenic
TCAAAGAAAACTTGTGAGGC+TGG 0.474305 2.2:-56445550 MS.gene058336:CDS
TATTAACGAGGCATTAAAGA+AGG 0.493668 2.2:-56445295 MS.gene058336:CDS
AGTGTAGCATCTCTTTCAGC+TGG 0.500813 2.2:+56445318 None:intergenic
CAGCTGGATCCATTGTCCAA+TGG 0.528071 2.2:+56445334 None:intergenic
CCATTCATGATTCTGTTGCT+AGG 0.535196 2.2:-56444339 MS.gene058336:CDS
ATTCTGTTGCTAGGAAGACA+TGG 0.545887 2.2:-56444330 MS.gene058336:CDS
ATTAGAGAGCCATTTCCCAT+TGG 0.552802 2.2:-56445350 MS.gene058336:intron
CCTAGCAACAGAATCATGAA+TGG 0.562607 2.2:+56444339 None:intergenic
AATATCACCGGTAGTTTGAG+AGG 0.577177 2.2:+56445155 None:intergenic
GATGTTGCCTCTCAAACTAC+CGG 0.590150 2.2:-56445162 MS.gene058336:CDS
ACATACTTTGCGAATATCAC+CGG 0.599648 2.2:+56445143 None:intergenic
AGCCATTTCCCATTGGACAA+TGG 0.600425 2.2:-56445343 MS.gene058336:CDS
TTCTGTTGCTAGGAAGACAT+GGG 0.615900 2.2:-56444329 MS.gene058336:CDS
ACTATCAAGAATGCATGCAA+AGG 0.624132 2.2:-56445918 MS.gene058336:intron
CAGCCAAAGATATCTGGCCA+AGG 0.638343 2.2:-56444590 MS.gene058336:intron
AATCCAATTGATGAATACAT+CGG 0.642826 2.2:-56444646 MS.gene058336:CDS
AAGCTCAAAGAAAACTTGTG+AGG 0.650998 2.2:-56445554 MS.gene058336:CDS
AGATGAGTTTGATGAGAATG+TGG 0.686942 2.2:-56444974 MS.gene058336:CDS
ATAAGCACTTACAAGTACGA+TGG 0.694159 2.2:-56444997 MS.gene058336:CDS
TCTGTTGCTAGGAAGACATG+GGG 0.714554 2.2:-56444328 MS.gene058336:CDS
AGATGCTACACTTATTAACG+AGG 0.731686 2.2:-56445307 MS.gene058336:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGGTTTTATAAATTTTATA+AGG + chr2.2:56444550-56444569 None:intergenic 10.0%
!!! AGGTTTTATAAATTTTATAA+GGG + chr2.2:56444549-56444568 None:intergenic 10.0%
!!! TGAATTTATTAAATTTTTGA+AGG + chr2.2:56445523-56445542 None:intergenic 10.0%
!! AGAAATTATTAAATAGAGAA+TGG + chr2.2:56445719-56445738 None:intergenic 15.0%
!!! AATTTGTTATTTTCAATTGT+AGG - chr2.2:56445574-56445593 MS.gene058336:CDS 15.0%
!!! ATTTCAATGGTATAAATTTT+TGG + chr2.2:56444886-56444905 None:intergenic 15.0%
!!! ATTTGTTATTTTCAATTGTA+GGG - chr2.2:56445575-56445594 MS.gene058336:CDS 15.0%
!!! GGTTTTATAAATTTTATAAG+GGG + chr2.2:56444548-56444567 None:intergenic 15.0%
!! ACTCAAAACATATAGAATAA+TGG + chr2.2:56444846-56444865 None:intergenic 20.0%
!! TTATCAAGAAATTTATCATC+AGG - chr2.2:56444728-56444747 MS.gene058336:intron 20.0%
!!! CTAAAATATTTTCGTAACTT+TGG + chr2.2:56445693-56445712 None:intergenic 20.0%
!!! TATGTATGTTAACTTGATTT+AGG - chr2.2:56445769-56445788 MS.gene058336:intron 20.0%
! AAATTCATGCAACAAAATCA+AGG - chr2.2:56445536-56445555 MS.gene058336:CDS 25.0%
! AATCCAATTGATGAATACAT+CGG - chr2.2:56445608-56445627 MS.gene058336:CDS 25.0%
! TCAATAATCACTTTGTAATC+TGG + chr2.2:56444990-56445009 None:intergenic 25.0%
! TGTGACTTTCAATATCTTTA+AGG - chr2.2:56445406-56445425 MS.gene058336:intron 25.0%
