AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45764

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45764.t1 MTR_8g037890 89.836 305 19 1 1 293 1 305 0.0 548
MS.gene45764.t1 MTR_8g056820 86.400 250 34 0 1 250 1 250 7.91e-159 443
MS.gene45764.t1 MTR_5g022310 76.014 296 58 3 1 292 1 287 2.84e-156 438
MS.gene45764.t1 MTR_5g022310 81.641 256 43 2 1 252 1 256 2.50e-149 419
MS.gene45764.t1 MTR_8g075550 65.101 298 82 5 1 289 1 285 3.69e-126 362
MS.gene45764.t1 MTR_1g025420 64.639 263 86 5 14 271 11 271 3.98e-117 340
MS.gene45764.t1 MTR_1g025420 67.078 243 74 4 14 251 11 252 4.77e-115 333
MS.gene45764.t1 MTR_3g114030 69.528 233 67 3 20 249 1 232 9.10e-115 331
MS.gene45764.t1 MTR_4g073730 63.673 245 80 3 11 247 5 248 4.50e-112 326
MS.gene45764.t1 MTR_4g073730 63.673 245 80 3 11 247 5 248 1.19e-111 326
MS.gene45764.t1 MTR_4g073720 53.584 293 122 8 9 298 9 290 1.97e-94 283
MS.gene45764.t1 MTR_5g022350 52.727 275 116 7 6 276 12 276 1.74e-89 270
MS.gene45764.t1 MTR_8g075510 54.839 248 101 6 4 250 10 247 4.71e-85 256
MS.gene45764.t1 MTR_8g075510 53.030 264 111 7 4 264 10 263 5.98e-83 253
MS.gene45764.t1 MTR_7g102380 50.617 243 114 5 9 249 5 243 1.03e-81 247
MS.gene45764.t1 MTR_2g069660 53.680 231 103 3 19 247 15 243 7.64e-80 242
MS.gene45764.t1 MTR_2g068030 52.489 221 101 3 28 246 25 243 1.00e-79 242
MS.gene45764.t1 MTR_2g067980 54.113 231 100 3 24 250 22 250 1.38e-79 260
MS.gene45764.t1 MTR_2g068655 50.215 233 114 2 22 253 27 258 1.82e-78 241
MS.gene45764.t1 MTR_8g036215 49.791 239 115 4 9 246 3 237 7.14e-78 237
MS.gene45764.t1 MTR_1g021945 53.947 228 99 4 25 250 25 248 1.51e-76 234
MS.gene45764.t1 MTR_1g062350 50.806 248 114 6 4 249 3 244 1.60e-76 234
MS.gene45764.t1 MTR_1g062660 51.464 239 109 5 13 249 11 244 3.50e-76 233
MS.gene45764.t1 MTR_1g062370 50.000 248 116 6 4 249 3 244 3.53e-76 233
MS.gene45764.t1 MTR_1g062590 48.583 247 119 5 3 247 2 242 1.50e-75 231
MS.gene45764.t1 MTR_1g062340 49.791 239 113 5 13 249 11 244 5.01e-75 230
MS.gene45764.t1 MTR_1g062630 49.402 251 119 6 3 251 2 246 4.63e-74 228
MS.gene45764.t1 MTR_6g079580 49.388 245 111 5 11 247 1 240 8.57e-74 227
MS.gene45764.t1 MTR_3g111620 52.193 228 103 5 25 250 26 249 2.87e-73 226
MS.gene45764.t1 MTR_8g107140 48.400 250 121 5 1 247 1 245 1.56e-70 219
MS.gene45764.t1 MTR_5g059200 45.882 255 125 6 1 252 1 245 3.01e-70 219
MS.gene45764.t1 MTR_5g023850 47.244 254 95 4 1 250 1 219 1.12e-69 216
MS.gene45764.t1 MTR_7g062610 46.639 238 123 3 11 247 8 242 3.96e-68 213
MS.gene45764.t1 MTR_3g068015 47.556 225 113 4 25 247 27 248 4.62e-67 210
MS.gene45764.t1 MTR_1g062690 45.968 248 102 7 4 249 3 220 6.51e-63 198
MS.gene45764.t1 MTR_6g009480 41.200 250 143 4 1 247 4 252 3.34e-61 196
MS.gene45764.t1 MTR_4g063630 42.324 241 127 8 13 246 16 251 1.95e-58 188
MS.gene45764.t1 MTR_2g067990 44.922 256 107 9 19 267 20 248 2.33e-58 201
