AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066059

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066059.t1 MTR_1g031890 97.857 280 6 0 1 280 1 280 0.0 551
MS.gene066059.t1 MTR_1g085490 97.500 280 7 0 1 280 1 280 0.0 549
MS.gene066059.t1 MTR_1g031890 97.834 277 6 0 1 277 1 277 0.0 546
MS.gene066059.t1 MTR_4g118060 96.786 280 9 0 1 280 1 280 0.0 544
MS.gene066059.t1 MTR_1g031890 97.423 194 5 0 1 194 1 194 1.34e-136 384
MS.gene066059.t1 MTR_1g110750 31.977 172 102 7 74 233 78 246 9.32e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066059.t1 AT5G64670 73.646 277 56 5 1 268 1 269 1.89e-138 392
MS.gene066059.t1 AT3G25920 30.667 225 128 11 5 211 19 233 7.84e-13 67.4

Find 91 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTAAAACCTTGAAAATT+TGG 0.097234 1.2:+58587519 None:intergenic
TTACAATAAGTTAGGCTTTC+GGG 0.235237 1.2:-58585364 MS.gene066059:CDS
CCCCAACTTTGAACCTTTCC+TGG 0.235844 1.2:+58587371 None:intergenic
ACCTGAAAAGTGAGGCTAAA+TGG 0.251841 1.2:+58587291 None:intergenic
GGAGGAGGTCTAGCTGCTTT+AGG 0.302384 1.2:+58585297 None:intergenic
CTCTAAAACCTTGAAAATTT+GGG 0.308415 1.2:+58587520 None:intergenic
AGCCGCTTCAACTGCTTCCT+TGG 0.345478 1.2:+58585410 None:intergenic
TGAAAATTTGGGTGAGCATT+GGG 0.346095 1.2:+58587531 None:intergenic
ATTACAATAAGTTAGGCTTT+CGG 0.353200 1.2:-58585365 MS.gene066059:CDS
TTGAACCTTTCCTGGCTCTC+TGG 0.363875 1.2:+58587379 None:intergenic
GTCACAAAGGCCAGAGAGCC+AGG 0.365783 1.2:-58587389 MS.gene066059:CDS
ACACAGGATGCAGGGGTACT+TGG 0.366534 1.2:-58586103 MS.gene066059:intron
GCCAGGAAAGGTTCAAAGTT+GGG 0.370354 1.2:-58587372 MS.gene066059:CDS
ATACATACCTCTAGATGAAT+TGG 0.374426 1.2:+58586022 None:intergenic
AAGGTGTATTACAATAAGTT+AGG 0.375769 1.2:-58585372 MS.gene066059:CDS
GGAGCTGCAAGGGATGAAAT+TGG 0.382447 1.2:+58587586 None:intergenic
CCTGAAAAGTGAGGCTAAAT+GGG 0.402843 1.2:+58587292 None:intergenic
ACAAGGAAAGGTCGTGGTAT+TGG 0.409504 1.2:-58587441 MS.gene066059:CDS
TTGAAAATTTGGGTGAGCAT+TGG 0.414339 1.2:+58587530 None:intergenic
TCCTACGGCGAAGAGGGGTT+TGG 0.422318 1.2:+58587337 None:intergenic
GGGTTCTTGAAACCACGTTT+AGG 0.422852 1.2:+58587312 None:intergenic
ATTGCAACATTTATCAATGC+TGG 0.438724 1.2:-58586253 MS.gene066059:CDS
AGGTAACAAGGGTCACTGTC+AGG 0.442200 1.2:-58585434 MS.gene066059:CDS
ATTATCTCTCAGATCATTCA+AGG 0.446007 1.2:+58587482 None:intergenic
AAAGGTCGTGGTATTGGATC+TGG 0.457769 1.2:-58587435 MS.gene066059:CDS
TAAAGATGGAGTAAGATTGA+TGG 0.459426 1.2:-58586071 MS.gene066059:CDS
TCTGGAAAGGGAAAGACTGC+TGG 0.460358 1.2:-58587417 MS.gene066059:CDS
ACTCACAACAAATCCGTTCT+CGG 0.463672 1.2:-58587621 MS.gene066059:CDS
GGAATTGGCTTCTTCGGAGC+AGG 0.465181 1.2:+58585237 None:intergenic
AAAGGGAAAGACTGCTGGGA+GGG 0.483006 1.2:-58587412 MS.gene066059:CDS
CCTTTCTTCTCAAACCACTC+AGG 0.485963 1.2:+58585330 None:intergenic
AAAGATGGAGTAAGATTGAT+GGG 0.489075 1.2:-58586070 MS.gene066059:CDS
AGCCAGGAAAGGTTCAAAGT+TGG 0.492590 1.2:-58587373 MS.gene066059:CDS
CTTGGGAAGCAAATTAAAGA+TGG 0.493440 1.2:-58586085 MS.gene066059:CDS
ATTTAACTGAGCTGACCTAC+TGG 0.497431 1.2:+58585186 None:intergenic
AAGGATAAAGTTGATAGCAT+TGG 0.500169 1.2:-58585267 MS.gene066059:CDS
TATTACCATACACAGGATGC+AGG 0.500174 1.2:-58586112 MS.gene066059:intron
