AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92393

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92393.t1 MTR_8g037890 86.120 317 20 1 1 293 1 317 0.0 540
MS.gene92393.t1 MTR_8g056820 88.000 250 30 0 1 250 1 250 4.50e-161 449
MS.gene92393.t1 MTR_5g022310 78.082 292 55 5 1 284 1 291 1.28e-159 447
MS.gene92393.t1 MTR_5g022310 82.812 256 40 2 1 252 1 256 1.12e-151 425
MS.gene92393.t1 MTR_8g075550 64.078 309 93 6 1 295 1 305 1.71e-130 374
MS.gene92393.t1 MTR_1g025420 61.765 272 97 5 14 280 11 280 6.26e-114 332
MS.gene92393.t1 MTR_1g025420 67.078 243 74 4 14 251 11 252 6.00e-112 325
MS.gene92393.t1 MTR_3g114030 67.949 234 71 3 20 250 1 233 5.74e-110 319
MS.gene92393.t1 MTR_4g073730 62.857 245 82 3 11 247 5 248 7.15e-108 316
MS.gene92393.t1 MTR_4g073730 62.857 245 82 3 11 247 5 248 1.25e-107 316
MS.gene92393.t1 MTR_4g073720 59.167 240 87 5 9 246 9 239 4.05e-91 275
MS.gene92393.t1 MTR_5g022350 52.158 278 118 7 1 272 4 272 6.62e-87 263
MS.gene92393.t1 MTR_8g075510 54.582 251 102 7 3 252 10 249 6.82e-83 251
MS.gene92393.t1 MTR_8g075510 54.694 245 99 7 3 246 10 243 1.21e-80 247
MS.gene92393.t1 MTR_2g069660 51.406 249 113 4 1 247 1 243 2.89e-80 244
MS.gene92393.t1 MTR_2g068030 52.489 221 101 3 28 246 25 243 5.36e-80 243
MS.gene92393.t1 MTR_2g067980 54.113 231 100 3 24 250 22 250 1.78e-79 261
MS.gene92393.t1 MTR_7g102380 49.794 243 116 5 9 249 5 243 5.16e-79 241
MS.gene92393.t1 MTR_8g036215 49.791 239 115 4 9 246 3 237 1.89e-77 236
MS.gene92393.t1 MTR_1g021945 53.947 228 99 4 25 250 25 248 4.39e-76 233
MS.gene92393.t1 MTR_2g068655 48.927 233 117 2 22 253 27 258 1.67e-75 234
MS.gene92393.t1 MTR_3g111620 53.070 228 101 5 25 250 26 249 4.54e-75 231
MS.gene92393.t1 MTR_6g079580 50.622 241 110 5 11 247 5 240 9.44e-75 229
MS.gene92393.t1 MTR_1g062590 48.178 247 120 5 3 247 2 242 4.09e-74 228
MS.gene92393.t1 MTR_1g062660 50.628 239 111 5 13 249 11 244 4.31e-74 228
MS.gene92393.t1 MTR_1g062340 50.644 233 108 5 19 249 17 244 1.18e-73 227
MS.gene92393.t1 MTR_8g107140 49.200 250 119 5 1 247 1 245 2.69e-73 226
MS.gene92393.t1 MTR_1g062350 49.194 248 118 6 4 249 3 244 2.58e-72 223
MS.gene92393.t1 MTR_1g062370 48.387 248 120 6 4 249 3 244 5.06e-72 223
MS.gene92393.t1 MTR_5g023850 47.244 254 95 4 1 250 1 219 3.61e-71 220
MS.gene92393.t1 MTR_1g062630 48.207 251 122 6 3 251 2 246 1.68e-69 217
MS.gene92393.t1 MTR_5g059200 45.703 256 124 7 1 252 1 245 4.13e-68 214
MS.gene92393.t1 MTR_7g062610 45.455 242 122 4 7 247 10 242 3.51e-67 210
MS.gene92393.t1 MTR_3g068015 47.556 225 113 4 25 247 27 248 2.82e-66 208
MS.gene92393.t1 MTR_1g062690 44.355 248 106 7 4 249 3 220 8.10e-59 188
MS.gene92393.t1 MTR_2g067990 46.552 232 96 8 19 249 20 224 1.31e-58 202
MS.gene92393.t1 MTR_6g009480 40.000 250 146 4 1 247 4 252 5.01e-58 188
