AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030809

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030809.t1 MTR_0276s0050 97.143 315 9 0 1 315 1 315 0.0 622
MS.gene030809.t1 MTR_5g063670 66.032 315 107 0 1 315 1 315 5.03e-154 434
MS.gene030809.t1 MTR_8g038210 51.429 315 151 2 1 315 1 313 2.22e-104 308
MS.gene030809.t1 MTR_3g018790 50.476 315 155 1 1 315 1 314 4.65e-103 304
MS.gene030809.t1 MTR_8g038220 50.962 312 152 1 1 312 1 311 5.84e-103 304
MS.gene030809.t1 MTR_3g018780 49.524 315 157 2 1 314 1 314 3.73e-102 302
MS.gene030809.t1 MTR_8g038220 51.118 313 149 2 1 312 1 310 7.32e-102 301
MS.gene030809.t1 MTR_8g038180 50.641 312 153 1 1 312 1 311 6.44e-100 296
MS.gene030809.t1 MTR_8g038210 49.524 315 157 2 1 315 1 313 3.37e-99 295
MS.gene030809.t1 MTR_2g031980 43.234 303 172 0 12 314 11 313 6.11e-89 268
MS.gene030809.t1 MTR_8g038150 42.857 315 177 1 1 312 1 315 5.07e-82 251
MS.gene030809.t1 MTR_3g018790 49.587 242 122 0 74 315 19 260 1.10e-74 230
MS.gene030809.t1 MTR_8g107640 37.855 317 193 3 1 315 1 315 7.28e-72 225
MS.gene030809.t1 MTR_1g033560 36.076 316 198 3 1 314 1 314 8.58e-67 212
MS.gene030809.t1 MTR_6g071605 35.783 313 199 2 1 312 1 312 1.35e-64 206
MS.gene030809.t1 MTR_6g071615 37.580 314 192 3 1 312 1 312 1.57e-64 206
MS.gene030809.t1 MTR_1g112520 36.624 314 195 3 1 312 1 312 2.83e-62 200
MS.gene030809.t1 MTR_6g071595 36.943 314 194 3 1 312 1 312 5.03e-56 184
MS.gene030809.t1 MTR_3g018920 44.811 212 84 2 1 212 1 179 1.67e-53 173
MS.gene030809.t1 MTR_8g038170 36.792 318 172 8 14 312 4 311 3.59e-50 169
MS.gene030809.t1 MTR_8g038170 36.792 318 172 8 14 312 4 311 3.59e-50 169
MS.gene030809.t1 MTR_6g071595 35.317 252 159 3 1 250 1 250 2.23e-38 137
MS.gene030809.t1 MTR_6g028030 26.277 274 194 5 45 310 34 307 1.07e-22 96.7
MS.gene030809.t1 MTR_3g018925 47.917 96 50 0 220 315 14 109 1.18e-21 88.2
MS.gene030809.t1 MTR_8g038090 43.478 69 38 1 245 312 22 90 4.01e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030809.t1 AT5G65020 71.338 314 89 1 1 313 1 314 4.76e-169 472
MS.gene030809.t1 AT5G10230 70.701 314 92 0 1 314 1 314 2.21e-166 465
MS.gene030809.t1 AT5G10220 68.987 316 96 1 1 314 1 316 3.36e-161 452
MS.gene030809.t1 AT5G65020 72.318 289 79 1 26 313 11 299 4.33e-156 439
MS.gene030809.t1 AT1G35720 66.456 316 105 1 1 315 1 316 1.54e-153 432
MS.gene030809.t1 AT5G12380 51.118 313 152 1 1 313 1 312 4.25e-110 322
MS.gene030809.t1 AT2G38760 42.138 318 178 2 1 312 1 318 1.37e-82 253
MS.gene030809.t1 AT1G68090 37.380 313 195 1 1 312 1 313 2.15e-63 203
MS.gene030809.t1 AT1G68090 37.349 249 151 3 1 244 1 249 1.76e-43 150
MS.gene030809.t1 AT2G38750 35.526 304 171 6 26 311 2 298 2.49e-43 151
MS.gene030809.t1 AT2G38750 35.505 307 173 6 23 311 16 315 3.31e-43 151

