AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043435

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043435.t1 MTR_1g093060 92.517 294 22 0 1 294 54 347 0.0 565
MS.gene043435.t1 MTR_6g006970 91.837 294 24 0 1 294 1 294 0.0 563
MS.gene043435.t1 MTR_2g090370 97.115 104 3 0 189 292 1 104 7.79e-71 219
MS.gene043435.t1 MTR_3g118210 42.029 207 114 6 49 251 58 262 1.92e-43 149
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043435.t1 AT3G17465 64.815 324 84 4 1 294 1 324 2.34e-143 406
MS.gene043435.t1 AT2G43030 39.320 206 121 4 49 251 57 261 2.05e-39 139

Find 86 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGGATGTGGATCCATTTA+TGG 0.158517 2.2:-9047500 MS.gene043435:CDS
TGACTGCACTTTGGGATAAA+TGG 0.171985 2.2:-9049646 MS.gene043435:CDS
CTTTAATCAGTAACCATAAA+TGG 0.218927 2.2:+9047487 None:intergenic
GAGTTCCAATTTCTATCCTT+TGG 0.251026 2.2:-9049616 MS.gene043435:CDS
AGTTCCAATTTCTATCCTTT+GGG 0.317691 2.2:-9049615 MS.gene043435:CDS
CATAGAACCATGGGTTCTTC+AGG 0.322897 2.2:-9048494 MS.gene043435:CDS
CTCCTGAAGCTTCCTCTTCA+TGG 0.339965 2.2:+9049318 None:intergenic
TCAACCCAAAGGATAGAAAT+TGG 0.341302 2.2:+9049611 None:intergenic
ACCCACATCAAATTTCTTGC+TGG 0.343726 2.2:+9048210 None:intergenic
TCATCTGTATCTTCATCTTC+TGG 0.348247 2.2:+9047547 None:intergenic
AAACCTCTGGTTGCTGATCT+TGG 0.349915 2.2:-9047520 MS.gene043435:CDS
CATACTGTCCGGGAACAAAA+TGG 0.364379 2.2:+9049242 None:intergenic
AACCTCTGGTTGCTGATCTT+GGG 0.368580 2.2:-9047519 MS.gene043435:CDS
ACACCAGTTCGTTGTGAATT+CGG 0.370596 2.2:+9049689 None:intergenic
GCCCTAAAATGACCCACTTC+AGG 0.379527 2.2:+9049352 None:intergenic
GATTGGTTGTGGACAGAAGA+AGG 0.380825 2.2:-9049392 MS.gene043435:CDS
CTTCTGTTTACAGGGTGTAA+TGG 0.384171 2.2:-9048567 MS.gene043435:intron
AGGAATGCTTGGTCCTTGTA+AGG 0.397571 2.2:-9048474 MS.gene043435:intron
TCCCATGAAGAGGAAGCTTC+AGG 0.402123 2.2:-9049320 MS.gene043435:CDS
ATGTGGAATGACTGCACTTT+GGG 0.405645 2.2:-9049654 MS.gene043435:CDS
GACCAATAGCCTTCCTTCTC+AGG 0.413508 2.2:+9049560 None:intergenic
ATGCAGGTCCCAGGAGCTAC+AGG 0.423543 2.2:-9047646 MS.gene043435:intron
GACTGCACTTTGGGATAAAT+GGG 0.426337 2.2:-9049645 MS.gene043435:CDS
ACAAAGTTCCCTGTAGCTCC+TGG 0.431609 2.2:+9047637 None:intergenic
AACCGCGGCAGTGGCACCAC+AGG 0.433121 2.2:+9049770 None:intergenic
TCTTCTGGCACAAAGTAAGT+TGG 0.439664 2.2:+9047562 None:intergenic
GTTCGGAGGTGGAGACTATA+CGG 0.442293 2.2:-9049742 MS.gene043435:CDS
GGTTGCTGATCTTGGGGATG+TGG 0.454818 2.2:-9047512 MS.gene043435:CDS
AATGGACCGGTATGATTTCA+AGG 0.462175 2.2:-9048549 MS.gene043435:CDS
TGATGACAACATTGTCTCTC+AGG 0.468175 2.2:-9049591 MS.gene043435:CDS
AGAAATTTGATGTGGGTGAA+AGG 0.489286 2.2:-9048204 MS.gene043435:CDS
CTTCTGGCACAAAGTAAGTT+GGG 0.491443 2.2:+9047563 None:intergenic
ACCTCTGGTTGCTGATCTTG+GGG 0.494514 2.2:-9047518 MS.gene043435:CDS
ACACCGAATTCACAACGAAC+TGG 0.499424 2.2:-9049692 MS.gene043435:CDS
ACAGGAATCACTAAAGGAAA+AGG 0.499985 2.2:-9049211 MS.gene043435:CDS
GGACTCCATCCAACCTTACA+AGG 0.504118 2.2:+9048461 None:intergenic
ATGGGTTCTTCAGGAATGCT+TGG 0.507345 2.2:-9048485 MS.gene043435:CDS
ACATTAAGCGATGTACCAAC+AGG 0.508189 2.2:+9049268 None:intergenic
