AlfalfaGEDB | MS.gene05809

M. sativa cultivar XinJiangDaYe / MS.gene05809

KEGG: K02923
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01781.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05809.t1	XP_003593868.1	100	68	0	0	5	72	2	69	6.00E-28	132.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05809.t1	O22860	91.2	68	6	0	5	72	2	69	6.7e-28	124.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05809.t1	G7IP34	100.0	68	0	0	5	72	2	69	4.3e-28	132.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051063	MS.gene05809	0.816916	4.32E-52	-1.69E-46
MS.gene051850	MS.gene05809	0.803145	4.01E-49	-1.69E-46
MS.gene052202	MS.gene05809	0.803421	3.51E-49	-1.69E-46
MS.gene053109	MS.gene05809	0.805687	1.18E-49	-1.69E-46
MS.gene056432	MS.gene05809	0.819911	9.06E-53	-1.69E-46
MS.gene056760	MS.gene05809	0.815209	1.04E-51	-1.69E-46
MS.gene05809	MS.gene058906	0.840517	8.29E-58	-1.69E-46
MS.gene05809	MS.gene06001	0.841972	3.44E-58	-1.69E-46
MS.gene05809	MS.gene061145	0.826744	2.29E-54	-1.69E-46
MS.gene05809	MS.gene06172	0.827774	1.30E-54	-1.69E-46
MS.gene05809	MS.gene061966	0.811117	8.22E-51	-1.69E-46
MS.gene05809	MS.gene064071	0.814594	1.42E-51	-1.69E-46
MS.gene05809	MS.gene064072	0.817294	3.55E-52	-1.69E-46
MS.gene05809	MS.gene065136	-0.800618	1.32E-48	-1.69E-46
MS.gene05809	MS.gene067938	0.807777	4.28E-50	-1.69E-46
MS.gene05809	MS.gene06989	0.830915	2.24E-55	-1.69E-46
MS.gene05809	MS.gene070222	0.803661	3.13E-49	-1.69E-46
MS.gene05809	MS.gene07217	0.814037	1.89E-51	-1.69E-46
MS.gene05809	MS.gene07222	0.827163	1.82E-54	-1.69E-46
MS.gene05809	MS.gene073827	0.804514	2.08E-49	-1.69E-46
MS.gene05809	MS.gene074000	0.817699	2.88E-52	-1.69E-46
MS.gene05809	MS.gene074435	0.815101	1.10E-51	-1.69E-46
MS.gene05809	MS.gene09032	0.826269	2.98E-54	-1.69E-46
MS.gene05809	MS.gene20740	0.825068	5.73E-54	-1.69E-46
MS.gene05809	MS.gene20956	0.803973	2.70E-49	-1.69E-46
MS.gene05809	MS.gene21089	0.838871	2.22E-57	-1.69E-46
MS.gene05809	MS.gene22968	0.830404	2.99E-55	-1.69E-46
MS.gene05809	MS.gene23059	0.818518	1.88E-52	-1.69E-46
MS.gene05809	MS.gene26567	0.800411	1.46E-48	-1.69E-46
MS.gene05809	MS.gene26974	0.811798	5.85E-51	-1.69E-46
MS.gene05809	MS.gene27879	0.804273	2.34E-49	-1.69E-46
MS.gene05809	MS.gene27991	0.835276	1.84E-56	-1.69E-46
MS.gene05809	MS.gene28159	0.837413	5.28E-57	-1.69E-46
MS.gene05809	MS.gene30419	0.807262	5.51E-50	-1.69E-46
MS.gene05809	MS.gene35214	0.800227	1.59E-48	-1.69E-46
MS.gene05809	MS.gene44260	0.806751	7.06E-50	-1.69E-46
MS.gene05809	MS.gene47955	0.835833	1.33E-56	-1.69E-46
MS.gene05809	MS.gene55732	0.834859	2.35E-56	-1.69E-46
MS.gene05809	MS.gene55887	0.817448	3.28E-52	-1.69E-46
MS.gene05809	MS.gene58163	0.812842	3.46E-51	-1.69E-46
MS.gene05809	MS.gene66089	0.826321	2.89E-54	-1.69E-46
MS.gene05809	MS.gene68810	0.807117	5.91E-50	-1.69E-46
MS.gene05809	MS.gene70305	0.827745	1.32E-54	-1.69E-46
MS.gene05809	MS.gene74980	0.800752	1.24E-48	-1.69E-46
MS.gene05809	MS.gene75477	0.822014	2.97E-53	-1.69E-46
MS.gene05809	MS.gene76478	0.818991	1.47E-52	-1.69E-46
MS.gene05809	MS.gene83217	0.815649	8.30E-52	-1.69E-46
MS.gene05809	MS.gene88427	0.80086	1.18E-48	-1.69E-46
MS.gene05809	MS.gene89677	0.815664	8.23E-52	-1.69E-46
MS.gene05809	MS.gene89721	0.836556	8.73E-57	-1.69E-46
MS.gene05809	MS.gene91524	0.815109	1.09E-51	-1.69E-46
MS.gene05809	MS.gene92572	0.80138	9.26E-49	-1.69E-46
MS.gene05809	MS.gene97761	0.801613	8.29E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05809.t1	MTR_2g018680	100.000	68	0	5	72	2	69	1.66e-43	135
MS.gene05809.t1	MTR_4g068040	94.118	68	4	5	72	2	69	3.74e-41	129
MS.gene05809.t1	MTR_2g018680	100.000	63	0	5	67	2	64	6.99e-40	125
MS.gene05809.t1	MTR_8g079485	91.045	67	6	6	72	3	69	7.61e-39	123
MS.gene05809.t1	MTR_4g068040	95.161	62	3	5	66	2	63	2.17e-37	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05809.t1	AT3G59540	91.176	68	6	0	5	72	2	69	8.30e-40	125
MS.gene05809.t1	AT2G43460	91.176	68	6	0	5	72	2	69	8.30e-40	125

