AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45772

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45772.t1 MTR_8g038150 95.016 321 9 1 1 314 1 321 0.0 626
MS.gene45772.t1 MTR_5g063670 41.270 315 175 2 1 308 1 312 2.95e-76 236
MS.gene45772.t1 MTR_8g038220 40.823 316 176 3 1 309 1 312 2.04e-75 234
MS.gene45772.t1 MTR_3g018790 41.456 316 174 3 1 309 1 312 1.53e-74 232
MS.gene45772.t1 MTR_8g038220 40.514 311 175 2 6 309 4 311 2.44e-74 231
MS.gene45772.t1 MTR_8g038180 41.640 317 174 3 1 310 1 313 2.68e-74 231
MS.gene45772.t1 MTR_0276s0050 41.456 316 175 2 1 309 1 313 3.79e-73 228
MS.gene45772.t1 MTR_3g018780 39.869 306 174 2 11 309 10 312 2.17e-72 226
MS.gene45772.t1 MTR_8g107640 40.379 317 175 4 1 308 1 312 1.28e-71 224
MS.gene45772.t1 MTR_8g038210 40.190 316 177 3 1 309 1 311 2.54e-70 221
MS.gene45772.t1 MTR_8g038210 40.830 289 167 1 21 309 27 311 1.57e-67 214
MS.gene45772.t1 MTR_6g071605 36.909 317 186 4 1 308 1 312 3.25e-62 200
MS.gene45772.t1 MTR_6g071615 38.801 317 180 4 1 308 1 312 1.84e-61 198
MS.gene45772.t1 MTR_2g031980 36.842 304 182 2 12 308 11 311 1.74e-60 196
MS.gene45772.t1 MTR_1g112520 36.593 317 187 4 1 308 1 312 6.00e-59 192
MS.gene45772.t1 MTR_1g033560 33.544 316 198 4 1 308 1 312 1.66e-57 188
MS.gene45772.t1 MTR_8g038170 37.013 308 164 8 21 310 18 313 2.91e-55 182
MS.gene45772.t1 MTR_8g038170 37.013 308 164 8 21 310 18 313 2.91e-55 182
MS.gene45772.t1 MTR_6g071595 37.457 291 175 3 20 308 27 312 3.22e-55 182
MS.gene45772.t1 MTR_3g018790 39.419 241 143 1 69 309 21 258 2.10e-51 171
MS.gene45772.t1 MTR_8g038090 90.909 77 4 2 241 314 20 96 9.94e-41 137
MS.gene45772.t1 MTR_6g071595 34.061 229 144 3 20 246 27 250 2.22e-36 132
MS.gene45772.t1 MTR_6g028030 32.143 280 179 5 38 309 34 310 1.45e-35 131
MS.gene45772.t1 MTR_3g018920 35.814 215 95 3 1 208 1 179 1.14e-33 122
MS.gene45772.t1 MTR_3g018925 40.000 90 54 0 220 309 18 107 2.38e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45772.t1 AT2G38760 55.660 318 131 3 1 308 1 318 6.77e-124 358
MS.gene45772.t1 AT5G10230 43.810 315 167 2 1 308 1 312 6.08e-82 251
MS.gene45772.t1 AT5G65020 43.396 318 169 3 1 310 1 315 4.67e-81 248
MS.gene45772.t1 AT5G12380 43.137 306 164 2 12 310 11 313 4.72e-78 241
MS.gene45772.t1 AT5G10220 41.956 317 172 3 1 308 1 314 2.12e-77 239
MS.gene45772.t1 AT5G65020 43.643 291 160 2 21 310 13 300 6.12e-75 232
MS.gene45772.t1 AT1G35720 40.312 320 174 4 1 309 1 314 1.53e-72 227
MS.gene45772.t1 AT1G68090 32.911 316 201 3 1 308 1 313 9.23e-57 186
MS.gene45772.t1 AT2G38750 32.797 311 175 8 20 309 20 317 9.49e-43 150