GAGGCGTGGTTTCTCGTCTA+CGG 0.509855 2.2:-9049799 MS.gene043435:CDS
TGTGATGTCAACATACTGTC+CGG 0.509894 2.2:+9049231 None:intergenic
TATGTGCTTTCGAATTCCCA+TGG 0.513084 2.2:+9048513 None:intergenic
CTCCTGAGAAGGAAGGCTAT+TGG 0.515568 2.2:-9049562 MS.gene043435:CDS
TCCTGAAGCTTCCTCTTCAT+GGG 0.524646 2.2:+9049319 None:intergenic
TGCTTCCCACCCATTCTCCC+CGG 0.526048 2.2:+9048270 None:intergenic
TTTGATGTGGGTGAAAGGCC+AGG 0.528326 2.2:-9048199 MS.gene043435:intron
GGAAGGCTATTGGTCTCTCC+AGG 0.530451 2.2:-9049552 MS.gene043435:intron
AAAATGCCGGGGAGAATGGG+TGG 0.532086 2.2:-9048276 MS.gene043435:CDS
AATGTGGAATGACTGCACTT+TGG 0.543439 2.2:-9049655 MS.gene043435:CDS
AAAGGAAGTATAGATGTCTC+AGG 0.543480 2.2:+9047586 None:intergenic
TACAGATGATATGAAACCTC+TGG 0.545460 2.2:-9047533 MS.gene043435:CDS
GACATCACAGGAATCACTAA+AGG 0.546953 2.2:-9049217 MS.gene043435:CDS
GAGGATGCACTTCTTCCTGT+TGG 0.552055 2.2:-9049283 MS.gene043435:CDS
CTCAAGGAGTTCCCATGAAG+AGG 0.552464 2.2:-9049330 MS.gene043435:CDS
GTTTACAGGGTGTAATGGAC+CGG 0.553779 2.2:-9048562 MS.gene043435:intron
CAAAGTTCCCTGTAGCTCCT+GGG 0.553971 2.2:+9047638 None:intergenic
GAATTCGGTGTCATCACACT+AGG 0.557489 2.2:+9049704 None:intergenic
AAGGGAGGTTCAGCATCCCA+TGG 0.557581 2.2:-9048530 MS.gene043435:CDS
ATGTGCACTATGCAGGTCCC+AGG 0.560952 2.2:-9047655 MS.gene043435:intron
TCCCTGTGGTGCCACTGCCG+CGG 0.562920 2.2:-9049772 MS.gene043435:CDS
TCAGGTCAAAACTCCTGAGA+AGG 0.567631 2.2:-9049573 MS.gene043435:CDS
ATGTGCTTTCGAATTCCCAT+GGG 0.571228 2.2:+9048514 None:intergenic
ACACTCGACAAAGCCTGAAG+TGG 0.575978 2.2:-9049365 MS.gene043435:CDS
CACTCGACAAAGCCTGAAGT+GGG 0.584112 2.2:-9049364 MS.gene043435:CDS
AACCTCCCTTGAAATCATAC+CGG 0.585348 2.2:+9048543 None:intergenic
TCAGGAGTTTCGAGTGACAG+AGG 0.587986 2.2:-9049302 MS.gene043435:CDS
GTGATGTCAACATACTGTCC+GGG 0.588163 2.2:+9049232 None:intergenic
GGCACAAAGTAAGTTGGGAA+AGG 0.599353 2.2:+9047568 None:intergenic
GACCGGTATGATTTCAAGGG+AGG 0.601360 2.2:-9048545 MS.gene043435:CDS
TCGAAAGCACATAGAACCAT+GGG 0.605881 2.2:-9048503 MS.gene043435:CDS
GGACAGTATGTTGACATCAC+AGG 0.614169 2.2:-9049229 MS.gene043435:CDS
AAATGCCGGGGAGAATGGGT+GGG 0.614539 2.2:-9048275 MS.gene043435:CDS
AGGGAGGTTCAGCATCCCAT+GGG 0.616692 2.2:-9048529 MS.gene043435:CDS
GTCAAAACTCCTGAGAAGGA+AGG 0.621009 2.2:-9049569 MS.gene043435:CDS
ATCCAGCAAGAAATTTGATG+TGG 0.622717 2.2:-9048212 MS.gene043435:CDS
TTCGAAAGCACATAGAACCA+TGG 0.623227 2.2:-9048504 MS.gene043435:CDS
AAGCATTCCTGAAGAACCCA+TGG 0.626684 2.2:+9048487 None:intergenic
ACCGCGGCAGTGGCACCACA+GGG 0.627605 2.2:+9049771 None:intergenic
TGCAGGTCCCAGGAGCTACA+GGG 0.631734 2.2:-9047645 MS.gene043435:intron
ATGGACCGGTATGATTTCAA+GGG 0.635642 2.2:-9048548 MS.gene043435:CDS
GGTGTTATAGCTGTAAAATG+TGG 0.647527 2.2:-9049671 MS.gene043435:CDS
CAATGTTGTCATCAACCCAA+AGG 0.648583 2.2:+9049600 None:intergenic
TCGTCTACGGCAATTCCCTG+TGG 0.657376 2.2:-9049786 MS.gene043435:CDS
TCCAGCAAGAAATTTGATGT+GGG 0.677547 2.2:-9048211 MS.gene043435:CDS
TCCCCAAGATCAGCAACCAG+AGG 0.694021 2.2:+9047517 None:intergenic
CGGAGGTGGAGACTATACGG+AGG 0.753244 2.2:-9049739 MS.gene043435:CDS
