AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059027

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059027.t1 MTR_1g069275 97.150 421 11 1 1 420 1 421 0.0 838
MS.gene059027.t1 MTR_7g099330 78.147 421 86 3 1 420 1 416 0.0 689
MS.gene059027.t1 MTR_1g046230 72.048 415 112 1 10 420 9 423 0.0 622
MS.gene059027.t1 MTR_7g099160 77.508 329 69 2 93 420 213 537 0.0 529
MS.gene059027.t1 MTR_1g008380 63.496 389 140 2 18 404 35 423 0.0 521
MS.gene059027.t1 MTR_3g109520 60.050 403 158 2 4 403 18 420 1.17e-180 510
MS.gene059027.t1 MTR_1g011630 59.162 382 154 1 18 399 9 388 3.19e-178 503
MS.gene059027.t1 MTR_6g086870 59.194 397 156 3 9 399 4 400 4.13e-178 503
MS.gene059027.t1 MTR_7g107710 47.105 380 192 4 26 401 7 381 1.44e-122 361
MS.gene059027.t1 MTR_8g432640 47.733 375 187 4 26 396 7 376 2.93e-120 355
MS.gene059027.t1 MTR_5g033260 46.842 380 187 5 26 398 7 378 1.07e-119 353
MS.gene059027.t1 MTR_7g107690 44.063 379 203 4 26 400 9 382 3.17e-119 352
MS.gene059027.t1 MTR_3g088925 44.267 375 198 5 26 395 8 376 9.95e-117 346
MS.gene059027.t1 MTR_3g088945 44.000 375 199 5 26 395 8 376 1.06e-115 343
MS.gene059027.t1 MTR_4g051642 45.333 375 193 6 26 395 8 375 4.44e-114 340
MS.gene059027.t1 MTR_3g088955 43.467 375 201 5 26 395 8 376 7.57e-114 339
MS.gene059027.t1 MTR_3g096030 38.068 176 83 2 26 199 7 158 9.42e-37 132
MS.gene059027.t1 MTR_2g450310 29.487 234 127 9 27 223 7 239 2.95e-15 77.8
MS.gene059027.t1 MTR_2g450510 28.205 234 130 8 27 223 7 239 4.72e-14 74.3
MS.gene059027.t1 MTR_3g437580 24.017 229 139 5 27 220 15 243 5.44e-14 73.2
MS.gene059027.t1 MTR_8g037910 22.488 209 132 3 27 208 6 211 6.61e-14 72.0
MS.gene059027.t1 MTR_3g437580 24.017 229 139 5 27 220 15 243 8.33e-14 73.2
MS.gene059027.t1 MTR_8g037910 22.488 209 132 3 27 208 6 211 9.07e-14 72.4
MS.gene059027.t1 MTR_8g037910 22.488 209 132 3 27 208 6 211 1.40e-13 72.4
MS.gene059027.t1 MTR_2g044140 26.757 370 207 14 27 336 16 381 5.91e-12 67.8
MS.gene059027.t1 MTR_2g043850 25.806 372 208 16 27 336 16 381 3.64e-11 65.5
MS.gene059027.t1 MTR_2g020630 25.417 240 138 9 27 227 16 253 4.14e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059027.t1 AT4G28720 71.698 424 115 2 1 419 1 424 0.0 644
MS.gene059027.t1 AT1G04180 71.360 419 119 1 1 418 1 419 0.0 626
MS.gene059027.t1 AT5G43890 69.431 422 125 2 1 418 1 422 0.0 614
MS.gene059027.t1 AT1G04610 69.212 419 124 1 7 420 19 437 0.0 612
MS.gene059027.t1 AT2G33230 69.082 414 122 2 12 420 19 431 0.0 581
MS.gene059027.t1 AT5G25620 60.100 401 157 2 9 406 16 416 2.25e-178 504
MS.gene059027.t1 AT5G25620 60.250 400 155 3 6 401 12 411 1.80e-177 502
MS.gene059027.t1 AT5G11320 59.843 381 149 1 20 400 12 388 3.89e-172 488
MS.gene059027.t1 AT4G32540 55.335 403 174 3 16 417 12 409 1.19e-171 487
MS.gene059027.t1 AT4G13260 60.789 380 146 2 18 394 21 400 2.83e-171 486
MS.gene059027.t1 AT4G32540 55.309 405 173 4 16 417 12 411 4.75e-170 483
MS.gene059027.t1 AT5G11320 59.202 326 129 1 20 345 12 333 2.35e-142 410
MS.gene059027.t1 AT4G13260 61.379 290 110 1 18 305 21 310 1.08e-131 381