Find 16 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAGCAAATCAACGAGATTA+AGG	0.294566	2.3:+67910297	MS.gene05809:CDS
TGACCTCGCATCCTTCCTTC+TGG	0.376233	2.3:-67910335	None:intergenic
TGAGTGTTCAAGACCTTTAG+AGG	0.379570	2.3:+67911032	MS.gene05809:CDS
AGTGAGAATCAAGAGGAGTA+AGG	0.485084	2.3:+67910357	MS.gene05809:CDS
TAAGGATGTGGTTAAGTTCA+AGG	0.499148	2.3:+67910375	MS.gene05809:CDS
ATGTGGTTAAGTTCAAGGTC+AGG	0.518401	2.3:+67910380	MS.gene05809:CDS
AATCAAGAGGAGTAAGGATG+TGG	0.526249	2.3:+67910363	MS.gene05809:CDS
ACTAAGTGTGTAGAGGTACT+TGG	0.532503	2.3:-67910407	None:intergenic
TTAATCTCGTTGATTTGCTT+AGG	0.534022	2.3:-67910295	None:intergenic
CCTTCTCACTGCCAGAAGGA+AGG	0.537276	2.3:+67910324	MS.gene05809:CDS
TTGAAGCAATCACTTCCTCC+AGG	0.552717	2.3:+67910457	MS.gene05809:CDS
CCTTCCTTCTGGCAGTGAGA+AGG	0.565796	2.3:-67910324	None:intergenic
ATTTCCTTCTCACTGCCAGA+AGG	0.584170	2.3:+67910320	MS.gene05809:CDS
CGAGGTCAGTGAGAATCAAG+AGG	0.618562	2.3:+67910350	MS.gene05809:CDS
CTGCCAGAAGGAAGGATGCG+AGG	0.654921	2.3:+67910332	MS.gene05809:CDS
CAAACACACTAAGTGTGTAG+AGG	0.657075	2.3:-67910414	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTATATTTATTTAGCCT+AGG	+	chr2.3:67910505-67910524	MS.gene05809:intron	15.0%
!!!	ATTTTTAACTCAATTTATCT+TGG	+	chr2.3:67910692-67910711	MS.gene05809:intron	15.0%
!!	AAATTGATATGGTTAAAGAA+GGG	-	chr2.3:67910955-67910974	None:intergenic	20.0%
!!	CTTTATCATGTTACAATATT+TGG	-	chr2.3:67910863-67910882	None:intergenic	20.0%
!!	TAGTTCAATTGTCTATATTT+AGG	+	chr2.3:67910544-67910563	MS.gene05809:intron	20.0%
!!	TTACAATATTTGGTTACTAT+TGG	-	chr2.3:67910853-67910872	None:intergenic	20.0%
!!!	ATCCATTTTAATTTTCCAAA+AGG	-	chr2.3:67910924-67910943	None:intergenic	20.0%
!!!	TTCCTTTTGGAAAATTAAAA+TGG	+	chr2.3:67910919-67910938	MS.gene05809:intron	20.0%
!	ACATATAGCACAAATTGATA+TGG	-	chr2.3:67910966-67910985	None:intergenic	25.0%
!	ATTAAACTGTTGATACAAAC+AGG	-	chr2.3:67910571-67910590	None:intergenic	25.0%
!	CAAATTGATATGGTTAAAGA+AGG	-	chr2.3:67910956-67910975	None:intergenic	25.0%
!	TCAAATAAGTCAATTCAATC+CGG	+	chr2.3:67910819-67910838	MS.gene05809:intron	25.0%
!	TTAAACTGTTGATACAAACA+GGG	-	chr2.3:67910570-67910589	None:intergenic	25.0%
	AATTGAACTAGTTGAACTGT+TGG	-	chr2.3:67910534-67910553	None:intergenic	30.0%
	ATTTGGTTACTATTGGCAAA+AGG	-	chr2.3:67910846-67910865	None:intergenic	30.0%
	CTTATTTGAGGAACATATCT+TGG	-	chr2.3:67910808-67910827	None:intergenic	30.0%
	GATAAAGTTAACATTAGCAC+TGG	+	chr2.3:67910876-67910895	MS.gene05809:intron	30.0%
	TAAGCAAATCAACGAGATTA+AGG	+	chr2.3:67910297-67910316	MS.gene05809:CDS	30.0%