ACTGCACTTTGGGATAAATG+GGG 0.757943 2.2:-9049644 MS.gene043435:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAAAATAGATACAAAA+TGG + chr2.2:9049547-9049566 None:intergenic 10.0%
!! AAATATATTATCACAACTAT+AGG + chr2.2:9048978-9048997 None:intergenic 15.0%
!! AAGAAAAAATACTAATCTAA+AGG + chr2.2:9048867-9048886 None:intergenic 15.0%
!! GTTTATAATGAATTATTAAG+CGG - chr2.2:9047810-9047829 MS.gene043435:intron 15.0%
!! AAAATATCAACTAAGATAGT+TGG - chr2.2:9049379-9049398 MS.gene043435:CDS 20.0%
!! AAAGAACAGTAAAAAATGTA+TGG - chr2.2:9049056-9049075 MS.gene043435:intron 20.0%
!! AAATCGTATATAAAATTACC+TGG + chr2.2:9049126-9049145 None:intergenic 20.0%
!! AAGAACAGTAAAAAATGTAT+GGG - chr2.2:9049057-9049076 MS.gene043435:intron 20.0%
!! ACAATTGAACAAATAAATAG+TGG + chr2.2:9048386-9048405 None:intergenic 20.0%
!! AGGTAAATAAAATTTGAATG+AGG + chr2.2:9049299-9049318 None:intergenic 20.0%
!! ATTTATAAAATTGCAGCTAT+AGG - chr2.2:9049142-9049161 MS.gene043435:intron 20.0%
!! GAACTTAAAAATATAAACGA+GGG + chr2.2:9047847-9047866 None:intergenic 20.0%
!! GCCAATTATTTATTAATAAC+TGG - chr2.2:9049567-9049586 MS.gene043435:CDS 20.0%
!! GCCAGTTATTAATAAATAAT+TGG + chr2.2:9049571-9049590 None:intergenic 20.0%
!! TGAACTTAAAAATATAAACG+AGG + chr2.2:9047848-9047867 None:intergenic 20.0%
!! TGTCAAAGAATTACATAATT+GGG - chr2.2:9048293-9048312 MS.gene043435:CDS 20.0%
!! TTGTCAAAGAATTACATAAT+TGG - chr2.2:9048292-9048311 MS.gene043435:CDS 20.0%
!!! ACATGTTTGATATTTTGTTT+TGG - chr2.2:9047886-9047905 MS.gene043435:intron 20.0%
!!! ATATTTTGTTTTGGTTAGAT+TGG - chr2.2:9047895-9047914 MS.gene043435:intron 20.0%
!!! ATTCAAATTTTATTTACCTC+TGG - chr2.2:9049300-9049319 MS.gene043435:CDS 20.0%
!!! CTATCTTAGTTGATATTTTT+TGG + chr2.2:9049378-9049397 None:intergenic 20.0%
!!! TTTCCGATTTATTTACTTTT+GGG - chr2.2:9048410-9048429 MS.gene043435:intron 20.0%
!!! TTTTAAAGGTAAAAAAATGC+CGG - chr2.2:9049015-9049034 MS.gene043435:intron 20.0%
! CAAATTATCTTCTGTTTACA+GGG - chr2.2:9048729-9048748 MS.gene043435:intron 25.0%
! TAATAACTGGCCAAAATATT+TGG - chr2.2:9049580-9049599 MS.gene043435:CDS 25.0%
! TCAAATTATCTTCTGTTTAC+AGG - chr2.2:9048728-9048747 MS.gene043435:intron 25.0%
! TTATAATCCATGTATGACTT+CGG - chr2.2:9048600-9048619 MS.gene043435:intron 25.0%
! TTTAAAGGTAAAAAAATGCC+GGG - chr2.2:9049016-9049035 MS.gene043435:intron 25.0%
!! GTTTCCGATTTATTTACTTT+TGG - chr2.2:9048409-9048428 MS.gene043435:intron 25.0%
AGGACATAGATCTGTAAAAA+TGG + chr2.2:9048687-9048706 None:intergenic 30.0%
AGTATTATATAGACACAAGC+AGG + chr2.2:9048242-9048261 None:intergenic 30.0%
AGTTCCAATTTCTATCCTTT+GGG - chr2.2:9047689-9047708 MS.gene043435:intron 30.0%
ATTTGAATGAGGATTGTTTC+AGG + chr2.2:9049288-9049307 None:intergenic 30.0%
CGATATCATTAAGAAAACGA+TGG - chr2.2:9049510-9049529 MS.gene043435:intron 30.0%
TACTGCAATGTGAATTAAGT+GGG + chr2.2:9049439-9049458 None:intergenic 30.0%
TCGCCCAAAAGTAAATAAAT+CGG + chr2.2:9048416-9048435 None:intergenic 30.0%
TTAAAGGTAAAAAAATGCCG+GGG - chr2.2:9049017-9049036 MS.gene043435:intron 30.0%
TTAACAGTCATGTAACTGAA+AGG + chr2.2:9048707-9048726 None:intergenic 30.0%
! AAGTATCCGAGTTTAAAACA+TGG - chr2.2:9049255-9049274 MS.gene043435:CDS 30.0%