MS.gene45772.t1 AT2G38750 32.797 311 175 8 20 309 3 300 1.02e-42 149
MS.gene45772.t1 AT1G68090 29.644 253 167 3 1 245 1 250 1.78e-36 131

Find 58 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCCTACAAGCAACTTTA+TGG 0.111995 8.3:+58702212 MS.gene45772:CDS
CTATGCACCAACAGGTTATA+AGG 0.231265 8.3:+58703062 MS.gene45772:intron
TGATAAAGTGACAGTAAATT+TGG 0.271116 8.3:+58702070 MS.gene45772:CDS
CACTCTTAAATGTGGTAATA+TGG 0.294603 8.3:+58702439 MS.gene45772:CDS
GATCAAACAGCTGCAGATTC+TGG 0.323861 8.3:+58701611 MS.gene45772:CDS
ATGCAAAAGTTTCGCGGATC+TGG 0.343027 8.3:-58702193 None:intergenic
AAAGAGTTAATCTTGGTGTT+AGG 0.350373 8.3:+58700956 MS.gene45772:CDS
TAGACGATGATCACTTTGTT+TGG 0.352856 8.3:+58702147 MS.gene45772:CDS
GCGGATCTGGAAAACATTCC+TGG 0.367782 8.3:-58702180 None:intergenic
CTGACTGCCATCAAATGATT+TGG 0.378505 8.3:-58701657 None:intergenic
GTTATAAGGGATTCTATTGT+CGG 0.406879 8.3:+58703076 MS.gene45772:CDS
TACCTTTGCGAAATGCTTCT+CGG 0.408181 8.3:-58702475 None:intergenic
TGGGCTTAGTAAGCTCCTTC+AGG 0.412502 8.3:+58702045 MS.gene45772:CDS
GTCGAGTCCAAATCATTTGA+TGG 0.419857 8.3:+58701650 MS.gene45772:CDS
ACCTTTGCGAAATGCTTCTC+GGG 0.421680 8.3:-58702474 None:intergenic
TCTAAAATCACCAGAGAGTT+CGG 0.421715 8.3:-58701062 None:intergenic
AGACGTTCAGTGTCTGTGTT+AGG 0.424878 8.3:-58700519 None:intergenic
TATGCACCAACAGGTTATAA+GGG 0.431985 8.3:+58703063 MS.gene45772:intron
ATTTGCAAGGGATGCATTGA+AGG 0.432105 8.3:+58701575 MS.gene45772:CDS
CAAATCATTTGATGGCAGTC+AGG 0.456470 8.3:+58701658 MS.gene45772:CDS
GTGATGTATTAGTATCCCTT+TGG 0.460356 8.3:-58703274 None:intergenic
CGTCTTCAATCCGAACTCTC+TGG 0.460875 8.3:+58701052 MS.gene45772:CDS
TTTGCCATAAAGTTGCTTGT+AGG 0.463446 8.3:-58702216 None:intergenic
TCTCTTTGATTGCTCACTTG+AGG 0.469042 8.3:+58701695 MS.gene45772:CDS
TTAGGAGAAGGAACAATCTC+TGG 0.472780 8.3:-58700501 None:intergenic
TCATTTGATGGCAGTCAGGC+AGG 0.473992 8.3:+58701662 MS.gene45772:CDS
GACTTGTGATCCAGCAGAAA+GGG 0.474999 8.3:+58701545 MS.gene45772:CDS
AACAGAATGCTATAGTTCTC+TGG 0.483320 8.3:+58701523 MS.gene45772:intron
TTGCTGACTTTGCTAGGGAA+AGG 0.503452 8.3:+58703250 MS.gene45772:CDS
AGGGATTCTATTGTCGGGCT+AGG 0.506001 8.3:+58703082 MS.gene45772:CDS
CATTGAAGGTTAAGAGGAAA+GGG 0.507851 8.3:+58701589 MS.gene45772:CDS
TAAAGTGACAGTAAATTTGG+AGG 0.512807 8.3:+58702073 MS.gene45772:CDS
AGTCTTGATGATGATGTCAT+TGG 0.513392 8.3:+58703199 MS.gene45772:CDS
GGGATGCATTGAAGGTTAAG+AGG 0.521573 8.3:+58701583 MS.gene45772:CDS
CTTTGATTGCTCACTTGAGG+AGG 0.526710 8.3:+58701698 MS.gene45772:CDS
AGATACCTCGGGTGACTACA+TGG 0.528259 8.3:+58703222 MS.gene45772:CDS
