AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59476

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59476.t1 MTR_5g022310 94.737 304 12 2 1 304 1 300 0.0 582
MS.gene59476.t1 MTR_5g022310 95.686 255 10 1 1 255 1 254 4.93e-179 494
MS.gene59476.t1 MTR_8g037890 72.671 322 59 6 1 299 1 316 9.26e-159 445
MS.gene59476.t1 MTR_8g056820 80.385 260 46 1 1 260 1 255 2.77e-150 422
MS.gene59476.t1 MTR_8g075550 68.092 304 88 5 1 298 1 301 3.18e-138 393
MS.gene59476.t1 MTR_1g025420 67.589 253 76 4 14 265 11 258 2.63e-118 343
MS.gene59476.t1 MTR_1g025420 67.611 247 75 3 14 259 11 253 4.96e-117 338
MS.gene59476.t1 MTR_3g114030 70.293 239 66 3 20 257 1 235 1.11e-116 336
MS.gene59476.t1 MTR_4g073730 59.851 269 98 4 11 272 5 270 2.77e-106 311
MS.gene59476.t1 MTR_4g073730 59.851 269 98 4 11 272 5 270 4.10e-106 312
MS.gene59476.t1 MTR_4g073720 57.025 242 89 4 14 251 9 239 5.93e-90 271
MS.gene59476.t1 MTR_5g022350 53.704 270 108 7 1 267 4 259 2.00e-88 267
MS.gene59476.t1 MTR_8g075510 53.937 254 100 7 3 255 10 247 1.48e-83 252
MS.gene59476.t1 MTR_7g102380 50.800 250 112 5 4 252 2 241 1.05e-82 249
MS.gene59476.t1 MTR_8g075510 54.000 250 98 7 3 251 10 243 3.73e-82 251
MS.gene59476.t1 MTR_2g069660 52.049 244 109 4 11 252 6 243 1.23e-78 239
MS.gene59476.t1 MTR_1g062590 49.398 249 116 5 7 252 1 242 3.00e-78 238
MS.gene59476.t1 MTR_2g068030 51.092 229 103 5 25 251 22 243 3.64e-78 238
MS.gene59476.t1 MTR_8g036215 49.388 245 116 4 7 251 1 237 2.17e-77 236
MS.gene59476.t1 MTR_2g068655 50.000 232 109 3 22 252 27 252 5.21e-77 238
MS.gene59476.t1 MTR_1g062630 51.190 252 109 6 7 253 1 243 1.95e-75 232
MS.gene59476.t1 MTR_1g062660 50.617 243 108 6 12 253 12 243 2.54e-75 231
MS.gene59476.t1 MTR_6g079580 48.790 248 113 5 11 252 1 240 5.38e-75 230
MS.gene59476.t1 MTR_2g067980 46.899 258 124 5 10 261 6 256 5.44e-75 248
MS.gene59476.t1 MTR_1g062340 50.000 242 109 6 13 253 13 243 6.90e-75 230
MS.gene59476.t1 MTR_8g107140 50.209 239 111 4 14 252 15 245 2.32e-74 229
MS.gene59476.t1 MTR_1g062350 48.810 252 118 6 3 253 2 243 1.94e-73 226
MS.gene59476.t1 MTR_3g111620 53.043 230 99 5 28 256 29 250 4.26e-73 225
MS.gene59476.t1 MTR_1g062370 48.016 252 120 6 3 253 2 243 5.93e-73 225
MS.gene59476.t1 MTR_1g021945 51.709 234 102 6 25 256 25 249 7.66e-73 224
MS.gene59476.t1 MTR_7g062610 44.444 252 131 4 5 255 2 245 1.13e-68 214
MS.gene59476.t1 MTR_5g023850 45.247 263 100 5 1 259 1 223 1.56e-68 213
MS.gene59476.t1 MTR_5g059200 46.311 244 112 7 12 251 11 239 4.65e-67 211
MS.gene59476.t1 MTR_3g068015 45.415 229 115 5 26 252 28 248 5.18e-63 199
MS.gene59476.t1 MTR_6g009480 40.551 254 144 4 1 252 4 252 3.38e-62 198
MS.gene59476.t1 MTR_1g062690 43.254 252 108 7 3 253 2 219 1.11e-57 185
MS.gene59476.t1 MTR_4g063630 41.322 242 131 8 14 251 17 251 5.51e-55 179
MS.gene59476.t1 MTR_2g067990 42.324 241 106 7 22 261 23 231 3.87e-53 187
MS.gene59476.t1 MTR_2g067970 45.789 190 93 6 69 255 3 185 9.48e-47 168
MS.gene59476.t1 MTR_5g010640 38.077 260 129 9 1 252 1 236 3.35e-46 156
MS.gene59476.t1 MTR_5g010635 39.655 232 111 8 23 252 21 225 6.87e-46 155
MS.gene59476.t1 MTR_8g096900 38.400 250 120 9 9 252 27 248 4.77e-44 150
MS.gene59476.t1 MTR_2g063150 35.160 219 130 5 27 244 35 242 5.04e-40 140
MS.gene59476.t1 MTR_5g022350 48.125 160 75 4 1 158 69 222 2.97e-39 137
MS.gene59476.t1 MTR_8g089020 48.507 134 59 3 111 244 33 156 1.28e-35 126
MS.gene59476.t1 MTR_7g076360 35.321 218 72 4 25 242 7 155 1.92e-31 115
