AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065136


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065136.t1 MTR_1g110760 95.092 163 5 1 1 163 1 160 3.83e-106 300
MS.gene065136.t1 MTR_6g018430 46.584 161 66 6 4 163 27 168 1.05e-34 119
MS.gene065136.t1 MTR_8g104210 46.012 163 67 8 7 163 16 163 5.67e-29 104
MS.gene065136.t1 MTR_8g104210 46.012 163 67 8 7 163 25 172 9.32e-29 104
MS.gene065136.t1 MTR_6g084470 45.122 164 68 7 5 163 21 167 1.64e-25 96.3
MS.gene065136.t1 MTR_1g051170 38.931 131 60 4 26 149 31 148 5.63e-13 63.2
MS.gene065136.t1 MTR_4g087400 38.732 142 58 5 9 149 22 135 1.89e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065136.t1 AT1G68490 59.756 164 53 6 4 163 29 183 3.89e-49 157
MS.gene065136.t1 AT1G13390 52.469 162 65 5 4 163 25 176 4.72e-42 138
MS.gene065136.t1 AT1G13390 52.469 162 65 5 4 163 25 176 4.72e-42 138
MS.gene065136.t1 AT5G16110 50.909 165 67 7 4 163 89 244 1.87e-35 124
MS.gene065136.t1 AT3G02555 46.296 162 56 5 5 163 29 162 2.09e-27 100
MS.gene065136.t1 AT3G02555 46.296 162 56 5 5 163 29 162 2.09e-27 100

Find 65 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCGGATTGTTACCAAAAT+TGG 0.118190 1.2:-82058983 None:intergenic
GGATGTGTTCGGGCCAATTT+TGG 0.234336 1.2:+82058970 MS.gene065136:CDS
AGTCCGACACGGCGGGGCTT+AGG 0.339729 1.2:-82058255 None:intergenic
AGTAGCTAACCCACTAATTC+AGG 0.341828 1.2:+82058837 MS.gene065136:CDS
TAATTCAGGATGCTCGATTC+GGG 0.363565 1.2:+82058851 MS.gene065136:CDS
CCTCCCTTCCTTGCAGAAGA+TGG 0.393816 1.2:-82058949 None:intergenic
CTAATTCAGGATGCTCGATT+CGG 0.401608 1.2:+82058850 MS.gene065136:CDS
AGAAGGTGGCAGACCGGATG+GGG 0.404001 1.2:-82058923 None:intergenic
GAGCAGGGATGCTGCAATTA+CGG 0.410027 1.2:-82059044 None:intergenic
CGCCCCTCTCACCGTCGTCA+TGG 0.419708 1.2:+82058887 MS.gene065136:CDS
GTCCGACACGGCGGGGCTTA+GGG 0.420772 1.2:-82058254 None:intergenic
CAAGGAAGGGAGGATGTGTT+CGG 0.421741 1.2:+82058959 MS.gene065136:CDS
GATCCTTCCGGTGGCATCTC+AGG 0.425952 1.2:+82058310 MS.gene065136:CDS
CTCCCTTCCTTGCAGAAGAT+GGG 0.432493 1.2:-82058948 None:intergenic
AAGGATTCGATTGCCTTGAC+AGG 0.436802 1.2:+82059016 MS.gene065136:CDS
TAATTGCAGCATCCCTGCTC+TGG 0.448656 1.2:+82059047 MS.gene065136:CDS
GCCCCTCTCACCGTCGTCAT+GGG 0.457309 1.2:+82058888 MS.gene065136:CDS
GGAGAAGGTGGCAGACCGGA+TGG 0.458776 1.2:-82058925 None:intergenic