TTATAAGGGATTCTATTGTC+GGG 0.533935 8.3:+58703077 MS.gene45772:CDS
GGACTTGTGATCCAGCAGAA+AGG 0.539280 8.3:+58701544 MS.gene45772:CDS
GCATTGAAGGTTAAGAGGAA+AGG 0.540378 8.3:+58701588 MS.gene45772:CDS
AAATTTGGAGGTTTCAAAGT+CGG 0.564208 8.3:+58702085 MS.gene45772:CDS
TAGAAATGCTCAACAAAGGA+AGG 0.572954 8.3:+58700982 MS.gene45772:CDS
TTGTTTGGATACTTAGCACC+AGG 0.574565 8.3:+58702162 MS.gene45772:CDS
TTGCTAGGGAAAGGACCAAA+GGG 0.576100 8.3:+58703259 MS.gene45772:CDS
AAAGGGATGCAAAATTTGCA+AGG 0.580223 8.3:+58701562 MS.gene45772:CDS
TGTAGGATGCAAAAGTTTCG+CGG 0.581239 8.3:-58702199 None:intergenic
TTATGGCAAAACATTTGAAG+AGG 0.586910 8.3:+58702229 MS.gene45772:CDS
TCAGTGTCTGTGTTAGGAGA+AGG 0.593736 8.3:-58700513 None:intergenic
GACATAGAAATGCTCAACAA+AGG 0.603561 8.3:+58700978 MS.gene45772:CDS
GATGTCATTGGAGATACCTC+GGG 0.603827 8.3:+58703211 MS.gene45772:CDS
TTTGACATCACTCTTAAATG+TGG 0.611688 8.3:+58702431 MS.gene45772:CDS
AAGGGATGCAAAATTTGCAA+GGG 0.631146 8.3:+58701563 MS.gene45772:CDS
TTTGCTAGGGAAAGGACCAA+AGG 0.633041 8.3:+58703258 MS.gene45772:CDS
CCCCGAGAAGCATTTCGCAA+AGG 0.642453 8.3:+58702473 MS.gene45772:CDS
TGTCACTTTATCATGCCTGA+AGG 0.648819 8.3:-58702060 None:intergenic
CCTTTGCGAAATGCTTCTCG+GGG 0.654248 8.3:-58702473 None:intergenic
TGATGTCATTGGAGATACCT+CGG 0.661309 8.3:+58703210 MS.gene45772:CDS
TGTGAGTGGCTGAGAAACTG+AGG 0.667057 8.3:-58701730 None:intergenic
AAAATCCATGTAGTCACCCG+AGG 0.694388 8.3:-58703227 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAAGAAAATATTGA+GGG + chr8.3:58701881-58701900 MS.gene45772:intron 10.0%
!!! CTTTTTTATGTTTTTATTAA+TGG + chr8.3:58700682-58700701 MS.gene45772:intron 10.0%
!!! TATTTTAAAAGAAAATATTG+AGG + chr8.3:58701880-58701899 MS.gene45772:intron 10.0%
!! AAAAAACTAGAATTTCAAAT+TGG - chr8.3:58701217-58701236 None:intergenic 15.0%
!! AGTTGAAATAAAAATGAAAA+CGG + chr8.3:58701936-58701955 MS.gene45772:intron 15.0%
!!! ATAACAATTTTATAAGCTTT+TGG + chr8.3:58701365-58701384 MS.gene45772:intron 15.0%
!!! ATATCTTTTTTGTATTCTTT+TGG + chr8.3:58700827-58700846 MS.gene45772:intron 15.0%
!!! ATTTTGACGAATATTTTTAT+AGG + chr8.3:58702912-58702931 MS.gene45772:intron 15.0%
!!! GTTGATTTCAATAATATATA+CGG - chr8.3:58701162-58701181 None:intergenic 15.0%
!!! TACATTATTTCTTATATCTA+AGG + chr8.3:58701388-58701407 MS.gene45772:intron 15.0%
!!! TAGTTTTTTTGTTACAAAAT+TGG + chr8.3:58701228-58701247 MS.gene45772:intron 15.0%
!!! TTTTAAAAGAAAATATTGAG+GGG + chr8.3:58701882-58701901 MS.gene45772:intron 15.0%
!! TTAAATACAGATAGAGTAAT+AGG - chr8.3:58701266-58701285 None:intergenic 20.0%
