AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92397

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92397.t1 MTR_8g037890 89.803 304 23 1 1 296 1 304 0.0 552
MS.gene92397.t1 MTR_8g056820 86.561 253 34 0 1 253 1 253 1.61e-162 453
MS.gene92397.t1 MTR_5g022310 75.578 303 55 5 1 298 1 289 4.73e-158 444
MS.gene92397.t1 MTR_5g022310 83.465 254 38 2 1 250 1 254 1.34e-152 428
MS.gene92397.t1 MTR_8g075550 64.901 302 80 5 1 293 1 285 6.73e-129 370
MS.gene92397.t1 MTR_1g025420 61.818 275 101 3 14 285 11 284 4.35e-117 341
MS.gene92397.t1 MTR_1g025420 67.220 241 75 3 14 251 11 250 3.67e-114 331
MS.gene92397.t1 MTR_3g114030 68.220 236 71 3 20 252 1 235 9.95e-113 326
MS.gene92397.t1 MTR_4g073730 61.240 258 91 3 11 260 5 261 7.16e-111 323
MS.gene92397.t1 MTR_4g073730 61.240 258 91 3 11 260 5 261 1.73e-110 324
MS.gene92397.t1 MTR_4g073720 56.818 264 98 6 9 270 9 258 5.33e-91 275
MS.gene92397.t1 MTR_5g022350 52.364 275 121 5 6 280 12 276 3.08e-89 270
MS.gene92397.t1 MTR_8g075510 54.839 248 101 6 4 250 10 247 3.79e-84 254
MS.gene92397.t1 MTR_2g068030 52.941 221 100 3 28 246 25 243 2.01e-82 250
MS.gene92397.t1 MTR_2g067980 54.274 234 101 3 24 253 22 253 3.44e-82 268
MS.gene92397.t1 MTR_8g075510 53.008 266 112 7 4 268 10 263 1.19e-81 250
MS.gene92397.t1 MTR_2g069660 53.527 241 104 4 9 247 9 243 2.37e-81 247
MS.gene92397.t1 MTR_7g102380 49.796 245 117 5 7 249 3 243 1.66e-80 244
MS.gene92397.t1 MTR_8g036215 51.046 239 112 4 9 246 3 237 7.21e-80 243
MS.gene92397.t1 MTR_1g021945 54.148 229 99 4 25 251 25 249 1.46e-78 240
MS.gene92397.t1 MTR_3g111620 53.275 229 101 5 25 251 26 250 6.00e-77 236
MS.gene92397.t1 MTR_2g068655 50.655 229 111 2 22 249 27 254 6.84e-77 238
MS.gene92397.t1 MTR_1g062660 51.464 239 109 5 13 249 11 244 3.00e-76 234
MS.gene92397.t1 MTR_1g062590 48.583 247 119 5 3 247 2 242 8.24e-76 233
MS.gene92397.t1 MTR_6g079580 50.204 245 109 5 11 247 1 240 1.67e-75 232
MS.gene92397.t1 MTR_1g062340 51.502 233 106 5 19 249 17 244 2.47e-75 231
MS.gene92397.t1 MTR_1g062350 50.403 248 115 6 4 249 3 244 4.89e-75 231
MS.gene92397.t1 MTR_1g062370 49.597 248 117 6 4 249 3 244 9.90e-75 230
MS.gene92397.t1 MTR_8g107140 49.600 250 118 5 1 247 1 245 1.91e-74 229
MS.gene92397.t1 MTR_1g062630 49.597 248 117 6 3 248 2 243 1.24e-71 223
MS.gene92397.t1 MTR_5g023850 47.059 255 96 4 1 251 1 220 7.57e-71 219
MS.gene92397.t1 MTR_5g059200 47.200 250 117 7 1 246 1 239 1.93e-69 218
MS.gene92397.t1 MTR_7g062610 45.492 244 129 3 5 247 2 242 1.70e-68 214
MS.gene92397.t1 MTR_3g068015 48.000 225 112 4 25 247 27 248 6.94e-68 213
MS.gene92397.t1 MTR_1g062690 45.565 248 103 7 4 249 3 220 1.96e-61 195
MS.gene92397.t1 MTR_2g067990 44.922 256 111 9 19 271 20 248 5.07e-60 207
MS.gene92397.t1 MTR_6g009480 40.239 251 146 4 1 248 4 253 3.10e-59 191
MS.gene92397.t1 MTR_4g063630 42.739 241 126 8 13 246 16 251 9.72e-59 189
MS.gene92397.t1 MTR_5g010640 40.392 255 125 9 1 247 1 236 6.35e-50 166
MS.gene92397.t1 MTR_2g067970 45.894 207 106 6 69 271 3 207 3.49e-49 176
MS.gene92397.t1 MTR_8g096900 40.586 239 116 9 11 247 34 248 2.18e-44 152
MS.gene92397.t1 MTR_5g010635 40.969 227 110 9 23 247 21 225 4.22e-44 151
MS.gene92397.t1 MTR_5g022350 51.408 142 63 3 11 150 79 216 6.60e-39 137
MS.gene92397.t1 MTR_2g063150 34.742 213 130 5 26 236 34 239 1.22e-37 135
MS.gene92397.t1 MTR_8g089020 50.000 130 60 2 110 239 32 156 7.49e-37 130
