AlfalfaGEDB | MS.gene20280

M. sativa cultivar XinJiangDaYe / MS.gene20280

KEGG: K17428
GO: GO:0000313 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005761 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF06984.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20280.t1	XP_003611613.2	97.8	137	2	1	1	136	1	137	7.30E-67	263.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20280.t1	Q8K2Y7	50.6	89	43	1	29	116	63	151	2.1e-14	80.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20280.t1	G7K3V1	97.8	137	2	1	1	136	1	137	5.2e-67	263.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene20280	0.842706	2.20E-58	-1.69E-46
MS.gene051330	MS.gene20280	0.805192	1.50E-49	-1.69E-46
MS.gene051756	MS.gene20280	0.801497	8.76E-49	-1.69E-46
MS.gene051903	MS.gene20280	0.821554	3.80E-53	-1.69E-46
MS.gene052202	MS.gene20280	0.802793	4.74E-49	-1.69E-46
MS.gene053109	MS.gene20280	0.833819	4.28E-56	-1.69E-46
MS.gene056601	MS.gene20280	0.818874	1.56E-52	-1.69E-46
MS.gene056631	MS.gene20280	0.836651	8.26E-57	-1.69E-46
MS.gene057094	MS.gene20280	0.804993	1.65E-49	-1.69E-46
MS.gene057234	MS.gene20280	0.817631	2.98E-52	-1.69E-46
MS.gene058238	MS.gene20280	0.853662	2.06E-61	-1.69E-46
MS.gene058906	MS.gene20280	0.816289	5.97E-52	-1.69E-46
MS.gene059898	MS.gene20280	0.80709	5.99E-50	-1.69E-46
MS.gene06001	MS.gene20280	0.841876	3.64E-58	-1.69E-46
MS.gene061145	MS.gene20280	0.821128	4.76E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene20280	MS.gene001687	PPI
MS.gene20280	MS.gene005865	PPI
MS.gene20280	MS.gene066059	PPI
MS.gene20280	MS.gene21110	PPI
MS.gene31468	MS.gene20280	PPI
MS.gene20280	MS.gene036564	PPI
MS.gene20280	MS.gene026968	PPI
MS.gene20280	MS.gene60861	PPI
MS.gene34167	MS.gene20280	PPI
MS.gene21111	MS.gene20280	PPI
MS.gene20280	MS.gene028903	PPI
MS.gene20280	MS.gene21227	PPI
MS.gene20280	MS.gene055808	PPI
MS.gene20280	MS.gene035256	PPI
MS.gene20280	MS.gene20938	PPI
MS.gene60589	MS.gene20280	PPI
MS.gene20280	MS.gene82915	PPI
MS.gene005399	MS.gene20280	PPI
MS.gene20280	MS.gene043435	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20280.t1	MTR_5g015850	97.810	137	2	1	1	136	1	137	6.36e-97	275

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20280.t1	AT1G07830	71.528	144	33	3	1	136	1	144	4.39e-67	199

