AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68072

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68072.t1 MTR_5g022310 94.426 305 12 2 1 305 1 300 0.0 581
MS.gene68072.t1 MTR_5g022310 95.703 256 9 1 1 256 1 254 9.79e-180 496
MS.gene68072.t1 MTR_8g037890 71.517 323 62 6 1 300 1 316 6.74e-157 441
MS.gene68072.t1 MTR_8g056820 80.077 261 46 1 1 261 1 255 1.54e-150 422
MS.gene68072.t1 MTR_8g075550 68.106 301 89 4 1 296 1 299 6.65e-138 392
MS.gene68072.t1 MTR_1g025420 67.717 254 75 4 14 266 11 258 1.15e-118 344
MS.gene68072.t1 MTR_1g025420 67.742 248 74 3 14 260 11 253 2.53e-117 338
MS.gene68072.t1 MTR_3g114030 70.417 240 65 3 20 258 1 235 5.61e-117 337
MS.gene68072.t1 MTR_4g073730 60.377 265 96 3 11 270 5 265 1.86e-106 312
MS.gene68072.t1 MTR_4g073730 55.705 298 115 5 11 295 5 298 2.93e-106 313
MS.gene68072.t1 MTR_4g073720 57.613 243 87 4 14 252 9 239 1.07e-90 273
MS.gene68072.t1 MTR_5g022350 53.137 271 109 6 1 268 4 259 5.82e-89 268
MS.gene68072.t1 MTR_8g075510 54.118 255 99 7 3 256 10 247 2.99e-84 254
MS.gene68072.t1 MTR_8g075510 54.183 251 97 7 3 252 10 243 6.37e-83 253
MS.gene68072.t1 MTR_7g102380 49.801 251 114 5 4 253 2 241 1.71e-81 246
MS.gene68072.t1 MTR_2g068030 50.435 230 104 4 25 252 22 243 1.18e-78 240
MS.gene68072.t1 MTR_2g069660 52.653 245 107 5 11 253 6 243 1.62e-78 239
MS.gene68072.t1 MTR_2g068655 50.644 233 107 3 22 253 27 252 3.29e-78 241
MS.gene68072.t1 MTR_8g036215 50.407 246 113 5 7 252 1 237 4.80e-78 238
MS.gene68072.t1 MTR_1g062590 49.200 250 116 5 7 253 1 242 9.52e-78 237
MS.gene68072.t1 MTR_6g079580 48.996 249 112 5 11 253 1 240 2.05e-75 231
MS.gene68072.t1 MTR_2g067980 47.104 259 123 5 10 262 6 256 2.10e-75 249
MS.gene68072.t1 MTR_1g062630 50.988 253 109 6 7 254 1 243 4.87e-75 231
MS.gene68072.t1 MTR_1g062660 50.410 244 108 6 12 254 12 243 9.67e-75 229
MS.gene68072.t1 MTR_8g107140 50.417 240 110 4 14 253 15 245 1.06e-74 229
MS.gene68072.t1 MTR_1g062340 49.794 243 109 6 13 254 13 243 3.13e-74 228
MS.gene68072.t1 MTR_3g111620 53.247 231 98 5 28 257 29 250 9.12e-74 227
MS.gene68072.t1 MTR_1g021945 51.064 235 103 5 25 257 25 249 3.18e-73 226
MS.gene68072.t1 MTR_1g062350 48.617 253 118 6 3 254 2 243 6.83e-73 224
MS.gene68072.t1 MTR_1g062370 47.826 253 120 6 3 254 2 243 2.05e-72 223
MS.gene68072.t1 MTR_7g062610 44.664 253 130 4 5 256 2 245 3.07e-69 215
MS.gene68072.t1 MTR_5g023850 45.455 264 99 5 1 260 1 223 3.68e-69 214
MS.gene68072.t1 MTR_5g059200 46.531 245 111 7 12 252 11 239 1.14e-67 213
MS.gene68072.t1 MTR_3g068015 45.652 230 114 5 26 253 28 248 1.23e-63 201
MS.gene68072.t1 MTR_6g009480 41.176 255 142 5 1 253 4 252 4.82e-62 198
MS.gene68072.t1 MTR_1g062690 43.083 253 108 7 3 254 2 219 3.49e-57 184
MS.gene68072.t1 MTR_4g063630 41.564 243 130 8 14 252 17 251 5.54e-56 182
MS.gene68072.t1 MTR_2g067990 42.562 242 105 7 22 262 23 231 1.50e-53 188
MS.gene68072.t1 MTR_5g010640 38.314 261 128 9 1 253 1 236 6.39e-47 158
MS.gene68072.t1 MTR_5g010635 39.914 233 110 8 23 253 21 225 1.49e-46 157
MS.gene68072.t1 MTR_2g067970 45.026 191 94 6 69 256 3 185 4.44e-46 166
MS.gene68072.t1 MTR_8g096900 38.645 251 119 9 9 253 27 248 6.09e-45 153
MS.gene68072.t1 MTR_5g022350 48.125 160 75 4 1 158 69 222 2.46e-39 138
MS.gene68072.t1 MTR_2g063150 34.545 220 131 5 27 245 35 242 3.09e-39 139
MS.gene68072.t1 MTR_8g089020 48.889 135 58 3 111 245 33 156 3.04e-36 128
