AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005399

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005399.t1 MTR_1g031890 98.214 280 5 0 1 280 1 280 0.0 553
MS.gene005399.t1 MTR_1g085490 97.857 280 6 0 1 280 1 280 0.0 551
MS.gene005399.t1 MTR_1g031890 98.195 277 5 0 1 277 1 277 0.0 548
MS.gene005399.t1 MTR_4g118060 97.143 280 8 0 1 280 1 280 0.0 546
MS.gene005399.t1 MTR_1g031890 97.938 194 4 0 1 194 1 194 2.58e-137 385
MS.gene005399.t1 MTR_1g110750 31.977 172 102 7 74 233 78 246 9.41e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005399.t1 AT5G64670 73.646 277 56 5 1 268 1 269 3.56e-138 391
MS.gene005399.t1 AT3G25920 30.667 225 128 11 5 211 19 233 7.69e-13 67.4

Find 91 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACAATAAGTTAGGCTTTC+GGG 0.235237 1.1:+63295815 MS.gene005399:CDS
CCCCAACTTTGAACCTTTCC+TGG 0.235844 1.1:-63293789 None:intergenic
GCTCTAAAACCTTGAAAATC+TGG 0.242722 1.1:-63293641 None:intergenic
ACCTGAAAAGTGAGGCTAAA+TGG 0.251841 1.1:-63293869 None:intergenic
GGAGGAGGTCTAGCTGCTTT+AGG 0.302384 1.1:-63295882 None:intergenic
AGCCGCTTCAACTGCTTCCT+TGG 0.345478 1.1:-63295769 None:intergenic
ATTACAATAAGTTAGGCTTT+CGG 0.353200 1.1:+63295814 MS.gene005399:CDS
TTGAACCTTTCCTGGCTCTC+TGG 0.363875 1.1:-63293781 None:intergenic
GTCACAAAGGCCAGAGAGCC+AGG 0.365783 1.1:+63293771 MS.gene005399:CDS
ACACAGGATGCAGGGGTACT+TGG 0.366534 1.1:+63295054 MS.gene005399:intron
GCCAGGAAAGGTTCAAAGTT+GGG 0.370354 1.1:+63293788 MS.gene005399:CDS
ATACATACCTCTAGATGAAT+TGG 0.374426 1.1:-63295135 None:intergenic
AAGGTGTATTACAATAAGTT+AGG 0.375769 1.1:+63295807 MS.gene005399:CDS
GGAGCTGCAAGGGATGAAAT+TGG 0.382447 1.1:-63293574 None:intergenic
TGAAAATCTGGGTGAGCATT+GGG 0.394785 1.1:-63293629 None:intergenic
CCTGAAAAGTGAGGCTAAAT+GGG 0.402843 1.1:-63293868 None:intergenic
ACAAGGAAAGGTCGTGGTAT+TGG 0.409504 1.1:+63293719 MS.gene005399:CDS
TCCTACGGCGAAGAGGGGTT+TGG 0.422318 1.1:-63293823 None:intergenic
GGGTTCTTGAAACCACGTTT+AGG 0.422852 1.1:-63293848 None:intergenic
TTGAAAATCTGGGTGAGCAT+TGG 0.433333 1.1:-63293630 None:intergenic
ATTGCAACATTTATCAATGC+TGG 0.438724 1.1:+63294904 MS.gene005399:CDS
AGGTAACAAGGGTCACTGTC+AGG 0.442200 1.1:+63295745 MS.gene005399:intron
ATTATCTCTCAGATCATTCA+AGG 0.446007 1.1:-63293678 None:intergenic
AAAGGTCGTGGTATTGGATC+TGG 0.457769 1.1:+63293725 MS.gene005399:CDS
TAAAGATGGAGTAAGATTGA+TGG 0.459426 1.1:+63295086 MS.gene005399:CDS
TCTGGAAAGGGAAAGACTGC+TGG 0.460358 1.1:+63293743 MS.gene005399:CDS
ACTCACAACAAATCCGTTCT+CGG 0.463672 1.1:+63293539 MS.gene005399:CDS
GGAATTGGCTTCTTCGGAGC+AGG 0.465181 1.1:-63295942 None:intergenic
CTCTAAAACCTTGAAAATCT+GGG 0.474320 1.1:-63293640 None:intergenic
AAAGGGAAAGACTGCTGGGA+GGG 0.483006 1.1:+63293748 MS.gene005399:CDS
CCTTTCTTCTCAAACCACTC+AGG 0.485963 1.1:-63295849 None:intergenic
AAAGATGGAGTAAGATTGAT+GGG 0.489075 1.1:+63295087 MS.gene005399:CDS
AGCCAGGAAAGGTTCAAAGT+TGG 0.492590 1.1:+63293787 MS.gene005399:CDS
CTTGGGAAGCAAATTAAAGA+TGG 0.493440 1.1:+63295072 MS.gene005399:CDS
ATTTAACTGAGCTGACCTAC+TGG 0.497431 1.1:-63295993 None:intergenic
AAGGATAAAGTTGATAGCAT+TGG 0.500169 1.1:+63295912 MS.gene005399:CDS
TATTACCATACACAGGATGC+AGG 0.500174 1.1:+63295045 MS.gene005399:intron
GAAGAAAGATGCTAAGAAGA+AGG 0.501124 1.1:+63293480 None:intergenic
