AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92405

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92405.t1 MTR_8g038150 93.750 320 13 1 1 313 1 320 0.0 613
MS.gene92405.t1 MTR_5g063670 42.222 315 172 2 1 308 1 312 1.17e-77 239
MS.gene92405.t1 MTR_0276s0050 41.456 316 175 2 1 309 1 313 1.04e-73 229
MS.gene92405.t1 MTR_8g038220 39.873 316 179 3 1 309 1 312 2.36e-73 229
MS.gene92405.t1 MTR_8g038220 39.550 311 178 2 6 309 4 311 2.48e-72 226
MS.gene92405.t1 MTR_8g038180 40.379 317 178 3 1 310 1 313 5.27e-72 225
MS.gene92405.t1 MTR_8g107640 41.009 317 173 4 1 308 1 312 1.39e-71 224
MS.gene92405.t1 MTR_3g018790 40.190 316 178 3 1 309 1 312 1.61e-71 224
MS.gene92405.t1 MTR_3g018780 38.889 306 177 2 11 309 10 312 7.06e-70 220
MS.gene92405.t1 MTR_8g038210 39.241 316 180 3 1 309 1 311 5.39e-68 215
MS.gene92405.t1 MTR_8g038210 39.792 289 170 1 21 309 27 311 2.00e-65 208
MS.gene92405.t1 MTR_6g071605 36.909 317 186 4 1 308 1 312 1.84e-61 198
MS.gene92405.t1 MTR_2g031980 37.500 304 180 2 12 308 11 311 5.63e-61 197
MS.gene92405.t1 MTR_6g071615 38.801 317 180 4 1 308 1 312 1.75e-60 196
MS.gene92405.t1 MTR_1g112520 36.593 317 187 4 1 308 1 312 4.93e-58 189
MS.gene92405.t1 MTR_1g033560 33.861 316 197 4 1 308 1 312 1.01e-57 188
MS.gene92405.t1 MTR_8g038170 38.312 308 160 8 21 310 18 313 6.83e-56 184
MS.gene92405.t1 MTR_8g038170 38.312 308 160 8 21 310 18 313 6.83e-56 184
MS.gene92405.t1 MTR_6g071595 37.801 291 174 3 20 308 27 312 8.63e-55 181
MS.gene92405.t1 MTR_3g018790 37.759 241 147 1 69 309 21 258 1.67e-48 163
MS.gene92405.t1 MTR_8g038090 90.789 76 4 2 241 313 20 95 8.15e-40 135
MS.gene92405.t1 MTR_6g071595 34.498 229 143 3 20 246 27 250 3.57e-36 131
MS.gene92405.t1 MTR_6g028030 32.143 280 179 5 38 309 34 310 6.93e-35 129
MS.gene92405.t1 MTR_3g018920 34.884 215 97 3 1 208 1 179 7.84e-32 117
MS.gene92405.t1 MTR_3g018925 41.463 82 48 0 228 309 26 107 5.04e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92405.t1 AT2G38760 54.717 318 134 3 1 308 1 318 5.90e-122 353
MS.gene92405.t1 AT5G10230 43.492 315 168 2 1 308 1 312 1.15e-81 250
MS.gene92405.t1 AT5G65020 43.082 318 170 3 1 310 1 315 1.44e-80 247
MS.gene92405.t1 AT5G12380 42.810 306 165 2 12 310 11 313 1.87e-77 239
MS.gene92405.t1 AT5G10220 41.640 317 173 3 1 308 1 314 1.95e-77 239
MS.gene92405.t1 AT5G65020 43.299 291 161 2 21 310 13 300 1.50e-74 231
MS.gene92405.t1 AT1G35720 40.000 320 175 4 1 309 1 314 1.04e-71 224
MS.gene92405.t1 AT1G68090 33.228 316 200 3 1 308 1 313 1.36e-56 186
MS.gene92405.t1 AT2G38750 33.762 311 172 8 20 309 3 300 1.39e-42 149
MS.gene92405.t1 AT2G38750 33.762 311 172 8 20 309 20 317 1.51e-42 149