MS.gene68072.t1 MTR_7g076360 35.616 219 71 4 25 243 7 155 4.71e-32 117
MS.gene68072.t1 MTR_1g062390 32.432 259 148 10 1 253 1 238 4.90e-31 117
MS.gene68072.t1 MTR_8g096910 43.165 139 61 6 10 141 2 129 1.10e-26 103
MS.gene68072.t1 MTR_3g081550 48.544 103 48 2 107 209 12 109 5.61e-22 89.4
MS.gene68072.t1 MTR_8g096920 41.176 136 69 5 10 143 2 128 9.54e-22 90.5
MS.gene68072.t1 MTR_1g062640 42.222 90 49 2 79 168 60 146 4.69e-16 74.3
MS.gene68072.t1 MTR_2g063160 36.170 94 56 3 30 121 18 109 1.85e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68072.t1 AT4G36010 59.028 288 107 4 22 304 19 300 4.78e-116 337
MS.gene68072.t1 AT4G36010 59.028 288 107 4 22 304 19 300 4.78e-116 337
MS.gene68072.t1 AT1G75800 62.977 262 91 5 11 270 7 264 1.19e-112 329
MS.gene68072.t1 AT2G17860 63.306 248 84 4 11 256 10 252 5.82e-110 319
MS.gene68072.t1 AT1G20030 66.667 240 72 4 22 258 16 250 2.12e-108 318
MS.gene68072.t1 AT1G20030 67.089 237 70 4 25 258 2 233 5.84e-108 316
MS.gene68072.t1 AT4G38670 64.050 242 80 4 27 266 24 260 8.51e-105 307
MS.gene68072.t1 AT4G38670 64.050 242 80 4 27 266 24 260 8.51e-105 307
MS.gene68072.t1 AT4G38670 64.050 242 80 4 27 266 8 244 1.90e-104 306
MS.gene68072.t1 AT4G38660 53.472 288 108 5 1 274 2 277 1.20e-97 291
MS.gene68072.t1 AT4G38660 55.805 267 95 4 19 274 1 255 4.20e-97 289
MS.gene68072.t1 AT4G24180 54.945 273 102 7 1 263 6 267 2.50e-92 277
MS.gene68072.t1 AT4G24180 55.513 263 97 6 1 254 6 257 4.51e-92 274
MS.gene68072.t1 AT4G24180 54.982 271 100 7 1 263 6 262 4.79e-91 273
MS.gene68072.t1 AT4G24180 55.556 261 95 6 1 254 6 252 8.91e-91 271
MS.gene68072.t1 AT5G24620 55.918 245 104 4 11 253 9 251 4.11e-87 267
MS.gene68072.t1 AT5G24620 55.918 245 104 4 11 253 64 306 8.60e-87 268
MS.gene68072.t1 AT5G24620 55.918 245 104 4 11 253 85 327 1.32e-86 268
MS.gene68072.t1 AT5G38280 51.181 254 119 3 7 258 5 255 2.34e-85 270
MS.gene68072.t1 AT5G38280 51.181 254 119 3 7 258 5 255 4.93e-85 269
MS.gene68072.t1 AT4G24180 53.441 247 95 6 1 238 6 241 1.36e-80 247
MS.gene68072.t1 AT1G75040 53.086 243 102 5 11 253 9 239 1.07e-79 242
MS.gene68072.t1 AT4G24180 53.469 245 93 6 1 238 6 236 1.80e-79 244
MS.gene68072.t1 AT1G19320 50.813 246 107 5 11 252 11 246 2.14e-79 241
MS.gene68072.t1 AT1G75030 49.798 247 114 6 11 256 9 246 1.77e-77 236
MS.gene68072.t1 AT1G75050 49.606 254 118 6 1 254 1 244 3.37e-77 236
MS.gene68072.t1 AT1G73620 52.402 229 100 4 25 253 41 260 3.80e-76 234
MS.gene68072.t1 AT1G18250 51.515 231 103 4 23 253 19 240 4.07e-75 230
MS.gene68072.t1 AT1G18250 51.528 229 102 4 25 253 20 239 6.15e-75 230
MS.gene68072.t1 AT1G77700 47.755 245 108 7 12 252 43 271 2.50e-70 221
MS.gene68072.t1 AT1G77700 47.347 245 109 7 12 252 43 271 2.99e-70 220
MS.gene68072.t1 AT1G77700 49.561 228 99 6 25 252 88 299 8.53e-70 221
MS.gene68072.t1 AT4G36000 65.089 169 41 4 102 270 1 151 3.59e-67 209
MS.gene68072.t1 AT4G36000 65.089 169 41 4 102 270 1 151 3.59e-67 209
MS.gene68072.t1 AT5G02140 40.268 298 145 7 9 297 2 275 2.78e-64 204
MS.gene68072.t1 AT5G02140 43.548 248 129 4 9 253 2 241 4.75e-64 202
MS.gene68072.t1 AT2G28790 42.913 254 132 7 3 252 4 248 5.74e-61 194
MS.gene68072.t1 AT2G28790 42.913 254 132 7 3 252 29 273 6.92e-61 195
MS.gene68072.t1 AT4G18253 42.629 251 127 5 11 258 7 243 1.62e-59 191
MS.gene68072.t1 AT5G40020 41.702 235 128 5 21 254 23 249 3.95e-59 190
MS.gene68072.t1 AT1G70250 44.118 238 118 7 25 257 144 371 1.15e-58 201
