AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64778

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64778.t1 MTR_5g022310 93.770 305 14 2 1 305 1 300 0.0 576
MS.gene64778.t1 MTR_5g022310 95.703 256 9 1 1 256 1 254 7.05e-180 496
MS.gene64778.t1 MTR_8g037890 71.827 323 61 6 1 300 1 316 1.17e-157 442
MS.gene64778.t1 MTR_8g056820 80.077 261 46 1 1 261 1 255 1.68e-150 422
MS.gene64778.t1 MTR_8g075550 67.881 302 90 4 1 297 1 300 6.95e-138 392
MS.gene64778.t1 MTR_1g025420 67.717 254 75 4 14 266 11 258 1.36e-118 344
MS.gene64778.t1 MTR_1g025420 67.742 248 74 3 14 260 11 253 2.62e-117 338
MS.gene64778.t1 MTR_3g114030 70.417 240 65 3 20 258 1 235 4.41e-117 337
MS.gene64778.t1 MTR_4g073730 60.755 265 95 3 11 270 5 265 1.65e-107 315
MS.gene64778.t1 MTR_4g073730 60.755 265 95 3 11 270 5 265 3.66e-107 315
MS.gene64778.t1 MTR_4g073720 57.613 243 87 4 14 252 9 239 1.02e-90 274
MS.gene64778.t1 MTR_5g022350 53.903 269 106 7 1 266 4 257 2.88e-88 266
MS.gene64778.t1 MTR_8g075510 54.118 255 99 7 3 256 10 247 4.34e-84 253
MS.gene64778.t1 MTR_8g075510 54.183 251 97 7 3 252 10 243 1.06e-82 252
MS.gene64778.t1 MTR_7g102380 50.199 251 113 5 4 253 2 241 5.56e-82 248
MS.gene64778.t1 MTR_2g068030 51.304 230 102 5 25 252 22 243 8.58e-79 240
MS.gene64778.t1 MTR_2g069660 52.653 245 107 5 11 253 6 243 1.62e-78 239
MS.gene64778.t1 MTR_2g068655 50.644 233 107 3 22 253 27 252 2.48e-78 241
MS.gene64778.t1 MTR_8g036215 49.593 246 115 4 7 252 1 237 5.53e-78 238
MS.gene64778.t1 MTR_1g062590 49.200 250 116 5 7 253 1 242 7.83e-78 237
MS.gene64778.t1 MTR_2g067980 47.104 259 123 5 10 262 6 256 2.08e-75 249
MS.gene64778.t1 MTR_6g079580 48.996 249 112 5 11 253 1 240 2.63e-75 231
MS.gene64778.t1 MTR_1g062630 51.383 253 108 7 7 254 1 243 3.59e-75 231
MS.gene64778.t1 MTR_1g062660 50.410 244 108 6 12 254 12 243 6.68e-75 230
MS.gene64778.t1 MTR_8g107140 50.417 240 110 4 14 253 15 245 1.18e-74 229
MS.gene64778.t1 MTR_1g062340 49.794 243 109 6 13 254 13 243 2.63e-74 228
MS.gene64778.t1 MTR_3g111620 53.247 231 98 5 28 257 29 250 1.25e-73 227
MS.gene64778.t1 MTR_1g021945 51.915 235 101 6 25 257 25 249 2.30e-73 226
MS.gene64778.t1 MTR_1g062350 48.617 253 118 6 3 254 2 243 6.20e-73 225
MS.gene64778.t1 MTR_1g062370 47.826 253 120 6 3 254 2 243 1.78e-72 224
MS.gene64778.t1 MTR_5g023850 45.455 264 99 5 1 260 1 223 3.06e-69 214
MS.gene64778.t1 MTR_7g062610 44.664 253 130 4 5 256 2 245 3.65e-69 215
MS.gene64778.t1 MTR_5g059200 41.297 293 146 9 12 299 11 282 1.35e-68 215
MS.gene64778.t1 MTR_3g068015 45.652 230 114 5 26 253 28 248 1.53e-63 201
MS.gene64778.t1 MTR_6g009480 38.298 282 159 5 1 280 4 272 4.98e-62 198
MS.gene64778.t1 MTR_1g062690 43.083 253 108 7 3 254 2 219 3.97e-57 184
MS.gene64778.t1 MTR_4g063630 41.564 243 130 8 14 252 17 251 5.91e-56 182
MS.gene64778.t1 MTR_2g067990 42.562 242 105 7 22 262 23 231 1.37e-53 188
MS.gene64778.t1 MTR_5g010640 38.314 261 128 9 1 253 1 236 6.67e-47 158
MS.gene64778.t1 MTR_5g010635 39.914 233 110 8 23 253 21 225 1.46e-46 157
MS.gene64778.t1 MTR_2g067970 45.026 191 94 6 69 256 3 185 4.61e-46 166
MS.gene64778.t1 MTR_8g096900 38.645 251 119 9 9 253 27 248 8.40e-45 153
MS.gene64778.t1 MTR_5g022350 48.125 160 75 4 1 158 69 222 2.38e-39 138
MS.gene64778.t1 MTR_2g063150 34.545 220 131 5 27 245 35 242 2.83e-39 139
MS.gene64778.t1 MTR_8g089020 48.889 135 58 3 111 245 33 156 2.79e-36 128