MS.gene92397.t1 MTR_1g062390 31.274 259 157 8 1 253 1 244 2.19e-32 120
MS.gene92397.t1 MTR_7g076360 35.849 212 72 4 25 236 7 154 1.10e-31 117
MS.gene92397.t1 MTR_8g096910 44.604 139 61 6 9 141 1 129 2.54e-28 108
MS.gene92397.t1 MTR_8g096920 41.606 137 69 5 9 143 1 128 1.75e-23 95.5
MS.gene92397.t1 MTR_3g081550 47.368 95 47 2 111 203 16 109 1.93e-18 80.1
MS.gene92397.t1 MTR_1g062640 38.889 90 52 2 79 168 60 146 2.10e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92397.t1 AT4G36010 69.620 237 66 3 22 252 19 255 1.83e-117 341
MS.gene92397.t1 AT4G36010 69.620 237 66 3 22 252 19 255 1.83e-117 341
MS.gene92397.t1 AT2G17860 63.855 249 85 3 7 250 4 252 1.25e-111 324
MS.gene92397.t1 AT1G75800 59.770 261 96 5 9 263 5 262 7.18e-107 315
MS.gene92397.t1 AT4G38670 61.694 248 90 4 9 252 6 252 1.51e-105 310
MS.gene92397.t1 AT4G38670 61.694 248 90 4 9 252 6 252 1.51e-105 310
MS.gene92397.t1 AT4G38670 65.652 230 74 4 27 252 8 236 7.60e-105 307
MS.gene92397.t1 AT1G20030 63.983 236 79 3 22 252 16 250 3.19e-104 308
MS.gene92397.t1 AT1G20030 64.935 231 75 3 27 252 4 233 4.25e-104 307
MS.gene92397.t1 AT4G38660 56.506 269 111 3 7 275 11 273 2.55e-98 294
MS.gene92397.t1 AT4G24180 56.923 260 101 5 6 258 7 262 2.47e-96 287
MS.gene92397.t1 AT4G38660 57.812 256 102 3 20 275 2 251 2.66e-96 288
MS.gene92397.t1 AT4G24180 58.400 250 93 5 6 248 7 252 2.84e-96 285
MS.gene92397.t1 AT4G24180 57.031 256 98 5 11 258 16 267 3.65e-96 287
MS.gene92397.t1 AT4G24180 58.537 246 90 5 11 248 16 257 6.91e-96 285
MS.gene92397.t1 AT5G24620 47.569 288 132 5 9 278 7 293 1.32e-85 264
MS.gene92397.t1 AT4G24180 56.410 234 91 5 6 232 7 236 2.94e-85 259
MS.gene92397.t1 AT5G24620 47.569 288 132 5 9 278 62 348 3.81e-85 264
MS.gene92397.t1 AT5G24620 47.569 288 132 5 9 278 83 369 5.01e-85 265
MS.gene92397.t1 AT4G24180 56.522 230 88 5 11 232 16 241 1.10e-84 258
MS.gene92397.t1 AT5G38280 48.627 255 126 3 8 257 6 260 1.12e-83 266
MS.gene92397.t1 AT5G38280 49.004 251 124 2 11 257 10 260 2.22e-83 265
MS.gene92397.t1 AT1G75030 52.191 251 109 7 6 250 1 246 1.14e-82 250
MS.gene92397.t1 AT1G19320 51.394 251 112 5 1 246 1 246 4.60e-82 249
MS.gene92397.t1 AT1G75050 53.061 245 107 6 7 248 5 244 5.93e-81 246
MS.gene92397.t1 AT1G75040 53.942 241 105 4 7 247 5 239 1.37e-80 245
MS.gene92397.t1 AT1G18250 51.982 227 102 4 23 247 19 240 1.03e-76 235
MS.gene92397.t1 AT1G18250 52.000 225 101 4 25 247 20 239 1.73e-76 234
MS.gene92397.t1 AT1G73620 52.444 225 100 4 25 247 41 260 2.01e-76 235
MS.gene92397.t1 AT1G77700 48.221 253 117 6 12 260 43 285 5.42e-76 236
MS.gene92397.t1 AT1G77700 49.793 241 107 6 12 248 43 273 1.59e-75 235
MS.gene92397.t1 AT1G77700 52.232 224 97 5 25 248 88 301 6.31e-75 234
MS.gene92397.t1 AT5G02140 42.857 252 138 3 7 257 5 251 6.25e-66 209
MS.gene92397.t1 AT4G36000 53.589 209 69 4 102 310 1 181 6.98e-66 206
MS.gene92397.t1 AT4G36000 53.589 209 69 4 102 310 1 181 6.98e-66 206
MS.gene92397.t1 AT5G02140 44.215 242 129 3 7 247 5 241 1.54e-65 206
MS.gene92397.t1 AT4G18253 43.725 247 128 4 9 252 5 243 6.16e-64 202
MS.gene92397.t1 AT2G28790 44.628 242 125 7 10 246 11 248 4.13e-61 195
MS.gene92397.t1 AT2G28790 44.628 242 125 7 10 246 36 273 4.72e-61 196
MS.gene92397.t1 AT4G18250 41.606 274 150 6 5 276 35 300 8.54e-60 203
MS.gene92397.t1 AT1G70250 41.091 275 138 10 25 285 144 408 5.82e-59 202