MS.gene059027.t1 AT1G48910 47.215 377 188 5 26 397 6 376 1.31e-125 369
MS.gene059027.t1 AT1G21430 48.128 374 182 5 26 394 10 376 1.43e-122 361
MS.gene059027.t1 AT4G13260 58.131 289 118 2 109 394 9 297 1.40e-114 338
MS.gene059027.t1 AT1G62560 27.230 213 122 6 27 208 15 225 3.36e-19 89.4
MS.gene059027.t1 AT1G62560 27.230 213 122 6 27 208 15 225 7.79e-19 88.6
MS.gene059027.t1 AT5G45180 26.136 352 192 14 27 336 15 340 1.83e-17 84.7
MS.gene059027.t1 AT1G65860 25.561 223 138 4 27 221 15 237 4.66e-16 80.1
MS.gene059027.t1 AT1G12160 26.636 214 124 6 27 209 14 225 1.56e-15 78.6
MS.gene059027.t1 AT1G62620 25.926 243 138 8 27 234 15 250 2.89e-15 77.4
MS.gene059027.t1 AT5G07800 26.147 218 125 5 27 208 18 235 3.04e-15 77.8
MS.gene059027.t1 AT1G63370 25.000 240 144 7 27 234 15 250 5.06e-15 76.3
MS.gene059027.t1 AT1G62620 25.417 240 143 7 27 234 15 250 5.90e-15 77.0
MS.gene059027.t1 AT1G63370 25.000 240 144 7 27 234 15 250 1.09e-14 75.9
MS.gene059027.t1 AT5G61290 26.267 217 126 5 27 209 18 234 2.18e-14 75.1
MS.gene059027.t1 AT1G62540 24.880 209 129 3 41 221 29 237 4.22e-14 74.3
MS.gene059027.t1 AT1G62540 24.880 209 129 3 41 221 29 237 4.61e-14 73.9
MS.gene059027.t1 AT1G62540 24.880 209 129 3 41 221 29 237 4.86e-14 74.3
MS.gene059027.t1 AT1G12200 25.743 202 117 4 41 209 30 231 6.90e-14 73.2
MS.gene059027.t1 AT1G12200 25.743 202 117 4 41 209 30 231 1.93e-13 72.4
MS.gene059027.t1 AT1G12130 23.451 226 142 5 41 235 29 254 7.78e-13 70.5
MS.gene059027.t1 AT1G62570 22.967 209 133 3 41 221 29 237 2.18e-12 68.9
MS.gene059027.t1 AT1G12140 22.857 210 133 3 41 221 29 238 2.92e-12 68.6
MS.gene059027.t1 AT1G12140 22.857 210 133 3 41 221 29 238 2.92e-12 68.6

Find 88 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTCTTTGTTGGATATTC+AGG 0.151113 1.2:+52093871 None:intergenic
GTTGTTGGTTGTGGTAATTC+TGG 0.205623 1.2:-52093562 MS.gene059027:CDS
CTCAAGATATTGCTCAAGTT+TGG 0.269888 1.2:-52092338 MS.gene059027:CDS
ATCGTAGCAATGTCCCTTCT+TGG 0.282135 1.2:-52092843 MS.gene059027:CDS
TAGAAAAGTATGGTCTTAAA+AGG 0.282601 1.2:-52093056 MS.gene059027:CDS
CAATTTGTGCAATTTAGAAT+TGG 0.285416 1.2:+52092256 None:intergenic
GCTAAATATGATGAGACTAA+TGG 0.295504 1.2:-52093775 MS.gene059027:CDS
GGTCCTGCTCCTACTATTAT+AGG 0.301581 1.2:+52094066 None:intergenic
CATGCTTGTGATTATAAATC+AGG 0.314519 1.2:-52093613 MS.gene059027:CDS
AAAGGGTAAGACCCCTGTTT+TGG 0.316214 1.2:-52093001 MS.gene059027:CDS
TTACTCAACCCTTCAATATC+AGG 0.327104 1.2:+52093655 None:intergenic
GGCTGTGTTGAACTTGTTAA+TGG 0.328799 1.2:-52092907 MS.gene059027:CDS
TTTATGAACTGTTTCTTTGT+TGG 0.340000 1.2:+52093862 None:intergenic
CAGTTCCTTTCCAACCATTA+GGG 0.343203 1.2:+52092430 None:intergenic
TAGTCTCACATCGTTGCATT+TGG 0.343936 1.2:-52094098 MS.gene059027:CDS
AAGGCCATTGGAGGGTCCTT+TGG 0.345490 1.2:-52093037 MS.gene059027:CDS
ACAGTTCCTTTCCAACCATT+AGG 0.345992 1.2:+52092429 None:intergenic
CAATGTCCCTTCTTGGCTTA+AGG 0.348481 1.2:-52092836 MS.gene059027:intron
TGACTTTGGAAATCCATTCT+TGG 0.361539 1.2:+52092458 None:intergenic