MS.gene64778.t1 MTR_7g076360 35.616 219 71 4 25 243 7 155 4.28e-32 117
MS.gene64778.t1 MTR_1g062390 31.660 259 150 9 1 253 1 238 5.98e-31 116
MS.gene64778.t1 MTR_8g096910 43.165 139 61 6 10 141 2 129 1.20e-26 103
MS.gene64778.t1 MTR_8g096920 41.176 136 69 5 10 143 2 128 9.45e-22 90.5
MS.gene64778.t1 MTR_3g081550 48.544 103 48 2 107 209 12 109 9.54e-22 88.6
MS.gene64778.t1 MTR_1g062640 42.222 90 49 2 79 168 60 146 4.11e-16 74.7
MS.gene64778.t1 MTR_2g063160 36.170 94 56 3 30 121 18 109 1.94e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64778.t1 AT4G36010 59.028 288 107 4 22 304 19 300 7.49e-115 333
MS.gene64778.t1 AT4G36010 59.028 288 107 4 22 304 19 300 7.49e-115 333
MS.gene64778.t1 AT1G75800 63.672 256 88 4 11 264 7 259 8.77e-113 329
MS.gene64778.t1 AT2G17860 63.306 248 84 4 11 256 10 252 9.84e-110 319
MS.gene64778.t1 AT1G20030 66.667 240 72 4 22 258 16 250 3.39e-108 317
MS.gene64778.t1 AT1G20030 67.089 237 70 4 25 258 2 233 6.95e-108 316
MS.gene64778.t1 AT4G38670 64.050 242 80 4 27 266 24 260 9.18e-105 307
MS.gene64778.t1 AT4G38670 64.050 242 80 4 27 266 24 260 9.18e-105 307
MS.gene64778.t1 AT4G38670 64.050 242 80 4 27 266 8 244 3.00e-104 305
MS.gene64778.t1 AT4G38660 53.819 288 107 6 1 274 2 277 1.67e-96 288
MS.gene64778.t1 AT4G38660 56.180 267 94 5 19 274 1 255 7.20e-96 286
MS.gene64778.t1 AT4G24180 54.945 273 102 7 1 263 6 267 2.70e-92 276
MS.gene64778.t1 AT4G24180 55.513 263 97 6 1 254 6 257 4.66e-92 274
MS.gene64778.t1 AT4G24180 56.977 258 92 6 14 263 16 262 4.59e-91 273
MS.gene64778.t1 AT4G24180 55.556 261 95 6 1 254 6 252 9.01e-91 271
MS.gene64778.t1 AT5G24620 55.918 245 104 4 11 253 9 251 1.02e-86 266
MS.gene64778.t1 AT5G24620 55.918 245 104 4 11 253 85 327 2.26e-86 268
MS.gene64778.t1 AT5G24620 55.918 245 104 4 11 253 64 306 2.45e-86 267
MS.gene64778.t1 AT5G38280 51.181 254 119 3 7 258 5 255 2.46e-85 270
MS.gene64778.t1 AT5G38280 51.181 254 119 3 7 258 5 255 3.51e-85 269
MS.gene64778.t1 AT4G24180 53.441 247 95 6 1 238 6 241 1.71e-80 247
MS.gene64778.t1 AT1G75040 53.086 243 102 5 11 253 9 239 9.59e-80 242
MS.gene64778.t1 AT1G19320 50.813 246 107 5 11 252 11 246 2.39e-79 241
MS.gene64778.t1 AT4G24180 53.469 245 93 6 1 238 6 236 2.41e-79 244
MS.gene64778.t1 AT1G75030 52.743 237 102 7 21 256 19 246 1.85e-77 236
MS.gene64778.t1 AT1G75050 49.606 254 118 6 1 254 1 244 5.86e-77 235
MS.gene64778.t1 AT1G73620 52.402 229 100 4 25 253 41 260 3.72e-76 234
MS.gene64778.t1 AT1G18250 51.515 231 103 4 23 253 19 240 3.77e-75 231
MS.gene64778.t1 AT1G18250 51.528 229 102 4 25 253 20 239 4.48e-75 230
MS.gene64778.t1 AT1G77700 47.755 245 108 7 12 252 43 271 2.39e-70 221
MS.gene64778.t1 AT1G77700 47.347 245 109 7 12 252 43 271 3.48e-70 220
MS.gene64778.t1 AT1G77700 49.561 228 99 6 25 252 88 299 7.18e-70 221
MS.gene64778.t1 AT4G36000 66.460 161 38 3 102 262 1 145 5.37e-67 208
MS.gene64778.t1 AT4G36000 66.460 161 38 3 102 262 1 145 5.37e-67 208
MS.gene64778.t1 AT5G02140 41.071 280 146 5 9 277 2 273 3.00e-64 204
MS.gene64778.t1 AT5G02140 43.548 248 129 4 9 253 2 241 4.60e-64 202
MS.gene64778.t1 AT2G28790 42.913 254 132 7 3 252 4 248 5.15e-61 194
MS.gene64778.t1 AT2G28790 42.913 254 132 7 3 252 29 273 6.56e-61 195
MS.gene64778.t1 AT4G18253 42.629 251 127 5 11 258 7 243 2.27e-59 190
MS.gene64778.t1 AT5G40020 40.851 235 130 4 21 254 23 249 3.95e-59 190
MS.gene64778.t1 AT1G70250 44.118 238 118 7 25 257 144 371 1.19e-58 201
