AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073301


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073301.t1 MTR_4g127640 96.000 100 4 0 23 122 183 282 1.64e-54 172
MS.gene073301.t1 MTR_4g127640 96.000 100 4 0 23 122 201 300 2.16e-54 172
MS.gene073301.t1 MTR_4g127640 96.000 100 4 0 23 122 192 291 2.32e-54 172
MS.gene073301.t1 MTR_4g007420 60.000 100 33 3 23 122 192 284 6.86e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073301.t1 AT5G57280 55.000 100 43 2 23 122 192 289 1.13e-27 103

Find 25 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTTTCTCTGCTTCTTAA+AGG 0.231198 8.1:-5199960 None:intergenic
AGAAAAGAGTTTCTTGTTCT+CGG 0.339903 8.1:+5199336 MS.gene073301:CDS
GAGCATGGATTTCCACTAAC+TGG 0.363568 8.1:+5199282 MS.gene073301:CDS
TTCTTTCTCTGCTTCTTAAA+GGG 0.367013 8.1:-5199959 None:intergenic
GACAATTGTTCTCCAGTTAG+TGG 0.397912 8.1:-5199294 None:intergenic
GAGTTTCTTGTTCTCGGATG+TGG 0.418174 8.1:+5199342 MS.gene073301:CDS
TCGTTTCCGACCAGTGTACT+TGG 0.444474 8.1:-5200065 None:intergenic
TGCAGGTGCGTTTGTCAGAC+AGG 0.445651 8.1:+5199930 MS.gene073301:intron
TTCATCTTCGCTCTCTTCAT+CGG 0.463844 8.1:-5199424 None:intergenic
AAGGATCAAATGAGAAGAAG+AGG 0.492941 8.1:+5200022 MS.gene073301:CDS
TTGCATAGCCTTCTTGAGCA+TGG 0.504441 8.1:+5199267 MS.gene073301:intron
TAATAAGAGCAGCAAGGGAA+AGG 0.520324 8.1:+5199982 MS.gene073301:CDS
TACACTGGTCGGAAACGAAA+GGG 0.531240 8.1:+5200070 MS.gene073301:CDS
AAGAATAATAAGAGCAGCAA+GGG 0.533361 8.1:+5199977 MS.gene073301:CDS
AGAGCAGCAAGGGAAAGGAA+TGG 0.589939 8.1:+5199987 MS.gene073301:CDS
GGAATGGATACTGAGGAAGA+AGG 0.594390 8.1:+5200003 MS.gene073301:CDS
GTGGAAATCCATGCTCAAGA+AGG 0.597891 8.1:-5199275 None:intergenic
CCAGTGTACTTGGTATCTAG+AGG 0.603613 8.1:-5200055 None:intergenic
GTACACTGGTCGGAAACGAA+AGG 0.604131 8.1:+5200069 MS.gene073301:CDS
AAAGAATAATAAGAGCAGCA+AGG 0.610322 8.1:+5199976 MS.gene073301:CDS
TAGATACCAAGTACACTGGT+CGG 0.625666 8.1:+5200059 MS.gene073301:CDS
ACGAAAGCATCCTTGTCCAA+AGG 0.634791 8.1:+5199375 MS.gene073301:CDS
CAGTGTACTTGGTATCTAGA+GGG 0.642930 8.1:-5200054 None:intergenic
AGGGAAAGGAATGGATACTG+AGG 0.667986 8.1:+5199996 MS.gene073301:CDS
CCTCTAGATACCAAGTACAC+TGG 0.682621 8.1:+5200055 MS.gene073301:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTATTTTTCTTATAAG+TGG + chr8.1:5199743-5199762 MS.gene073301:intron 10.0%