GAAGAAAGATGCTAAGAAGA+AGG 0.501124 1.2:-58587680 None:intergenic
CACAGGATGCAGGGGTACTT+GGG 0.501363 1.2:-58586102 MS.gene066059:intron
TAGACCTCCTCCAAAACAAA+AGG 0.503344 1.2:-58585286 MS.gene066059:CDS
GGCTGGTGGGTCCGTCAGAA+AGG 0.508668 1.2:-58585391 MS.gene066059:CDS
TAGGCATCCTACGGCGAAGA+GGG 0.511475 1.2:+58587331 None:intergenic
CTGGAAAGGGAAAGACTGCT+GGG 0.513550 1.2:-58587416 MS.gene066059:CDS
AAGGAAGCAGTTGAAGCGGC+TGG 0.517828 1.2:-58585408 MS.gene066059:CDS
CCTGAGTGGTTTGAGAAGAA+AGG 0.522455 1.2:-58585330 MS.gene066059:CDS
CCTCTAGATGAATTGGCCAT+TGG 0.524840 1.2:+58586029 None:intergenic
CGTGGTATTGGATCTGGAAA+GGG 0.539393 1.2:-58587429 MS.gene066059:CDS
AGGGATGAAATTGGATAGAC+GGG 0.542610 1.2:+58587595 None:intergenic
GGTTCAAAGTTGGGGTTCGA+AGG 0.550775 1.2:-58587363 MS.gene066059:CDS
AAGGGAAAGACTGCTGGGAG+GGG 0.550921 1.2:-58587411 MS.gene066059:CDS
GCTGACCTACTGGAAGATCC+TGG 0.552817 1.2:+58585196 None:intergenic
AAGGGATGAAATTGGATAGA+CGG 0.557057 1.2:+58587594 None:intergenic
GATAGACGGGTATCCGAGAA+CGG 0.557179 1.2:+58587608 None:intergenic
CCAGGAAAGGTTCAAAGTTG+GGG 0.558197 1.2:-58587371 MS.gene066059:CDS
TCGTGGTATTGGATCTGGAA+AGG 0.561440 1.2:-58587430 MS.gene066059:CDS
AAAGAGTGAGGGAGCTGCAA+GGG 0.562238 1.2:+58587576 None:intergenic
AAGGGTCACTGTCAGGGCCA+AGG 0.567491 1.2:-58585427 MS.gene066059:CDS
GGAGTAAGATTGATGGGGCG+TGG 0.570786 1.2:-58586064 MS.gene066059:CDS
GATCACAATGAAAACACTCA+AGG 0.573417 1.2:-58586209 MS.gene066059:intron
TATTGAGTTTACAGGTAACA+AGG 0.577340 1.2:-58585446 MS.gene066059:intron
GAAGCAGTTGAAGCGGCTGG+TGG 0.577528 1.2:-58585405 MS.gene066059:CDS
GGCCAAGGAAGCAGTTGAAG+CGG 0.578446 1.2:-58585412 MS.gene066059:CDS
AAGCAGTTGAAGCGGCTGGT+GGG 0.580431 1.2:-58585404 MS.gene066059:CDS
ATTGTAATACACCTTTCTGA+CGG 0.584521 1.2:+58585380 None:intergenic
GGTAACAAGGGTCACTGTCA+GGG 0.585974 1.2:-58585433 MS.gene066059:CDS
CGAAAACAGAAGACAAGGAA+AGG 0.589508 1.2:-58587453 MS.gene066059:CDS
TCAAAGTTGGGGTTCGAAGG+AGG 0.591062 1.2:-58587360 MS.gene066059:CDS
ATTACCATACACAGGATGCA+GGG 0.600741 1.2:-58586111 MS.gene066059:intron
AAAGGCCAGAGAGCCAGGAA+AGG 0.600747 1.2:-58587384 MS.gene066059:CDS
CAGAAGACAAGGAAAGGTCG+TGG 0.605789 1.2:-58587447 MS.gene066059:CDS
AGGCATCCTACGGCGAAGAG+GGG 0.607244 1.2:+58587332 None:intergenic
CCAATGGCCAATTCATCTAG+AGG 0.608787 1.2:-58586029 MS.gene066059:intron
GCACGAACGTTGAAAGAGTG+AGG 0.615232 1.2:+58587564 None:intergenic
TTAGGCATCCTACGGCGAAG+AGG 0.618508 1.2:+58587330 None:intergenic
GAAAGAGTGAGGGAGCTGCA+AGG 0.621872 1.2:+58587575 None:intergenic
CGCCGTAGGATGCCTAAACG+TGG 0.624469 1.2:-58587324 MS.gene066059:CDS
AACCACGTTTAGGCATCCTA+CGG 0.626355 1.2:+58587322 None:intergenic
GTGGTTCTGAGAAGATCCAA+TGG 0.632368 1.2:-58586045 MS.gene066059:CDS
ATTGAGTTTACAGGTAACAA+GGG 0.639306 1.2:-58585445 MS.gene066059:intron
GAAAGGGAAAGACTGCTGGG+AGG 0.641614 1.2:-58587413 MS.gene066059:CDS
TTCCCCGAAAACAGAAGACA+AGG 0.642420 1.2:-58587458 MS.gene066059:CDS
GGGCACTGTTGAAGCCTGAG+TGG 0.644311 1.2:-58585344 MS.gene066059:CDS
GCCAAACCCCTCTTCGCCGT+AGG 0.645837 1.2:-58587338 MS.gene066059:CDS
CACGAACGTTGAAAGAGTGA+GGG 0.652354 1.2:+58587565 None:intergenic
GGAAGCCAGGATCTTCCAGT+AGG 0.653803 1.2:-58585201 MS.gene066059:CDS
ACTGCTGGGAGGGGTCACAA+AGG 0.661736 1.2:-58587402 MS.gene066059:CDS