MS.gene64778.t1 AT4G18250 39.465 299 157 10 11 302 40 321 1.66e-54 188
MS.gene64778.t1 AT4G11650 37.729 273 134 10 7 275 1 241 3.64e-49 164
MS.gene64778.t1 AT5G24620 61.983 121 45 1 133 253 15 134 5.46e-44 152
MS.gene64778.t1 AT2G24810 56.627 83 30 1 170 252 116 192 4.19e-26 102

Find 97 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTGGTCTGGCCGTCTA+TGG 0.191862 5.2:+13364831 MS.gene64778:CDS
TTAGGAGTTGGTAGAGAATA+AGG 0.252939 5.2:-13364449 None:intergenic
TGGCGGGAGTGTTACCTATT+AGG 0.288934 5.2:-13364467 None:intergenic
TATTGCTGCAGTGGCGCTTA+TGG 0.293843 5.2:+13365220 MS.gene64778:CDS
GAATGCAGTTCAATCAAATC+TGG 0.324954 5.2:+13366013 MS.gene64778:intron
CTCTCGCCGTACCACCTTCC+TGG 0.337002 5.2:+13364816 MS.gene64778:CDS
ATTCTCTACCAACTCCTAAT+AGG 0.344279 5.2:+13364453 MS.gene64778:CDS
AAAGCGCGTGTGAAGCGTTT+GGG 0.355433 5.2:+13365188 MS.gene64778:CDS
CCGTGTGGTTCGATCAATAT+CGG 0.357683 5.2:-13365040 None:intergenic
ACAATTGCGGTTACACAGTT+TGG 0.365361 5.2:+13364717 MS.gene64778:CDS
GGCGGAAACTGCACGGCAAC+GGG 0.381206 5.2:+13365073 MS.gene64778:CDS
GAACTTGGTTTACACGTATC+AGG 0.390219 5.2:-13365247 None:intergenic
TATAGTTATGCTTATGATGA+TGG 0.395195 5.2:+13365307 MS.gene64778:CDS
GAATTCACCCTCAACGGTTC+CGG 0.396695 5.2:+13364971 MS.gene64778:CDS
CCGGCGCCGGATAAAATTCC+CGG 0.403457 5.2:-13364740 None:intergenic
ATCGAACCACACGGTGAAAC+CGG 0.404042 5.2:+13365049 MS.gene64778:CDS
CGGTGGTATAGCGTTTCTTC+CGG 0.406204 5.2:-13364936 None:intergenic
TTCCTGGTCTGGCCGTCTAT+GGG 0.409304 5.2:+13364832 MS.gene64778:CDS
TGCCGTGCAGTTTCCGCCGC+CGG 0.412387 5.2:-13365068 None:intergenic
GGTGGAGAATGGTTCTGTTC+CGG 0.429649 5.2:-13364759 None:intergenic
GTGAATTCTGCTAATGTTGC+CGG 0.433552 5.2:-13364956 None:intergenic
GCCGTACCACCTTCCTGGTC+TGG 0.449891 5.2:+13364821 MS.gene64778:CDS
GGCGAGAGCGTTGGACTCGC+CGG 0.450480 5.2:-13364801 None:intergenic
AAAAGCGCGTGTGAAGCGTT+TGG 0.451521 5.2:+13365187 MS.gene64778:CDS
AAATTACCTTGTGGAGGGTG+TGG 0.467285 5.2:-13365380 None:intergenic
CTTTGGTGGTTGAACGGTTG+TGG 0.468098 5.2:+13366140 MS.gene64778:CDS
AAGTCCTCCGGAACCGTTGA+GGG 0.477120 5.2:-13364978 None:intergenic
ACAAAGCCGGTGGTGGAGAA+TGG 0.482809 5.2:-13364770 None:intergenic
GGAAAATTCTCATGCATCAC+CGG 0.483024 5.2:+13364884 MS.gene64778:CDS
CGGCGGAAACTGCACGGCAA+CGG 0.484219 5.2:+13365072 MS.gene64778:CDS
TACTCAATTTGCCACCACTT+TGG 0.487012 5.2:+13364422 MS.gene64778:CDS
TAGGAGTTGGTAGAGAATAA+GGG 0.493076 5.2:-13364448 None:intergenic
GTGCCAGCTATGGACGTGTC+TGG 0.496509 5.2:+13366052 MS.gene64778:CDS
GAATGGTTCTGTTCCGGCGC+CGG 0.508113 5.2:-13364753 None:intergenic
TTGGAGAACAAAGCCGGTGG+TGG 0.508758 5.2:-13364777 None:intergenic
TTGCGGTTACACAGTTTGGC+CGG 0.509361 5.2:+13364721 MS.gene64778:CDS
ACACCAGACACGTCCATAGC+TGG 0.514018 5.2:-13366055 None:intergenic
TGCGGTTACACAGTTTGGCC+GGG 0.517234 5.2:+13364722 MS.gene64778:CDS
GCGAGAGCGTTGGACTCGCC+GGG 0.518695 5.2:-13364800 None:intergenic
GTTTCTTCCGGCACATTCGA+TGG 0.519305 5.2:-13364924 None:intergenic
AAAATATCATGTGCCAGCTA+TGG 0.521922 5.2:+13366042 MS.gene64778:CDS
TTCACAATTGTGAACAATTG+CGG 0.527708 5.2:+13364704 MS.gene64778:CDS
TCCCCATAGACGGCCAGACC+AGG 0.532075 5.2:-13364834 None:intergenic
CCGCCGCCGGTTTCACCGTG+TGG 0.538076 5.2:-13365055 None:intergenic
TGACGGCTTTGGTGGTTGAA+CGG 0.539340 5.2:+13366134 MS.gene64778:CDS
AATTACCTTGTGGAGGGTGT+GGG 0.540652 5.2:-13365379 None:intergenic