MS.gene92397.t1 AT5G40020 42.128 235 131 4 23 255 25 256 7.95e-59 190
MS.gene92397.t1 AT4G11650 39.483 271 137 9 1 271 1 244 1.07e-52 174
MS.gene92397.t1 AT5G24620 49.383 162 66 2 133 278 15 176 5.09e-45 155
MS.gene92397.t1 AT2G24810 59.740 77 31 0 170 246 116 192 1.95e-27 106

Find 107 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGGTATTGGAAGAGAATT+AGG 0.170473 8.4:-60341684 None:intergenic
CCAAGTGGTCAGGCCGTCTA+TGG 0.217701 8.4:+60341854 MS.gene92397:CDS
CCAACAAGTTCGATCGATTC+CGG 0.259312 8.4:+60342432 MS.gene92397:CDS
CCAACAAGTTCGATCGATTC+CGG 0.259312 8.4:+60343105 MS.gene92397:CDS
CCTGAGGAGATGGATATATA+TGG 0.299759 8.4:+60342474 MS.gene92397:CDS
ATCTCAAGTTCGACCCAATC+TGG 0.307783 8.4:+60343069 MS.gene92397:CDS
ACCTCAAGTTCGACCCAATC+TGG 0.309245 8.4:+60342396 MS.gene92397:CDS
GAACTTGGCTTGCATGTATC+TGG 0.329096 8.4:-60342252 None:intergenic
GGAAAATTCTCCTGTGTTAC+CGG 0.333338 8.4:+60341907 MS.gene92397:CDS
AAAGCGCATGTGAAGCGTTT+GGG 0.344124 8.4:+60342193 MS.gene92397:CDS
CCATGTGGTTCTATCGTCAT+TGG 0.348717 8.4:-60342063 None:intergenic
ATTCTCTTCCAATACCTAAC+TGG 0.364848 8.4:+60341688 MS.gene92397:CDS
CAACAAGTTCGATCGATTCC+GGG 0.377626 8.4:+60342433 MS.gene92397:CDS
CAACAAGTTCGATCGATTCC+GGG 0.377626 8.4:+60343106 MS.gene92397:CDS
AACTCAATCAACCATCGCTT+TGG 0.382050 8.4:+60341657 MS.gene92397:CDS
TGTAGAGAACGGTGTTGTTC+CGG 0.383816 8.4:-60341782 None:intergenic
CCGGCGCCGGATAAAATTCC+AGG 0.384166 8.4:-60341763 None:intergenic
CATATGAACCAGTTAGGTAT+TGG 0.386121 8.4:-60341696 None:intergenic
GAGATTTCTCGTTCCCAGAT+TGG 0.387686 8.4:-60342410 None:intergenic
GAGATTTCTCGTTCCCAGAT+TGG 0.387686 8.4:-60343083 None:intergenic
CAATGGAGATTTCTCGTTCC+CGG 0.398071 8.4:-60342451 None:intergenic
CAATGGAGATTTCTCGTTCC+CGG 0.398071 8.4:-60343124 None:intergenic
CCGGAATCGATCGAACTTGT+TGG 0.416174 8.4:-60342432 None:intergenic
CCGGAATCGATCGAACTTGT+TGG 0.416174 8.4:-60343105 None:intergenic
TTAGCCGAGTTTACACTTAA+CGG 0.420790 8.4:+60341988 MS.gene92397:CDS
CTCATCACTTTCAACTCGGT+TGG 0.422630 8.4:-60342147 None:intergenic
AGATTTCTCGTTCCCAGATT+GGG 0.426200 8.4:-60342409 None:intergenic
AGATTTCTCGTTCCCAGATT+GGG 0.426200 8.4:-60343082 None:intergenic
AGCAGCAATCATAACATTGT+TGG 0.429720 8.4:-60342517 None:intergenic
GGGAGGTACGGCAAGGGCAT+TGG 0.430183 8.4:-60341833 None:intergenic
GCCGTACCTCCCAAGTGGTC+AGG 0.435086 8.4:+60341844 MS.gene92397:CDS
TGTCATGCAGTTTCCGCCTC+CGG 0.443562 8.4:-60342091 None:intergenic
GGCAAGGGCATTGGATTCGC+CGG 0.444861 8.4:-60341824 None:intergenic
CAAGTGGTCAGGCCGTCTAT+GGG 0.446135 8.4:+60341855 MS.gene92397:CDS
CCATAGACGGCCTGACCACT+TGG 0.446964 8.4:-60341854 None:intergenic
TTGTTGCATATGAACCAGTT+AGG 0.449950 8.4:-60341702 None:intergenic
GCCGGGTTGGAGAGCAAAGC+CGG 0.453151 8.4:-60341806 None:intergenic
CTGAGGAGATGGATATATAT+GGG 0.457856 8.4:+60342475 MS.gene92397:CDS
ACAACACCGTTCTCTACAAC+CGG 0.459009 8.4:+60341787 MS.gene92397:CDS
ATCTCCATTGCCTGAGGAGA+TGG 0.461012 8.4:+60342464 MS.gene92397:CDS
TGCAACTACACCGTTTGGCC+TGG 0.463035 8.4:+60341745 MS.gene92397:CDS
GTTTCCTCCAGCACACTCTA+TGG 0.465520 8.4:-60341947 None:intergenic
ACGAGTGCAACTACACCGTT+TGG 0.467155 8.4:+60341740 MS.gene92397:CDS
ATAGAACCACATGGAGCAAC+CGG 0.467179 8.4:+60342072 MS.gene92397:CDS
TAGGTATTGGAAGAGAATTA+GGG 0.467250 8.4:-60341683 None:intergenic
GGCGGAAACTGCATGACAAC+AGG 0.472230 8.4:+60342096 MS.gene92397:CDS