MS.gene59476.t1 MTR_1g062390 31.783 258 150 9 1 252 1 238 5.32e-31 117
MS.gene59476.t1 MTR_8g096910 43.165 139 61 6 10 141 2 129 1.19e-26 103
MS.gene59476.t1 MTR_8g096920 41.176 136 69 5 10 143 2 128 8.91e-22 90.5
MS.gene59476.t1 MTR_3g081550 48.039 102 49 2 107 208 12 109 1.13e-21 88.6
MS.gene59476.t1 MTR_1g062640 43.333 90 48 2 79 168 60 146 1.61e-16 75.9
MS.gene59476.t1 MTR_2g063160 36.170 94 56 3 30 121 18 109 1.63e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59476.t1 AT4G36010 58.537 287 109 4 22 303 19 300 3.75e-115 334
MS.gene59476.t1 AT4G36010 58.537 287 109 4 22 303 19 300 3.75e-115 334
MS.gene59476.t1 AT1G75800 63.218 261 91 5 11 269 7 264 5.65e-113 330
MS.gene59476.t1 AT2G17860 63.563 247 84 4 11 255 10 252 2.04e-109 318
MS.gene59476.t1 AT1G20030 66.527 239 73 4 22 257 16 250 1.41e-107 315
MS.gene59476.t1 AT1G20030 66.949 236 71 4 25 257 2 233 3.02e-107 314
MS.gene59476.t1 AT4G38670 63.900 241 81 4 27 265 24 260 3.17e-104 306
MS.gene59476.t1 AT4G38670 63.900 241 81 4 27 265 24 260 3.17e-104 306
MS.gene59476.t1 AT4G38670 63.900 241 81 4 27 265 8 244 7.98e-104 304
MS.gene59476.t1 AT4G38660 53.659 287 108 6 1 273 2 277 3.79e-97 290
MS.gene59476.t1 AT4G38660 56.015 266 95 5 19 273 1 255 2.17e-96 287
MS.gene59476.t1 AT4G24180 54.412 272 104 7 1 262 6 267 2.92e-91 274
MS.gene59476.t1 AT4G24180 54.962 262 99 6 1 253 6 257 3.85e-91 272
MS.gene59476.t1 AT4G24180 54.444 270 102 7 1 262 6 262 4.07e-90 271
MS.gene59476.t1 AT4G24180 55.000 260 97 6 1 253 6 252 9.26e-90 268
MS.gene59476.t1 AT5G24620 55.328 244 106 3 11 252 9 251 1.48e-86 266
MS.gene59476.t1 AT5G24620 55.328 244 106 3 11 252 64 306 4.66e-86 266
MS.gene59476.t1 AT5G24620 55.328 244 106 3 11 252 85 327 5.63e-86 266
MS.gene59476.t1 AT5G38280 50.988 253 120 3 7 257 5 255 1.99e-84 267
MS.gene59476.t1 AT5G38280 50.988 253 120 3 7 257 5 255 2.57e-84 267
MS.gene59476.t1 AT4G24180 52.846 246 97 6 1 237 6 241 1.45e-79 244
MS.gene59476.t1 AT1G75040 52.893 242 103 5 11 252 9 239 3.46e-79 241
MS.gene59476.t1 AT1G19320 50.612 245 108 5 11 251 11 246 1.01e-78 239
MS.gene59476.t1 AT1G75030 50.407 246 113 6 11 255 9 246 1.34e-78 239
MS.gene59476.t1 AT4G24180 52.869 244 95 6 1 237 6 236 2.22e-78 241
MS.gene59476.t1 AT1G75050 49.802 253 118 6 1 253 1 244 1.10e-77 237
MS.gene59476.t1 AT1G73620 52.193 228 101 4 25 252 41 260 7.60e-76 233
MS.gene59476.t1 AT1G18250 51.739 230 103 4 23 252 19 240 2.81e-75 231
MS.gene59476.t1 AT1G18250 51.754 228 102 4 25 252 20 239 4.24e-75 230
MS.gene59476.t1 AT1G77700 47.541 244 109 7 12 251 43 271 7.77e-70 220
MS.gene59476.t1 AT1G77700 47.131 244 110 7 12 251 43 271 8.71e-70 219
MS.gene59476.t1 AT1G77700 49.339 227 100 6 25 251 88 299 2.76e-69 219
MS.gene59476.t1 AT4G36000 64.881 168 42 4 102 269 1 151 2.06e-66 207
MS.gene59476.t1 AT4G36000 64.881 168 42 4 102 269 1 151 2.06e-66 207
MS.gene59476.t1 AT5G02140 44.000 250 130 4 9 255 2 244 8.84e-65 206
MS.gene59476.t1 AT5G02140 44.130 247 128 4 9 252 2 241 9.67e-65 204
MS.gene59476.t1 AT2G28790 43.083 253 132 8 3 251 4 248 6.40e-60 192
MS.gene59476.t1 AT2G28790 43.083 253 132 8 3 251 29 273 7.21e-60 192
MS.gene59476.t1 AT4G18253 42.800 250 127 5 11 257 7 243 3.35e-59 189
MS.gene59476.t1 AT5G40020 40.598 234 131 4 21 253 23 249 2.04e-58 188
MS.gene59476.t1 AT1G70250 44.304 237 118 7 25 256 144 371 6.63e-58 199
MS.gene59476.t1 AT4G18250 39.527 296 160 9 11 301 40 321 1.52e-55 191