! AATGAGAAGTATGTAGTATG+AGG + chr2.2:9048141-9048160 None:intergenic 30.0%
! AGCCAACATTCCAAATATTT+TGG + chr2.2:9049593-9049612 None:intergenic 30.0%
! ATGAGAAGTATGTAGTATGA+GGG + chr2.2:9048140-9048159 None:intergenic 30.0%
! CATTTTGTCGTTCAAATTTC+AGG + chr2.2:9048520-9048539 None:intergenic 30.0%
! TATATTTTTCCTCACTGTTC+AGG - chr2.2:9048991-9049010 MS.gene043435:intron 30.0%
! TTGATTTTCATGCTTTCTAG+GGG - chr2.2:9048540-9048559 MS.gene043435:CDS 30.0%
!! GTTGATTTTCATGCTTTCTA+GGG - chr2.2:9048539-9048558 MS.gene043435:CDS 30.0%
!! TTTGAGCCATGTTTTAAACT+CGG + chr2.2:9049264-9049283 None:intergenic 30.0%
!!! ATTTTTTGGTCTTAAACCTC+CGG + chr2.2:9049364-9049383 None:intergenic 30.0%
!!! TGTTGATTTTCATGCTTTCT+AGG - chr2.2:9048538-9048557 MS.gene043435:CDS 30.0%
AAACCTTCCATCGAGTAAAT+CGG - chr2.2:9048644-9048663 MS.gene043435:intron 35.0%
AATTGGTCGGCAACAATAAT+AGG - chr2.2:9048341-9048360 MS.gene043435:intron 35.0%
ACAGGAATCACTAAAGGAAA+AGG - chr2.2:9048093-9048112 MS.gene043435:intron 35.0%
AGAAATTTGATGTGGGTGAA+AGG - chr2.2:9049100-9049119 MS.gene043435:intron 35.0%
ATCCAGCAAGAAATTTGATG+TGG - chr2.2:9049092-9049111 MS.gene043435:intron 35.0%
ATTTACTCGATGGAAGGTTT+CGG + chr2.2:9048644-9048663 None:intergenic 35.0%
ATTTGATCGAGAGAACGAAA+TGG + chr2.2:9047784-9047803 None:intergenic 35.0%
GAGTTCCAATTTCTATCCTT+TGG - chr2.2:9047688-9047707 MS.gene043435:intron 35.0%
GGGAGGAGATAAAAGAATAA+CGG + chr2.2:9048481-9048500 None:intergenic 35.0%
GTACTGCAATGTGAATTAAG+TGG + chr2.2:9049440-9049459 None:intergenic 35.0%
TAAAATTGCAGCTATAGGAC+AGG - chr2.2:9049147-9049166 MS.gene043435:intron 35.0%
TACAGATGATATGAAACCTC+TGG - chr2.2:9049771-9049790 MS.gene043435:CDS 35.0%
TATTATTGTTGCCGACCAAT+TGG + chr2.2:9048342-9048361 None:intergenic 35.0%
TCAACCCAAAGGATAGAAAT+TGG + chr2.2:9047696-9047715 None:intergenic 35.0%
TCATCTGTATCTTCATCTTC+TGG + chr2.2:9049760-9049779 None:intergenic 35.0%
TCCAGCAAGAAATTTGATGT+GGG - chr2.2:9049093-9049112 MS.gene043435:intron 35.0%
TTCAGTAGTTTCAACTGATC+AGG - chr2.2:9048445-9048464 MS.gene043435:intron 35.0%
TTGTCGTTCAAATTTCAGGA+GGG + chr2.2:9048516-9048535 None:intergenic 35.0%
TTTGTCGTTCAAATTTCAGG+AGG + chr2.2:9048517-9048536 None:intergenic 35.0%
! AAAAACTGATTCAACTCGAG+TGG - chr2.2:9049199-9049218 MS.gene043435:intron 35.0%
! AAAGGAAGTATAGATGTCTC+AGG + chr2.2:9049721-9049740 None:intergenic 35.0%
! CACTAAAGGAAAAGGTTTTC+AGG - chr2.2:9048101-9048120 MS.gene043435:intron 35.0%
! CTTTAAAAACCTGAACAGTG+AGG + chr2.2:9049003-9049022 None:intergenic 35.0%
! GGCCAAAATATTTGGAATGT+TGG - chr2.2:9049588-9049607 MS.gene043435:CDS 35.0%
! GGTGTTATAGCTGTAAAATG+TGG - chr2.2:9047633-9047652 MS.gene043435:CDS 35.0%
! TGATTTTCATGCTTTCTAGG+GGG - chr2.2:9048541-9048560 MS.gene043435:CDS 35.0%
!! CTCACTGTTCAGGTTTTTAA+AGG - chr2.2:9049001-9049020 MS.gene043435:intron 35.0%
!! TTTGGAATGTTGGCTTTATG+CGG - chr2.2:9049598-9049617 MS.gene043435:CDS 35.0%
!!! AGTTTTTTTTTTTCCCCGAG+GGG + chr2.2:9049186-9049205 None:intergenic 35.0%
!!! CAGTTTTTTTTTTTCCCCGA+GGG + chr2.2:9049187-9049206 None:intergenic 35.0%
!!! TCAGTTTTTTTTTTTCCCCG+AGG + chr2.2:9049188-9049207 None:intergenic 35.0%