!! ATAAAGATAAAAGTAAAAGA+TGG + chr8.1:5198915-5198934 MS.gene073301:intron 15.0%
!! ATCACTATCTATTAATAAAA+TGG + chr8.1:5199606-5199625 MS.gene073301:intron 15.0%
!! CATGCATATAATTTAAATTA+TGG + chr8.1:5199704-5199723 MS.gene073301:intron 15.0%
!!! CATATCTGAAATAAATTTAT+GGG - chr8.1:5199826-5199845 None:intergenic 15.0%
!!! TCATATCTGAAATAAATTTA+TGG - chr8.1:5199827-5199846 None:intergenic 15.0%
!!! TTATTGTTACTTTCATTTTT+AGG - chr8.1:5198853-5198872 None:intergenic 15.0%
!! GAAAATTACTCTGATTAAAT+TGG + chr8.1:5198623-5198642 MS.gene073301:intron 20.0%
!! TAAGTTGTAACAAAATGAAT+AGG - chr8.1:5199552-5199571 None:intergenic 20.0%
!!! TAATTTTCTTACCAAATCAA+GGG - chr8.1:5198611-5198630 None:intergenic 20.0%
!!! TTTAGTTTAAAAAATACTGC+AGG + chr8.1:5199913-5199932 MS.gene073301:intron 20.0%
! AAGTAAATTGCACTCTTTAA+AGG - chr8.1:5199640-5199659 None:intergenic 25.0%
! AATAAAATAAGAGTGAGAAG+AGG + chr8.1:5198984-5199003 MS.gene073301:intron 25.0%
! AATATAAGCTCGATACAAAA+AGG - chr8.1:5199895-5199914 None:intergenic 25.0%
! ATAAAATAAGAGTGAGAAGA+GGG + chr8.1:5198985-5199004 MS.gene073301:intron 25.0%
! TCTGATTAAATTGGAATTGA+AGG + chr8.1:5198632-5198651 MS.gene073301:intron 25.0%
!! GTAATTTTCTTACCAAATCA+AGG - chr8.1:5198612-5198631 None:intergenic 25.0%
!!! TGTTTTTGTTTTGTTCGATT+TGG + chr8.1:5199212-5199231 MS.gene073301:intron 25.0%
AAAGAATAATAAGAGCAGCA+AGG + chr8.1:5199976-5199995 MS.gene073301:CDS 30.0%
AAGAATAATAAGAGCAGCAA+GGG + chr8.1:5199977-5199996 MS.gene073301:CDS 30.0%
AGAAAAGAGTTTCTTGTTCT+CGG + chr8.1:5199336-5199355 MS.gene073301:CDS 30.0%
AGAGTGCAATTTACTTTACA+TGG + chr8.1:5199644-5199663 MS.gene073301:intron 30.0%
ATTCTTTCTCTGCTTCTTAA+AGG - chr8.1:5199963-5199982 None:intergenic 30.0%
GATTAAATTGGAATTGAAGG+TGG + chr8.1:5198635-5198654 MS.gene073301:intron 30.0%
TTCTTTCTCTGCTTCTTAAA+GGG - chr8.1:5199962-5199981 None:intergenic 30.0%
! CAGAATATTCTCTGTCTTTT+CGG - chr8.1:5199066-5199085 None:intergenic 30.0%
! TGTCTTTTCGGTGAAAATTT+CGG - chr8.1:5199054-5199073 None:intergenic 30.0%
! TTCATCTTCAGTTTTACCTT+TGG - chr8.1:5199394-5199413 None:intergenic 30.0%
AAAGAAAGAAAAAAGGACCC+TGG + chr8.1:5198745-5198764 MS.gene073301:intron 35.0%