TATAGCTACGCTTATGATGA+TGG 0.472777 8.4:+60342312 MS.gene92397:CDS
GCCTGACCACTTGGGAGGTA+CGG 0.475859 8.4:-60341845 None:intergenic
AAAAGCGCATGTGAAGCGTT+TGG 0.476727 8.4:+60342192 MS.gene92397:CDS
TCCTCCACCATAGAGTGTGC+TGG 0.480343 8.4:+60341940 MS.gene92397:CDS
CCCAGATTGGGTCGAACTTG+AGG 0.481684 8.4:-60342397 None:intergenic
CCACATGGAGCAACCGGAGG+CGG 0.490132 8.4:+60342078 MS.gene92397:CDS
TTTACACTTAACGGTGCAAA+CGG 0.497247 8.4:+60341997 MS.gene92397:CDS
CATGTGGTTCTATCGTCATT+GGG 0.500356 8.4:-60342062 None:intergenic
GAACGGTGTTGTTCCGGCGC+CGG 0.511342 8.4:-60341776 None:intergenic
GAACTTGAGGTCGAACTTGA+GGG 0.522077 8.4:-60342384 None:intergenic
GCAAGGGCATTGGATTCGCC+GGG 0.523677 8.4:-60341823 None:intergenic
GGATAAAATTCCAGGCCAAA+CGG 0.531186 8.4:-60341755 None:intergenic
CCATATATATCCATCTCCTC+AGG 0.534013 8.4:-60342474 None:intergenic
ATATCCATCTCCTCAGGCAA+TGG 0.536781 8.4:-60342468 None:intergenic
ATATCCATCTCCTCAGGCAA+TGG 0.536781 8.4:-60343141 None:intergenic
GGGCATTGGATTCGCCGGGT+TGG 0.537166 8.4:-60341819 None:intergenic
TTTCTTCGACGTTAGTCTCG+TGG 0.547119 8.4:+60342026 MS.gene92397:CDS
TCTCAAGTTCGACCCAATCT+GGG 0.550401 8.4:+60343070 MS.gene92397:CDS
CCTCAAGTTCGACCCAATCT+GGG 0.550513 8.4:+60342397 MS.gene92397:CDS
GATCCGCAATATTGTTGTAG+TGG 0.551927 8.4:+60342216 MS.gene92397:CDS
CGAACTTGAGGTCGAACTTG+AGG 0.552011 8.4:-60342385 None:intergenic
CCGCCTCCGGTTGCTCCATG+TGG 0.564023 8.4:-60342078 None:intergenic
ACCACTTGGGAGGTACGGCA+AGG 0.566696 8.4:-60341840 None:intergenic
TCCAGCACACTCTATGGTGG+AGG 0.567323 8.4:-60341941 None:intergenic
CTCTGCTCTCAAGATTCCAC+CGG 0.569324 8.4:+60341886 MS.gene92397:CDS
AAGCGCATGTGAAGCGTTTG+GGG 0.569335 8.4:+60342194 MS.gene92397:CDS
GTAAACTCGGCTAATGTAGC+CGG 0.573023 8.4:-60341979 None:intergenic
CTGCATGACAACAGGTTGCA+TGG 0.576620 8.4:+60342104 MS.gene92397:CDS
GGTGGAGGAAGCACAGTCAC+CGG 0.577174 8.4:-60341926 None:intergenic
TGCACCGTTAAGTGTAAACT+CGG 0.588205 8.4:-60341992 None:intergenic
ATTACTCATCACTTTCAACT+CGG 0.591426 8.4:-60342151 None:intergenic
CATAGACGGCCTGACCACTT+GGG 0.591730 8.4:-60341853 None:intergenic
AGAGGGTACGACCCCATAGA+CGG 0.601389 8.4:-60341867 None:intergenic
TGAGGAGATGGATATATATG+GGG 0.605444 8.4:+60342476 MS.gene92397:CDS
CGGTGGAATCTTGAGAGCAG+AGG 0.605968 8.4:-60341885 None:intergenic
AGCACAGTCACCGGTAACAC+AGG 0.609119 8.4:-60341917 None:intergenic
GCAAAGCCGGTTGTAGAGAA+CGG 0.610915 8.4:-60341793 None:intergenic
TCCTCCAGCACACTCTATGG+TGG 0.611738 8.4:-60341944 None:intergenic
ACCGGCTTTGCTCTCCAACC+CGG 0.615393 8.4:+60341805 MS.gene92397:CDS
GAGTTGAAAGTGATGAGTAA+TGG 0.615914 8.4:+60342153 MS.gene92397:CDS
TGGAGGAAACGCTCAACCAC+CGG 0.617523 8.4:+60341960 MS.gene92397:CDS
TTCGACGTTAGTCTCGTGGA+CGG 0.621410 8.4:+60342030 MS.gene92397:CDS
GGTGGAATCTTGAGAGCAGA+GGG 0.624367 8.4:-60341884 None:intergenic
AATTAGGGTAACCAAAGCGA+TGG 0.636779 8.4:-60341668 None:intergenic
AACTCGGCTAATGTAGCCGG+TGG 0.641121 8.4:-60341976 None:intergenic
AGACGGCCTGACCACTTGGG+AGG 0.643248 8.4:-60341850 None:intergenic
AAGTGGTCAGGCCGTCTATG+GGG 0.643584 8.4:+60341856 MS.gene92397:CDS
TCCACCATAGAGTGTGCTGG+AGG 0.646324 8.4:+60341943 MS.gene92397:CDS
CCACTTGGGAGGTACGGCAA+GGG 0.646374 8.4:-60341839 None:intergenic
GTGGTTCTATCGTCATTGGG+AGG 0.650114 8.4:-60342059 None:intergenic
CCCTTGCCGTACCTCCCAAG+TGG 0.650312 8.4:+60341839 MS.gene92397:CDS
CGAGAAATCTCCATTGCCTG+AGG 0.654237 8.4:+60342458 MS.gene92397:CDS