MS.gene68072.t1 AT4G18250 39.394 297 160 9 11 302 40 321 3.73e-55 190
MS.gene68072.t1 AT4G11650 39.442 251 120 9 7 253 1 223 3.42e-49 164
MS.gene68072.t1 AT5G24620 61.983 121 45 1 133 253 15 134 2.79e-44 153
MS.gene68072.t1 AT2G24810 56.627 83 30 1 170 252 116 192 4.80e-26 102

Find 97 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTGGTCTGGCCGTCTA+TGG 0.191862 5.3:+12992100 MS.gene68072:CDS
TTAGGAGTTGGTAGAGAATA+AGG 0.252939 5.3:-12991718 None:intergenic
TGGCGGGAGTGTTACCTATT+AGG 0.288934 5.3:-12991736 None:intergenic
TATTGCTGCAGTGGCGCTTA+TGG 0.293843 5.3:+12992489 MS.gene68072:CDS
GAATGCAGTTCAATCAAATC+TGG 0.324954 5.3:+12993260 MS.gene68072:intron
CTCTCGCCGTACCACCTTCC+TGG 0.337002 5.3:+12992085 MS.gene68072:CDS
ATTCTCTACCAACTCCTAAT+AGG 0.344279 5.3:+12991722 MS.gene68072:CDS
AAAGCGCGTGTGAAGCGTTT+GGG 0.355433 5.3:+12992457 MS.gene68072:CDS
CCGTGTGGTTCGATCAATAT+CGG 0.357683 5.3:-12992309 None:intergenic
ACAATTGCGGTTACACAGTT+TGG 0.365361 5.3:+12991986 MS.gene68072:CDS
GGCGGAAACTGCACGGCAAC+GGG 0.381206 5.3:+12992342 MS.gene68072:CDS
GAACTTGGTTTACACGTATC+AGG 0.390219 5.3:-12992516 None:intergenic
TATAGTTATGCTTATGATGA+TGG 0.395195 5.3:+12992576 MS.gene68072:CDS
GAATTCACCCTCAACGGTTC+CGG 0.396695 5.3:+12992240 MS.gene68072:CDS
CCGGCGCCGGATAAAATTCC+CGG 0.403457 5.3:-12992009 None:intergenic
ATCGAACCACACGGTGAAAC+CGG 0.404042 5.3:+12992318 MS.gene68072:CDS
CGGTGGTATAGCGTTTCTTC+CGG 0.406204 5.3:-12992205 None:intergenic
TTCCTGGTCTGGCCGTCTAT+GGG 0.409304 5.3:+12992101 MS.gene68072:CDS
TGCCGTGCAGTTTCCGCCGC+CGG 0.412387 5.3:-12992337 None:intergenic
GGTGGAGAATGGTTCTGTTC+CGG 0.429649 5.3:-12992028 None:intergenic
GTGAATTCTGCTAATGTTGC+CGG 0.433552 5.3:-12992225 None:intergenic
GCCGTACCACCTTCCTGGTC+TGG 0.449891 5.3:+12992090 MS.gene68072:CDS
GGCGAGAGCGTTGGACTCGC+CGG 0.450480 5.3:-12992070 None:intergenic
AAAAGCGCGTGTGAAGCGTT+TGG 0.451521 5.3:+12992456 MS.gene68072:CDS
AAATTACCTTGTGGAGGGTG+TGG 0.467285 5.3:-12992649 None:intergenic
GTGCCAGCTACGGACGTGTC+TGG 0.474379 5.3:+12993299 MS.gene68072:CDS
AAGTCCTCCGGAACCGTTGA+GGG 0.477120 5.3:-12992247 None:intergenic
ACAAAGCCGGTGGTGGAGAA+TGG 0.482809 5.3:-12992039 None:intergenic
GGAAAATTCTCATGCATCAC+CGG 0.483024 5.3:+12992153 MS.gene68072:CDS
CGGCGGAAACTGCACGGCAA+CGG 0.484219 5.3:+12992341 MS.gene68072:CDS
CTTTGGTGGTTGAACGGTTG+TGG 0.486228 5.3:+12993387 MS.gene68072:CDS
TACTCAATTTGCCACCACTT+TGG 0.487012 5.3:+12991691 MS.gene68072:CDS
ACACCAGACACGTCCGTAGC+TGG 0.488879 5.3:-12993302 None:intergenic
TAGGAGTTGGTAGAGAATAA+GGG 0.493076 5.3:-12991717 None:intergenic
GAATGGTTCTGTTCCGGCGC+CGG 0.508113 5.3:-12992022 None:intergenic
TTGGAGAACAAAGCCGGTGG+TGG 0.508758 5.3:-12992046 None:intergenic
TTGCGGTTACACAGTTTGGC+CGG 0.509361 5.3:+12991990 MS.gene68072:CDS
AAAATATCCTGTGCCAGCTA+CGG 0.516556 5.3:+12993289 MS.gene68072:CDS
TGCGGTTACACAGTTTGGCC+GGG 0.517234 5.3:+12991991 MS.gene68072:CDS
GCGAGAGCGTTGGACTCGCC+GGG 0.518695 5.3:-12992069 None:intergenic
GTTTCTTCCGGCACATTCGA+TGG 0.519305 5.3:-12992193 None:intergenic
TTCACAATTGTGAACAATTG+CGG 0.527708 5.3:+12991973 MS.gene68072:CDS
TCCCCATAGACGGCCAGACC+AGG 0.532075 5.3:-12992103 None:intergenic
CCGCCGCCGGTTTCACCGTG+TGG 0.538076 5.3:-12992324 None:intergenic
AATTACCTTGTGGAGGGTGT+GGG 0.540652 5.3:-12992648 None:intergenic
CGTGTGGTTCGATCAATATC+GGG 0.545709 5.3:-12992308 None:intergenic