MS.gene92393.t1 MTR_4g063630 42.739 241 126 8 13 246 16 251 1.98e-57 186
MS.gene92393.t1 MTR_2g067970 47.568 185 92 5 69 250 3 185 1.17e-47 171
MS.gene92393.t1 MTR_5g010640 39.608 255 127 9 1 247 1 236 1.24e-46 157
MS.gene92393.t1 MTR_5g010635 39.823 226 114 7 23 247 21 225 3.92e-44 151
MS.gene92393.t1 MTR_8g096900 39.331 239 119 9 11 247 34 248 1.61e-41 144
MS.gene92393.t1 MTR_5g022350 48.026 152 73 3 1 150 69 216 5.96e-39 137
MS.gene92393.t1 MTR_2g063150 34.906 212 129 5 27 236 35 239 1.00e-37 135
MS.gene92393.t1 MTR_8g089020 50.000 130 60 2 110 239 32 156 3.54e-36 128
MS.gene92393.t1 MTR_1g062390 31.225 253 153 8 1 247 1 238 4.66e-31 117
MS.gene92393.t1 MTR_7g076360 35.377 212 73 4 25 236 7 154 1.93e-30 113
MS.gene92393.t1 MTR_8g096910 43.885 139 62 6 9 141 1 129 9.31e-28 106
MS.gene92393.t1 MTR_8g096920 40.580 138 71 5 9 144 1 129 5.29e-23 94.0
MS.gene92393.t1 MTR_3g081550 48.421 95 46 2 111 203 16 109 8.28e-19 80.9
MS.gene92393.t1 MTR_1g062640 38.889 90 52 2 79 168 60 146 9.11e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92393.t1 AT4G36010 69.957 233 64 3 22 248 19 251 2.01e-116 338
MS.gene92393.t1 AT4G36010 69.957 233 64 3 22 248 19 251 2.01e-116 338
MS.gene92393.t1 AT2G17860 65.289 242 79 3 14 250 11 252 6.46e-110 320
MS.gene92393.t1 AT1G75800 59.459 259 96 5 9 259 5 262 3.83e-105 310
MS.gene92393.t1 AT1G20030 52.980 302 113 6 22 295 16 316 2.62e-103 305
MS.gene92393.t1 AT1G20030 53.177 299 111 6 25 295 2 299 2.93e-103 304
MS.gene92393.t1 AT4G38670 60.156 256 94 5 9 257 6 260 4.68e-103 303
MS.gene92393.t1 AT4G38670 60.156 256 94 5 9 257 6 260 4.68e-103 303
MS.gene92393.t1 AT4G38670 63.866 238 78 5 27 257 8 244 1.78e-102 301
MS.gene92393.t1 AT4G38660 57.588 257 101 4 3 259 9 257 2.76e-95 286
MS.gene92393.t1 AT4G24180 55.859 256 101 5 1 248 6 257 8.00e-95 281
MS.gene92393.t1 AT4G24180 55.859 256 101 5 1 248 6 257 1.89e-94 282
MS.gene92393.t1 AT4G24180 58.921 241 89 4 14 248 16 252 1.97e-94 280
MS.gene92393.t1 AT4G24180 58.921 241 89 4 14 248 16 252 5.32e-94 281
MS.gene92393.t1 AT4G38660 59.583 240 91 3 20 259 2 235 1.85e-93 280
MS.gene92393.t1 AT4G24180 53.750 240 99 5 1 232 6 241 5.43e-84 256
MS.gene92393.t1 AT4G24180 56.889 225 87 4 14 232 16 236 1.77e-83 255
MS.gene92393.t1 AT5G38280 43.986 291 150 5 7 291 5 288 4.73e-82 261
MS.gene92393.t1 AT5G38280 43.986 291 150 5 7 291 5 288 1.01e-81 260
MS.gene92393.t1 AT1G75030 53.629 248 104 8 9 250 4 246 1.47e-81 247
MS.gene92393.t1 AT5G24620 50.758 264 116 5 9 265 7 263 3.38e-81 252
MS.gene92393.t1 AT5G24620 53.012 249 104 4 23 265 77 318 4.89e-81 253
MS.gene92393.t1 AT5G24620 53.012 249 104 4 23 265 98 339 5.46e-81 254
MS.gene92393.t1 AT1G75050 55.217 230 96 5 23 250 22 246 3.24e-79 241