AGAAGTATACCTGAAAAGTG+AGG 0.669464 1.2:+58587283 None:intergenic
ATAAAGTTGATAGCATTGGA+CGG 0.672998 1.2:-58585263 MS.gene066059:CDS
GTGAGCATTGGGATTAAATG+TGG 0.673885 1.2:+58587542 None:intergenic
TTACCATACACAGGATGCAG+GGG 0.679526 1.2:-58586110 MS.gene066059:intron
AAGATGGAGTAAGATTGATG+GGG 0.737220 1.2:-58586069 MS.gene066059:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAATATTCAAAACTAT+AGG + chr1.2:58585756-58585775 None:intergenic 10.0%
!!! ATAATTTTTAATAATATTCC+TGG + chr1.2:58585919-58585938 None:intergenic 10.0%
!! AACAAAAAAGATATATAAAC+AGG + chr1.2:58585604-58585623 None:intergenic 15.0%
!! GAGATAAATAAATGTAAAAT+TGG - chr1.2:58587308-58587327 MS.gene066059:CDS 15.0%
!!! AAAGTATTTCTAAAGATTTA+AGG + chr1.2:58586923-58586942 None:intergenic 15.0%
!!! AAGATAAGATTTTTATTTCA+AGG - chr1.2:58586023-58586042 MS.gene066059:intron 15.0%
!!! ATTATAATTCTGAGATTTAT+GGG - chr1.2:58585768-58585787 MS.gene066059:intron 15.0%
!!! TATTATAATTCTGAGATTTA+TGG - chr1.2:58585767-58585786 MS.gene066059:intron 15.0%
!! AAATCTTCTGTAAACATATA+TGG - chr1.2:58586052-58586071 MS.gene066059:CDS 20.0%
!! ATAATAAACAAAAGAATGAG+AGG + chr1.2:58586728-58586747 None:intergenic 20.0%
!! TAATAAACAAAAGAATGAGA+GGG + chr1.2:58586727-58586746 None:intergenic 20.0%
!! TGAACTCTAATAATATACTA+AGG + chr1.2:58585713-58585732 None:intergenic 20.0%
!! TTATAACTTATTGAGTTTAC+AGG - chr1.2:58587406-58587425 MS.gene066059:CDS 20.0%
!! TTATTATTATTACCATACAC+AGG - chr1.2:58586741-58586760 MS.gene066059:intron 20.0%
!!! AAACAAATTCATTTTAAGTG+AGG + chr1.2:58586692-58586711 None:intergenic 20.0%
!!! TTTATTGTATTGACATTTGA+CGG - chr1.2:58586200-58586219 MS.gene066059:intron 20.0%
!!! TTTTCAATTCTAAACACTTA+AGG - chr1.2:58587336-58587355 MS.gene066059:CDS 20.0%
! AAACTTTATAGGCATATAAG+AGG - chr1.2:58587379-58587398 MS.gene066059:CDS 25.0%
! AACTTTATAGGCATATAAGA+GGG - chr1.2:58587380-58587399 MS.gene066059:CDS 25.0%
! AAGGATAAAGAACAAAATGT+TGG - chr1.2:58587111-58587130 MS.gene066059:intron 25.0%
! AAGGTGTATTACAATAAGTT+AGG - chr1.2:58587488-58587507 MS.gene066059:CDS 25.0%
! AATGTCTAGATAAGTTTATG+TGG - chr1.2:58586419-58586438 MS.gene066059:intron 25.0%
! AATTGACATGTAGAAATAAG+AGG + chr1.2:58586114-58586133 None:intergenic 25.0%
! ATAATATGACTGATAGATTG+AGG + chr1.2:58586526-58586545 None:intergenic 25.0%
! ATTACAATAAGTTAGGCTTT+CGG - chr1.2:58587495-58587514 MS.gene066059:CDS 25.0%
! CTAAAGATTTAAGGATCAAA+AGG + chr1.2:58586914-58586933 None:intergenic 25.0%
! GAGTTCATTAGTTATAATTG+TGG - chr1.2:58585726-58585745 MS.gene066059:intron 25.0%
! TAAAATTTCTGTAGTGTCTA+AGG - chr1.2:58587092-58587111 MS.gene066059:intron 25.0%
! TAATAATATTCCTGGAGAAA+TGG + chr1.2:58585911-58585930 None:intergenic 25.0%
! TATACAGTCACAAACTTTAT+AGG - chr1.2:58587368-58587387 MS.gene066059:CDS 25.0%
!!! CTAAGTAATAAGTGTTTTTG+AGG - chr1.2:58586876-58586895 MS.gene066059:intron 25.0%
!!! CTCTAAAACCTTGAAAATTT+GGG + chr1.2:58585343-58585362 None:intergenic 25.0%
!!! GTATGTATACTTTGATTTGA+AGG - chr1.2:58586853-58586872 MS.gene066059:intron 25.0%
!!! TCAACTTTATCCTTTTGTTT+TGG + chr1.2:58587587-58587606 None:intergenic 25.0%
!!! TTTTGAGCTTATTTTGATAC+TGG - chr1.2:58586975-58586994 MS.gene066059:intron 25.0%
!!! TTTTTAGTGCATATCATACT+TGG + chr1.2:58586138-58586157 None:intergenic 25.0%