!! TTATTCATTCACTATGAATT+TGG + chr8.3:58702366-58702385 MS.gene45772:intron 20.0%
!! TTCTAACGAAAATTTCATTA+AGG + chr8.3:58702840-58702859 MS.gene45772:intron 20.0%
!! TTGAAAGAAATTACAGTAAA+TGG - chr8.3:58702981-58703000 None:intergenic 20.0%
!!! CTTTTAAAATACTTGAAAAC+AGG - chr8.3:58701871-58701890 None:intergenic 20.0%
!!! GAGGAATTTTTTTTATTCAT+TGG + chr8.3:58700768-58700787 MS.gene45772:intron 20.0%
! AAATGTTGTTAGATAGATTG+TGG + chr8.3:58701827-58701846 MS.gene45772:intron 25.0%
! ACATCATTATCTTGACTTAT+AGG - chr8.3:58701340-58701359 None:intergenic 25.0%
! AGAAAACACATAATATGTGT+CGG - chr8.3:58702592-58702611 None:intergenic 25.0%
! AGATGAAAAAGAGTTAATCT+TGG + chr8.3:58700949-58700968 MS.gene45772:CDS 25.0%
! CATCATTACTTCAGAAAAAA+TGG - chr8.3:58701460-58701479 None:intergenic 25.0%
! GAACATCATTATACTAATTG+AGG - chr8.3:58702335-58702354 None:intergenic 25.0%
! GGTTATTGGAAAATTTATGT+AGG + chr8.3:58700917-58700936 MS.gene45772:intron 25.0%
! TAACGAAAATTTCATTAAGG+CGG + chr8.3:58702843-58702862 MS.gene45772:intron 25.0%
! TACATCATCTCAAAAAGAAA+AGG - chr8.3:58702766-58702785 None:intergenic 25.0%
! TCTCAAAAATGAAAAGTCAA+TGG - chr8.3:58701113-58701132 None:intergenic 25.0%
! TGAATGAATAAACAACATCT+TGG - chr8.3:58702357-58702376 None:intergenic 25.0%
! TGATAAAGTGACAGTAAATT+TGG + chr8.3:58702070-58702089 MS.gene45772:CDS 25.0%
! TGGAAAATTTATGTAGGAAT+TGG + chr8.3:58700923-58700942 MS.gene45772:intron 25.0%
! TTGAATTTGTGAAGCAAATT+AGG - chr8.3:58701984-58702003 None:intergenic 25.0%
!! TGTTTCTTTTAGATTTTGAG+AGG - chr8.3:58702669-58702688 None:intergenic 25.0%
AAATTTGGAGGTTTCAAAGT+CGG + chr8.3:58702085-58702104 MS.gene45772:CDS 30.0%
CACTCTTAAATGTGGTAATA+TGG + chr8.3:58702439-58702458 MS.gene45772:CDS 30.0%
CATCTCAAAAAGAAAAGGAA+GGG - chr8.3:58702761-58702780 None:intergenic 30.0%
GACATAAAAACTTGTGGTAA+AGG + chr8.3:58702405-58702424 MS.gene45772:CDS 30.0%
GACTTATAGGTGTAAAATTG+AGG - chr8.3:58701327-58701346 None:intergenic 30.0%
GTTATAAGGGATTCTATTGT+CGG + chr8.3:58703076-58703095 MS.gene45772:CDS 30.0%
GTTCTTCAAACATATCATGT+GGG + chr8.3:58702005-58702024 MS.gene45772:intron 30.0%
TAAAGTGACAGTAAATTTGG+AGG + chr8.3:58702073-58702092 MS.gene45772:CDS 30.0%
TCATCTCAAAAAGAAAAGGA+AGG - chr8.3:58702762-58702781 None:intergenic 30.0%
TGAATCACAAAGATTTGTAG+AGG + chr8.3:58700749-58700768 MS.gene45772:intron 30.0%
TGTTCTTCAAACATATCATG+TGG + chr8.3:58702004-58702023 MS.gene45772:intron 30.0%
TTACAGGACATAAAAACTTG+TGG + chr8.3:58702399-58702418 MS.gene45772:intron 30.0%
TTATAAGGGATTCTATTGTC+GGG + chr8.3:58703077-58703096 MS.gene45772:CDS 30.0%