MS.gene92393.t1 AT1G75040 53.333 240 106 4 8 247 6 239 1.67e-78 239
MS.gene92393.t1 AT1G19320 50.199 251 115 5 1 246 1 246 2.32e-78 239
MS.gene92393.t1 AT1G18250 51.982 227 102 4 23 247 19 240 2.09e-76 234
MS.gene92393.t1 AT1G18250 52.000 225 101 4 25 247 20 239 3.09e-76 233
MS.gene92393.t1 AT1G77700 41.254 303 155 8 12 310 43 326 7.96e-76 235
MS.gene92393.t1 AT1G73620 52.444 225 100 4 25 247 41 260 8.46e-76 233
MS.gene92393.t1 AT1G77700 42.657 286 145 7 25 310 88 354 5.31e-75 234
MS.gene92393.t1 AT1G77700 48.954 239 108 6 12 246 43 271 2.93e-73 228
MS.gene92393.t1 AT4G36000 66.038 159 45 3 102 259 1 151 1.55e-66 207
MS.gene92393.t1 AT4G36000 66.038 159 45 3 102 259 1 151 1.55e-66 207
MS.gene92393.t1 AT5G02140 41.219 279 154 5 8 285 6 275 7.05e-66 209
MS.gene92393.t1 AT5G02140 43.983 241 129 3 8 247 6 241 4.03e-64 202
MS.gene92393.t1 AT4G18253 42.857 245 129 4 9 250 5 241 5.18e-61 194
MS.gene92393.t1 AT2G28790 44.628 242 125 7 10 246 11 248 2.59e-60 193
MS.gene92393.t1 AT2G28790 44.628 242 125 7 10 246 36 273 2.80e-60 194
MS.gene92393.t1 AT4G18250 43.320 247 131 5 5 249 35 274 1.72e-57 196
MS.gene92393.t1 AT5G40020 40.611 229 131 4 23 249 25 250 1.45e-55 181
MS.gene92393.t1 AT1G70250 42.149 242 121 8 25 255 144 377 2.47e-55 192
MS.gene92393.t1 AT4G11650 40.392 255 127 9 11 261 5 238 4.76e-52 172
MS.gene92393.t1 AT5G24620 61.667 120 41 1 133 247 15 134 1.78e-42 149
MS.gene92393.t1 AT2G24810 59.740 77 31 0 170 246 116 192 7.27e-27 104

Find 91 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGGTATTGGAATAGAATT+AGG 0.184672 8.4:-60373200 None:intergenic
CCAAGTGGTCAGGCCGTCTA+TGG 0.207746 8.4:+60373370 MS.gene92393:CDS
TCTGAGGAGATGGATATATC+TGG 0.261477 8.4:+60373942 MS.gene92393:CDS
TGTTGTTCTTGTGGTGAATT+TGG 0.294332 8.4:+60374070 MS.gene92393:CDS
GAACTTGGCTTGCATGTATC+TGG 0.295884 8.4:-60373768 None:intergenic
GGAAAATTCTCCTGTGTTAC+CGG 0.333338 8.4:+60373423 MS.gene92393:CDS
AAAGCGCATGTGAAGCGTTT+GGG 0.344124 8.4:+60373709 MS.gene92393:CDS
CCATGTGGTTCGATCGTCAT+TGG 0.344279 8.4:-60373579 None:intergenic
CCCACAAGTTCGATCCAATC+TGG 0.346023 8.4:+60373900 MS.gene92393:CDS
ATTCTATTCCAATACCTAAC+TGG 0.349170 8.4:+60373204 MS.gene92393:CDS
GGCAAGGGCATTGGATTCGC+TGG 0.362141 8.4:-60373340 None:intergenic
TGTAGAGAACGGTGTTGTTC+CGG 0.383816 8.4:-60373298 None:intergenic
CCGGCGCCGGATAAAATTCC+AGG 0.384166 8.4:-60373279 None:intergenic
CATATGAACCAGTTAGGTAT+TGG 0.386121 8.4:-60373212 None:intergenic
GAGATTTCTCGTTCCCAGAT+TGG 0.394550 8.4:-60373914 None:intergenic
AACAACAATCATAACATTGT+TGG 0.405286 8.4:-60373985 None:intergenic
GTTTCCTCCAGTACATTCTA+TGG 0.405913 8.4:-60373463 None:intergenic
CAAGTGGTCAGGCCGTCTAT+GGG 0.409833 8.4:+60373371 MS.gene92393:CDS
AACTCAATATACCATCGCTT+TGG 0.419138 8.4:+60373173 MS.gene92393:CDS