CACAGGATGCAGGGGTACTT+GGG 0.501363 1.1:+63295055 MS.gene005399:intron
TAGACCTCCTCCAAAACAAA+AGG 0.503344 1.1:+63295893 MS.gene005399:CDS
GGCTGGTGGGTCCGTCAGAA+AGG 0.508668 1.1:+63295788 MS.gene005399:CDS
TAGGCATCCTACGGCGAAGA+GGG 0.511475 1.1:-63293829 None:intergenic
CTGGAAAGGGAAAGACTGCT+GGG 0.513550 1.1:+63293744 MS.gene005399:CDS
AAGGAAGCAGTTGAAGCGGC+TGG 0.517828 1.1:+63295771 MS.gene005399:CDS
CCTGAGTGGTTTGAGAAGAA+AGG 0.522455 1.1:+63295849 MS.gene005399:CDS
CCTCTAGATGAATTGGCCAT+TGG 0.524840 1.1:-63295128 None:intergenic
CGTGGTATTGGATCTGGAAA+GGG 0.539393 1.1:+63293731 MS.gene005399:CDS
AGGGATGAAATTGGATAGAC+GGG 0.542610 1.1:-63293565 None:intergenic
GGTTCAAAGTTGGGGTTCGA+AGG 0.550775 1.1:+63293797 MS.gene005399:CDS
AAGGGAAAGACTGCTGGGAG+GGG 0.550921 1.1:+63293749 MS.gene005399:CDS
GCTGACCTACTGGAAGATCC+TGG 0.552817 1.1:-63295983 None:intergenic
AAGGGATGAAATTGGATAGA+CGG 0.557057 1.1:-63293566 None:intergenic
GATAGACGGGTATCCGAGAA+CGG 0.557179 1.1:-63293552 None:intergenic
CCAGGAAAGGTTCAAAGTTG+GGG 0.558197 1.1:+63293789 MS.gene005399:CDS
TCGTGGTATTGGATCTGGAA+AGG 0.561440 1.1:+63293730 MS.gene005399:CDS
AAAGAGTGAGGGAGCTGCAA+GGG 0.562238 1.1:-63293584 None:intergenic
AAGGGTCACTGTCAGGGCCA+AGG 0.567491 1.1:+63295752 MS.gene005399:CDS
GGAGTAAGATTGATGGGGCG+TGG 0.570786 1.1:+63295093 MS.gene005399:CDS
GATCACAATGAAAACACTCA+AGG 0.573417 1.1:+63294948 MS.gene005399:CDS
GAAGCAGTTGAAGCGGCTGG+TGG 0.577528 1.1:+63295774 MS.gene005399:CDS
GGCCAAGGAAGCAGTTGAAG+CGG 0.578446 1.1:+63295767 MS.gene005399:CDS
AAGCAGTTGAAGCGGCTGGT+GGG 0.580431 1.1:+63295775 MS.gene005399:CDS
ATTGTAATACACCTTTCTGA+CGG 0.584521 1.1:-63295799 None:intergenic
GGTAACAAGGGTCACTGTCA+GGG 0.585974 1.1:+63295746 MS.gene005399:intron
CGAAAACAGAAGACAAGGAA+AGG 0.589508 1.1:+63293707 MS.gene005399:CDS
TATTGAGTTTACAGGTAACA+AGG 0.590526 1.1:+63295733 MS.gene005399:intron
TCAAAGTTGGGGTTCGAAGG+AGG 0.591062 1.1:+63293800 MS.gene005399:CDS
ATTACCATACACAGGATGCA+GGG 0.600741 1.1:+63295046 MS.gene005399:intron
AAAGGCCAGAGAGCCAGGAA+AGG 0.600747 1.1:+63293776 MS.gene005399:CDS
CAGAAGACAAGGAAAGGTCG+TGG 0.605789 1.1:+63293713 MS.gene005399:CDS
AGGCATCCTACGGCGAAGAG+GGG 0.607244 1.1:-63293828 None:intergenic
CCAATGGCCAATTCATCTAG+AGG 0.608787 1.1:+63295128 MS.gene005399:CDS
GCACGAACGTTGAAAGAGTG+AGG 0.615232 1.1:-63293596 None:intergenic
TTAGGCATCCTACGGCGAAG+AGG 0.618508 1.1:-63293830 None:intergenic
GAAAGAGTGAGGGAGCTGCA+AGG 0.621872 1.1:-63293585 None:intergenic
CGCCGTAGGATGCCTAAACG+TGG 0.624469 1.1:+63293836 MS.gene005399:CDS
AACCACGTTTAGGCATCCTA+CGG 0.626355 1.1:-63293838 None:intergenic
GTGGTTCTGAGAAGATCCAA+TGG 0.632368 1.1:+63295112 MS.gene005399:CDS
GAAAGGGAAAGACTGCTGGG+AGG 0.641614 1.1:+63293747 MS.gene005399:CDS
TTCCCCGAAAACAGAAGACA+AGG 0.642420 1.1:+63293702 MS.gene005399:CDS
GGGCACTGTTGAAGCCTGAG+TGG 0.644311 1.1:+63295835 MS.gene005399:CDS
GCCAAACCCCTCTTCGCCGT+AGG 0.645837 1.1:+63293822 MS.gene005399:CDS
CACGAACGTTGAAAGAGTGA+GGG 0.652354 1.1:-63293595 None:intergenic
GGAAGCCAGGATCTTCCAGT+AGG 0.653803 1.1:+63295978 MS.gene005399:CDS
ATTGAGTTTACAGGTAACAA+GGG 0.656620 1.1:+63295734 MS.gene005399:intron
ACTGCTGGGAGGGGTCACAA+AGG 0.661736 1.1:+63293758 MS.gene005399:CDS
AGAAGTATACCTGAAAAGTG+AGG 0.669464 1.1:-63293877 None:intergenic