AAAGATGGAGTAAGATTGAT+GGG - chr1.2:58586790-58586809 MS.gene066059:intron 30.0%
ACTGATAGATTGAGGAATAA+AGG + chr1.2:58586518-58586537 None:intergenic 30.0%
ACTTTATAGGCATATAAGAG+GGG - chr1.2:58587381-58587400 MS.gene066059:CDS 30.0%
ATACATACCTCTAGATGAAT+TGG + chr1.2:58586841-58586860 None:intergenic 30.0%
ATGTTGCCAATGTTAAAACA+TGG + chr1.2:58587062-58587081 None:intergenic 30.0%
ATTATCTCTCAGATCATTCA+AGG + chr1.2:58585381-58585400 None:intergenic 30.0%
ATTCTAAACACTTAAGGTGT+AGG - chr1.2:58587342-58587361 MS.gene066059:CDS 30.0%
ATTCTTCTGTGTATTTAACC+GGG - chr1.2:58585635-58585654 MS.gene066059:intron 30.0%
ATTGAGTTTACAGGTAACAA+GGG - chr1.2:58587415-58587434 MS.gene066059:CDS 30.0%
ATTGCAACATTTATCAATGC+TGG - chr1.2:58586607-58586626 MS.gene066059:intron 30.0%
ATTGTAATACACCTTTCTGA+CGG + chr1.2:58587483-58587502 None:intergenic 30.0%
CACACACAGTTAAAATACAT+TGG + chr1.2:58586233-58586252 None:intergenic 30.0%
CCATGCTTAATGCAAATATT+AGG - chr1.2:58585971-58585990 MS.gene066059:intron 30.0%
CCTAATATTTGCATTAAGCA+TGG + chr1.2:58585974-58585993 None:intergenic 30.0%
CCTTCAACTAAAAATGGAAT+TGG + chr1.2:58587641-58587660 None:intergenic 30.0%
CTGATAGATTGAGGAATAAA+GGG + chr1.2:58586517-58586536 None:intergenic 30.0%
GATTCTTCTGTGTATTTAAC+CGG - chr1.2:58585634-58585653 MS.gene066059:intron 30.0%
TAAAGATGGAGTAAGATTGA+TGG - chr1.2:58586789-58586808 MS.gene066059:intron 30.0%
TATTGAGTTTACAGGTAACA+AGG - chr1.2:58587414-58587433 MS.gene066059:CDS 30.0%
TCAAGCAGAATAGTCTTTAT+TGG + chr1.2:58585867-58585886 None:intergenic 30.0%
TGATAGATTGAGGAATAAAG+GGG + chr1.2:58586516-58586535 None:intergenic 30.0%
TTACAATAAGTTAGGCTTTC+GGG - chr1.2:58587496-58587515 MS.gene066059:CDS 30.0%
TTTAACATTGGCAACATGAA+TGG - chr1.2:58587065-58587084 MS.gene066059:intron 30.0%
! AAAAATGGAATTGGCTTCTT+CGG + chr1.2:58587632-58587651 None:intergenic 30.0%
! AATGGTTTTCTTTCTTGGAA+TGG + chr1.2:58585893-58585912 None:intergenic 30.0%
! AATTCTGAAAAAGTCTCAGA+TGG + chr1.2:58585823-58585842 None:intergenic 30.0%
! TTTCTTCTTTTACAGCCAAT+AGG - chr1.2:58586574-58586593 MS.gene066059:intron 30.0%
!! AAGAACAAAATGTTGGCTTT+AGG - chr1.2:58587118-58587137 MS.gene066059:intron 30.0%
!! AAGGATAAAGTTGATAGCAT+TGG - chr1.2:58587593-58587612 MS.gene066059:CDS 30.0%
!! ATAAAGTTGATAGCATTGGA+CGG - chr1.2:58587597-58587616 MS.gene066059:CDS 30.0%
!! ATAATTCTGAGATTTATGGG+TGG - chr1.2:58585771-58585790 MS.gene066059:intron 30.0%
!! ATTCATTTTAAGTGAGGAAG+CGG + chr1.2:58586686-58586705 None:intergenic 30.0%
!! GCTCTAAAACCTTGAAAATT+TGG + chr1.2:58585344-58585363 None:intergenic 30.0%
!!! ACTTTATCCTTTTGTTTTGG+AGG + chr1.2:58587584-58587603 None:intergenic 30.0%
!!! CCAATTCCATTTTTAGTTGA+AGG - chr1.2:58587638-58587657 MS.gene066059:CDS 30.0%
AAGATGGAGTAAGATTGATG+GGG - chr1.2:58586791-58586810 MS.gene066059:intron 35.0%
AAGGGATGAAATTGGATAGA+CGG + chr1.2:58585269-58585288 None:intergenic 35.0%
AGAAAGAAAACCATTTCTCC+AGG - chr1.2:58585898-58585917 MS.gene066059:intron 35.0%
ATGGTTTACACTTGCCAAAA+GGG + chr1.2:58587024-58587043 None:intergenic 35.0%
CAAGTGTAAACCATCAAAAG+TGG - chr1.2:58587030-58587049 MS.gene066059:intron 35.0%
CTTGACACTCATAAATCTGA+AGG + chr1.2:58586321-58586340 None:intergenic 35.0%