MS.gene59476.t1 AT4G11650 37.868 272 134 10 7 274 1 241 1.26e-48 162
MS.gene59476.t1 AT5G24620 60.833 120 47 0 133 252 15 134 1.11e-43 151
MS.gene59476.t1 AT2G24810 57.317 82 30 1 170 251 116 192 3.55e-26 102

Find 97 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTGGTCTGGCCGTCTA+TGG 0.191862 5.4:+13809023 MS.gene59476:CDS
TTAGGAGTTGGTAGAGAATA+AGG 0.252939 5.4:-13808645 None:intergenic
TGGCGGGAGTGTTACCTATT+AGG 0.288934 5.4:-13808663 None:intergenic
TATTGCTGCAGTGGCGCTTA+TGG 0.293843 5.4:+13809409 MS.gene59476:CDS
GAATGCAGTTCAATCAAATC+TGG 0.324954 5.4:+13810279 MS.gene59476:intron
CTCTCGCCGTACCACCTTCC+TGG 0.337002 5.4:+13809008 MS.gene59476:CDS
ATTCTCTACCAACTCCTAAT+AGG 0.344279 5.4:+13808649 MS.gene59476:CDS
CCGTGTGGTTCGATCAATAT+CGG 0.357683 5.4:-13809232 None:intergenic
ACAATTGCGGTTACACAGTT+TGG 0.365361 5.4:+13808909 MS.gene59476:CDS
GGCGGAAACTGCACGGCAAC+GGG 0.381206 5.4:+13809265 MS.gene59476:CDS
GAACTTGGTTTACACGTATC+AGG 0.390219 5.4:-13809436 None:intergenic
TATAGTTATGCTTATGATGA+TGG 0.395195 5.4:+13809496 MS.gene59476:CDS
GAATTCACCCTCAACGGTTC+CGG 0.396695 5.4:+13809163 MS.gene59476:CDS
CCGGCGCCGGATAAAATTCC+CGG 0.403457 5.4:-13808932 None:intergenic
ATCGAACCACACGGTGAAAC+CGG 0.404042 5.4:+13809241 MS.gene59476:CDS
CGGTGGTATAGCGTTTCTTC+CGG 0.406204 5.4:-13809128 None:intergenic
TTCCTGGTCTGGCCGTCTAT+GGG 0.409304 5.4:+13809024 MS.gene59476:CDS
TGCCGTGCAGTTTCCGCCGC+CGG 0.412387 5.4:-13809260 None:intergenic
GAATGGAAAATATCCTTTGC+CGG 0.417566 5.4:+13810302 MS.gene59476:CDS
GGTGGAGAATGGTTCTGTTC+CGG 0.429649 5.4:-13808951 None:intergenic
GTGAATTCTGCTAATGTTGC+CGG 0.433552 5.4:-13809148 None:intergenic
AAATTACCTTGTGGAGGGTG+TGG 0.445701 5.4:-13809569 None:intergenic
GCCGTACCACCTTCCTGGTC+TGG 0.449891 5.4:+13809013 MS.gene59476:CDS
GGCGAGAGCGTTGGACTCGC+CGG 0.450480 5.4:-13808993 None:intergenic
AAAAGTGCGTGTGAAGCGTT+TGG 0.466466 5.4:+13809376 MS.gene59476:CDS
CTTTGGTGGTTGAACGGTTG+TGG 0.471275 5.4:+13810406 MS.gene59476:CDS
AAGTCCTCCGGAACCGTTGA+GGG 0.477120 5.4:-13809170 None:intergenic
ACAAAGCCGGTGGTGGAGAA+TGG 0.482809 5.4:-13808962 None:intergenic
GGAAAATTCTCATGCATCAC+CGG 0.483024 5.4:+13809076 MS.gene59476:CDS
CGGCGGAAACTGCACGGCAA+CGG 0.484219 5.4:+13809264 MS.gene59476:CDS
AAAGTGCGTGTGAAGCGTTT+GGG 0.486958 5.4:+13809377 MS.gene59476:CDS
TACTCAATTTGCCACCACTT+TGG 0.487012 5.4:+13808618 MS.gene59476:CDS
TTGCCGGCTACAGACGTGTC+TGG 0.488010 5.4:+13810318 MS.gene59476:CDS
TAGGAGTTGGTAGAGAATAA+GGG 0.493076 5.4:-13808644 None:intergenic
GAATGGTTCTGTTCCGGCGC+CGG 0.508113 5.4:-13808945 None:intergenic
TTGGAGAACAAAGCCGGTGG+TGG 0.508758 5.4:-13808969 None:intergenic
TTGCGGTTACACAGTTTGGC+CGG 0.509361 5.4:+13808913 MS.gene59476:CDS
AATTACCTTGTGGAGGGTGT+GGG 0.513047 5.4:-13809568 None:intergenic
ACACGCCGCGTTTAAGTTGA+CGG 0.516248 5.4:-13809293 None:intergenic
TGCGGTTACACAGTTTGGCC+GGG 0.517234 5.4:+13808914 MS.gene59476:CDS
GCGAGAGCGTTGGACTCGCC+GGG 0.518695 5.4:-13808992 None:intergenic
GTTTCTTCCGGCACATTCGA+TGG 0.519305 5.4:-13809116 None:intergenic
TTCACAATTGTGAACAATTG+CGG 0.527708 5.4:+13808896 MS.gene59476:CDS
TCCCCATAGACGGCCAGACC+AGG 0.532075 5.4:-13809026 None:intergenic
TGATGGCTTTGGTGGTTGAA+CGG 0.537878 5.4:+13810400 MS.gene59476:CDS
CCGCCGCCGGTTTCACCGTG+TGG 0.538076 5.4:-13809247 None:intergenic
CACCAAAGCCATCAAAACAA+AGG 0.545390 5.4:-13810391 None:intergenic
CGTGTGGTTCGATCAATATC+GGG 0.545709 5.4:-13809231 None:intergenic
GAGCGTTGGACTCGCCGGGT+TGG 0.550394 5.4:-13808988 None:intergenic