CTCATCTGCGTCAAATCCCT+TGG 0.463633 1.2:+82058459 MS.gene065136:CDS
GGTGGCAGACCGGATGGGGC+AGG 0.466438 1.2:-82058919 None:intergenic
TGCCCTAAGCCCCGCCGTGT+CGG 0.473384 1.2:+82058252 MS.gene065136:CDS
CTGTTGTTCTAAGCCAGAGC+AGG 0.481278 1.2:-82059060 None:intergenic
GAGAAGGTGGCAGACCGGAT+GGG 0.484866 1.2:-82058924 None:intergenic
ACTACCCGAGCAGATCCTTC+CGG 0.494170 1.2:+82058298 MS.gene065136:CDS
GTGGTGGTTCCTGCCCCATC+CGG 0.495940 1.2:+82058910 MS.gene065136:CDS
TTGAATCACACGGTTCAACT+TGG 0.496164 1.2:-82058435 None:intergenic
GTTGAGGAGTCCGACACGGC+GGG 0.496709 1.2:-82058262 None:intergenic
CTCACCGTCGTCATGGGTGG+TGG 0.513742 1.2:+82058894 MS.gene065136:CDS
TCTGCTCGGGTAGTCGTTGA+CGG 0.526759 1.2:-82058289 None:intergenic
CCACCCATGACGACGGTGAG+AGG 0.533000 1.2:-82058891 None:intergenic
AATCCGGCGGTGAGAGTCGA+AGG 0.545936 1.2:+82058997 MS.gene065136:CDS
TCCCTTCCTTGCAGAAGATG+GGG 0.546203 1.2:-82058947 None:intergenic
AATCGAGCATCCTGAATTAG+TGG 0.550154 1.2:-82058847 None:intergenic
AGTCGTTGACGGCGAAGTTG+AGG 0.554697 1.2:-82058278 None:intergenic
CACCCATGACGACGGTGAGA+GGG 0.562391 1.2:-82058890 None:intergenic
AAGGAAGGGAGGATGTGTTC+GGG 0.568345 1.2:+82058960 MS.gene065136:CDS
GCCACCGGAAGGATCTGCTC+GGG 0.575084 1.2:-82058302 None:intergenic
TTGGTGAGAATTGTGTCCAA+GGG 0.586012 1.2:-82058475 None:intergenic
CCTCTCACCGTCGTCATGGG+TGG 0.588491 1.2:+82058891 MS.gene065136:CDS
CTTGGTGAGAATTGTGTCCA+AGG 0.590478 1.2:-82058476 None:intergenic
GCAGGATGATGATTGTGATA+CGG 0.592153 1.2:+82058750 MS.gene065136:intron
AGTGGGTTAGCTACTCTGCT+CGG 0.596257 1.2:-82058829 None:intergenic
TGCCACCGGAAGGATCTGCT+CGG 0.596625 1.2:-82058303 None:intergenic
AGGATTCGATTGCCTTGACA+GGG 0.597575 1.2:+82059017 MS.gene065136:CDS
CCATCTTCTGCAAGGAAGGG+AGG 0.605126 1.2:+82058949 MS.gene065136:CDS
CCCTTCCTTGCAGAAGATGG+GGG 0.610451 1.2:-82058946 None:intergenic
ACCCATGACGACGGTGAGAG+GGG 0.613698 1.2:-82058889 None:intergenic
AATCCTTCGACTCTCACCGC+CGG 0.616188 1.2:-82059000 None:intergenic
TTTCGACGAAGGAAGATGAA+CGG 0.620932 1.2:+82058213 None:intergenic
AATTACGGCTGTCCCTGTCA+AGG 0.629306 1.2:-82059029 None:intergenic
ATCGAGCATCCTGAATTAGT+GGG 0.630666 1.2:-82058846 None:intergenic
GGCGGCGACGAGGCTACTGG+AGG 0.632101 1.2:-82058784 None:intergenic
AAATTCACCTGAGATGCCAC+CGG 0.634810 1.2:-82058317 None:intergenic