Find 25 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGAATTGCGTCTGAAATCT+TGG	0.295639	5.1:-8239202	MS.gene20280:CDS
CCCTCTTCCGGAGTTCTTTG+AGG	0.397007	5.1:-8239417	MS.gene20280:CDS
GAGATGAAGAAAATGATAAA+TGG	0.401349	5.1:-8238745	MS.gene20280:CDS
TTAGGTAAGCGCTCCGGGTT+AGG	0.401390	5.1:+8239086	None:intergenic
GTTGTGGTATGTGTTGTTAA+AGG	0.420053	5.1:-8239165	MS.gene20280:CDS
AACGAGACACCAGTTGTCTA+TGG	0.421926	5.1:-8239383	MS.gene20280:intron
TGAATTGCGTCTGAAATCTT+GGG	0.436291	5.1:-8239201	MS.gene20280:CDS
TCAGCAGACCTCCTCGCGTC+TGG	0.436837	5.1:+8238766	None:intergenic
TGCTGTTAGAAACCCTCTTC+CGG	0.440920	5.1:-8239429	MS.gene20280:CDS
ATACCTTAGGTAAGCGCTCC+GGG	0.470522	5.1:+8239081	None:intergenic
GAACTTGCGCTTTCCTAACC+CGG	0.481233	5.1:-8239099	MS.gene20280:CDS
GCCTCAAAGAACTCCGGAAG+AGG	0.493349	5.1:+8239416	None:intergenic
TATTTCATACCATAGACAAC+TGG	0.503485	5.1:+8239374	None:intergenic
AATACCTTAGGTAAGCGCTC+CGG	0.514447	5.1:+8239080	None:intergenic
AAACAGTATTCCACTACAGC+TGG	0.543067	5.1:-8239452	MS.gene20280:CDS
ACACATACCACAACTTATGA+AGG	0.545630	5.1:+8239174	None:intergenic
TTTGTTAGGTCGGAGTTGGA+AGG	0.551769	5.1:-8239228	MS.gene20280:intron
GGGATGACCTTCATAAGTTG+TGG	0.556409	5.1:-8239181	MS.gene20280:CDS
TCTAACAGCACCAGCTGTAG+TGG	0.557909	5.1:+8239442	None:intergenic
TAACCCGGAGCGCTTACCTA+AGG	0.570629	5.1:-8239084	MS.gene20280:intron
CTGTCTGCCTCAAAGAACTC+CGG	0.578032	5.1:+8239410	None:intergenic
CCTCAAAGAACTCCGGAAGA+GGG	0.599992	5.1:+8239417	None:intergenic
TTGAAGAACCAGACGCGAGG+AGG	0.633013	5.1:-8238774	MS.gene20280:CDS
CAGTAAGCACATGCTTGATG+CGG	0.642610	5.1:+8238806	None:intergenic
CGATTGAAGAACCAGACGCG+AGG	0.687088	5.1:-8238777	MS.gene20280:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATGTTAAATTTCGTAT+GGG	-	chr5.1:8239337-8239356	MS.gene20280:intron	15.0%
!!	GTAAAATGTTAAATTTCGTA+TGG	-	chr5.1:8239336-8239355	MS.gene20280:intron	20.0%
!!!	ATAGTTTTACCATTTTTGTT+AGG	-	chr5.1:8239002-8239021	MS.gene20280:intron	20.0%
!!!	GATTTAATTTATGTGAAATC+AGG	-	chr5.1:8239399-8239418	MS.gene20280:CDS	20.0%
!	AAAAAGTGAAGAAATACCTT+AGG	+	chr5.1:8239179-8239198	None:intergenic	25.0%
!	GAGATGAAGAAAATGATAAA+TGG	-	chr5.1:8239499-8239518	MS.gene20280:CDS	25.0%
!	TATAAGAAAACAAAGTGATG+AGG	+	chr5.1:8238985-8239004	None:intergenic	25.0%
!!!	ATTTTTTTGTACCTAATGTG+TGG	+	chr5.1:8238953-8238972	None:intergenic	25.0%
!!!	TTTTACCATTTTTGTTAGGT+CGG	-	chr5.1:8239006-8239025	MS.gene20280:intron	25.0%
	AGTTATGTGGACATTGATAT+AGG	-	chr5.1:8239239-8239258	MS.gene20280:intron	30.0%
	ATCAATGTCCACATAACTAA+GGG	+	chr5.1:8239237-8239256	None:intergenic	30.0%
	ATGTTAAATTTCGTATGGGT+TGG	-	chr5.1:8239341-8239360	MS.gene20280:intron	30.0%
	CCTATTCTATTGAAAAGAAG+AGG	+	chr5.1:8238900-8238919	None:intergenic	30.0%
	TATCAATGTCCACATAACTA+AGG	+	chr5.1:8239238-8239257	None:intergenic	30.0%
	TATTTCATACCATAGACAAC+TGG	+	chr5.1:8238873-8238892	None:intergenic	30.0%
	TGTTAAATTTCGTATGGGTT+GGG	-	chr5.1:8239342-8239361	MS.gene20280:intron	30.0%
	TTTCATTATAGCCACACATT+AGG	-	chr5.1:8238939-8238958	MS.gene20280:intron	30.0%
!	CCTCTTCTTTTCAATAGAAT+AGG	-	chr5.1:8238897-8238916	MS.gene20280:intron	30.0%
!	TTTTGTTACCCTTAGTTATG+TGG	-	chr5.1:8239226-8239245	MS.gene20280:intron	30.0%
!!!	ATTTCTTCACTTTTTACACC+TGG	-	chr5.1:8239184-8239203	MS.gene20280:CDS	30.0%