TCCGGCACATTCGATGGTGG+AGG 0.550974 5.4:-13809110 None:intergenic
AATGCAGTTCAATCAAATCT+GGG 0.551133 5.4:+13810280 MS.gene59476:intron
TCCTCCACCATCGAATGTGC+CGG 0.552071 5.4:+13809109 MS.gene59476:CDS
AAGTGCGTGTGAAGCGTTTG+GGG 0.556615 5.4:+13809378 MS.gene59476:CDS
GATCCGCAATATTGCTGCAG+TGG 0.557457 5.4:+13809400 MS.gene59476:CDS
GTCGAAGAAATCAAGTCCTC+CGG 0.560323 5.4:-13809182 None:intergenic
ACACCAGACACGTCTGTAGC+CGG 0.563747 5.4:-13810321 None:intergenic
ATGAGTAGTAATAGCGGCGG+AGG 0.570006 5.4:+13809334 MS.gene59476:CDS
GCCGGGTTGGAGAACAAAGC+CGG 0.572484 5.4:-13808975 None:intergenic
ACAGAACCATTCTCCACCAC+CGG 0.573244 5.4:+13808956 MS.gene59476:CDS
AGGTGGTACGGCGAGAGCGT+TGG 0.574498 5.4:-13809002 None:intergenic
GCCAGACCAGGAAGGTGGTA+CGG 0.576483 5.4:-13809014 None:intergenic
ACCGGCTTTGTTCTCCAACC+CGG 0.581948 5.4:+13808974 MS.gene59476:CDS
CTCATCACTTTCAACTCCGT+CGG 0.583041 5.4:-13809316 None:intergenic
GAGTGTTACCTATTAGGAGT+TGG 0.590111 5.4:-13808657 None:intergenic
AGTGTGTTCTTCCCCATAGA+CGG 0.590774 5.4:-13809036 None:intergenic
AACCGGCGGCGGAAACTGCA+CGG 0.591968 5.4:+13809258 MS.gene59476:CDS
GACACGTCTGTAGCCGGCAA+AGG 0.594440 5.4:-13810315 None:intergenic
CTTAAACGCGGCGTGTCCGA+CGG 0.600103 5.4:+13809300 MS.gene59476:CDS
AAGAGTCAACAACGTTGTAA+CGG 0.600293 5.4:+13810359 MS.gene59476:CDS
ATTACCTTGTGGAGGGTGTG+GGG 0.604147 5.4:-13809567 None:intergenic
TCCTGGTCTGGCCGTCTATG+GGG 0.605231 5.4:+13809025 MS.gene59476:CDS
AGTTCAATCAAATCTGGGAA+TGG 0.606548 5.4:+13810285 MS.gene59476:intron
CCACACGGTGAAACCGGCGG+CGG 0.608059 5.4:+13809247 MS.gene59476:CDS
CACTGCTCTCAAGACCCCAC+CGG 0.608797 5.4:+13809055 MS.gene59476:CDS
TTAGCAGAATTCACCCTCAA+CGG 0.609111 5.4:+13809157 MS.gene59476:CDS
GCGCCACTGCAGCAATATTG+CGG 0.611330 5.4:-13809403 None:intergenic
CGTGTCTGGTGTTCGACATG+TGG 0.614785 5.4:+13810332 MS.gene59476:CDS
GAGAATAAGGGCAACCAAAG+TGG 0.615615 5.4:-13808632 None:intergenic
CAAGTCCTCCGGAACCGTTG+AGG 0.616426 5.4:-13809171 None:intergenic
TTCACCCTCAACGGTTCCGG+AGG 0.618797 5.4:+13809166 MS.gene59476:CDS
TTCGACGTTAGTCTCGTCGA+CGG 0.621410 5.4:+13809199 MS.gene59476:CDS
CGGCGGAGGTGAAGAGAGCG+TGG 0.621463 5.4:+13809348 MS.gene59476:CDS
CGGAAGAAACGCTATACCAC+CGG 0.628933 5.4:+13809129 MS.gene59476:CDS
TCTTCCGGCACATTCGATGG+TGG 0.630194 5.4:-13809113 None:intergenic
TTTGCCCCACACCCTCCACA+AGG 0.630850 5.4:+13809563 MS.gene59476:CDS
GGTGGAGGAATCACAGTCGC+CGG 0.634571 5.4:-13809095 None:intergenic
CATAGACGGCCAGACCAGGA+AGG 0.638136 5.4:-13809022 None:intergenic
GAACCACACGGTGAAACCGG+CGG 0.655360 5.4:+13809244 MS.gene59476:CDS
AATAAGGGCAACCAAAGTGG+TGG 0.656806 5.4:-13808629 None:intergenic
CCGATATTGATCGAACCACA+CGG 0.663725 5.4:+13809232 MS.gene59476:CDS
AGACGGCCAGACCAGGAAGG+TGG 0.664276 5.4:-13809019 None:intergenic
TAAGCATAACTATAAGCGCG+TGG 0.672663 5.4:-13809487 None:intergenic
AATTCTGCTAATGTTGCCGG+TGG 0.685668 5.4:-13809145 None:intergenic
GGGTTGGAGAACAAAGCCGG+TGG 0.695061 5.4:-13808972 None:intergenic
AAAGTGATGAGTAGTAATAG+CGG 0.708274 5.4:+13809328 MS.gene59476:CDS
TTGCTCCGTCAACTTAAACG+CGG 0.730859 5.4:+13809288 MS.gene59476:CDS
GTGATGAGTAGTAATAGCGG+CGG 0.734866 5.4:+13809331 MS.gene59476:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTCAAAAAGTATAAT+TGG + chr5.4:13809643-13809662 MS.gene59476:intron 10.0%
!! TTTAACATGTTAATTATTAC+AGG + chr5.4:13808857-13808876 MS.gene59476:intron 15.0%
!! GTTCATAACAAAAAAAAATC+TGG - chr5.4:13810100-13810119 None:intergenic 20.0%