TTATCTAAGTTTGTCTAGCT+AGG + chr8.3:58701797-58701816 MS.gene45772:intron 30.0%
TTATGGCAAAACATTTGAAG+AGG + chr8.3:58702229-58702248 MS.gene45772:CDS 30.0%
TTTGACATCACTCTTAAATG+TGG + chr8.3:58702431-58702450 MS.gene45772:CDS 30.0%
!! AAAGAGTTAATCTTGGTGTT+AGG + chr8.3:58700956-58700975 MS.gene45772:CDS 30.0%
!! ATTTGGCTGATTTTCTTTAC+AGG + chr8.3:58702383-58702402 MS.gene45772:intron 30.0%
!! ATTTTTGTATCGATGGTTGT+TGG + chr8.3:58700863-58700882 MS.gene45772:intron 30.0%
!! CATTGAGAATTTAGCTTGTT+TGG + chr8.3:58702527-58702546 MS.gene45772:intron 30.0%
!! TCTACAATGAACTTTTTCCT+TGG + chr8.3:58702689-58702708 MS.gene45772:intron 30.0%
!! TTGATGTTGATGATGGTTAT+TGG + chr8.3:58700903-58700922 MS.gene45772:intron 30.0%
!!! ATTTTTTGCTGACTTTGCTA+GGG + chr8.3:58703245-58703264 MS.gene45772:CDS 30.0%
!!! TATGCTGATTTTTGTATCGA+TGG + chr8.3:58700856-58700875 MS.gene45772:intron 30.0%
!!! TTTTTATAGGAGTACCAACA+TGG + chr8.3:58702925-58702944 MS.gene45772:intron 30.0%
AAAGAGAAAGAGAGAAACCT+TGG - chr8.3:58700557-58700576 None:intergenic 35.0%
AAAGGGATGCAAAATTTGCA+AGG + chr8.3:58701562-58701581 MS.gene45772:CDS 35.0%
AACAGAATGCTATAGTTCTC+TGG + chr8.3:58701523-58701542 MS.gene45772:intron 35.0%
AAGATGAAATGCAACCATGT+TGG - chr8.3:58702942-58702961 None:intergenic 35.0%
AAGGGATGCAAAATTTGCAA+GGG + chr8.3:58701563-58701582 MS.gene45772:CDS 35.0%
CATTGAAGGTTAAGAGGAAA+GGG + chr8.3:58701589-58701608 MS.gene45772:CDS 35.0%
GACATAGAAATGCTCAACAA+AGG + chr8.3:58700978-58700997 MS.gene45772:CDS 35.0%
TAGAAATGCTCAACAAAGGA+AGG + chr8.3:58700982-58701001 MS.gene45772:CDS 35.0%
TAGAATCCCTTATAACCTGT+TGG - chr8.3:58703072-58703091 None:intergenic 35.0%
TAGACGATGATCACTTTGTT+TGG + chr8.3:58702147-58702166 MS.gene45772:CDS 35.0%
TATAATCGACCATCTTTCCA+AGG - chr8.3:58702709-58702728 None:intergenic 35.0%
TATGCACCAACAGGTTATAA+GGG + chr8.3:58703063-58703082 MS.gene45772:intron 35.0%
TTTGCCATAAAGTTGCTTGT+AGG - chr8.3:58702219-58702238 None:intergenic 35.0%
! GAGATTGTTGATGTTGATGA+TGG + chr8.3:58700896-58700915 MS.gene45772:intron 35.0%
! TCTAAAATCACCAGAGAGTT+CGG - chr8.3:58701065-58701084 None:intergenic 35.0%
!! AGTCTTGATGATGATGTCAT+TGG + chr8.3:58703199-58703218 MS.gene45772:CDS 35.0%
!! GAACTTTTTCCTTGGAAAGA+TGG + chr8.3:58702697-58702716 MS.gene45772:intron 35.0%
!! GGTGTTATGCAGATTTTAGT+GGG + chr8.3:58702026-58702045 MS.gene45772:intron 35.0%
!!! GATTTTTTGCTGACTTTGCT+AGG + chr8.3:58703244-58703263 MS.gene45772:CDS 35.0%
ATTTGCAAGGGATGCATTGA+AGG + chr8.3:58701575-58701594 MS.gene45772:CDS 40.0%
CGTCTTAGAAATGCTTTCCA+AGG + chr8.3:58700537-58700556 MS.gene45772:intron 40.0%
