AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64265

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64265.t1 MTR_1g046230 97.167 353 10 0 1 353 1 353 0.0 707
MS.gene64265.t1 MTR_7g099330 72.316 354 89 2 1 353 1 346 0.0 520
MS.gene64265.t1 MTR_1g069275 72.832 346 89 2 9 353 10 351 0.0 513
MS.gene64265.t1 MTR_1g008380 59.347 337 134 1 16 352 34 367 1.21e-148 426
MS.gene64265.t1 MTR_3g109520 59.104 335 135 1 19 353 34 366 6.02e-147 422
MS.gene64265.t1 MTR_6g086870 58.485 330 136 1 20 348 16 345 1.65e-146 420
MS.gene64265.t1 MTR_1g011630 56.047 339 143 1 15 353 7 339 2.69e-143 412
MS.gene64265.t1 MTR_7g099160 71.483 263 66 2 92 353 213 467 2.10e-127 376
MS.gene64265.t1 MTR_7g107710 46.933 326 164 5 25 348 7 325 5.64e-99 298
MS.gene64265.t1 MTR_7g107690 44.308 325 173 3 25 348 9 326 4.16e-98 296
MS.gene64265.t1 MTR_8g432640 44.073 329 176 4 25 352 7 328 1.07e-95 290
MS.gene64265.t1 MTR_5g033260 44.848 330 172 5 25 352 7 328 1.79e-95 289
MS.gene64265.t1 MTR_4g051642 42.988 328 175 4 25 349 8 326 3.74e-92 281
MS.gene64265.t1 MTR_3g088925 41.768 328 179 4 25 349 8 326 6.25e-90 276
MS.gene64265.t1 MTR_3g088945 41.463 328 180 4 25 349 8 326 4.60e-89 273
MS.gene64265.t1 MTR_3g088955 41.768 328 179 4 25 349 8 326 1.44e-88 272
MS.gene64265.t1 MTR_3g096030 38.764 178 83 3 25 202 7 158 4.33e-35 126
MS.gene64265.t1 MTR_2g450310 26.842 380 207 19 25 342 6 376 1.48e-15 78.2
MS.gene64265.t1 MTR_2g450510 24.934 377 218 15 25 342 6 376 8.52e-13 69.7
MS.gene64265.t1 MTR_2g044140 25.916 382 219 15 16 339 6 381 2.90e-12 68.2
MS.gene64265.t1 MTR_3g437580 22.917 240 142 6 26 232 15 244 1.23e-11 65.5
MS.gene64265.t1 MTR_2g020630 26.984 252 143 11 16 230 6 253 1.88e-11 65.5
MS.gene64265.t1 MTR_3g437580 22.917 240 142 6 26 232 15 244 2.31e-11 65.1
MS.gene64265.t1 MTR_2g043850 24.397 373 216 16 26 339 16 381 8.75e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64265.t1 AT1G04610 74.221 353 88 2 4 353 15 367 0.0 548
MS.gene64265.t1 AT2G33230 74.063 347 89 1 4 349 12 358 0.0 523
MS.gene64265.t1 AT4G28720 70.640 344 101 0 11 354 12 355 0.0 519
MS.gene64265.t1 AT5G43890 72.619 336 90 2 15 349 16 350 1.12e-177 500
MS.gene64265.t1 AT1G04180 69.477 344 100 3 11 353 12 351 3.90e-172 486
MS.gene64265.t1 AT4G13260 57.186 334 141 1 15 348 19 350 4.91e-145 417
MS.gene64265.t1 AT5G25620 59.821 336 129 2 16 349 24 355 2.50e-144 415
MS.gene64265.t1 AT5G25620 59.821 336 129 2 16 349 24 355 4.78e-144 415
MS.gene64265.t1 AT5G11320 58.507 335 131 2 19 353 12 338 4.98e-139 401