!! TAATGAATCAAACTAATGAT+TGG + chr5.4:13809870-13809889 MS.gene59476:intron 20.0%
! AAAGAGTAAAAATTACCTTG+TGG - chr5.4:13809581-13809600 None:intergenic 25.0%
! GTGATAAGTTATTGAAGAAA+AGG - chr5.4:13809614-13809633 None:intergenic 25.0%
! GTTATTGAAGAAAAGGTAAA+CGG - chr5.4:13809607-13809626 None:intergenic 25.0%
! TAACAAAAAAAAATCTGGCA+TGG - chr5.4:13810095-13810114 None:intergenic 25.0%
! TAAGATAGAGAAACTTGAAT+GGG + chr5.4:13809978-13809997 MS.gene59476:intron 25.0%
! TATAGTTATGCTTATGATGA+TGG + chr5.4:13809496-13809515 MS.gene59476:CDS 25.0%
! TTAAGATAGAGAAACTTGAA+TGG + chr5.4:13809977-13809996 MS.gene59476:intron 25.0%
AAAAAAAAATCTGGCATGGA+CGG - chr5.4:13810091-13810110 None:intergenic 30.0%
AAAAAACTAAACTGCATGTC+TGG - chr5.4:13808784-13808803 None:intergenic 30.0%
AAAGTGATGAGTAGTAATAG+CGG + chr5.4:13809328-13809347 MS.gene59476:CDS 30.0%
AAGATAGAGAAACTTGAATG+GGG + chr5.4:13809979-13809998 MS.gene59476:intron 30.0%
AATGCAGTTCAATCAAATCT+GGG + chr5.4:13810280-13810299 MS.gene59476:intron 30.0%
ACTACGAAAATCGAATAACT+TGG - chr5.4:13808686-13808705 None:intergenic 30.0%
AGTAAAAATTACCTTGTGGA+GGG - chr5.4:13809577-13809596 None:intergenic 30.0%
CACAAAAACTAACTCATACA+CGG - chr5.4:13810157-13810176 None:intergenic 30.0%
TGAGATAGGAATATTGCTAT+TGG + chr5.4:13809924-13809943 MS.gene59476:intron 30.0%
TTCACAATTGTGAACAATTG+CGG + chr5.4:13808896-13808915 MS.gene59476:CDS 30.0%
! AATAAAGACAGCAGAGATTT+TGG + chr5.4:13809706-13809725 MS.gene59476:intron 30.0%
!!! AGTTTTATTGATGGTACATC+TGG - chr5.4:13810132-13810151 None:intergenic 30.0%
!!! GTTAAAACGTGTTTTGAGAT+AGG + chr5.4:13809910-13809929 MS.gene59476:intron 30.0%
!!! TACACGGAAAGTTTTATTGA+TGG - chr5.4:13810141-13810160 None:intergenic 30.0%
!!! TGTTATTGCCTTTGTTTTGA+TGG + chr5.4:13810383-13810402 MS.gene59476:CDS 30.0%
AAGAGTCAACAACGTTGTAA+CGG + chr5.4:13810359-13810378 MS.gene59476:CDS 35.0%
ACGAAAATCGAATAACTTGG+CGG - chr5.4:13808683-13808702 None:intergenic 35.0%
ACTCTTAAAAAACTGCGAGT+AGG - chr5.4:13809461-13809480 None:intergenic 35.0%
AGTTCAATCAAATCTGGGAA+TGG + chr5.4:13810285-13810304 MS.gene59476:intron 35.0%
ATTCTCTACCAACTCCTAAT+AGG + chr5.4:13808649-13808668 MS.gene59476:CDS 35.0%
GAATGCAGTTCAATCAAATC+TGG + chr5.4:13810279-13810298 MS.gene59476:intron 35.0%
GAATGGAAAATATCCTTTGC+CGG + chr5.4:13810302-13810321 MS.gene59476:CDS 35.0%
GAGTAAAAATTACCTTGTGG+AGG - chr5.4:13809578-13809597 None:intergenic 35.0%
GTTCATTCGCACTAAAATGA+AGG - chr5.4:13809773-13809792 None:intergenic 35.0%
!! TAGGAGTTGGTAGAGAATAA+GGG - chr5.4:13808647-13808666 None:intergenic 35.0%
!! TTAGGAGTTGGTAGAGAATA+AGG - chr5.4:13808648-13808667 None:intergenic 35.0%
!! TTAGTTTTTGTGTAGCTAGC+TGG + chr5.4:13810165-13810184 MS.gene59476:intron 35.0%
AAAAACTGCGAGTAGGAACT+TGG - chr5.4:13809454-13809473 None:intergenic 40.0%
ACAATTGCGGTTACACAGTT+TGG + chr5.4:13808909-13808928 MS.gene59476:CDS 40.0%
CAACGTGACAACCATATCTA+AGG - chr5.4:13810033-13810052 None:intergenic 40.0%
CACCAAAGCCATCAAAACAA+AGG - chr5.4:13810394-13810413 None:intergenic 40.0%
CGAAAATCGAATAACTTGGC+GGG - chr5.4:13808682-13808701 None:intergenic 40.0%
GAACTTGGTTTACACGTATC+AGG - chr5.4:13809439-13809458 None:intergenic 40.0%
GAGTGTTACCTATTAGGAGT+TGG - chr5.4:13808660-13808679 None:intergenic 40.0%
GGAAAATTCTCATGCATCAC+CGG + chr5.4:13809076-13809095 MS.gene59476:CDS 40.0%
GTGAATTCTGCTAATGTTGC+CGG - chr5.4:13809151-13809170 None:intergenic 40.0%