!!! TGTTTTGGTTAGATTGGTTG+TGG - chr2.2:9047901-9047920 MS.gene043435:intron 35.0%
AAAAAAATGCCGGGGAGAAT+GGG - chr2.2:9049025-9049044 MS.gene043435:intron 40.0%
AACCTCCCTTGAAATCATAC+CGG + chr2.2:9048764-9048783 None:intergenic 40.0%
AACCTTCCATCGAGTAAATC+GGG - chr2.2:9048645-9048664 MS.gene043435:intron 40.0%
AATGGACCGGTATGATTTCA+AGG - chr2.2:9048755-9048774 MS.gene043435:intron 40.0%
AATGTGGAATGACTGCACTT+TGG - chr2.2:9047649-9047668 MS.gene043435:intron 40.0%
ACACCAGTTCGTTGTGAATT+CGG + chr2.2:9047618-9047637 None:intergenic 40.0%
ACATTAAGCGATGTACCAAC+AGG + chr2.2:9048039-9048058 None:intergenic 40.0%
ACCCACATCAAATTTCTTGC+TGG + chr2.2:9049097-9049116 None:intergenic 40.0%
ATGGACCGGTATGATTTCAA+GGG - chr2.2:9048756-9048775 MS.gene043435:intron 40.0%
ATGTGCTTTCGAATTCCCAT+GGG + chr2.2:9048793-9048812 None:intergenic 40.0%
ATGTGGAATGACTGCACTTT+GGG - chr2.2:9047650-9047669 MS.gene043435:intron 40.0%
CAATGTTGTCATCAACCCAA+AGG + chr2.2:9047707-9047726 None:intergenic 40.0%
CTTCTGGCACAAAGTAAGTT+GGG + chr2.2:9049744-9049763 None:intergenic 40.0%
CTTCTGTTTACAGGGTGTAA+TGG - chr2.2:9048737-9048756 MS.gene043435:intron 40.0%
GACATCACAGGAATCACTAA+AGG - chr2.2:9048087-9048106 MS.gene043435:intron 40.0%
GGCTGAACTGTAGATTACTA+GGG - chr2.2:9049321-9049340 MS.gene043435:CDS 40.0%
TAAAAAAATGCCGGGGAGAA+TGG - chr2.2:9049024-9049043 MS.gene043435:intron 40.0%
TAATTGCCCGATTTACTCGA+TGG + chr2.2:9048654-9048673 None:intergenic 40.0%
TATGTGCTTTCGAATTCCCA+TGG + chr2.2:9048794-9048813 None:intergenic 40.0%
TCAGGAGGGGTCAAATAAAA+CGG + chr2.2:9048502-9048521 None:intergenic 40.0%
TCTTCTGGCACAAAGTAAGT+TGG + chr2.2:9049745-9049764 None:intergenic 40.0%
TGATGACAACATTGTCTCTC+AGG - chr2.2:9047713-9047732 MS.gene043435:intron 40.0%
TGGCTGAACTGTAGATTACT+AGG - chr2.2:9049320-9049339 MS.gene043435:CDS 40.0%
TGTCGTTCAAATTTCAGGAG+GGG + chr2.2:9048515-9048534 None:intergenic 40.0%
TGTGATGTCAACATACTGTC+CGG + chr2.2:9048076-9048095 None:intergenic 40.0%
TTGTATGATGTGCACTATGC+AGG - chr2.2:9049642-9049661 MS.gene043435:CDS 40.0%
! AACTATTTTGTATCCCCTCG+GGG - chr2.2:9049170-9049189 MS.gene043435:intron 40.0%
! GAACTATTTTGTATCCCCTC+GGG - chr2.2:9049169-9049188 MS.gene043435:intron 40.0%
! GAAGTATCCGAAGTCATACA+TGG + chr2.2:9048610-9048629 None:intergenic 40.0%
! GGAACTATTTTGTATCCCCT+CGG - chr2.2:9049168-9049187 MS.gene043435:intron 40.0%
! TCGAAAGCACATAGAACCAT+GGG - chr2.2:9048801-9048820 MS.gene043435:intron 40.0%
! TTCGAAAGCACATAGAACCA+TGG - chr2.2:9048800-9048819 MS.gene043435:intron 40.0%
!! AATGTTCGCCATTTTGTTCC+CGG - chr2.2:9048054-9048073 MS.gene043435:intron 40.0%
!! ACTGCACTTTGGGATAAATG+GGG - chr2.2:9047660-9047679 MS.gene043435:intron 40.0%
!! GACTGCACTTTGGGATAAAT+GGG - chr2.2:9047659-9047678 MS.gene043435:intron 40.0%
!! TGACTGCACTTTGGGATAAA+TGG - chr2.2:9047658-9047677 MS.gene043435:intron 40.0%
!! TTGGAATGTTGGCTTTATGC+GGG - chr2.2:9049599-9049618 MS.gene043435:CDS 40.0%
AAACCTCTGGTTGCTGATCT+TGG - chr2.2:9049784-9049803 MS.gene043435:CDS 45.0%
AACCTCTGGTTGCTGATCTT+GGG - chr2.2:9049785-9049804 MS.gene043435:CDS 45.0%