CTATGCACCAACAGGTTATA+AGG + chr8.3:58703062-58703081 MS.gene45772:intron 40.0%
CTGACTGCCATCAAATGATT+TGG - chr8.3:58701660-58701679 None:intergenic 40.0%
GCATCCTACAAGCAACTTTA+TGG + chr8.3:58702212-58702231 MS.gene45772:CDS 40.0%
GCATTGAAGGTTAAGAGGAA+AGG + chr8.3:58701588-58701607 MS.gene45772:CDS 40.0%
GTCGAGTCCAAATCATTTGA+TGG + chr8.3:58701650-58701669 MS.gene45772:CDS 40.0%
TACCTTTGCGAAATGCTTCT+CGG - chr8.3:58702478-58702497 None:intergenic 40.0%
TCTCTTTGATTGCTCACTTG+AGG + chr8.3:58701695-58701714 MS.gene45772:CDS 40.0%
TGATGTCATTGGAGATACCT+CGG + chr8.3:58703210-58703229 MS.gene45772:CDS 40.0%
TGTAGGATGCAAAAGTTTCG+CGG - chr8.3:58702202-58702221 None:intergenic 40.0%
TTAGGAGAAGGAACAATCTC+TGG - chr8.3:58700504-58700523 None:intergenic 40.0%
TTGTTTGGATACTTAGCACC+AGG + chr8.3:58702162-58702181 MS.gene45772:CDS 40.0%
! AATTTTGCATCCCTTTCTGC+TGG - chr8.3:58701558-58701577 None:intergenic 40.0%
! CAAATCATTTGATGGCAGTC+AGG + chr8.3:58701658-58701677 MS.gene45772:CDS 40.0%
!! CATGTCCTTACTTTTGTGAG+TGG - chr8.3:58701747-58701766 None:intergenic 40.0%
!! GGGTGTTATGCAGATTTTAG+TGG + chr8.3:58702025-58702044 MS.gene45772:intron 40.0%
!! TGTCACTTTATCATGCCTGA+AGG - chr8.3:58702063-58702082 None:intergenic 40.0%
AAAATCCATGTAGTCACCCG+AGG - chr8.3:58703230-58703249 None:intergenic 45.0%
ACCTTTGCGAAATGCTTCTC+GGG - chr8.3:58702477-58702496 None:intergenic 45.0%
AGACGTTCAGTGTCTGTGTT+AGG - chr8.3:58700522-58700541 None:intergenic 45.0%
ATGCAAAAGTTTCGCGGATC+TGG - chr8.3:58702196-58702215 None:intergenic 45.0%
CTCAGCCACTCACAAAAGTA+AGG + chr8.3:58701739-58701758 MS.gene45772:intron 45.0%
CTTTGATTGCTCACTTGAGG+AGG + chr8.3:58701698-58701717 MS.gene45772:CDS 45.0%
GACTTGTGATCCAGCAGAAA+GGG + chr8.3:58701545-58701564 MS.gene45772:CDS 45.0%
GATCAAACAGCTGCAGATTC+TGG + chr8.3:58701611-58701630 MS.gene45772:CDS 45.0%
GATGTCATTGGAGATACCTC+GGG + chr8.3:58703211-58703230 MS.gene45772:CDS 45.0%
GGGATGCATTGAAGGTTAAG+AGG + chr8.3:58701583-58701602 MS.gene45772:CDS 45.0%
TCACACTTCTATGCACCAAC+AGG + chr8.3:58703054-58703073 MS.gene45772:intron 45.0%
TCAGTGTCTGTGTTAGGAGA+AGG - chr8.3:58700516-58700535 None:intergenic 45.0%
TTGCTAGGGAAAGGACCAAA+GGG + chr8.3:58703259-58703278 MS.gene45772:CDS 45.0%
TTTGCTAGGGAAAGGACCAA+AGG + chr8.3:58703258-58703277 MS.gene45772:CDS 45.0%
!! TTGCTGACTTTGCTAGGGAA+AGG + chr8.3:58703250-58703269 MS.gene45772:CDS 45.0%
!!! TTTTTTATGTTTTTATTAAT+GGG + chr8.3:58700683-58700702 MS.gene45772:intron 5.0%
AGATACCTCGGGTGACTACA+TGG + chr8.3:58703222-58703241 MS.gene45772:CDS 50.0%