MS.gene64265.t1 AT5G11320 56.447 349 136 3 19 359 12 352 5.60e-138 397
MS.gene64265.t1 AT4G32540 51.929 337 154 2 19 354 16 345 2.72e-132 384
MS.gene64265.t1 AT4G32540 51.622 339 154 3 19 354 16 347 1.18e-130 380
MS.gene64265.t1 AT4G13260 57.823 294 122 1 15 308 19 310 3.00e-129 373
MS.gene64265.t1 AT1G21430 46.462 325 164 5 25 348 10 325 7.24e-98 296
MS.gene64265.t1 AT1G48910 42.424 330 180 4 25 352 6 327 2.48e-95 289
MS.gene64265.t1 AT4G13260 53.937 254 107 2 95 348 4 247 1.74e-91 276
MS.gene64265.t1 AT1G19250 25.401 374 213 16 25 339 14 380 7.68e-17 82.0
MS.gene64265.t1 AT5G45180 26.346 353 196 14 25 339 14 340 1.73e-16 80.9
MS.gene64265.t1 AT1G62620 23.673 245 145 7 26 237 15 250 9.85e-12 66.2
MS.gene64265.t1 AT1G62620 23.577 246 146 7 25 237 14 250 2.19e-11 65.5
MS.gene64265.t1 AT1G62560 23.404 235 139 7 25 230 14 236 3.43e-11 64.7
MS.gene64265.t1 AT1G63370 22.727 242 151 6 26 237 15 250 4.44e-11 63.9
MS.gene64265.t1 AT1G62560 23.404 235 139 7 25 230 14 236 4.48e-11 64.3

Find 78 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTCTGACTCGTGTGATTT+TGG 0.152127 1.3:+27905132 MS.gene64265:CDS
GTTGTTGGCTGTGGAAATTC+TGG 0.197319 1.3:+27904823 MS.gene64265:CDS
TTCGAAGGTTTGGCTGATTT+TGG 0.199341 1.3:+27904736 MS.gene64265:CDS
CCATTGATGGATTTGCATTA+TGG 0.206435 1.3:-27904873 None:intergenic
AGCACTGTTTCATTGAAATT+TGG 0.226962 1.3:-27904571 None:intergenic
TTTACAAGGAGAGGTCTTTC+TGG 0.245828 1.3:+27905668 MS.gene64265:intron
TGATGAAAAGGTTGCCAATT+TGG 0.246817 1.3:+27905089 MS.gene64265:CDS
GTGTTACCAAGGGAAATATT+TGG 0.264585 1.3:+27905037 MS.gene64265:CDS
GTGATCAGGGAAAGGAAAAT+TGG 0.273886 1.3:-27904476 None:intergenic
TCGAAAAGTATGGTCTTAAA+AGG 0.282601 1.3:+27905161 MS.gene64265:CDS
AGGTCTTTCTGGTGCTTCAT+TGG 0.310265 1.3:+27905679 MS.gene64265:CDS
GGTAGTTGACACAATTGTTT+AGG 0.340912 1.3:-27904454 None:intergenic
TTAGAGCTTAAGAACACTTC+AGG 0.349301 1.3:+27905199 MS.gene64265:CDS
ATCTTATCGACCATCCAAAT+TGG 0.356347 1.3:-27905103 None:intergenic
GGTCAAATTAAAGTTGTTCC+TGG 0.367198 1.3:+27905268 MS.gene64265:CDS
GAGCTTCTAACAACCATTGA+TGG 0.371002 1.3:-27904886 None:intergenic
ATGATGAAACCTTTGGTCTA+TGG 0.372822 1.3:+27904605 MS.gene64265:CDS
CAATGTACCATCTTGGCTTA+AGG 0.373912 1.3:+27905381 MS.gene64265:CDS
AGCAGTTGCAGCTTGTCTTA+AGG 0.375176 1.3:+27904339 MS.gene64265:CDS
TTGGGGAATGTCGAAAAGTA+TGG 0.384596 1.3:+27905151 MS.gene64265:CDS
GATTCTGTTGTTCTTGCTAC+TGG 0.392350 1.3:+27905349 MS.gene64265:CDS
TGAGCCGGAGTTCGAAGGTT+TGG 0.396120 1.3:+27904726 MS.gene64265:CDS
GGACGACGAAAGGAACATTT+TGG 0.398512 1.3:-27904363 None:intergenic