CCAAAGTCACCATTCCCTAA+TGG 0.366181 1.2:-52092444 MS.gene059027:CDS
TTAGTAAACCTCTTAATTCC+AGG 0.368388 1.2:+52092931 None:intergenic
TCTTGTGGGAATGGAAAGTT+AGG 0.369111 1.2:+52093898 None:intergenic
CTGTTGGACTTTATGCTGTT+GGG 0.378354 1.2:-52092410 MS.gene059027:CDS
CATCGTTGCATTTGGGTAAA+CGG 0.385627 1.2:-52094090 MS.gene059027:CDS
AATTTGTGCAATTTAGAATT+GGG 0.388229 1.2:+52092257 None:intergenic
AAGGTGCCAATATCCAAAAC+AGG 0.397457 1.2:+52092988 None:intergenic
AGTCTCACATCGTTGCATTT+GGG 0.410240 1.2:-52094097 MS.gene059027:CDS
ATACTCAACTTCATTTCCAT+TGG 0.414194 1.2:+52093729 None:intergenic
TTTGGTAATATAGAAAAGTA+TGG 0.417326 1.2:-52093066 MS.gene059027:CDS
CTAAATATGATGAGACTAAT+GGG 0.418507 1.2:-52093774 MS.gene059027:CDS
TGATGAAATGGTTACCCGTT+TGG 0.423835 1.2:-52093128 MS.gene059027:CDS
AGGTCGCTTGTGGTTGCTAC+TGG 0.431631 1.2:-52093700 MS.gene059027:CDS
GATGCAGTTGTTCTTGCTAC+TGG 0.439635 1.2:-52092868 MS.gene059027:CDS
CCATTAGGGAATGGTGACTT+TGG 0.441458 1.2:+52092444 None:intergenic
ACAACAAGGAGTACCCTTCA+TGG 0.462402 1.2:-52094013 MS.gene059027:CDS
GTCTATTGTTCTTCGTAGCT+CGG 0.464587 1.2:-52093497 MS.gene059027:intron
TCAGGAAATTCTTGTGGGAA+TGG 0.465607 1.2:+52093889 None:intergenic
ATGAAGTTGAGTATATTTGT+AGG 0.466018 1.2:-52093720 MS.gene059027:CDS
GATATTCAGGAAATTCTTGT+GGG 0.470627 1.2:+52093884 None:intergenic
ACTGTTGGACTTTATGCTGT+TGG 0.470697 1.2:-52092411 MS.gene059027:CDS
ATAGTAGGAGCAGGACCATC+AGG 0.478379 1.2:-52094060 MS.gene059027:CDS
TGGCAGTTATGATGATGAAA+TGG 0.484448 1.2:-52093140 MS.gene059027:CDS
TTTCTCACCTTAAGCCAAGA+AGG 0.485907 1.2:+52092829 None:intergenic
TTTACAAAGAGAGGGCTCTC+TGG 0.487445 1.2:-52092387 MS.gene059027:CDS
AATCTTGTCAACTATCCAAA+CGG 0.487573 1.2:+52093113 None:intergenic
TGGGAAATCAACATTTGGAT+TGG 0.487762 1.2:-52093160 MS.gene059027:CDS
GGAATTAAGAGGTTTACTAA+TGG 0.490761 1.2:-52092928 MS.gene059027:CDS
ATCTTGTCAACTATCCAAAC+GGG 0.505639 1.2:+52093114 None:intergenic
TTGGAGCTTAAGAACACAAA+GGG 0.508263 1.2:-52093018 MS.gene059027:CDS
GTAAACGGTCCTATAATAGT+AGG 0.514321 1.2:-52094075 MS.gene059027:CDS
TGATCTTTCAAGCACCATGA+AGG 0.516098 1.2:+52093999 None:intergenic
AAGTTGTGCCTGGAATTAAG+AGG 0.518103 1.2:-52092939 MS.gene059027:CDS
GGATATTCAGGAAATTCTTG+TGG 0.520358 1.2:+52093883 None:intergenic
CCTTTCCAACCATTAGGGAA+TGG 0.520534 1.2:+52092435 None:intergenic
ACCTTGGAGAAAATTAGATC+TGG 0.532203 1.2:-52092970 MS.gene059027:CDS
GTATGGTCTTAAAAGGCCAT+TGG 0.535113 1.2:-52093049 MS.gene059027:CDS
TTCTCACCTTAAGCCAAGAA+GGG 0.539788 1.2:+52092830 None:intergenic
GCTGTTGGGTTTACAAAGAG+AGG 0.540106 1.2:-52092396 MS.gene059027:CDS
TTTGTGTTCTTAAGCTCCAA+AGG 0.541522 1.2:+52093021 None:intergenic
TGTGATTATAAATCAGGTGA+AGG 0.541872 1.2:-52093607 MS.gene059027:CDS
TTTCTTCTTCGTTATCGACT+AGG 0.550794 1.2:+52094121 None:intergenic
TGGTCTTAAAAGGCCATTGG+AGG 0.553703 1.2:-52093046 MS.gene059027:CDS
TGTGTTGTGCCTGATATTGA+AGG 0.554440 1.2:-52093664 MS.gene059027:CDS
GCTGCTGTTGCTAGACCTGA+TGG 0.555064 1.2:+52094045 None:intergenic
AATGGTTGGAAAGGAACTGT+TGG 0.555476 1.2:-52092426 MS.gene059027:CDS