CTTGGGAAGCAAATTAAAGA+TGG - chr1.2:58586775-58586794 MS.gene066059:intron 35.0%
GAGTGTCAAGTAACTTCTAA+GGG - chr1.2:58586331-58586350 MS.gene066059:intron 35.0%
GATCACAATGAAAACACTCA+AGG - chr1.2:58586651-58586670 MS.gene066059:intron 35.0%
TGAAAATTTGGGTGAGCATT+GGG + chr1.2:58585332-58585351 None:intergenic 35.0%
TGAGTGTCAAGTAACTTCTA+AGG - chr1.2:58586330-58586349 MS.gene066059:intron 35.0%
TGGCTTCCTTCAACTAAAAA+TGG + chr1.2:58587647-58587666 None:intergenic 35.0%
TGGTTACTTGTGAGCAATAA+TGG - chr1.2:58585791-58585810 MS.gene066059:intron 35.0%
TGTAAACATATATGGCTGAG+TGG - chr1.2:58586060-58586079 MS.gene066059:CDS 35.0%
TTGAAAATTTGGGTGAGCAT+TGG + chr1.2:58585333-58585352 None:intergenic 35.0%
! AATGCTCACCCAAATTTTCA+AGG - chr1.2:58585332-58585351 MS.gene066059:CDS 35.0%
! AGAAGTATACCTGAAAAGTG+AGG + chr1.2:58585580-58585599 None:intergenic 35.0%
! CTTTCCTTGTCTTCTGTTTT+CGG + chr1.2:58585409-58585428 None:intergenic 35.0%
! CTTTTACAGCCAATAGGTTT+GGG - chr1.2:58586580-58586599 MS.gene066059:intron 35.0%
! GCAATTTTTCCCAAACCTAT+TGG + chr1.2:58586592-58586611 None:intergenic 35.0%
! GGAGAAATGGTTTTCTTTCT+TGG + chr1.2:58585898-58585917 None:intergenic 35.0%
! TCTTTTACAGCCAATAGGTT+TGG - chr1.2:58586579-58586598 MS.gene066059:intron 35.0%
! TTTCCTTGTCTTCTGTTTTC+GGG + chr1.2:58585408-58585427 None:intergenic 35.0%
!! ATAAGTGTTTTTGAGGACCA+CGG - chr1.2:58586883-58586902 MS.gene066059:intron 35.0%
!! TCGATTTTGCTATGAAGAGT+TGG - chr1.2:58587151-58587170 MS.gene066059:intron 35.0%
!!! ATTTTTAGTTGAAGGAAGCC+AGG - chr1.2:58587646-58587665 MS.gene066059:CDS 35.0%
!!! TGACAGCCATGTTTTAACAT+TGG - chr1.2:58587053-58587072 MS.gene066059:intron 35.0%
!!! TTATCCTTTTGTTTTGGAGG+AGG + chr1.2:58587581-58587600 None:intergenic 35.0%
!!! TTTTAGAGCTTACAGTCTTC+TGG - chr1.2:58585355-58585374 MS.gene066059:CDS 35.0%
AAAGATACGAGCTTGAACCA+CGG + chr1.2:58586469-58586488 None:intergenic 40.0%
ACCTGAAAAGTGAGGCTAAA+TGG + chr1.2:58585572-58585591 None:intergenic 40.0%
ACTCACAACAAATCCGTTCT+CGG - chr1.2:58585239-58585258 MS.gene066059:CDS 40.0%
AGATTGAGGAATAAAGGGGA+AGG + chr1.2:58586512-58586531 None:intergenic 40.0%
AGCAAACATTAGGTAGCCAA+TGG + chr1.2:58585668-58585687 None:intergenic 40.0%
AGGGATGAAATTGGATAGAC+GGG + chr1.2:58585268-58585287 None:intergenic 40.0%
ATTACCATACACAGGATGCA+GGG - chr1.2:58586749-58586768 MS.gene066059:intron 40.0%
CCTGAAAAGTGAGGCTAAAT+GGG + chr1.2:58585571-58585590 None:intergenic 40.0%
CGAAAACAGAAGACAAGGAA+AGG - chr1.2:58585407-58585426 MS.gene066059:CDS 40.0%
GATGGTTTACACTTGCCAAA+AGG + chr1.2:58587025-58587044 None:intergenic 40.0%
GCCAAAGTGAACTACACATT+TGG + chr1.2:58586287-58586306 None:intergenic 40.0%
GCCAAATGTGTAGTTCACTT+TGG - chr1.2:58586283-58586302 MS.gene066059:intron 40.0%
TAGACCTCCTCCAAAACAAA+AGG - chr1.2:58587574-58587593 MS.gene066059:CDS 40.0%
TATTACCATACACAGGATGC+AGG - chr1.2:58586748-58586767 MS.gene066059:intron 40.0%
TTCAGCTTCACAATGATGCT+CGG + chr1.2:58585948-58585967 None:intergenic 40.0%
TTCCTGATGCAGCAAACATT+AGG + chr1.2:58585678-58585697 None:intergenic 40.0%
! TACCTAATGTTTGCTGCATC+AGG - chr1.2:58585673-58585692 MS.gene066059:intron 40.0%
! TTCCTTGTCTTCTGTTTTCG+GGG + chr1.2:58585407-58585426 None:intergenic 40.0%
!! GTGAGCATTGGGATTAAATG+TGG + chr1.2:58585321-58585340 None:intergenic 40.0%