ATAGTTGGAGCTGGTCCATC+AGG 0.401112 1.3:+27904313 MS.gene64265:CDS
AAGACACCAGTATTAGATAT+TGG 0.401231 1.3:+27905223 MS.gene64265:CDS
GGGAAAGTTGAACTTGTTGA+TGG 0.406344 1.3:+27905310 MS.gene64265:CDS
GGTTGCTACCGGTGAAAATT+CGG 0.407888 1.3:+27904696 MS.gene64265:CDS
TGGTCTTAAAAGGCCATGTA+TGG 0.410967 1.3:+27905171 MS.gene64265:CDS
GGGTATTCAGGGAAGTGATC+AGG 0.411727 1.3:-27904490 None:intergenic
TATGGAGGATTAAGAGCAAT+AGG 0.415166 1.3:+27904623 MS.gene64265:CDS
GGACCAGCTCCAACTATAAC+AGG 0.417852 1.3:-27904307 None:intergenic
TTGAAGTTGAGTATATTTGT+AGG 0.421139 1.3:+27904665 MS.gene64265:CDS
CATGCTTGTGACTATAAATC+TGG 0.421528 1.3:+27904772 MS.gene64265:CDS
GCTAAGTATGATGAAACCTT+TGG 0.428804 1.3:+27904598 MS.gene64265:CDS
AGTGCTCCAATATCTAATAC+TGG 0.440252 1.3:-27905229 None:intergenic
GTGAATGGACCTGTTATAGT+TGG 0.443384 1.3:+27904298 MS.gene64265:CDS
ATTTGGAAAATCTACTTATG+AGG 0.443961 1.3:+27905054 MS.gene64265:CDS
AATGTTCTTGTTGTTGGCTG+TGG 0.445856 1.3:+27904814 MS.gene64265:CDS
TGATCAGGGAAAGGAAAATT+GGG 0.452224 1.3:-27904475 None:intergenic
CAATGCAGTTAGCTCTTTCG+AGG 0.466628 1.3:-27904384 None:intergenic
CTAACTGCATTGCTTCCCTT+TGG 0.470550 1.3:+27904395 MS.gene64265:CDS
GAAGTGTTCTTAAGCTCTAA+AGG 0.482235 1.3:-27905196 None:intergenic
ATAGAAGCAATGTACCATCT+TGG 0.483598 1.3:+27905374 MS.gene64265:CDS
TAGCTGTTATGTTGATGAAA+AGG 0.494702 1.3:+27905077 MS.gene64265:CDS
TAGAGCTTAAGAACACTTCA+GGG 0.496144 1.3:+27905200 MS.gene64265:CDS
CCATAATGCAAATCCATCAA+TGG 0.497074 1.3:+27904873 MS.gene64265:CDS
AACCGTTGCATATTAGTGAA+TGG 0.502495 1.3:+27904283 MS.gene64265:CDS
GGGTCGAATGTTTGAGACAT+TGG 0.515170 1.3:-27904244 None:intergenic
AGGTGGCTTGTGGTTGCTAC+CGG 0.524010 1.3:+27904685 MS.gene64265:CDS
GCACTTGAGAAGGTTAGATC+TGG 0.524284 1.3:+27905247 MS.gene64265:CDS
ATCAATGGTTGTTAGAAGCT+CGG 0.527251 1.3:+27904888 MS.gene64265:CDS
AAATCTGGTGTGAATCATAG+TGG 0.531964 1.3:+27904787 MS.gene64265:CDS
AGCTCTAAAGGTCCCATACA+TGG 0.538443 1.3:-27905184 None:intergenic
ATGGTCGATAAGATATTGCT+TGG 0.541229 1.3:+27905112 MS.gene64265:CDS
CGTAGGTTCGATTCTGCCAA+AGG 0.545565 1.3:-27904411 None:intergenic
TCAGCCAAACCTTCGAACTC+CGG 0.546694 1.3:-27904730 None:intergenic
AAACTAACCTTAAGCCAAGA+TGG 0.557267 1.3:-27905388 None:intergenic
GGACCTGTTATAGTTGGAGC+TGG 0.559689 1.3:+27904304 MS.gene64265:CDS
GGTATTCAGGGAAGTGATCA+GGG 0.569347 1.3:-27904489 None:intergenic
GCTCTTTCGAGGACGACGAA+AGG 0.572773 1.3:-27904373 None:intergenic
AAAGTTGAGCCGGAGTTCGA+AGG 0.573012 1.3:+27904721 MS.gene64265:CDS