TCACCTGAGATGCCACCGGA+AGG 0.642394 1.2:-82058313 None:intergenic
CGAAGTTGAGGAGTCCGACA+CGG 0.647237 1.2:-82058266 None:intergenic
GGAACCACCACCCATGACGA+CGG 0.659927 1.2:-82058898 None:intergenic
GCAGATGAGTTTGAATCACA+CGG 0.662013 1.2:-82058445 None:intergenic
TGTTGTTCTAAGCCAGAGCA+GGG 0.670625 1.2:-82059059 None:intergenic
ACCCGAGCAGATCCTTCCGG+TGG 0.672658 1.2:+82058301 MS.gene065136:CDS
AATTCAGGATGCTCGATTCG+GGG 0.678593 1.2:+82058852 MS.gene065136:CDS
AGTTGAGGAGTCCGACACGG+CGG 0.682882 1.2:-82058263 None:intergenic
TTGAGGAGTCCGACACGGCG+GGG 0.686362 1.2:-82058261 None:intergenic
GCGGGGCTTAGGGCAAACGA+CGG 0.692402 1.2:-82058244 None:intergenic
CTTGGACACAATTCTCACCA+AGG 0.706031 1.2:+82058477 MS.gene065136:CDS
GGGTTAGCTACTCTGCTCGG+CGG 0.725325 1.2:-82058826 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCATATAAAATAATAG+AGG - chr1.2:82058512-82058531 None:intergenic 10.0%
!!! CTATTATTTTATATGAATTT+AGG + chr1.2:82058512-82058531 MS.gene065136:intron 10.0%
!!! AATTGATCTATTTTTAACAT+TGG + chr1.2:82058590-82058609 MS.gene065136:intron 15.0%
!!! ATATGAATTTAGGTTAAAAT+TGG + chr1.2:82058522-82058541 MS.gene065136:intron 15.0%
!!! TTTTCTCTTTTAATTTGCTT+TGG + chr1.2:82058362-82058381 MS.gene065136:intron 20.0%
! AATAGAGGAATTATTTACCT+TGG - chr1.2:82058497-82058516 None:intergenic 25.0%
ACACAAAGCAATTCAACAAA+AGG - chr1.2:82058686-82058705 None:intergenic 30.0%
AATCGAGCATCCTGAATTAG+TGG - chr1.2:82058850-82058869 None:intergenic 40.0%
AGTAGCTAACCCACTAATTC+AGG + chr1.2:82058837-82058856 MS.gene065136:CDS 40.0%
ATCGAGCATCCTGAATTAGT+GGG - chr1.2:82058849-82058868 None:intergenic 40.0%
CTAATTCAGGATGCTCGATT+CGG + chr1.2:82058850-82058869 MS.gene065136:CDS 40.0%
GCAGATGAGTTTGAATCACA+CGG - chr1.2:82058448-82058467 None:intergenic 40.0%
GCAGGATGATGATTGTGATA+CGG + chr1.2:82058750-82058769 MS.gene065136:intron 40.0%
TAATTCAGGATGCTCGATTC+GGG + chr1.2:82058851-82058870 MS.gene065136:CDS 40.0%
TTGAATCACACGGTTCAACT+TGG - chr1.2:82058438-82058457 None:intergenic 40.0%
TTGGTGAGAATTGTGTCCAA+GGG - chr1.2:82058478-82058497 None:intergenic 40.0%
!! CAATTTTGGTAACAATCCGG+CGG + chr1.2:82058984-82059003 MS.gene065136:CDS 40.0%
!! GGCCAATTTTGGTAACAATC+CGG + chr1.2:82058981-82059000 MS.gene065136:CDS 40.0%
!! GTTGTGCTTTTGTGATTTGC+AGG + chr1.2:82058732-82058751 MS.gene065136:intron 40.0%