CGAGAAATCTCCATTGCCTG+AGG 0.654237 8.4:+60343131 MS.gene92397:CDS
TTGAAAGTGATGAGTAATGG+CGG 0.675133 8.4:+60342156 MS.gene92397:CDS
GAGGAGATGGATATATATGG+GGG 0.683840 8.4:+60342477 MS.gene92397:CDS
GAACCACATGGAGCAACCGG+AGG 0.692391 8.4:+60342075 MS.gene92397:CDS
TAAGCGTAGCTATAAGCGCG+AGG 0.692743 8.4:-60342303 None:intergenic
AAAGTGATGAGTAATGGCGG+AGG 0.693219 8.4:+60342159 MS.gene92397:CDS
GCGCCACTACAACAATATTG+CGG 0.704004 8.4:-60342219 None:intergenic
CCAATGACGATAGAACCACA+TGG 0.737275 8.4:+60342063 MS.gene92397:CDS
CATGACAACAGGTTGCATGG+TGG 0.757398 8.4:+60342107 MS.gene92397:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTACGTTCAAATATAATAAT+CGG + chr8.4:60342682-60342701 MS.gene92397:intron 20.0%
!!! ATTTAGCAGAATTTTTTGGA+TGG - chr8.4:60342890-60342909 None:intergenic 25.0%
!!! GAGAATTTAGCAGAATTTTT+TGG - chr8.4:60342894-60342913 None:intergenic 25.0%
ATTACTCATCACTTTCAACT+CGG - chr8.4:60342154-60342173 None:intergenic 30.0%
ATTATATTAAAAGCCCAACG+TGG + chr8.4:60342794-60342813 MS.gene92397:intron 30.0%
TTCGTGACTTTCAAAAAACT+AGG + chr8.4:60342616-60342635 MS.gene92397:intron 30.0%
TTTCAAAAAACTAGGTACGT+GGG + chr8.4:60342624-60342643 MS.gene92397:intron 30.0%
TTTGGATGGAATAGATCAAT+GGG - chr8.4:60342876-60342895 None:intergenic 30.0%
! TTTTGGATGGAATAGATCAA+TGG - chr8.4:60342877-60342896 None:intergenic 30.0%
!! TAGGTATTGGAAGAGAATTA+GGG - chr8.4:60341686-60341705 None:intergenic 30.0%
!! TTAGGTATTGGAAGAGAATT+AGG - chr8.4:60341687-60341706 None:intergenic 30.0%
!!! TTGTTTTGATCATAGTCACA+CGG + chr8.4:60342550-60342569 MS.gene92397:intron 30.0%
AAAAACTGCGAGTATGAACT+TGG - chr8.4:60342270-60342289 None:intergenic 35.0%
AGCAGCAATCATAACATTGT+TGG - chr8.4:60342520-60342539 None:intergenic 35.0%
ATTCTCTTCCAATACCTAAC+TGG + chr8.4:60341688-60341707 MS.gene92397:CDS 35.0%
CATATGAACCAGTTAGGTAT+TGG - chr8.4:60341699-60341718 None:intergenic 35.0%
CTGAGGAGATGGATATATAT+GGG + chr8.4:60342475-60342494 MS.gene92397:CDS 35.0%
CTTTCAAAAAACTAGGTACG+TGG + chr8.4:60342623-60342642 MS.gene92397:intron 35.0%
GAGTTGAAAGTGATGAGTAA+TGG + chr8.4:60342153-60342172 MS.gene92397:CDS 35.0%
TATAGCTACGCTTATGATGA+TGG + chr8.4:60342312-60342331 MS.gene92397:CDS 35.0%
TGAGGAGATGGATATATATG+GGG + chr8.4:60342476-60342495 MS.gene92397:CDS 35.0%
TTAGCCGAGTTTACACTTAA+CGG + chr8.4:60341988-60342007 MS.gene92397:CDS 35.0%
TTCAAAAAACTAGGTACGTG+GGG + chr8.4:60342625-60342644 MS.gene92397:intron 35.0%
TTGAAAGTGATGAGTAATGG+CGG + chr8.4:60342156-60342175 MS.gene92397:CDS 35.0%
TTGTTGCATATGAACCAGTT+AGG - chr8.4:60341705-60341724 None:intergenic 35.0%
TTTACACTTAACGGTGCAAA+CGG + chr8.4:60341997-60342016 MS.gene92397:CDS 35.0%
AACTCAATCAACCATCGCTT+TGG + chr8.4:60341657-60341676 MS.gene92397:CDS 40.0%
AATTAGGGTAACCAAAGCGA+TGG - chr8.4:60341671-60341690 None:intergenic 40.0%
ACTTGAGGGACAAAAAGTGA+TGG - chr8.4:60342373-60342392 None:intergenic 40.0%
AGATTTCTCGTTCCCAGATT+GGG - chr8.4:60342412-60342431 None:intergenic 40.0%
CCATATATATCCATCTCCTC+AGG - chr8.4:60342477-60342496 None:intergenic 40.0%
CCTGAGGAGATGGATATATA+TGG + chr8.4:60342474-60342493 MS.gene92397:CDS 40.0%
GAACTTGAGATCGAACTTGA+GGG - chr8.4:60343060-60343079 None:intergenic 40.0%
GAGGAGATGGATATATATGG+GGG + chr8.4:60342477-60342496 MS.gene92397:CDS 40.0%
GATCCGCAATATTGTTGTAG+TGG + chr8.4:60342216-60342235 MS.gene92397:CDS 40.0%