TTAGCCGAGTTTACACTTAA+CGG 0.420790 8.4:+60373504 MS.gene92393:CDS
CTCATCACTTTCAACTCGGT+TGG 0.422630 8.4:-60373663 None:intergenic
CCATAGACGGCCTGACCACT+TGG 0.423008 8.4:-60373370 None:intergenic
ACGAGTGCAACTACACAGTT+TGG 0.425166 8.4:+60373256 MS.gene92393:CDS
GGGAGGTACGGCAAGGGCAT+TGG 0.430183 8.4:-60373349 None:intergenic
GCCGTACCTCCCAAGTGGTC+AGG 0.435086 8.4:+60373360 MS.gene92393:CDS
TGTGGTGAATTTGGTAGGCT+AGG 0.436989 8.4:+60374079 MS.gene92393:CDS
ATCGAACCACATGGAGCAAC+CGG 0.442103 8.4:+60373588 MS.gene92393:CDS
TGTCATGCAGTTTCCGCCTC+CGG 0.443562 8.4:-60373607 None:intergenic
TTGTTGCATATGAACCAGTT+AGG 0.449950 8.4:-60373218 None:intergenic
ACAACACCGTTCTCTACAAC+CGG 0.459009 8.4:+60373303 MS.gene92393:CDS
TAGGTATTGGAATAGAATTA+GGG 0.461104 8.4:-60373199 None:intergenic
CCACATGGAGCAACCGGAGG+CGG 0.465833 8.4:+60373594 MS.gene92393:CDS
GGCGGAAACTGCATGACAAC+AGG 0.472230 8.4:+60373612 MS.gene92393:CDS
TATAGCTACGCTTATGATGA+TGG 0.472777 8.4:+60373828 MS.gene92393:CDS
GCCTGACCACTTGGGAGGTA+CGG 0.475859 8.4:-60373361 None:intergenic
AAAAGCGCATGTGAAGCGTT+TGG 0.476727 8.4:+60373708 MS.gene92393:CDS
CATGTGGTTCGATCGTCATT+GGG 0.481504 8.4:-60373578 None:intergenic
ATATCCATCTCCTCAGACAT+TGG 0.484864 8.4:-60373936 None:intergenic
ATCTCCAATGTCTGAGGAGA+TGG 0.486815 8.4:+60373932 MS.gene92393:CDS
CCAGATTGGATCGAACTTGT+GGG 0.490228 8.4:-60373900 None:intergenic
GCTGGGTTGGAGAGCAAAGC+CGG 0.490388 8.4:-60373322 None:intergenic
CTGAGGAGATGGATATATCT+GGG 0.492913 8.4:+60373943 MS.gene92393:CDS
TTTACACTTAACGGTGCAAA+CGG 0.497247 8.4:+60373513 MS.gene92393:CDS
GTTCTTGTGGTGAATTTGGT+AGG 0.497434 8.4:+60374074 MS.gene92393:CDS
CCCAGATTGGATCGAACTTG+TGG 0.498644 8.4:-60373901 None:intergenic
GGGCATTGGATTCGCTGGGT+TGG 0.505014 8.4:-60373335 None:intergenic
GAACGGTGTTGTTCCGGCGC+CGG 0.511342 8.4:-60373292 None:intergenic
TCTTCCACCATAGAATGTAC+TGG 0.519755 8.4:+60373456 MS.gene92393:CDS
TGCAACTACACAGTTTGGCC+TGG 0.523345 8.4:+60373261 MS.gene92393:CDS
CCACAAGTTCGATCCAATCT+GGG 0.541949 8.4:+60373901 MS.gene92393:CDS
GCAAGGGCATTGGATTCGCT+GGG 0.544468 8.4:-60373339 None:intergenic
TTTCTTCGACGTTAGTCTCG+TGG 0.547119 8.4:+60373542 MS.gene92393:CDS
AGAGGGTACGGCCCCATAGA+CGG 0.558662 8.4:-60373383 None:intergenic
CCGCCTCCGGTTGCTCCATG+TGG 0.564023 8.4:-60373594 None:intergenic
ACCACTTGGGAGGTACGGCA+AGG 0.566696 8.4:-60373356 None:intergenic
CTCTGCTCTCAAGATTCCAC+CGG 0.569324 8.4:+60373402 MS.gene92393:CDS
AAGCGCATGTGAAGCGTTTG+GGG 0.569335 8.4:+60373710 MS.gene92393:CDS
GTAAACTCGGCTAATGTAGC+CGG 0.573023 8.4:-60373495 None:intergenic
AATCTTGAGAGCAGAGGGTA+CGG 0.574479 8.4:-60373395 None:intergenic
CTGCATGACAACAGGTTGCA+TGG 0.576620 8.4:+60373620 MS.gene92393:CDS
GATCCACAATACTGTTGTAG+TGG 0.579781 8.4:+60373732 MS.gene92393:CDS
GGTGGAAGAAGCACAATCAC+CGG 0.580280 8.4:-60373442 None:intergenic