TAAGCATAACTATAAGCGCG+TGG - chr5.4:13809490-13809509 None:intergenic 40.0%
TACTCAATTTGCCACCACTT+TGG + chr5.4:13808618-13808637 MS.gene59476:CDS 40.0%
TGCATGATCTAGCGTGATTA+TGG + chr5.4:13808741-13808760 MS.gene59476:intron 40.0%
TTAGCAGAATTCACCCTCAA+CGG + chr5.4:13809157-13809176 MS.gene59476:CDS 40.0%
! ATGCATGAGAATTTTCCGGT+GGG - chr5.4:13809073-13809092 None:intergenic 40.0%
! GGTGATGCATGAGAATTTTC+CGG - chr5.4:13809077-13809096 None:intergenic 40.0%
! TATTGATGGTACATCTGGCA+TGG - chr5.4:13810127-13810146 None:intergenic 40.0%
!!! CTTTGTTTTGATGGCTTTGG+TGG + chr5.4:13810392-13810411 MS.gene59476:CDS 40.0%
!!! TGCCTTTGTTTTGATGGCTT+TGG + chr5.4:13810389-13810408 MS.gene59476:CDS 40.0%
AAACTTGAATGGGGACCTGT+TGG + chr5.4:13809988-13810007 MS.gene59476:intron 45.0%
AAATTACCTTGTGGAGGGTG+TGG - chr5.4:13809572-13809591 None:intergenic 45.0%
AATAAGGGCAACCAAAGTGG+TGG - chr5.4:13808632-13808651 None:intergenic 45.0%
AATTCTGCTAATGTTGCCGG+TGG - chr5.4:13809148-13809167 None:intergenic 45.0%
AGTGTGTTCTTCCCCATAGA+CGG - chr5.4:13809039-13809058 None:intergenic 45.0%
CCGATATTGATCGAACCACA+CGG + chr5.4:13809232-13809251 MS.gene59476:CDS 45.0%
CCGTGTGGTTCGATCAATAT+CGG - chr5.4:13809235-13809254 None:intergenic 45.0%
CGTGTGGTTCGATCAATATC+GGG - chr5.4:13809234-13809253 None:intergenic 45.0%
CTCATCACTTTCAACTCCGT+CGG - chr5.4:13809319-13809338 None:intergenic 45.0%
GAGAATAAGGGCAACCAAAG+TGG - chr5.4:13808635-13808654 None:intergenic 45.0%
GTACGGCATCACCTTAGATA+TGG + chr5.4:13810019-13810038 MS.gene59476:intron 45.0%
GTCGAAGAAATCAAGTCCTC+CGG - chr5.4:13809185-13809204 None:intergenic 45.0%
GTGATGAGTAGTAATAGCGG+CGG + chr5.4:13809331-13809350 MS.gene59476:CDS 45.0%
TTGCTCCGTCAACTTAAACG+CGG + chr5.4:13809288-13809307 MS.gene59476:CDS 45.0%
! AAAAGTGCGTGTGAAGCGTT+TGG + chr5.4:13809376-13809395 MS.gene59476:CDS 45.0%
! AAAGTGCGTGTGAAGCGTTT+GGG + chr5.4:13809377-13809396 MS.gene59476:CDS 45.0%
! AATTACCTTGTGGAGGGTGT+GGG - chr5.4:13809571-13809590 None:intergenic 45.0%
! ACCTGTTGGCTGTTATGGTA+CGG + chr5.4:13810002-13810021 MS.gene59476:intron 45.0%
! GATGCATGAGAATTTTCCGG+TGG - chr5.4:13809074-13809093 None:intergenic 45.0%
! GTTTGGCCGGGAATTTTATC+CGG + chr5.4:13808926-13808945 MS.gene59476:CDS 45.0%
! TGCATGAGAATTTTCCGGTG+GGG - chr5.4:13809072-13809091 None:intergenic 45.0%
!! TGATGGCTTTGGTGGTTGAA+CGG + chr5.4:13810400-13810419 MS.gene59476:CDS 45.0%
!! CTTAATAATTAAATTAAAAA+AGG - chr5.4:13809962-13809981 None:intergenic 5.0%
ACAGAACCATTCTCCACCAC+CGG + chr5.4:13808956-13808975 MS.gene59476:CDS 50.0%
ATCGAACCACACGGTGAAAC+CGG + chr5.4:13809241-13809260 MS.gene59476:CDS 50.0%
ATGAGTAGTAATAGCGGCGG+AGG + chr5.4:13809334-13809353 MS.gene59476:CDS 50.0%
CGGAAGAAACGCTATACCAC+CGG + chr5.4:13809129-13809148 MS.gene59476:CDS 50.0%
CGGTGGTATAGCGTTTCTTC+CGG - chr5.4:13809131-13809150 None:intergenic 50.0%
GAATTCACCCTCAACGGTTC+CGG + chr5.4:13809163-13809182 MS.gene59476:CDS 50.0%
GATCCGCAATATTGCTGCAG+TGG + chr5.4:13809400-13809419 MS.gene59476:CDS 50.0%
GTTTCTTCCGGCACATTCGA+TGG - chr5.4:13809119-13809138 None:intergenic 50.0%
TATTGCTGCAGTGGCGCTTA+TGG + chr5.4:13809409-13809428 MS.gene59476:CDS 50.0%
TTGCGGTTACACAGTTTGGC+CGG + chr5.4:13808913-13808932 MS.gene59476:CDS 50.0%
! AAGTGCGTGTGAAGCGTTTG+GGG + chr5.4:13809378-13809397 MS.gene59476:CDS 50.0%
! ATTACCTTGTGGAGGGTGTG+GGG - chr5.4:13809570-13809589 None:intergenic 50.0%
! CTTTGGTGGTTGAACGGTTG+TGG + chr5.4:13810406-13810425 MS.gene59476:CDS 50.0%