Find 64 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTGAGGGCAGCAATTA+AGG 0.189714 4.2:-11150540 MS.gene030809:CDS
AATCACTATAACAATGAGTT+TGG 0.243154 4.2:-11150899 MS.gene030809:CDS
ATCTCCACTTGAACTTCTTA+AGG 0.350377 4.2:-11151526 MS.gene030809:CDS
TCATGAGAAGATTGCGGATA+AGG 0.357945 4.2:-11150990 MS.gene030809:CDS
GATGTTGCCTATCATACTTC+TGG 0.369007 4.2:-11151459 MS.gene030809:CDS
GCAATTGACAAAGACACTTC+TGG 0.392622 4.2:-11150350 MS.gene030809:CDS
AGTTCCTCAAAATATTTCTC+AGG 0.401647 4.2:+11150506 None:intergenic
TTATTGAGGGCAGCAATTAA+GGG 0.414248 4.2:-11150539 MS.gene030809:CDS
ATCACTATAACAATGAGTTT+GGG 0.415323 4.2:-11150898 MS.gene030809:CDS
GCAGGATTTGGAAACTGATT+CGG 0.447953 4.2:-11150579 MS.gene030809:intron
TGTGGGCATACACCGTTATG+AGG 0.458266 4.2:-11151059 MS.gene030809:CDS
TTGTTGTTACAATCCTGATC+AGG 0.458748 4.2:+11150951 None:intergenic
ACAGGATGGGGAACGAATGA+AGG 0.469249 4.2:-11151998 MS.gene030809:intron
GATAGGCAACATCTTCTTCA+AGG 0.470858 4.2:+11151469 None:intergenic
ACAATCATGATGACCTGATC+AGG 0.471756 4.2:-11150964 MS.gene030809:CDS
GCGAATTGCGGAGGAATACC+AGG 0.479165 4.2:-11150399 MS.gene030809:CDS
ATGAATATCTGAAATTATTG+AGG 0.486467 4.2:-11150553 MS.gene030809:CDS
AGCTGTGCTGCTGTGGACAT+TGG 0.493673 4.2:-11151640 MS.gene030809:CDS
GAAAATTCTCCTTGCACTGA+TGG 0.493991 4.2:-11150318 MS.gene030809:CDS
GGGAAGAAACAGTGTTCCTC+TGG 0.498214 4.2:-11150378 MS.gene030809:CDS
GAGGAACACTGTTTCTTCCC+TGG 0.508290 4.2:+11150381 None:intergenic
GAGCTGCATTTCTATGAGCC+AGG 0.520838 4.2:+11151960 None:intergenic
CTTTAAGAAGATCTTCTCCA+TGG 0.522934 4.2:+11151903 None:intergenic
TGAATATCTGAAATTATTGA+GGG 0.538168 4.2:-11150552 MS.gene030809:CDS
TAGGCTGGCTATAAACAAGA+TGG 0.541067 4.2:-11150480 MS.gene030809:CDS
AAAATTCTCCTTGCACTGAT+GGG 0.542533 4.2:-11150317 MS.gene030809:CDS
AACAGAAAGCTGTGCTGCTG+TGG 0.544066 4.2:-11151647 MS.gene030809:intron
AATTGTTCACTGTCTTCTGA+TGG 0.548405 4.2:+11152539 None:intergenic
AAGTTCAAGTGGAGATCTTG+TGG 0.549416 4.2:+11151533 None:intergenic
GATGGAGAAGGAACGTGAGA+GGG 0.549677 4.2:+11152557 None:intergenic
TCTTGTTTATAGCCAGCCTA+AGG 0.557307 4.2:+11150483 None:intergenic
AGGCTTGATAATATCGATCC+TGG 0.557589 4.2:-11151978 MS.gene030809:CDS
GTTCCTCAAAATATTTCTCA+GGG 0.565806 4.2:+11150507 None:intergenic
GCTGCTGTGGACATTGGATC+CGG 0.567043 4.2:-11151634 MS.gene030809:CDS
GGGTCTGCGTGTAAGTTTCG+CGG 0.567457 4.2:+11151924 None:intergenic
GATGTCACCAGAAGTATGAT+AGG 0.569427 4.2:+11151452 None:intergenic
GGTTGATTTGCAGCGAATTG+CGG 0.585356 4.2:-11150411 MS.gene030809:CDS
AAGTGTCTTTGTCAATTGCA+CGG 0.589714 4.2:+11150354 None:intergenic
TTTAAGAAGATCTTCTCCAT+GGG 0.594102 4.2:+11151904 None:intergenic
TTGTCAATTGCACGGTCCAG+AGG 0.595483 4.2:+11150362 None:intergenic
TCACCTCATCTCCCTCATAA+CGG 0.595788 4.2:+11151047 None:intergenic
TGATGGAGAAGGAACGTGAG+AGG 0.596195 4.2:+11152556 None:intergenic