AGCACAATCACCGGTAACAC+AGG 0.584348 8.4:-60373433 None:intergenic
TGCACCGTTAAGTGTAAACT+CGG 0.588205 8.4:-60373508 None:intergenic
CATAGACGGCCTGACCACTT+GGG 0.589711 8.4:-60373369 None:intergenic
ATTACTCATCACTTTCAACT+CGG 0.591426 8.4:-60373667 None:intergenic
CGGTGGAATCTTGAGAGCAG+AGG 0.605968 8.4:-60373401 None:intergenic
CGAGAAATCTCCAATGTCTG+AGG 0.606622 8.4:+60373926 MS.gene92393:CDS
GCAAAGCCGGTTGTAGAGAA+CGG 0.610915 8.4:-60373309 None:intergenic
AATTAGGGTAACCAAAGCGA+TGG 0.614240 8.4:-60373184 None:intergenic
GAGTTGAAAGTGATGAGTAA+TGG 0.615914 8.4:+60373669 MS.gene92393:CDS
GTGGTTCGATCGTCATTGGG+AGG 0.619192 8.4:-60373575 None:intergenic
TTCGACGTTAGTCTCGTGGA+CGG 0.621410 8.4:+60373546 MS.gene92393:CDS
GGTGGAATCTTGAGAGCAGA+GGG 0.624367 8.4:-60373400 None:intergenic
TGGAGGAAACGCTCAACCAC+CGG 0.626355 8.4:+60373476 MS.gene92393:CDS
GCGCCACTACAACAGTATTG+TGG 0.632976 8.4:-60373735 None:intergenic
TCCTCCAGTACATTCTATGG+TGG 0.635302 8.4:-60373460 None:intergenic
AAGTGGTCAGGCCGTCTATG+GGG 0.639176 8.4:+60373372 MS.gene92393:CDS
AACTCGGCTAATGTAGCCGG+TGG 0.641121 8.4:-60373492 None:intergenic
AGACGGCCTGACCACTTGGG+AGG 0.643248 8.4:-60373366 None:intergenic
TGAGGAGATGGATATATCTG+GGG 0.644366 8.4:+60373944 MS.gene92393:CDS
CCACTTGGGAGGTACGGCAA+GGG 0.646374 8.4:-60373355 None:intergenic
CCCTTGCCGTACCTCCCAAG+TGG 0.650312 8.4:+60373355 MS.gene92393:CDS
GAACCACATGGAGCAACCGG+AGG 0.670618 8.4:+60373591 MS.gene92393:CDS
TTGAAAGTGATGAGTAATGG+CGG 0.675133 8.4:+60373672 MS.gene92393:CDS
GAGGAGATGGATATATCTGG+GGG 0.677404 8.4:+60373945 MS.gene92393:CDS
CCAATGACGATCGAACCACA+TGG 0.678704 8.4:+60373579 MS.gene92393:CDS
TCCACCATAGAATGTACTGG+AGG 0.679150 8.4:+60373459 MS.gene92393:CDS
AAAGTGATGAGTAATGGCGG+AGG 0.693219 8.4:+60373675 MS.gene92393:CDS
CATGACAACAGGTTGCATGG+TGG 0.757398 8.4:+60373623 MS.gene92393:CDS
TAAGCGTAGCTATAAGCACG+AGG 0.764294 8.4:-60373819 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTTTTTTTTTTTGA+AGG + chr8.4:60374028-60374047 MS.gene92393:CDS 10.0%
! AACAACAATCATAACATTGT+TGG - chr8.4:60373988-60374007 None:intergenic 25.0%
!!! TAGGTATTGGAATAGAATTA+GGG - chr8.4:60373202-60373221 None:intergenic 25.0%
!!! TTAGGTATTGGAATAGAATT+AGG - chr8.4:60373203-60373222 None:intergenic 25.0%
ATTACTCATCACTTTCAACT+CGG - chr8.4:60373670-60373689 None:intergenic 30.0%
ATTCTATTCCAATACCTAAC+TGG + chr8.4:60373204-60373223 MS.gene92393:CDS 30.0%
!!! TTGTTTTGATGTTGTTCTTG+TGG + chr8.4:60374061-60374080 MS.gene92393:CDS 30.0%
AACTCAATATACCATCGCTT+TGG + chr8.4:60373173-60373192 MS.gene92393:CDS 35.0%
CATATGAACCAGTTAGGTAT+TGG - chr8.4:60373215-60373234 None:intergenic 35.0%
GAGTTGAAAGTGATGAGTAA+TGG + chr8.4:60373669-60373688 MS.gene92393:CDS 35.0%
TATAGCTACGCTTATGATGA+TGG + chr8.4:60373828-60373847 MS.gene92393:CDS 35.0%