! GGTGGAGAATGGTTCTGTTC+CGG - chr5.4:13808954-13808973 None:intergenic 50.0%
! TTCGACGTTAGTCTCGTCGA+CGG + chr5.4:13809199-13809218 MS.gene59476:CDS 50.0%
!! ACACGCCGCGTTTAAGTTGA+CGG - chr5.4:13809296-13809315 None:intergenic 50.0%
!! TGGCGGGAGTGTTACCTATT+AGG - chr5.4:13808666-13808685 None:intergenic 50.0%
AAGTCCTCCGGAACCGTTGA+GGG - chr5.4:13809173-13809192 None:intergenic 55.0%
ACACCAGACACGTCTGTAGC+CGG - chr5.4:13810324-13810343 None:intergenic 55.0%
CGTGTCTGGTGTTCGACATG+TGG + chr5.4:13810332-13810351 MS.gene59476:CDS 55.0%
GCCGTACCATAACAGCCAAC+AGG - chr5.4:13810006-13810025 None:intergenic 55.0%
GCGCCACTGCAGCAATATTG+CGG - chr5.4:13809406-13809425 None:intergenic 55.0%
GGGTGTGGGGCAAAAAGTGA+TGG - chr5.4:13809557-13809576 None:intergenic 55.0%
TCCTCCACCATCGAATGTGC+CGG + chr5.4:13809109-13809128 MS.gene59476:CDS 55.0%
TCTTCCGGCACATTCGATGG+TGG - chr5.4:13809116-13809135 None:intergenic 55.0%
TGCGGTTACACAGTTTGGCC+GGG + chr5.4:13808914-13808933 MS.gene59476:CDS 55.0%
TTCCTGGTCTGGCCGTCTAT+GGG + chr5.4:13809024-13809043 MS.gene59476:CDS 55.0%
TTGGAGAACAAAGCCGGTGG+TGG - chr5.4:13808972-13808991 None:intergenic 55.0%
! TGGGGACCTGTTGGCTGTTA+TGG + chr5.4:13809997-13810016 MS.gene59476:intron 55.0%
!! ACAAAGCCGGTGGTGGAGAA+TGG - chr5.4:13808965-13808984 None:intergenic 55.0%
!! ACCGGCTTTGTTCTCCAACC+CGG + chr5.4:13808974-13808993 MS.gene59476:CDS 55.0%
CAAGTCCTCCGGAACCGTTG+AGG - chr5.4:13809174-13809193 None:intergenic 60.0%
CACTGCTCTCAAGACCCCAC+CGG + chr5.4:13809055-13809074 MS.gene59476:CDS 60.0%
CATAGACGGCCAGACCAGGA+AGG - chr5.4:13809025-13809044 None:intergenic 60.0%
CTTAAACGCGGCGTGTCCGA+CGG + chr5.4:13809300-13809319 MS.gene59476:CDS 60.0%
CTTCCTGGTCTGGCCGTCTA+TGG + chr5.4:13809023-13809042 MS.gene59476:CDS 60.0%
GAACCACACGGTGAAACCGG+CGG + chr5.4:13809244-13809263 MS.gene59476:CDS 60.0%
GACACGTCTGTAGCCGGCAA+AGG - chr5.4:13810318-13810337 None:intergenic 60.0%
GCCAGACCAGGAAGGTGGTA+CGG - chr5.4:13809017-13809036 None:intergenic 60.0%
GGGTTGGAGAACAAAGCCGG+TGG - chr5.4:13808975-13808994 None:intergenic 60.0%
GGTGGAGGAATCACAGTCGC+CGG - chr5.4:13809098-13809117 None:intergenic 60.0%
TCCTGGTCTGGCCGTCTATG+GGG + chr5.4:13809025-13809044 MS.gene59476:CDS 60.0%
TTCACCCTCAACGGTTCCGG+AGG + chr5.4:13809166-13809185 MS.gene59476:CDS 60.0%
TTTGCCCCACACCCTCCACA+AGG + chr5.4:13809563-13809582 MS.gene59476:CDS 60.0%
! CCGGCGCCGGATAAAATTCC+CGG - chr5.4:13808935-13808954 None:intergenic 60.0%
! CCGGGAATTTTATCCGGCGC+CGG + chr5.4:13808932-13808951 MS.gene59476:CDS 60.0%
! GAATGGTTCTGTTCCGGCGC+CGG - chr5.4:13808948-13808967 None:intergenic 60.0%
! GCCGGGTTGGAGAACAAAGC+CGG - chr5.4:13808978-13808997 None:intergenic 60.0%
! TCCGGCACATTCGATGGTGG+AGG - chr5.4:13809113-13809132 None:intergenic 60.0%
! TTGCCGGCTACAGACGTGTC+TGG + chr5.4:13810318-13810337 MS.gene59476:CDS 60.0%
AACCGGCGGCGGAAACTGCA+CGG + chr5.4:13809258-13809277 MS.gene59476:CDS 65.0%
AGACGGCCAGACCAGGAAGG+TGG - chr5.4:13809022-13809041 None:intergenic 65.0%
CGGCGGAAACTGCACGGCAA+CGG + chr5.4:13809264-13809283 MS.gene59476:CDS 65.0%
CTCTCGCCGTACCACCTTCC+TGG + chr5.4:13809008-13809027 MS.gene59476:CDS 65.0%
GCCGTACCACCTTCCTGGTC+TGG + chr5.4:13809013-13809032 MS.gene59476:CDS 65.0%
GGCGGAAACTGCACGGCAAC+GGG + chr5.4:13809265-13809284 MS.gene59476:CDS 65.0%
TCCCCATAGACGGCCAGACC+AGG - chr5.4:13809029-13809048 None:intergenic 65.0%
! AGGTGGTACGGCGAGAGCGT+TGG - chr5.4:13809005-13809024 None:intergenic 65.0%