ATAGGCAACATCTTCTTCAA+GGG 0.598603 4.2:+11151470 None:intergenic
TGATTTGCAGCGAATTGCGG+AGG 0.598869 4.2:-11150408 MS.gene030809:CDS
TGAAAATGCTCTTACTAGAG+TGG 0.606160 4.2:-11150450 MS.gene030809:CDS
CATCATGTCCCATCAGTGCA+AGG 0.606991 4.2:+11150309 None:intergenic
ATTGCTTCATGAGAAGATTG+CGG 0.611914 4.2:-11150996 MS.gene030809:CDS
GTGGGCATACACCGTTATGA+GGG 0.616438 4.2:-11151058 MS.gene030809:CDS
GAAACTTACACGCAGACCCA+TGG 0.623172 4.2:-11151920 MS.gene030809:CDS
AGGCTGGCTATAAACAAGAT+GGG 0.624496 4.2:-11150479 MS.gene030809:CDS
AGATGAGGTGAACATGACAT+TGG 0.629033 4.2:-11151035 MS.gene030809:CDS
TAACGGTGTATGCCCACAAG+AGG 0.631350 4.2:+11151064 None:intergenic
CTCAAAATATTTCTCAGGGT+AGG 0.640542 4.2:+11150511 None:intergenic
TCACTGTCTTCTGATGGAGA+AGG 0.641986 4.2:+11152545 None:intergenic
ATGGAGAAGGAACGTGAGAG+GGG 0.642321 4.2:+11152558 None:intergenic
TTTGCCTTAAGAAGTTCAAG+TGG 0.642700 4.2:+11151522 None:intergenic
ACACCGTTATGAGGGAGATG+AGG 0.647772 4.2:-11151050 MS.gene030809:CDS
TTCAAACAATTCGATCATCG+TGG 0.667000 4.2:-11151565 MS.gene030809:CDS
TACTTCTGGTGACATCCGCA+AGG 0.668178 4.2:-11151445 MS.gene030809:intron
AGTGGTGACAACTAGAGCTG+AGG 0.671413 4.2:-11150432 MS.gene030809:CDS
GTTTGGGAATGTAATAGACA+AGG 0.682197 4.2:-11150882 MS.gene030809:intron
TGATCAGGATTGTAACAACA+AGG 0.686508 4.2:-11150949 MS.gene030809:CDS
CGAATTGCGGAGGAATACCA+GGG 0.703677 4.2:-11150398 MS.gene030809:CDS
GAAGACAGTGAACAATTGCG+CGG 0.707759 4.2:-11152533 MS.gene030809:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTTAAAATTTTGATATA+TGG - chr4.2:11151033-11151052 MS.gene030809:CDS 10.0%
!!! ATTAACTTCCTTTTAATTAA+AGG - chr4.2:11152179-11152198 MS.gene030809:intron 15.0%
!!! TTAACTTCCTTTTAATTAAA+GGG - chr4.2:11152180-11152199 MS.gene030809:intron 15.0%
!! AAAGGTTAAATACATATAAC+TGG + chr4.2:11151703-11151722 None:intergenic 20.0%
!! ACAGAAATAAGAATCATAAA+TGG + chr4.2:11152037-11152056 None:intergenic 20.0%
!! ATATTTCTTGAAGAGAAAAA+TGG + chr4.2:11152125-11152144 None:intergenic 20.0%
!! CAAGAAATTAGTAAGTTTAA+GGG + chr4.2:11151464-11151483 None:intergenic 20.0%
!! CTAATATCTTAATTAGATCA+TGG - chr4.2:11150497-11150516 MS.gene030809:CDS 20.0%
!! CTATAATCCCTTTAATTAAA+AGG + chr4.2:11152190-11152209 None:intergenic 20.0%
!! CTTCATCAATAATATGATTA+TGG + chr4.2:11152157-11152176 None:intergenic 20.0%
!! TCAAGAAATTAGTAAGTTTA+AGG + chr4.2:11151465-11151484 None:intergenic 20.0%
!! TGAAATAAAAGTCAAAGAAA+TGG + chr4.2:11150399-11150418 None:intergenic 20.0%
!!! ATATTTCTAGTTAATGAATG+TGG - chr4.2:11150609-11150628 MS.gene030809:intron 20.0%
!!! ATGAATATCTGAAATTATTG+AGG - chr4.2:11152326-11152345 MS.gene030809:intron 20.0%
!!! TGAATATCTGAAATTATTGA+GGG - chr4.2:11152327-11152346 MS.gene030809:intron 20.0%
!!! TGTAAATCTTTTCAACTTTA+AGG - chr4.2:11151584-11151603 MS.gene030809:CDS 20.0%
! AAAATCTTCCAAAATTTACG+TGG - chr4.2:11151561-11151580 MS.gene030809:CDS 25.0%
