AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005865

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005865.t1 MTR_1g093060 98.299 294 5 0 1 294 54 347 0.0 597
MS.gene005865.t1 MTR_6g006970 92.857 294 21 0 1 294 1 294 0.0 572
MS.gene005865.t1 MTR_2g090370 93.269 104 7 0 189 292 1 104 2.40e-68 213
MS.gene005865.t1 MTR_3g118210 41.063 207 116 6 49 251 58 262 6.36e-43 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005865.t1 AT3G17465 66.975 324 77 4 1 294 1 324 1.77e-147 417
MS.gene005865.t1 AT2G43030 38.835 206 122 4 49 251 57 261 4.59e-39 138

Find 84 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGGATGTGGATCCATTTA+TGG 0.158517 1.1:-67504025 MS.gene005865:CDS
ACACCAGTTCTTTGTGAATT+TGG 0.165812 1.1:+67506757 None:intergenic
TGACTGCTCATTGGGATAAA+TGG 0.166624 1.1:-67506714 MS.gene005865:CDS
CTCACTGTTCAGGTCTTTAA+AGG 0.228704 1.1:-67504824 MS.gene005865:intron
CTTTAATCAGTAACCATAAA+TGG 0.233502 1.1:+67504012 None:intergenic
GAATTCCAATTTCTATCCTT+TGG 0.239774 1.1:-67506684 MS.gene005865:CDS
AAACCTTTGGTTGCTGATCT+TGG 0.318983 1.1:-67504045 MS.gene005865:CDS
TCTACCCAAAGGATAGAAAT+TGG 0.334271 1.1:+67506679 None:intergenic
AACCTTTGGTTGCTGATCTT+GGG 0.341776 1.1:-67504044 MS.gene005865:CDS
ACCCACATCAAATTTCTTGC+TGG 0.343726 1.1:+67504731 None:intergenic
GTTCAGATGTGGAGACTATA+AGG 0.352256 1.1:-67506810 MS.gene005865:CDS
CAGGGTGTAATGAAGAAGTA+TGG 0.352968 1.1:-67505052 MS.gene005865:CDS
CATACTGCCCAGGAACAAAA+TGG 0.372996 1.1:+67505875 None:intergenic
GCCCTAAAATGACCCACTTC+AGG 0.379527 1.1:+67505985 None:intergenic
TCATCTGAATCTTCATCTTC+CGG 0.389387 1.1:+67504072 None:intergenic
TTCAGATGATATGAAACCTT+TGG 0.395479 1.1:-67504058 MS.gene005865:CDS
AATTCCAATTTCTATCCTTT+GGG 0.400773 1.1:-67506683 MS.gene005865:CDS
ATGAAGAAGTATGGATTTCA+TGG 0.403214 1.1:-67505043 MS.gene005865:CDS
GACTGCTCATTGGGATAAAT+GGG 0.404613 1.1:-67506713 MS.gene005865:CDS
GGTTGCTGATCTTGGGGATG+TGG 0.407346 1.1:-67504037 MS.gene005865:CDS
GAACAATAGCCTTCCTTCTC+AGG 0.410835 1.1:+67506628 None:intergenic
TAGAAAGATGCCAGGGAGAA+TGG 0.416986 1.1:-67504801 MS.gene005865:CDS
TGCTTCCCACCCATTCTCCC+TGG 0.421120 1.1:+67504791 None:intergenic
ACAAAGTTCCCTGTAGCTCC+TGG 0.431609 1.1:+67504162 None:intergenic
AGGAATACTTGGTCCTTGTA+AGG 0.431878 1.1:-67504969 MS.gene005865:intron
GATTGGTTGTGGACAGAAGA+AGG 0.441589 1.1:-67506025 MS.gene005865:CDS
ACACAGGTCCCAGGAGCTAC+AGG 0.448282 1.1:-67504171 MS.gene005865:intron
ACCTTTGGTTGCTGATCTTG+GGG 0.448994 1.1:-67504043 MS.gene005865:CDS
ATGTGGAATGACTGCTCATT+GGG 0.453159 1.1:-67506722 MS.gene005865:CDS
CATAGAACCATGGGTTCTAC+AGG 0.456921 1.1:-67504989 MS.gene005865:CDS
TCTTCCGGCACAAAGTAAGT+TGG 0.469195 1.1:+67504087 None:intergenic
AGATGACAACATTGTCTCTC+AGG 0.475183 1.1:-67506659 MS.gene005865:CDS
ACACCAAATTCACAAAGAAC+TGG 0.478282 1.1:-67506760 MS.gene005865:CDS
CTTCCGGCACAAAGTAAGTT+GGG 0.483444 1.1:+67504088 None:intergenic
AGAAAGATGCCAGGGAGAAT+GGG 0.487342 1.1:-67504800 MS.gene005865:CDS
AGAAATTTGATGTGGGTGAA+AGG 0.489286 1.1:-67504725 MS.gene005865:CDS
GGACTCCATCCAACCTTACA+AGG 0.491579 1.1:+67504956 None:intergenic
AATCGCGGCAGTGGTGCCAC+GGG 0.495730 1.1:+67506838 None:intergenic
ACAGGAATCACCAAAGGAAA+AGG 0.499760 1.1:-67505844 MS.gene005865:CDS
AATGTGGAATGACTGCTCAT+TGG 0.503569 1.1:-67506723 MS.gene005865:CDS
ACATTAAGTGATGTACCAAC+AGG 0.507292 1.1:+67505901 None:intergenic