	TTTGGTTACTATTGGCAAAA+GGG	-	chr2.3:67910845-67910864	None:intergenic	30.0%
!	GGATTGAATTGACTTATTTG+AGG	-	chr2.3:67910820-67910839	None:intergenic	30.0%
!	TTTCTTCTATGTCTTCCTTT+TGG	+	chr2.3:67910906-67910925	MS.gene05809:intron	30.0%
!!	TTAATCTCGTTGATTTGCTT+AGG	-	chr2.3:67910298-67910317	None:intergenic	30.0%
	TAAACACTTGTGAGACTGTT+TGG	-	chr2.3:67910783-67910802	None:intergenic	35.0%
	TAAGGATGTGGTTAAGTTCA+AGG	+	chr2.3:67910375-67910394	MS.gene05809:CDS	35.0%
!	ACAATGCTCTTGATGTTTTC+AGG	+	chr2.3:67911007-67911026	MS.gene05809:intron	35.0%
	AATCAAGAGGAGTAAGGATG+TGG	+	chr2.3:67910363-67910382	MS.gene05809:CDS	40.0%
	AGTGAGAATCAAGAGGAGTA+AGG	+	chr2.3:67910357-67910376	MS.gene05809:CDS	40.0%
	AGTTGAACTGTTGGTATCCT+AGG	-	chr2.3:67910525-67910544	None:intergenic	40.0%
	ATGTGGTTAAGTTCAAGGTC+AGG	+	chr2.3:67910380-67910399	MS.gene05809:CDS	40.0%
	CAAACACACTAAGTGTGTAG+AGG	-	chr2.3:67910417-67910436	None:intergenic	40.0%
	TGTAAATCCAACTACAGACC+TGG	-	chr2.3:67910478-67910497	None:intergenic	40.0%
!!	ACTAAGTGTGTAGAGGTACT+TGG	-	chr2.3:67910410-67910429	None:intergenic	40.0%
	AAATCCAACTACAGACCTGG+AGG	-	chr2.3:67910475-67910494	None:intergenic	45.0%
	ATTTCCTTCTCACTGCCAGA+AGG	+	chr2.3:67910320-67910339	MS.gene05809:CDS	45.0%
	GTTACTATTGGCAAAAGGGC+CGG	-	chr2.3:67910841-67910860	None:intergenic	45.0%
	TTGAAGCAATCACTTCCTCC+AGG	+	chr2.3:67910457-67910476	MS.gene05809:CDS	45.0%
	ACTTCCTCCAGGTCTGTAGT+TGG	+	chr2.3:67910468-67910487	MS.gene05809:intron	50.0%
	CGAGGTCAGTGAGAATCAAG+AGG	+	chr2.3:67910350-67910369	MS.gene05809:CDS	50.0%
	CCTTCCTTCTGGCAGTGAGA+AGG	-	chr2.3:67910327-67910346	None:intergenic	55.0%
	CCTTCTCACTGCCAGAAGGA+AGG	+	chr2.3:67910324-67910343	MS.gene05809:CDS	55.0%
	TGACCTCGCATCCTTCCTTC+TGG	-	chr2.3:67910338-67910357	None:intergenic	55.0%
	CTGCCAGAAGGAAGGATGCG+AGG	+	chr2.3:67910332-67910351	MS.gene05809:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	67910225	67911051	67910225	ID=MS.gene05809
chr2.3	mRNA	67910225	67911051	67910225	ID=MS.gene05809.t1;Parent=MS.gene05809
chr2.3	exon	67910225	67910236	67910225	ID=MS.gene05809.t1.exon1;Parent=MS.gene05809.t1
chr2.3	CDS	67910225	67910236	67910225	ID=cds.MS.gene05809.t1;Parent=MS.gene05809.t1
chr2.3	exon	67910295	67910478	67910295	ID=MS.gene05809.t1.exon2;Parent=MS.gene05809.t1
chr2.3	CDS	67910295	67910478	67910295	ID=cds.MS.gene05809.t1;Parent=MS.gene05809.t1
chr2.3	exon	67911029	67911051	67911029	ID=MS.gene05809.t1.exon3;Parent=MS.gene05809.t1
chr2.3	CDS	67911029	67911051	67911029	ID=cds.MS.gene05809.t1;Parent=MS.gene05809.t1