MS.gene92405.t1 AT1G68090 30.040 253 166 3 1 245 1 250 2.25e-36 131

Find 60 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGCACCAACAGGTTATA+AGG 0.231265 8.4:+60246066 MS.gene92405:intron
GTTATAAGGGATTCTATTGT+TGG 0.321656 8.4:+60246080 MS.gene92405:CDS
GATCAAACAGCTGCAGATTC+TGG 0.323861 8.4:+60244541 MS.gene92405:CDS
CGCTCTTAAATGTGGTAGTA+TGG 0.329332 8.4:+60245369 MS.gene92405:CDS
TAGATGATGATCACTTTGTT+TGG 0.349916 8.4:+60245077 MS.gene92405:CDS
AAAGAGTTAATCTTGGTGTT+AGG 0.350373 8.4:+60243886 MS.gene92405:CDS
ATGCAAGAGTTTCACGGATC+TGG 0.369500 8.4:-60245123 None:intergenic
GCATCCTACAAGCAACTCTA+TGG 0.375541 8.4:+60245142 MS.gene92405:CDS
CTGACTGCCATCAAATGATT+TGG 0.378505 8.4:-60244587 None:intergenic
ACGGATCTGGAAAACATTCC+TGG 0.388969 8.4:-60245110 None:intergenic
GTCGAGTCCAAATCATTTGA+TGG 0.419857 8.4:+60244580 MS.gene92405:CDS
TCTAAAATCACCAGAGAGTT+CGG 0.421715 8.4:-60243992 None:intergenic
TACCTTTGCGAAATGTTTCT+CGG 0.422016 8.4:-60245405 None:intergenic
AGACGTTCAGTGTCTGTGTT+AGG 0.424878 8.4:-60243449 None:intergenic
TATGCACCAACAGGTTATAA+GGG 0.431985 8.4:+60246067 MS.gene92405:intron
ATTTGCAAGGGATGCATTGA+AGG 0.432105 8.4:+60244505 MS.gene92405:CDS
ACCTTTGCGAAATGTTTCTC+GGG 0.432951 8.4:-60245404 None:intergenic
CAAATCATTTGATGGCAGTC+AGG 0.456470 8.4:+60244588 MS.gene92405:CDS
CGTCTTCAATCCGAACTCTC+TGG 0.460875 8.4:+60243982 MS.gene92405:CDS
TCTCTTTGATTGCTCACTTG+AGG 0.469042 8.4:+60244625 MS.gene92405:CDS
TTGCTGACTTTGCTAGGGAA+GGG 0.469762 8.4:+60246254 MS.gene92405:CDS
TTAGGAGAAGGAACAATCTC+TGG 0.472780 8.4:-60243431 None:intergenic
TCATTTGATGGCAGTCAGGC+AGG 0.473992 8.4:+60244592 MS.gene92405:CDS
TTATAAGGGATTCTATTGTT+GGG 0.473994 8.4:+60246081 MS.gene92405:CDS
GACTTGTGATCCAGCAGAAA+GGG 0.474999 8.4:+60244475 MS.gene92405:CDS
AGGGATTCTATTGTTGGGCT+AGG 0.475438 8.4:+60246086 MS.gene92405:CDS
TTTGCCATAGAGTTGCTTGT+AGG 0.478328 8.4:-60245146 None:intergenic
TAATAAAGTGACAGTAAGCT+TGG 0.481162 8.4:+60245000 MS.gene92405:CDS
AACAGAATGCTATAGTTCTC+TGG 0.483320 8.4:+60244453 MS.gene92405:intron
GTGATGTATTAGCATCCCTT+TGG 0.507732 8.4:-60246278 None:intergenic
CATTGAAGGTTAAGAGGAAA+GGG 0.507851 8.4:+60244519 MS.gene92405:CDS
AGTCTTGATGATGATGTCAT+TGG 0.513392 8.4:+60246203 MS.gene92405:CDS
TTTGCTGACTTTGCTAGGGA+AGG 0.520124 8.4:+60246253 MS.gene92405:CDS
GGGATGCATTGAAGGTTAAG+AGG 0.521573 8.4:+60244513 MS.gene92405:CDS
CTATGGCAAAACATTGGAAG+AGG 0.523562 8.4:+60245159 MS.gene92405:CDS
CTTTGATTGCTCACTTGAGG+AGG 0.526710 8.4:+60244628 MS.gene92405:CDS
AGATACCTCGGGTGACTACA+TGG 0.528259 8.4:+60246226 MS.gene92405:CDS
TGTAGGATGCAAGAGTTTCA+CGG 0.538698 8.4:-60245129 None:intergenic