AGGGATTCTATTGTCGGGCT+AGG + chr8.3:58703082-58703101 MS.gene45772:CDS 50.0%
CCTTTGCGAAATGCTTCTCG+GGG - chr8.3:58702476-58702495 None:intergenic 50.0%
CGTCTTCAATCCGAACTCTC+TGG + chr8.3:58701052-58701071 MS.gene45772:CDS 50.0%
GCGGATCTGGAAAACATTCC+TGG - chr8.3:58702183-58702202 None:intergenic 50.0%
GGACTTGTGATCCAGCAGAA+AGG + chr8.3:58701544-58701563 MS.gene45772:CDS 50.0%
TGGGCTTAGTAAGCTCCTTC+AGG + chr8.3:58702045-58702064 MS.gene45772:CDS 50.0%
TGTGAGTGGCTGAGAAACTG+AGG - chr8.3:58701733-58701752 None:intergenic 50.0%
! TCATTTGATGGCAGTCAGGC+AGG + chr8.3:58701662-58701681 MS.gene45772:CDS 50.0%
!! CCCCGAGAAGCATTTCGCAA+AGG + chr8.3:58702473-58702492 MS.gene45772:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 58700483 58703288 58700483 ID=MS.gene45772
chr8.3 mRNA 58700483 58703288 58700483 ID=MS.gene45772.t1;Parent=MS.gene45772
chr8.3 exon 58700483 58700537 58700483 ID=MS.gene45772.t1.exon1;Parent=MS.gene45772.t1
chr8.3 CDS 58700483 58700537 58700483 ID=cds.MS.gene45772.t1;Parent=MS.gene45772.t1
chr8.3 exon 58700939 58701084 58700939 ID=MS.gene45772.t1.exon2;Parent=MS.gene45772.t1
chr8.3 CDS 58700939 58701084 58700939 ID=cds.MS.gene45772.t1;Parent=MS.gene45772.t1
chr8.3 exon 58701528 58701755 58701528 ID=MS.gene45772.t1.exon3;Parent=MS.gene45772.t1
chr8.3 CDS 58701528 58701755 58701528 ID=cds.MS.gene45772.t1;Parent=MS.gene45772.t1
chr8.3 exon 58702038 58702250 58702038 ID=MS.gene45772.t1.exon4;Parent=MS.gene45772.t1
chr8.3 CDS 58702038 58702250 58702038 ID=cds.MS.gene45772.t1;Parent=MS.gene45772.t1
chr8.3 exon 58702405 58702494 58702405 ID=MS.gene45772.t1.exon5;Parent=MS.gene45772.t1
chr8.3 CDS 58702405 58702494 58702405 ID=cds.MS.gene45772.t1;Parent=MS.gene45772.t1
chr8.3 exon 58703076 58703288 58703076 ID=MS.gene45772.t1.exon6;Parent=MS.gene45772.t1
chr8.3 CDS 58703076 58703288 58703076 ID=cds.MS.gene45772.t1;Parent=MS.gene45772.t1
Gene Sequence

>MS.gene45772

ATGGCTTCATTGAAACTACCAGAGATTGTTCCTTCTCCTAACACAGACACTGAACGTCTTAGAAATGCTTTCCAAGGTTTCTCTCTTTCTCTTTCTCTCTCTAATGCTATGAAAGTTTTTTAAAAAAAAAACTTTTTTTTTTAATGATGTTAAGTATTTTTCTGAATTTGTGTAGCGAGAGTTATGTTAAAAAACTGAACTTTTTTATGTTTTTATTAATGGGTTTTTTCAGAAACGTTACTTTATAATATGCATGTTTGTAAAATTGAATCACAAAGATTTGTAGAGGAATTTTTTTTATTCATTGGACAACATTGAAAGTACTGTTATGATTTTGTCATTTTATATCTTTTTTGTATTCTTTTGGTTCATTTATGCTGATTTTTGTATCGATGGTTGTTGGTATTAGAAATGAGATTGTTGATGTTGATGATGGTTATTGGAAAATTTATGTAGGAATTGGAACAGATGAAAAAGAGTTAATCTTGGTGTTAGGACATAGAAATGCTCAACAAAGGAAGGAAATCAGAGAAACTTATCAGCAACGTTACAATGAATCGCTTCTTGATCGTCTTCAATCCGAACTCTCTGGTGATTTTAGAGTATGTCATCATGTCATTCTTGCTTCCATTGACTTTTCATTTTTGAGAAACAATTCTTCAATGTCGTATTTTATCCGTATATATTATTGAAATCAACTGAAATTATGTCATTTTCAGTGATTGCTTGTGCCAATTTGAAATTCTAGTTTTTTTGTTACAAAATTGGTCAATCATGTGTCCTATTACTCTATCTGTATTTAAATTTTGAGCTAATTTGATTTGAGCATTACAAATATAAACCTCAATTTTACACCTATAAGTCAAGATAATGATGTTTGTCATAACAATTTTATAAGCTTTTGGTACATTATTTCTTATATCTAAGGAAAAGTTCATTTTATCGTCGTATTTCAATATGATTTTCGAAGCGTGCCATTTTTTCTGAAGTAATGATGCGATCTACAATGCAATATCATTACATAGAATAGTGTGATATGTAACAGAATGCTATAGTTCTCTGGACTTGTGATCCAGCAGAAAGGGATGCAAAATTTGCAAGGGATGCATTGAAGGTTAAGAGGAAAGGGATCAAACAGCTGCAGATTCTGGTTGAAATAGCATGCGCGTCGAGTCCAAATCATTTGATGGCAGTCAGGCAGGCTTACTGCTCTCTCTTTGATTGCTCACTTGAGGAGGACATTATAGCCTCAGTTTCTCAGCCACTCACAAAAGTAAGGACATGAAGTTTCTTATTCGCATATAAAAGAAATTCTTATCTAAGTTTGTCTAGCTAGGTTCAAGTAAATGTTGTTAGATAGATTGTGGCTAAAAGAAAATGTTAGACCTGTTTTCAAGTATTTTAAAAGAAAATATTGAGGGGAATGTTGAGTCTATATTCTCATATGTTTATTAGTTGAAATAAAAATGAAAACGGAAAACATGAAAAGAAAACGAGTCCTAATTTGCTTCACAAATTCAATGTTCTTCAAACATATCATGTGGGTGTTATGCAGATTTTAGTGGGCTTAGTAAGCTCCTTCAGGCATGATAAAGTGACAGTAAATTTGGAGGTTTCAAAGTCGGAAGCAGAAAAACTACATGAAGCAATCAACAATAAGAAATTAGACGATGATCACTTTGTTTGGATACTTAGCACCAGGAATGTTTTCCAGATCCGCGAAACTTTTGCATCCTACAAGCAACTTTATGGCAAAACATTTGAAGAGGTTTTGTTTAACGATTTCAATTTTAATGTCAACTAGTAATTTAAATCATGTCACTCGTTCAAAATGTAGTTAACAGAAAAGCCTCAATTAGTATAATGATGTTCCAAGATGTTGTTTATTCATTCACTATGAATTTGGCTGATTTTCTTTACAGGACATAAAAACTTGTGGTAAAGGTGATTTGACATCACTCTTAAATGTGGTAATATGGTGTATAGAATGCCCCGAGAAGCATTTCGCAAAGGTACTTGTTAATTCTCTTCTACTGATAGCTCGCATTGAGAATTTAGCTTGTTTGGTTGAGATTCTTCTCTATGCAAAGACGATAATTGCATACTCCGACACATATTATGTGTTTTCTGACACTTTTAATCAAAGCAAATTTGTCTTGCTTCTAATCGAATTGTGTTTGAAACCTCTCAAAATCTAAAAGAAACATCTACAATGAACTTTTTCCTTGGAAAGATGGTCGATTATACATTACAGTAATGTTTCATTCAATTTTTACCCTTCCTTTTCTTTTTGAGATGATGTATATGTACTAATTCATTTTTTTCGTTCAAATATACAATGTATCTTTCGTTCAATCTTCTAACGAAAATTTCATTAAGGCGGTTTCAAAGATCAAATTTTGACATCAATGCAGTCGTTTTATATGACTATTTTGACGAATATTTTTATAGGAGTACCAACATGGTTGCATTTCATCTTTAAAATATCAAAGTATCCATTTACTGTAATTTCTTTCAAGCTATATCAAGATTACTCATTCAGAAAATGATTGCAATTAATCTCATTTCTGTTCACACTTCTATGCACCAACAGGTTATAAGGGATTCTATTGTCGGGCTAGGAACTGATGAAGATTCACTCAACAGAGCTATTGTAACTCGAGCTGAAATCGACTTGTTGAAAGTGAGATTCGAGTATGCTAACATGTACAAATCAAGTCTTGATGATGATGTCATTGGAGATACCTCGGGTGACTACATGGATTTTTTGCTGACTTTGCTAGGGAAAGGACCAAAGGGATACTAA
Protein sequence

>MS.gene45772.t1

MASLKLPEIVPSPNTDTERIGTDEKELILVLGHRNAQQRKEIRETYQQRYNESLLDRLQSELSGDFRNAIVLWTCDPAERDAKFARDALKVKRKGIKQLQILVEIACASSPNHLMAVRQAYCSLFDCSLEEDIIASVSQPLTKILVGLVSSFRHDKVTVNLEVSKSEAEKLHEAINNKKLDDDHFVWILSTRNVFQIRETFASYKQLYGKTFEEDIKTCGKGDLTSLLNVVIWCIECPEKHFAKVIRDSIVGLGTDEDSLNRAIVTRAEIDLLKVRFEYANMYKSSLDDDVIGDTSGDYMDFLLTLLGKGPKGY