ATAAAGTTGATAGCATTGGA+CGG 0.672998 1.1:+63295916 MS.gene005399:CDS
TTACCATACACAGGATGCAG+GGG 0.679526 1.1:+63295047 MS.gene005399:intron
GTGAGCATTGGGATTAAATG+TGG 0.701109 1.1:-63293618 None:intergenic
AAGATGGAGTAAGATTGATG+GGG 0.737220 1.1:+63295088 MS.gene005399:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAATATTCAAAACTAT+AGG - chr1.1:63294056-63294075 None:intergenic 10.0%
!! AACAAAAAAGATATATAAAC+AGG - chr1.1:63293904-63293923 None:intergenic 15.0%
!! GAGATAAATAAATGTAAAAT+TGG + chr1.1:63295627-63295646 MS.gene005399:intron 15.0%
!!! AAAGTATTTCTAAAGATTTA+AGG - chr1.1:63295220-63295239 None:intergenic 15.0%
!!! AAGATAAGATTTTTATTTCA+AGG + chr1.1:63294323-63294342 MS.gene005399:intron 15.0%
!!! ATTATAATTCTGAGATTTAT+GGG + chr1.1:63294068-63294087 MS.gene005399:intron 15.0%
!!! TATTATAATTCTGAGATTTA+TGG + chr1.1:63294067-63294086 MS.gene005399:intron 15.0%
!! AAATCTTCTGTAAACATATA+TGG + chr1.1:63294352-63294371 MS.gene005399:intron 20.0%
!! ATAATAAACAAAAGAATGAG+AGG - chr1.1:63295025-63295044 None:intergenic 20.0%
!! TAATAAACAAAAGAATGAGA+GGG - chr1.1:63295024-63295043 None:intergenic 20.0%
!! TGAACTCTAATAATATACTA+AGG - chr1.1:63294013-63294032 None:intergenic 20.0%
!! TTATAACATATTGAGTTTAC+AGG + chr1.1:63295725-63295744 MS.gene005399:intron 20.0%
!! TTATTATTATTACCATACAC+AGG + chr1.1:63295038-63295057 MS.gene005399:intron 20.0%
!!! AAACAAATTCATTTTAAGTG+AGG - chr1.1:63294989-63295008 None:intergenic 20.0%
!!! ACTTTTTTGAACTTATTTTG+AGG + chr1.1:63295243-63295262 MS.gene005399:intron 20.0%
!!! ATCCTTTTTAATAATATTCC+TGG - chr1.1:63294219-63294238 None:intergenic 20.0%
!!! TTTATTGTATTGACATTTGA+CGG + chr1.1:63294500-63294519 MS.gene005399:intron 20.0%
!!! TTTTCAATTCTAAACACTTA+AGG + chr1.1:63295655-63295674 MS.gene005399:intron 20.0%
! AAACTTTATAGGCATATAAG+AGG + chr1.1:63295698-63295717 MS.gene005399:intron 25.0%
! AACTTTATAGGCATATAAGA+GGG + chr1.1:63295699-63295718 MS.gene005399:intron 25.0%
! AAGGATAAAGAACAAAATGT+TGG + chr1.1:63295430-63295449 MS.gene005399:intron 25.0%
! AAGGTGTATTACAATAAGTT+AGG + chr1.1:63295807-63295826 MS.gene005399:CDS 25.0%
! AATGTCTAGATAAGTTTATG+TGG + chr1.1:63294716-63294735 MS.gene005399:intron 25.0%
! AATTGACATGTAGAAATAAG+AGG - chr1.1:63294414-63294433 None:intergenic 25.0%
! ACTGATAGATTGAGAAATAA+AGG - chr1.1:63294815-63294834 None:intergenic 25.0%
! ATTACAATAAGTTAGGCTTT+CGG + chr1.1:63295814-63295833 MS.gene005399:CDS 25.0%
! CTAAAGATTTAAGGATCAAA+AGG - chr1.1:63295211-63295230 None:intergenic 25.0%
! CTCCAGGAATATTATTAAAA+AGG + chr1.1:63294214-63294233 MS.gene005399:intron 25.0%
! CTGATAGATTGAGAAATAAA+GGG - chr1.1:63294814-63294833 None:intergenic 25.0%
! GAGTTCATTAGTTATAATTG+TGG + chr1.1:63294026-63294045 MS.gene005399:intron 25.0%
! TAAAATTTCTGTAGTGTCTA+AGG + chr1.1:63295411-63295430 MS.gene005399:intron 25.0%
! TAATAATATTCCTGGAGAAA+TGG - chr1.1:63294211-63294230 None:intergenic 25.0%
! TATACAGTCACAAACTTTAT+AGG + chr1.1:63295687-63295706 MS.gene005399:intron 25.0%
! TGATAGATTGAGAAATAAAG+GGG - chr1.1:63294813-63294832 None:intergenic 25.0%
!!! CTAAGTAATAAGTGTTTTTG+AGG + chr1.1:63295173-63295192 MS.gene005399:intron 25.0%
!!! GTATGTATACTTTGATTTGA+AGG + chr1.1:63295150-63295169 MS.gene005399:intron 25.0%
!!! TCAACTTTATCCTTTTGTTT+TGG - chr1.1:63295906-63295925 None:intergenic 25.0%