AGTCACCATTCCCTAATGGT+TGG 0.557817 1.2:-52092440 MS.gene059027:CDS
GAACACACTCGTTGAATCGT+GGG 0.559010 1.2:+52093803 None:intergenic
CCATTCCCTAATGGTTGGAA+AGG 0.559102 1.2:-52092435 MS.gene059027:CDS
ATGATGAGACTAATGGGTTG+TGG 0.559573 1.2:-52093768 MS.gene059027:CDS
GGTCACATAAAAGTTGTGCC+TGG 0.561332 1.2:-52092949 MS.gene059027:CDS
TGGTTGTGGTAATTCTGGAA+TGG 0.564985 1.2:-52093557 MS.gene059027:CDS
CTGTTGGGTTTACAAAGAGA+GGG 0.568492 1.2:-52092395 MS.gene059027:CDS
TGAACACACTCGTTGAATCG+TGG 0.570661 1.2:+52093802 None:intergenic
GATCTTTCAAGCACCATGAA+GGG 0.575566 1.2:+52094000 None:intergenic
GATTTCGAAGTGTTTCGCGT+AGG 0.586522 1.2:+52093828 None:intergenic
GAAGGGTTGAGTAAATTCAA+AGG 0.589620 1.2:-52093646 MS.gene059027:CDS
AGCTCCAAAGGACCCTCCAA+TGG 0.594886 1.2:+52093033 None:intergenic
GGTCCTATAATAGTAGGAGC+AGG 0.599245 1.2:-52094069 MS.gene059027:CDS
AGAGTTGAGACTGTTTCCAA+TGG 0.605340 1.2:-52093745 MS.gene059027:CDS
GTATATTTGTAGGTCGCTTG+TGG 0.619170 1.2:-52093710 MS.gene059027:CDS
AGGTGCCAATATCCAAAACA+GGG 0.629075 1.2:+52092989 None:intergenic
GTGTTGTGCCTGATATTGAA+GGG 0.636186 1.2:-52093663 MS.gene059027:CDS
GGTCTTAAAAGGCCATTGGA+GGG 0.661835 1.2:-52093045 MS.gene059027:CDS
TTTGGAGCTTAAGAACACAA+AGG 0.673099 1.2:-52093019 MS.gene059027:CDS
GAGCTACGAAGAACAATAGA+CGG 0.679802 1.2:+52093499 None:intergenic
GCAGCATGTCTTAAACAACA+AGG 0.727523 1.2:-52094027 MS.gene059027:CDS
GGTGCCAATATCCAAAACAG+GGG 0.738091 1.2:+52092990 None:intergenic
CAAATTGCATAGCTTCACTG+TGG 0.767067 1.2:-52093978 MS.gene059027:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAAGAAAAATAATTTG+AGG - chr1.2:52093143-52093162 MS.gene059027:CDS 10.0%
!!! TTTTTTTTTTGTACTAATTA+AGG - chr1.2:52093175-52093194 MS.gene059027:CDS 10.0%
!!! GTTAATAACTAATCTTATTT+TGG - chr1.2:52093893-52093912 MS.gene059027:CDS 15.0%
!!! AGTATTTTACTATTTTTGTG+TGG - chr1.2:52093869-52093888 MS.gene059027:CDS 20.0%
!!! TTATGATTTTGTTTATTTCG+AGG - chr1.2:52093100-52093119 MS.gene059027:CDS 20.0%
!!! TTTGGTAATATAGAAAAGTA+TGG - chr1.2:52093341-52093360 MS.gene059027:intron 20.0%
! ATACTAATTCCATTTCCAAA+AGG + chr1.2:52093854-52093873 None:intergenic 25.0%
! ATATGATAAGACCCAAAAAA+AGG + chr1.2:52093750-52093769 None:intergenic 25.0%
! ATGAAGTTGAGTATATTTGT+AGG - chr1.2:52092687-52092706 MS.gene059027:intron 25.0%
! CTAAATATGATGAGACTAAT+GGG - chr1.2:52092633-52092652 MS.gene059027:intron 25.0%
! TACTAATTCCATTTCCAAAA+GGG + chr1.2:52093853-52093872 None:intergenic 25.0%
!! AATTAAGGTTGTTGTGTTTT+TGG - chr1.2:52093190-52093209 MS.gene059027:intron 25.0%
!! TAGAAAAGTATGGTCTTAAA+AGG - chr1.2:52093351-52093370 MS.gene059027:intron 25.0%
!! TTTATGAACTGTTTCTTTGT+TGG + chr1.2:52092548-52092567 None:intergenic 25.0%
!!! GTAGTTGACAGAATTTTTTA+GGG + chr1.2:52092490-52092509 None:intergenic 25.0%
!!! TGACAAGATTCTTTTGATTT+TGG - chr1.2:52093307-52093326 MS.gene059027:intron 25.0%
!!! TGGTTTTGAAACCTTTTTTT+GGG - chr1.2:52093736-52093755 MS.gene059027:CDS 25.0%
!!! TTGGTTTTGAAACCTTTTTT+TGG - chr1.2:52093735-52093754 MS.gene059027:CDS 25.0%
AATCTTGTCAACTATCCAAA+CGG + chr1.2:52093297-52093316 None:intergenic 30.0%