GGACTTGTGATCCAGCAGAA+AGG 0.539280 8.4:+60244474 MS.gene92405:CDS
GCATTGAAGGTTAAGAGGAA+AGG 0.540378 8.4:+60244518 MS.gene92405:CDS
TGGGCTTAGTAAGCTCCTAC+AGG 0.549295 8.4:+60244975 MS.gene92405:CDS
AAGCTTGGAGGTTGCAAAGT+CGG 0.554293 8.4:+60245015 MS.gene92405:CDS
TTTAATATCGCTCTTAAATG+TGG 0.560661 8.4:+60245361 MS.gene92405:CDS
TTGCTAGGGAAGGGACCAAA+GGG 0.566608 8.4:+60246263 MS.gene92405:CDS
TAGAAATGCTCAACAAAGGA+AGG 0.572954 8.4:+60243912 MS.gene92405:CDS
TTGTTTGGATACTTAGCACC+AGG 0.574565 8.4:+60245092 MS.gene92405:CDS
AAAGGGATGCAAAATTTGCA+AGG 0.580223 8.4:+60244492 MS.gene92405:CDS
TGTCACTTTATTATGCCTGT+AGG 0.584848 8.4:-60244990 None:intergenic
TAAAGTGACAGTAAGCTTGG+AGG 0.586007 8.4:+60245003 MS.gene92405:CDS
TTTGCTAGGGAAGGGACCAA+AGG 0.591319 8.4:+60246262 MS.gene92405:CDS
GCAACTCTATGGCAAAACAT+TGG 0.593185 8.4:+60245153 MS.gene92405:CDS
TCAGTGTCTGTGTTAGGAGA+AGG 0.593736 8.4:-60243443 None:intergenic
GACATAGAAATGCTCAACAA+AGG 0.603561 8.4:+60243908 MS.gene92405:CDS
GATGTCATTGGAGATACCTC+GGG 0.603827 8.4:+60246215 MS.gene92405:CDS
AAGGGATGCAAAATTTGCAA+GGG 0.631146 8.4:+60244493 MS.gene92405:CDS
CCCCGAGAAACATTTCGCAA+AGG 0.634664 8.4:+60245403 MS.gene92405:CDS
CCTTTGCGAAATGTTTCTCG+GGG 0.655192 8.4:-60245403 None:intergenic
TGATGTCATTGGAGATACCT+CGG 0.661309 8.4:+60246214 MS.gene92405:CDS
TGTGAGTGGCTGAGAAACTG+AGG 0.667057 8.4:-60244660 None:intergenic
AAAATCCATGTAGTCACCCG+AGG 0.694388 8.4:-60246231 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAAGAAAATATTGA+GGG + chr8.4:60244811-60244830 MS.gene92405:intron 10.0%
!!! CTTTTTTATGTTTTTATTAA+TGG + chr8.4:60243612-60243631 MS.gene92405:intron 10.0%
!!! TATTTTAAAAGAAAATATTG+AGG + chr8.4:60244810-60244829 MS.gene92405:intron 10.0%
!! AAAAAACTAGAATTTCAAAT+TGG - chr8.4:60244147-60244166 None:intergenic 15.0%
!! ATTTACAATATTGATAAACT+TGG - chr8.4:60245655-60245674 None:intergenic 15.0%
!! TTCTAATGAAAATTTCATTA+AGG + chr8.4:60245854-60245873 MS.gene92405:intron 15.0%
!!! ATAACAATTTTATAAGCTTT+TGG + chr8.4:60244295-60244314 MS.gene92405:intron 15.0%
!!! ATATCTTTTTTGTATTCTTT+TGG + chr8.4:60243757-60243776 MS.gene92405:intron 15.0%
!!! GTTGATTTCAATAATATATA+CGG - chr8.4:60244092-60244111 None:intergenic 15.0%
!!! TACATTATTTCTTATATCTA+AGG + chr8.4:60244318-60244337 MS.gene92405:intron 15.0%
!!! TAGTTTTTTTGTTACAAAAT+TGG + chr8.4:60244158-60244177 MS.gene92405:intron 15.0%
!!! TTTTAAAAGAAAATATTGAG+GGG + chr8.4:60244812-60244831 MS.gene92405:intron 15.0%
!! AAAATGAAATGCAAAAATGT+TGG - chr8.4:60245952-60245971 None:intergenic 20.0%
!! AGTTGAACTATAAATGAAAA+CGG + chr8.4:60244866-60244885 MS.gene92405:intron 20.0%