!!! TTTTTAGTGCATATCATACT+TGG - chr1.1:63294438-63294457 None:intergenic 25.0%
AAAGATGGAGTAAGATTGAT+GGG + chr1.1:63295087-63295106 MS.gene005399:CDS 30.0%
ACTTTATAGGCATATAAGAG+GGG + chr1.1:63295700-63295719 MS.gene005399:intron 30.0%
ATACATACCTCTAGATGAAT+TGG - chr1.1:63295138-63295157 None:intergenic 30.0%
ATGTTGCCAAAGTTAAAACA+TGG - chr1.1:63295381-63295400 None:intergenic 30.0%
ATTATCTCTCAGATCATTCA+AGG - chr1.1:63293681-63293700 None:intergenic 30.0%
ATTCTAAACACTTAAGGTGT+AGG + chr1.1:63295661-63295680 MS.gene005399:intron 30.0%
ATTCTTCTGTGTATTTAACC+GGG + chr1.1:63293935-63293954 MS.gene005399:intron 30.0%
ATTGAGTTTACAGGTAACAA+GGG + chr1.1:63295734-63295753 MS.gene005399:intron 30.0%
ATTGCAACATTTATCAATGC+TGG + chr1.1:63294904-63294923 MS.gene005399:CDS 30.0%
ATTGTAATACACCTTTCTGA+CGG - chr1.1:63295802-63295821 None:intergenic 30.0%
CACACACAGTTAAAATACAT+TGG - chr1.1:63294533-63294552 None:intergenic 30.0%
CCATGCTTAATGCAAATATT+AGG + chr1.1:63294271-63294290 MS.gene005399:intron 30.0%
CCTAATATTTGCATTAAGCA+TGG - chr1.1:63294274-63294293 None:intergenic 30.0%
CCTTCAACTAAAAATGGAAT+TGG - chr1.1:63295960-63295979 None:intergenic 30.0%
GATTCTTCTGTGTATTTAAC+CGG + chr1.1:63293934-63293953 MS.gene005399:intron 30.0%
TAAAGATGGAGTAAGATTGA+TGG + chr1.1:63295086-63295105 MS.gene005399:CDS 30.0%
TATTGAGTTTACAGGTAACA+AGG + chr1.1:63295733-63295752 MS.gene005399:intron 30.0%
TCAAGCAGAATAGTCTTTAT+TGG - chr1.1:63294167-63294186 None:intergenic 30.0%
TTACAATAAGTTAGGCTTTC+GGG + chr1.1:63295815-63295834 MS.gene005399:CDS 30.0%
! AAAAATGGAATTGGCTTCTT+CGG - chr1.1:63295951-63295970 None:intergenic 30.0%
! AATGGTTTTCTTTCTTGGAA+TGG - chr1.1:63294193-63294212 None:intergenic 30.0%
! AATTCTGAAAAAGTCTCAGA+TGG - chr1.1:63294123-63294142 None:intergenic 30.0%
! TTTAACTTTGGCAACATGAA+TGG + chr1.1:63295384-63295403 MS.gene005399:intron 30.0%
! TTTCTTCTTTTACAGCCAAT+AGG + chr1.1:63294871-63294890 MS.gene005399:intron 30.0%
!! AAGAACAAAATGTTGGCTTT+AGG + chr1.1:63295437-63295456 MS.gene005399:intron 30.0%
!! AAGGATAAAGTTGATAGCAT+TGG + chr1.1:63295912-63295931 MS.gene005399:CDS 30.0%
!! ATAAAGTTGATAGCATTGGA+CGG + chr1.1:63295916-63295935 MS.gene005399:CDS 30.0%
!! ATAATTCTGAGATTTATGGG+TGG + chr1.1:63294071-63294090 MS.gene005399:intron 30.0%
!! ATTCATTTTAAGTGAGGAAG+CGG - chr1.1:63294983-63295002 None:intergenic 30.0%
!! CTCTAAAACCTTGAAAATCT+GGG - chr1.1:63293643-63293662 None:intergenic 30.0%
!!! ACTTTATCCTTTTGTTTTGG+AGG - chr1.1:63295903-63295922 None:intergenic 30.0%
!!! CCAATTCCATTTTTAGTTGA+AGG + chr1.1:63295957-63295976 MS.gene005399:CDS 30.0%
!!! TTTTGAGCTTATTTTGATGC+TGG + chr1.1:63295294-63295313 MS.gene005399:intron 30.0%
AAGATGGAGTAAGATTGATG+GGG + chr1.1:63295088-63295107 MS.gene005399:CDS 35.0%
AAGGGATGAAATTGGATAGA+CGG - chr1.1:63293569-63293588 None:intergenic 35.0%
AGAAAGAAAACCATTTCTCC+AGG + chr1.1:63294198-63294217 MS.gene005399:intron 35.0%
AGATTGAGAAATAAAGGGGA+AGG - chr1.1:63294809-63294828 None:intergenic 35.0%
ATGGTTTACACTTGCCAAAA+GGG - chr1.1:63295343-63295362 None:intergenic 35.0%
CAAGTGTAAACCATCAAAAG+TGG + chr1.1:63295349-63295368 MS.gene005399:intron 35.0%
CTTGACACTCATAAATCTGA+AGG - chr1.1:63294619-63294638 None:intergenic 35.0%