CTTGAACGCGGCGTGTCCGA+CGG 0.547717 5.3:+12992377 MS.gene68072:CDS
GAGCGTTGGACTCGCCGGGT+TGG 0.550394 5.3:-12992065 None:intergenic
TCCGGCACATTCGATGGTGG+AGG 0.550974 5.3:-12992187 None:intergenic
AATGCAGTTCAATCAAATCT+GGG 0.551133 5.3:+12993261 MS.gene68072:intron
TCCTCCACCATCGAATGTGC+CGG 0.552071 5.3:+12992186 MS.gene68072:CDS
AAGCGCGTGTGAAGCGTTTG+GGG 0.554522 5.3:+12992458 MS.gene68072:CDS
GATCCGCAATATTGCTGCAG+TGG 0.557457 5.3:+12992480 MS.gene68072:CDS
GTCGAAGAAATCAAGTCCTC+CGG 0.560323 5.3:-12992259 None:intergenic
GACACGTCCGTAGCTGGCAC+AGG 0.563395 5.3:-12993296 None:intergenic
TTCGACGTTAGTCTCGTCGA+TGG 0.567525 5.3:+12992276 MS.gene68072:CDS
GCCGGGTTGGAGAACAAAGC+CGG 0.572484 5.3:-12992052 None:intergenic
ACAGAACCATTCTCCACCAC+CGG 0.573244 5.3:+12992033 MS.gene68072:CDS
TGAAGGCTTTGGTGGTTGAA+CGG 0.573340 5.3:+12993381 MS.gene68072:CDS
AGGTGGTACGGCGAGAGCGT+TGG 0.574498 5.3:-12992079 None:intergenic
GCCAGACCAGGAAGGTGGTA+CGG 0.576483 5.3:-12992091 None:intergenic
AAGTGAAAATTACCTTGTGG+AGG 0.581305 5.3:-12992655 None:intergenic
ACCGGCTTTGTTCTCCAACC+CGG 0.581948 5.3:+12992051 MS.gene68072:CDS
CTCATCACTTTCAACTCCGT+CGG 0.583041 5.3:-12992393 None:intergenic
GAGTGTTACCTATTAGGAGT+TGG 0.590111 5.3:-12991730 None:intergenic
AGTGTGTTCTTCCCCATAGA+CGG 0.590774 5.3:-12992113 None:intergenic
ATTACCTTGTGGAGGGTGTG+GGG 0.591516 5.3:-12992647 None:intergenic
AACCGGCGGCGGAAACTGCA+CGG 0.591968 5.3:+12992335 MS.gene68072:CDS
AGTAGTAGTAATAGCAGCGG+AGG 0.599344 5.3:+12992414 MS.gene68072:CDS
CAGCGGAGGTGAAGAGAGCG+TGG 0.599856 5.3:+12992428 MS.gene68072:CDS
TCCTGGTCTGGCCGTCTATG+GGG 0.605231 5.3:+12992102 MS.gene68072:CDS
CCACACGGTGAAACCGGCGG+CGG 0.608059 5.3:+12992324 MS.gene68072:CDS
CACTGCTCTCAAGACCCCAC+CGG 0.608797 5.3:+12992132 MS.gene68072:CDS
TTAGCAGAATTCACCCTCAA+CGG 0.609111 5.3:+12992234 MS.gene68072:CDS
CGTGTCTGGTGTTCGACATG+TGG 0.609923 5.3:+12993313 MS.gene68072:CDS
GCGCCACTGCAGCAATATTG+CGG 0.611330 5.3:-12992483 None:intergenic
AGTGAAAATTACCTTGTGGA+GGG 0.612016 5.3:-12992654 None:intergenic
GAGAATAAGGGCAACCAAAG+TGG 0.615615 5.3:-12991705 None:intergenic
CAAGTCCTCCGGAACCGTTG+AGG 0.616426 5.3:-12992248 None:intergenic
TTCACCCTCAACGGTTCCGG+AGG 0.618797 5.3:+12992243 MS.gene68072:CDS
ACACGCCGCGTTCAAGTCGA+CGG 0.623825 5.3:-12992370 None:intergenic
CGGAAGAAACGCTATACCAC+CGG 0.628933 5.3:+12992206 MS.gene68072:CDS
CACCAAAGCCTTCAAAACAA+AGG 0.629976 5.3:-12993372 None:intergenic
TCTTCCGGCACATTCGATGG+TGG 0.630194 5.3:-12992190 None:intergenic
TTTGCCCCACACCCTCCACA+AGG 0.630850 5.3:+12992643 MS.gene68072:CDS
GGTGGAGGAATCACAGTCGC+CGG 0.634571 5.3:-12992172 None:intergenic
AGTTCAATCAAATCTGGGAA+CGG 0.635581 5.3:+12993266 MS.gene68072:intron
CATAGACGGCCAGACCAGGA+AGG 0.638136 5.3:-12992099 None:intergenic
ATGAGTAGTAGTAATAGCAG+CGG 0.645467 5.3:+12992411 MS.gene68072:CDS
GAACCACACGGTGAAACCGG+CGG 0.655360 5.3:+12992321 MS.gene68072:CDS
AATAAGGGCAACCAAAGTGG+TGG 0.656806 5.3:-12991702 None:intergenic
TTGCTCCGTCGACTTGAACG+CGG 0.663114 5.3:+12992365 MS.gene68072:CDS
CCGATATTGATCGAACCACA+CGG 0.663725 5.3:+12992309 MS.gene68072:CDS
AGACGGCCAGACCAGGAAGG+TGG 0.664276 5.3:-12992096 None:intergenic
TAAGCATAACTATAAGCGCG+TGG 0.672663 5.3:-12992567 None:intergenic
AATTCTGCTAATGTTGCCGG+TGG 0.685668 5.3:-12992222 None:intergenic
GGGTTGGAGAACAAAGCCGG+TGG 0.695061 5.3:-12992049 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAATTAAATTAAAAAA+AGG - chr5.3:12992970-12992989 None:intergenic 0.0%