! TTCAATCAATTGATGAAGTA+TGG + chr2.2:56445321-56445340 None:intergenic 25.0%
!! AATATCTTTAAGGACTTGTA+TGG - chr2.2:56445416-56445435 MS.gene058336:intron 25.0%
!! ACTTTTATCCTTGAATTGTT+GGG - chr2.2:56445959-56445978 MS.gene058336:CDS 25.0%
!! CATTGTTTCTCTTCATAAAA+TGG + chr2.2:56445892-56445911 None:intergenic 25.0%
!!! AACAGTTTTGTTATGTTACA+TGG - chr2.2:56445167-56445186 MS.gene058336:CDS 25.0%
AAATGGCTCTCTAATTTCAA+TGG + chr2.2:56444899-56444918 None:intergenic 30.0%
AAGGTAATCAATTCTAATCG+AGG - chr2.2:56444585-56444604 MS.gene058336:intron 30.0%
ATTCATTTCCCAACAATTCA+AGG + chr2.2:56445970-56445989 None:intergenic 30.0%
CAACAAAATCAAGGATATCA+TGG - chr2.2:56445545-56445564 MS.gene058336:CDS 30.0%
CGATTAGAATTGATTACCTT+TGG + chr2.2:56444585-56444604 None:intergenic 30.0%
GAATTGATTACCTTTGGTAT+AGG + chr2.2:56444579-56444598 None:intergenic 30.0%
TTCATTTGCCAAATCATTCA+AGG + chr2.2:56445807-56445826 None:intergenic 30.0%
TTTCAATTGTAGGGATTATC+AGG - chr2.2:56445584-56445603 MS.gene058336:CDS 30.0%
TTTGAACAAGTGCAACAAAA+TGG - chr2.2:56445197-56445216 MS.gene058336:CDS 30.0%
! ATGTGATTTTTATCGTGTAC+AGG - chr2.2:56444627-56444646 MS.gene058336:CDS 30.0%
! GACTTTTATCCTTGAATTGT+TGG - chr2.2:56445958-56445977 MS.gene058336:CDS 30.0%
! GTTTTCTTTGAGCTTCATTT+CGG + chr2.2:56444695-56444714 None:intergenic 30.0%
! TATTAACGAGGCATTAAAGA+AGG - chr2.2:56444959-56444978 MS.gene058336:CDS 30.0%
! TTTTTATCGTGTACAGGATT+CGG - chr2.2:56444633-56444652 MS.gene058336:CDS 30.0%
!! ACTATTTTTCATGCAGTTGT+TGG - chr2.2:56445226-56445245 MS.gene058336:CDS 30.0%
AAGCTCAAAGAAAACTTGTG+AGG - chr2.2:56444700-56444719 MS.gene058336:intron 35.0%
ACATACTTTGCGAATATCAC+CGG + chr2.2:56445114-56445133 None:intergenic 35.0%
ACATATTCATGTGTCATCAG+TGG - chr2.2:56444413-56444432 MS.gene058336:CDS 35.0%
ACTATCAAGAATGCATGCAA+AGG - chr2.2:56444336-56444355 MS.gene058336:CDS 35.0%
AGATGAGTTTGATGAGAATG+TGG - chr2.2:56445280-56445299 MS.gene058336:CDS 35.0%
AGATGCTACACTTATTAACG+AGG - chr2.2:56444947-56444966 MS.gene058336:CDS 35.0%
ATAAGCACTTACAAGTACGA+TGG - chr2.2:56445257-56445276 MS.gene058336:CDS 35.0%
ATAGTTTCAGCGTCTTCAAT+TGG + chr2.2:56444321-56444340 None:intergenic 35.0%
CAGCTAAAAACTGAACTTTC+AGG - chr2.2:56444762-56444781 MS.gene058336:intron 35.0%
CATATTCATGTGTCATCAGT+GGG - chr2.2:56444414-56444433 MS.gene058336:CDS 35.0%
GTATGGCACAACAGTTAATA+AGG - chr2.2:56445433-56445452 MS.gene058336:intron 35.0%
TCGTGCAGAGAAAGATTTAA+AGG - chr2.2:56445856-56445875 MS.gene058336:intron 35.0%
TGAACTTTCAGGAAACTTTG+AGG - chr2.2:56444773-56444792 MS.gene058336:intron 35.0%
TGATTACCTTTGGTATAGGA+GGG + chr2.2:56444575-56444594 None:intergenic 35.0%
TTGATTACCTTTGGTATAGG+AGG + chr2.2:56444576-56444595 None:intergenic 35.0%