ACTAATTCCATTTCCAAAAG+GGG + chr1.2:52093852-52093871 None:intergenic 30.0%
ATACTCAACTTCATTTCCAT+TGG + chr1.2:52092681-52092700 None:intergenic 30.0%
CATGCTTGTGATTATAAATC+AGG - chr1.2:52092794-52092813 MS.gene059027:intron 30.0%
GATATTCAGGAAATTCTTGT+GGG + chr1.2:52092526-52092545 None:intergenic 30.0%
GCTAAATATGATGAGACTAA+TGG - chr1.2:52092632-52092651 MS.gene059027:intron 30.0%
GGAATTAAGAGGTTTACTAA+TGG - chr1.2:52093479-52093498 MS.gene059027:intron 30.0%
TGCAAATTTGATGTTTGGTT+CGG - chr1.2:52093699-52093718 MS.gene059027:CDS 30.0%
TGTGATTATAAATCAGGTGA+AGG - chr1.2:52092800-52092819 MS.gene059027:intron 30.0%
TGTTTCTTTGTTGGATATTC+AGG + chr1.2:52092539-52092558 None:intergenic 30.0%
TTAGTAAACCTCTTAATTCC+AGG + chr1.2:52093479-52093498 None:intergenic 30.0%
! TTTTGCCTAGAGAAGTTTTT+GGG - chr1.2:52093228-52093247 MS.gene059027:intron 30.0%
! TTTTTACCTTTTTCCCCTTT+TGG - chr1.2:52093836-52093855 MS.gene059027:CDS 30.0%
!! GTTTTTGGGAAATCAACATT+TGG - chr1.2:52093242-52093261 MS.gene059027:intron 30.0%
!! TTTGGTTCATTTTTGTGTGT+AGG - chr1.2:52093911-52093930 MS.gene059027:CDS 30.0%
!! TTTTAGAGGGAAGAAAGTTT+TGG - chr1.2:52092823-52092842 MS.gene059027:intron 30.0%
!!! AGATTCTTTTGATTTTGGCT+TGG - chr1.2:52093312-52093331 MS.gene059027:intron 30.0%
!!! GGTAGTTGACAGAATTTTTT+AGG + chr1.2:52092491-52092510 None:intergenic 30.0%
ACAGTTGAACCATGATTCAT+AGG - chr1.2:52093673-52093692 MS.gene059027:CDS 35.0%
ACCTTGGAGAAAATTAGATC+TGG - chr1.2:52093437-52093456 MS.gene059027:intron 35.0%
ATCTTGTCAACTATCCAAAC+GGG + chr1.2:52093296-52093315 None:intergenic 35.0%
CTCAAGATATTGCTCAAGTT+TGG - chr1.2:52094069-52094088 MS.gene059027:CDS 35.0%
GAAGGGTTGAGTAAATTCAA+AGG - chr1.2:52092761-52092780 MS.gene059027:intron 35.0%
GATTTCCCAAAAACTTCTCT+AGG + chr1.2:52093236-52093255 None:intergenic 35.0%
GGATATTCAGGAAATTCTTG+TGG + chr1.2:52092527-52092546 None:intergenic 35.0%
GGTCTTGCAAATTTGATGTT+TGG - chr1.2:52093694-52093713 MS.gene059027:CDS 35.0%
GTAAACGGTCCTATAATAGT+AGG - chr1.2:52092332-52092351 MS.gene059027:CDS 35.0%
TGGGAAATCAACATTTGGAT+TGG - chr1.2:52093247-52093266 MS.gene059027:intron 35.0%
TTACTCAACCCTTCAATATC+AGG + chr1.2:52092755-52092774 None:intergenic 35.0%
TTGGAGCTTAAGAACACAAA+GGG - chr1.2:52093389-52093408 MS.gene059027:intron 35.0%
TTTGCAAGACCTATGAATCA+TGG + chr1.2:52093685-52093704 None:intergenic 35.0%
TTTGTGTTCTTAAGCTCCAA+AGG + chr1.2:52093389-52093408 None:intergenic 35.0%
! ACCAGATCTAATTTTCTCCA+AGG + chr1.2:52093441-52093460 None:intergenic 35.0%
! GGTGAATTTTTCTCCAAGAA+TGG - chr1.2:52093936-52093955 MS.gene059027:CDS 35.0%
! GTTTTGCCTAGAGAAGTTTT+TGG - chr1.2:52093227-52093246 MS.gene059027:intron 35.0%
! TGACTTTGGAAATCCATTCT+TGG + chr1.2:52093952-52093971 None:intergenic 35.0%
! TGGCAGTTATGATGATGAAA+TGG - chr1.2:52093267-52093286 MS.gene059027:intron 35.0%
! TTTCTTCTTCGTTATCGACT+AGG + chr1.2:52092289-52092308 None:intergenic 35.0%
! TTTGGAGCTTAAGAACACAA+AGG - chr1.2:52093388-52093407 MS.gene059027:intron 35.0%