MS.gene45764.t1 MTR_5g010640 40.000 255 126 9 1 247 1 236 2.89e-49 164
MS.gene45764.t1 MTR_2g067970 48.108 185 91 5 69 250 3 185 8.07e-48 171
MS.gene45764.t1 MTR_8g096900 41.004 239 115 9 11 247 34 248 7.03e-45 153
MS.gene45764.t1 MTR_5g010635 39.648 227 113 8 23 247 21 225 8.79e-44 150
MS.gene45764.t1 MTR_5g022350 50.345 145 67 3 6 150 77 216 2.03e-40 140
MS.gene45764.t1 MTR_8g089020 50.000 130 60 2 110 239 32 156 1.03e-36 129
MS.gene45764.t1 MTR_2g063150 34.272 213 131 5 26 236 34 239 3.84e-36 130
MS.gene45764.t1 MTR_7g076360 36.792 212 70 4 25 236 7 154 1.24e-32 119
MS.gene45764.t1 MTR_1g062390 31.621 253 152 8 1 247 1 238 6.12e-31 116
MS.gene45764.t1 MTR_8g096910 45.324 139 60 6 9 141 1 129 4.79e-29 109
MS.gene45764.t1 MTR_8g096920 41.304 138 70 5 9 144 1 129 1.01e-23 95.5
MS.gene45764.t1 MTR_3g081550 47.368 95 47 2 111 203 16 109 6.35e-19 80.9
MS.gene45764.t1 MTR_1g062640 36.364 99 60 2 70 168 51 146 9.50e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45764.t1 AT4G36010 69.099 233 66 3 22 248 19 251 4.50e-116 337
MS.gene45764.t1 AT4G36010 69.099 233 66 3 22 248 19 251 4.50e-116 337
MS.gene45764.t1 AT2G17860 63.563 247 85 3 9 250 6 252 3.27e-111 322
MS.gene45764.t1 AT1G75800 57.353 272 99 5 9 274 5 265 8.56e-108 317
MS.gene45764.t1 AT4G38670 60.547 256 93 5 9 257 6 260 2.94e-106 311
MS.gene45764.t1 AT4G38670 60.547 256 93 5 9 257 6 260 2.94e-106 311
MS.gene45764.t1 AT4G38670 66.222 225 71 4 27 247 8 231 1.60e-105 308
MS.gene45764.t1 AT1G20030 61.317 243 86 4 22 257 16 257 6.10e-104 306
MS.gene45764.t1 AT1G20030 61.667 240 84 4 25 257 2 240 9.16e-104 305
MS.gene45764.t1 AT4G38660 56.716 268 108 4 8 273 12 273 3.30e-99 295
MS.gene45764.t1 AT4G38660 58.203 256 99 4 20 273 2 251 1.20e-97 290
MS.gene45764.t1 AT4G24180 57.724 246 92 5 11 248 16 257 9.13e-96 283
MS.gene45764.t1 AT4G24180 57.895 247 93 5 9 248 10 252 1.22e-95 283
MS.gene45764.t1 AT4G24180 57.724 246 92 5 11 248 16 257 2.05e-95 285
MS.gene45764.t1 AT4G24180 57.895 247 93 5 9 248 10 252 2.44e-95 284
MS.gene45764.t1 AT5G24620 49.310 290 129 6 7 289 5 283 6.24e-89 271
MS.gene45764.t1 AT5G24620 49.310 290 129 6 7 289 60 338 2.47e-88 272
MS.gene45764.t1 AT5G24620 49.310 290 129 6 7 289 81 359 4.38e-88 272
MS.gene45764.t1 AT4G24180 55.652 230 90 5 11 232 16 241 3.66e-84 256
MS.gene45764.t1 AT4G24180 55.844 231 91 5 9 232 10 236 5.17e-84 256
MS.gene45764.t1 AT5G38280 50.207 241 116 2 11 247 10 250 2.24e-83 264
MS.gene45764.t1 AT5G38280 50.207 241 116 2 11 247 10 250 5.13e-83 263
MS.gene45764.t1 AT1G75030 53.629 248 104 8 9 250 4 246 1.18e-82 249
MS.gene45764.t1 AT1G19320 50.996 251 113 5 1 246 1 246 1.90e-80 244
MS.gene45764.t1 AT1G75040 53.750 240 105 4 8 247 6 239 1.42e-79 241
MS.gene45764.t1 AT1G75050 51.837 245 107 6 12 250 7 246 1.04e-78 239
MS.gene45764.t1 AT1G18250 51.542 227 103 4 23 247 19 240 8.75e-75 229