CTTGGGAAGCAAATTAAAGA+TGG + chr1.1:63295072-63295091 MS.gene005399:CDS 35.0%
GAGTGTCAAGTAACTTCTAA+GGG + chr1.1:63294629-63294648 MS.gene005399:intron 35.0%
GATCACAATGAAAACACTCA+AGG + chr1.1:63294948-63294967 MS.gene005399:CDS 35.0%
TGAGTGTCAAGTAACTTCTA+AGG + chr1.1:63294628-63294647 MS.gene005399:intron 35.0%
TGATAAGTTCAAAAACACCG+TGG - chr1.1:63295270-63295289 None:intergenic 35.0%
TGGCTTCCTTCAACTAAAAA+TGG - chr1.1:63295966-63295985 None:intergenic 35.0%
TGGTTACTTGTGAGCAATAA+TGG + chr1.1:63294091-63294110 MS.gene005399:intron 35.0%
TGTAAACATATATGGCTGAG+TGG + chr1.1:63294360-63294379 MS.gene005399:intron 35.0%
! AGAAGTATACCTGAAAAGTG+AGG - chr1.1:63293880-63293899 None:intergenic 35.0%
! CTTTCCTTGTCTTCTGTTTT+CGG - chr1.1:63293709-63293728 None:intergenic 35.0%
! CTTTTACAGCCAATAGGTTT+GGG + chr1.1:63294877-63294896 MS.gene005399:intron 35.0%
! GCAATTTTTCCCAAACCTAT+TGG - chr1.1:63294889-63294908 None:intergenic 35.0%
! GGAGAAATGGTTTTCTTTCT+TGG - chr1.1:63294198-63294217 None:intergenic 35.0%
! TCTTTTACAGCCAATAGGTT+TGG + chr1.1:63294876-63294895 MS.gene005399:intron 35.0%
! TTTCCTTGTCTTCTGTTTTC+GGG - chr1.1:63293708-63293727 None:intergenic 35.0%
!! ATAAGTGTTTTTGAGGACCA+CGG + chr1.1:63295180-63295199 MS.gene005399:intron 35.0%
!! GCTCTAAAACCTTGAAAATC+TGG - chr1.1:63293644-63293663 None:intergenic 35.0%
!! TCGATTTTGCTATGAAGAGT+TGG + chr1.1:63295470-63295489 MS.gene005399:intron 35.0%
!!! ATTTTTAGTTGAAGGAAGCC+AGG + chr1.1:63295965-63295984 MS.gene005399:CDS 35.0%
!!! TGAACTTATTTTGAGGACCA+CGG + chr1.1:63295250-63295269 MS.gene005399:intron 35.0%
!!! TGACAGCCATGTTTTAACTT+TGG + chr1.1:63295372-63295391 MS.gene005399:intron 35.0%
!!! TTATCCTTTTGTTTTGGAGG+AGG - chr1.1:63295900-63295919 None:intergenic 35.0%
!!! TTTTAGAGCTTACAGTCTTC+TGG + chr1.1:63293655-63293674 MS.gene005399:CDS 35.0%
AAAGATACGAGCTTGAACCA+CGG - chr1.1:63294766-63294785 None:intergenic 40.0%
ACCTGAAAAGTGAGGCTAAA+TGG - chr1.1:63293872-63293891 None:intergenic 40.0%
ACTCACAACAAATCCGTTCT+CGG + chr1.1:63293539-63293558 MS.gene005399:CDS 40.0%
AGCAAACATTAGGTAGCCAA+TGG - chr1.1:63293968-63293987 None:intergenic 40.0%
AGGGATGAAATTGGATAGAC+GGG - chr1.1:63293568-63293587 None:intergenic 40.0%
ATTACCATACACAGGATGCA+GGG + chr1.1:63295046-63295065 MS.gene005399:intron 40.0%
CCTGAAAAGTGAGGCTAAAT+GGG - chr1.1:63293871-63293890 None:intergenic 40.0%
CGAAAACAGAAGACAAGGAA+AGG + chr1.1:63293707-63293726 MS.gene005399:CDS 40.0%
GATGGTTTACACTTGCCAAA+AGG - chr1.1:63295344-63295363 None:intergenic 40.0%
GCCAAAGTGAACTACACATT+TGG - chr1.1:63294585-63294604 None:intergenic 40.0%
GCCAAATGTGTAGTTCACTT+TGG + chr1.1:63294581-63294600 MS.gene005399:intron 40.0%
TAGACCTCCTCCAAAACAAA+AGG + chr1.1:63295893-63295912 MS.gene005399:CDS 40.0%
TATTACCATACACAGGATGC+AGG + chr1.1:63295045-63295064 MS.gene005399:intron 40.0%
TGAAAATCTGGGTGAGCATT+GGG - chr1.1:63293632-63293651 None:intergenic 40.0%
TTCAGCTTCACAATGATGCT+CGG - chr1.1:63294248-63294267 None:intergenic 40.0%
TTCCTGATGCAGCAAACATT+AGG - chr1.1:63293978-63293997 None:intergenic 40.0%
TTGAAAATCTGGGTGAGCAT+TGG - chr1.1:63293633-63293652 None:intergenic 40.0%
! AATGCTCACCCAGATTTTCA+AGG + chr1.1:63293632-63293651 MS.gene005399:CDS 40.0%
! TACCTAATGTTTGCTGCATC+AGG + chr1.1:63293973-63293992 MS.gene005399:intron 40.0%