AAGCATTCCTGAAGAACCCA+TGG + chr2.2:9048820-9048839 None:intergenic 45.0%
ACACCGAATTCACAACGAAC+TGG - chr2.2:9047612-9047631 MS.gene043435:CDS 45.0%
AGGAATGCTTGGTCCTTGTA+AGG - chr2.2:9048830-9048849 MS.gene043435:intron 45.0%
ATGCTTGGTCCTTGTAAGGT+TGG - chr2.2:9048834-9048853 MS.gene043435:intron 45.0%
ATGGGTTCTTCAGGAATGCT+TGG - chr2.2:9048819-9048838 MS.gene043435:intron 45.0%
CAGGAGGGGTCAAATAAAAC+GGG + chr2.2:9048501-9048520 None:intergenic 45.0%
CATACTGTCCGGGAACAAAA+TGG + chr2.2:9048065-9048084 None:intergenic 45.0%
CATAGAACCATGGGTTCTTC+AGG - chr2.2:9048810-9048829 MS.gene043435:intron 45.0%
CGTTTCGATCCACATCCAAT+TGG - chr2.2:9048324-9048343 MS.gene043435:intron 45.0%
GGACAGTATGTTGACATCAC+AGG - chr2.2:9048075-9048094 MS.gene043435:intron 45.0%
GTAATCTACAGTTCAGCCAG+AGG + chr2.2:9049319-9049338 None:intergenic 45.0%
GTCAAAACTCCTGAGAAGGA+AGG - chr2.2:9047735-9047754 MS.gene043435:intron 45.0%
GTGATGTCAACATACTGTCC+GGG + chr2.2:9048075-9048094 None:intergenic 45.0%
TCAGGTCAAAACTCCTGAGA+AGG - chr2.2:9047731-9047750 MS.gene043435:intron 45.0%
TCCTGAAGCTTCCTCTTCAT+GGG + chr2.2:9047988-9048007 None:intergenic 45.0%
TCGATCCACATCCAATTGGT+CGG - chr2.2:9048328-9048347 MS.gene043435:intron 45.0%
TGGGGATGTGGATCCATTTA+TGG - chr2.2:9049804-9049823 MS.gene043435:CDS 45.0%
TTGGTCCTTGTAAGGTTGGA+TGG - chr2.2:9048838-9048857 MS.gene043435:intron 45.0%
! GATTGGTTGTGGACAGAAGA+AGG - chr2.2:9047912-9047931 MS.gene043435:intron 45.0%
! GGCACAAAGTAAGTTGGGAA+AGG + chr2.2:9049739-9049758 None:intergenic 45.0%
!! AGCCTGAAGTGGGTCATTTT+AGG - chr2.2:9047950-9047969 MS.gene043435:intron 45.0%
!! GAATTCGGTGTCATCACACT+AGG + chr2.2:9047603-9047622 None:intergenic 45.0%
!! GCCTGAAGTGGGTCATTTTA+GGG - chr2.2:9047951-9047970 MS.gene043435:intron 45.0%
!! GTTTACAGGGTGTAATGGAC+CGG - chr2.2:9048742-9048761 MS.gene043435:intron 45.0%
!! ATAAAAAATTAAAATCATTA+TGG + chr2.2:9049405-9049424 None:intergenic 5.0%
!! TATTATTTATAAAGAAAAAA+AGG + chr2.2:9048164-9048183 None:intergenic 5.0%
ACAAAGTTCCCTGTAGCTCC+TGG + chr2.2:9049670-9049689 None:intergenic 50.0%
ACACTCGACAAAGCCTGAAG+TGG - chr2.2:9047939-9047958 MS.gene043435:intron 50.0%
ACCTCCGAACTCAAAAACCG+CGG + chr2.2:9047552-9047571 None:intergenic 50.0%
ACCTCTGGTTGCTGATCTTG+GGG - chr2.2:9049786-9049805 MS.gene043435:CDS 50.0%
AGAACGAAATGGAACGCACC+TGG + chr2.2:9047773-9047792 None:intergenic 50.0%
CAAAGTTCCCTGTAGCTCCT+GGG + chr2.2:9049669-9049688 None:intergenic 50.0%
CACTCGACAAAGCCTGAAGT+GGG - chr2.2:9047940-9047959 MS.gene043435:intron 50.0%
CTCAAGGAGTTCCCATGAAG+AGG - chr2.2:9047974-9047993 MS.gene043435:intron 50.0%
CTCCTGAAGCTTCCTCTTCA+TGG + chr2.2:9047989-9048008 None:intergenic 50.0%
CTCCTGAGAAGGAAGGCTAT+TGG - chr2.2:9047742-9047761 MS.gene043435:intron 50.0%
GACCAATAGCCTTCCTTCTC+AGG + chr2.2:9047747-9047766 None:intergenic 50.0%
GACCGGTATGATTTCAAGGG+AGG - chr2.2:9048759-9048778 MS.gene043435:intron 50.0%
GAGGATGCACTTCTTCCTGT+TGG - chr2.2:9048021-9048040 MS.gene043435:intron 50.0%
GAGGGGTCAAATAAAACGGG+AGG + chr2.2:9048498-9048517 None:intergenic 50.0%
GCCCTAAAATGACCCACTTC+AGG + chr2.2:9047955-9047974 None:intergenic 50.0%