GGAAAATTCTCCTGTGTTAC+CGG + chr8.4:60341907-60341926 MS.gene92397:CDS 40.0%
GGATAAAATTCCAGGCCAAA+CGG - chr8.4:60341758-60341777 None:intergenic 40.0%
TCAAAAAACTAGGTACGTGG+GGG + chr8.4:60342626-60342645 MS.gene92397:intron 40.0%
TGCACCGTTAAGTGTAAACT+CGG - chr8.4:60341995-60342014 None:intergenic 40.0%
! CAATGGGTGTGTGTTTCTAT+TGG - chr8.4:60342860-60342879 None:intergenic 40.0%
! CATGTGGTTCTATCGTCATT+GGG - chr8.4:60342065-60342084 None:intergenic 40.0%
! GGTAACACAGGAGAATTTTC+CGG - chr8.4:60341908-60341927 None:intergenic 40.0%
! GTTTGGCCTGGAATTTTATC+CGG + chr8.4:60341757-60341776 MS.gene92397:CDS 40.0%
AAAGTGATGAGTAATGGCGG+AGG + chr8.4:60342159-60342178 MS.gene92397:CDS 45.0%
AATTCGTACCACGTACCGAA+GGG - chr8.4:60342585-60342604 None:intergenic 45.0%
ACAACACCGTTCTCTACAAC+CGG + chr8.4:60341787-60341806 MS.gene92397:CDS 45.0%
ACAGAGTCGGTACGCTATTT+GGG + chr8.4:60342653-60342672 MS.gene92397:intron 45.0%
ATAGAACCACATGGAGCAAC+CGG + chr8.4:60342072-60342091 MS.gene92397:CDS 45.0%
ATATCCATCTCCTCAGGCAA+TGG - chr8.4:60342471-60342490 None:intergenic 45.0%
ATCTCAAGTTCGACCCAATC+TGG + chr8.4:60343069-60343088 MS.gene92397:CDS 45.0%
CAAAAAACTAGGTACGTGGG+GGG + chr8.4:60342627-60342646 MS.gene92397:intron 45.0%
CAATGGAGATTTCTCGTTCC+CGG - chr8.4:60342454-60342473 None:intergenic 45.0%
CCAATGACGATAGAACCACA+TGG + chr8.4:60342063-60342082 MS.gene92397:CDS 45.0%
CGAACTTGAGATCGAACTTG+AGG - chr8.4:60343061-60343080 None:intergenic 45.0%
CTCATCACTTTCAACTCGGT+TGG - chr8.4:60342150-60342169 None:intergenic 45.0%
GAACTTGAGGTCGAACTTGA+GGG - chr8.4:60342387-60342406 None:intergenic 45.0%
GAGATTTCTCGTTCCCAGAT+TGG - chr8.4:60342413-60342432 None:intergenic 45.0%
GCGCCACTACAACAATATTG+CGG - chr8.4:60342222-60342241 None:intergenic 45.0%
GTAAACTCGGCTAATGTAGC+CGG - chr8.4:60341982-60342001 None:intergenic 45.0%
TACAGAGTCGGTACGCTATT+TGG + chr8.4:60342652-60342671 MS.gene92397:intron 45.0%
TCTCAAGTTCGACCCAATCT+GGG + chr8.4:60343070-60343089 MS.gene92397:CDS 45.0%
TGCTGTGATTGAACCACGTT+GGG - chr8.4:60342810-60342829 None:intergenic 45.0%
TTCGGTACGTGGTACGAATT+CGG + chr8.4:60342585-60342604 MS.gene92397:intron 45.0%
! AAAAGCGCATGTGAAGCGTT+TGG + chr8.4:60342192-60342211 MS.gene92397:CDS 45.0%
! AAAGCGCATGTGAAGCGTTT+GGG + chr8.4:60342193-60342212 MS.gene92397:CDS 45.0%
! AACACAGGAGAATTTTCCGG+TGG - chr8.4:60341905-60341924 None:intergenic 45.0%
! CAACAAGTTCGATCGATTCC+GGG + chr8.4:60342433-60342452 MS.gene92397:CDS 45.0%
! CCAACAAGTTCGATCGATTC+CGG + chr8.4:60342432-60342451 MS.gene92397:CDS 45.0%
! CCATGTGGTTCTATCGTCAT+TGG - chr8.4:60342066-60342085 None:intergenic 45.0%
! TTTCTTCGACGTTAGTCTCG+TGG + chr8.4:60342026-60342045 MS.gene92397:CDS 45.0%
!! GAACTTGGCTTGCATGTATC+TGG - chr8.4:60342255-60342274 None:intergenic 45.0%
!! TGTAGAGAACGGTGTTGTTC+CGG - chr8.4:60341785-60341804 None:intergenic 45.0%
ACCTCAAGTTCGACCCAATC+TGG + chr8.4:60342396-60342415 MS.gene92397:CDS 50.0%
ACGAGTGCAACTACACCGTT+TGG + chr8.4:60341740-60341759 MS.gene92397:CDS 50.0%
ATCTCCATTGCCTGAGGAGA+TGG + chr8.4:60342464-60342483 MS.gene92397:CDS 50.0%
CATGACAACAGGTTGCATGG+TGG + chr8.4:60342107-60342126 MS.gene92397:CDS 50.0%
CCGGAATCGATCGAACTTGT+TGG - chr8.4:60342435-60342454 None:intergenic 50.0%
CCTCAAGTTCGACCCAATCT+GGG + chr8.4:60342397-60342416 MS.gene92397:CDS 50.0%
CGAACTTGAGGTCGAACTTG+AGG - chr8.4:60342388-60342407 None:intergenic 50.0%