! AAACAAAATTTCATCAGACA+AGG + chr4.2:11151769-11151788 None:intergenic 25.0%
! AACAAAATTTCATCAGACAA+GGG + chr4.2:11151768-11151787 None:intergenic 25.0%
! AATCACTATAACAATGAGTT+TGG - chr4.2:11151980-11151999 MS.gene030809:CDS 25.0%
! ATCAATAATATGATTATGGC+TGG + chr4.2:11152153-11152172 None:intergenic 25.0%
! ATCACTATAACAATGAGTTT+GGG - chr4.2:11151981-11152000 MS.gene030809:CDS 25.0%
! GTTAAATACATATAACTGGT+TGG + chr4.2:11151699-11151718 None:intergenic 25.0%
! TAAAAGTCAAAGAAATGGAT+CGG + chr4.2:11150394-11150413 None:intergenic 25.0%
! TACAAACCCTAGTAAAAAAA+AGG + chr4.2:11152254-11152273 None:intergenic 25.0%
! TATATGAATAACCTTGACTA+AGG - chr4.2:11151161-11151180 MS.gene030809:intron 25.0%
! TCAACAATTGTTAGTGAAAA+TGG + chr4.2:11151107-11151126 None:intergenic 25.0%
! TCAATAATATGATTATGGCT+GGG + chr4.2:11152152-11152171 None:intergenic 25.0%
! TCAATTTCTTCTGTAGAAAA+AGG - chr4.2:11152224-11152243 MS.gene030809:intron 25.0%
!! ATTCTTTTGACATAAATCTG+AGG + chr4.2:11151635-11151654 None:intergenic 25.0%
!! TTTTTCCTTTGTGTTTTTCA+TGG - chr4.2:11150800-11150819 MS.gene030809:intron 25.0%
!!! AGGATAACCTTTTTTTTACT+AGG - chr4.2:11152244-11152263 MS.gene030809:intron 25.0%
!!! CAAGGTATTTTCATGTAATA+AGG - chr4.2:11151192-11151211 MS.gene030809:intron 25.0%
!!! GGATAACCTTTTTTTTACTA+GGG - chr4.2:11152245-11152264 MS.gene030809:intron 25.0%
AAACAACAATATGAATGGAC+AGG + chr4.2:11150846-11150865 None:intergenic 30.0%
AATAAGCAGTGCTCAAATAA+GGG - chr4.2:11150770-11150789 MS.gene030809:intron 30.0%
ACAAAATTTCATCAGACAAG+GGG + chr4.2:11151767-11151786 None:intergenic 30.0%
AGTTCCTCAAAATATTTCTC+AGG + chr4.2:11152376-11152395 None:intergenic 30.0%
GCATAAGCATAAACATGAAA+AGG + chr4.2:11151721-11151740 None:intergenic 30.0%
GGCATTCTTCTACTAAATAA+AGG + chr4.2:11151614-11151633 None:intergenic 30.0%
GTTCCTCAAAATATTTCTCA+GGG + chr4.2:11152375-11152394 None:intergenic 30.0%
TTTAAGAAGATCTTCTCCAT+GGG + chr4.2:11150978-11150997 None:intergenic 30.0%
! GATTTACACCACGTAAATTT+TGG + chr4.2:11151572-11151591 None:intergenic 30.0%
! TAGACAAGGTATGAATGTTT+TGG - chr4.2:11152011-11152030 MS.gene030809:intron 30.0%
! TGATTCTTCTCAAAAGCTTT+TGG - chr4.2:11151790-11151809 MS.gene030809:intron 30.0%
!! AATATTTTGAGGAACTCCTT+AGG - chr4.2:11152380-11152399 MS.gene030809:intron 30.0%
!! AGAACTTTCAAGTGATTTTG+AGG - chr4.2:11151009-11151028 MS.gene030809:CDS 30.0%
!! GTCCATTCATATTGTTGTTT+CGG - chr4.2:11150846-11150865 MS.gene030809:intron 30.0%
!! GTGACATATTTTTACAGGAT+GGG - chr4.2:11150868-11150887 MS.gene030809:intron 30.0%
!! TGACATATTTTTACAGGATG+GGG - chr4.2:11150869-11150888 MS.gene030809:intron 30.0%
!! TTTCGGTGACATATTTTTAC+AGG - chr4.2:11150863-11150882 MS.gene030809:intron 30.0%
!!! CAACATTTTAGTTGCTTGAT+TGG + chr4.2:11151292-11151311 None:intergenic 30.0%