Gene Sequence

>MS.gene05809

ATGTTTGCTGATGTATTACTATGTCTATTTGTGCTGATTGTTCTGACGATGTATTTGTTGTTGTACATAGCCTAAGCAAATCAACGAGATTAAGGATTTCCTTCTCACTGCCAGAAGGAAGGATGCGAGGTCAGTGAGAATCAAGAGGAGTAAGGATGTGGTTAAGTTCAAGGTCAGGTGTTCCAAGTACCTCTACACACTTAGTGTGTTTGACGTTGAGAAAGCTGACAAATTGAAGCAATCACTTCCTCCAGGTCTGTAGTTGGATTTACATTTTGAGATTTTATATTTATTTAGCCTAGGATACCAACAGTTCAACTAGTTCAATTGTCTATATTTAGGCCCTGTTTGTATCAACAGTTTAATTTGTAGTTTACAGCTTAAGCAGTTATCATCATATGCGCTTATGTATAAGCTATTTCTATAACAGAAGATAGATTAGTTAAATTGTTTGCGTTGAAGCTATAATTTTTAACTCAATTTATCTTGGAGAACTTATACAAACAAGCTGAAATCAGTTTATGAACATGTCATAAGCTGTTTTCATAAGTTCTTCCAAACAGTCTCACAAGTGTTTATGCCAAGATATGTTCCTCAAATAAGTCAATTCAATCCGGCCCTTTTGCCAATAGTAACCAAATATTGTAACATGATAAAGTTAACATTAGCACTGGCTATATGTTTCTTCTATGTCTTCCTTTTGGAAAATTAAAATGGATTATGTATGCCCTTCTTTAACCATATCAATTTGTGCTATATGTAATCTTGTTGTTTTGTGTGTCACAATGCTCTTGATGTTTTCAGGTTTGAGTGTTCAAGACCTTTAG

Protein sequence

>MS.gene05809.t1

MFADPKQINEIKDFLLTARRKDARSVRIKRSKDVVKFKVRCSKYLYTLSVFDVEKADKLKQSLPPGLSVQDL