CGAGAAATCTCCATTGCCTG+AGG + chr8.4:60342458-60342477 MS.gene92397:CDS 50.0%
CTCTGCTCTCAAGATTCCAC+CGG + chr8.4:60341886-60341905 MS.gene92397:CDS 50.0%
CTGCATGACAACAGGTTGCA+TGG + chr8.4:60342104-60342123 MS.gene92397:CDS 50.0%
GAATTCGTACCACGTACCGA+AGG - chr8.4:60342586-60342605 None:intergenic 50.0%
GCAAAGCCGGTTGTAGAGAA+CGG - chr8.4:60341796-60341815 None:intergenic 50.0%
GGTGGAATCTTGAGAGCAGA+GGG - chr8.4:60341887-60341906 None:intergenic 50.0%
GTGCTGTGATTGAACCACGT+TGG - chr8.4:60342811-60342830 None:intergenic 50.0%
GTTTCCTCCAGCACACTCTA+TGG - chr8.4:60341950-60341969 None:intergenic 50.0%
TAAGCGTAGCTATAAGCGCG+AGG - chr8.4:60342306-60342325 None:intergenic 50.0%
TCGGTACGTGGTACGAATTC+GGG + chr8.4:60342586-60342605 MS.gene92397:intron 50.0%
! AAGCGCATGTGAAGCGTTTG+GGG + chr8.4:60342194-60342213 MS.gene92397:CDS 50.0%
! CGGTACGCTATTTGGGTTCA+TGG + chr8.4:60342660-60342679 MS.gene92397:intron 50.0%
! GTGGTTCTATCGTCATTGGG+AGG - chr8.4:60342062-60342081 None:intergenic 50.0%
! TTCGACGTTAGTCTCGTGGA+CGG + chr8.4:60342030-60342049 MS.gene92397:CDS 50.0%
AACTCGGCTAATGTAGCCGG+TGG - chr8.4:60341979-60341998 None:intergenic 55.0%
AAGTGGTCAGGCCGTCTATG+GGG + chr8.4:60341856-60341875 MS.gene92397:CDS 55.0%
ACACGGTTCGCCATTCCCTT+CGG + chr8.4:60342567-60342586 MS.gene92397:intron 55.0%
AGCACAGTCACCGGTAACAC+AGG - chr8.4:60341920-60341939 None:intergenic 55.0%
CAAGTGGTCAGGCCGTCTAT+GGG + chr8.4:60341855-60341874 MS.gene92397:CDS 55.0%
CATAGACGGCCTGACCACTT+GGG - chr8.4:60341856-60341875 None:intergenic 55.0%
CCCAGATTGGGTCGAACTTG+AGG - chr8.4:60342400-60342419 None:intergenic 55.0%
CGGTGGAATCTTGAGAGCAG+AGG - chr8.4:60341888-60341907 None:intergenic 55.0%
GGCGGAAACTGCATGACAAC+AGG + chr8.4:60342096-60342115 MS.gene92397:CDS 55.0%
GTACCACGTACCGAAGGGAA+TGG - chr8.4:60342580-60342599 None:intergenic 55.0%
GTGGTACGAATTCGGGTACG+CGG + chr8.4:60342593-60342612 MS.gene92397:intron 55.0%
TCCACCATAGAGTGTGCTGG+AGG + chr8.4:60341943-60341962 MS.gene92397:CDS 55.0%
TCCAGCACACTCTATGGTGG+AGG - chr8.4:60341944-60341963 None:intergenic 55.0%
TCCTCCACCATAGAGTGTGC+TGG + chr8.4:60341940-60341959 MS.gene92397:CDS 55.0%
TCCTCCAGCACACTCTATGG+TGG - chr8.4:60341947-60341966 None:intergenic 55.0%
TGCAACTACACCGTTTGGCC+TGG + chr8.4:60341745-60341764 MS.gene92397:CDS 55.0%
TGGAGGAAACGCTCAACCAC+CGG + chr8.4:60341960-60341979 MS.gene92397:CDS 55.0%
TGTCATGCAGTTTCCGCCTC+CGG - chr8.4:60342094-60342113 None:intergenic 55.0%
! ACGTGGGGGGTATACAGAGT+CGG + chr8.4:60342640-60342659 MS.gene92397:intron 55.0%
! AGAGGGTACGACCCCATAGA+CGG - chr8.4:60341870-60341889 None:intergenic 55.0%
! CCTGGAATTTTATCCGGCGC+CGG + chr8.4:60341763-60341782 MS.gene92397:CDS 55.0%
CCAAGTGGTCAGGCCGTCTA+TGG + chr8.4:60341854-60341873 MS.gene92397:CDS 60.0%
CCATAGACGGCCTGACCACT+TGG - chr8.4:60341857-60341876 None:intergenic 60.0%
GAACCACATGGAGCAACCGG+AGG + chr8.4:60342075-60342094 MS.gene92397:CDS 60.0%
GCCTGACCACTTGGGAGGTA+CGG - chr8.4:60341848-60341867 None:intergenic 60.0%
TCGCCATTCCCTTCGGTACG+TGG + chr8.4:60342574-60342593 MS.gene92397:intron 60.0%
! ACCACTTGGGAGGTACGGCA+AGG - chr8.4:60341843-60341862 None:intergenic 60.0%
! ACCGGCTTTGCTCTCCAACC+CGG + chr8.4:60341805-60341824 MS.gene92397:CDS 60.0%
! CCACTTGGGAGGTACGGCAA+GGG - chr8.4:60341842-60341861 None:intergenic 60.0%
! CCGGCGCCGGATAAAATTCC+AGG - chr8.4:60341766-60341785 None:intergenic 60.0%