CGTGTGGTTCGATCAATATC+GGG 0.545709 5.2:-13365039 None:intergenic
CTTGAACGCGGCGTGTCCGA+CGG 0.547717 5.2:+13365108 MS.gene64778:CDS
GAGCGTTGGACTCGCCGGGT+TGG 0.550394 5.2:-13364796 None:intergenic
TCCGGCACATTCGATGGTGG+AGG 0.550974 5.2:-13364918 None:intergenic
AATGCAGTTCAATCAAATCT+GGG 0.551133 5.2:+13366014 MS.gene64778:intron
TCCTCCACCATCGAATGTGC+CGG 0.552071 5.2:+13364917 MS.gene64778:CDS
AAGCGCGTGTGAAGCGTTTG+GGG 0.554522 5.2:+13365189 MS.gene64778:CDS
GATCCGCAATATTGCTGCAG+TGG 0.557457 5.2:+13365211 MS.gene64778:CDS
GTCGAAGAAATCAAGTCCTC+CGG 0.560323 5.2:-13364990 None:intergenic
GCCGGGTTGGAGAACAAAGC+CGG 0.572484 5.2:-13364783 None:intergenic
ACAGAACCATTCTCCACCAC+CGG 0.573244 5.2:+13364764 MS.gene64778:CDS
AGGTGGTACGGCGAGAGCGT+TGG 0.574498 5.2:-13364810 None:intergenic
GCCAGACCAGGAAGGTGGTA+CGG 0.576483 5.2:-13364822 None:intergenic
AGTAGTAGTAATAGCGGCGG+AGG 0.580917 5.2:+13365145 MS.gene64778:CDS
AAGTGAAAATTACCTTGTGG+AGG 0.581305 5.2:-13365386 None:intergenic
ACCGGCTTTGTTCTCCAACC+CGG 0.581948 5.2:+13364782 MS.gene64778:CDS
CGTGTCTGGTGTTCGACATG+TGG 0.582894 5.2:+13366066 MS.gene64778:CDS
CTCATCACTTTCAACTCCGT+CGG 0.583041 5.2:-13365124 None:intergenic
GAGTGTTACCTATTAGGAGT+TGG 0.590111 5.2:-13364461 None:intergenic
AGTGTGTTCTTCCCCATAGA+CGG 0.590774 5.2:-13364844 None:intergenic
ATTACCTTGTGGAGGGTGTG+GGG 0.591516 5.2:-13365378 None:intergenic
AACCGGCGGCGGAAACTGCA+CGG 0.591968 5.2:+13365066 MS.gene64778:CDS
CACCAAAGCCGTCAAAACAA+AGG 0.604676 5.2:-13366125 None:intergenic
TCCTGGTCTGGCCGTCTATG+GGG 0.605231 5.2:+13364833 MS.gene64778:CDS
CCACACGGTGAAACCGGCGG+CGG 0.608059 5.2:+13365055 MS.gene64778:CDS
CACTGCTCTCAAGACCCCAC+CGG 0.608797 5.2:+13364863 MS.gene64778:CDS
TTAGCAGAATTCACCCTCAA+CGG 0.609111 5.2:+13364965 MS.gene64778:CDS
GCGCCACTGCAGCAATATTG+CGG 0.611330 5.2:-13365214 None:intergenic
AGTGAAAATTACCTTGTGGA+GGG 0.612016 5.2:-13365385 None:intergenic
GAGAATAAGGGCAACCAAAG+TGG 0.615615 5.2:-13364436 None:intergenic
CAAGTCCTCCGGAACCGTTG+AGG 0.616426 5.2:-13364979 None:intergenic
TTCACCCTCAACGGTTCCGG+AGG 0.618797 5.2:+13364974 MS.gene64778:CDS
TTCGACGTTAGTCTCGTCGA+CGG 0.621410 5.2:+13365007 MS.gene64778:CDS
CGGCGGAGGTGAAGAGAGCG+TGG 0.621463 5.2:+13365159 MS.gene64778:CDS
ACACGCCGCGTTCAAGTCGA+CGG 0.623825 5.2:-13365101 None:intergenic
CGGAAGAAACGCTATACCAC+CGG 0.628933 5.2:+13364937 MS.gene64778:CDS
TCTTCCGGCACATTCGATGG+TGG 0.630194 5.2:-13364921 None:intergenic
TTTGCCCCACACCCTCCACA+AGG 0.630850 5.2:+13365374 MS.gene64778:CDS
GGTGGAGGAATCACAGTCGC+CGG 0.634571 5.2:-13364903 None:intergenic
AGTTCAATCAAATCTGGGAA+CGG 0.635581 5.2:+13366019 MS.gene64778:intron
CATAGACGGCCAGACCAGGA+AGG 0.638136 5.2:-13364830 None:intergenic
ATGAGTAGTAGTAATAGCGG+CGG 0.652951 5.2:+13365142 MS.gene64778:CDS
GAACCACACGGTGAAACCGG+CGG 0.655360 5.2:+13365052 MS.gene64778:CDS
AATAAGGGCAACCAAAGTGG+TGG 0.656806 5.2:-13364433 None:intergenic
TTGCTCCGTCGACTTGAACG+CGG 0.663114 5.2:+13365096 MS.gene64778:CDS
CCGATATTGATCGAACCACA+CGG 0.663725 5.2:+13365040 MS.gene64778:CDS
AGACGGCCAGACCAGGAAGG+TGG 0.664276 5.2:-13364827 None:intergenic
GTGATGAGTAGTAGTAATAG+CGG 0.671487 5.2:+13365139 MS.gene64778:CDS
TAAGCATAACTATAAGCGCG+TGG 0.672663 5.2:-13365298 None:intergenic
AATTCTGCTAATGTTGCCGG+TGG 0.685668 5.2:-13364953 None:intergenic
GGGTTGGAGAACAAAGCCGG+TGG 0.695061 5.2:-13364780 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAATTAAATTAAAAAA+AGG - chr5.2:13365701-13365720 None:intergenic 0.0%