!! TTTAACATGTTAATTATTAC+AGG + chr5.3:12991934-12991953 MS.gene68072:intron 15.0%
!!! TCTTTTACTAAAAAAAATTC+TGG - chr5.3:12992763-12992782 None:intergenic 15.0%
!! GTTCATAACAAAAAAAAATC+TGG - chr5.3:12993110-12993129 None:intergenic 20.0%
!!! CTTTTCAAAATGAATGTAAT+TGG + chr5.3:12992725-12992744 MS.gene68072:intron 20.0%
! AAAAAGTGAAAATTACCTTG+TGG - chr5.3:12992661-12992680 None:intergenic 25.0%
! GTGATAAGTTATTGAAGAAA+AGG - chr5.3:12992694-12992713 None:intergenic 25.0%
! GTTATTGAAGAAAAGGTAAA+CGG - chr5.3:12992687-12992706 None:intergenic 25.0%
! TAACAAAAAAAAATCTGGCA+TGG - chr5.3:12993105-12993124 None:intergenic 25.0%
! TATAGTTATGCTTATGATGA+TGG + chr5.3:12992576-12992595 MS.gene68072:CDS 25.0%
! TTTGTGAATCAACTAATGAT+TGG + chr5.3:12992879-12992898 MS.gene68072:intron 25.0%
AAAAAAAAATCTGGCATGGA+CGG - chr5.3:12993101-12993120 None:intergenic 30.0%
AAAAAACTAAACTGCATGTC+TGG - chr5.3:12991861-12991880 None:intergenic 30.0%
AATGCAGTTCAATCAAATCT+GGG + chr5.3:12993261-12993280 MS.gene68072:intron 30.0%
ACTACGAAAATCGAATAACT+TGG - chr5.3:12991759-12991778 None:intergenic 30.0%
ATAGAGAAAGTTGAATGTCA+AGG + chr5.3:12992991-12993010 MS.gene68072:intron 30.0%
CACAAAAACTAACTCATACA+CGG - chr5.3:12993167-12993186 None:intergenic 30.0%
TTCACAATTGTGAACAATTG+CGG + chr5.3:12991973-12991992 MS.gene68072:CDS 30.0%
!! TACACGGAATGTTTTATTGA+TGG - chr5.3:12993151-12993170 None:intergenic 30.0%
!! TGTTTTATTGATGGTACATC+TGG - chr5.3:12993142-12993161 None:intergenic 30.0%
!!! GTTAAAACGTGTTTTGAGAT+AGG + chr5.3:12992919-12992938 MS.gene68072:intron 30.0%
!!! TGTTATTGCCTTTGTTTTGA+AGG + chr5.3:12993364-12993383 MS.gene68072:CDS 30.0%
AAGTGAAAATTACCTTGTGG+AGG - chr5.3:12992658-12992677 None:intergenic 35.0%
ACGAAAATCGAATAACTTGG+CGG - chr5.3:12991756-12991775 None:intergenic 35.0%
ACTCTTAAAAAACTGCGAGT+AGG - chr5.3:12992541-12992560 None:intergenic 35.0%
AGAAAGTTGAATGTCAAGGA+GGG + chr5.3:12992995-12993014 MS.gene68072:intron 35.0%
AGTGAAAATTACCTTGTGGA+GGG - chr5.3:12992657-12992676 None:intergenic 35.0%
AGTTCAATCAAATCTGGGAA+CGG + chr5.3:12993266-12993285 MS.gene68072:intron 35.0%
ATGAGTAGTAGTAATAGCAG+CGG + chr5.3:12992411-12992430 MS.gene68072:CDS 35.0%
ATTCTCTACCAACTCCTAAT+AGG + chr5.3:12991722-12991741 MS.gene68072:CDS 35.0%
GAATGCAGTTCAATCAAATC+TGG + chr5.3:12993260-12993279 MS.gene68072:intron 35.0%
!! TAGGAGTTGGTAGAGAATAA+GGG - chr5.3:12991720-12991739 None:intergenic 35.0%
!! TTAGGAGTTGGTAGAGAATA+AGG - chr5.3:12991721-12991740 None:intergenic 35.0%
AAAAACTGCGAGTAGGAACT+TGG - chr5.3:12992534-12992553 None:intergenic 40.0%
AAAATATCCTGTGCCAGCTA+CGG + chr5.3:12993289-12993308 MS.gene68072:CDS 40.0%
ACAATTGCGGTTACACAGTT+TGG + chr5.3:12991986-12992005 MS.gene68072:CDS 40.0%
CAACGTGACAACCATATCTA+AGG - chr5.3:12993050-12993069 None:intergenic 40.0%
CACCAAAGCCTTCAAAACAA+AGG - chr5.3:12993375-12993394 None:intergenic 40.0%
CGAAAATCGAATAACTTGGC+GGG - chr5.3:12991755-12991774 None:intergenic 40.0%
GAAAGTTGAATGTCAAGGAG+GGG + chr5.3:12992996-12993015 MS.gene68072:intron 40.0%
GAACTTGGTTTACACGTATC+AGG - chr5.3:12992519-12992538 None:intergenic 40.0%
GAGAAAGTTGAATGTCAAGG+AGG + chr5.3:12992994-12993013 MS.gene68072:intron 40.0%
GAGTGTTACCTATTAGGAGT+TGG - chr5.3:12991733-12991752 None:intergenic 40.0%
GGAAAATTCTCATGCATCAC+CGG + chr5.3:12992153-12992172 MS.gene68072:CDS 40.0%