GGACTCCATCCAACCTTACA+AGG + chr2.2:9048846-9048865 None:intergenic 50.0%
GTTCGGAGGTGGAGACTATA+CGG - chr2.2:9047562-9047581 MS.gene043435:CDS 50.0%
TACTAGGGTTCCTTCCCTCA+CGG - chr2.2:9049336-9049355 MS.gene043435:CDS 50.0%
TCCCATGAAGAGGAAGCTTC+AGG - chr2.2:9047984-9048003 MS.gene043435:intron 50.0%
TGCCCGATTTACTCGATGGA+AGG + chr2.2:9048650-9048669 None:intergenic 50.0%
TGTTGCCGACCAATTGGATG+TGG + chr2.2:9048336-9048355 None:intergenic 50.0%
TTAAACCTCCGGTTCCGTGA+GGG + chr2.2:9049353-9049372 None:intergenic 50.0%
TTTGATGTGGGTGAAAGGCC+AGG - chr2.2:9049105-9049124 MS.gene043435:intron 50.0%
! TCAGGAGTTTCGAGTGACAG+AGG - chr2.2:9048002-9048021 MS.gene043435:intron 50.0%
!!! ACTGCCGCGGTTTTTGAGTT+CGG - chr2.2:9047545-9047564 MS.gene043435:CDS 50.0%
!!! GTGGGTCATTTTAGGGCTCA+AGG - chr2.2:9047958-9047977 MS.gene043435:intron 50.0%
AAAATGCCGGGGAGAATGGG+TGG - chr2.2:9049028-9049047 MS.gene043435:intron 55.0%
AAATGCCGGGGAGAATGGGT+GGG - chr2.2:9049029-9049048 MS.gene043435:intron 55.0%
AAGGGAGGTTCAGCATCCCA+TGG - chr2.2:9048774-9048793 MS.gene043435:intron 55.0%
AGGGAGGTTCAGCATCCCAT+GGG - chr2.2:9048775-9048794 MS.gene043435:intron 55.0%
ATGTGCACTATGCAGGTCCC+AGG - chr2.2:9049649-9049668 MS.gene043435:CDS 55.0%
CTTAAACCTCCGGTTCCGTG+AGG + chr2.2:9049354-9049373 None:intergenic 55.0%
GAACTCAAAAACCGCGGCAG+TGG + chr2.2:9047546-9047565 None:intergenic 55.0%
GAGGCGTGGTTTCTCGTCTA+CGG - chr2.2:9047505-9047524 MS.gene043435:CDS 55.0%
GGTTGCTGATCTTGGGGATG+TGG - chr2.2:9049792-9049811 MS.gene043435:CDS 55.0%
TCCCCAAGATCAGCAACCAG+AGG + chr2.2:9049790-9049809 None:intergenic 55.0%
TCGTCTACGGCAATTCCCTG+TGG - chr2.2:9047518-9047537 MS.gene043435:CDS 55.0%
! GGAAGGCTATTGGTCTCTCC+AGG - chr2.2:9047752-9047771 MS.gene043435:intron 55.0%
!!! GCGGTTTTTGAGTTCGGAGG+TGG - chr2.2:9047551-9047570 MS.gene043435:CDS 55.0%
ATGCAGGTCCCAGGAGCTAC+AGG - chr2.2:9049658-9049677 MS.gene043435:CDS 60.0%
CGGAGGTGGAGACTATACGG+AGG - chr2.2:9047565-9047584 MS.gene043435:CDS 60.0%
GGTTCCTTCCCTCACGGAAC+CGG - chr2.2:9049342-9049361 MS.gene043435:CDS 60.0%
TGCAGGTCCCAGGAGCTACA+GGG - chr2.2:9049659-9049678 MS.gene043435:CDS 60.0%
TGCTTCCCACCCATTCTCCC+CGG + chr2.2:9049037-9049056 None:intergenic 60.0%
!! GCTAGCGTTTTCGAGAGGCG+TGG - chr2.2:9047491-9047510 MS.gene043435:CDS 60.0%
!!! GCCGCGGTTTTTGAGTTCGG+AGG - chr2.2:9047548-9047567 MS.gene043435:CDS 60.0%
ACCTCCGGTTCCGTGAGGGA+AGG + chr2.2:9049349-9049368 None:intergenic 65.0%
TCCTTCCCTCACGGAACCGG+AGG - chr2.2:9049345-9049364 MS.gene043435:CDS 65.0%
AACCGCGGCAGTGGCACCAC+AGG + chr2.2:9047537-9047556 None:intergenic 70.0%
ACCGCGGCAGTGGCACCACA+GGG + chr2.2:9047536-9047555 None:intergenic 70.0%
!! TCCCTGTGGTGCCACTGCCG+CGG - chr2.2:9047532-9047551 MS.gene043435:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 9047489 9049837 9047489 ID=MS.gene043435
chr2.2 mRNA 9047489 9049837 9047489 ID=MS.gene043435.t1;Parent=MS.gene043435
chr2.2 exon 9049553 9049837 9049553 ID=MS.gene043435.t1.exon1;Parent=MS.gene043435.t1
chr2.2 CDS 9049553 9049837 9049553 ID=cds.MS.gene043435.t1;Parent=MS.gene043435.t1
chr2.2 exon 9049204 9049413 9049204 ID=MS.gene043435.t1.exon2;Parent=MS.gene043435.t1