MS.gene45764.t1 AT1G18250 51.556 225 102 4 25 247 20 239 1.57e-74 229
MS.gene45764.t1 AT1G77700 48.954 239 108 6 12 246 43 271 3.18e-74 230
MS.gene45764.t1 AT1G77700 48.954 239 108 6 12 246 43 271 3.50e-74 231
MS.gene45764.t1 AT1G77700 51.351 222 98 5 25 246 88 299 1.70e-73 230
MS.gene45764.t1 AT1G73620 51.556 225 102 4 25 247 41 260 1.92e-73 227
MS.gene45764.t1 AT4G36000 64.780 159 47 3 102 259 1 151 2.54e-66 206
MS.gene45764.t1 AT4G36000 64.780 159 47 3 102 259 1 151 2.54e-66 206
MS.gene45764.t1 AT5G02140 44.223 251 131 4 8 257 6 248 7.36e-66 208
MS.gene45764.t1 AT5G02140 44.398 241 128 3 8 247 6 241 3.49e-65 205
MS.gene45764.t1 AT4G18253 44.262 244 125 4 9 249 5 240 3.81e-65 205
MS.gene45764.t1 AT2G28790 44.048 252 131 8 1 246 1 248 6.48e-62 197
MS.gene45764.t1 AT2G28790 44.048 252 131 8 1 246 26 273 1.36e-61 197
MS.gene45764.t1 AT1G70250 40.351 285 146 10 25 293 144 420 1.14e-60 206
MS.gene45764.t1 AT4G18250 44.898 245 124 6 7 249 39 274 1.14e-59 202
MS.gene45764.t1 AT5G40020 41.921 229 128 4 23 249 25 250 4.32e-59 190
MS.gene45764.t1 AT4G11650 40.458 262 129 10 11 267 5 244 1.48e-52 172
MS.gene45764.t1 AT5G24620 52.174 161 62 3 134 289 16 166 1.65e-45 156
MS.gene45764.t1 AT2G24810 61.039 77 30 0 170 246 116 192 3.24e-28 108

Find 92 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGGTATTGGAAGAGAATT+AGG 0.170473 8.3:-58777984 None:intergenic
CCAACAAGTTCGATCGATTC+TGG 0.203874 8.3:+58778720 MS.gene45764:CDS
GAACTTGGCTTGCATGTATT+TGG 0.239427 8.3:-58778552 None:intergenic
TCAAGTGGTCAGGCCGTCTA+TGG 0.272451 8.3:+58778154 MS.gene45764:CDS
GGAAAATTCTCTTGTGTTAC+CGG 0.309835 8.3:+58778207 MS.gene45764:CDS
CCCACAAGTTCGATCCAATC+TGG 0.346023 8.3:+58778684 MS.gene45764:CDS
AAAGCGCGTGTGAAGCGTTT+GGG 0.355433 8.3:+58778493 MS.gene45764:CDS
ATTCTCTTCCAATACCTAAC+TGG 0.364848 8.3:+58777988 MS.gene45764:CDS
GAGGGGTACGGCAAGGGCCT+TGG 0.365730 8.3:-58778133 None:intergenic
CCAGAATCGATCGAACTTGT+TGG 0.381756 8.3:-58778720 None:intergenic
TGTAGAGAACGGTGTTGTTC+CGG 0.383816 8.3:-58778082 None:intergenic
CCGGCGCCGGATAAAATTCC+AGG 0.384166 8.3:-58778063 None:intergenic
CATATGAACCAGTTAGGTAT+TGG 0.386121 8.3:-58777996 None:intergenic
CCATGTGGTTCGATCATCAT+TGG 0.396722 8.3:-58778363 None:intergenic
AACTCAACCAACCATCGCTT+TGG 0.401111 8.3:+58777957 MS.gene45764:CDS
TTAGCCGAGTTTACACTTAA+CGG 0.420790 8.3:+58778288 MS.gene45764:CDS
CTCATCACTTTCAACTCGGT+TGG 0.422630 8.3:-58778447 None:intergenic
GAGATTTCTCGTTCCCAGAT+TGG 0.423607 8.3:-58778698 None:intergenic
ACGAGTGCAACTACACAGTT+TGG 0.425166 8.3:+58778040 MS.gene45764:CDS
ATCGAACCACATGGAGCAAC+CGG 0.428774 8.3:+58778372 MS.gene45764:CDS
TGTCATGCAGTTTCCGCCTC+CGG 0.443562 8.3:-58778391 None:intergenic
TTGTTGCATATGAACCAGTT+AGG 0.449950 8.3:-58778002 None:intergenic
AAAAGCGCGTGTGAAGCGTT+TGG 0.451521 8.3:+58778492 MS.gene45764:CDS
GGGCCTTGGATTCGCCGGAT+TGG 0.458692 8.3:-58778119 None:intergenic