AAATTCACCTGAGATGCCAC+CGG - chr1.2:82058320-82058339 None:intergenic 45.0%
AAGGATTCGATTGCCTTGAC+AGG + chr1.2:82059016-82059035 MS.gene065136:CDS 45.0%
AGGATTCGATTGCCTTGACA+GGG + chr1.2:82059017-82059036 MS.gene065136:CDS 45.0%
CGCCGGATTGTTACCAAAAT+TGG - chr1.2:82058986-82059005 None:intergenic 45.0%
CTTGGACACAATTCTCACCA+AGG + chr1.2:82058477-82058496 MS.gene065136:CDS 45.0%
CTTGGTGAGAATTGTGTCCA+AGG - chr1.2:82058479-82058498 None:intergenic 45.0%
! AATTCAGGATGCTCGATTCG+GGG + chr1.2:82058852-82058871 MS.gene065136:CDS 45.0%
AAGGAAGGGAGGATGTGTTC+GGG + chr1.2:82058960-82058979 MS.gene065136:CDS 50.0%
AATTACGGCTGTCCCTGTCA+AGG - chr1.2:82059032-82059051 None:intergenic 50.0%
CAAGGAAGGGAGGATGTGTT+CGG + chr1.2:82058959-82058978 MS.gene065136:CDS 50.0%
CGGAGACCCACAGAAAAATG+GGG - chr1.2:82058809-82058828 None:intergenic 50.0%
CTCATCTGCGTCAAATCCCT+TGG + chr1.2:82058459-82058478 MS.gene065136:CDS 50.0%
CTCCCTTCCTTGCAGAAGAT+GGG - chr1.2:82058951-82058970 None:intergenic 50.0%
GAGCAGGGATGCTGCAATTA+CGG - chr1.2:82059047-82059066 None:intergenic 50.0%
GCGGAGACCCACAGAAAAAT+GGG - chr1.2:82058810-82058829 None:intergenic 50.0%
GGAGACCCACAGAAAAATGG+GGG - chr1.2:82058808-82058827 None:intergenic 50.0%
TAATTGCAGCATCCCTGCTC+TGG + chr1.2:82059047-82059066 MS.gene065136:CDS 50.0%
TCCCTTCCTTGCAGAAGATG+GGG - chr1.2:82058950-82058969 None:intergenic 50.0%
! GGATGTGTTCGGGCCAATTT+TGG + chr1.2:82058970-82058989 MS.gene065136:CDS 50.0%
!! AGTGGGTTAGCTACTCTGCT+CGG - chr1.2:82058832-82058851 None:intergenic 50.0%
AATCCTTCGACTCTCACCGC+CGG - chr1.2:82059003-82059022 None:intergenic 55.0%
ACTACCCGAGCAGATCCTTC+CGG + chr1.2:82058298-82058317 MS.gene065136:CDS 55.0%
AGTCGTTGACGGCGAAGTTG+AGG - chr1.2:82058281-82058300 None:intergenic 55.0%
CCATCTTCTGCAAGGAAGGG+AGG + chr1.2:82058949-82058968 MS.gene065136:CDS 55.0%
CCCCCATCTTCTGCAAGGAA+GGG + chr1.2:82058946-82058965 MS.gene065136:CDS 55.0%
CCCTTCCTTGCAGAAGATGG+GGG - chr1.2:82058949-82058968 None:intergenic 55.0%
CCTCCCTTCCTTGCAGAAGA+TGG - chr1.2:82058952-82058971 None:intergenic 55.0%
CGAAGTTGAGGAGTCCGACA+CGG - chr1.2:82058269-82058288 None:intergenic 55.0%
CTTCTCCCCCATCTTCTGCA+AGG + chr1.2:82058941-82058960 MS.gene065136:CDS 55.0%
CTTGCAGAAGATGGGGGAGA+AGG - chr1.2:82058943-82058962 None:intergenic 55.0%
GACCCACAGAAAAATGGGGG+CGG - chr1.2:82058805-82058824 None:intergenic 55.0%