TATGTGCTTTCGAATTCCCA+TGG 0.513084 1.1:+67505008 None:intergenic
TCTTGAAGCTTCCTCTTCAT+CGG 0.518555 1.1:+67505952 None:intergenic
GAATTTGGTGTCATCACACT+AGG 0.526237 1.1:+67506772 None:intergenic
CTCAAGGAGTTCCGATGAAG+AGG 0.527266 1.1:-67505963 MS.gene005865:CDS
TTTGATGTGGGTGAAAGGCC+AGG 0.528326 1.1:-67504720 MS.gene005865:intron
AAAGGAAGTATAGATGTGTC+AGG 0.528945 1.1:+67504111 None:intergenic
GGAAGGCTATTGTTCTCTCC+AGG 0.530451 1.1:-67506620 MS.gene005865:intron
ATGGGTTCTACAGGAATACT+TGG 0.533632 1.1:-67504980 MS.gene005865:CDS
AAATCGCGGCAGTGGTGCCA+CGG 0.542479 1.1:+67506837 None:intergenic
GAGGATGCACTTCTTCCTGT+TGG 0.552055 1.1:-67505916 MS.gene005865:CDS
TTTCCCAACTTACTTTGTGC+CGG 0.552953 1.1:-67504091 MS.gene005865:CDS
CAAAGTTCCCTGTAGCTCCT+GGG 0.553971 1.1:+67504163 None:intergenic
CATGGAGGTTCAGCATCCCA+TGG 0.557351 1.1:-67505025 MS.gene005865:CDS
ACACTTGACAAAGCCTGAAG+TGG 0.560843 1.1:-67505998 MS.gene005865:CDS
TTTAAAGGTAGAAAGATGCC+AGG 0.564006 1.1:-67504809 MS.gene005865:CDS
TCAGGTCAAAACTCCTGAGA+AGG 0.567631 1.1:-67506641 MS.gene005865:CDS
ATGTGCTTTCGAATTCCCAT+GGG 0.571228 1.1:+67505009 None:intergenic
TCAAGAGTTTCGAGTGACAG+AGG 0.581725 1.1:-67505935 MS.gene005865:CDS
GTGTGCACAACACAGGTCCC+AGG 0.582644 1.1:-67504180 MS.gene005865:intron
GGGCAGTATGTTGACATCAC+AGG 0.583754 1.1:-67505862 MS.gene005865:CDS
ATGGAGGTTCAGCATCCCAT+GGG 0.591854 1.1:-67505024 MS.gene005865:CDS
AAGATGCCAGGGAGAATGGG+TGG 0.599236 1.1:-67504797 MS.gene005865:CDS
AAGTATTCCTGTAGAACCCA+TGG 0.604216 1.1:+67504982 None:intergenic
TCGAAAGCACATAGAACCAT+GGG 0.605881 1.1:-67504998 MS.gene005865:CDS
GGCACAAAGTAAGTTGGGAA+AGG 0.607369 1.1:+67504093 None:intergenic
GTGATGTCAACATACTGCCC+AGG 0.609175 1.1:+67505865 None:intergenic
ACCTCTTGAAAACGCCAGCA+TGG 0.609546 1.1:+67506885 None:intergenic
CACTTGACAAAGCCTGAAGT+GGG 0.609676 1.1:-67505997 MS.gene005865:CDS
GTCAAAACTCCTGAGAAGGA+AGG 0.621009 1.1:-67506637 MS.gene005865:CDS
ATCCAGCAAGAAATTTGATG+TGG 0.622717 1.1:-67504733 MS.gene005865:CDS
TTCGAAAGCACATAGAACCA+TGG 0.623227 1.1:-67504999 MS.gene005865:CDS
CACAGGTCCCAGGAGCTACA+GGG 0.625211 1.1:-67504170 MS.gene005865:intron
AGATGCCAGGGAGAATGGGT+GGG 0.644345 1.1:-67504796 MS.gene005865:CDS
GGTGTTATAGCTGTAAAATG+TGG 0.647527 1.1:-67506739 MS.gene005865:CDS
TCCCCAAGATCAGCAACCAA+AGG 0.647605 1.1:+67504042 None:intergenic
GACATCACAGGAATCACCAA+AGG 0.651363 1.1:-67505850 MS.gene005865:CDS
CAATGTTGTCATCTACCCAA+AGG 0.657641 1.1:+67506668 None:intergenic
TTAAAGGTAGAAAGATGCCA+GGG 0.664670 1.1:-67504808 MS.gene005865:CDS
TCCAGCAAGAAATTTGATGT+GGG 0.677547 1.1:-67504732 MS.gene005865:CDS
AAGAAGTATGGATTTCATGG+AGG 0.684184 1.1:-67505040 MS.gene005865:CDS
ATCGCGGCAGTGGTGCCACG+GGG 0.726950 1.1:+67506839 None:intergenic
TCGTCTTCGACAATTCCCCG+TGG 0.741512 1.1:-67506854 MS.gene005865:CDS
ACTGCTCATTGGGATAAATG+GGG 0.750912 1.1:-67506712 MS.gene005865:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTAAATTTGTATTTGT+AGG - chr1.1:67505288-67505307 MS.gene005865:intron 10.0%
!! AATGTTTATAATGAATAATG+CGG + chr1.1:67504429-67504448 None:intergenic 15.0%
!! CTACTAAATTAAATACAAAA+TGG + chr1.1:67506624-67506643 None:intergenic 15.0%
!! GACAGAAATTATTATTTATA+TGG + chr1.1:67505130-67505149 None:intergenic 15.0%
!! GTTTATAATGAGTTATTAAA+TGG - chr1.1:67504736-67504755 MS.gene005865:CDS 15.0%