! TTCCTTGTCTTCTGTTTTCG+GGG - chr1.1:63293707-63293726 None:intergenic 40.0%
!! GTGAGCATTGGGATTAAATG+TGG - chr1.1:63293621-63293640 None:intergenic 40.0%
AAAGGGGAAGGATACCAAAC+AGG - chr1.1:63294797-63294816 None:intergenic 45.0%
AACCACGTTTAGGCATCCTA+CGG - chr1.1:63293841-63293860 None:intergenic 45.0%
AAGGGGAAGGATACCAAACA+GGG - chr1.1:63294796-63294815 None:intergenic 45.0%
ACAAGGAAAGGTCGTGGTAT+TGG + chr1.1:63293719-63293738 MS.gene005399:CDS 45.0%
AGCCAGGAAAGGTTCAAAGT+TGG + chr1.1:63293787-63293806 MS.gene005399:CDS 45.0%
ATCTTTGGACCTTCCCTGTT+TGG + chr1.1:63294780-63294799 MS.gene005399:intron 45.0%
CACGAACGTTGAAAGAGTGA+GGG - chr1.1:63293598-63293617 None:intergenic 45.0%
CCAATGGCCAATTCATCTAG+AGG + chr1.1:63295128-63295147 MS.gene005399:CDS 45.0%
CCAGGAAAGGTTCAAAGTTG+GGG + chr1.1:63293789-63293808 MS.gene005399:CDS 45.0%
CCTCTAGATGAATTGGCCAT+TGG - chr1.1:63295131-63295150 None:intergenic 45.0%
CCTGAGTGGTTTGAGAAGAA+AGG + chr1.1:63295849-63295868 MS.gene005399:CDS 45.0%
CCTTTCTTCTCAAACCACTC+AGG - chr1.1:63295852-63295871 None:intergenic 45.0%
GCCAGGAAAGGTTCAAAGTT+GGG + chr1.1:63293788-63293807 MS.gene005399:CDS 45.0%
GGGTTCTTGAAACCACGTTT+AGG - chr1.1:63293851-63293870 None:intergenic 45.0%
GTGGTTCAAGCTCGTATCTT+TGG + chr1.1:63294765-63294784 MS.gene005399:intron 45.0%
TAGTCATAAATCCCTAGCCG+TGG + chr1.1:63294746-63294765 MS.gene005399:intron 45.0%
TCAGCTTCACAATGATGCTC+GGG - chr1.1:63294247-63294266 None:intergenic 45.0%
TTAACCGGGAACTTGACCAT+TGG + chr1.1:63293949-63293968 MS.gene005399:intron 45.0%
TTACCATACACAGGATGCAG+GGG + chr1.1:63295047-63295066 MS.gene005399:intron 45.0%
TTCCCCGAAAACAGAAGACA+AGG + chr1.1:63293702-63293721 MS.gene005399:CDS 45.0%
! AAAGGTCGTGGTATTGGATC+TGG + chr1.1:63293725-63293744 MS.gene005399:CDS 45.0%
! CAAAAGGGAAAAGAGAGTGC+AGG - chr1.1:63295328-63295347 None:intergenic 45.0%
! CGTGGTATTGGATCTGGAAA+GGG + chr1.1:63293731-63293750 MS.gene005399:CDS 45.0%
! CTGCACTCTCTTTTCCCTTT+TGG + chr1.1:63295326-63295345 MS.gene005399:intron 45.0%
! TCGTGGTATTGGATCTGGAA+AGG + chr1.1:63293730-63293749 MS.gene005399:CDS 45.0%
!! ATGGCTGTCACCACTTTTGA+TGG - chr1.1:63295362-63295381 None:intergenic 45.0%
!! CCCATTTAGCCTCACTTTTC+AGG + chr1.1:63293868-63293887 MS.gene005399:CDS 45.0%
!! GTGGTTCTGAGAAGATCCAA+TGG + chr1.1:63295112-63295131 MS.gene005399:CDS 45.0%
AAAGAGTGAGGGAGCTGCAA+GGG - chr1.1:63293587-63293606 None:intergenic 50.0%
AAAGGGAAAGACTGCTGGGA+GGG + chr1.1:63293748-63293767 MS.gene005399:CDS 50.0%
AGGTAACAAGGGTCACTGTC+AGG + chr1.1:63295745-63295764 MS.gene005399:intron 50.0%
CAGAAGACAAGGAAAGGTCG+TGG + chr1.1:63293713-63293732 MS.gene005399:CDS 50.0%
CCCCAACTTTGAACCTTTCC+TGG - chr1.1:63293792-63293811 None:intergenic 50.0%
CTGGAAAGGGAAAGACTGCT+GGG + chr1.1:63293744-63293763 MS.gene005399:CDS 50.0%
GATAGACGGGTATCCGAGAA+CGG - chr1.1:63293555-63293574 None:intergenic 50.0%
GCACGAACGTTGAAAGAGTG+AGG - chr1.1:63293599-63293618 None:intergenic 50.0%
GGAAGGATACCAAACAGGGA+AGG - chr1.1:63294792-63294811 None:intergenic 50.0%
GGAGCTGCAAGGGATGAAAT+TGG - chr1.1:63293577-63293596 None:intergenic 50.0%
GGTAACAAGGGTCACTGTCA+GGG + chr1.1:63295746-63295765 MS.gene005399:intron 50.0%
GGTTCAAAGTTGGGGTTCGA+AGG + chr1.1:63293797-63293816 MS.gene005399:CDS 50.0%