! GGTGGAGGAAGCACAGTCAC+CGG - chr8.4:60341929-60341948 None:intergenic 60.0%
!! GCAAGGGCATTGGATTCGCC+GGG - chr8.4:60341826-60341845 None:intergenic 60.0%
!! GGCAAGGGCATTGGATTCGC+CGG - chr8.4:60341827-60341846 None:intergenic 60.0%
AGACGGCCTGACCACTTGGG+AGG - chr8.4:60341853-60341872 None:intergenic 65.0%
CCACATGGAGCAACCGGAGG+CGG + chr8.4:60342078-60342097 MS.gene92397:CDS 65.0%
CCCTTGCCGTACCTCCCAAG+TGG + chr8.4:60341839-60341858 MS.gene92397:CDS 65.0%
GCCGTACCTCCCAAGTGGTC+AGG + chr8.4:60341844-60341863 MS.gene92397:CDS 65.0%
! GCCGGGTTGGAGAGCAAAGC+CGG - chr8.4:60341809-60341828 None:intergenic 65.0%
! GGGAGGTACGGCAAGGGCAT+TGG - chr8.4:60341836-60341855 None:intergenic 65.0%
!! GAACGGTGTTGTTCCGGCGC+CGG - chr8.4:60341779-60341798 None:intergenic 65.0%
!! GGGCATTGGATTCGCCGGGT+TGG - chr8.4:60341822-60341841 None:intergenic 65.0%
CCGCCTCCGGTTGCTCCATG+TGG - chr8.4:60342081-60342100 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 60341646 60343151 60341646 ID=MS.gene92397
chr8.4 mRNA 60341646 60343151 60341646 ID=MS.gene92397.t1;Parent=MS.gene92397
chr8.4 exon 60341646 60342524 60341646 ID=MS.gene92397.t1.exon1;Parent=MS.gene92397.t1
chr8.4 CDS 60341646 60342524 60341646 ID=cds.MS.gene92397.t1;Parent=MS.gene92397.t1
chr8.4 exon 60343065 60343151 60343065 ID=MS.gene92397.t1.exon2;Parent=MS.gene92397.t1
chr8.4 CDS 60343065 60343151 60343065 ID=cds.MS.gene92397.t1;Parent=MS.gene92397.t1
Gene Sequence

>MS.gene92397

ATGGCTGCTTCAACTCAATCAACCATCGCTTTGGTTACCCTAATTCTCTTCCAATACCTAACTGGTTCATATGCAACAACATTCACAATTGTTAACGAGTGCAACTACACCGTTTGGCCTGGAATTTTATCCGGCGCCGGAACAACACCGTTCTCTACAACCGGCTTTGCTCTCCAACCCGGCGAATCCAATGCCCTTGCCGTACCTCCCAAGTGGTCAGGCCGTCTATGGGGTCGTACCCTCTGCTCTCAAGATTCCACCGGAAAATTCTCCTGTGTTACCGGTGACTGTGCTTCCTCCACCATAGAGTGTGCTGGAGGAAACGCTCAACCACCGGCTACATTAGCCGAGTTTACACTTAACGGTGCAAACGGACTAGATTTCTTCGACGTTAGTCTCGTGGACGGTTACAACCTCCCAATGACGATAGAACCACATGGAGCAACCGGAGGCGGAAACTGCATGACAACAGGTTGCATGGTGGATTTGAACGCTGCGTGTCCAACCGAGTTGAAAGTGATGAGTAATGGCGGAGGTGTAGTGTGTAAAAGCGCATGTGAAGCGTTTGGGGATCCGCAATATTGTTGTAGTGGCGCTTACGCTTCTCCAGATACATGCAAGCCAAGTTCATACTCGCAGTTTTTCAAGAGTGCGTGTCCTCGCGCTTATAGCTACGCTTATGATGATGGAACAAGCACTTTTACTTGTGATTCTGCTGATTATTCCATCACTTTTTGTCCCTCAAGTTCGACCTCAAGTTCGACCCAATCTGGGAACGAGAAATCTCCAACAAGTTCGATCGATTCCGGGAACGAGAAATCTCCATTGCCTGAGGAGATGGATATATATGGGGGACATGTGAACTTCACAGCCAACAATGTTATGATTGCTGCTATTATTTCTTTTGTTTTGATCATAGTCACACGGTTCGCCATTCCCTTCGGTACGTGGTACGAATTCGGGTACGCGGTTCGTGACTTTCAAAAAACTAGGTACGTGGGGGGTATACAGAGTCGGTACGCTATTTGGGTTCATGGTACGTTCAAATATAATAATCGGTACGTTACTAATTAAAAAAATATTTAATTAACAAAAAATATAATAGAAAATGAAAACTATTTTTTTAGAGAGCAAATAAAAATTCATTCATTATATTAAAAGCCCAACGTGGTTCAATCACAGCACAATTGCAAACAGAATTGATATAACAACCAATAGAAACACACACCCATTGATCTATTCCATCCAAAAAATTCTGCTAAATTCTCTCAACAAAGAATATCAAGTCTTAAAAAAAAAGAGTTAAAAAAAAAGAGTTTTTCAAGAGTGCGTGTCCTCGCGCTTATAGCTACGCTTATGATGATGGAACAAGCACTTTTGTGATTCTGCTGATTATTCCATCACTTTTTGTCCCTCAAGTTCGATCTCAAGTTCGACCCAATCTGGGAACGAGAAATCTCCAACAAGTTCGATCGATTCCGGGAACGAGAAATCTCCATTGCCTGA
Protein sequence

>MS.gene92397.t1

MAASTQSTIALVTLILFQYLTGSYATTFTIVNECNYTVWPGILSGAGTTPFSTTGFALQPGESNALAVPPKWSGRLWGRTLCSQDSTGKFSCVTGDCASSTIECAGGNAQPPATLAEFTLNGANGLDFFDVSLVDGYNLPMTIEPHGATGGGNCMTTGCMVDLNAACPTELKVMSNGGGVVCKSACEAFGDPQYCCSGAYASPDTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCDSADYSITFCPSSSTSSSTQSGNEKSPTSSIDSGNEKSPLPEEMDIYGGHVNFTANNFDLKFDPIWEREISNKFDRFREREISIA