!! TCATAACAAAAAAAAAAATC+TGG - chr5.2:13365861-13365880 None:intergenic 15.0%
!! TTTAACATGTTAATTATTAC+AGG + chr5.2:13364665-13364684 MS.gene64778:intron 15.0%
!!! ATCTTTTACTAAAAAATATC+TGG - chr5.2:13365494-13365513 None:intergenic 15.0%
!!! CTTTTCAAAATGAATGTAAT+TGG + chr5.2:13365456-13365475 MS.gene64778:intron 20.0%
! AAAAAGTGAAAATTACCTTG+TGG - chr5.2:13365392-13365411 None:intergenic 25.0%
! ACAAAAAAAAAAATCTGGCA+TGG - chr5.2:13365856-13365875 None:intergenic 25.0%
! GTGATAAGTTATTGAAGAAA+AGG - chr5.2:13365425-13365444 None:intergenic 25.0%
! TATAGTTATGCTTATGATGA+TGG + chr5.2:13365307-13365326 MS.gene64778:CDS 25.0%
! TATCACCTTAGAATTAGATA+TGG + chr5.2:13365772-13365791 MS.gene64778:intron 25.0%
! TTTGTGAATCAACTAATGAT+TGG + chr5.2:13365609-13365628 MS.gene64778:intron 25.0%
AAAAAAAAATCTGGCATGGA+CGG - chr5.2:13365852-13365871 None:intergenic 30.0%
AAAAAACTAAACTGCATGTC+TGG - chr5.2:13364588-13364607 None:intergenic 30.0%
AATGCAGTTCAATCAAATCT+GGG + chr5.2:13366014-13366033 MS.gene64778:intron 30.0%
ACTACGAAAATCGAATAACT+TGG - chr5.2:13364490-13364509 None:intergenic 30.0%
ATAGAGAAAGTTGAATGTCA+AGG + chr5.2:13365722-13365741 MS.gene64778:intron 30.0%
CACAAAAACTAACTCATACA+CGG - chr5.2:13365920-13365939 None:intergenic 30.0%
GACAACCATATCTAATTCTA+AGG - chr5.2:13365780-13365799 None:intergenic 30.0%
TTCACAATTGTGAACAATTG+CGG + chr5.2:13364704-13364723 MS.gene64778:CDS 30.0%
!! TACACGGAATGTTTTATTGA+TGG - chr5.2:13365904-13365923 None:intergenic 30.0%
!! TGTTTTATTGATGGTACATC+TGG - chr5.2:13365895-13365914 None:intergenic 30.0%
!!! GTTAAAACGTGTTTTGAGAT+AGG + chr5.2:13365649-13365668 MS.gene64778:intron 30.0%
!!! TGTTATTGCCTTTGTTTTGA+CGG + chr5.2:13366117-13366136 MS.gene64778:CDS 30.0%
AAAATATCATGTGCCAGCTA+TGG + chr5.2:13366042-13366061 MS.gene64778:CDS 35.0%
AAGTGAAAATTACCTTGTGG+AGG - chr5.2:13365389-13365408 None:intergenic 35.0%
ACGAAAATCGAATAACTTGG+CGG - chr5.2:13364487-13364506 None:intergenic 35.0%
ACTCTTAAAAAACTGCGAGT+AGG - chr5.2:13365272-13365291 None:intergenic 35.0%
AGAAAGTTGAATGTCAAGGA+GGG + chr5.2:13365726-13365745 MS.gene64778:intron 35.0%
AGTGAAAATTACCTTGTGGA+GGG - chr5.2:13365388-13365407 None:intergenic 35.0%
AGTTCAATCAAATCTGGGAA+CGG + chr5.2:13366019-13366038 MS.gene64778:intron 35.0%
ATTCTCTACCAACTCCTAAT+AGG + chr5.2:13364453-13364472 MS.gene64778:CDS 35.0%
GAATGCAGTTCAATCAAATC+TGG + chr5.2:13366013-13366032 MS.gene64778:intron 35.0%
GTGATGAGTAGTAGTAATAG+CGG + chr5.2:13365139-13365158 MS.gene64778:CDS 35.0%
!! TAGGAGTTGGTAGAGAATAA+GGG - chr5.2:13364451-13364470 None:intergenic 35.0%
!! TTAGGAGTTGGTAGAGAATA+AGG - chr5.2:13364452-13364471 None:intergenic 35.0%
AAAAACTGCGAGTAGGAACT+TGG - chr5.2:13365265-13365284 None:intergenic 40.0%
ACAATTGCGGTTACACAGTT+TGG + chr5.2:13364717-13364736 MS.gene64778:CDS 40.0%
ATACCACACCATAACAGCAA+CGG - chr5.2:13365755-13365774 None:intergenic 40.0%
ATGAGTAGTAGTAATAGCGG+CGG + chr5.2:13365142-13365161 MS.gene64778:CDS 40.0%
CGAAAATCGAATAACTTGGC+GGG - chr5.2:13364486-13364505 None:intergenic 40.0%
GAAAGTTGAATGTCAAGGAG+GGG + chr5.2:13365727-13365746 MS.gene64778:intron 40.0%
GAACTTGGTTTACACGTATC+AGG - chr5.2:13365250-13365269 None:intergenic 40.0%
GAGAAAGTTGAATGTCAAGG+AGG + chr5.2:13365725-13365744 MS.gene64778:intron 40.0%
GAGTGTTACCTATTAGGAGT+TGG - chr5.2:13364464-13364483 None:intergenic 40.0%