CAAGTGGTCAGGCCGTCTAT+GGG 0.458980 8.3:+58778155 MS.gene45764:CDS
ACAACACCGTTCTCTACAAC+CGG 0.459009 8.3:+58778087 MS.gene45764:CDS
GCCGTACCCCTCAAGTGGTC+AGG 0.460490 8.3:+58778144 MS.gene45764:CDS
ATCTCCATTGCCTGAGGAGA+TGG 0.461012 8.3:+58778752 MS.gene45764:CDS
CAACAAGTTCGATCGATTCT+GGG 0.463678 8.3:+58778721 MS.gene45764:CDS
GTTTCCTCCAGCACACTCTA+TGG 0.465520 8.3:-58778247 None:intergenic
CCACATGGAGCAACCGGAGG+CGG 0.465833 8.3:+58778378 MS.gene45764:CDS
GCCGGATTGGAGAGCAAAGC+CGG 0.466764 8.3:-58778106 None:intergenic
TAGGTATTGGAAGAGAATTA+GGG 0.467250 8.3:-58777983 None:intergenic
GGCGGAAACTGCATGACAAC+AGG 0.472230 8.3:+58778396 MS.gene45764:CDS
TATAGCTACGCTTATGATGA+TGG 0.472777 8.3:+58778612 MS.gene45764:CDS
TCCTCCACCATAGAGTGTGC+TGG 0.480343 8.3:+58778240 MS.gene45764:CDS
CCAGATTGGATCGAACTTGT+GGG 0.490228 8.3:-58778684 None:intergenic
TTTACACTTAACGGTGCAAA+CGG 0.497247 8.3:+58778297 MS.gene45764:CDS
CCCAGATTGGATCGAACTTG+TGG 0.498644 8.3:-58778685 None:intergenic
CATGTGGTTCGATCATCATT+GGG 0.503855 8.3:-58778362 None:intergenic
GGCAAGGGCCTTGGATTCGC+CGG 0.509446 8.3:-58778124 None:intergenic
GAACGGTGTTGTTCCGGCGC+CGG 0.511342 8.3:-58778076 None:intergenic
AAAATAAACTAATTGAAAAG+TGG 0.514682 8.3:-58778930 None:intergenic
GTAAACTCGGCTAATGTAGC+TGG 0.516844 8.3:-58778279 None:intergenic
ACCGGCTTTGCTCTCCAATC+CGG 0.521392 8.3:+58778105 MS.gene45764:CDS
TGCAACTACACAGTTTGGCC+TGG 0.523345 8.3:+58778045 MS.gene45764:CDS
ATATCCATCTCCTCAGGCAA+TGG 0.531593 8.3:-58778756 None:intergenic
TCTCCAATCCGGCGAATCCA+AGG 0.538634 8.3:+58778116 MS.gene45764:CDS
GGATATATCTGCGGGACATG+TGG 0.539107 8.3:+58778773 MS.gene45764:CDS
CCACTTGAGGGGTACGGCAA+GGG 0.539972 8.3:-58778139 None:intergenic
CCACAAGTTCGATCCAATCT+GGG 0.541949 8.3:+58778685 MS.gene45764:CDS
TAGACGGCCTGACCACTTGA+GGG 0.545483 8.3:-58778151 None:intergenic
ACCACTTGAGGGGTACGGCA+AGG 0.548954 8.3:-58778140 None:intergenic
AGGGTAACCAAAGCGATGGT+TGG 0.551440 8.3:-58777964 None:intergenic
AAGCGCGTGTGAAGCGTTTG+GGG 0.554522 8.3:+58778494 MS.gene45764:CDS
GCAGATATATCCATCTCCTC+AGG 0.555938 8.3:-58778762 None:intergenic
GCCTGACCACTTGAGGGGTA+CGG 0.559570 8.3:-58778145 None:intergenic
AGAGGGTACGTCCCCATAGA+CGG 0.562547 8.3:-58778167 None:intergenic
CCGCCTCCGGTTGCTCCATG+TGG 0.564023 8.3:-58778378 None:intergenic
TCCAGCACACTCTATGGTGG+AGG 0.567323 8.3:-58778241 None:intergenic
ATAGACGGCCTGACCACTTG+AGG 0.567345 8.3:-58778152 None:intergenic
CTCTGCTCTCAAGATTCCAC+CGG 0.569324 8.3:+58778186 MS.gene45764:CDS
GAGGAGATGGATATATCTGC+GGG 0.571439 8.3:+58778765 MS.gene45764:CDS
CTGCATGACAACAGGTTGCA+TGG 0.576620 8.3:+58778404 MS.gene45764:CDS
GGTGGAGGAAGCACAGTCAC+CGG 0.577174 8.3:-58778226 None:intergenic
GATCCACAATATTGTTGTAG+TGG 0.578754 8.3:+58778516 MS.gene45764:CDS
TGCACCGTTAAGTGTAAACT+CGG 0.588205 8.3:-58778292 None:intergenic
ATTACTCATCACTTTCAACT+CGG 0.591426 8.3:-58778451 None:intergenic