GTGAATTCTGCTAATGTTGC+CGG - chr5.3:12992228-12992247 None:intergenic 40.0%
GTGTGGTATCACCTTAGATA+TGG + chr5.3:12993036-12993055 MS.gene68072:intron 40.0%
TAAGCATAACTATAAGCGCG+TGG - chr5.3:12992570-12992589 None:intergenic 40.0%
TACTCAATTTGCCACCACTT+TGG + chr5.3:12991691-12991710 MS.gene68072:CDS 40.0%
TGCATGATCTAGCGTGATTA+TGG + chr5.3:12991814-12991833 MS.gene68072:intron 40.0%
TTAGCAGAATTCACCCTCAA+CGG + chr5.3:12992234-12992253 MS.gene68072:CDS 40.0%
! ATGCATGAGAATTTTCCGGT+GGG - chr5.3:12992150-12992169 None:intergenic 40.0%
! GGTGATGCATGAGAATTTTC+CGG - chr5.3:12992154-12992173 None:intergenic 40.0%
! TATTGATGGTACATCTGGCA+TGG - chr5.3:12993137-12993156 None:intergenic 40.0%
!!! CTTTGTTTTGAAGGCTTTGG+TGG + chr5.3:12993373-12993392 MS.gene68072:CDS 40.0%
!!! TGCCTTTGTTTTGAAGGCTT+TGG + chr5.3:12993370-12993389 MS.gene68072:CDS 40.0%
AAATTACCTTGTGGAGGGTG+TGG - chr5.3:12992652-12992671 None:intergenic 45.0%
AATAAGGGCAACCAAAGTGG+TGG - chr5.3:12991705-12991724 None:intergenic 45.0%
AATTCTGCTAATGTTGCCGG+TGG - chr5.3:12992225-12992244 None:intergenic 45.0%
AGTAGTAGTAATAGCAGCGG+AGG + chr5.3:12992414-12992433 MS.gene68072:CDS 45.0%
AGTGTGTTCTTCCCCATAGA+CGG - chr5.3:12992116-12992135 None:intergenic 45.0%
CCGATATTGATCGAACCACA+CGG + chr5.3:12992309-12992328 MS.gene68072:CDS 45.0%
CCGTGTGGTTCGATCAATAT+CGG - chr5.3:12992312-12992331 None:intergenic 45.0%
CGTGTGGTTCGATCAATATC+GGG - chr5.3:12992311-12992330 None:intergenic 45.0%
CTCATCACTTTCAACTCCGT+CGG - chr5.3:12992396-12992415 None:intergenic 45.0%
GAGAATAAGGGCAACCAAAG+TGG - chr5.3:12991708-12991727 None:intergenic 45.0%
GTCGAAGAAATCAAGTCCTC+CGG - chr5.3:12992262-12992281 None:intergenic 45.0%
TTGTGTAGCTAGCTACTAGC+TGG + chr5.3:12993182-12993201 MS.gene68072:intron 45.0%
! AACTGTTGGCTGTTATGGTG+TGG + chr5.3:12993019-12993038 MS.gene68072:intron 45.0%
! AATTACCTTGTGGAGGGTGT+GGG - chr5.3:12992651-12992670 None:intergenic 45.0%
! GATGCATGAGAATTTTCCGG+TGG - chr5.3:12992151-12992170 None:intergenic 45.0%
! GTTTGGCCGGGAATTTTATC+CGG + chr5.3:12992003-12992022 MS.gene68072:CDS 45.0%
! TGAAGGCTTTGGTGGTTGAA+CGG + chr5.3:12993381-12993400 MS.gene68072:CDS 45.0%
! TGCATGAGAATTTTCCGGTG+GGG - chr5.3:12992149-12992168 None:intergenic 45.0%
ACAGAACCATTCTCCACCAC+CGG + chr5.3:12992033-12992052 MS.gene68072:CDS 50.0%
ATCGAACCACACGGTGAAAC+CGG + chr5.3:12992318-12992337 MS.gene68072:CDS 50.0%
ATGTCAAGGAGGGGAACTGT+TGG + chr5.3:12993005-12993024 MS.gene68072:intron 50.0%
CGGAAGAAACGCTATACCAC+CGG + chr5.3:12992206-12992225 MS.gene68072:CDS 50.0%
CGGTGGTATAGCGTTTCTTC+CGG - chr5.3:12992208-12992227 None:intergenic 50.0%
GAATTCACCCTCAACGGTTC+CGG + chr5.3:12992240-12992259 MS.gene68072:CDS 50.0%
GATCCGCAATATTGCTGCAG+TGG + chr5.3:12992480-12992499 MS.gene68072:CDS 50.0%
GTTTCTTCCGGCACATTCGA+TGG - chr5.3:12992196-12992215 None:intergenic 50.0%
TATTGCTGCAGTGGCGCTTA+TGG + chr5.3:12992489-12992508 MS.gene68072:CDS 50.0%
TTGCGGTTACACAGTTTGGC+CGG + chr5.3:12991990-12992009 MS.gene68072:CDS 50.0%
! AAAAGCGCGTGTGAAGCGTT+TGG + chr5.3:12992456-12992475 MS.gene68072:CDS 50.0%
! AAAGCGCGTGTGAAGCGTTT+GGG + chr5.3:12992457-12992476 MS.gene68072:CDS 50.0%
! AGGGGAACTGTTGGCTGTTA+TGG + chr5.3:12993014-12993033 MS.gene68072:intron 50.0%
! ATTACCTTGTGGAGGGTGTG+GGG - chr5.3:12992650-12992669 None:intergenic 50.0%
! CTTTGGTGGTTGAACGGTTG+TGG + chr5.3:12993387-12993406 MS.gene68072:CDS 50.0%