AAAGGGGAAGGATACCAAAC+AGG + chr1.2:58586500-58586519 None:intergenic 45.0%
AACCACGTTTAGGCATCCTA+CGG + chr1.2:58585541-58585560 None:intergenic 45.0%
AAGGGGAAGGATACCAAACA+GGG + chr1.2:58586499-58586518 None:intergenic 45.0%
ACAAGGAAAGGTCGTGGTAT+TGG - chr1.2:58585419-58585438 MS.gene066059:CDS 45.0%
AGCCAGGAAAGGTTCAAAGT+TGG - chr1.2:58585487-58585506 MS.gene066059:intron 45.0%
ATCTTTGGACCTTCCCTGTT+TGG - chr1.2:58586483-58586502 MS.gene066059:intron 45.0%
CACGAACGTTGAAAGAGTGA+GGG + chr1.2:58585298-58585317 None:intergenic 45.0%
CCAATGGCCAATTCATCTAG+AGG - chr1.2:58586831-58586850 MS.gene066059:intron 45.0%
CCAGGAAAGGTTCAAAGTTG+GGG - chr1.2:58585489-58585508 MS.gene066059:intron 45.0%
CCTCTAGATGAATTGGCCAT+TGG + chr1.2:58586834-58586853 None:intergenic 45.0%
CCTGAGTGGTTTGAGAAGAA+AGG - chr1.2:58587530-58587549 MS.gene066059:CDS 45.0%
CCTTTCTTCTCAAACCACTC+AGG + chr1.2:58587533-58587552 None:intergenic 45.0%
GCCAGGAAAGGTTCAAAGTT+GGG - chr1.2:58585488-58585507 MS.gene066059:intron 45.0%
GGGTTCTTGAAACCACGTTT+AGG + chr1.2:58585551-58585570 None:intergenic 45.0%
GTGGTTCAAGCTCGTATCTT+TGG - chr1.2:58586468-58586487 MS.gene066059:intron 45.0%
TAGTCATAAATCCCTAGCCG+TGG - chr1.2:58586449-58586468 MS.gene066059:intron 45.0%
TCAGCTTCACAATGATGCTC+GGG + chr1.2:58585947-58585966 None:intergenic 45.0%
TTAACCGGGAACTTGACCAT+TGG - chr1.2:58585649-58585668 MS.gene066059:intron 45.0%
TTACCATACACAGGATGCAG+GGG - chr1.2:58586750-58586769 MS.gene066059:intron 45.0%
TTCCCCGAAAACAGAAGACA+AGG - chr1.2:58585402-58585421 MS.gene066059:CDS 45.0%
! AAAGGTCGTGGTATTGGATC+TGG - chr1.2:58585425-58585444 MS.gene066059:CDS 45.0%
! CAAAAGGGAAAAGAGAGTGC+AGG + chr1.2:58587009-58587028 None:intergenic 45.0%
! CGTGGTATTGGATCTGGAAA+GGG - chr1.2:58585431-58585450 MS.gene066059:CDS 45.0%
! CTGCACTCTCTTTTCCCTTT+TGG - chr1.2:58587007-58587026 MS.gene066059:intron 45.0%
! TCGTGGTATTGGATCTGGAA+AGG - chr1.2:58585430-58585449 MS.gene066059:CDS 45.0%
!! ATGGCTGTCACCACTTTTGA+TGG + chr1.2:58587043-58587062 None:intergenic 45.0%
!! CCCATTTAGCCTCACTTTTC+AGG - chr1.2:58585568-58585587 MS.gene066059:intron 45.0%
!! GTGGTTCTGAGAAGATCCAA+TGG - chr1.2:58586815-58586834 MS.gene066059:intron 45.0%
AAAGAGTGAGGGAGCTGCAA+GGG + chr1.2:58585287-58585306 None:intergenic 50.0%
AAAGGGAAAGACTGCTGGGA+GGG - chr1.2:58585448-58585467 MS.gene066059:intron 50.0%
AGGTAACAAGGGTCACTGTC+AGG - chr1.2:58587426-58587445 MS.gene066059:CDS 50.0%
CAGAAGACAAGGAAAGGTCG+TGG - chr1.2:58585413-58585432 MS.gene066059:CDS 50.0%
CCCCAACTTTGAACCTTTCC+TGG + chr1.2:58585492-58585511 None:intergenic 50.0%
CTGGAAAGGGAAAGACTGCT+GGG - chr1.2:58585444-58585463 MS.gene066059:intron 50.0%
GATAGACGGGTATCCGAGAA+CGG + chr1.2:58585255-58585274 None:intergenic 50.0%
GCACGAACGTTGAAAGAGTG+AGG + chr1.2:58585299-58585318 None:intergenic 50.0%
GGAAGGATACCAAACAGGGA+AGG + chr1.2:58586495-58586514 None:intergenic 50.0%
GGAGCTGCAAGGGATGAAAT+TGG + chr1.2:58585277-58585296 None:intergenic 50.0%
GGTAACAAGGGTCACTGTCA+GGG - chr1.2:58587427-58587446 MS.gene066059:CDS 50.0%
GGTTCAAAGTTGGGGTTCGA+AGG - chr1.2:58585497-58585516 MS.gene066059:intron 50.0%
GTAGCCAATGGTCAAGTTCC+CGG + chr1.2:58585656-58585675 None:intergenic 50.0%