!! AAATCAGGTGAAGGTTTTAG+AGG - chr1.2:52092809-52092828 MS.gene059027:intron 35.0%
!! GGTCGAAGATTTTTAAGCTT+GGG - chr1.2:52093071-52093090 MS.gene059027:CDS 35.0%
!! GTCGAAGATTTTTAAGCTTG+GGG - chr1.2:52093072-52093091 MS.gene059027:CDS 35.0%
!! GTTCATTTTTGTGTGTAGGA+AGG - chr1.2:52093915-52093934 MS.gene059027:CDS 35.0%
!! TGGTCGAAGATTTTTAAGCT+TGG - chr1.2:52093070-52093089 MS.gene059027:CDS 35.0%
!! TTCAACTGTTTTGAACTGCT+CGG + chr1.2:52093662-52093681 None:intergenic 35.0%
!! TTGCTCTGAAAGAACTAATG+TGG - chr1.2:52093050-52093069 MS.gene059027:CDS 35.0%
!!! AAAGTTTTGGTTGTTGGTTG+TGG - chr1.2:52092836-52092855 MS.gene059027:intron 35.0%
!!! AATCAGGTGAAGGTTTTAGA+GGG - chr1.2:52092810-52092829 MS.gene059027:intron 35.0%
!!! ATTTTGGCTTGGTTAGTGTT+TGG - chr1.2:52093323-52093342 MS.gene059027:intron 35.0%
!!! GTTGACAGAATTTTTTAGGG+AGG + chr1.2:52092487-52092506 None:intergenic 35.0%
!!! TGTTGTGTTTTTGGTTGAAC+AGG - chr1.2:52093199-52093218 MS.gene059027:intron 35.0%
AAGTTGTGCCTGGAATTAAG+AGG - chr1.2:52093468-52093487 MS.gene059027:intron 40.0%
AATGGTTGGAAAGGAACTGT+TGG - chr1.2:52093981-52094000 MS.gene059027:CDS 40.0%
ACAGTTCCTTTCCAACCATT+AGG + chr1.2:52093981-52094000 None:intergenic 40.0%
ACTGTTGGACTTTATGCTGT+TGG - chr1.2:52093996-52094015 MS.gene059027:CDS 40.0%
AGAGTTGAGACTGTTTCCAA+TGG - chr1.2:52092662-52092681 MS.gene059027:intron 40.0%
AGGTGCCAATATCCAAAACA+GGG + chr1.2:52093421-52093440 None:intergenic 40.0%
AGTCTCACATCGTTGCATTT+GGG - chr1.2:52092310-52092329 MS.gene059027:CDS 40.0%
CAAATTGCATAGCTTCACTG+TGG - chr1.2:52092429-52092448 MS.gene059027:CDS 40.0%
CAGTTCCTTTCCAACCATTA+GGG + chr1.2:52093980-52093999 None:intergenic 40.0%
CATCGTTGCATTTGGGTAAA+CGG - chr1.2:52092317-52092336 MS.gene059027:CDS 40.0%
CCATTTCCAAAAGGGGAAAA+AGG + chr1.2:52093845-52093864 None:intergenic 40.0%
CTGTTGGACTTTATGCTGTT+GGG - chr1.2:52093997-52094016 MS.gene059027:CDS 40.0%
CTGTTGGGTTTACAAAGAGA+GGG - chr1.2:52094012-52094031 MS.gene059027:CDS 40.0%
GAGCTACGAAGAACAATAGA+CGG + chr1.2:52092911-52092930 None:intergenic 40.0%
GATCTTTCAAGCACCATGAA+GGG + chr1.2:52092410-52092429 None:intergenic 40.0%
GCAGCATGTCTTAAACAACA+AGG - chr1.2:52092380-52092399 MS.gene059027:CDS 40.0%
GTATATTTGTAGGTCGCTTG+TGG - chr1.2:52092697-52092716 MS.gene059027:intron 40.0%
GTCTATTGTTCTTCGTAGCT+CGG - chr1.2:52092910-52092929 MS.gene059027:CDS 40.0%
GTGTTGTGCCTGATATTGAA+GGG - chr1.2:52092744-52092763 MS.gene059027:intron 40.0%
TAGTCTCACATCGTTGCATT+TGG - chr1.2:52092309-52092328 MS.gene059027:CDS 40.0%
TCAGGAAATTCTTGTGGGAA+TGG + chr1.2:52092521-52092540 None:intergenic 40.0%
TCTTGTGGGAATGGAAAGTT+AGG + chr1.2:52092512-52092531 None:intergenic 40.0%
TGATCTTTCAAGCACCATGA+AGG + chr1.2:52092411-52092430 None:intergenic 40.0%
TGATGAAATGGTTACCCGTT+TGG - chr1.2:52093279-52093298 MS.gene059027:intron 40.0%
TGTGTTGTGCCTGATATTGA+AGG - chr1.2:52092743-52092762 MS.gene059027:intron 40.0%
TTCTCACCTTAAGCCAAGAA+GGG + chr1.2:52093580-52093599 None:intergenic 40.0%
TTTCTCACCTTAAGCCAAGA+AGG + chr1.2:52093581-52093600 None:intergenic 40.0%