CCACACGGTGAAACCGGCGG+CGG + chr5.4:13809247-13809266 MS.gene59476:CDS 70.0%
CGGCGGAGGTGAAGAGAGCG+TGG + chr5.4:13809348-13809367 MS.gene59476:CDS 70.0%
TGCCGTGCAGTTTCCGCCGC+CGG - chr5.4:13809263-13809282 None:intergenic 70.0%
!! GAGCGTTGGACTCGCCGGGT+TGG - chr5.4:13808991-13809010 None:intergenic 70.0%
!! GCGAGAGCGTTGGACTCGCC+GGG - chr5.4:13808995-13809014 None:intergenic 70.0%
!! GGCGAGAGCGTTGGACTCGC+CGG - chr5.4:13808996-13809015 None:intergenic 70.0%
!! CCGCCGCCGGTTTCACCGTG+TGG - chr5.4:13809250-13809269 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 13808607 13810431 13808607 ID=MS.gene59476
chr5.4 mRNA 13808607 13810431 13808607 ID=MS.gene59476.t1;Parent=MS.gene59476
chr5.4 exon 13808607 13808670 13808607 ID=MS.gene59476.t1.exon1;Parent=MS.gene59476.t1
chr5.4 CDS 13808607 13808670 13808607 ID=cds.MS.gene59476.t1;Parent=MS.gene59476.t1
chr5.4 exon 13808879 13809584 13808879 ID=MS.gene59476.t1.exon2;Parent=MS.gene59476.t1
chr5.4 CDS 13808879 13809584 13808879 ID=cds.MS.gene59476.t1;Parent=MS.gene59476.t1
chr5.4 exon 13810287 13810431 13810287 ID=MS.gene59476.t1.exon3;Parent=MS.gene59476.t1
chr5.4 CDS 13810287 13810431 13810287 ID=cds.MS.gene59476.t1;Parent=MS.gene59476.t1
Gene Sequence

>MS.gene59476

ATGGCTTCTTCTACTCAATTTGCCACCACTTTGGTTGCCCTTATTCTCTACCAACTCCTAATAGGTAACACTCCCGCCAAGTTATTCGATTTTCGTAGTGCATGTTTGAATTCAAGATGAATTTGTTAGAATCATGCATGATCTAGCGTGATTATGGCAAATCTAACAATGATGCCAGACATGCAGTTTAGTTTTTTTTGTTGTTTTTAATGTTCACATAAAAGTTTGTGTCTTAAGTTGCTTTCTTGTTTTTAACATGTTAATTATTACAGGTTCATATTCATCAACATTCACAATTGTGAACAATTGCGGTTACACAGTTTGGCCGGGAATTTTATCCGGCGCCGGAACAGAACCATTCTCCACCACCGGCTTTGTTCTCCAACCCGGCGAGTCCAACGCTCTCGCCGTACCACCTTCCTGGTCTGGCCGTCTATGGGGAAGAACACACTGCTCTCAAGACCCCACCGGAAAATTCTCATGCATCACCGGCGACTGTGATTCCTCCACCATCGAATGTGCCGGAAGAAACGCTATACCACCGGCAACATTAGCAGAATTCACCCTCAACGGTTCCGGAGGACTTGATTTCTTCGACGTTAGTCTCGTCGACGGTTACAATCTCCCGATATTGATCGAACCACACGGTGAAACCGGCGGCGGAAACTGCACGGCAACGGGTTGCTCCGTCAACTTAAACGCGGCGTGTCCGACGGAGTTGAAAGTGATGAGTAGTAATAGCGGCGGAGGTGAAGAGAGCGTGGCGTGTAAAAGTGCGTGTGAAGCGTTTGGGGATCCGCAATATTGCTGCAGTGGCGCTTATGGTTCGCCTGATACGTGTAAACCAAGTTCCTACTCGCAGTTTTTTAAGAGTGCGTGTCCACGCGCTTATAGTTATGCTTATGATGATGGAACTAGCACTTTCACTTGTGCTTCTGCTGATTATACCATCACTTTTTGCCCCACACCCTCCACAAGGTAATTTTTACTCTTTTTACCGTTTACCTTTTCTTCAATAACTTATCACAATTTTCTTTTATTTTCAAAAAGTATAATTGGTTGTTAAAATATCTAGTGAATTGTCAAATATCACTCAAATAATAAAGACAGCAGAGATTTTGGATAAAAGAATGTAAAAATATTTGATATGTACGAGATTCGAACCTTCATTTTAGTGCGAATGAACTTTATCACTAAATTTTTTCATAACAAAAAAATGAGTATTAGAGAATGTTCAAAAATGAGTGTTGTTAGCGTTCTGATTAATGAATCAAACTAATGATTGGAATTGTTATTCTTAGTTGTTAAAACGTGTTTTGAGATAGGAATATTGCTATTGGATTTTCATATGACCTTTTTTAATTTAATTATTAAGATAGAGAAACTTGAATGGGGACCTGTTGGCTGTTATGGTACGGCATCACCTTAGATATGGTTGTCACGTTGCATTTTTTTCTGTTTTATTTATATATACATATGCACCGTCCATGCCAGATTTTTTTTTGTTATGAACTATGCCATGCCAGATGTACCATCAATAAAACTTTCCGTGTATGAGTTAGTTTTTGTGTAGCTAGCTGGTTTTGTTTTGTGTTGAAGTTTTTTTTTTTTTTTTTTGAATAATTGTGTTGAAGTTTGAGTGCAACATTTTGAAAAAATTGTGTTTGAATGTGAATGCAGTTCAATCAAATCTGGGAATGGAAAATATCCTTTGCCGGCTACAGACGTGTCTGGTGTTCGACATGTGGATAAAAGAGTCAACAACGTTGTAACGGTTGTTATTGCCTTTGTTTTGATGGCTTTGGTGGTTGAACGGTTGTGGTAA
Protein sequence

>MS.gene59476.t1

MASSTQFATTLVALILYQLLIGSYSSTFTIVNNCGYTVWPGILSGAGTEPFSTTGFVLQPGESNALAVPPSWSGRLWGRTHCSQDPTGKFSCITGDCDSSTIECAGRNAIPPATLAEFTLNGSGGLDFFDVSLVDGYNLPILIEPHGETGGGNCTATGCSVNLNAACPTELKVMSSNSGGGEESVACKSACEAFGDPQYCCSGAYGSPDTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCASADYTITFCPTPSTSSIKSGNGKYPLPATDVSGVRHVDKRVNNVVTVVIAFVLMALVVERLW