CCCTTGCCGTACCCCTCAAG+TGG 0.601779 8.3:+58778139 MS.gene45764:CDS
CGGTGGAATCTTGAGAGCAG+AGG 0.605968 8.3:-58778185 None:intergenic
GCAAAGCCGGTTGTAGAGAA+CGG 0.610915 8.3:-58778093 None:intergenic
TCCTCCAGCACACTCTATGG+TGG 0.611738 8.3:-58778244 None:intergenic
GCGCCACTACAACAATATTG+TGG 0.613506 8.3:-58778519 None:intergenic
GAGTTGAAAGTGATGAGTAA+TGG 0.615914 8.3:+58778453 MS.gene45764:CDS
GGTGGAATCTTGAGAGCAGA+GGG 0.624367 8.3:-58778184 None:intergenic
TTTCTTCGACGTTAGTCACG+TGG 0.627829 8.3:+58778326 MS.gene45764:CDS
AATTAGGGTAACCAAAGCGA+TGG 0.636779 8.3:-58777968 None:intergenic
TGAGGAGATGGATATATCTG+CGG 0.639368 8.3:+58778764 MS.gene45764:CDS
TCCACCATAGAGTGTGCTGG+AGG 0.646324 8.3:+58778243 MS.gene45764:CDS
AGACGGCCTGACCACTTGAG+GGG 0.648811 8.3:-58778150 None:intergenic
TTCGACGTTAGTCACGTGGA+CGG 0.649300 8.3:+58778330 MS.gene45764:CDS
AAGTGGTCAGGCCGTCTATG+GGG 0.652593 8.3:+58778156 MS.gene45764:CDS
GTGGTTCGATCATCATTGGG+AGG 0.653259 8.3:-58778359 None:intergenic
CCAATGATGATCGAACCACA+TGG 0.667726 8.3:+58778363 MS.gene45764:CDS
TGAGAAATCTCCATTGCCTG+AGG 0.668209 8.3:+58778746 MS.gene45764:CDS
AACTCGGCTAATGTAGCTGG+CGG 0.669689 8.3:-58778276 None:intergenic
GAACCACATGGAGCAACCGG+AGG 0.670618 8.3:+58778375 MS.gene45764:CDS
TTGAAAGTGATGAGTAATGG+CGG 0.675133 8.3:+58778456 MS.gene45764:CDS
TTGCATGGTGGATTTGAATG+CGG 0.687441 8.3:+58778419 MS.gene45764:CDS
AAAGTGATGAGTAATGGCGG+AGG 0.693219 8.3:+58778459 MS.gene45764:CDS
CATGACAACAGGTTGCATGG+TGG 0.757398 8.3:+58778407 MS.gene45764:CDS
TAAGCGTAGCTATAAGCACG+AGG 0.764294 8.3:-58778603 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTTCTTTTGTTTTTT+TGG + chr8.3:58778827-58778846 MS.gene45764:intron 10.0%
!!! TTTTTGTTTTTTTTTTTTGA+GGG + chr8.3:58778851-58778870 MS.gene45764:intron 10.0%
!!! TTTTTTGTTTTTTTTTTTTG+AGG + chr8.3:58778850-58778869 MS.gene45764:intron 10.0%
!! AACAAAAATCATAACATTGT+TGG - chr8.3:58778808-58778827 None:intergenic 20.0%
!!! CTTTTGTTTTGATGTTGTTT+TGG + chr8.3:58778881-58778900 MS.gene45764:intron 25.0%
AAAAATTGCGAGTATGAACT+TGG - chr8.3:58778570-58778589 None:intergenic 30.0%
ATTACTCATCACTTTCAACT+CGG - chr8.3:58778454-58778473 None:intergenic 30.0%
!! TAGGTATTGGAAGAGAATTA+GGG - chr8.3:58777986-58778005 None:intergenic 30.0%
!! TTAGGTATTGGAAGAGAATT+AGG - chr8.3:58777987-58778006 None:intergenic 30.0%
!!! TTGTTTTGATGTTGTTTTGG+AGG + chr8.3:58778884-58778903 MS.gene45764:intron 30.0%
ATTCTCTTCCAATACCTAAC+TGG + chr8.3:58777988-58778007 MS.gene45764:CDS 35.0%
CATATGAACCAGTTAGGTAT+TGG - chr8.3:58777999-58778018 None:intergenic 35.0%
GAGTTGAAAGTGATGAGTAA+TGG + chr8.3:58778453-58778472 MS.gene45764:CDS 35.0%
GATCCACAATATTGTTGTAG+TGG + chr8.3:58778516-58778535 MS.gene45764:CDS 35.0%
GGAAAATTCTCTTGTGTTAC+CGG + chr8.3:58778207-58778226 MS.gene45764:CDS 35.0%
TATAGCTACGCTTATGATGA+TGG + chr8.3:58778612-58778631 MS.gene45764:CDS 35.0%