! AAGGTGCCAATATCCAAAAC+AGG + chr1.2:52093422-52093441 None:intergenic 40.0%
! GTATGGTCTTAAAAGGCCAT+TGG - chr1.2:52093358-52093377 MS.gene059027:intron 40.0%
! GTTGTTGGTTGTGGTAATTC+TGG - chr1.2:52092845-52092864 MS.gene059027:CDS 40.0%
! TGGTTGTGGTAATTCTGGAA+TGG - chr1.2:52092850-52092869 MS.gene059027:CDS 40.0%
!! AAGACCCCTGTTTTGGATAT+TGG - chr1.2:52093413-52093432 MS.gene059027:intron 40.0%
!! ATGATGAGACTAATGGGTTG+TGG - chr1.2:52092639-52092658 MS.gene059027:intron 40.0%
!! CCTTTTTCCCCTTTTGGAAA+TGG - chr1.2:52093842-52093861 MS.gene059027:CDS 40.0%
!! GGCTGTGTTGAACTTGTTAA+TGG - chr1.2:52093500-52093519 MS.gene059027:CDS 40.0%
!! TGTTTTGGATATTGGCACCT+TGG - chr1.2:52093421-52093440 MS.gene059027:intron 40.0%
!!! GGGAAGAAAGTTTTGGTTGT+TGG - chr1.2:52092830-52092849 MS.gene059027:intron 40.0%
!!! GTTCGGTTTTGAAACATCGT+TGG - chr1.2:52093716-52093735 MS.gene059027:CDS 40.0%
AGTCACCATTCCCTAATGGT+TGG - chr1.2:52093967-52093986 MS.gene059027:CDS 45.0%
ATCGTAGCAATGTCCCTTCT+TGG - chr1.2:52093564-52093583 MS.gene059027:CDS 45.0%
CAATGTCCCTTCTTGGCTTA+AGG - chr1.2:52093571-52093590 MS.gene059027:CDS 45.0%
CCAAAGTCACCATTCCCTAA+TGG - chr1.2:52093963-52093982 MS.gene059027:CDS 45.0%
CCATTAGGGAATGGTGACTT+TGG + chr1.2:52093966-52093985 None:intergenic 45.0%
CCATTCCCTAATGGTTGGAA+AGG - chr1.2:52093972-52093991 MS.gene059027:CDS 45.0%
CCTTTCCAACCATTAGGGAA+TGG + chr1.2:52093975-52093994 None:intergenic 45.0%
GAACACACTCGTTGAATCGT+GGG + chr1.2:52092607-52092626 None:intergenic 45.0%
GATGCAGTTGTTCTTGCTAC+TGG - chr1.2:52093539-52093558 MS.gene059027:CDS 45.0%
GCTGTTGGGTTTACAAAGAG+AGG - chr1.2:52094011-52094030 MS.gene059027:CDS 45.0%
GGTCACATAAAAGTTGTGCC+TGG - chr1.2:52093458-52093477 MS.gene059027:intron 45.0%
GGTCCTATAATAGTAGGAGC+AGG - chr1.2:52092338-52092357 MS.gene059027:CDS 45.0%
GGTCCTGCTCCTACTATTAT+AGG + chr1.2:52092344-52092363 None:intergenic 45.0%
GGTCTTAAAAGGCCATTGGA+GGG - chr1.2:52093362-52093381 MS.gene059027:intron 45.0%
GGTGCCAATATCCAAAACAG+GGG + chr1.2:52093420-52093439 None:intergenic 45.0%
TGAACACACTCGTTGAATCG+TGG + chr1.2:52092608-52092627 None:intergenic 45.0%
TTTACAAAGAGAGGGCTCTC+TGG - chr1.2:52094020-52094039 MS.gene059027:CDS 45.0%
! AAAGGGTAAGACCCCTGTTT+TGG - chr1.2:52093406-52093425 MS.gene059027:intron 45.0%
! GATTTCGAAGTGTTTCGCGT+AGG + chr1.2:52092582-52092601 None:intergenic 45.0%
! TGGTCTTAAAAGGCCATTGG+AGG - chr1.2:52093361-52093380 MS.gene059027:intron 45.0%
!! ACAACAAGGAGTACCCTTCA+TGG - chr1.2:52092394-52092413 MS.gene059027:CDS 45.0%
!!! AAAAAAATTATTTTATGTAT+TGG + chr1.2:52093789-52093808 None:intergenic 5.0%
ATAGTAGGAGCAGGACCATC+AGG - chr1.2:52092347-52092366 MS.gene059027:CDS 50.0%
AAGGCCATTGGAGGGTCCTT+TGG - chr1.2:52093370-52093389 MS.gene059027:intron 55.0%
AGCTCCAAAGGACCCTCCAA+TGG + chr1.2:52093377-52093396 None:intergenic 55.0%
AGGTCGCTTGTGGTTGCTAC+TGG - chr1.2:52092707-52092726 MS.gene059027:intron 55.0%
GCTGCTGTTGCTAGACCTGA+TGG + chr1.2:52092365-52092384 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 52092269 52094160 52092269 ID=MS.gene059027