AAGGATCAAATGAGAAGAAG+AGG + chr8.1:5200022-5200041 MS.gene073301:CDS 35.0%
AATGCAACTCTCACTTAACA+TGG - chr8.1:5199788-5199807 None:intergenic 35.0%
ATAAGCTCGATACAAAAAGG+AGG - chr8.1:5199892-5199911 None:intergenic 35.0%
CGTGGAGAAAGAAAGAAAAA+AGG + chr8.1:5198738-5198757 MS.gene073301:intron 35.0%
GAATAGGTAGTGATCTTTGA+AGG - chr8.1:5199536-5199555 None:intergenic 35.0%
GATCCTTAGGTCAAAAATTG+GGG + chr8.1:5199108-5199127 MS.gene073301:intron 35.0%
GGATCCTTAGGTCAAAAATT+GGG + chr8.1:5199107-5199126 MS.gene073301:intron 35.0%
GGTATGACATGTAATGTATC+TGG + chr8.1:5199129-5199148 MS.gene073301:intron 35.0%
TAAGCTCGATACAAAAAGGA+GGG - chr8.1:5199891-5199910 None:intergenic 35.0%
TAAGTGAGAGTTGCATTTCA+AGG + chr8.1:5199791-5199810 MS.gene073301:intron 35.0%
TGGAATTGAAGGTGGTTAAA+TGG + chr8.1:5198643-5198662 MS.gene073301:intron 35.0%
TGGATCCTTAGGTCAAAAAT+TGG + chr8.1:5199106-5199125 MS.gene073301:intron 35.0%
! AGGTGGTTAAATGGTGTTAA+TGG + chr8.1:5198652-5198671 MS.gene073301:intron 35.0%
! ATACCCCAATTTTTGACCTA+AGG - chr8.1:5199114-5199133 None:intergenic 35.0%
! GTTAAATGGTGTTAATGGTG+AGG + chr8.1:5198657-5198676 MS.gene073301:intron 35.0%
! TTCAGTTTTACCTTTGGACA+AGG - chr8.1:5199388-5199407 None:intergenic 35.0%
ATGAGTCAAAATCCAAGTCC+AGG - chr8.1:5198766-5198785 None:intergenic 40.0%
CAGTGTACTTGGTATCTAGA+GGG - chr8.1:5200057-5200076 None:intergenic 40.0%
CCTGAATCGGAACAAAAATG+AGG + chr8.1:5199008-5199027 MS.gene073301:intron 40.0%
GAAACATTCATCGTGCCTAT+TGG - chr8.1:5199501-5199520 None:intergenic 40.0%
GACAATTGTTCTCCAGTTAG+TGG - chr8.1:5199297-5199316 None:intergenic 40.0%
TAATAAGAGCAGCAAGGGAA+AGG + chr8.1:5199982-5200001 MS.gene073301:CDS 40.0%
TAGATACCAAGTACACTGGT+CGG + chr8.1:5200059-5200078 MS.gene073301:CDS 40.0%
TCGGTTCAGGAATTTCCAAT+AGG + chr8.1:5199483-5199502 MS.gene073301:intron 40.0%
TCTCACCACATCTGTGTATT+CGG + chr8.1:5199464-5199483 MS.gene073301:intron 40.0%
TGAGTCAAAATCCAAGTCCA+GGG - chr8.1:5198765-5198784 None:intergenic 40.0%
TTCATCTTCGCTCTCTTCAT+CGG - chr8.1:5199427-5199446 None:intergenic 40.0%
! ACGCGTTTGGTGATTGTTAT+GGG + chr8.1:5198680-5198699 MS.gene073301:intron 40.0%
!! CCTCATTTTTGTTCCGATTC+AGG - chr8.1:5199011-5199030 None:intergenic 40.0%