!! TTGAACAAAACAATAAATAA+TGG + chr1.1:67505478-67505497 None:intergenic 15.0%
!! TTGATTACATTAGTTATATT+AGG - chr1.1:67505357-67505376 MS.gene005865:intron 15.0%
!! AAAGAACAGTAAAAAATGTA+TGG - chr1.1:67506128-67506147 MS.gene005865:intron 20.0%
!! AAGAACAGTAAAAAATGTAT+GGG - chr1.1:67506129-67506148 MS.gene005865:intron 20.0%
!! ACAAAAAAACAATCATCTTA+GGG + chr1.1:67505328-67505347 None:intergenic 20.0%
!! CAAATTCAATCATGTATTAT+AGG - chr1.1:67505452-67505471 MS.gene005865:intron 20.0%
!! GCAGAAAAATAAACAATAAA+TGG + chr1.1:67505692-67505711 None:intergenic 20.0%
!! TACAAAAAAACAATCATCTT+AGG + chr1.1:67505329-67505348 None:intergenic 20.0%
!! TATATATAGAAGACATATCT+AGG + chr1.1:67504793-67504812 None:intergenic 20.0%
!! TGTCAACTTATTATATCATT+AGG + chr1.1:67505434-67505453 None:intergenic 20.0%
!! TTATTATATCATTAGGATTC+AGG + chr1.1:67505427-67505446 None:intergenic 20.0%
!! TTCACATTAATTTATGAACT+TGG - chr1.1:67506223-67506242 MS.gene005865:intron 20.0%
!!! AAATTATATTTTCTGTTTCC+AGG - chr1.1:67505826-67505845 MS.gene005865:intron 20.0%
!!! AAGATGATTGTTTTTTTGTA+TGG - chr1.1:67505329-67505348 MS.gene005865:intron 20.0%
!!! AATTATATTTTCTGTTTCCA+GGG - chr1.1:67505827-67505846 MS.gene005865:intron 20.0%
!!! ACACGTTTTTTTTTTTTGAA+TGG - chr1.1:67505782-67505801 MS.gene005865:intron 20.0%
!!! AGATGATTGTTTTTTTGTAT+GGG - chr1.1:67505330-67505349 MS.gene005865:intron 20.0%
!!! TTTTGATGTACAAAATTTCT+TGG + chr1.1:67506559-67506578 None:intergenic 20.0%
! AACATGTAAAAACAATTACC+TGG + chr1.1:67506198-67506217 None:intergenic 25.0%
! AACTAAGAATACTAATCTGA+AGG + chr1.1:67505965-67505984 None:intergenic 25.0%
! AATTCCAATTTCTATCCTTT+GGG - chr1.1:67504214-67504233 MS.gene005865:intron 25.0%
! ACAGTAAAAAAAGCAGAATA+TGG - chr1.1:67504680-67504699 MS.gene005865:intron 25.0%
! ATTTACTTCTAGACAATCAT+TGG - chr1.1:67505519-67505538 MS.gene005865:intron 25.0%
! GATTGCATAAATACAAATTC+TGG - chr1.1:67504583-67504602 MS.gene005865:intron 25.0%
! TAGCTCAATTATTCATTAAC+TGG - chr1.1:67506641-67506660 MS.gene005865:CDS 25.0%
! TTGAATTTCACCAATCTAAT+CGG + chr1.1:67504715-67504734 None:intergenic 25.0%
!! ATATGAAAGTATGTAATACG+AGG + chr1.1:67505101-67505120 None:intergenic 25.0%
!! GGCAAAAATATTTGAAATCT+CGG - chr1.1:67506662-67506681 MS.gene005865:CDS 25.0%
!! TATGAAAGTATGTAATACGA+GGG + chr1.1:67505100-67505119 None:intergenic 25.0%
!!! ATGCTTTGTTTTAGTTAGAT+TGG - chr1.1:67504855-67504874 MS.gene005865:intron 25.0%
AAAAAAACGTGTAACTGACA+AGG + chr1.1:67505774-67505793 None:intergenic 30.0%
AATCCACATGAAACTCATTA+GGG + chr1.1:67504339-67504358 None:intergenic 30.0%
ACATCTGAACTAAAAAATCG+CGG + chr1.1:67504077-67504096 None:intergenic 30.0%
ACTTGATCCTTAACATAGTA+TGG - chr1.1:67504626-67504645 MS.gene005865:intron 30.0%
ATACACAAATCAAGTGATTG+AGG - chr1.1:67506278-67506297 MS.gene005865:intron 30.0%
ATTATCGCCATACTATGTTA+AGG + chr1.1:67504636-67504655 None:intergenic 30.0%
ATTTATGTTTCTCACTGTTC+AGG - chr1.1:67506063-67506082 MS.gene005865:intron 30.0%
CGAAAATTTGTGTAGTTGAT+TGG + chr1.1:67504465-67504484 None:intergenic 30.0%
CGTTTGTTTATTGACATGAT+TGG - chr1.1:67505246-67505265 MS.gene005865:intron 30.0%
GAATGCTTATTCTCTTTGTA+TGG - chr1.1:67506695-67506714 MS.gene005865:CDS 30.0%
GAATTCCAATTTCTATCCTT+TGG - chr1.1:67504213-67504232 MS.gene005865:intron 30.0%
GGATGAATCAATCATATTCA+TGG - chr1.1:67506328-67506347 MS.gene005865:intron 30.0%