GTAGCCAATGGTCAAGTTCC+CGG - chr1.1:63293956-63293975 None:intergenic 50.0%
TCAAAGTTGGGGTTCGAAGG+AGG + chr1.1:63293800-63293819 MS.gene005399:CDS 50.0%
TCTGGAAAGGGAAAGACTGC+TGG + chr1.1:63293743-63293762 MS.gene005399:CDS 50.0%
! TTGAACCTTTCCTGGCTCTC+TGG - chr1.1:63293784-63293803 None:intergenic 50.0%
!! AGGATCAAAAGGCATCACCG+TGG - chr1.1:63295200-63295219 None:intergenic 50.0%
AAAGGCCAGAGAGCCAGGAA+AGG + chr1.1:63293776-63293795 MS.gene005399:CDS 55.0%
AAGCAGTTGAAGCGGCTGGT+GGG + chr1.1:63295775-63295794 MS.gene005399:CDS 55.0%
AAGGAAGCAGTTGAAGCGGC+TGG + chr1.1:63295771-63295790 MS.gene005399:CDS 55.0%
AAGGGAAAGACTGCTGGGAG+GGG + chr1.1:63293749-63293768 MS.gene005399:CDS 55.0%
ACGAGCTTGAACCACGGCTA+GGG - chr1.1:63294760-63294779 None:intergenic 55.0%
AGCCGCTTCAACTGCTTCCT+TGG - chr1.1:63295772-63295791 None:intergenic 55.0%
GAAAGAGTGAGGGAGCTGCA+AGG - chr1.1:63293588-63293607 None:intergenic 55.0%
GAAAGGGAAAGACTGCTGGG+AGG + chr1.1:63293747-63293766 MS.gene005399:CDS 55.0%
GCTGACCTACTGGAAGATCC+TGG - chr1.1:63295986-63296005 None:intergenic 55.0%
GCTGAGTGGCTTGACTGTGT+TGG + chr1.1:63294374-63294393 MS.gene005399:intron 55.0%
GGAAGCCAGGATCTTCCAGT+AGG + chr1.1:63295978-63295997 MS.gene005399:CDS 55.0%
GGAGTAAGATTGATGGGGCG+TGG + chr1.1:63295093-63295112 MS.gene005399:CDS 55.0%
GGCCAAGGAAGCAGTTGAAG+CGG + chr1.1:63295767-63295786 MS.gene005399:CDS 55.0%
GTACCCCTGCATCCTGTGTA+TGG - chr1.1:63295053-63295072 None:intergenic 55.0%
TACGAGCTTGAACCACGGCT+AGG - chr1.1:63294761-63294780 None:intergenic 55.0%
TAGGCATCCTACGGCGAAGA+GGG - chr1.1:63293832-63293851 None:intergenic 55.0%
TTAGGCATCCTACGGCGAAG+AGG - chr1.1:63293833-63293852 None:intergenic 55.0%
! GGAATTGGCTTCTTCGGAGC+AGG - chr1.1:63295945-63295964 None:intergenic 55.0%
! GGAGGAGGTCTAGCTGCTTT+AGG - chr1.1:63295885-63295904 None:intergenic 55.0%
!! ACACAGGATGCAGGGGTACT+TGG + chr1.1:63295054-63295073 MS.gene005399:intron 55.0%
!! CACAGGATGCAGGGGTACTT+GGG + chr1.1:63295055-63295074 MS.gene005399:intron 55.0%
AAGGGTCACTGTCAGGGCCA+AGG + chr1.1:63295752-63295771 MS.gene005399:CDS 60.0%
ACTGCTGGGAGGGGTCACAA+AGG + chr1.1:63293758-63293777 MS.gene005399:CDS 60.0%
AGGCATCCTACGGCGAAGAG+GGG - chr1.1:63293831-63293850 None:intergenic 60.0%
CGCCGTAGGATGCCTAAACG+TGG + chr1.1:63293836-63293855 MS.gene005399:CDS 60.0%
GAAGCAGTTGAAGCGGCTGG+TGG + chr1.1:63295774-63295793 MS.gene005399:CDS 60.0%
GTCACAAAGGCCAGAGAGCC+AGG + chr1.1:63293771-63293790 MS.gene005399:CDS 60.0%
TCCTACGGCGAAGAGGGGTT+TGG - chr1.1:63293826-63293845 None:intergenic 60.0%
! GGGCACTGTTGAAGCCTGAG+TGG + chr1.1:63295835-63295854 MS.gene005399:CDS 60.0%
GCCAAACCCCTCTTCGCCGT+AGG + chr1.1:63293822-63293841 MS.gene005399:CDS 65.0%
! GGCTGGTGGGTCCGTCAGAA+AGG + chr1.1:63295788-63295807 MS.gene005399:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 63293488 63296013 63293488 ID=MS.gene005399
chr1.1 mRNA 63293488 63296013 63293488 ID=MS.gene005399.t1;Parent=MS.gene005399
chr1.1 exon 63293488 63293889 63293488 ID=MS.gene005399.t1.exon1;Parent=MS.gene005399.t1
chr1.1 CDS 63293488 63293889 63293488 ID=cds.MS.gene005399.t1;Parent=MS.gene005399.t1
chr1.1 exon 63294886 63294969 63294886 ID=MS.gene005399.t1.exon2;Parent=MS.gene005399.t1
chr1.1 CDS 63294886 63294969 63294886 ID=cds.MS.gene005399.t1;Parent=MS.gene005399.t1