!!! GGACTTGGATTTTGACTCAT+TGG + chr8.1:5198766-5198785 MS.gene073301:intron 40.0%
ACATGTGTCAGTGGATCCTT+AGG + chr8.1:5199095-5199114 MS.gene073301:intron 45.0%
AGAAAAAAGGACCCTGGACT+TGG + chr8.1:5198751-5198770 MS.gene073301:intron 45.0%
AGGGAAAGGAATGGATACTG+AGG + chr8.1:5199996-5200015 MS.gene073301:CDS 45.0%
CACATCTGTGTATTCGGTTC+AGG + chr8.1:5199470-5199489 MS.gene073301:intron 45.0%
CAGAGAATATTCTGTGCCAG+AGG + chr8.1:5199072-5199091 MS.gene073301:intron 45.0%
CCAGTGTACTTGGTATCTAG+AGG - chr8.1:5200058-5200077 None:intergenic 45.0%
CCTCTAGATACCAAGTACAC+TGG + chr8.1:5200055-5200074 MS.gene073301:CDS 45.0%
CTGAACCGAATACACAGATG+TGG - chr8.1:5199472-5199491 None:intergenic 45.0%
GAGCATGGATTTCCACTAAC+TGG + chr8.1:5199282-5199301 MS.gene073301:CDS 45.0%
GAGTTTCTTGTTCTCGGATG+TGG + chr8.1:5199342-5199361 MS.gene073301:CDS 45.0%
GCATTGCACAATCAGTGTCA+AGG - chr8.1:5198719-5198738 None:intergenic 45.0%
GGAATGGATACTGAGGAAGA+AGG + chr8.1:5200003-5200022 MS.gene073301:CDS 45.0%
GTGGAAATCCATGCTCAAGA+AGG - chr8.1:5199278-5199297 None:intergenic 45.0%
TACACTGGTCGGAAACGAAA+GGG + chr8.1:5200070-5200089 MS.gene073301:CDS 45.0%
TTGCATAGCCTTCTTGAGCA+TGG + chr8.1:5199267-5199286 MS.gene073301:intron 45.0%
! GACGCGTTTGGTGATTGTTA+TGG + chr8.1:5198679-5198698 MS.gene073301:intron 45.0%
!! ACGAAAGCATCCTTGTCCAA+AGG + chr8.1:5199375-5199394 MS.gene073301:CDS 45.0%
AGAGCAGCAAGGGAAAGGAA+TGG + chr8.1:5199987-5200006 MS.gene073301:CDS 50.0%
AGTGAGAAGAGGGCCTGAAT+CGG + chr8.1:5198995-5199014 MS.gene073301:intron 50.0%
ATCCACTGACACATGTCCTC+TGG - chr8.1:5199091-5199110 None:intergenic 50.0%
CGGTGAAAATTTCGGCAGAG+AGG - chr8.1:5199046-5199065 None:intergenic 50.0%
GTACACTGGTCGGAAACGAA+AGG + chr8.1:5200069-5200088 MS.gene073301:CDS 50.0%
GTTAATGGTGAGGACGCGTT+TGG + chr8.1:5198667-5198686 MS.gene073301:intron 50.0%
TCGTTTCCGACCAGTGTACT+TGG - chr8.1:5200068-5200087 None:intergenic 50.0%
! GACACTGATTGTGCAATGCG+TGG + chr8.1:5198720-5198739 MS.gene073301:intron 50.0%
TGCAGGTGCGTTTGTCAGAC+AGG + chr8.1:5199930-5199949 MS.gene073301:intron 55.0%
TGCCAGAGGACATGTGTCAG+TGG + chr8.1:5199086-5199105 MS.gene073301:intron 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 5198602 5200102 5198602 ID=MS.gene073301
chr8.1 mRNA 5198602 5200102 5198602 ID=MS.gene073301.t1;Parent=MS.gene073301