TAATATCATCAAGAGAACGA+TGG - chr1.1:67506588-67506607 MS.gene005865:intron 30.0%
TAATCCACATGAAACTCATT+AGG + chr1.1:67504340-67504359 None:intergenic 30.0%
TACATGTTTATGCTTGTATG+TGG - chr1.1:67505173-67505192 MS.gene005865:intron 30.0%
TTCAGATGATATGAAACCTT+TGG - chr1.1:67506839-67506858 MS.gene005865:CDS 30.0%
TTGAAATCTCGGATTTATCA+CGG - chr1.1:67506673-67506692 MS.gene005865:CDS 30.0%
TTGTCATTCATGTATGATTG+CGG - chr1.1:67505724-67505743 MS.gene005865:intron 30.0%
TTGTGTAGTTGATTGGATTT+CGG + chr1.1:67504458-67504477 None:intergenic 30.0%
! ATGAAGAAGTATGGATTTCA+TGG - chr1.1:67505854-67505873 MS.gene005865:CDS 30.0%
!! AATCACTTGATTTGTGTATG+AGG + chr1.1:67506277-67506296 None:intergenic 30.0%
!! GGGTAAGTAAAGTTTTAACA+TGG + chr1.1:67506364-67506383 None:intergenic 30.0%
!!! TGTTTTAGTTAGATTGGTTG+TGG - chr1.1:67504861-67504880 MS.gene005865:intron 30.0%
!!! TTTTTTGAATGGCAGTTACA+CGG - chr1.1:67505793-67505812 MS.gene005865:intron 30.0%
ACACCAAATTCACAAAGAAC+TGG - chr1.1:67504137-67504156 MS.gene005865:CDS 35.0%
ACACCAGTTCTTTGTGAATT+TGG + chr1.1:67504143-67504162 None:intergenic 35.0%
ACATTAAGTGATGTACCAAC+AGG + chr1.1:67504999-67505018 None:intergenic 35.0%
AGAAATTTGATGTGGGTGAA+AGG - chr1.1:67506172-67506191 MS.gene005865:intron 35.0%
ATCCAGCAAGAAATTTGATG+TGG - chr1.1:67506164-67506183 MS.gene005865:intron 35.0%
ATTTATGAACTTGGAGCTCT+AGG - chr1.1:67506232-67506251 MS.gene005865:intron 35.0%
CATAGTATGGCGATAATATC+TGG - chr1.1:67504639-67504658 MS.gene005865:intron 35.0%
GAGTGGCAACTTATTGTTAT+TGG + chr1.1:67505396-67505415 None:intergenic 35.0%
GCGATAATATCTGGTTAGAT+TGG - chr1.1:67504648-67504667 MS.gene005865:intron 35.0%
TAATCCGGAGTTCAATCAAA+GGG + chr1.1:67506384-67506403 None:intergenic 35.0%
TATCCCTAATGAGTTTCATG+TGG - chr1.1:67504333-67504352 MS.gene005865:intron 35.0%
TCATCTGAATCTTCATCTTC+CGG + chr1.1:67506828-67506847 None:intergenic 35.0%
TCATTAATGACCAAGTGTCT+TGG + chr1.1:67506454-67506473 None:intergenic 35.0%
TCCAGCAAGAAATTTGATGT+GGG - chr1.1:67506165-67506184 MS.gene005865:intron 35.0%
TCTACCCAAAGGATAGAAAT+TGG + chr1.1:67504221-67504240 None:intergenic 35.0%
TGCGGAGTAACTAAACTAAA+AGG + chr1.1:67504411-67504430 None:intergenic 35.0%
TGCTATTATATAGACACGTG+CGG + chr1.1:67505201-67505220 None:intergenic 35.0%
TTAAAGGTAGAAAGATGCCA+GGG - chr1.1:67506089-67506108 MS.gene005865:intron 35.0%
TTTAAAGGTAGAAAGATGCC+AGG - chr1.1:67506088-67506107 MS.gene005865:intron 35.0%
TTTATGAACTTGGAGCTCTA+GGG - chr1.1:67506233-67506252 MS.gene005865:intron 35.0%
! AAAGGAAGTATAGATGTGTC+AGG + chr1.1:67506789-67506808 None:intergenic 35.0%
! AAGAAGTATGGATTTCATGG+AGG - chr1.1:67505857-67505876 MS.gene005865:CDS 35.0%
! GGTGTTATAGCTGTAAAATG+TGG - chr1.1:67504158-67504177 MS.gene005865:CDS 35.0%
! TGCGTTTCAAATTTTCAGAG+AGG + chr1.1:67505639-67505658 None:intergenic 35.0%
! TTCAAATTTTCAGAGAGGTC+AGG + chr1.1:67505634-67505653 None:intergenic 35.0%
! TTTTTGAATGGCAGTTACAC+GGG - chr1.1:67505794-67505813 MS.gene005865:intron 35.0%
!! GGTTGATGAGTTTGAGTTAA+AGG - chr1.1:67506307-67506326 MS.gene005865:intron 35.0%
!!! GCGATTTTTTAGTTCAGATG+TGG - chr1.1:67504076-67504095 MS.gene005865:CDS 35.0%
AAACCTTTGGTTGCTGATCT+TGG - chr1.1:67506852-67506871 MS.gene005865:CDS 40.0%
AACCTTTGGTTGCTGATCTT+GGG - chr1.1:67506853-67506872 MS.gene005865:CDS 40.0%