	ACACATACCACAACTTATGA+AGG	+	chr5.1:8239073-8239092	None:intergenic	35.0%
	ATTGATATAGGAAATCGCAG+TGG	-	chr5.1:8239251-8239270	MS.gene20280:intron	35.0%
!	GTTAAATTTCGTATGGGTTG+GGG	-	chr5.1:8239343-8239362	MS.gene20280:intron	35.0%
!	GTTGTGGTATGTGTTGTTAA+AGG	-	chr5.1:8239079-8239098	MS.gene20280:intron	35.0%
!	TGAATTGCGTCTGAAATCTT+GGG	-	chr5.1:8239043-8239062	MS.gene20280:intron	35.0%
!!	TTAAATTTCGTATGGGTTGG+GGG	-	chr5.1:8239344-8239363	MS.gene20280:intron	35.0%
!!!	AGTGGAATACTGTTTTGATC+TGG	+	chr5.1:8238787-8238806	None:intergenic	35.0%
	AAACAGTATTCCACTACAGC+TGG	-	chr5.1:8238792-8238811	MS.gene20280:CDS	40.0%
	CAACTCCGACCTAACAAAAA+TGG	+	chr5.1:8239014-8239033	None:intergenic	40.0%
!	CATTTTTGTTAGGTCGGAGT+TGG	-	chr5.1:8239012-8239031	MS.gene20280:intron	40.0%
!	CTGAATTGCGTCTGAAATCT+TGG	-	chr5.1:8239042-8239061	MS.gene20280:intron	40.0%
!	GTGGTGCTTTTATCAACGTT+TGG	-	chr5.1:8239273-8239292	MS.gene20280:intron	40.0%
!!	TAAATTTCGTATGGGTTGGG+GGG	-	chr5.1:8239345-8239364	MS.gene20280:intron	40.0%
	AACGAGACACCAGTTGTCTA+TGG	-	chr5.1:8238861-8238880	MS.gene20280:intron	45.0%
	AATACCTTAGGTAAGCGCTC+CGG	+	chr5.1:8239167-8239186	None:intergenic	45.0%
	CAGTAAGCACATGCTTGATG+CGG	+	chr5.1:8239441-8239460	None:intergenic	45.0%
	GATATAGGAAATCGCAGTGG+TGG	-	chr5.1:8239254-8239273	MS.gene20280:intron	45.0%
	GGGATGACCTTCATAAGTTG+TGG	-	chr5.1:8239063-8239082	MS.gene20280:intron	45.0%
	TGCTGTTAGAAACCCTCTTC+CGG	-	chr5.1:8238815-8238834	MS.gene20280:CDS	45.0%
	TTTGTTAGGTCGGAGTTGGA+AGG	-	chr5.1:8239016-8239035	MS.gene20280:intron	45.0%
	ATACCTTAGGTAAGCGCTCC+GGG	+	chr5.1:8239166-8239185	None:intergenic	50.0%
	CAACACGCGTAAATCAGACC+AGG	+	chr5.1:8239205-8239224	None:intergenic	50.0%
	CCTCAAAGAACTCCGGAAGA+GGG	+	chr5.1:8238830-8238849	None:intergenic	50.0%
	CTGTCTGCCTCAAAGAACTC+CGG	+	chr5.1:8238837-8238856	None:intergenic	50.0%
	GAACTTGCGCTTTCCTAACC+CGG	-	chr5.1:8239145-8239164	MS.gene20280:CDS	50.0%
	TCTAACAGCACCAGCTGTAG+TGG	+	chr5.1:8238805-8238824	None:intergenic	50.0%
	CCCTCTTCCGGAGTTCTTTG+AGG	-	chr5.1:8238827-8238846	MS.gene20280:intron	55.0%
	CGATTGAAGAACCAGACGCG+AGG	-	chr5.1:8239467-8239486	MS.gene20280:CDS	55.0%
	GCCTCAAAGAACTCCGGAAG+AGG	+	chr5.1:8238831-8238850	None:intergenic	55.0%
	GGTTGGGGGGAAACTGTTCA+CGG	-	chr5.1:8239358-8239377	MS.gene20280:intron	55.0%
	TTAGGTAAGCGCTCCGGGTT+AGG	+	chr5.1:8239161-8239180	None:intergenic	55.0%
	TTGAAGAACCAGACGCGAGG+AGG	-	chr5.1:8239470-8239489	MS.gene20280:CDS	55.0%
!	TAACCCGGAGCGCTTACCTA+AGG	-	chr5.1:8239160-8239179	MS.gene20280:CDS	55.0%
	TCAGCAGACCTCCTCGCGTC+TGG	+	chr5.1:8239481-8239500	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	8238738	8239528	8238738	ID=MS.gene20280
chr5.1	mRNA	8238738	8239528	8238738	ID=MS.gene20280.t1;Parent=MS.gene20280
chr5.1	exon	8239384	8239528	8239384	ID=MS.gene20280.t1.exon1;Parent=MS.gene20280.t1
chr5.1	CDS	8239384	8239528	8239384	ID=cds.MS.gene20280.t1;Parent=MS.gene20280.t1
chr5.1	exon	8239085	8239242	8239085	ID=MS.gene20280.t1.exon2;Parent=MS.gene20280.t1
chr5.1	CDS	8239085	8239242	8239085	ID=cds.MS.gene20280.t1;Parent=MS.gene20280.t1
chr5.1	exon	8238738	8238845	8238738	ID=MS.gene20280.t1.exon3;Parent=MS.gene20280.t1
chr5.1	CDS	8238738	8238845	8238738	ID=cds.MS.gene20280.t1;Parent=MS.gene20280.t1