chr8.1 exon 5198602 5198618 5198602 ID=MS.gene073301.t1.exon1;Parent=MS.gene073301.t1
chr8.1 CDS 5198602 5198618 5198602 ID=cds.MS.gene073301.t1;Parent=MS.gene073301.t1
chr8.1 exon 5199275 5199458 5199275 ID=MS.gene073301.t1.exon2;Parent=MS.gene073301.t1
chr8.1 CDS 5199275 5199458 5199275 ID=cds.MS.gene073301.t1;Parent=MS.gene073301.t1
chr8.1 exon 5199935 5200102 5199935 ID=MS.gene073301.t1.exon3;Parent=MS.gene073301.t1
chr8.1 CDS 5199935 5200102 5199935 ID=cds.MS.gene073301.t1;Parent=MS.gene073301.t1
Gene Sequence

>MS.gene073301

ATGCATCCCTTGATTTGGTAAGAAAATTACTCTGATTAAATTGGAATTGAAGGTGGTTAAATGGTGTTAATGGTGAGGACGCGTTTGGTGATTGTTATGGGAAGAAAGAGTAGACCTTGACACTGATTGTGCAATGCGTGGAGAAAGAAAGAAAAAAGGACCCTGGACTTGGATTTTGACTCATTGGACACTTTGTTTTTTTTGACATTTTTTTACTATATAAAAAAAAGAAAAAAAAGAACATAAGACCTAAAAATGAAAGTAACAATAATAAAAAGTAAAAGATAAAAGTGAAACTAAAATAAAAAATATAATAAAGATAAAAGTAAAAGATGGAAAATTAAAAAGTAAAAATAAAAACTAAAAGACTAATAAATAATAAAATAAAATAAGAGTGAGAAGAGGGCCTGAATCGGAACAAAAATGAGGTATTTTAAAATACCTCTCTGCCGAAATTTTCACCGAAAAGACAGAGAATATTCTGTGCCAGAGGACATGTGTCAGTGGATCCTTAGGTCAAAAATTGGGGTATGACATGTAATGTATCTGGTCATACATTGTTGATTATGTAACATGTTTAAAAGAAAATTTACGATAGTTCATTGTTACGTGTTTTTGTTTTGTTCGATTTGGTTTTTAGCATTGCTTTATTTTGAAAATAATTGTTGCATAGCCTTCTTGAGCATGGATTTCCACTAACTGGAGAACAATTGTCTTATGTTAGTTCTAAGAAGAGAAAAGAGTTTCTTGTTCTCGGATGTGGTCAACTATCAACGAAAGCATCCTTGTCCAAAGGTAAAACTGAAGATGAAGAGATGTTATCCGATGAAGAGAGCGAAGATGAAGAAAATCAGACAGTAAGTCTCACCACATCTGTGTATTCGGTTCAGGAATTTCCAATAGGCACGATGAATGTTTCTTTAAATTTCATCCTTCAAAGATCACTACCTATTCATTTTGTTACAACTTATGCTTTTATTTTGATTAGTTAGATTTATTTAAAGATCACTATCTATTAATAAAATGGACATTTCTCCTTTAAAGAGTGCAATTTACTTTACATGGTAATGTGAGAAATCTCAATTGCTGATGAAAAAAATAACATGCATATAATTTAAATTATGGATGTGTTAATATGTCAATTATTATTTTTCTTATAAGTGGTTAAGATAAGATGTCTTTACCATGTTAAGTGAGAGTTGCATTTCAAGGTGTTGAATTCCCATAAATTTATTTCAGATATGAAAAATGCAAAATATGATGATACATTGCTTATGTAGATGCAGCCCTCCTTTTTGTATCGAGCTTATATTTAGTTTAAAAAATACTGCAGGTGCGTTTGTCAGACAGGCATAGACCCTTTAAGAAGCAGAGAAAGAATAATAAGAGCAGCAAGGGAAAGGAATGGATACTGAGGAAGAAGGATCAAATGAGAAGAAGAGGAAACGATGTCCCTCTAGATACCAAGTACACTGGTCGGAAACGAAAGGGCCGCTTTTGA

Protein sequence

>MS.gene073301.t1

MHPLICLLEHGFPLTGEQLSYVSSKKRKEFLVLGCGQLSTKASLSKGKTEDEEMLSDEESEDEENQTVRLSDRHRPFKKQRKNNKSSKGKEWILRKKDQMRRRGNDVPLDTKYTGRKRKGRF