AACTGCAAATGTGCAAATGG+TGG + chr1.1:67506503-67506522 None:intergenic 40.0%
AAGACATATCTAGGTACTCC+AGG + chr1.1:67504784-67504803 None:intergenic 40.0%
AAGTATTCCTGTAGAACCCA+TGG + chr1.1:67505918-67505937 None:intergenic 40.0%
AATCTTGCACGTACAAGTTC+AGG - chr1.1:67505569-67505588 MS.gene005865:intron 40.0%
AATGTGGAATGACTGCTCAT+TGG - chr1.1:67504174-67504193 MS.gene005865:intron 40.0%
ACAGGAATCACCAAAGGAAA+AGG - chr1.1:67505053-67505072 MS.gene005865:intron 40.0%
ACCCACATCAAATTTCTTGC+TGG + chr1.1:67506169-67506188 None:intergenic 40.0%
ACTGCAAATGTGCAAATGGT+GGG + chr1.1:67506502-67506521 None:intergenic 40.0%
ACTGCTCATTGGGATAAATG+GGG - chr1.1:67504185-67504204 MS.gene005865:intron 40.0%
AGATGACAACATTGTCTCTC+AGG - chr1.1:67504238-67504257 MS.gene005865:intron 40.0%
AGGAATACTTGGTCCTTGTA+AGG - chr1.1:67505928-67505947 MS.gene005865:CDS 40.0%
ATACTTGGTCCTTGTAAGGT+TGG - chr1.1:67505932-67505951 MS.gene005865:CDS 40.0%
ATGGGTTCTACAGGAATACT+TGG - chr1.1:67505917-67505936 MS.gene005865:CDS 40.0%
ATGTGCTTTCGAATTCCCAT+GGG + chr1.1:67505891-67505910 None:intergenic 40.0%
ATGTGGAATGACTGCTCATT+GGG - chr1.1:67504175-67504194 MS.gene005865:intron 40.0%
ATTCAGCTTAGAGTTACTCC+TGG - chr1.1:67504763-67504782 MS.gene005865:CDS 40.0%
CAATGTTGTCATCTACCCAA+AGG + chr1.1:67504232-67504251 None:intergenic 40.0%
CAGGGTGTAATGAAGAAGTA+TGG - chr1.1:67505845-67505864 MS.gene005865:CDS 40.0%
CATACTTCTTCATTACACCC+TGG + chr1.1:67505847-67505866 None:intergenic 40.0%
CCAATATCAACCAAGACACT+TGG - chr1.1:67506441-67506460 MS.gene005865:intron 40.0%
CCATTTGCACATTTGCAGTT+CGG - chr1.1:67506503-67506522 MS.gene005865:intron 40.0%
CTCACTGTTCAGGTCTTTAA+AGG - chr1.1:67506073-67506092 MS.gene005865:intron 40.0%
CTTACCCTTTGATTGAACTC+CGG - chr1.1:67506377-67506396 MS.gene005865:intron 40.0%
GACTGCTCATTGGGATAAAT+GGG - chr1.1:67504184-67504203 MS.gene005865:intron 40.0%
GATTGAACTCCGGATTACTA+GGG - chr1.1:67506387-67506406 MS.gene005865:intron 40.0%
GCTATTATATAGACACGTGC+GGG + chr1.1:67505200-67505219 None:intergenic 40.0%
GTAATCCGGAGTTCAATCAA+AGG + chr1.1:67506385-67506404 None:intergenic 40.0%
GTTCAGATGTGGAGACTATA+AGG - chr1.1:67504087-67504106 MS.gene005865:CDS 40.0%
TATCATCAAGAGAACGATGG+TGG - chr1.1:67506591-67506610 MS.gene005865:intron 40.0%
TATGTGCTTTCGAATTCCCA+TGG + chr1.1:67505892-67505911 None:intergenic 40.0%
TCAGAGAGGTCAGGTAAAAT+GGG + chr1.1:67505625-67505644 None:intergenic 40.0%
TCTTGAAGCTTCCTCTTCAT+CGG + chr1.1:67504948-67504967 None:intergenic 40.0%
TGACTGCTCATTGGGATAAA+TGG - chr1.1:67504183-67504202 MS.gene005865:intron 40.0%
TGATTGAACTCCGGATTACT+AGG - chr1.1:67506386-67506405 MS.gene005865:intron 40.0%
TTCAGAGAGGTCAGGTAAAA+TGG + chr1.1:67505626-67505645 None:intergenic 40.0%
TTTCCCAACTTACTTTGTGC+CGG - chr1.1:67506806-67506825 MS.gene005865:CDS 40.0%
! AACGCAAAATGTTGAGTTGC+AGG - chr1.1:67505653-67505672 MS.gene005865:intron 40.0%
! CACCAAAGGAAAAGGTTTTC+AGG - chr1.1:67505061-67505080 MS.gene005865:intron 40.0%
! CACCTGAAAACCTTTTCCTT+TGG + chr1.1:67505066-67505085 None:intergenic 40.0%
! CCAAGTGTCTTGGTTGATAT+TGG + chr1.1:67506444-67506463 None:intergenic 40.0%
! TCGAAAGCACATAGAACCAT+GGG - chr1.1:67505899-67505918 MS.gene005865:CDS 40.0%
! TTATGAACTTGGAGCTCTAG+GGG - chr1.1:67506234-67506253 MS.gene005865:intron 40.0%
! TTCGAAAGCACATAGAACCA+TGG - chr1.1:67505898-67505917 MS.gene005865:CDS 40.0%