ATGAAACCTTTGGTCTATGG+AGG 0.575369 1.3:+27904608 MS.gene64265:CDS
ATGAAGTTTAAGACGATCGT+AGG 0.577864 1.3:-27904428 None:intergenic
CTTAATCCTCCATAGACCAA+AGG 0.582115 1.3:-27904614 None:intergenic
AGATATTGGAGCACTTGAGA+AGG 0.585530 1.3:+27905237 MS.gene64265:CDS
GTATATTTGTAGGTGGCTTG+TGG 0.588469 1.3:+27904675 MS.gene64265:CDS
TCGAATGTTTGAGACATTGG+TGG 0.588628 1.3:-27904241 None:intergenic
GCTGCAACTGCTAAGCCTGA+TGG 0.591010 1.3:-27904328 None:intergenic
GGTCTTAAAAGGCCATGTAT+GGG 0.595116 1.3:+27905172 MS.gene64265:CDS
AATAGGTTCATGTGTTACCA+AGG 0.598175 1.3:+27905026 MS.gene64265:intron
GTAGGTTCGATTCTGCCAAA+GGG 0.600880 1.3:-27904410 None:intergenic
ATAGGTTCATGTGTTACCAA+GGG 0.601879 1.3:+27905027 MS.gene64265:intron
AAGTTGAGTATATTTGTAGG+TGG 0.602864 1.3:+27904668 MS.gene64265:CDS
GAAAAGGTTGCCAATTTGGA+TGG 0.608907 1.3:+27905093 MS.gene64265:CDS
AAATTCGGAGAAAGTTGAGC+CGG 0.610705 1.3:+27904711 MS.gene64265:CDS
GTCCATTCACTAATATGCAA+CGG 0.611348 1.3:-27904285 None:intergenic
TCAGGGAAGTGATCAGGGAA+AGG 0.611951 1.3:-27904484 None:intergenic
TGGCTGTGGAAATTCTGGCA+TGG 0.638016 1.3:+27904828 MS.gene64265:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCACAAAAAAAATAAAGA+TGG - chr1.3:27904999-27905018 None:intergenic 15.0%
!!! TTTTGTGATTTTTGATTAAT+AGG + chr1.3:27905009-27905028 MS.gene64265:intron 15.0%
!!! GAATTAAAAGATTTTCACAA+GGG + chr1.3:27905290-27905309 MS.gene64265:CDS 20.0%
!!! TTATGAACTGAAATTTTGTA+GGG - chr1.3:27904513-27904532 None:intergenic 20.0%
! ATTTGGAAAATCTACTTATG+AGG + chr1.3:27905054-27905073 MS.gene64265:CDS 25.0%
! TTCTATGTTATGTATTGAAC+AGG + chr1.3:27905559-27905578 MS.gene64265:intron 25.0%
! TTCTTTGTAATTATACGTGT+CGG + chr1.3:27905420-27905439 MS.gene64265:intron 25.0%
! TTGAAGTTGAGTATATTTGT+AGG + chr1.3:27904665-27904684 MS.gene64265:CDS 25.0%
!! AATGAATTCTTTTCCAAAGA+TGG + chr1.3:27905584-27905603 MS.gene64265:intron 25.0%
!! CTTATGAACTGAAATTTTGT+AGG - chr1.3:27904514-27904533 None:intergenic 25.0%
!! GGAATTAAAAGATTTTCACA+AGG + chr1.3:27905289-27905308 MS.gene64265:CDS 25.0%
!!! TGTGAAAATCTTTTAATTCC+AGG - chr1.3:27905289-27905308 None:intergenic 25.0%
AAGACACCAGTATTAGATAT+TGG + chr1.3:27905223-27905242 MS.gene64265:CDS 30.0%
AAGTTGAGTATATTTGTAGG+TGG + chr1.3:27904668-27904687 MS.gene64265:CDS 30.0%
AGCACTGTTTCATTGAAATT+TGG - chr1.3:27904574-27904593 None:intergenic 30.0%
GACACGTATAATTACAAAGA+AGG - chr1.3:27905421-27905440 None:intergenic 30.0%
TATATGCTGTTGGATTTACA+AGG + chr1.3:27905654-27905673 MS.gene64265:intron 30.0%
! GATTTTCCAAATATTTCCCT+TGG - chr1.3:27905046-27905065 None:intergenic 30.0%