TTAGCCGAGTTTACACTTAA+CGG + chr8.3:58778288-58778307 MS.gene45764:CDS 35.0%
TTGAAAGTGATGAGTAATGG+CGG + chr8.3:58778456-58778475 MS.gene45764:CDS 35.0%
TTGTTGCATATGAACCAGTT+AGG - chr8.3:58778005-58778024 None:intergenic 35.0%
TTTACACTTAACGGTGCAAA+CGG + chr8.3:58778297-58778316 MS.gene45764:CDS 35.0%
! GGTAACACAAGAGAATTTTC+CGG - chr8.3:58778208-58778227 None:intergenic 35.0%
!!! TGTTGTTTTGGAGGTGAATT+TGG + chr8.3:58778893-58778912 MS.gene45764:intron 35.0%
AATTAGGGTAACCAAAGCGA+TGG - chr8.3:58777971-58777990 None:intergenic 40.0%
ACTTGTGGGACAAAAAGTGA+TGG - chr8.3:58778673-58778692 None:intergenic 40.0%
CATGTGGTTCGATCATCATT+GGG - chr8.3:58778365-58778384 None:intergenic 40.0%
TGAGGAGATGGATATATCTG+CGG + chr8.3:58778764-58778783 MS.gene45764:CDS 40.0%
TGCACCGTTAAGTGTAAACT+CGG - chr8.3:58778295-58778314 None:intergenic 40.0%
! AACACAAGAGAATTTTCCGG+TGG - chr8.3:58778205-58778224 None:intergenic 40.0%
! CAACAAGTTCGATCGATTCT+GGG + chr8.3:58778721-58778740 MS.gene45764:CDS 40.0%
! GTTTGGCCTGGAATTTTATC+CGG + chr8.3:58778057-58778076 MS.gene45764:CDS 40.0%
!! GAACTTGGCTTGCATGTATT+TGG - chr8.3:58778555-58778574 None:intergenic 40.0%
!! TTGCATGGTGGATTTGAATG+CGG + chr8.3:58778419-58778438 MS.gene45764:CDS 40.0%
!!! GTTTTGGAGGTGAATTTGGT+AGG + chr8.3:58778897-58778916 MS.gene45764:intron 40.0%
AAAGTGATGAGTAATGGCGG+AGG + chr8.3:58778459-58778478 MS.gene45764:CDS 45.0%
AACTCAACCAACCATCGCTT+TGG + chr8.3:58777957-58777976 MS.gene45764:CDS 45.0%
ACAACACCGTTCTCTACAAC+CGG + chr8.3:58778087-58778106 MS.gene45764:CDS 45.0%
ACGAGTGCAACTACACAGTT+TGG + chr8.3:58778040-58778059 MS.gene45764:CDS 45.0%
ATATCCATCTCCTCAGGCAA+TGG - chr8.3:58778759-58778778 None:intergenic 45.0%
CCAATGATGATCGAACCACA+TGG + chr8.3:58778363-58778382 MS.gene45764:CDS 45.0%
CCACAAGTTCGATCCAATCT+GGG + chr8.3:58778685-58778704 MS.gene45764:CDS 45.0%
CCAGAATCGATCGAACTTGT+TGG - chr8.3:58778723-58778742 None:intergenic 45.0%
CCAGATTGGATCGAACTTGT+GGG - chr8.3:58778687-58778706 None:intergenic 45.0%
CCATGTGGTTCGATCATCAT+TGG - chr8.3:58778366-58778385 None:intergenic 45.0%
CTCATCACTTTCAACTCGGT+TGG - chr8.3:58778450-58778469 None:intergenic 45.0%
GAGATTTCTCGTTCCCAGAT+TGG - chr8.3:58778701-58778720 None:intergenic 45.0%
GAGGAGATGGATATATCTGC+GGG + chr8.3:58778765-58778784 MS.gene45764:CDS 45.0%
GCAGATATATCCATCTCCTC+AGG - chr8.3:58778765-58778784 None:intergenic 45.0%
GCGCCACTACAACAATATTG+TGG - chr8.3:58778522-58778541 None:intergenic 45.0%
GTAAACTCGGCTAATGTAGC+TGG - chr8.3:58778282-58778301 None:intergenic 45.0%
TAAGCGTAGCTATAAGCACG+AGG - chr8.3:58778606-58778625 None:intergenic 45.0%
TGAGAAATCTCCATTGCCTG+AGG + chr8.3:58778746-58778765 MS.gene45764:CDS 45.0%
TTTCTTCGACGTTAGTCACG+TGG + chr8.3:58778326-58778345 MS.gene45764:CDS 45.0%
! CCAACAAGTTCGATCGATTC+TGG + chr8.3:58778720-58778739 MS.gene45764:CDS 45.0%
!! TGTAGAGAACGGTGTTGTTC+CGG - chr8.3:58778085-58778104 None:intergenic 45.0%