TGTTGTTCTTGTGGTGAATT+TGG + chr8.4:60374070-60374089 MS.gene92393:CDS 35.0%
TTAGCCGAGTTTACACTTAA+CGG + chr8.4:60373504-60373523 MS.gene92393:CDS 35.0%
TTGAAAGTGATGAGTAATGG+CGG + chr8.4:60373672-60373691 MS.gene92393:CDS 35.0%
TTGTTGCATATGAACCAGTT+AGG - chr8.4:60373221-60373240 None:intergenic 35.0%
TTTACACTTAACGGTGCAAA+CGG + chr8.4:60373513-60373532 MS.gene92393:CDS 35.0%
AAAAACTGCGAGCATGAACT+TGG - chr8.4:60373786-60373805 None:intergenic 40.0%
AATTAGGGTAACCAAAGCGA+TGG - chr8.4:60373187-60373206 None:intergenic 40.0%
ACTTGTGGGACAAAAAGTGA+TGG - chr8.4:60373889-60373908 None:intergenic 40.0%
ATATCCATCTCCTCAGACAT+TGG - chr8.4:60373939-60373958 None:intergenic 40.0%
CTGAGGAGATGGATATATCT+GGG + chr8.4:60373943-60373962 MS.gene92393:CDS 40.0%
GATCCACAATACTGTTGTAG+TGG + chr8.4:60373732-60373751 MS.gene92393:CDS 40.0%
GGAAAATTCTCCTGTGTTAC+CGG + chr8.4:60373423-60373442 MS.gene92393:CDS 40.0%
GTTCTTGTGGTGAATTTGGT+AGG + chr8.4:60374074-60374093 MS.gene92393:CDS 40.0%
GTTTCCTCCAGTACATTCTA+TGG - chr8.4:60373466-60373485 None:intergenic 40.0%
TCTGAGGAGATGGATATATC+TGG + chr8.4:60373942-60373961 MS.gene92393:CDS 40.0%
TCTTCCACCATAGAATGTAC+TGG + chr8.4:60373456-60373475 MS.gene92393:CDS 40.0%
TGAGGAGATGGATATATCTG+GGG + chr8.4:60373944-60373963 MS.gene92393:CDS 40.0%
TGCACCGTTAAGTGTAAACT+CGG - chr8.4:60373511-60373530 None:intergenic 40.0%
! GGTAACACAGGAGAATTTTC+CGG - chr8.4:60373424-60373443 None:intergenic 40.0%
! GTTTGGCCTGGAATTTTATC+CGG + chr8.4:60373273-60373292 MS.gene92393:CDS 40.0%
AAAGTGATGAGTAATGGCGG+AGG + chr8.4:60373675-60373694 MS.gene92393:CDS 45.0%
AATCTTGAGAGCAGAGGGTA+CGG - chr8.4:60373398-60373417 None:intergenic 45.0%
ACAACACCGTTCTCTACAAC+CGG + chr8.4:60373303-60373322 MS.gene92393:CDS 45.0%
ACGAGTGCAACTACACAGTT+TGG + chr8.4:60373256-60373275 MS.gene92393:CDS 45.0%
ATCTCCAATGTCTGAGGAGA+TGG + chr8.4:60373932-60373951 MS.gene92393:CDS 45.0%
CATGTGGTTCGATCGTCATT+GGG - chr8.4:60373581-60373600 None:intergenic 45.0%
CCACAAGTTCGATCCAATCT+GGG + chr8.4:60373901-60373920 MS.gene92393:CDS 45.0%
CCAGATTGGATCGAACTTGT+GGG - chr8.4:60373903-60373922 None:intergenic 45.0%
CGAGAAATCTCCAATGTCTG+AGG + chr8.4:60373926-60373945 MS.gene92393:CDS 45.0%
CTCATCACTTTCAACTCGGT+TGG - chr8.4:60373666-60373685 None:intergenic 45.0%
GAGATTTCTCGTTCCCAGAT+TGG - chr8.4:60373917-60373936 None:intergenic 45.0%
GAGGAGATGGATATATCTGG+GGG + chr8.4:60373945-60373964 MS.gene92393:CDS 45.0%
GTAAACTCGGCTAATGTAGC+CGG - chr8.4:60373498-60373517 None:intergenic 45.0%
TAAGCGTAGCTATAAGCACG+AGG - chr8.4:60373822-60373841 None:intergenic 45.0%
TCCACCATAGAATGTACTGG+AGG + chr8.4:60373459-60373478 MS.gene92393:CDS 45.0%
TCCTCCAGTACATTCTATGG+TGG - chr8.4:60373463-60373482 None:intergenic 45.0%