! GGAAAAAATGTTCTTGTTGT+TGG + chr1.3:27904808-27904827 MS.gene64265:CDS 30.0%
! TAGCTGTTATGTTGATGAAA+AGG + chr1.3:27905077-27905096 MS.gene64265:CDS 30.0%
! TCGAAAAGTATGGTCTTAAA+AGG + chr1.3:27905161-27905180 MS.gene64265:CDS 30.0%
!! GAAATTTTGTAGGGTATTCA+GGG - chr1.3:27904504-27904523 None:intergenic 30.0%
!! TGAAATTTTGTAGGGTATTC+AGG - chr1.3:27904505-27904524 None:intergenic 30.0%
!!! AGTTTTTAGCATATGATTCG+AGG - chr1.3:27904540-27904559 None:intergenic 30.0%
AAACTAACCTTAAGCCAAGA+TGG - chr1.3:27905391-27905410 None:intergenic 35.0%
AAATCTGGTGTGAATCATAG+TGG + chr1.3:27904787-27904806 MS.gene64265:CDS 35.0%
AACCGTTGCATATTAGTGAA+TGG + chr1.3:27904283-27904302 MS.gene64265:CDS 35.0%
AAGATCCATTTCCAAATGGA+TGG + chr1.3:27905615-27905634 MS.gene64265:intron 35.0%
AATAGGTTCATGTGTTACCA+AGG + chr1.3:27905026-27905045 MS.gene64265:intron 35.0%
AGTGCTCCAATATCTAATAC+TGG - chr1.3:27905232-27905251 None:intergenic 35.0%
ATAGAAGCAATGTACCATCT+TGG + chr1.3:27905374-27905393 MS.gene64265:CDS 35.0%
ATAGGTTCATGTGTTACCAA+GGG + chr1.3:27905027-27905046 MS.gene64265:intron 35.0%
ATCAATGGTTGTTAGAAGCT+CGG + chr1.3:27904888-27904907 MS.gene64265:CDS 35.0%
ATCTTATCGACCATCCAAAT+TGG - chr1.3:27905106-27905125 None:intergenic 35.0%
ATGATGAAACCTTTGGTCTA+TGG + chr1.3:27904605-27904624 MS.gene64265:CDS 35.0%
ATGGTCGATAAGATATTGCT+TGG + chr1.3:27905112-27905131 MS.gene64265:CDS 35.0%
CATGCTTGTGACTATAAATC+TGG + chr1.3:27904772-27904791 MS.gene64265:CDS 35.0%
CCAAAAGATCCATTTCCAAA+TGG + chr1.3:27905611-27905630 MS.gene64265:intron 35.0%
CCATAATGCAAATCCATCAA+TGG + chr1.3:27904873-27904892 MS.gene64265:CDS 35.0%
GCTAAGTATGATGAAACCTT+TGG + chr1.3:27904598-27904617 MS.gene64265:CDS 35.0%
GGTAGTTGACACAATTGTTT+AGG - chr1.3:27904457-27904476 None:intergenic 35.0%
GGTCAAATTAAAGTTGTTCC+TGG + chr1.3:27905268-27905287 MS.gene64265:CDS 35.0%
GTCCATTCACTAATATGCAA+CGG - chr1.3:27904288-27904307 None:intergenic 35.0%
GTGTTACCAAGGGAAATATT+TGG + chr1.3:27905037-27905056 MS.gene64265:CDS 35.0%
TAGAGCTTAAGAACACTTCA+GGG + chr1.3:27905200-27905219 MS.gene64265:CDS 35.0%
TATGGAGGATTAAGAGCAAT+AGG + chr1.3:27904623-27904642 MS.gene64265:CDS 35.0%
TGATCAGGGAAAGGAAAATT+GGG - chr1.3:27904478-27904497 None:intergenic 35.0%
TGATGAAAAGGTTGCCAATT+TGG + chr1.3:27905089-27905108 MS.gene64265:CDS 35.0%
TTAGAGCTTAAGAACACTTC+AGG + chr1.3:27905199-27905218 MS.gene64265:CDS 35.0%
! AACTTTCTCCGAATTTTCAC+CGG - chr1.3:27904707-27904726 None:intergenic 35.0%
! ATCTTTTGGCATTCCATCTT+TGG - chr1.3:27905600-27905619 None:intergenic 35.0%