!! TAATGAAAATTTCATTAAGG+TGG + chr8.4:60245857-60245876 MS.gene92405:intron 20.0%
!! TATTCATTCACTATGAATTT+GGG + chr8.4:60245297-60245316 MS.gene92405:intron 20.0%
!! TTAAATACAGATAGAGTAAT+AGG - chr8.4:60244196-60244215 None:intergenic 20.0%
!! TTATTCATTCACTATGAATT+TGG + chr8.4:60245296-60245315 MS.gene92405:intron 20.0%
!! TTGAAAGAAATTACAGTAAA+TGG - chr8.4:60245991-60246010 None:intergenic 20.0%
!!! CTTTTAAAATACTTGAAAAC+AGG - chr8.4:60244801-60244820 None:intergenic 20.0%
!!! GAGGAATTTTTTTTATTCAT+TGG + chr8.4:60243698-60243717 MS.gene92405:intron 20.0%
!!! TTTAATATTTTTGGAAACTC+TGG + chr8.4:60245468-60245487 MS.gene92405:intron 20.0%
! AAATGTTGTTAGATAGATTG+TGG + chr8.4:60244757-60244776 MS.gene92405:intron 25.0%
! ACATCATTATCTTGACTTAT+AGG - chr8.4:60244270-60244289 None:intergenic 25.0%
! AGATGAAAAAGAGTTAATCT+TGG + chr8.4:60243879-60243898 MS.gene92405:CDS 25.0%
! CATCATTACTTCAGAAAAAA+TGG - chr8.4:60244390-60244409 None:intergenic 25.0%
! GAACATCATTATAGTAATTG+AGG - chr8.4:60245265-60245284 None:intergenic 25.0%
! GGTTATTGGAAAATTTATGT+AGG + chr8.4:60243847-60243866 MS.gene92405:intron 25.0%
! TCTCAAAAATGAAAAGTCAA+TGG - chr8.4:60244043-60244062 None:intergenic 25.0%
! TGAATGAATAAACAACATCT+TGG - chr8.4:60245287-60245306 None:intergenic 25.0%
! TGGAAAATTTATGTAGGAAT+TGG + chr8.4:60243853-60243872 MS.gene92405:intron 25.0%
! TTATAAGGGATTCTATTGTT+GGG + chr8.4:60246081-60246100 MS.gene92405:CDS 25.0%
! TTGAATTTGTGAAGCAAATT+AGG - chr8.4:60244914-60244933 None:intergenic 25.0%
! TTTAATATCGCTCTTAAATG+TGG + chr8.4:60245361-60245380 MS.gene92405:CDS 25.0%
!! ATTCCAAACATTTTCTGAAT+GGG - chr8.4:60246029-60246048 None:intergenic 25.0%
!!! TTAATGTTGATGATGGTTAT+TGG + chr8.4:60243833-60243852 MS.gene92405:intron 25.0%
!!! TTTTAACTTATCTACAGTTC+TGG - chr8.4:60245563-60245582 None:intergenic 25.0%
GAAAGATACATGATATGTGT+CGG - chr8.4:60245826-60245845 None:intergenic 30.0%
GACATAAAAACTTGTGGTAA+AGG + chr8.4:60245335-60245354 MS.gene92405:CDS 30.0%
GACTTATAGGTGTAAAATTG+AGG - chr8.4:60244257-60244276 None:intergenic 30.0%
GTTATAAGGGATTCTATTGT+TGG + chr8.4:60246080-60246099 MS.gene92405:CDS 30.0%
TAATAAAGTGACAGTAAGCT+TGG + chr8.4:60245000-60245019 MS.gene92405:CDS 30.0%
TAGATGATGATCACTTTGTT+TGG + chr8.4:60245077-60245096 MS.gene92405:CDS 30.0%
TGAATCACAAAGATTTGTAG+AGG + chr8.4:60243679-60243698 MS.gene92405:intron 30.0%
TTACAGGACATAAAAACTTG+TGG + chr8.4:60245329-60245348 MS.gene92405:intron 30.0%
TTACCCATTCAGAAAATGTT+TGG + chr8.4:60246023-60246042 MS.gene92405:intron 30.0%
TTATCTAAGTTTGTCTAGCT+AGG + chr8.4:60244727-60244746 MS.gene92405:intron 30.0%