!! AATGTTCGCCATTTTGTTCC+TGG - chr1.1:67505014-67505033 MS.gene005865:CDS 40.0%
!! ATGTTCGCCATTTTGTTCCT+GGG - chr1.1:67505015-67505034 MS.gene005865:CDS 40.0%
!! GAATTTGGTGTCATCACACT+AGG + chr1.1:67504128-67504147 None:intergenic 40.0%
!! TAGCAGTTGAGTTGAATGCA+TGG - chr1.1:67505216-67505235 MS.gene005865:intron 40.0%
ACACTTGACAAAGCCTGAAG+TGG - chr1.1:67504899-67504918 MS.gene005865:intron 45.0%
ACCTTTGGTTGCTGATCTTG+GGG - chr1.1:67506854-67506873 MS.gene005865:CDS 45.0%
AGAAAGATGCCAGGGAGAAT+GGG - chr1.1:67506097-67506116 MS.gene005865:intron 45.0%
ATCAAGTGATTGAGGTCGAG+CGG - chr1.1:67506286-67506305 MS.gene005865:intron 45.0%
CACTTGACAAAGCCTGAAGT+GGG - chr1.1:67504900-67504919 MS.gene005865:intron 45.0%
CATACTGCCCAGGAACAAAA+TGG + chr1.1:67505025-67505044 None:intergenic 45.0%
CATAGAACCATGGGTTCTAC+AGG - chr1.1:67505908-67505927 MS.gene005865:CDS 45.0%
CCGAACTGCAAATGTGCAAA+TGG + chr1.1:67506506-67506525 None:intergenic 45.0%
CTTCCGGCACAAAGTAAGTT+GGG + chr1.1:67506812-67506831 None:intergenic 45.0%
GAACAATAGCCTTCCTTCTC+AGG + chr1.1:67504272-67504291 None:intergenic 45.0%
GAACTAAAAAATCGCGGCAG+TGG + chr1.1:67504071-67504090 None:intergenic 45.0%
GACATCACAGGAATCACCAA+AGG - chr1.1:67505047-67505066 MS.gene005865:CDS 45.0%
GCACGTACAAGTTCAGGTAA+TGG - chr1.1:67505575-67505594 MS.gene005865:intron 45.0%
GCGACAGTATCCGATTAGAT+TGG - chr1.1:67504702-67504721 MS.gene005865:intron 45.0%
GTCAAAACTCCTGAGAAGGA+AGG - chr1.1:67504260-67504279 MS.gene005865:intron 45.0%
TAGAAAGATGCCAGGGAGAA+TGG - chr1.1:67506096-67506115 MS.gene005865:intron 45.0%
TCAGGTCAAAACTCCTGAGA+AGG - chr1.1:67504256-67504275 MS.gene005865:intron 45.0%
TCTTCCGGCACAAAGTAAGT+TGG + chr1.1:67506813-67506832 None:intergenic 45.0%
TGGGGATGTGGATCCATTTA+TGG - chr1.1:67506872-67506891 MS.gene005865:CDS 45.0%
TTGGTCCTTGTAAGGTTGGA+TGG - chr1.1:67505936-67505955 MS.gene005865:CDS 45.0%
TTGTATGGTGTGCACAACAC+AGG - chr1.1:67506710-67506729 MS.gene005865:CDS 45.0%
! GATTGGTTGTGGACAGAAGA+AGG - chr1.1:67504872-67504891 MS.gene005865:intron 45.0%
! GGCACAAAGTAAGTTGGGAA+AGG + chr1.1:67506807-67506826 None:intergenic 45.0%
! GTTGAGTTGCAGGCTTTCTA+GGG - chr1.1:67505663-67505682 MS.gene005865:intron 45.0%
! TACTAGGGCTCCTTCCTTTT+GGG - chr1.1:67506402-67506421 MS.gene005865:intron 45.0%
! TCAAGAGTTTCGAGTGACAG+AGG - chr1.1:67504962-67504981 MS.gene005865:intron 45.0%
! TGTTGAGTTGCAGGCTTTCT+AGG - chr1.1:67505662-67505681 MS.gene005865:intron 45.0%
! TTACTAGGGCTCCTTCCTTT+TGG - chr1.1:67506401-67506420 MS.gene005865:intron 45.0%
! TTGAGTTGCAGGCTTTCTAG+GGG - chr1.1:67505664-67505683 MS.gene005865:intron 45.0%
!! AGCCTGAAGTGGGTCATTTT+AGG - chr1.1:67504910-67504929 MS.gene005865:intron 45.0%
!! GCCTGAAGTGGGTCATTTTA+GGG - chr1.1:67504911-67504930 MS.gene005865:intron 45.0%
!! TGATATTGGCCTTGACTCTC+CGG + chr1.1:67506430-67506449 None:intergenic 45.0%
ACAAAGTTCCCTGTAGCTCC+TGG + chr1.1:67506738-67506757 None:intergenic 50.0%
AGAATGAAGCAGAACGCACC+TGG + chr1.1:67504298-67504317 None:intergenic 50.0%
AGGAAGGAGCCCTAGTAATC+CGG + chr1.1:67506399-67506418 None:intergenic 50.0%
ATGGAGGTTCAGCATCCCAT+GGG - chr1.1:67505873-67505892 MS.gene005865:CDS 50.0%
CAAAGTTCCCTGTAGCTCCT+GGG + chr1.1:67506737-67506756 None:intergenic 50.0%
CTCAAGGAGTTCCGATGAAG+AGG - chr1.1:67504934-67504953 MS.gene005865:intron 50.0%