! ATGAAGTTTAAGACGATCGT+AGG - chr1.3:27904431-27904450 None:intergenic 35.0%
! CCATTTGGAAATGGATCTTT+TGG - chr1.3:27905614-27905633 None:intergenic 35.0%
! GAAGTGTTCTTAAGCTCTAA+AGG - chr1.3:27905199-27905218 None:intergenic 35.0%
!! ACGGTTTTTGAGAATGTCTT+CGG - chr1.3:27904269-27904288 None:intergenic 35.0%
!! CCATTGATGGATTTGCATTA+TGG - chr1.3:27904876-27904895 None:intergenic 35.0%
AAATTCGGAGAAAGTTGAGC+CGG + chr1.3:27904711-27904730 MS.gene64265:CDS 40.0%
AATGGATGGAAAGGAAATGC+TGG + chr1.3:27905629-27905648 MS.gene64265:intron 40.0%
ATGAAACCTTTGGTCTATGG+AGG + chr1.3:27904608-27904627 MS.gene64265:CDS 40.0%
CAATGTACCATCTTGGCTTA+AGG + chr1.3:27905381-27905400 MS.gene64265:CDS 40.0%
CCATTTCCAAATGGATGGAA+AGG + chr1.3:27905620-27905639 MS.gene64265:intron 40.0%
CCTTTCCATCCATTTGGAAA+TGG - chr1.3:27905623-27905642 None:intergenic 40.0%
CTTAATCCTCCATAGACCAA+AGG - chr1.3:27904617-27904636 None:intergenic 40.0%
GAAAAGGTTGCCAATTTGGA+TGG + chr1.3:27905093-27905112 MS.gene64265:CDS 40.0%
GAGCTTCTAACAACCATTGA+TGG - chr1.3:27904889-27904908 None:intergenic 40.0%
GATTCTGTTGTTCTTGCTAC+TGG + chr1.3:27905349-27905368 MS.gene64265:CDS 40.0%
GCATTTCCTTTCCATCCATT+TGG - chr1.3:27905629-27905648 None:intergenic 40.0%
GGTCTTAAAAGGCCATGTAT+GGG + chr1.3:27905172-27905191 MS.gene64265:CDS 40.0%
GTATATTTGTAGGTGGCTTG+TGG + chr1.3:27904675-27904694 MS.gene64265:CDS 40.0%
GTGAATGGACCTGTTATAGT+TGG + chr1.3:27904298-27904317 MS.gene64265:CDS 40.0%
GTGATCAGGGAAAGGAAAAT+TGG - chr1.3:27904479-27904498 None:intergenic 40.0%
TATGAAGCACAGACACTCTT+CGG - chr1.3:27905498-27905517 None:intergenic 40.0%
TCTTCGGATTAAATGTGTCC+TGG - chr1.3:27905482-27905501 None:intergenic 40.0%
TTGGGGAATGTCGAAAAGTA+TGG + chr1.3:27905151-27905170 MS.gene64265:CDS 40.0%
TTTACAAGGAGAGGTCTTTC+TGG + chr1.3:27905668-27905687 MS.gene64265:intron 40.0%
! AATGCTGGTCTATATGCTGT+TGG + chr1.3:27905644-27905663 MS.gene64265:intron 40.0%
! AGATATTGGAGCACTTGAGA+AGG + chr1.3:27905237-27905256 MS.gene64265:CDS 40.0%
! TCGAATGTTTGAGACATTGG+TGG - chr1.3:27904244-27904263 None:intergenic 40.0%
! TGGTCTTAAAAGGCCATGTA+TGG + chr1.3:27905171-27905190 MS.gene64265:CDS 40.0%
!! AATGTTCTTGTTGTTGGCTG+TGG + chr1.3:27904814-27904833 MS.gene64265:CDS 40.0%
!! CGGTTTTTGAGAATGTCTTC+GGG - chr1.3:27904268-27904287 None:intergenic 40.0%
!! GGGAAAGTTGAACTTGTTGA+TGG + chr1.3:27905310-27905329 MS.gene64265:CDS 40.0%
!! GGTTTTTGAGAATGTCTTCG+GGG - chr1.3:27904267-27904286 None:intergenic 40.0%
!! TTCGAAGGTTTGGCTGATTT+TGG + chr1.3:27904736-27904755 MS.gene64265:CDS 40.0%