chr1.2 mRNA 52092269 52094160 52092269 ID=MS.gene059027.t1;Parent=MS.gene059027
chr1.2 exon 52093498 52094160 52093498 ID=MS.gene059027.t1.exon1;Parent=MS.gene059027.t1
chr1.2 CDS 52093498 52094160 52093498 ID=cds.MS.gene059027.t1;Parent=MS.gene059027.t1
chr1.2 exon 52092837 52093208 52092837 ID=MS.gene059027.t1.exon2;Parent=MS.gene059027.t1
chr1.2 CDS 52092837 52093208 52092837 ID=cds.MS.gene059027.t1;Parent=MS.gene059027.t1
chr1.2 exon 52092269 52092496 52092269 ID=MS.gene059027.t1.exon3;Parent=MS.gene059027.t1
chr1.2 CDS 52092269 52092496 52092269 ID=cds.MS.gene059027.t1;Parent=MS.gene059027.t1
Gene Sequence

>MS.gene059027

ATGGAGAACTTGATTCGCCTAGTCGATAACGAAGAAGAAATAGTCTCACATCGTTGCATTTGGGTAAACGGTCCTATAATAGTAGGAGCAGGACCATCAGGTCTAGCAACAGCAGCATGTCTTAAACAACAAGGAGTACCCTTCATGGTGCTTGAAAGATCAAATTGCATAGCTTCACTGTGGCAAAAAAGAACATACGACAGATTAAAACTTCACCTCCCTAAAAAATTCTGTCAACTACCTAACTTTCCATTCCCACAAGAATTTCCTGAATATCCAACAAAGAAACAGTTCATAAACTATCTTGAATCCTACGCGAAACACTTCGAAATCAACCCACGATTCAACGAGTGTGTTCAATCTGCTAAATATGATGAGACTAATGGGTTGTGGAGAGTTGAGACTGTTTCCAATGGAAATGAAGTTGAGTATATTTGTAGGTCGCTTGTGGTTGCTACTGGTGAGAATGCAGAGTGTGTTGTGCCTGATATTGAAGGGTTGAGTAAATTCAAAGGTGAAGTTATTCATGCTTGTGATTATAAATCAGGTGAAGGTTTTAGAGGGAAGAAAGTTTTGGTTGTTGGTTGTGGTAATTCTGGAATGGAACTCTCACTTGATCTTTGCAATCATAATGCATCACCGTCTATTGTTCTTCGTAGCTCGGTAAGTACTTGAAATAAGTCAATTTAGTCTCTGAAATAAACGAAACTATAAAAAAATAATTTCTTGAAAATCTTTGACTACACAAGTTTTTTCGAGTCAGTCAACTTAGTTTCTAAAATTGCTCTGAAAGAACTAATGTGGTCGAAGATTTTTAAGCTTGGGGAATAATTATGATTTTGTTTATTTCGAGGACTAAATTTGTTGACAAATTTTTTTAAGAAAAATAATTTGAGGATGATTTTTTTTTTTTTTTGTACTAATTAAGGTTGTTGTGTTTTTGGTTGAACAGGTTCATGTTTTGCCTAGAGAAGTTTTTGGGAAATCAACATTTGGATTGGCAGTTATGATGATGAAATGGTTACCCGTTTGGATAGTTGACAAGATTCTTTTGATTTTGGCTTGGTTAGTGTTTGGTAATATAGAAAAGTATGGTCTTAAAAGGCCATTGGAGGGTCCTTTGGAGCTTAAGAACACAAAGGGTAAGACCCCTGTTTTGGATATTGGCACCTTGGAGAAAATTAGATCTGGTCACATAAAAGTTGTGCCTGGAATTAAGAGGTTTACTAATGGCTGTGTTGAACTTGTTAATGGTGAAAAGAGTCAAGTTGATGCAGTTGTTCTTGCTACTGGTTATCGTAGCAATGTCCCTTCTTGGCTTAAGGTGAGAAATTAAATTTTTATTTTTTTAATTTATTTTGTGTGTTTGTATAAATTTTTAAAAATTGAACCGAGCAGTTCAAAACAGTTGAACCATGATTCATAGGTCTTGCAAATTTGATGTTTGGTTCGGTTTTGAAACATCGTTGGTTTTGAAACCTTTTTTTGGGTCTTATCATATGTTTCTCTTTTTTCTTCCAATACATAAAATAATTTTTTTATGTTGTTTTGAAGTTTTAGAAGCTTCTTTTTACCTTTTTCCCCTTTTGGAAATGGAATTAGTATTTTACTATTTTTGTGTGGAGTTAATAACTAATCTTATTTTGGTTCATTTTTGTGTGTAGGAAGGTGAATTTTTCTCCAAGAATGGATTTCCAAAGTCACCATTCCCTAATGGTTGGAAAGGAACTGTTGGACTTTATGCTGTTGGGTTTACAAAGAGAGGGCTCTCTGGTTCTTCATATGATGCTAAACAAATTGCTCAAGATATTGCTCAAGTTTGGAAACAAGAGATTAAGCAAAAGAAACACTACACTAATGCTTGCAATAAACGTTGCATTTCCCAATTCTAA
Protein sequence

>MS.gene059027.t1

MENLIRLVDNEEEIVSHRCIWVNGPIIVGAGPSGLATAACLKQQGVPFMVLERSNCIASLWQKRTYDRLKLHLPKKFCQLPNFPFPQEFPEYPTKKQFINYLESYAKHFEINPRFNECVQSAKYDETNGLWRVETVSNGNEVEYICRSLVVATGENAECVVPDIEGLSKFKGEVIHACDYKSGEGFRGKKVLVVGCGNSGMELSLDLCNHNASPSIVLRSSVHVLPREVFGKSTFGLAVMMMKWLPVWIVDKILLILAWLVFGNIEKYGLKRPLEGPLELKNTKGKTPVLDIGTLEKIRSGHIKVVPGIKRFTNGCVELVNGEKSQVDAVVLATGYRSNVPSWLKEGEFFSKNGFPKSPFPNGWKGTVGLYAVGFTKRGLSGSSYDAKQIAQDIAQVWKQEIKQKKHYTNACNKRCISQF