TCAAAGTTGGGGTTCGAAGG+AGG - chr1.2:58585500-58585519 MS.gene066059:intron 50.0%
TCTGGAAAGGGAAAGACTGC+TGG - chr1.2:58585443-58585462 MS.gene066059:intron 50.0%
! TTGAACCTTTCCTGGCTCTC+TGG + chr1.2:58585484-58585503 None:intergenic 50.0%
!! AGGATCAAAAGGCATCACCG+TGG + chr1.2:58586903-58586922 None:intergenic 50.0%
AAAGGCCAGAGAGCCAGGAA+AGG - chr1.2:58585476-58585495 MS.gene066059:intron 55.0%
AAGCAGTTGAAGCGGCTGGT+GGG - chr1.2:58587456-58587475 MS.gene066059:CDS 55.0%
AAGGAAGCAGTTGAAGCGGC+TGG - chr1.2:58587452-58587471 MS.gene066059:CDS 55.0%
AAGGGAAAGACTGCTGGGAG+GGG - chr1.2:58585449-58585468 MS.gene066059:intron 55.0%
ACGAGCTTGAACCACGGCTA+GGG + chr1.2:58586463-58586482 None:intergenic 55.0%
AGCCGCTTCAACTGCTTCCT+TGG + chr1.2:58587453-58587472 None:intergenic 55.0%
GAAAGAGTGAGGGAGCTGCA+AGG + chr1.2:58585288-58585307 None:intergenic 55.0%
GAAAGGGAAAGACTGCTGGG+AGG - chr1.2:58585447-58585466 MS.gene066059:intron 55.0%
GCTGACCTACTGGAAGATCC+TGG + chr1.2:58587667-58587686 None:intergenic 55.0%
GCTGAGTGGCTTGACTGTGT+TGG - chr1.2:58586074-58586093 MS.gene066059:CDS 55.0%
GGAAGCCAGGATCTTCCAGT+AGG - chr1.2:58587659-58587678 MS.gene066059:CDS 55.0%
GGAGTAAGATTGATGGGGCG+TGG - chr1.2:58586796-58586815 MS.gene066059:intron 55.0%
GGCCAAGGAAGCAGTTGAAG+CGG - chr1.2:58587448-58587467 MS.gene066059:CDS 55.0%
GTACCCCTGCATCCTGTGTA+TGG + chr1.2:58586756-58586775 None:intergenic 55.0%
TACGAGCTTGAACCACGGCT+AGG + chr1.2:58586464-58586483 None:intergenic 55.0%
TAGGCATCCTACGGCGAAGA+GGG + chr1.2:58585532-58585551 None:intergenic 55.0%
TTAGGCATCCTACGGCGAAG+AGG + chr1.2:58585533-58585552 None:intergenic 55.0%
! GGAATTGGCTTCTTCGGAGC+AGG + chr1.2:58587626-58587645 None:intergenic 55.0%
! GGAGGAGGTCTAGCTGCTTT+AGG + chr1.2:58587566-58587585 None:intergenic 55.0%
!! ACACAGGATGCAGGGGTACT+TGG - chr1.2:58586757-58586776 MS.gene066059:intron 55.0%
!! CACAGGATGCAGGGGTACTT+GGG - chr1.2:58586758-58586777 MS.gene066059:intron 55.0%
AAGGGTCACTGTCAGGGCCA+AGG - chr1.2:58587433-58587452 MS.gene066059:CDS 60.0%
ACTGCTGGGAGGGGTCACAA+AGG - chr1.2:58585458-58585477 MS.gene066059:intron 60.0%
AGGCATCCTACGGCGAAGAG+GGG + chr1.2:58585531-58585550 None:intergenic 60.0%
CGCCGTAGGATGCCTAAACG+TGG - chr1.2:58585536-58585555 MS.gene066059:intron 60.0%
GAAGCAGTTGAAGCGGCTGG+TGG - chr1.2:58587455-58587474 MS.gene066059:CDS 60.0%
GTCACAAAGGCCAGAGAGCC+AGG - chr1.2:58585471-58585490 MS.gene066059:intron 60.0%
TCCTACGGCGAAGAGGGGTT+TGG + chr1.2:58585526-58585545 None:intergenic 60.0%
! GGGCACTGTTGAAGCCTGAG+TGG - chr1.2:58587516-58587535 MS.gene066059:CDS 60.0%
GCCAAACCCCTCTTCGCCGT+AGG - chr1.2:58585522-58585541 MS.gene066059:intron 65.0%
! GGCTGGTGGGTCCGTCAGAA+AGG - chr1.2:58587469-58587488 MS.gene066059:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 58585188 58587694 58585188 ID=MS.gene066059
chr1.2 mRNA 58585188 58587694 58585188 ID=MS.gene066059.t1;Parent=MS.gene066059
chr1.2 exon 58587293 58587694 58587293 ID=MS.gene066059.t1.exon1;Parent=MS.gene066059.t1
chr1.2 CDS 58587293 58587694 58587293 ID=cds.MS.gene066059.t1;Parent=MS.gene066059.t1
chr1.2 exon 58586210 58586293 58586210 ID=MS.gene066059.t1.exon2;Parent=MS.gene066059.t1
chr1.2 CDS 58586210 58586293 58586210 ID=cds.MS.gene066059.t1;Parent=MS.gene066059.t1