! ATTTGGGTGATTTTCTTTAC+AGG + chr8.4:60245313-60245332 MS.gene92405:intron 30.0%
! GAGATTGTTAATGTTGATGA+TGG + chr8.4:60243826-60243845 MS.gene92405:intron 30.0%
! GATTCCAAACATTTTCTGAA+TGG - chr8.4:60246030-60246049 None:intergenic 30.0%
!! AAAGAGTTAATCTTGGTGTT+AGG + chr8.4:60243886-60243905 MS.gene92405:CDS 30.0%
!! AGTGTTATGCAGATTTTAGT+GGG + chr8.4:60244956-60244975 MS.gene92405:intron 30.0%
!! ATTTTTGTATCGATGGTTGT+TGG + chr8.4:60243793-60243812 MS.gene92405:intron 30.0%
!!! ATTTTTTGCTGACTTTGCTA+GGG + chr8.4:60246249-60246268 MS.gene92405:CDS 30.0%
!!! TATGCTGATTTTTGTATCGA+TGG + chr8.4:60243786-60243805 MS.gene92405:intron 30.0%
AAAGAGAAAGAGAGAAACCT+TGG - chr8.4:60243487-60243506 None:intergenic 35.0%
AAAGGGATGCAAAATTTGCA+AGG + chr8.4:60244492-60244511 MS.gene92405:CDS 35.0%
AACAGAATGCTATAGTTCTC+TGG + chr8.4:60244453-60244472 MS.gene92405:intron 35.0%
AAGGGATGCAAAATTTGCAA+GGG + chr8.4:60244493-60244512 MS.gene92405:CDS 35.0%
CATTGAAGGTTAAGAGGAAA+GGG + chr8.4:60244519-60244538 MS.gene92405:CDS 35.0%
GACATAGAAATGCTCAACAA+AGG + chr8.4:60243908-60243927 MS.gene92405:CDS 35.0%
GCTACGCATACTAATTGTAT+AGG + chr8.4:60245724-60245743 MS.gene92405:intron 35.0%
TACCTTTGCGAAATGTTTCT+CGG - chr8.4:60245408-60245427 None:intergenic 35.0%
TAGAAATGCTCAACAAAGGA+AGG + chr8.4:60243912-60243931 MS.gene92405:CDS 35.0%
TAGAATCCCTTATAACCTGT+TGG - chr8.4:60246076-60246095 None:intergenic 35.0%
TATGCACCAACAGGTTATAA+GGG + chr8.4:60246067-60246086 MS.gene92405:intron 35.0%
! GAGTGTTATGCAGATTTTAG+TGG + chr8.4:60244955-60244974 MS.gene92405:intron 35.0%
! TCTAAAATCACCAGAGAGTT+CGG - chr8.4:60243995-60244014 None:intergenic 35.0%
!! AGTCTTGATGATGATGTCAT+TGG + chr8.4:60246203-60246222 MS.gene92405:CDS 35.0%
!! TGTCACTTTATTATGCCTGT+AGG - chr8.4:60244993-60245012 None:intergenic 35.0%
!!! GATTTTTTGCTGACTTTGCT+AGG + chr8.4:60246248-60246267 MS.gene92405:CDS 35.0%
ACCTTTGCGAAATGTTTCTC+GGG - chr8.4:60245407-60245426 None:intergenic 40.0%
ATTCACTTCTATGCACCAAC+AGG + chr8.4:60246058-60246077 MS.gene92405:intron 40.0%
ATTTGCAAGGGATGCATTGA+AGG + chr8.4:60244505-60244524 MS.gene92405:CDS 40.0%
CATCCGTTTACCAACCATTT+CGG + chr8.4:60245612-60245631 MS.gene92405:intron 40.0%
CGCTCTTAAATGTGGTAGTA+TGG + chr8.4:60245369-60245388 MS.gene92405:CDS 40.0%
CGTCTTAGAAATGCTTTCCA+AGG + chr8.4:60243467-60243486 MS.gene92405:intron 40.0%
CTATGCACCAACAGGTTATA+AGG + chr8.4:60246066-60246085 MS.gene92405:intron 40.0%
CTATGGCAAAACATTGGAAG+AGG + chr8.4:60245159-60245178 MS.gene92405:CDS 40.0%
CTGACTGCCATCAAATGATT+TGG - chr8.4:60244590-60244609 None:intergenic 40.0%
GCAACTCTATGGCAAAACAT+TGG + chr8.4:60245153-60245172 MS.gene92405:CDS 40.0%