GAGGATGCACTTCTTCCTGT+TGG - chr1.1:67504981-67505000 MS.gene005865:CDS 50.0%
GCCCTAAAATGACCCACTTC+AGG + chr1.1:67504915-67504934 None:intergenic 50.0%
GGAAGGCTATTGTTCTCTCC+AGG - chr1.1:67504277-67504296 MS.gene005865:intron 50.0%
GGACTCCATCCAACCTTACA+AGG + chr1.1:67505944-67505963 None:intergenic 50.0%
GGGCAGTATGTTGACATCAC+AGG - chr1.1:67505035-67505054 MS.gene005865:CDS 50.0%
GTGATGTCAACATACTGCCC+AGG + chr1.1:67505035-67505054 None:intergenic 50.0%
GTGCGTTCTGCTTCATTCTC+AGG - chr1.1:67504299-67504318 MS.gene005865:intron 50.0%
TCCCCAAGATCAGCAACCAA+AGG + chr1.1:67506858-67506877 None:intergenic 50.0%
TGAGTTGCAGGCTTTCTAGG+GGG - chr1.1:67505665-67505684 MS.gene005865:intron 50.0%
TTGACTCTCCGGTTCCCAAA+AGG + chr1.1:67506419-67506438 None:intergenic 50.0%
TTTGATGTGGGTGAAAGGCC+AGG - chr1.1:67506177-67506196 MS.gene005865:intron 50.0%
! GGATTCAGGACTCTGAAGAG+TGG + chr1.1:67505413-67505432 None:intergenic 50.0%
!! TTTTGGGAACCGGAGAGTCA+AGG - chr1.1:67506418-67506437 MS.gene005865:intron 50.0%
!!! GTGGGTCATTTTAGGGCTCA+AGG - chr1.1:67504918-67504937 MS.gene005865:intron 50.0%
AAGATGCCAGGGAGAATGGG+TGG - chr1.1:67506100-67506119 MS.gene005865:intron 55.0%
AGATGCCAGGGAGAATGGGT+GGG - chr1.1:67506101-67506120 MS.gene005865:intron 55.0%
CATGGAGGTTCAGCATCCCA+TGG - chr1.1:67505872-67505891 MS.gene005865:CDS 55.0%
CTCTCCGGTTCCCAAAAGGA+AGG + chr1.1:67506415-67506434 None:intergenic 55.0%
GGTTGCTGATCTTGGGGATG+TGG - chr1.1:67506860-67506879 MS.gene005865:CDS 55.0%
TCGTCTTCGACAATTCCCCG+TGG - chr1.1:67504043-67504062 MS.gene005865:CDS 55.0%
! GAGTTGCAGGCTTTCTAGGG+GGG - chr1.1:67505666-67505685 MS.gene005865:intron 55.0%
!! GCTGGCGTTTTCAAGAGGTG+TGG - chr1.1:67504016-67504035 MS.gene005865:CDS 55.0%
!! GGCTCCTTCCTTTTGGGAAC+CGG - chr1.1:67506408-67506427 MS.gene005865:intron 55.0%
ACACAGGTCCCAGGAGCTAC+AGG - chr1.1:67506726-67506745 MS.gene005865:CDS 60.0%
CACAGGTCCCAGGAGCTACA+GGG - chr1.1:67506727-67506746 MS.gene005865:CDS 60.0%
GTGTGCACAACACAGGTCCC+AGG - chr1.1:67506717-67506736 MS.gene005865:CDS 60.0%
TGCTTCCCACCCATTCTCCC+TGG + chr1.1:67506109-67506128 None:intergenic 60.0%
! AAATCGCGGCAGTGGTGCCA+CGG + chr1.1:67504063-67504082 None:intergenic 60.0%
!! AATCGCGGCAGTGGTGCCAC+GGG + chr1.1:67504062-67504081 None:intergenic 65.0%
!! ATCGCGGCAGTGGTGCCACG+GGG + chr1.1:67504061-67504080 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 67504014 67506905 67504014 ID=MS.gene005865
chr1.1 mRNA 67504014 67506905 67504014 ID=MS.gene005865.t1;Parent=MS.gene005865
chr1.1 exon 67506621 67506905 67506621 ID=MS.gene005865.t1.exon1;Parent=MS.gene005865.t1
chr1.1 CDS 67506621 67506905 67506621 ID=cds.MS.gene005865.t1;Parent=MS.gene005865.t1
chr1.1 exon 67505837 67506046 67505837 ID=MS.gene005865.t1.exon2;Parent=MS.gene005865.t1
chr1.1 CDS 67505837 67506046 67505837 ID=cds.MS.gene005865.t1;Parent=MS.gene005865.t1
chr1.1 exon 67504970 67505071 67504970 ID=MS.gene005865.t1.exon3;Parent=MS.gene005865.t1
chr1.1 CDS 67504970 67505071 67504970 ID=cds.MS.gene005865.t1;Parent=MS.gene005865.t1
chr1.1 exon 67504721 67504834 67504721 ID=MS.gene005865.t1.exon4;Parent=MS.gene005865.t1
chr1.1 CDS 67504721 67504834 67504721 ID=cds.MS.gene005865.t1;Parent=MS.gene005865.t1
chr1.1 exon 67504014 67504187 67504014 ID=MS.gene005865.t1.exon5;Parent=MS.gene005865.t1
chr1.1 CDS 67504014 67504187 67504014 ID=cds.MS.gene005865.t1;Parent=MS.gene005865.t1