GGAAAATTCTCATGCATCAC+CGG + chr5.2:13364884-13364903 MS.gene64778:CDS 40.0%
GTGAATTCTGCTAATGTTGC+CGG - chr5.2:13364959-13364978 None:intergenic 40.0%
TAAGCATAACTATAAGCGCG+TGG - chr5.2:13365301-13365320 None:intergenic 40.0%
TACTCAATTTGCCACCACTT+TGG + chr5.2:13364422-13364441 MS.gene64778:CDS 40.0%
TGCATGATCTAGCGTGATTA+TGG + chr5.2:13364545-13364564 MS.gene64778:intron 40.0%
TTAGCAGAATTCACCCTCAA+CGG + chr5.2:13364965-13364984 MS.gene64778:CDS 40.0%
! ATGCATGAGAATTTTCCGGT+GGG - chr5.2:13364881-13364900 None:intergenic 40.0%
! GGTGATGCATGAGAATTTTC+CGG - chr5.2:13364885-13364904 None:intergenic 40.0%
! TATTGATGGTACATCTGGCA+TGG - chr5.2:13365890-13365909 None:intergenic 40.0%
AAATTACCTTGTGGAGGGTG+TGG - chr5.2:13365383-13365402 None:intergenic 45.0%
AATAAGGGCAACCAAAGTGG+TGG - chr5.2:13364436-13364455 None:intergenic 45.0%
AATTCTGCTAATGTTGCCGG+TGG - chr5.2:13364956-13364975 None:intergenic 45.0%
AGTGTGTTCTTCCCCATAGA+CGG - chr5.2:13364847-13364866 None:intergenic 45.0%
CACCAAAGCCGTCAAAACAA+AGG - chr5.2:13366128-13366147 None:intergenic 45.0%
CCGATATTGATCGAACCACA+CGG + chr5.2:13365040-13365059 MS.gene64778:CDS 45.0%
CCGTGTGGTTCGATCAATAT+CGG - chr5.2:13365043-13365062 None:intergenic 45.0%
CGTGTGGTTCGATCAATATC+GGG - chr5.2:13365042-13365061 None:intergenic 45.0%
CTCATCACTTTCAACTCCGT+CGG - chr5.2:13365127-13365146 None:intergenic 45.0%
GAGAATAAGGGCAACCAAAG+TGG - chr5.2:13364439-13364458 None:intergenic 45.0%
GTCGAAGAAATCAAGTCCTC+CGG - chr5.2:13364993-13365012 None:intergenic 45.0%
TTGTGTAGCTAGCTACTAGC+TGG + chr5.2:13365935-13365954 MS.gene64778:intron 45.0%
! AATTACCTTGTGGAGGGTGT+GGG - chr5.2:13365382-13365401 None:intergenic 45.0%
! GATGCATGAGAATTTTCCGG+TGG - chr5.2:13364882-13364901 None:intergenic 45.0%
! GTTTGGCCGGGAATTTTATC+CGG + chr5.2:13364734-13364753 MS.gene64778:CDS 45.0%
! TGCATGAGAATTTTCCGGTG+GGG - chr5.2:13364880-13364899 None:intergenic 45.0%
!!! CTTTGTTTTGACGGCTTTGG+TGG + chr5.2:13366126-13366145 MS.gene64778:CDS 45.0%
!!! TGCCTTTGTTTTGACGGCTT+TGG + chr5.2:13366123-13366142 MS.gene64778:CDS 45.0%
ACAGAACCATTCTCCACCAC+CGG + chr5.2:13364764-13364783 MS.gene64778:CDS 50.0%
AGTAGTAGTAATAGCGGCGG+AGG + chr5.2:13365145-13365164 MS.gene64778:CDS 50.0%
ATCGAACCACACGGTGAAAC+CGG + chr5.2:13365049-13365068 MS.gene64778:CDS 50.0%
CGGAAGAAACGCTATACCAC+CGG + chr5.2:13364937-13364956 MS.gene64778:CDS 50.0%
CGGTGGTATAGCGTTTCTTC+CGG - chr5.2:13364939-13364958 None:intergenic 50.0%
GAATTCACCCTCAACGGTTC+CGG + chr5.2:13364971-13364990 MS.gene64778:CDS 50.0%
GATCCGCAATATTGCTGCAG+TGG + chr5.2:13365211-13365230 MS.gene64778:CDS 50.0%
GTTTCTTCCGGCACATTCGA+TGG - chr5.2:13364927-13364946 None:intergenic 50.0%
TATTGCTGCAGTGGCGCTTA+TGG + chr5.2:13365220-13365239 MS.gene64778:CDS 50.0%
TTGCGGTTACACAGTTTGGC+CGG + chr5.2:13364721-13364740 MS.gene64778:CDS 50.0%
! AAAAGCGCGTGTGAAGCGTT+TGG + chr5.2:13365187-13365206 MS.gene64778:CDS 50.0%
! AAAGCGCGTGTGAAGCGTTT+GGG + chr5.2:13365188-13365207 MS.gene64778:CDS 50.0%
! ATTACCTTGTGGAGGGTGTG+GGG - chr5.2:13365381-13365400 None:intergenic 50.0%
! CTTTGGTGGTTGAACGGTTG+TGG + chr5.2:13366140-13366159 MS.gene64778:CDS 50.0%
! GAACCGTTGCTGTTATGGTG+TGG + chr5.2:13365749-13365768 MS.gene64778:intron 50.0%
! GGTGGAGAATGGTTCTGTTC+CGG - chr5.2:13364762-13364781 None:intergenic 50.0%
! TGACGGCTTTGGTGGTTGAA+CGG + chr5.2:13366134-13366153 MS.gene64778:CDS 50.0%