AGCAGTTGCAGCTTGTCTTA+AGG + chr1.3:27904339-27904358 MS.gene64265:CDS 45.0%
CTAACTGCATTGCTTCCCTT+TGG + chr1.3:27904395-27904414 MS.gene64265:CDS 45.0%
GCACTTGAGAAGGTTAGATC+TGG + chr1.3:27905247-27905266 MS.gene64265:CDS 45.0%
GCTGTTGGATTTACAAGGAG+AGG + chr1.3:27905659-27905678 MS.gene64265:intron 45.0%
GGTATTCAGGGAAGTGATCA+GGG - chr1.3:27904492-27904511 None:intergenic 45.0%
GGTTGCTACCGGTGAAAATT+CGG + chr1.3:27904696-27904715 MS.gene64265:CDS 45.0%
GTAGGTTCGATTCTGCCAAA+GGG - chr1.3:27904413-27904432 None:intergenic 45.0%
! AGGTCTTTCTGGTGCTTCAT+TGG + chr1.3:27905679-27905698 MS.gene64265:CDS 45.0%
! CAATGCAGTTAGCTCTTTCG+AGG - chr1.3:27904387-27904406 None:intergenic 45.0%
! GGACGACGAAAGGAACATTT+TGG - chr1.3:27904366-27904385 None:intergenic 45.0%
! GGGTCGAATGTTTGAGACAT+TGG - chr1.3:27904247-27904266 None:intergenic 45.0%
! GTTGTTGGCTGTGGAAATTC+TGG + chr1.3:27904823-27904842 MS.gene64265:CDS 45.0%
!! AGCTCTAAAGGTCCCATACA+TGG - chr1.3:27905187-27905206 None:intergenic 45.0%
!! GAAGGTTTGGCTGATTTTGG+TGG + chr1.3:27904739-27904758 MS.gene64265:CDS 45.0%
!! GGTCTGACTCGTGTGATTTT+GGG + chr1.3:27905133-27905152 MS.gene64265:CDS 45.0%
!! GTCTGACTCGTGTGATTTTG+GGG + chr1.3:27905134-27905153 MS.gene64265:CDS 45.0%
!! TATACGTGTCGGTGTTGTGT+CGG + chr1.3:27905431-27905450 MS.gene64265:intron 45.0%
!! TGGTCTGACTCGTGTGATTT+TGG + chr1.3:27905132-27905151 MS.gene64265:CDS 45.0%
AAAGTTGAGCCGGAGTTCGA+AGG + chr1.3:27904721-27904740 MS.gene64265:CDS 50.0%
ATAGTTGGAGCTGGTCCATC+AGG + chr1.3:27904313-27904332 MS.gene64265:CDS 50.0%
CGTAGGTTCGATTCTGCCAA+AGG - chr1.3:27904414-27904433 None:intergenic 50.0%
GGACCAGCTCCAACTATAAC+AGG - chr1.3:27904310-27904329 None:intergenic 50.0%
GGACCTGTTATAGTTGGAGC+TGG + chr1.3:27904304-27904323 MS.gene64265:CDS 50.0%
GGGTATTCAGGGAAGTGATC+AGG - chr1.3:27904493-27904512 None:intergenic 50.0%
TCAGCCAAACCTTCGAACTC+CGG - chr1.3:27904733-27904752 None:intergenic 50.0%
TCAGGGAAGTGATCAGGGAA+AGG - chr1.3:27904487-27904506 None:intergenic 50.0%
TGGCTGTGGAAATTCTGGCA+TGG + chr1.3:27904828-27904847 MS.gene64265:CDS 50.0%
CACGACACATGTCTGACACC+AGG + chr1.3:27905461-27905480 MS.gene64265:intron 55.0%
GCTCTTTCGAGGACGACGAA+AGG - chr1.3:27904376-27904395 None:intergenic 55.0%
GCTGCAACTGCTAAGCCTGA+TGG - chr1.3:27904331-27904350 None:intergenic 55.0%
TGAGCCGGAGTTCGAAGGTT+TGG + chr1.3:27904726-27904745 MS.gene64265:CDS 55.0%
! AGGTGGCTTGTGGTTGCTAC+CGG + chr1.3:27904685-27904704 MS.gene64265:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 27904238 27905711 27904238 ID=MS.gene64265