! AAAAGCGCATGTGAAGCGTT+TGG + chr8.4:60373708-60373727 MS.gene92393:CDS 45.0%
! AAAGCGCATGTGAAGCGTTT+GGG + chr8.4:60373709-60373728 MS.gene92393:CDS 45.0%
! AACACAGGAGAATTTTCCGG+TGG - chr8.4:60373421-60373440 None:intergenic 45.0%
! TTTCTTCGACGTTAGTCTCG+TGG + chr8.4:60373542-60373561 MS.gene92393:CDS 45.0%
!! GAACTTGGCTTGCATGTATC+TGG - chr8.4:60373771-60373790 None:intergenic 45.0%
!! TGTAGAGAACGGTGTTGTTC+CGG - chr8.4:60373301-60373320 None:intergenic 45.0%
AGCACAATCACCGGTAACAC+AGG - chr8.4:60373436-60373455 None:intergenic 50.0%
ATCGAACCACATGGAGCAAC+CGG + chr8.4:60373588-60373607 MS.gene92393:CDS 50.0%
CATGACAACAGGTTGCATGG+TGG + chr8.4:60373623-60373642 MS.gene92393:CDS 50.0%
CCAATGACGATCGAACCACA+TGG + chr8.4:60373579-60373598 MS.gene92393:CDS 50.0%
CCATGTGGTTCGATCGTCAT+TGG - chr8.4:60373582-60373601 None:intergenic 50.0%
CCCACAAGTTCGATCCAATC+TGG + chr8.4:60373900-60373919 MS.gene92393:CDS 50.0%
CCCAGATTGGATCGAACTTG+TGG - chr8.4:60373904-60373923 None:intergenic 50.0%
CTCTGCTCTCAAGATTCCAC+CGG + chr8.4:60373402-60373421 MS.gene92393:CDS 50.0%
CTGCATGACAACAGGTTGCA+TGG + chr8.4:60373620-60373639 MS.gene92393:CDS 50.0%
GCAAAGCCGGTTGTAGAGAA+CGG - chr8.4:60373312-60373331 None:intergenic 50.0%
GCGCCACTACAACAGTATTG+TGG - chr8.4:60373738-60373757 None:intergenic 50.0%
GGTGGAATCTTGAGAGCAGA+GGG - chr8.4:60373403-60373422 None:intergenic 50.0%
TGCAACTACACAGTTTGGCC+TGG + chr8.4:60373261-60373280 MS.gene92393:CDS 50.0%
! AAGCGCATGTGAAGCGTTTG+GGG + chr8.4:60373710-60373729 MS.gene92393:CDS 50.0%
! GGTGGAAGAAGCACAATCAC+CGG - chr8.4:60373445-60373464 None:intergenic 50.0%
! TTCGACGTTAGTCTCGTGGA+CGG + chr8.4:60373546-60373565 MS.gene92393:CDS 50.0%
AACTCGGCTAATGTAGCCGG+TGG - chr8.4:60373495-60373514 None:intergenic 55.0%
AAGTGGTCAGGCCGTCTATG+GGG + chr8.4:60373372-60373391 MS.gene92393:CDS 55.0%
CAAGTGGTCAGGCCGTCTAT+GGG + chr8.4:60373371-60373390 MS.gene92393:CDS 55.0%
CATAGACGGCCTGACCACTT+GGG - chr8.4:60373372-60373391 None:intergenic 55.0%
CGGTGGAATCTTGAGAGCAG+AGG - chr8.4:60373404-60373423 None:intergenic 55.0%
GGCGGAAACTGCATGACAAC+AGG + chr8.4:60373612-60373631 MS.gene92393:CDS 55.0%
GTGGTTCGATCGTCATTGGG+AGG - chr8.4:60373578-60373597 None:intergenic 55.0%
TGGAGGAAACGCTCAACCAC+CGG + chr8.4:60373476-60373495 MS.gene92393:CDS 55.0%
TGTCATGCAGTTTCCGCCTC+CGG - chr8.4:60373610-60373629 None:intergenic 55.0%
! CCTGGAATTTTATCCGGCGC+CGG + chr8.4:60373279-60373298 MS.gene92393:CDS 55.0%
!! GCAAGGGCATTGGATTCGCT+GGG - chr8.4:60373342-60373361 None:intergenic 55.0%
CCAAGTGGTCAGGCCGTCTA+TGG + chr8.4:60373370-60373389 MS.gene92393:CDS 60.0%
CCATAGACGGCCTGACCACT+TGG - chr8.4:60373373-60373392 None:intergenic 60.0%
GAACCACATGGAGCAACCGG+AGG + chr8.4:60373591-60373610 MS.gene92393:CDS 60.0%
GCCTGACCACTTGGGAGGTA+CGG - chr8.4:60373364-60373383 None:intergenic 60.0%