!!! TCTAGTAAGAGCATTTTCAT+CGG + chr4.2:11152428-11152447 None:intergenic 30.0%
AAAAACGTCAGATCAAGACA+TGG + chr4.2:11151743-11151762 None:intergenic 35.0%
AAAAATACACCCATCTTGGT+GGG - chr4.2:11151666-11151685 MS.gene030809:intron 35.0%
AAGTGTCTTTGTCAATTGCA+CGG + chr4.2:11152528-11152547 None:intergenic 35.0%
AAGTTTAAGGGTTTACCTTG+CGG + chr4.2:11151452-11151471 None:intergenic 35.0%
AATTGTTCACTGTCTTCTGA+TGG + chr4.2:11150343-11150362 None:intergenic 35.0%
ATAGGCAACATCTTCTTCAA+GGG + chr4.2:11151412-11151431 None:intergenic 35.0%
ATCTCCACTTGAACTTCTTA+AGG - chr4.2:11151353-11151372 MS.gene030809:intron 35.0%
ATTATTGAGGGCAGCAATTA+AGG - chr4.2:11152339-11152358 MS.gene030809:intron 35.0%
ATTGCTTCATGAGAAGATTG+CGG - chr4.2:11151883-11151902 MS.gene030809:CDS 35.0%
CAAAATTTCATCAGACAAGG+GGG + chr4.2:11151766-11151785 None:intergenic 35.0%
CACCGAAACAACAATATGAA+TGG + chr4.2:11150851-11150870 None:intergenic 35.0%
CTCAAAATATTTCTCAGGGT+AGG + chr4.2:11152371-11152390 None:intergenic 35.0%
GAATAAGCAGTGCTCAAATA+AGG - chr4.2:11150769-11150788 MS.gene030809:intron 35.0%
GATTCATCGTGATCATGATT+TGG + chr4.2:11150706-11150725 None:intergenic 35.0%
GTTTGGGAATGTAATAGACA+AGG - chr4.2:11151997-11152016 MS.gene030809:CDS 35.0%
TCATGCCATGAAAAACACAA+AGG + chr4.2:11150808-11150827 None:intergenic 35.0%
TGAAAATGCTCTTACTAGAG+TGG - chr4.2:11152429-11152448 MS.gene030809:intron 35.0%
TGATCAGGATTGTAACAACA+AGG - chr4.2:11151930-11151949 MS.gene030809:CDS 35.0%
TTATTGAGGGCAGCAATTAA+GGG - chr4.2:11152340-11152359 MS.gene030809:intron 35.0%
TTCAAACAATTCGATCATCG+TGG - chr4.2:11151314-11151333 MS.gene030809:intron 35.0%
TTCCAAAAATACACCCATCT+TGG - chr4.2:11151662-11151681 MS.gene030809:intron 35.0%
TTGACATGAAACCTTAGTCA+AGG + chr4.2:11151175-11151194 None:intergenic 35.0%
TTGACTAAGGTTTCATGTCA+AGG - chr4.2:11151174-11151193 MS.gene030809:intron 35.0%
TTTGCCTTAAGAAGTTCAAG+TGG + chr4.2:11151360-11151379 None:intergenic 35.0%
TTTGGACTACATGAAGAGAT+CGG + chr4.2:11150688-11150707 None:intergenic 35.0%
! AAAATTCTCCTTGCACTGAT+GGG - chr4.2:11152562-11152581 MS.gene030809:CDS 35.0%
! CTACCCTGAGAAATATTTTG+AGG - chr4.2:11152369-11152388 MS.gene030809:intron 35.0%
! CTTTAAGAAGATCTTCTCCA+TGG + chr4.2:11150979-11150998 None:intergenic 35.0%
! TTGTTGTTACAATCCTGATC+AGG + chr4.2:11151931-11151950 None:intergenic 35.0%
!! CTTCAAGGGACTTTTTGAAA+CGG + chr4.2:11151398-11151417 None:intergenic 35.0%
!! GGTGACATATTTTTACAGGA+TGG - chr4.2:11150867-11150886 MS.gene030809:intron 35.0%
AAGTTCAAGTGGAGATCTTG+TGG + chr4.2:11151349-11151368 None:intergenic 40.0%
ACAATCATGATGACCTGATC+AGG - chr4.2:11151915-11151934 MS.gene030809:CDS 40.0%
ACACTCCTATGTTACGTGTT+TGG - chr4.2:11150543-11150562 MS.gene030809:CDS 40.0%
ACCAACCAAACACGTAACAT+AGG + chr4.2:11150551-11150570 None:intergenic 40.0%