AACTCGGCTAATGTAGCTGG+CGG - chr8.3:58778279-58778298 None:intergenic 50.0%
AGGGTAACCAAAGCGATGGT+TGG - chr8.3:58777967-58777986 None:intergenic 50.0%
ATCGAACCACATGGAGCAAC+CGG + chr8.3:58778372-58778391 MS.gene45764:CDS 50.0%
ATCTCCATTGCCTGAGGAGA+TGG + chr8.3:58778752-58778771 MS.gene45764:CDS 50.0%
CATGACAACAGGTTGCATGG+TGG + chr8.3:58778407-58778426 MS.gene45764:CDS 50.0%
CCCACAAGTTCGATCCAATC+TGG + chr8.3:58778684-58778703 MS.gene45764:CDS 50.0%
CCCAGATTGGATCGAACTTG+TGG - chr8.3:58778688-58778707 None:intergenic 50.0%
CTCTGCTCTCAAGATTCCAC+CGG + chr8.3:58778186-58778205 MS.gene45764:CDS 50.0%
CTGCATGACAACAGGTTGCA+TGG + chr8.3:58778404-58778423 MS.gene45764:CDS 50.0%
GCAAAGCCGGTTGTAGAGAA+CGG - chr8.3:58778096-58778115 None:intergenic 50.0%
GGATATATCTGCGGGACATG+TGG + chr8.3:58778773-58778792 MS.gene45764:CDS 50.0%
GGTGGAATCTTGAGAGCAGA+GGG - chr8.3:58778187-58778206 None:intergenic 50.0%
GTGGTTCGATCATCATTGGG+AGG - chr8.3:58778362-58778381 None:intergenic 50.0%
GTTTCCTCCAGCACACTCTA+TGG - chr8.3:58778250-58778269 None:intergenic 50.0%
TGCAACTACACAGTTTGGCC+TGG + chr8.3:58778045-58778064 MS.gene45764:CDS 50.0%
TTCGACGTTAGTCACGTGGA+CGG + chr8.3:58778330-58778349 MS.gene45764:CDS 50.0%
! AAAAGCGCGTGTGAAGCGTT+TGG + chr8.3:58778492-58778511 MS.gene45764:CDS 50.0%
! AAAGCGCGTGTGAAGCGTTT+GGG + chr8.3:58778493-58778512 MS.gene45764:CDS 50.0%
AAGTGGTCAGGCCGTCTATG+GGG + chr8.3:58778156-58778175 MS.gene45764:CDS 55.0%
ATAGACGGCCTGACCACTTG+AGG - chr8.3:58778155-58778174 None:intergenic 55.0%
CAAGTGGTCAGGCCGTCTAT+GGG + chr8.3:58778155-58778174 MS.gene45764:CDS 55.0%
CGGTGGAATCTTGAGAGCAG+AGG - chr8.3:58778188-58778207 None:intergenic 55.0%
GGCGGAAACTGCATGACAAC+AGG + chr8.3:58778396-58778415 MS.gene45764:CDS 55.0%
TAGACGGCCTGACCACTTGA+GGG - chr8.3:58778154-58778173 None:intergenic 55.0%
TCAAGTGGTCAGGCCGTCTA+TGG + chr8.3:58778154-58778173 MS.gene45764:CDS 55.0%
TCCACCATAGAGTGTGCTGG+AGG + chr8.3:58778243-58778262 MS.gene45764:CDS 55.0%
TCCAGCACACTCTATGGTGG+AGG - chr8.3:58778244-58778263 None:intergenic 55.0%
TCCTCCACCATAGAGTGTGC+TGG + chr8.3:58778240-58778259 MS.gene45764:CDS 55.0%
TCCTCCAGCACACTCTATGG+TGG - chr8.3:58778247-58778266 None:intergenic 55.0%
TCTCCAATCCGGCGAATCCA+AGG + chr8.3:58778116-58778135 MS.gene45764:CDS 55.0%
TGTCATGCAGTTTCCGCCTC+CGG - chr8.3:58778394-58778413 None:intergenic 55.0%
! AAGCGCGTGTGAAGCGTTTG+GGG + chr8.3:58778494-58778513 MS.gene45764:CDS 55.0%
! ACCGGCTTTGCTCTCCAATC+CGG + chr8.3:58778105-58778124 MS.gene45764:CDS 55.0%
! AGAGGGTACGTCCCCATAGA+CGG - chr8.3:58778170-58778189 None:intergenic 55.0%
! CCTGGAATTTTATCCGGCGC+CGG + chr8.3:58778063-58778082 MS.gene45764:CDS 55.0%
AGACGGCCTGACCACTTGAG+GGG - chr8.3:58778153-58778172 None:intergenic 60.0%
GAACCACATGGAGCAACCGG+AGG + chr8.3:58778375-58778394 MS.gene45764:CDS 60.0%
GCCTGACCACTTGAGGGGTA+CGG - chr8.3:58778148-58778167 None:intergenic 60.0%