TTTCGTAACTTTGGTTACCT+TGG + chr2.2:56445684-56445703 None:intergenic 35.0%
! TAGAAATTCTGAAGAGCTTC+TGG - chr2.2:56445022-56445041 MS.gene058336:intron 35.0%
! TTTTATCGTGTACAGGATTC+GGG - chr2.2:56444634-56444653 MS.gene058336:CDS 35.0%
!! AATTCTAATCGAGGCATTGT+CGG - chr2.2:56444594-56444613 MS.gene058336:CDS 35.0%
AATATCACCGGTAGTTTGAG+AGG + chr2.2:56445102-56445121 None:intergenic 40.0%
ATCCATTGTCCAATGGGAAA+TGG + chr2.2:56444916-56444935 None:intergenic 40.0%
ATCTCACGCCTTGAATGATT+TGG - chr2.2:56445796-56445815 MS.gene058336:intron 40.0%
ATTAGAGAGCCATTTCCCAT+TGG - chr2.2:56444904-56444923 MS.gene058336:CDS 40.0%
ATTCTGTTGCTAGGAAGACA+TGG - chr2.2:56445924-56445943 MS.gene058336:CDS 40.0%
CCATTCATGATTCTGTTGCT+AGG - chr2.2:56445915-56445934 MS.gene058336:intron 40.0%
CCTAGCAACAGAATCATGAA+TGG + chr2.2:56445918-56445937 None:intergenic 40.0%
TCAAAGAAAACTTGTGAGGC+TGG - chr2.2:56444704-56444723 MS.gene058336:intron 40.0%
TTACCTTGGCCAGATATCTT+TGG + chr2.2:56445670-56445689 None:intergenic 40.0%
TTCTGTTGCTAGGAAGACAT+GGG - chr2.2:56445925-56445944 MS.gene058336:CDS 40.0%
! CTCTCTTTGAGAACATGGTA+TGG + chr2.2:56445471-56445490 None:intergenic 40.0%
! GCACCGATGTATTCATCAAT+TGG + chr2.2:56445614-56445633 None:intergenic 40.0%
! TGTCTCTCTCTTTGAGAACA+TGG + chr2.2:56445476-56445495 None:intergenic 40.0%
AGCCATTTCCCATTGGACAA+TGG - chr2.2:56444911-56444930 MS.gene058336:CDS 45.0%
AGCTGGATCCATTGTCCAAT+GGG + chr2.2:56444922-56444941 None:intergenic 45.0%
AGTGTAGCATCTCTTTCAGC+TGG + chr2.2:56444939-56444958 None:intergenic 45.0%
CCTTTACCCTCCTATACCAA+AGG - chr2.2:56444566-56444585 MS.gene058336:intron 45.0%
CCTTTGGTATAGGAGGGTAA+AGG + chr2.2:56444569-56444588 None:intergenic 45.0%
CGATGACAGCCAAAGATATC+TGG - chr2.2:56445658-56445677 MS.gene058336:intron 45.0%
GAGAGGCAACATCTTCTTCA+AGG + chr2.2:56445085-56445104 None:intergenic 45.0%
GATGTTGCCTCTCAAACTAC+CGG - chr2.2:56445092-56445111 MS.gene058336:intron 45.0%
TCTGTTGCTAGGAAGACATG+GGG - chr2.2:56445926-56445945 MS.gene058336:CDS 45.0%
CAGCCAAAGATATCTGGCCA+AGG - chr2.2:56445664-56445683 MS.gene058336:intron 50.0%
CAGCTGGATCCATTGTCCAA+TGG + chr2.2:56444923-56444942 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 56444285 56445991 56444285 ID=MS.gene058336
chr2.2 mRNA 56444285 56445991 56444285 ID=MS.gene058336.t1;Parent=MS.gene058336
chr2.2 exon 56445919 56445991 56445919 ID=MS.gene058336.t1.exon1;Parent=MS.gene058336.t1
chr2.2 CDS 56445919 56445991 56445919 ID=cds.MS.gene058336.t1;Parent=MS.gene058336.t1
chr2.2 exon 56445482 56445627 56445482 ID=MS.gene058336.t1.exon2;Parent=MS.gene058336.t1
chr2.2 CDS 56445482 56445627 56445482 ID=cds.MS.gene058336.t1;Parent=MS.gene058336.t1
chr2.2 exon 56445149 56445367 56445149 ID=MS.gene058336.t1.exon3;Parent=MS.gene058336.t1