GCATTGAAGGTTAAGAGGAA+AGG + chr8.4:60244518-60244537 MS.gene92405:CDS 40.0%
GTCGAGTCCAAATCATTTGA+TGG + chr8.4:60244580-60244599 MS.gene92405:CDS 40.0%
TAAAGTGACAGTAAGCTTGG+AGG + chr8.4:60245003-60245022 MS.gene92405:CDS 40.0%
TCTCTTTGATTGCTCACTTG+AGG + chr8.4:60244625-60244644 MS.gene92405:CDS 40.0%
TGATGTCATTGGAGATACCT+CGG + chr8.4:60246214-60246233 MS.gene92405:CDS 40.0%
TGTAGGATGCAAGAGTTTCA+CGG - chr8.4:60245132-60245151 None:intergenic 40.0%
TTAGGAGAAGGAACAATCTC+TGG - chr8.4:60243434-60243453 None:intergenic 40.0%
TTGAAAACTCGACTCCGAAA+TGG - chr8.4:60245629-60245648 None:intergenic 40.0%
TTGTTTGGATACTTAGCACC+AGG + chr8.4:60245092-60245111 MS.gene92405:CDS 40.0%
TTTGCCATAGAGTTGCTTGT+AGG - chr8.4:60245149-60245168 None:intergenic 40.0%
! AATTTTGCATCCCTTTCTGC+TGG - chr8.4:60244488-60244507 None:intergenic 40.0%
! CAAATCATTTGATGGCAGTC+AGG + chr8.4:60244588-60244607 MS.gene92405:CDS 40.0%
! TTTCGGAGTCGAGTTTTCAA+AGG + chr8.4:60245629-60245648 MS.gene92405:intron 40.0%
!! ACTCCGAAATGGTTGGTAAA+CGG - chr8.4:60245618-60245637 None:intergenic 40.0%
!! CATGTCCTTACTTTTGTGAG+TGG - chr8.4:60244677-60244696 None:intergenic 40.0%
AAAATCCATGTAGTCACCCG+AGG - chr8.4:60246234-60246253 None:intergenic 45.0%
AAACTCGACTCCGAAATGGT+TGG - chr8.4:60245625-60245644 None:intergenic 45.0%
AAGCTTGGAGGTTGCAAAGT+CGG + chr8.4:60245015-60245034 MS.gene92405:CDS 45.0%
ACGGATCTGGAAAACATTCC+TGG - chr8.4:60245113-60245132 None:intergenic 45.0%
AGACGTTCAGTGTCTGTGTT+AGG - chr8.4:60243452-60243471 None:intergenic 45.0%
AGGGATTCTATTGTTGGGCT+AGG + chr8.4:60246086-60246105 MS.gene92405:CDS 45.0%
ATGCAAGAGTTTCACGGATC+TGG - chr8.4:60245126-60245145 None:intergenic 45.0%
CCTTTGCGAAATGTTTCTCG+GGG - chr8.4:60245406-60245425 None:intergenic 45.0%
CTCAGCCACTCACAAAAGTA+AGG + chr8.4:60244669-60244688 MS.gene92405:intron 45.0%
CTTTGATTGCTCACTTGAGG+AGG + chr8.4:60244628-60244647 MS.gene92405:CDS 45.0%
GACTTGTGATCCAGCAGAAA+GGG + chr8.4:60244475-60244494 MS.gene92405:CDS 45.0%
GATCAAACAGCTGCAGATTC+TGG + chr8.4:60244541-60244560 MS.gene92405:CDS 45.0%
GATGTCATTGGAGATACCTC+GGG + chr8.4:60246215-60246234 MS.gene92405:CDS 45.0%
GCATCCTACAAGCAACTCTA+TGG + chr8.4:60245142-60245161 MS.gene92405:CDS 45.0%
GGGATGCATTGAAGGTTAAG+AGG + chr8.4:60244513-60244532 MS.gene92405:CDS 45.0%
TCAGTGTCTGTGTTAGGAGA+AGG - chr8.4:60243446-60243465 None:intergenic 45.0%
!! TTGCTGACTTTGCTAGGGAA+GGG + chr8.4:60246254-60246273 MS.gene92405:CDS 45.0%
!! TTTGCTGACTTTGCTAGGGA+AGG + chr8.4:60246253-60246272 MS.gene92405:CDS 45.0%
!!! TATAAGTTTTTTAATATTTT+TGG + chr8.4:60245459-60245478 MS.gene92405:intron 5.0%