chr1.3 mRNA 27904238 27905711 27904238 ID=MS.gene64265.t1;Parent=MS.gene64265
chr1.3 exon 27904238 27904909 27904238 ID=MS.gene64265.t1.exon1;Parent=MS.gene64265.t1
chr1.3 CDS 27904238 27904909 27904238 ID=cds.MS.gene64265.t1;Parent=MS.gene64265.t1
chr1.3 exon 27905031 27905402 27905031 ID=MS.gene64265.t1.exon2;Parent=MS.gene64265.t1
chr1.3 CDS 27905031 27905402 27905031 ID=cds.MS.gene64265.t1;Parent=MS.gene64265.t1
chr1.3 exon 27905676 27905711 27905676 ID=MS.gene64265.t1.exon3;Parent=MS.gene64265.t1
chr1.3 CDS 27905676 27905711 27905676 ID=cds.MS.gene64265.t1;Parent=MS.gene64265.t1
Gene Sequence

>MS.gene64265

ATGCCACCAATGTCTCAAACATTCGACCCCGAAGACATTCTCAAAAACCGTTGCATATTAGTGAATGGACCTGTTATAGTTGGAGCTGGTCCATCAGGCTTAGCAGTTGCAGCTTGTCTTAAGGACCAAAATGTTCCTTTCGTCGTCCTCGAAAGAGCTAACTGCATTGCTTCCCTTTGGCAGAATCGAACCTACGATCGTCTTAAACTTCATCTTCCTAAACAATTGTGTCAACTACCCAATTTTCCTTTCCCTGATCACTTCCCTGAATACCCTACAAAATTTCAGTTCATAAGTTACCTCGAATCATATGCTAAAAACTTTAACATAACACCAAATTTCAATGAAACAGTGCTATCTGCTAAGTATGATGAAACCTTTGGTCTATGGAGGATTAAGAGCAATAGGATAAAAAGTGATAAAGTTATTGAAGTTGAGTATATTTGTAGGTGGCTTGTGGTTGCTACCGGTGAAAATTCGGAGAAAGTTGAGCCGGAGTTCGAAGGTTTGGCTGATTTTGGTGGAAATGTTATGCATGCTTGTGACTATAAATCTGGTGTGAATCATAGTGGAAAAAATGTTCTTGTTGTTGGCTGTGGAAATTCTGGCATGGAAGTTTCTCTTGATCTTTGTAACCATAATGCAAATCCATCAATGGTTGTTAGAAGCTCGGTGAGTACTTAATCGAAAATTAATTACTTCAATTAATTATGTTAATCTTGATTCGTAGTTTGCATTTTTATGTGTATAGATTGTGACCATCTTTATTTTTTTTGTGATTTTTGATTAATAGGTTCATGTGTTACCAAGGGAAATATTTGGAAAATCTACTTATGAGGTAGCTGTTATGTTGATGAAAAGGTTGCCAATTTGGATGGTCGATAAGATATTGCTTGGTCTGACTCGTGTGATTTTGGGGAATGTCGAAAAGTATGGTCTTAAAAGGCCATGTATGGGACCTTTAGAGCTTAAGAACACTTCAGGGAAGACACCAGTATTAGATATTGGAGCACTTGAGAAGGTTAGATCTGGTCAAATTAAAGTTGTTCCTGGAATTAAAAGATTTTCACAAGGGAAAGTTGAACTTGTTGATGGAAAAGTTCTTGATATTGATTCTGTTGTTCTTGCTACTGGTTATAGAAGCAATGTACCATCTTGGCTTAAGGTTAGTTTGTTTAGTCCTTCTTTGTAATTATACGTGTCGGTGTTGTGTCGGTATCAGACACGACACATGTCTGACACCAGGACACATTTAATCCGAAGAGTGTCTGTGCTTCATAGTACTTACTAGTTCATTTTGTTTAAGTTGATTATTTAATTTTTCTATGTTATGTATTGAACAGGAGAATGAATTCTTTTCCAAAGATGGAATGCCAAAAGATCCATTTCCAAATGGATGGAAAGGAAATGCTGGTCTATATGCTGTTGGATTTACAAGGAGAGGTCTTTCTGGTGCTTCATTGGATGCAATTAG
Protein sequence

>MS.gene64265.t1

MPPMSQTFDPEDILKNRCILVNGPVIVGAGPSGLAVAACLKDQNVPFVVLERANCIASLWQNRTYDRLKLHLPKQLCQLPNFPFPDHFPEYPTKFQFISYLESYAKNFNITPNFNETVLSAKYDETFGLWRIKSNRIKSDKVIEVEYICRWLVVATGENSEKVEPEFEGLADFGGNVMHACDYKSGVNHSGKNVLVVGCGNSGMEVSLDLCNHNANPSMVVRSSVHVLPREIFGKSTYEVAVMLMKRLPIWMVDKILLGLTRVILGNVEKYGLKRPCMGPLELKNTSGKTPVLDIGALEKVRSGQIKVVPGIKRFSQGKVELVDGKVLDIDSVVLATGYRSNVPSWLKERSFWCFIGCN