chr2.2 CDS 56445149 56445367 56445149 ID=cds.MS.gene058336.t1;Parent=MS.gene058336.t1
chr2.2 exon 56444822 56445034 56444822 ID=MS.gene058336.t1.exon4;Parent=MS.gene058336.t1
chr2.2 CDS 56444822 56445034 56444822 ID=cds.MS.gene058336.t1;Parent=MS.gene058336.t1
chr2.2 exon 56444591 56444680 56444591 ID=MS.gene058336.t1.exon5;Parent=MS.gene058336.t1
chr2.2 CDS 56444591 56444680 56444591 ID=cds.MS.gene058336.t1;Parent=MS.gene058336.t1
chr2.2 exon 56444285 56444485 56444285 ID=MS.gene058336.t1.exon6;Parent=MS.gene058336.t1
chr2.2 CDS 56444285 56444485 56444285 ID=cds.MS.gene058336.t1;Parent=MS.gene058336.t1
Gene Sequence

>MS.gene058336

ATGGCTTCTCTACTTGCTGTAAAAGACTTTTCTCCAATTGAAGACGCTGAAACTATCAAGAATGCATGCAAAGGTTGTCTTTCTTCAATTTTGTTTTGTCAAAGATTTGATGTTTCTAATGATATTTAACATATTCATGTGTCATCAGTGGGTATATCAATATAATATTTTCATTCAATGATTTTCAAAATATGTAATTGTTTATTATCTCTTAAAAAATTATTTCAACATGTTTTTGTTTTTATAAATTTTAGCTAATTCCCCTTATAAAATTTATAAAACCTTTACCCTCCTATACCAAAGGTAATCAATTCTAATCGAGGCATTGTCGGAGTACTAAATATGTGATTTTTATCGTGTACAGGATTCGGGACAAATGAGACTGCGATTATATCTGTACTAGCACACCGAAATGAAGCTCAAAGAAAACTTGTGAGGCTGGCTTATCAAGAAATTTATCATCAGGATCTTATAGAACAGCTAAAAACTGAACTTTCAGGAAACTTTGAGGTAAATATTTGTTACAATTCATAACACTAATGTTTTAAGCATAAGTTACCATTATTCTATATGTTTTGAGTGAATATCATTCTAATTGCCAAAAATTTATACCATTGAAATTAGAGAGCCATTTCCCATTGGACAATGGATCCAGCTGAAAGAGATGCTACACTTATTAACGAGGCATTAAAGAAGGCAACACCAGATTACAAAGTGATTATTGAGATTGTTTGCACTAGAAATTCTGAAGAGCTTCTGGCTTTAAAGCGTTCATATCATTCTCTTTTCAAGCATTGCCTTGAAGAAGATGTTGCCTCTCAAACTACCGGTGATATTCGCAAAGTATGTGACTCTTAAGCATAATTAGATTTTTATAACGAGAACAGTTTTGTTATGTTACATGGACAATGTTTTGAACAAGTGCAACAAAATGGAATGTAACTATTTTTCATGCAGTTGTTGGTTGCAGTTATAAGCACTTACAAGTACGATGGAGATGAGTTTGATGAGAATGTGGCTCACTCTGAAGCAACCATACTTCATCAATTGATTGAAAAGAGAGCTTTTAACGATGATGAAATGATTAGAATTTTAAGCACAAGAAGTAAGAAGCAGCTTTGTGTGACTTTCAATATCTTTAAGGACTTGTATGGCACAACAGTTAATAAGGTAAGATGAGTTTCCATACCATGTTCTCAAAGAGAGAGACACACATCACTGTCTATCAGAAGTTTCCTTCAAAAATTTAATAAATTCATGCAACAAAATCAAGGATATCATGGAATGAAAATTTGTTATTTTCAATTGTAGGGATTATCAGGTAATCCAATTGATGAATACATCGGTGCACTGCGTACTACAGTTCGATGCATCGATGACAGCCAAAGATATCTGGCCAAGGTAACCAAAGTTACGAAAATATTTTAGTAACCATTCTCTATTTAATAATTTCTTTATTCTATAGTTTTTATTAAACATGTGTATATGTATGTTAACTTGATTTAGGTGTTATCTCACGCCTTGAATGATTTGGCAAATGAAGAAAATGCTTTGAGTCGAGTTATAGTCACTCGTGCAGAGAAAGATTTAAAGGAAATTAATGACCATTTTATGAAGAGAAACAATGTCACCATTCATGATTCTGTTGCTAGGAAGACATGGGGAAATTACAAGACTTTTATCCTTGAATTGTTGGGAAATGAATAA
Protein sequence

>MS.gene058336.t1

MASLLAVKDFSPIEDAETIKNACKGFGTNETAIISVLAHRNEAQRKLVRLAYQEIYHQDLIEQLKTELSGNFERAISHWTMDPAERDATLINEALKKATPDYKVIIEIVCTRNSEELLALKRSYHSLFKHCLEEDVASQTTGDIRKLLVAVISTYKYDGDEFDENVAHSEATILHQLIEKRAFNDDEMIRILSTRSKKQLCVTFNIFKDLYGTTVNKGLSGNPIDEYIGALRTTVRCIDDSQRYLAKVLSHALNDLANEENALSRVIVTRAEKDLKEINDHFMKRNNVTIHDSVARKTWGNYKTFILELLGNE