chr1.1 exon 63295060 63295149 63295060 ID=MS.gene005399.t1.exon3;Parent=MS.gene005399.t1
chr1.1 CDS 63295060 63295149 63295060 ID=cds.MS.gene005399.t1;Parent=MS.gene005399.t1
chr1.1 exon 63295747 63296013 63295747 ID=MS.gene005399.t1.exon4;Parent=MS.gene005399.t1
chr1.1 CDS 63295747 63296013 63295747 ID=cds.MS.gene005399.t1;Parent=MS.gene005399.t1
Gene Sequence

>MS.gene005399

ATGCTAAGAAGAAGGCTTTCTGTTCTCTCGACTTCAATCATCAGAAGTTCGACTCACAACAAATCCGTTCTCGGATACCCGTCTATCCAATTTCATCCCTTGCAGCTCCCTCACTCTTTCAACGTTCGTGCCACATTTAATCCCAATGCTCACCCAGATTTTCAAGGTTTTAGAGCTTACAGTCTTCTGGCCTTGAATGATCTGAGAGATAATGTTCCCCGAAAACAGAAGACAAGGAAAGGTCGTGGTATTGGATCTGGAAAGGGAAAGACTGCTGGGAGGGGTCACAAAGGCCAGAGAGCCAGGAAAGGTTCAAAGTTGGGGTTCGAAGGAGGCCAAACCCCTCTTCGCCGTAGGATGCCTAAACGTGGTTTCAAGAACCCATTTAGCCTCACTTTTCAGGTATACTTCTTCCTGTTTATATATCTTTTTTGTTTTTGATTAATGATTCTTCTGTGTATTTAACCGGGAACTTGACCATTGGCTACCTAATGTTTGCTGCATCAGGAAAATGAAATTACGCCTTAGTATATTATTAGAGTTCATTAGTTATAATTGTGGAACTCCTATAGTTTTGAATATTATAATTCTGAGATTTATGGGTGGTTACTTGTGAGCAATAATGGAGTTCTCCATCTGAGACTTTTTCAGAATTTCTTTTGCATACTTATTAAAACCAATAAAGACTATTCTGCTTGACTTCCATTCCAAGAAAGAAAACCATTTCTCCAGGAATATTATTAAAAAGGATGCATACCCGAGCATCATTGTGAAGCTGAAGTGCCATGCTTAATGCAAATATTAGGTTTTAATTTTATTACAAGACATCATATGCAAGATAAGATTTTTATTTCAAGGACTCTGAAATCTTCTGTAAACATATATGGCTGAGTGGCTTGACTGTGTTGGTTTTGATTGATAAACCTCTTATTTCTACATGTCAATTGCCAAGTATGATATGCACTAAAAAGTAATGTGCATGTCGACAACAAATATATTTCAAAGTTTATACTTTATTGTATTGACATTTGACGGATATTTACCAATGTATTTTAACTGTGTGTGTGTGATTTTTGATATAGAATGACATAATGCCAAATGTGTAGTTCACTTTGGCTGATATTTGAGCCTTCAGATTTATGAGTGTCAAGTAACTTCTAAGGGACTATTCTAGTTGTATTAAATAATTTTGTGTGCATAATTTAATTCTTTAATATTTTTTTATTTTAATGTCTAGATAAGTTTATGTGGAGTAGAGTAGTCATAAATCCCTAGCCGTGGTTCAAGCTCGTATCTTTGGACCTTCCCTGTTTGGTATCCTTCCCCTTTATTTCTCAATCTATCAGTCATATTATCTACTGCTGTATTCAGAAGTTTGTTTTATTTCTTCTTTTACAGCCAATAGGTTTGGGAAAAATTGCAACATTTATCAATGCTGGCAAGATAGATTCTTCTGAATTGATCACAATGAAAACACTCAAGGTTTTTGTGACCGCTTCCTCACTTAAAATGAATTTGTTTGCTTCATGTGCTCCCTCTCATTCTTTTGTTTATTATTATTACCATACACAGGATGCAGGGGTACTTGGGAAGCAAATTAAAGATGGAGTAAGATTGATGGGGCGTGGTTCTGAGAAGATCCAATGGCCAATTCATCTAGAGGTATGTATACTTTGATTTGAAGGCTAAGTAATAAGTGTTTTTGAGGACCACGGTGATGCCTTTTGATCCTTAAATCTTTAGAAATACTTTTTAACTTTTTTGAACTTATTTTGAGGACCACGGTGTTTTTGAACTTATCATTAATTTTGAGCTTATTTTGATGCTGGCTGCAAAGCCTGCACTCTCTTTTCCCTTTTGGCAAGTGTAAACCATCAAAAGTGGTGACAGCCATGTTTTAACTTTGGCAACATGAATGGTATATAAAATTTCTGTAGTGTCTAAGGATAAAGAACAAAATGTTGGCTTTAGGTTTCGTAATTTCGATTTTGCTATGAAGAGTTGGAATAGCTCTTATATCTTCAAAGTTTACTTGCTAATTTTCTCAAACTTAGACATTTTCTGTTTTGTATTTCAAATAAAGAATTAAACTCATATAGTATGAGATTTTGATTACATGTTATGCTTCTGTTTGTCAATGAGATAAATAAATGTAAAATTGGTACTTTTTTCAATTCTAAACACTTAAGGTGTAGGTTTTATACAGTCACAAACTTTATAGGCATATAAGAGGGGTGTTATAACATATTGAGTTTACAGGTAACAAGGGTCACTGTCAGGGCCAAGGAAGCAGTTGAAGCGGCTGGTGGGTCCGTCAGAAAGGTGTATTACAATAAGTTAGGCTTTCGGGCACTGTTGAAGCCTGAGTGGTTTGAGAAGAAAGGAAGATTGTTGCCTAAAGCAGCTAGACCTCCTCCAAAACAAAAGGATAAAGTTGATAGCATTGGACGGCTGCCTGCTCCGAAGAAGCCAATTCCATTTTTAGTTGAAGGAAGCCAGGATCTTCCAGTAGGTCAGCTCAGTTAA
Protein sequence

>MS.gene005399.t1

MLRRRLSVLSTSIIRSSTHNKSVLGYPSIQFHPLQLPHSFNVRATFNPNAHPDFQGFRAYSLLALNDLRDNVPRKQKTRKGRGIGSGKGKTAGRGHKGQRARKGSKLGFEGGQTPLRRRMPKRGFKNPFSLTFQPIGLGKIATFINAGKIDSSELITMKTLKDAGVLGKQIKDGVRLMGRGSEKIQWPIHLEVTRVTVRAKEAVEAAGGSVRKVYYNKLGFRALLKPEWFEKKGRLLPKAARPPPKQKDKVDSIGRLPAPKKPIPFLVEGSQDLPVGQLS