! TTCGACGTTAGTCTCGTCGA+CGG + chr5.2:13365007-13365026 MS.gene64778:CDS 50.0%
!! TGGCGGGAGTGTTACCTATT+AGG - chr5.2:13364470-13364489 None:intergenic 50.0%
AAGTCCTCCGGAACCGTTGA+GGG - chr5.2:13364981-13365000 None:intergenic 55.0%
ACACCAGACACGTCCATAGC+TGG - chr5.2:13366058-13366077 None:intergenic 55.0%
CGTGTCTGGTGTTCGACATG+TGG + chr5.2:13366066-13366085 MS.gene64778:CDS 55.0%
GCGCCACTGCAGCAATATTG+CGG - chr5.2:13365217-13365236 None:intergenic 55.0%
GGGTGTGGGGCAAAAAGTGA+TGG - chr5.2:13365368-13365387 None:intergenic 55.0%
TCCTCCACCATCGAATGTGC+CGG + chr5.2:13364917-13364936 MS.gene64778:CDS 55.0%
TCTTCCGGCACATTCGATGG+TGG - chr5.2:13364924-13364943 None:intergenic 55.0%
TGCGGTTACACAGTTTGGCC+GGG + chr5.2:13364722-13364741 MS.gene64778:CDS 55.0%
TTCCTGGTCTGGCCGTCTAT+GGG + chr5.2:13364832-13364851 MS.gene64778:CDS 55.0%
TTGGAGAACAAAGCCGGTGG+TGG - chr5.2:13364780-13364799 None:intergenic 55.0%
! AAGCGCGTGTGAAGCGTTTG+GGG + chr5.2:13365189-13365208 MS.gene64778:CDS 55.0%
! GAGGGGAACCGTTGCTGTTA+TGG + chr5.2:13365744-13365763 MS.gene64778:intron 55.0%
!! ACAAAGCCGGTGGTGGAGAA+TGG - chr5.2:13364773-13364792 None:intergenic 55.0%
!! ACCGGCTTTGTTCTCCAACC+CGG + chr5.2:13364782-13364801 MS.gene64778:CDS 55.0%
!! TTGCTCCGTCGACTTGAACG+CGG + chr5.2:13365096-13365115 MS.gene64778:CDS 55.0%
ACACGCCGCGTTCAAGTCGA+CGG - chr5.2:13365104-13365123 None:intergenic 60.0%
CAAGTCCTCCGGAACCGTTG+AGG - chr5.2:13364982-13365001 None:intergenic 60.0%
CACTGCTCTCAAGACCCCAC+CGG + chr5.2:13364863-13364882 MS.gene64778:CDS 60.0%
CATAGACGGCCAGACCAGGA+AGG - chr5.2:13364833-13364852 None:intergenic 60.0%
CTTCCTGGTCTGGCCGTCTA+TGG + chr5.2:13364831-13364850 MS.gene64778:CDS 60.0%
GAACCACACGGTGAAACCGG+CGG + chr5.2:13365052-13365071 MS.gene64778:CDS 60.0%
GCCAGACCAGGAAGGTGGTA+CGG - chr5.2:13364825-13364844 None:intergenic 60.0%
GGGTTGGAGAACAAAGCCGG+TGG - chr5.2:13364783-13364802 None:intergenic 60.0%
GGTGGAGGAATCACAGTCGC+CGG - chr5.2:13364906-13364925 None:intergenic 60.0%
GTGCCAGCTATGGACGTGTC+TGG + chr5.2:13366052-13366071 MS.gene64778:CDS 60.0%
TCCTGGTCTGGCCGTCTATG+GGG + chr5.2:13364833-13364852 MS.gene64778:CDS 60.0%
TTCACCCTCAACGGTTCCGG+AGG + chr5.2:13364974-13364993 MS.gene64778:CDS 60.0%
TTTGCCCCACACCCTCCACA+AGG + chr5.2:13365374-13365393 MS.gene64778:CDS 60.0%
! CCGGCGCCGGATAAAATTCC+CGG - chr5.2:13364743-13364762 None:intergenic 60.0%
! CCGGGAATTTTATCCGGCGC+CGG + chr5.2:13364740-13364759 MS.gene64778:CDS 60.0%
! GAATGGTTCTGTTCCGGCGC+CGG - chr5.2:13364756-13364775 None:intergenic 60.0%
! GCCGGGTTGGAGAACAAAGC+CGG - chr5.2:13364786-13364805 None:intergenic 60.0%
! TCCGGCACATTCGATGGTGG+AGG - chr5.2:13364921-13364940 None:intergenic 60.0%
AACCGGCGGCGGAAACTGCA+CGG + chr5.2:13365066-13365085 MS.gene64778:CDS 65.0%
AGACGGCCAGACCAGGAAGG+TGG - chr5.2:13364830-13364849 None:intergenic 65.0%
CGGCGGAAACTGCACGGCAA+CGG + chr5.2:13365072-13365091 MS.gene64778:CDS 65.0%
CTCTCGCCGTACCACCTTCC+TGG + chr5.2:13364816-13364835 MS.gene64778:CDS 65.0%
CTTGAACGCGGCGTGTCCGA+CGG + chr5.2:13365108-13365127 MS.gene64778:CDS 65.0%
GCCGTACCACCTTCCTGGTC+TGG + chr5.2:13364821-13364840 MS.gene64778:CDS 65.0%
GGCGGAAACTGCACGGCAAC+GGG + chr5.2:13365073-13365092 MS.gene64778:CDS 65.0%
TCCCCATAGACGGCCAGACC+AGG - chr5.2:13364837-13364856 None:intergenic 65.0%
! AGGTGGTACGGCGAGAGCGT+TGG - chr5.2:13364813-13364832 None:intergenic 65.0%