! GGTGGAGAATGGTTCTGTTC+CGG - chr5.3:12992031-12992050 None:intergenic 50.0%
! TTCGACGTTAGTCTCGTCGA+TGG + chr5.3:12992276-12992295 MS.gene68072:CDS 50.0%
!! TGGCGGGAGTGTTACCTATT+AGG - chr5.3:12991739-12991758 None:intergenic 50.0%
AAGTCCTCCGGAACCGTTGA+GGG - chr5.3:12992250-12992269 None:intergenic 55.0%
CGTGTCTGGTGTTCGACATG+TGG + chr5.3:12993313-12993332 MS.gene68072:CDS 55.0%
GCGCCACTGCAGCAATATTG+CGG - chr5.3:12992486-12992505 None:intergenic 55.0%
GGGTGTGGGGCAAAAAGTGA+TGG - chr5.3:12992637-12992656 None:intergenic 55.0%
TCCTCCACCATCGAATGTGC+CGG + chr5.3:12992186-12992205 MS.gene68072:CDS 55.0%
TCTTCCGGCACATTCGATGG+TGG - chr5.3:12992193-12992212 None:intergenic 55.0%
TGCGGTTACACAGTTTGGCC+GGG + chr5.3:12991991-12992010 MS.gene68072:CDS 55.0%
TTCCTGGTCTGGCCGTCTAT+GGG + chr5.3:12992101-12992120 MS.gene68072:CDS 55.0%
TTGGAGAACAAAGCCGGTGG+TGG - chr5.3:12992049-12992068 None:intergenic 55.0%
! AAGCGCGTGTGAAGCGTTTG+GGG + chr5.3:12992458-12992477 MS.gene68072:CDS 55.0%
!! ACAAAGCCGGTGGTGGAGAA+TGG - chr5.3:12992042-12992061 None:intergenic 55.0%
!! ACCGGCTTTGTTCTCCAACC+CGG + chr5.3:12992051-12992070 MS.gene68072:CDS 55.0%
!! TTGCTCCGTCGACTTGAACG+CGG + chr5.3:12992365-12992384 MS.gene68072:CDS 55.0%
ACACCAGACACGTCCGTAGC+TGG - chr5.3:12993305-12993324 None:intergenic 60.0%
ACACGCCGCGTTCAAGTCGA+CGG - chr5.3:12992373-12992392 None:intergenic 60.0%
CAAGTCCTCCGGAACCGTTG+AGG - chr5.3:12992251-12992270 None:intergenic 60.0%
CACTGCTCTCAAGACCCCAC+CGG + chr5.3:12992132-12992151 MS.gene68072:CDS 60.0%
CATAGACGGCCAGACCAGGA+AGG - chr5.3:12992102-12992121 None:intergenic 60.0%
CTTCCTGGTCTGGCCGTCTA+TGG + chr5.3:12992100-12992119 MS.gene68072:CDS 60.0%
GAACCACACGGTGAAACCGG+CGG + chr5.3:12992321-12992340 MS.gene68072:CDS 60.0%
GCCAGACCAGGAAGGTGGTA+CGG - chr5.3:12992094-12992113 None:intergenic 60.0%
GGGTTGGAGAACAAAGCCGG+TGG - chr5.3:12992052-12992071 None:intergenic 60.0%
GGTGGAGGAATCACAGTCGC+CGG - chr5.3:12992175-12992194 None:intergenic 60.0%
TCCTGGTCTGGCCGTCTATG+GGG + chr5.3:12992102-12992121 MS.gene68072:CDS 60.0%
TTCACCCTCAACGGTTCCGG+AGG + chr5.3:12992243-12992262 MS.gene68072:CDS 60.0%
TTTGCCCCACACCCTCCACA+AGG + chr5.3:12992643-12992662 MS.gene68072:CDS 60.0%
! CCGGCGCCGGATAAAATTCC+CGG - chr5.3:12992012-12992031 None:intergenic 60.0%
! CCGGGAATTTTATCCGGCGC+CGG + chr5.3:12992009-12992028 MS.gene68072:CDS 60.0%
! GAATGGTTCTGTTCCGGCGC+CGG - chr5.3:12992025-12992044 None:intergenic 60.0%
! GCCGGGTTGGAGAACAAAGC+CGG - chr5.3:12992055-12992074 None:intergenic 60.0%
! TCCGGCACATTCGATGGTGG+AGG - chr5.3:12992190-12992209 None:intergenic 60.0%
AACCGGCGGCGGAAACTGCA+CGG + chr5.3:12992335-12992354 MS.gene68072:CDS 65.0%
AGACGGCCAGACCAGGAAGG+TGG - chr5.3:12992099-12992118 None:intergenic 65.0%
CAGCGGAGGTGAAGAGAGCG+TGG + chr5.3:12992428-12992447 MS.gene68072:CDS 65.0%
CGGCGGAAACTGCACGGCAA+CGG + chr5.3:12992341-12992360 MS.gene68072:CDS 65.0%
CTCTCGCCGTACCACCTTCC+TGG + chr5.3:12992085-12992104 MS.gene68072:CDS 65.0%
CTTGAACGCGGCGTGTCCGA+CGG + chr5.3:12992377-12992396 MS.gene68072:CDS 65.0%
GACACGTCCGTAGCTGGCAC+AGG - chr5.3:12993299-12993318 None:intergenic 65.0%
GCCGTACCACCTTCCTGGTC+TGG + chr5.3:12992090-12992109 MS.gene68072:CDS 65.0%
GGCGGAAACTGCACGGCAAC+GGG + chr5.3:12992342-12992361 MS.gene68072:CDS 65.0%
GTGCCAGCTACGGACGTGTC+TGG + chr5.3:12993299-12993318 MS.gene68072:CDS 65.0%
TCCCCATAGACGGCCAGACC+AGG - chr5.3:12992106-12992125 None:intergenic 65.0%