GGCGGAGACCCACAGAAAAA+TGG - chr1.2:82058811-82058830 None:intergenic 55.0%
TCCCCCATCTTCTGCAAGGA+AGG + chr1.2:82058945-82058964 MS.gene065136:CDS 55.0%
TCTGCTCGGGTAGTCGTTGA+CGG - chr1.2:82058292-82058311 None:intergenic 55.0%
AATCCGGCGGTGAGAGTCGA+AGG + chr1.2:82058997-82059016 MS.gene065136:CDS 60.0%
ACCCATGACGACGGTGAGAG+GGG - chr1.2:82058892-82058911 None:intergenic 60.0%
AGAAGGTGGCAGACCGGATG+GGG - chr1.2:82058926-82058945 None:intergenic 60.0%
AGTTGAGGAGTCCGACACGG+CGG - chr1.2:82058266-82058285 None:intergenic 60.0%
CACCCATGACGACGGTGAGA+GGG - chr1.2:82058893-82058912 None:intergenic 60.0%
GAGAAGGTGGCAGACCGGAT+GGG - chr1.2:82058927-82058946 None:intergenic 60.0%
GATCCTTCCGGTGGCATCTC+AGG + chr1.2:82058310-82058329 MS.gene065136:CDS 60.0%
GCAGAAGATGGGGGAGAAGG+TGG - chr1.2:82058940-82058959 None:intergenic 60.0%
GGAACCACCACCCATGACGA+CGG - chr1.2:82058901-82058920 None:intergenic 60.0%
TCACCTGAGATGCCACCGGA+AGG - chr1.2:82058316-82058335 None:intergenic 60.0%
TGCCACCGGAAGGATCTGCT+CGG - chr1.2:82058306-82058325 None:intergenic 60.0%
!! CGCCGCCCCCATTTTTCTGT+GGG + chr1.2:82058800-82058819 MS.gene065136:CDS 60.0%
!! GGGTTAGCTACTCTGCTCGG+CGG - chr1.2:82058829-82058848 None:intergenic 60.0%
!! TCGCCGCCCCCATTTTTCTG+TGG + chr1.2:82058799-82058818 MS.gene065136:CDS 60.0%
ACCCGAGCAGATCCTTCCGG+TGG + chr1.2:82058301-82058320 MS.gene065136:CDS 65.0%
CCACCCATGACGACGGTGAG+AGG - chr1.2:82058894-82058913 None:intergenic 65.0%
CCTCTCACCGTCGTCATGGG+TGG + chr1.2:82058891-82058910 MS.gene065136:CDS 65.0%
CTCACCGTCGTCATGGGTGG+TGG + chr1.2:82058894-82058913 MS.gene065136:CDS 65.0%
GAAAAATGGGGGCGGCGACG+AGG - chr1.2:82058797-82058816 None:intergenic 65.0%
GCCACCGGAAGGATCTGCTC+GGG - chr1.2:82058305-82058324 None:intergenic 65.0%
GCCCCTCTCACCGTCGTCAT+GGG + chr1.2:82058888-82058907 MS.gene065136:CDS 65.0%
GCGGGGCTTAGGGCAAACGA+CGG - chr1.2:82058247-82058266 None:intergenic 65.0%
GGAGAAGGTGGCAGACCGGA+TGG - chr1.2:82058928-82058947 None:intergenic 65.0%
GTGGTGGTTCCTGCCCCATC+CGG + chr1.2:82058910-82058929 MS.gene065136:CDS 65.0%
GTTGAGGAGTCCGACACGGC+GGG - chr1.2:82058265-82058284 None:intergenic 65.0%
TGGGGGAGAAGGTGGCAGAC+CGG - chr1.2:82058932-82058951 None:intergenic 65.0%
TTGAGGAGTCCGACACGGCG+GGG - chr1.2:82058264-82058283 None:intergenic 65.0%