AGATGAGGTGAACATGACAT+TGG - chr4.2:11151844-11151863 MS.gene030809:intron 40.0%
AGTCAAAGAAATGGATCGGT+AGG + chr4.2:11150390-11150409 None:intergenic 40.0%
CAAAAATACACCCATCTTGG+TGG - chr4.2:11151665-11151684 MS.gene030809:intron 40.0%
GAAAATTCTCCTTGCACTGA+TGG - chr4.2:11152561-11152580 MS.gene030809:CDS 40.0%
GATAGGCAACATCTTCTTCA+AGG + chr4.2:11151413-11151432 None:intergenic 40.0%
GATGTTGCCTATCATACTTC+TGG - chr4.2:11151420-11151439 MS.gene030809:intron 40.0%
GCAATTGACAAAGACACTTC+TGG - chr4.2:11152529-11152548 MS.gene030809:CDS 40.0%
GCAGGATTTGGAAACTGATT+CGG - chr4.2:11152300-11152319 MS.gene030809:intron 40.0%
TCATGAGAAGATTGCGGATA+AGG - chr4.2:11151889-11151908 MS.gene030809:CDS 40.0%
TCCTATGTTACGTGTTTGGT+TGG - chr4.2:11150547-11150566 MS.gene030809:CDS 40.0%
! AGGCTGGCTATAAACAAGAT+GGG - chr4.2:11152400-11152419 MS.gene030809:intron 40.0%
! AGGCTTGATAATATCGATCC+TGG - chr4.2:11150901-11150920 MS.gene030809:CDS 40.0%
! ATTAGATCTGCCCTTTTGCT+GGG + chr4.2:11152071-11152090 None:intergenic 40.0%
! CACCAAGATGGGTGTATTTT+TGG + chr4.2:11151667-11151686 None:intergenic 40.0%
! GATGTCACCAGAAGTATGAT+AGG + chr4.2:11151430-11151449 None:intergenic 40.0%
! TAGGCTGGCTATAAACAAGA+TGG - chr4.2:11152399-11152418 MS.gene030809:intron 40.0%
! TATTAGATCTGCCCTTTTGC+TGG + chr4.2:11152072-11152091 None:intergenic 40.0%
! TCTTGTTTATAGCCAGCCTA+AGG + chr4.2:11152399-11152418 None:intergenic 40.0%
!!! AAAGCTTTTGGTTCCTCTTG+TGG - chr4.2:11151802-11151821 MS.gene030809:intron 40.0%
!!! AAGCTTTTGGTTCCTCTTGT+GGG - chr4.2:11151803-11151822 MS.gene030809:intron 40.0%
GAAGACAGTGAACAATTGCG+CGG - chr4.2:11150346-11150365 MS.gene030809:CDS 45.0%
GGCTAAAAATGCATCACGCT+CGG + chr4.2:11151271-11151290 None:intergenic 45.0%
TCACCTCATCTCCCTCATAA+CGG + chr4.2:11151835-11151854 None:intergenic 45.0%
TCACTGTCTTCTGATGGAGA+AGG + chr4.2:11150337-11150356 None:intergenic 45.0%
TCGAACTAACGTTGAGCTTC+TGG - chr4.2:11152275-11152294 MS.gene030809:intron 45.0%
TTGCAACACTCCCAGCAAAA+GGG - chr4.2:11152058-11152077 MS.gene030809:intron 45.0%
!! GGTTGATTTGCAGCGAATTG+CGG - chr4.2:11152468-11152487 MS.gene030809:intron 45.0%
!! TTTTGAGGAACTCCTTAGGC+TGG - chr4.2:11152384-11152403 MS.gene030809:intron 45.0%
!!! ATTTTTATTATTATTACTTT+TGG - chr4.2:11150461-11150480 MS.gene030809:CDS 5.0%
AAAAATGCATCACGCTCGGC+CGG + chr4.2:11151267-11151286 None:intergenic 50.0%
AATACACCCATCTTGGTGGG+TGG - chr4.2:11151669-11151688 MS.gene030809:intron 50.0%
ACACCGTTATGAGGGAGATG+AGG - chr4.2:11151829-11151848 MS.gene030809:intron 50.0%
ACAGGATGGGGAACGAATGA+AGG - chr4.2:11150881-11150900 MS.gene030809:intron 50.0%
AGTGGTGACAACTAGAGCTG+AGG - chr4.2:11152447-11152466 MS.gene030809:intron 50.0%
ATGGAGAAGGAACGTGAGAG+GGG + chr4.2:11150324-11150343 None:intergenic 50.0%
CATCATGTCCCATCAGTGCA+AGG + chr4.2:11152573-11152592 None:intergenic 50.0%