! AGGTGGTACGGCGAGAGCGT+TGG - chr5.3:12992082-12992101 None:intergenic 65.0%
CCACACGGTGAAACCGGCGG+CGG + chr5.3:12992324-12992343 MS.gene68072:CDS 70.0%
TGCCGTGCAGTTTCCGCCGC+CGG - chr5.3:12992340-12992359 None:intergenic 70.0%
!! GAGCGTTGGACTCGCCGGGT+TGG - chr5.3:12992068-12992087 None:intergenic 70.0%
!! GCGAGAGCGTTGGACTCGCC+GGG - chr5.3:12992072-12992091 None:intergenic 70.0%
!! GGCGAGAGCGTTGGACTCGC+CGG - chr5.3:12992073-12992092 None:intergenic 70.0%
!! CCGCCGCCGGTTTCACCGTG+TGG - chr5.3:12992327-12992346 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 12991680 12993412 12991680 ID=MS.gene68072
chr5.3 mRNA 12991680 12993412 12991680 ID=MS.gene68072.t1;Parent=MS.gene68072
chr5.3 exon 12991680 12991743 12991680 ID=MS.gene68072.t1.exon1;Parent=MS.gene68072.t1
chr5.3 CDS 12991680 12991743 12991680 ID=cds.MS.gene68072.t1;Parent=MS.gene68072.t1
chr5.3 exon 12991956 12992664 12991956 ID=MS.gene68072.t1.exon2;Parent=MS.gene68072.t1
chr5.3 CDS 12991956 12992664 12991956 ID=cds.MS.gene68072.t1;Parent=MS.gene68072.t1
chr5.3 exon 12993268 12993412 12993268 ID=MS.gene68072.t1.exon3;Parent=MS.gene68072.t1
chr5.3 CDS 12993268 12993412 12993268 ID=cds.MS.gene68072.t1;Parent=MS.gene68072.t1
Gene Sequence

>MS.gene68072

ATGGCTTCTTCTACTCAATTTGCCACCACTTTGGTTGCCCTTATTCTCTACCAACTCCTAATAGGTAACACTCCCGCCAAGTTATTCGATTTTCGTAGTGCATGTTTGAATTCACGATGAATTTGTTAGAATCATGCATGATCTAGCGTGATTATGGCAAATTTATCTAACAATGATGCCAGACATGCAGTTTAGTTTTTTTTGTTGTTTTTAATGTTCACATAAAAGTTTGTGTCTTAAGTTGCTTTCTTGTTTTTAACATGTTAATTATTACAGGTTCATATTCATCAACATTCACAATTGTGAACAATTGCGGTTACACAGTTTGGCCGGGAATTTTATCCGGCGCCGGAACAGAACCATTCTCCACCACCGGCTTTGTTCTCCAACCCGGCGAGTCCAACGCTCTCGCCGTACCACCTTCCTGGTCTGGCCGTCTATGGGGAAGAACACACTGCTCTCAAGACCCCACCGGAAAATTCTCATGCATCACCGGCGACTGTGATTCCTCCACCATCGAATGTGCCGGAAGAAACGCTATACCACCGGCAACATTAGCAGAATTCACCCTCAACGGTTCCGGAGGACTTGATTTCTTCGACGTTAGTCTCGTCGATGGTTACAATCTCCCGATATTGATCGAACCACACGGTGAAACCGGCGGCGGAAACTGCACGGCAACGGGTTGCTCCGTCGACTTGAACGCGGCGTGTCCGACGGAGTTGAAAGTGATGAGTAGTAGTAATAGCAGCGGAGGTGAAGAGAGCGTGGCGTGTAAAAGCGCGTGTGAAGCGTTTGGGGATCCGCAATATTGCTGCAGTGGCGCTTATGGTTCGCCTGATACGTGTAAACCAAGTTCCTACTCGCAGTTTTTTAAGAGTGCGTGTCCACGCGCTTATAGTTATGCTTATGATGATGGAACTAGCACTTTCACTTGTGCTTCTGCTGATTATACCATCACTTTTTGCCCCACACCCTCCACAAGGTAATTTTCACTTTTTTTACCGTTTACCTTTTCTTCAATAACTTATCACAATTTTCTTTTCTTTTCAAAATGAATGTAATTGGTTGTCAAAAAGACCAGAATTTTTTTTAGTAAAAGATGCAAAGATATTTGATATGTTAGAATACGAGATTCGAACATTTGATTTTAGTGTGAATGAATTTTGTCGCTAAATATTTTAGAGTAAACAAAATAATTTGTGAATCAACTAATGATTGGAATTGCTATTATTAGTTGTTAAAACGTGTTTTGAGATAGGAATATTGCTTTGAATTTTCATATCACCTTTTTTTAATTTAATTATTAAGATAGAGAAAGTTGAATGTCAAGGAGGGGAACTGTTGGCTGTTATGGTGTGGTATCACCTTAGATATGGTTGTCACGTTGCATTTTTTCTCTGTTATTTATTTATGCACCGTCCATGCCAGATTTTTTTTTGTTATGAACTATGCCATGCCAGATGTACCATCAATAAAACATTCCGTGTATGAGTTAGTTTTTGTGTAGCTAGCTACTAGCTGGTTTTGTTTTGTGTTGAAGTTTGAGTGCAACATTTTGAAAACTCTTGTTTGAATGTGAATGCAGTTCAATCAAATCTGGGAACGGAAAATATCCTGTGCCAGCTACGGACGTGTCTGGTGTTCGACATGTGGATCAGAGAGTCAACAACGTTGTAACGATTGTTATTGCCTTTGTTTTGAAGGCTTTGGTGGTTGAACGGTTGTGGTAA
Protein sequence

>MS.gene68072.t1

MASSTQFATTLVALILYQLLIGSYSSTFTIVNNCGYTVWPGILSGAGTEPFSTTGFVLQPGESNALAVPPSWSGRLWGRTHCSQDPTGKFSCITGDCDSSTIECAGRNAIPPATLAEFTLNGSGGLDFFDVSLVDGYNLPILIEPHGETGGGNCTATGCSVDLNAACPTELKVMSSSNSSGGEESVACKSACEAFGDPQYCCSGAYGSPDTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCASADYTITFCPTPSTSSIKSGNGKYPVPATDVSGVRHVDQRVNNVVTIVIAFVLKALVVERLW