! ACCACTTGAGGGGTACGGCA+AGG - chr8.3:58778143-58778162 None:intergenic 60.0%
! CCACTTGAGGGGTACGGCAA+GGG - chr8.3:58778142-58778161 None:intergenic 60.0%
! CCGGCGCCGGATAAAATTCC+AGG - chr8.3:58778066-58778085 None:intergenic 60.0%
! GCCGGATTGGAGAGCAAAGC+CGG - chr8.3:58778109-58778128 None:intergenic 60.0%
! GGTGGAGGAAGCACAGTCAC+CGG - chr8.3:58778229-58778248 None:intergenic 60.0%
CCACATGGAGCAACCGGAGG+CGG + chr8.3:58778378-58778397 MS.gene45764:CDS 65.0%
CCCTTGCCGTACCCCTCAAG+TGG + chr8.3:58778139-58778158 MS.gene45764:CDS 65.0%
GCCGTACCCCTCAAGTGGTC+AGG + chr8.3:58778144-58778163 MS.gene45764:CDS 65.0%
GGCAAGGGCCTTGGATTCGC+CGG - chr8.3:58778127-58778146 None:intergenic 65.0%
GGGCCTTGGATTCGCCGGAT+TGG - chr8.3:58778122-58778141 None:intergenic 65.0%
!! GAACGGTGTTGTTCCGGCGC+CGG - chr8.3:58778079-58778098 None:intergenic 65.0%
CCGCCTCCGGTTGCTCCATG+TGG - chr8.3:58778381-58778400 None:intergenic 70.0%
! GAGGGGTACGGCAAGGGCCT+TGG - chr8.3:58778136-58778155 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 58777946 58778944 58777946 ID=MS.gene45764
chr8.3 mRNA 58777946 58778944 58777946 ID=MS.gene45764.t1;Parent=MS.gene45764
chr8.3 exon 58777946 58778812 58777946 ID=MS.gene45764.t1.exon1;Parent=MS.gene45764.t1
chr8.3 CDS 58777946 58778812 58777946 ID=cds.MS.gene45764.t1;Parent=MS.gene45764.t1
chr8.3 exon 58778906 58778944 58778906 ID=MS.gene45764.t1.exon2;Parent=MS.gene45764.t1
chr8.3 CDS 58778906 58778944 58778906 ID=cds.MS.gene45764.t1;Parent=MS.gene45764.t1
Gene Sequence

>MS.gene45764

ATGGCTGCTTCAACTCAACCAACCATCGCTTTGGTTACCCTAATTCTCTTCCAATACCTAACTGGTTCATATGCAACAACATTCACAATTGTTAACGAGTGCAACTACACAGTTTGGCCTGGAATTTTATCCGGCGCCGGAACAACACCGTTCTCTACAACCGGCTTTGCTCTCCAATCCGGCGAATCCAAGGCCCTTGCCGTACCCCTCAAGTGGTCAGGCCGTCTATGGGGACGTACCCTCTGCTCTCAAGATTCCACCGGAAAATTCTCTTGTGTTACCGGTGACTGTGCTTCCTCCACCATAGAGTGTGCTGGAGGAAACGCTCAACCGCCAGCTACATTAGCCGAGTTTACACTTAACGGTGCAAACGGACTAGATTTCTTCGACGTTAGTCACGTGGACGGTTATAACCTCCCAATGATGATCGAACCACATGGAGCAACCGGAGGCGGAAACTGCATGACAACAGGTTGCATGGTGGATTTGAATGCGGCGTGTCCAACCGAGTTGAAAGTGATGAGTAATGGCGGAGGTGTAGCGTGTAAAAGCGCGTGTGAAGCGTTTGGGGATCCACAATATTGTTGTAGTGGCGCTTACGCTTCTCCAAATACATGCAAGCCAAGTTCATACTCGCAATTTTTCAAGAATGCTTGTCCTCGTGCTTATAGCTACGCTTATGATGATGGAACAAGCACTTTTACTTGTGATTCTGCTGATTATACCATCACTTTTTGTCCCACAAGTTCGATCCAATCTGGGAACGAGAAATCTCCAACAAGTTCGATCGATTCTGGGAATGAGAAATCTCCATTGCCTGAGGAGATGGATATATCTGCGGGACATGTGGACTTCACAGCCAACAATGTTATGATTTTTGTTATTATTTCTTTTGTTTTTTTGGTTTTTTGTTTTTTTTTTTTGAGGGATTATTTCTTTTGTTTTGATGTTGTTTTGGAGGTGAATTTGGTAGGCTATGACTTGCCACTTTTCAATTAG
Protein sequence

>MS.gene45764.t1

MAASTQPTIALVTLILFQYLTGSYATTFTIVNECNYTVWPGILSGAGTTPFSTTGFALQSGESKALAVPLKWSGRLWGRTLCSQDSTGKFSCVTGDCASSTIECAGGNAQPPATLAEFTLNGANGLDFFDVSHVDGYNLPMMIEPHGATGGGNCMTTGCMVDLNAACPTELKVMSNGGGVACKSACEAFGDPQYCCSGAYASPNTCKPSSYSQFFKNACPRAYSYAYDDGTSTFTCDSADYTITFCPTSSIQSGNEKSPTSSIDSGNEKSPLPEEMDISAGHVDFTANNVNLVGYDLPLFN