Gene Sequence

>MS.gene005865

ATGCTGGCGTTTTCAAGAGGTGTGGTTTCTCGTCTTCGACAATTCCCCGTGGCACCACTGCCGCGATTTTTTAGTTCAGATGTGGAGACTATAAGGACGATTGAAGCAAAACCTAGTGTGATGACACCAAATTCACAAAGAACTGGTGTTATAGCTGTAAAATGTGGAATGACTGCTCATTGGGATAAATGGGGTGCTAGAATTCCAATTTCTATCCTTTGGGTAGATGACAACATTGTCTCTCAGGTCAAAACTCCTGAGAAGGAAGGCTATTGTTCTCTCCAGGTGCGTTCTGCTTCATTCTCAGGATCAAATTCAATATCCCTAATGAGTTTCATGTGGATTAACTCAGTTGAGAGTTACTTATTGAATTACTTAAGTTACTGCGACAATACCTTTTAGTTTAGTTACTCCGCATTATTCATTATAAACATTGAATTTCCGAAATCCAATCAACTACACAAATTTTCGAAAGAAATTAAAACTATTTGAAAGTTCATTAAAGAACTTCAAATGAACGTATAGTTATTTATTTTATTTTTTTAAATGCATGTAAACATCGTCTGCTAGATTGCATAAATACAAATTCTGGAATTATATGAAACTATGTGTACTTGATCCTTAACATAGTATGGCGATAATATCTGGTTAGATTGGTGAAATAATACAGTAAAAAAAGCAGAATATGGCGACAGTATCCGATTAGATTGGTGAAATTCAATGTTTATAATGAGTTATTAAATGGAATAATTCAGCTTAGAGTTACTCCTGGAGTACCTAGATATGTCTTCTATATATATGTATATAAAGTTAAATTTGTATCTAATTATAGACATGTTTGATGCTTTGTTTTAGTTAGATTGGTTGTGGACAGAAGAAGGAAAAACACTTGACAAAGCCTGAAGTGGGTCATTTTAGGGCTCAAGGAGTTCCGATGAAGAGGAAGCTTCAAGAGTTTCGAGTGACAGAGGATGCACTTCTTCCTGTTGGTACATCACTTAATGTTCGCCATTTTGTTCCTGGGCAGTATGTTGACATCACAGGAATCACCAAAGGAAAAGGTTTTCAGGTGTTTTTCTTTTTCCCTCGTATTACATACTTTCATATTGTTTTCCATATAAATAATAATTTCTGTCTTACATTGAGTATGTAGCATGTGTACATGTTTATGCTTGTATGTGGTCCCGCACGTGTCTATATAATAGCAGTTGAGTTGAATGCATGGTGTTCTTCGTTTGTTTATTGACATGATTGGTTATTATTTTGTCAAAGATTTTATTAAATTTGTATTTGTAGGATTAAAATGAATTACCCTAAGATGATTGTTTTTTTGTATGGGCTAGTTGATTACATTAGTTATATTAGGATTTTAAGTGTTGCCAATAACAATAAGTTGCCACTCTTCAGAGTCCTGAATCCTAATGATATAATAAGTTGACAAATTCAATCATGTATTATAGGCCATTATTTATTGTTTTGTTCAATTGTTTTCTGTTTCACGATTTATTTACTTCTAGACAATCATTGGTGTTCATTAGTTTCAACTTTCAACTATAATCTTGCACGTACAAGTTCAGGTAATGGTGCAACTTTATTCTTTTCTCTCTTCCCATTTTACCTGACCTCTCTGAAAATTTGAAACGCAAAATGTTGAGTTGCAGGCTTTCTAGGGGGGCCATTTATTGTTTATTTTTCTGCTTCAAAATGTATTTGTCATTCATGTATGATTGCGGATACTTCTCTGTTTCATGTTGAAACCTTGTCAGTTACACGTTTTTTTTTTTTGAATGGCAGTTACACGGGAGTTAATTTAAATTATATTTTCTGTTTCCAGGGTGTAATGAAGAAGTATGGATTTCATGGAGGTTCAGCATCCCATGGGAATTCGAAAGCACATAGAACCATGGGTTCTACAGGAATACTTGGTCCTTGTAAGGTTGGATGGAGTCCTTCAGATTAGTATTCTTAGTTAATACAGCTTTCTCTATTTGTACGTATTGAAATCATGTTAGATTGACTGTCTGTATAAGTTTATTTTCTTTGCATTACATTTATGTTTCTCACTGTTCAGGTCTTTAAAGGTAGAAAGATGCCAGGGAGAATGGGTGGGAAGCAAAGAACAGTAAAAAATGTATGGGTCTACAAAATTGATCCAGCAAGAAATTTGATGTGGGTGAAAGGCCAGGTAATTGTTTTTACATGTTTATCATTCACATTAATTTATGAACTTGGAGCTCTAGGGGAGTACTATCTTATATCACCTCATACACAAATCAAGTGATTGAGGTCGAGCGGTTGATGAGTTTGAGTTAAAGGATGAATCAATCATATTCATGGCTGAAACAATCCATGTTAAAACTTTACTTACCCTTTGATTGAACTCCGGATTACTAGGGCTCCTTCCTTTTGGGAACCGGAGAGTCAAGGCCAATATCAACCAAGACACTTGGTCATTAATGATTTTAAATTTTTATTAAATTTGAATCCCACCATTTGCACATTTGCAGTTCGGCTAGTACAGAGACTTGTATGTTTGTGTGAGCCAAGAAATTTTGTACATCAAAAAATTCTGACTAATATCATCAAGAGAACGATGGTGGATTGAAACCATTTTGTATTTAATTTAGTAGCTCAATTATTCATTAACTGGCAAAAATATTTGAAATCTCGGATTTATCACGGAATGCTTATTCTCTTTGTATGGTGTGCACAACACAGGTCCCAGGAGCTACAGGGAACTTTGTTTTTATAAAAGATGCAGTCTTTGAAAAACCTGACACATCTATACTTCCTTTCCCAACTTACTTTGTGCCGGAAGATGAAGATTCAGATGATATGAAACCTTTGGTTGCTGATCTTGGGGATGTGGATCCATTTATGGTTACTGATTAA
Protein sequence

>MS.gene005865.t1

MLAFSRGVVSRLRQFPVAPLPRFFSSDVETIRTIEAKPSVMTPNSQRTGVIAVKCGMTAHWDKWGARIPISILWVDDNIVSQVKTPEKEGYCSLQIGCGQKKEKHLTKPEVGHFRAQGVPMKRKLQEFRVTEDALLPVGTSLNVRHFVPGQYVDITGITKGKGFQGVMKKYGFHGGSASHGNSKAHRTMGSTGILGPCKVFKGRKMPGRMGGKQRTVKNVWVYKIDPARNLMWVKGQVPGATGNFVFIKDAVFEKPDTSILPFPTYFVPEDEDSDDMKPLVADLGDVDPFMVTD