AGTCCGACACGGCGGGGCTT+AGG - chr1.2:82058258-82058277 None:intergenic 70.0%
CGCCCCTCTCACCGTCGTCA+TGG + chr1.2:82058887-82058906 MS.gene065136:CDS 70.0%
GTCCGACACGGCGGGGCTTA+GGG - chr1.2:82058257-82058276 None:intergenic 70.0%
TGCCCTAAGCCCCGCCGTGT+CGG + chr1.2:82058252-82058271 MS.gene065136:CDS 70.0%
GGCGGCGACGAGGCTACTGG+AGG - chr1.2:82058787-82058806 None:intergenic 75.0%
GGTGGCAGACCGGATGGGGC+AGG - chr1.2:82058922-82058941 None:intergenic 75.0%
! GGGGGCGGCGACGAGGCTAC+TGG - chr1.2:82058790-82058809 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 82058228 82059074 82058228 ID=MS.gene065136
chr1.2 mRNA 82058228 82059074 82058228 ID=MS.gene065136.t1;Parent=MS.gene065136
chr1.2 exon 82058228 82058331 82058228 ID=MS.gene065136.t1.exon1;Parent=MS.gene065136.t1
chr1.2 CDS 82058228 82058331 82058228 ID=cds.MS.gene065136.t1;Parent=MS.gene065136.t1
chr1.2 exon 82058432 82058498 82058432 ID=MS.gene065136.t1.exon2;Parent=MS.gene065136.t1
chr1.2 CDS 82058432 82058498 82058432 ID=cds.MS.gene065136.t1;Parent=MS.gene065136.t1
chr1.2 exon 82058754 82059074 82058754 ID=MS.gene065136.t1.exon3;Parent=MS.gene065136.t1
chr1.2 CDS 82058754 82059074 82058754 ID=cds.MS.gene065136.t1;Parent=MS.gene065136.t1
Gene Sequence

>MS.gene065136

ATGAACGGAAGAGACTCCGTCGTTTGCCCTAAGCCCCGCCGTGTCGGACTCCTCAACTTCGCCGTCAACGACTACCCGAGCAGATCCTTCCGGTGGCATCTCAGGTGAATTTTAATTTCTCTCTGTTTTTCTTCTTTTCTCTTTTAATTTGCTTTGGTTCAATTTTATTAAACTTTTGCATTTTTCGTTTTTCTTCTTCTGCAGTTGCCAAGTTGAACCGTGTGATTCAAACTCATCTGCGTCAAATCCCTTGGACACAATTCTCACCAAGGTAAATAATTCCTCTATTATTTTATATGAATTTAGGTTAAAATTGGAATAATTTATCTATTATTTTATATGAATTTAGGTTAAATTTTAATAATTGATCTATTTTTAACATTGGTTAACGTTAACTTGTTACTTGTTAAAATTTGAATAGTTGATTGATTACAGTTAAAGCTTGAATTATTGTGCCTTTTGTTGAATTGCTTTGTGTTATGCTTTTTGATTGAATTGTTCTGTGTTGTGCTTTTGTGATTTGCAGGATGATGATTGTGATACGGAACAATCAAGTCCTCCAGTAGCCTCGTCGCCGCCCCCATTTTTCTGTGGGTCTCCGCCGAGCAGAGTAGCTAACCCACTAATTCAGGATGCTCGATTCGGGGATGAGAATTTCTCGCCCCTCTCACCGTCGTCATGGGTGGTGGTTCCTGCCCCATCCGGTCTGCCACCTTCTCCCCCATCTTCTGCAAGGAAGGGAGGATGTGTTCGGGCCAATTTTGGTAACAATCCGGCGGTGAGAGTCGAAGGATTCGATTGCCTTGACAGGGACAGCCGTAATTGCAGCATCCCTGCTCTGGCTTAG

Protein sequence

>MS.gene065136.t1

MNGRDSVVCPKPRRVGLLNFAVNDYPSRSFRWHLSCQVEPCDSNSSASNPLDTILTKDDDCDTEQSSPPVASSPPPFFCGSPPSRVANPLIQDARFGDENFSPLSPSSWVVVPAPSGLPPSPPSSARKGGCVRANFGNNPAVRVEGFDCLDRDSRNCSIPALA