CGAATTGCGGAGGAATACCA+GGG - chr4.2:11152481-11152500 MS.gene030809:intron 50.0%
GAAACTTACACGCAGACCCA+TGG - chr4.2:11150959-11150978 MS.gene030809:CDS 50.0%
GAGCTGCATTTCTATGAGCC+AGG + chr4.2:11150922-11150941 None:intergenic 50.0%
GATGGAGAAGGAACGTGAGA+GGG + chr4.2:11150325-11150344 None:intergenic 50.0%
GGGAAGAAACAGTGTTCCTC+TGG - chr4.2:11152501-11152520 MS.gene030809:intron 50.0%
GTGGGCATACACCGTTATGA+GGG - chr4.2:11151821-11151840 MS.gene030809:intron 50.0%
GTTGCAACACTCCCAGCAAA+AGG - chr4.2:11152057-11152076 MS.gene030809:intron 50.0%
TAACGGTGTATGCCCACAAG+AGG + chr4.2:11151818-11151837 None:intergenic 50.0%
TACTTCTGGTGACATCCGCA+AGG - chr4.2:11151434-11151453 MS.gene030809:intron 50.0%
TGATGGAGAAGGAACGTGAG+AGG + chr4.2:11150326-11150345 None:intergenic 50.0%
TGTGGGCATACACCGTTATG+AGG - chr4.2:11151820-11151839 MS.gene030809:intron 50.0%
TTGTCAATTGCACGGTCCAG+AGG + chr4.2:11152520-11152539 None:intergenic 50.0%
! AACAGAAAGCTGTGCTGCTG+TGG - chr4.2:11151232-11151251 MS.gene030809:intron 50.0%
! CAATTGCGCGGTGCTTTTCA+AGG - chr4.2:11150358-11150377 MS.gene030809:CDS 50.0%
! GAGGAACACTGTTTCTTCCC+TGG + chr4.2:11152501-11152520 None:intergenic 50.0%
! TGATTTGCAGCGAATTGCGG+AGG - chr4.2:11152471-11152490 MS.gene030809:intron 50.0%
GCGAATTGCGGAGGAATACC+AGG - chr4.2:11152480-11152499 MS.gene030809:intron 55.0%
GCTGCTGTGGACATTGGATC+CGG - chr4.2:11151245-11151264 MS.gene030809:intron 55.0%
GGCTATCCACCCACCAAGAT+GGG + chr4.2:11151678-11151697 None:intergenic 55.0%
GGGTCTGCGTGTAAGTTTCG+CGG + chr4.2:11150958-11150977 None:intergenic 55.0%
TGGCTATCCACCCACCAAGA+TGG + chr4.2:11151679-11151698 None:intergenic 55.0%
! AACGTTGAGCTTCTGGCTGC+AGG - chr4.2:11152282-11152301 MS.gene030809:intron 55.0%
! AGCTGTGCTGCTGTGGACAT+TGG - chr4.2:11151239-11151258 MS.gene030809:intron 55.0%
! GAGCTTCTGGCTGCAGGATT+TGG - chr4.2:11152288-11152307 MS.gene030809:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 11150304 11152597 11150304 ID=MS.gene030809
chr4.2 mRNA 11150304 11152597 11150304 ID=MS.gene030809.t1;Parent=MS.gene030809
chr4.2 exon 11152522 11152597 11152522 ID=MS.gene030809.t1.exon1;Parent=MS.gene030809.t1
chr4.2 CDS 11152522 11152597 11152522 ID=cds.MS.gene030809.t1;Parent=MS.gene030809.t1
chr4.2 exon 11151871 11152016 11151871 ID=MS.gene030809.t1.exon2;Parent=MS.gene030809.t1
chr4.2 CDS 11151871 11152016 11151871 ID=cds.MS.gene030809.t1;Parent=MS.gene030809.t1
chr4.2 exon 11151446 11151664 11151446 ID=MS.gene030809.t1.exon3;Parent=MS.gene030809.t1
chr4.2 CDS 11151446 11151664 11151446 ID=cds.MS.gene030809.t1;Parent=MS.gene030809.t1
chr4.2 exon 11150883 11151095 11150883 ID=MS.gene030809.t1.exon4;Parent=MS.gene030809.t1
chr4.2 CDS 11150883 11151095 11150883 ID=cds.MS.gene030809.t1;Parent=MS.gene030809.t1
chr4.2 exon 11150304 11150597 11150304 ID=MS.gene030809.t1.exon5;Parent=MS.gene030809.t1