chr1.2 exon 58586030 58586119 58586030 ID=MS.gene066059.t1.exon3;Parent=MS.gene066059.t1
chr1.2 CDS 58586030 58586119 58586030 ID=cds.MS.gene066059.t1;Parent=MS.gene066059.t1
chr1.2 exon 58585188 58585454 58585188 ID=MS.gene066059.t1.exon4;Parent=MS.gene066059.t1
chr1.2 CDS 58585188 58585454 58585188 ID=cds.MS.gene066059.t1;Parent=MS.gene066059.t1
Gene Sequence

>MS.gene066059

ATGCTAAGAAGAAGGCTTTCTGTTCTCTCGACTTCAATCATCAGAAGTTCGACTCACAACAAATCCGTTCTCGGATACCCGTCTATCCAATTTCATCCCTTGCAGCTCCCTCACTCTTTCAACGTTCGTGCCACATTTAATCCCAATGCTCACCCAAATTTTCAAGGTTTTAGAGCTTACAGTCTTCTGGCCTTGAATGATCTGAGAGATAATGTTCCCCGAAAACAGAAGACAAGGAAAGGTCGTGGTATTGGATCTGGAAAGGGAAAGACTGCTGGGAGGGGTCACAAAGGCCAGAGAGCCAGGAAAGGTTCAAAGTTGGGGTTCGAAGGAGGCCAAACCCCTCTTCGCCGTAGGATGCCTAAACGTGGTTTCAAGAACCCATTTAGCCTCACTTTTCAGGTATACTTCTTCCTGTTTATATATCTTTTTTGTTTTTGATTAATGATTCTTCTGTGTATTTAACCGGGAACTTGACCATTGGCTACCTAATGTTTGCTGCATCAGGAAAATGAAATTACGCCTTAGTATATTATTAGAGTTCATTAGTTATAATTGTGGAACTCCTATAGTTTTGAATATTATAATTCTGAGATTTATGGGTGGTTACTTGTGAGCAATAATGGAGTTCTCCATCTGAGACTTTTTCAGAATTTCTTTTGCATACTTATTAAAACCAATAAAGACTATTCTGCTTGACTTCCATTCCAAGAAAGAAAACCATTTCTCCAGGAATATTATTAAAAATTATGCATACCCGAGCATCATTGTGAAGCTGAAGTGCCATGCTTAATGCAAATATTAGGTTTTAATTTTATTACAAGACATCATATGCAAGATAAGATTTTTATTTCAAGGACTCTGAAATCTTCTGTAAACATATATGGCTGAGTGGCTTGACTGTGTTGGTTTTGATTGATAAACCTCTTATTTCTACATGTCAATTGCCAAGTATGATATGCACTAAAAAGTAATGTGCATGTCGACAACAAATATATTTCAAAGTTTATACTTTATTGTATTGACATTTGACGGATATTTACCAATGTATTTTAACTGTGTGTGTGTGTGATTTTTGATATAGAATGACATAATGCCAAATGTGTAGTTCACTTTGGCTGATATTTGAGCCTTCAGATTTATGAGTGTCAAGTAACTTCTAAGGGACTATTCTAGTTGTATTAAATAATTTTGTGTGCATAATTTAATTCTTTAATATTTTTTTTATTTTAATGTCTAGATAAGTTTATGTGGAGTAGAGTAGTCATAAATCCCTAGCCGTGGTTCAAGCTCGTATCTTTGGACCTTCCCTGTTTGGTATCCTTCCCCTTTATTCCTCAATCTATCAGTCATATTATCTACTGCTGTATTCAGAAGTTTGTTTTATTTCTTCTTTTACAGCCAATAGGTTTGGGAAAAATTGCAACATTTATCAATGCTGGCAAGATAGATTCTTCTGAATTGATCACAATGAAAACACTCAAGGTTTTTGTGACCGCTTCCTCACTTAAAATGAATTTGTTTGCTTCATGTGCTCCCTCTCATTCTTTTGTTTATTATTATTACCATACACAGGATGCAGGGGTACTTGGGAAGCAAATTAAAGATGGAGTAAGATTGATGGGGCGTGGTTCTGAGAAGATCCAATGGCCAATTCATCTAGAGGTATGTATACTTTGATTTGAAGGCTAAGTAATAAGTGTTTTTGAGGACCACGGTGATGCCTTTTGATCCTTAAATCTTTAGAAATACTTTTTAACTTTTTTGAACTTATCTGATATCATTAATTTTGAGCTTATTTTGATACTGGCTTGAAAGCCTGCACTCTCTTTTCCCTTTTGGCAAGTGTAAACCATCAAAAGTGGTGACAGCCATGTTTTAACATTGGCAACATGAATGGTATATAAAATTTCTGTAGTGTCTAAGGATAAAGAACAAAATGTTGGCTTTAGGTTTCGTAATTTCGATTTTGCTATGAAGAGTTGGAATAGCTCTTATATCTTCAAAGTTTACTTGCTAATTTTCTCAAACTTAGACATTTTCTGTTTTGTATTTCAAATAAAGAATTAAACTCATATAGTATGAGATTTTGATTACATGTTATGCTTCTGTTTGTCAATGAGATAAATAAATGTAAAATTGGTACTTTTTTCAATTCTAAACACTTAAGGTGTAGGTTTTATACAGTCACAAACTTTATAGGCATATAAGAGGGGTGTTATAACTTATTGAGTTTACAGGTAACAAGGGTCACTGTCAGGGCCAAGGAAGCAGTTGAAGCGGCTGGTGGGTCCGTCAGAAAGGTGTATTACAATAAGTTAGGCTTTCGGGCACTGTTGAAGCCTGAGTGGTTTGAGAAGAAAGGAAGATTGTTGCCTAAAGCAGCTAGACCTCCTCCAAAACAAAAGGATAAAGTTGATAGCATTGGACGGCTGCCTGCTCCGAAGAAGCCAATTCCATTTTTAGTTGAAGGAAGCCAGGATCTTCCAGTAGGTCAGCTCAGTTAA
Protein sequence

>MS.gene066059.t1

MLRRRLSVLSTSIIRSSTHNKSVLGYPSIQFHPLQLPHSFNVRATFNPNAHPNFQGFRAYSLLALNDLRDNVPRKQKTRKGRGIGSGKGKTAGRGHKGQRARKGSKLGFEGGQTPLRRRMPKRGFKNPFSLTFQPIGLGKIATFINAGKIDSSELITMKTLKDAGVLGKQIKDGVRLMGRGSEKIQWPIHLEVTRVTVRAKEAVEAAGGSVRKVYYNKLGFRALLKPEWFEKKGRLLPKAARPPPKQKDKVDSIGRLPAPKKPIPFLVEGSQDLPVGQLS