! ACCACTTGGGAGGTACGGCA+AGG - chr8.4:60373359-60373378 None:intergenic 60.0%
! AGAGGGTACGGCCCCATAGA+CGG - chr8.4:60373386-60373405 None:intergenic 60.0%
! CCACTTGGGAGGTACGGCAA+GGG - chr8.4:60373358-60373377 None:intergenic 60.0%
! CCGGCGCCGGATAAAATTCC+AGG - chr8.4:60373282-60373301 None:intergenic 60.0%
! GCTGGGTTGGAGAGCAAAGC+CGG - chr8.4:60373325-60373344 None:intergenic 60.0%
!! GGCAAGGGCATTGGATTCGC+TGG - chr8.4:60373343-60373362 None:intergenic 60.0%
!! GGGCATTGGATTCGCTGGGT+TGG - chr8.4:60373338-60373357 None:intergenic 60.0%
AGACGGCCTGACCACTTGGG+AGG - chr8.4:60373369-60373388 None:intergenic 65.0%
CCACATGGAGCAACCGGAGG+CGG + chr8.4:60373594-60373613 MS.gene92393:CDS 65.0%
CCCTTGCCGTACCTCCCAAG+TGG + chr8.4:60373355-60373374 MS.gene92393:CDS 65.0%
GCCGTACCTCCCAAGTGGTC+AGG + chr8.4:60373360-60373379 MS.gene92393:CDS 65.0%
! GGGAGGTACGGCAAGGGCAT+TGG - chr8.4:60373352-60373371 None:intergenic 65.0%
!! GAACGGTGTTGTTCCGGCGC+CGG - chr8.4:60373295-60373314 None:intergenic 65.0%
CCGCCTCCGGTTGCTCCATG+TGG - chr8.4:60373597-60373616 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 60373162 60374100 60373162 ID=MS.gene92393
chr8.4 mRNA 60373162 60374100 60373162 ID=MS.gene92393.t1;Parent=MS.gene92393
chr8.4 exon 60373162 60374100 60373162 ID=MS.gene92393.t1.exon1;Parent=MS.gene92393.t1
chr8.4 CDS 60373162 60374100 60373162 ID=cds.MS.gene92393.t1;Parent=MS.gene92393.t1
Gene Sequence

>MS.gene92393

ATGGCTGCTTCAACTCAATATACCATCGCTTTGGTTACCCTAATTCTATTCCAATACCTAACTGGTTCATATGCAACAACATTCACAATTGTTAACGAGTGCAACTACACAGTTTGGCCTGGAATTTTATCCGGCGCCGGAACAACACCGTTCTCTACAACCGGCTTTGCTCTCCAACCCAGCGAATCCAATGCCCTTGCCGTACCTCCCAAGTGGTCAGGCCGTCTATGGGGCCGTACCCTCTGCTCTCAAGATTCCACCGGAAAATTCTCCTGTGTTACCGGTGATTGTGCTTCTTCCACCATAGAATGTACTGGAGGAAACGCTCAACCACCGGCTACATTAGCCGAGTTTACACTTAACGGTGCAAACGGACTAGATTTCTTCGACGTTAGTCTCGTGGACGGTTACAACCTCCCAATGACGATCGAACCACATGGAGCAACCGGAGGCGGAAACTGCATGACAACAGGTTGCATGGTGGATTTGAACGCTGCGTGTCCAACCGAGTTGAAAGTGATGAGTAATGGCGGAGGTGTAGCGTGTAAAAGCGCATGTGAAGCGTTTGGGGATCCACAATACTGTTGTAGTGGCGCTTACGCTTCTCCAGATACATGCAAGCCAAGTTCATGCTCGCAGTTTTTCAAGAGTGCTTGTCCTCGTGCTTATAGCTACGCTTATGATGATGGAACAAGCACTTTTACATGTGATTCTGCTGATTATACCATCACTTTTTGTCCCACAAGTTCGATCCAATCTGGGAACGAGAAATCTCCAATGTCTGAGGAGATGGATATATCTGGGGGACATGTGAACTTCACAGCCAACAATGTTATGATTGTTGTTATTATTTCTTTTGTTTTTTGTTTTTTGTTTTTTTTTTTGAAGGATTATTTCTTTTGTTTTGATGTTGTTCTTGTGGTGAATTTGGTAGGCTAG
Protein sequence

>MS.gene92393.t1

MAASTQYTIALVTLILFQYLTGSYATTFTIVNECNYTVWPGILSGAGTTPFSTTGFALQPSESNALAVPPKWSGRLWGRTLCSQDSTGKFSCVTGDCASSTIECTGGNAQPPATLAEFTLNGANGLDFFDVSLVDGYNLPMTIEPHGATGGGNCMTTGCMVDLNAACPTELKVMSNGGGVACKSACEAFGDPQYCCSGAYASPDTCKPSSCSQFFKSACPRAYSYAYDDGTSTFTCDSADYTITFCPTSSIQSGNEKSPMSEEMDISGGHVNFTANNVMIVVIISFVFCFLFFFLKDYFFCFDVVLVVNLVG