CCACACGGTGAAACCGGCGG+CGG + chr5.2:13365055-13365074 MS.gene64778:CDS 70.0%
CGGCGGAGGTGAAGAGAGCG+TGG + chr5.2:13365159-13365178 MS.gene64778:CDS 70.0%
TGCCGTGCAGTTTCCGCCGC+CGG - chr5.2:13365071-13365090 None:intergenic 70.0%
!! GAGCGTTGGACTCGCCGGGT+TGG - chr5.2:13364799-13364818 None:intergenic 70.0%
!! GCGAGAGCGTTGGACTCGCC+GGG - chr5.2:13364803-13364822 None:intergenic 70.0%
!! GGCGAGAGCGTTGGACTCGC+CGG - chr5.2:13364804-13364823 None:intergenic 70.0%
!! CCGCCGCCGGTTTCACCGTG+TGG - chr5.2:13365058-13365077 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 13364411 13366165 13364411 ID=MS.gene64778
chr5.2 mRNA 13364411 13366165 13364411 ID=MS.gene64778.t1;Parent=MS.gene64778
chr5.2 exon 13364411 13364474 13364411 ID=MS.gene64778.t1.exon1;Parent=MS.gene64778.t1
chr5.2 CDS 13364411 13364474 13364411 ID=cds.MS.gene64778.t1;Parent=MS.gene64778.t1
chr5.2 exon 13364687 13365395 13364687 ID=MS.gene64778.t1.exon2;Parent=MS.gene64778.t1
chr5.2 CDS 13364687 13365395 13364687 ID=cds.MS.gene64778.t1;Parent=MS.gene64778.t1
chr5.2 exon 13366021 13366165 13366021 ID=MS.gene64778.t1.exon3;Parent=MS.gene64778.t1
chr5.2 CDS 13366021 13366165 13366021 ID=cds.MS.gene64778.t1;Parent=MS.gene64778.t1
Gene Sequence

>MS.gene64778

ATGGCTTCTTCTACTCAATTTGCCACCACTTTGGTTGCCCTTATTCTCTACCAACTCCTAATAGGTAACACTCCCGCCAAGTTATTCGATTTTCGTAGTGCATGTTTGAATTCACGATGAATTTGTTAGAATCATGCATGATCTAGCGTGATTATGGCAAATCTAACAATGATGCCAGACATGCAGTTTAGTTTTTTTTGTTGTTTGTTTTTAATGTTCACATAAAAGTTTGTGTCTTAAGTTGCTTTCTTGTTTTTAACATGTTAATTATTACAGGTTCATATTCATCAACATTCACAATTGTGAACAATTGCGGTTACACAGTTTGGCCGGGAATTTTATCCGGCGCCGGAACAGAACCATTCTCCACCACCGGCTTTGTTCTCCAACCCGGCGAGTCCAACGCTCTCGCCGTACCACCTTCCTGGTCTGGCCGTCTATGGGGAAGAACACACTGCTCTCAAGACCCCACCGGAAAATTCTCATGCATCACCGGCGACTGTGATTCCTCCACCATCGAATGTGCCGGAAGAAACGCTATACCACCGGCAACATTAGCAGAATTCACCCTCAACGGTTCCGGAGGACTTGATTTCTTCGACGTTAGTCTCGTCGACGGTTACAATCTCCCGATATTGATCGAACCACACGGTGAAACCGGCGGCGGAAACTGCACGGCAACGGGTTGCTCCGTCGACTTGAACGCGGCGTGTCCGACGGAGTTGAAAGTGATGAGTAGTAGTAATAGCGGCGGAGGTGAAGAGAGCGTGGCGTGTAAAAGCGCGTGTGAAGCGTTTGGGGATCCGCAATATTGCTGCAGTGGCGCTTATGGTTCGCCTGATACGTGTAAACCAAGTTCCTACTCGCAGTTTTTTAAGAGTGCGTGTCCACGCGCTTATAGTTATGCTTATGATGATGGAACTAGCACTTTCACTTGTGCTTCTGCTGATTACACCATCACTTTTTGCCCCACACCCTCCACAAGGTAATTTTCACTTTTTTTACTGTTTACCTTTTCTTCAATAACTTATCACAATTTTCTTTTCTTTTCAAAATGAATGTAATTGGTTGTCAAAAAGACCAGATATTTTTTAGTAAAAGATGCAAAGATATTTGATATGTTAGAACACAAGATTCGAACATTTGATTTTAGTGTGAATGAATTTTGTCGCTAAATATTTTAGAGTAAACAAAATAATTTGTGAATCAACTAATGATTGGAATTGCTATTATTAGTTGTTAAAACGTGTTTTGAGATAGGAATATTGCTATTGAATTTTCATATCACCTTTTTTTAATTTAATTATTAAGATAGAGAAAGTTGAATGTCAAGGAGGGGAACCGTTGCTGTTATGGTGTGGTATCACCTTAGAATTAGATATGGTTGTCACGTTGCATTTTTTCTTTGTTTTTATTTATTTATTTATTTATATATGCACCGTCCATGCCAGATTTTTTTTTTTGTTATGAACTATGCCATGCCAGATGTACCATCAATAAAACATTCCGTGTATGAGTTAGTTTTTGTGTAGCTAGCTACTAGCTGGTTTTGTTTTGTGTTGAAGTTTGAGTGCAACATTTTGAAAACTCTTGTTTGAATGTGAATGCAGTTCAATCAAATCTGGGAACGGAAAATATCATGTGCCAGCTATGGACGTGTCTGGTGTTCGACATGTGGATCAGAGAGTCAACAACGTTGTAACGATTGTTATTGCCTTTGTTTTGACGGCTTTGGTGGTTGAACGGTTGTGGTAA
Protein sequence

>MS.gene64778.t1

MASSTQFATTLVALILYQLLIGSYSSTFTIVNNCGYTVWPGILSGAGTEPFSTTGFVLQPGESNALAVPPSWSGRLWGRTHCSQDPTGKFSCITGDCDSSTIECAGRNAIPPATLAEFTLNGSGGLDFFDVSLVDGYNLPILIEPHGETGGGNCTATGCSVDLNAACPTELKVMSSSNSGGGEESVACKSACEAFGDPQYCCSGAYGSPDTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCASADYTITFCPTPSTSSIKSGNGKYHVPAMDVSGVRHVDQRVNNVVTIVIAFVLTALVVERLW