!!! TTTTTTATGTTTTTATTAAT+GGG + chr8.4:60243613-60243632 MS.gene92405:intron 5.0%
AGATACCTCGGGTGACTACA+TGG + chr8.4:60246226-60246245 MS.gene92405:CDS 50.0%
CCCCGAGAAACATTTCGCAA+AGG + chr8.4:60245403-60245422 MS.gene92405:CDS 50.0%
CGTCTTCAATCCGAACTCTC+TGG + chr8.4:60243982-60244001 MS.gene92405:CDS 50.0%
GGACTTGTGATCCAGCAGAA+AGG + chr8.4:60244474-60244493 MS.gene92405:CDS 50.0%
TGGGCTTAGTAAGCTCCTAC+AGG + chr8.4:60244975-60244994 MS.gene92405:CDS 50.0%
TGTGAGTGGCTGAGAAACTG+AGG - chr8.4:60244663-60244682 None:intergenic 50.0%
TTGCTAGGGAAGGGACCAAA+GGG + chr8.4:60246263-60246282 MS.gene92405:CDS 50.0%
TTTGCTAGGGAAGGGACCAA+AGG + chr8.4:60246262-60246281 MS.gene92405:CDS 50.0%
! TCATTTGATGGCAGTCAGGC+AGG + chr8.4:60244592-60244611 MS.gene92405:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 60243413 60246292 60243413 ID=MS.gene92405
chr8.4 mRNA 60243413 60246292 60243413 ID=MS.gene92405.t1;Parent=MS.gene92405
chr8.4 exon 60243413 60243467 60243413 ID=MS.gene92405.t1.exon1;Parent=MS.gene92405.t1
chr8.4 CDS 60243413 60243467 60243413 ID=cds.MS.gene92405.t1;Parent=MS.gene92405.t1
chr8.4 exon 60243869 60244014 60243869 ID=MS.gene92405.t1.exon2;Parent=MS.gene92405.t1
chr8.4 CDS 60243869 60244014 60243869 ID=cds.MS.gene92405.t1;Parent=MS.gene92405.t1
chr8.4 exon 60244458 60244685 60244458 ID=MS.gene92405.t1.exon3;Parent=MS.gene92405.t1
chr8.4 CDS 60244458 60244685 60244458 ID=cds.MS.gene92405.t1;Parent=MS.gene92405.t1
chr8.4 exon 60244968 60245180 60244968 ID=MS.gene92405.t1.exon4;Parent=MS.gene92405.t1
chr8.4 CDS 60244968 60245180 60244968 ID=cds.MS.gene92405.t1;Parent=MS.gene92405.t1
chr8.4 exon 60245335 60245424 60245335 ID=MS.gene92405.t1.exon5;Parent=MS.gene92405.t1
chr8.4 CDS 60245335 60245424 60245335 ID=cds.MS.gene92405.t1;Parent=MS.gene92405.t1
chr8.4 exon 60246080 60246292 60246080 ID=MS.gene92405.t1.exon6;Parent=MS.gene92405.t1
chr8.4 CDS 60246080 60246292 60246080 ID=cds.MS.gene92405.t1;Parent=MS.gene92405.t1
Gene Sequence

>MS.gene92405

ATGGCTTCATTGAAACTACCAGAGATTGTTCCTTCTCCTAACACAGACACTGAACGTCTTAGAAATGCTTTCCAAGGTTTCTCTCTTTCTCTTTCTCTCTCTAATGCTATGAAAGTTTTTTAAAAAAAAAACTTTTTTTTTTAATGATGTTAAGTATTTTTCTGAATTTGTGTAGCGAGAGTTATGTTAAAAAACTGAACTTTTTTATGTTTTTATTAATGGGTTTTTTCAGAAACGTTACTTTATAATATGCATGTTTGTAAAATTGAATCACAAAGATTTGTAGAGGAATTTTTTTTATTCATTGGACAACATTGAAAGTACTGTTATGATTTTGTCATTTTATATCTTTTTTGTATTCTTTTGGTTCATTTATGCTGATTTTTGTATCGATGGTTGTTGGTATTAGAAATGAGATTGTTAATGTTGATGATGGTTATTGGAAAATTTATGTAGGAATTGGAACAGATGAAAAAGAGTTAATCTTGGTGTTAGGACATAGAAATGCTCAACAAAGGAAGGAAATCAGAGAAACTTATCAGCAACGTTACAATGAATCGCTTCTTGATCGTCTTCAATCCGAACTCTCTGGTGATTTTAGAGTATGTCATCATGTCATTCTTGCTTCCATTGACTTTTCATTTTTGAGAAACAATTCTTCAATGTCGTATTTTATCCGTATATATTATTGAAATCAACTGAAATTATGTCATTTTCAGTGATTGCTTGTGCCAATTTGAAATTCTAGTTTTTTTGTTACAAAATTGGTCAATCATGTGTCCTATTACTCTATCTGTATTTAAATTTTGAGCTAATTTGATTTGAGCATTACAAATATAAACCTCAATTTTACACCTATAAGTCAAGATAATGATGTTTGTCATAACAATTTTATAAGCTTTTGGTACATTATTTCTTATATCTAAGGAAAAGTTCATTTTATCGTCGTATTTCAATATGATTTTCGAAGCGTGCCATTTTTTCTGAAGTAATGATGCGATCTACAATGCAATATCATGACATAGAATAGTGTGATATGTAACAGAATGCTATAGTTCTCTGGACTTGTGATCCAGCAGAAAGGGATGCAAAATTTGCAAGGGATGCATTGAAGGTTAAGAGGAAAGGGATCAAACAGCTGCAGATTCTGGTTGAAATAGCATGCGCGTCGAGTCCAAATCATTTGATGGCAGTCAGGCAGGCTTACTGCTCTCTCTTTGATTGCTCACTTGAGGAGGACATTATAGCCTCAGTTTCTCAGCCACTCACAAAAGTAAGGACATGAAGTTTCTTATTCGCATATAAAAGAAACTCTTATCTAAGTTTGTCTAGCTAGGTTCAAGTAAATGTTGTTAGATAGATTGTGGCTAAAAGAAAATGTTAGACCTGTTTTCAAGTATTTTAAAAGAAAATATTGAGGGGAATGTTGAGTCTATATTCTCATATGTTTATTAGTTGAACTATAAATGAAAACGGAAAACATGAAAAGAAAACGAGTCCTAATTTGCTTCACAAATTCAATGTTCGTCAAACTTATGATGTGAGTGTTATGCAGATTTTAGTGGGCTTAGTAAGCTCCTACAGGCATAATAAAGTGACAGTAAGCTTGGAGGTTGCAAAGTCGGAAGCAGAAAAACTACATGAAGCAATCAACAATAAGAAATTAGATGATGATCACTTTGTTTGGATACTTAGCACCAGGAATGTTTTCCAGATCCGTGAAACTCTTGCATCCTACAAGCAACTCTATGGCAAAACATTGGAAGAGGTTTTGTTTAACGATTTCAATTTTAATGTCAACTAGTAATTTAAATCATGTCACTCATTCGAAATGTAGTTAACAGAAAAGCCTCAATTACTATAATGATGTTCCAAGATGTTGTTTATTCATTCACTATGAATTTGGGTGATTTTCTTTACAGGACATAAAAACTTGTGGTAAAGGTGATTTAATATCGCTCTTAAATGTGGTAGTATGGTGTATAGAATGCCCCGAGAAACATTTCGCAAAGGTACTTGTCAATTATCTTCTACTCAAGACAAGTTTATAAGTTTTTTAATATTTTTGGAAACTCTGGTTTATAAGTTAATACTACATTATTAGAACTCACTGATCAATTTTCAAGATATATCTCTAACTCGCTTTTCCAGAACTGTAGATAAGTTAAAAAGAAAAGAATCTGTCTTTCTTTTTCTGTTCATCCGTTTACCAACCATTTCGGAGTCGAGTTTTCAAAGGCCAAGTTTATCAATATTGTAAATGATACATTGTACATACTATTGATTATTTCTAATTTTTAAGATTAGATATGCTACGCATACTAATTGTATAGGTTATTGATAGCTCGCATTGAGAATTTAGCTTGTTTGCTTGAGATTCTTCTCTATTCAAAGACGATAGCTGCATACTCCGACACATATCATGTATCTTTCGTTCGATCTTCTAATGAAAATTTCATTAAGGTGGTTTGAAAGATCAAATTTTGACATCAATTCAGTCGTTTTATATGACTATTTTGATATCTTTATAGAAGTACCAACATTTTTGCATTTCATTTTTAAAATATCAAAGTATCCATTTACTGTAATTTCTTTCAAGCTATATCAAGATTACCCATTCAGAAAATGTTTGGAATCTCATTTCGATTCACTTCTATGCACCAACAGGTTATAAGGGATTCTATTGTTGGGCTAGGAACTGATGAAGATTCTCTTAACAGAGCTATTGTAACTCGAGCTGAAATCGACTTGTTGAAAGTGAGATTCGAGTATGCTAACATGTACAAATCAAGTCTTGATGATGATGTCATTGGAGATACCTCGGGTGACTACATGGATTTTTTGCTGACTTTGCTAGGGAAGGGACCAAAGGGATGCTAA
Protein sequence

>MS.gene92405.t1

MASLKLPEIVPSPNTDTERIGTDEKELILVLGHRNAQQRKEIRETYQQRYNESLLDRLQSELSGDFRNAIVLWTCDPAERDAKFARDALKVKRKGIKQLQILVEIACASSPNHLMAVRQAYCSLFDCSLEEDIIASVSQPLTKILVGLVSSYRHNKVTVSLEVAKSEAEKLHEAINNKKLDDDHFVWILSTRNVFQIRETLASYKQLYGKTLEEDIKTCGKGDLISLLNVVVWCIECPEKHFAKVIRDSIVGLGTDEDSLNRAIVTRAEIDLLKVRFEYANMYKSSLDDDVIGDTSGDYMDFLLTLLGKGPKGC