chr4.2 CDS 11150304 11150597 11150304 ID=cds.MS.gene030809.t1;Parent=MS.gene030809.t1
Gene Sequence

>MS.gene030809

ATGGCGACATTGAAGATCCCCTCTCACGTTCCTTCTCCATCAGAAGACAGTGAACAATTGCGCGGTGCTTTTCAAGGTCAACACCTACCGATCCATTTCTTTGACTTTTATTTCATCATCTATCTCTTTCATTTTTCTCGATCTATCTAGTTTGATCATTTTTATTATTATTACTTTTGGATTGCTGATAACTCTAATATCTTAATTAGATCATGGATTAATCAAATGCATAATGCATTACACTCCTATGTTACGTGTTTGGTTGGTTTATAGTATACATACTATACAATACTATTGATCGTTATATATTTCTAGTTAATGAATGTGGATGATCACTCAGATTTGATCTTGATTTTAGTTATTTATTTATTTTATTTATGTCCGATCTCTTCATGTAGTCCAAATCATGATCACGATGAATCATATAATATAATCATAACATATCGTAAAATCATAGTTGTGAATGAATAAGCAGTGCTCAAATAAGGGATCATACTTTTTCCTTTGTGTTTTTCATGGCATGAGATTTTTTTTTTATTCCTGTCCATTCATATTGTTGTTTCGGTGACATATTTTTACAGGATGGGGAACGAATGAAGGCTTGATAATATCGATCCTGGCTCATAGAAATGCAGCTCAGCGTAAGTCGATCCGCGAAACTTACACGCAGACCCATGGAGAAGATCTTCTTAAAGATCTTGACAAAGAACTTTCAAGTGATTTTGAGGTTAGTTTAAAATTTTGATATATGGCTTATTTTTTTATGTGAATCTCAATATGATGCTTATTTCATATGTATTCCATTTTCACTAACAATTGTTGACTTTTAAATTTCATAGTATTTAATTTCAGTGTTTTATATGAATAACCTTGACTAAGGTTTCATGTCAAGGTATTTTCATGTAATAAGGTTTGATGTTTTGAATATAACAGAAAGCTGTGCTGCTGTGGACATTGGATCCGGCCGAGCGTGATGCATTTTTAGCCAATCAAGCAACTAAAATGTTGACTTCAAACAATTCGATCATCGTGGAAATTGCTTCCACAAGATCTCCACTTGAACTTCTTAAGGCAAAGCAAGCATATCAAGTCCGTTTCAAAAAGTCCCTTGAAGAAGATGTTGCCTATCATACTTCTGGTGACATCCGCAAGGTAAACCCTTAAACTTACTAATTTCTTGAATCTTGATAGAGAAGAGCTGCATGTTCTAACACAATATTTAGATTCAGATGCATCTCTTTTATTTTCTTGTGAATCAAAATCTTCCAAAATTTACGTGGTGTAAATCTTTTCAACTTTAAGGTGAACCTTTATTTAGTAGAAGAATGCCTCAGATTTATGTCAAAAGAATGAGCATGTTCCAAAAATACACCCATCTTGGTGGGTGGATAGCCAACCAGTTATATGTATTTAACCTTTTCATGTTTATGCTTATGCCATGTCTTGATCTGACGTTTTTCCCCCTTGTCTGATGAAATTTTGTTTCTGATTCTTCTCAAAAGCTTTTGGTTCCTCTTGTGGGCATACACCGTTATGAGGGAGATGAGGTGAACATGACATTGGCAAAATCTGAAGCTAAATTGCTTCATGAGAAGATTGCGGATAAGGCTTACAATCATGATGACCTGATCAGGATTGTAACAACAAGGAGTAAAGCGCAATTAAATGCAACTTTGAATCACTATAACAATGAGTTTGGGAATGTAATAGACAAGGTATGAATGTTTTGGCCATTTATGATTCTTATTTCTGTGTTGCAACACTCCCAGCAAAAGGGCAGATCTAATATAATGACATAAACTTGTATTGTCTATAATGCCATTTTTCTCTTCAAGAAATATGATTCCCAGCCATAATCATATTATTGATGAAGCAATTAACTTCCTTTTAATTAAAGGGATTATAGTCTGAATTGTTAATTCAATTTCTTCTGTAGAAAAAGGATAACCTTTTTTTTACTAGGGTTTGTAGTCGAACTAACGTTGAGCTTCTGGCTGCAGGATTTGGAAACTGATTCGGATGATGAATATCTGAAATTATTGAGGGCAGCAATTAAGGGCTTGACCTACCCTGAGAAATATTTTGAGGAACTCCTTAGGCTGGCTATAAACAAGATGGGAACCGATGAAAATGCTCTTACTAGAGTGGTGACAACTAGAGCTGAGGTTGATTTGCAGCGAATTGCGGAGGAATACCAGGGAAGAAACAGTGTTCCTCTGGACCGTGCAATTGACAAAGACACTTCTGGAGATTATCAGAAAATTCTCCTTGCACTGATGGGACATGATGAGTAG
Protein sequence

>MS.gene030809.t1

MATLKIPSHVPSPSEDSEQLRGAFQGWGTNEGLIISILAHRNAAQRKSIRETYTQTHGEDLLKDLDKELSSDFEKAVLLWTLDPAERDAFLANQATKMLTSNNSIIVEIASTRSPLELLKAKQAYQVRFKKSLEEDVAYHTSGDIRKLLVPLVGIHRYEGDEVNMTLAKSEAKLLHEKIADKAYNHDDLIRIVTTRSKAQLNATLNHYNNEFGNVIDKDLETDSDDEYLKLLRAAIKGLTYPEKYFEELLRLAINKMGTDENALTRVVTTRAEVDLQRIAEEYQGRNSVPLDRAIDKDTSGDYQKILLALMGHDE