Gene Sequence

>MS.gene20280

ATGTTTTTGGCAAGAGCATTTCGACAATCATTCTTTGCTGCTGCTGCCAGATCAAAACAGTATTCCACTACAGCTGGTGCTGTTAGAAACCCTCTTCCGGAGTTCTTTGAGGCAGACAGAGATAACGAGACACCAGTTGTCTATGGTATGAAATATGCACCTCTTCTTTTCAATAGAATAGGAATTAAAAATCTCACTGATTTTCATTATAGCCACACATTAGGTACAAAAAAATTATAATTTTCCTCATCACTTTGTTTTCTTATAGTTTTACCATTTTTGTTAGGTCGGAGTTGGAAGGCTTCTGAATTGCGTCTGAAATCTTGGGATGACCTTCATAAGTTGTGGTATGTGTTGTTAAAGGAAAAGAATATGTTGATGACTCAACGACAAATGCTTCAAGCTCAGAACTTGCGCTTTCCTAACCCGGAGCGCTTACCTAAGGTATTTCTTCACTTTTTACACCTGGTCTGATTTACGCGTGTTGTTTTTGTTACCCTTAGTTATGTGGACATTGATATAGGAAATCGCAGTGGTGGTGCTTTTATCAACGTTTGGTGAACACTTTTTGTTTTAGAACATACGAGAGAAATTGAATGTAAAATGTTAAATTTCGTATGGGTTGGGGGGAAACTGTTCACGGTTAACAATGATTTTTGTTGATTTAATTTATGTGAAATCAGGTGAGAAAGTCAATGTGCCGCATCAAGCATGTGCTTACTGAGAGAGCGATTGAAGAACCAGACGCGAGGAGGTCTGCTGAGATGAAGAAAATGATAAATGGTCTTTGA

Protein sequence

>MS.gene20280.t1

MFLARAFRQSFFAAAARSKQYSTTAGAVRNPLPEFFEADRDNETPVVYGRSWKASELRLKSWDDLHKLWYVLLKEKNMLMTQRQMLQAQNLRFPNPERLPKVRKSMCRIKHVLTERAIEEPDARRSAEMKKMINGL