chr2.2 CDS 9049204 9049413 9049204 ID=cds.MS.gene043435.t1;Parent=MS.gene043435.t1
chr2.2 exon 9048475 9048576 9048475 ID=MS.gene043435.t1.exon3;Parent=MS.gene043435.t1
chr2.2 CDS 9048475 9048576 9048475 ID=cds.MS.gene043435.t1;Parent=MS.gene043435.t1
chr2.2 exon 9048200 9048313 9048200 ID=MS.gene043435.t1.exon4;Parent=MS.gene043435.t1
chr2.2 CDS 9048200 9048313 9048200 ID=cds.MS.gene043435.t1;Parent=MS.gene043435.t1
chr2.2 exon 9047489 9047662 9047489 ID=MS.gene043435.t1.exon5;Parent=MS.gene043435.t1
chr2.2 CDS 9047489 9047662 9047489 ID=cds.MS.gene043435.t1;Parent=MS.gene043435.t1
Gene Sequence

>MS.gene043435

ATGCTAGCGTTTTCGAGAGGCGTGGTTTCTCGTCTACGGCAATTCCCTGTGGTGCCACTGCCGCGGTTTTTGAGTTCGGAGGTGGAGACTATACGGAGGATTGAAGCAAAACCTAGTGTGATGACACCGAATTCACAACGAACTGGTGTTATAGCTGTAAAATGTGGAATGACTGCACTTTGGGATAAATGGGGTGCTAGAGTTCCAATTTCTATCCTTTGGGTTGATGACAACATTGTCTCTCAGGTCAAAACTCCTGAGAAGGAAGGCTATTGGTCTCTCCAGGTGCGTTCCATTTCGTTCTCTCGATCAAATTTTAATGTTTATAATGAATTATTAAGCGGAGTTGCTTGTTCCCTCGTTTATATTTTTAAGTTCAATGTGTATCTAATTATAGACATGTTTGATATTTTGTTTTGGTTAGATTGGTTGTGGACAGAAGAAGGAGAAACACTCGACAAAGCCTGAAGTGGGTCATTTTAGGGCTCAAGGAGTTCCCATGAAGAGGAAGCTTCAGGAGTTTCGAGTGACAGAGGATGCACTTCTTCCTGTTGGTACATCGCTTAATGTTCGCCATTTTGTTCCCGGACAGTATGTTGACATCACAGGAATCACTAAAGGAAAAGGTTTTCAGGTGTTTTTCTCTTTCCCTCATACTACATACTTCTCATTCCTTTTTTTCTTTATAAATAATAATCTCTCTCTTACATTGTTTATGTAGTATTTGTACATGTTTATGCTTGTATTGTTCCTGCTTGTGTCTATATAATACTTGAGTTGAGTTGAAATGCTTAGTTATTATTTTGTCAAAGAATTACATAATTGGGATTTTAAGCGTTTCGATCCACATCCAATTGGTCGGCAACAATAATAGGTTCACAAATGCATTATATGCCACTATTTATTTGTTCAATTGTTTTGTTTCCGATTTATTTACTTTTGGGCGATCATTCATGTTCAGTAGTTTCAACTGATCAGGTGATGTTGCACCGTTATTCTTTTATCTCCTCCCGTTTTATTTGACCCCTCCTGAAATTTGAACGACAAAATGTTGATTTTCATGCTTTCTAGGGGGTGTTTTTTTGTTTATTTTTTCTGTTTCTTATTTTATTTATAATCCATGTATGACTTCGGATACTTCTCTGTTTCATGCCGAAACCTTCCATCGAGTAAATCGGGCAATTACATGATACTACCATTTTTACAGATCTATGTCCTTTCAGTTACATGACTGTTAATTCAAATTATCTTCTGTTTACAGGGTGTAATGGACCGGTATGATTTCAAGGGAGGTTCAGCATCCCATGGGAATTCGAAAGCACATAGAACCATGGGTTCTTCAGGAATGCTTGGTCCTTGTAAGGTTGGATGGAGTCCTTTAGATTAGTATTTTTTCTTAATTAATACATCTTTCTGTATTTGTATATATTGAAAATATGTTTGATTGACTGTCTGTAAGTTTATTTCTTTACATTTATGTTTCTCACCTATAGTTGTGATAATATATTTTTCCTCACTGTTCAGGTTTTTAAAGGTAAAAAAATGCCGGGGAGAATGGGTGGGAAGCAAAGAACAGTAAAAAATGTATGGGTCTACAAAATTGATCCAGCAAGAAATTTGATGTGGGTGAAAGGCCAGGTAATTTTATATACGATTTATAAAATTGCAGCTATAGGACAGGAACTATTTTGTATCCCCTCGGGGAAAAAAAAAAACTGATTCAACTCGAGTGGTTAAAGAGCTTCAGTTAAAGATGAATCGATCTGAAGTATCCGAGTTTAAAACATGGCTCAAAGCCTGAAACAATCCTCATTCAAATTTTATTTACCTCTGGCTGAACTGTAGATTACTAGGGTTCCTTCCCTCACGGAACCGGAGGTTTAAGACCAAAAAATATCAACTAAGATAGTTGGCCATAATGATTTTAATTTTTTATTAAATTTGAATCCCACTTAATTCACATTGCAGTACTGCTAGTATAGAAACTTGTATGTTTATATGAGCTAAGAAATTTTGTATGACGATATCATTAAGAAAACGATGGTGAATCAGTAACCATTTTGTATCTATTTTAATATGCCAATTATTTATTAATAACTGGCCAAAATATTTGGAATGTTGGCTTTATGCGGGCTGATTGCTTTTCTTTGTAATTGTATGATGTGCACTATGCAGGTCCCAGGAGCTACAGGGAACTTTGTTTTTATAAAAGATGCAGTTTATGAAAAACCTGAGACATCTATACTTCCTTTCCCAACTTACTTTGTGCCAGAAGATGAAGATACAGATGATATGAAACCTCTGGTTGCTGATCTTGGGGATGTGGATCCATTTATGGTTACTGATTAA
Protein sequence

>MS.gene043435.t1

MLAFSRGVVSRLRQFPVVPLPRFLSSEVETIRRIEAKPSVMTPNSQRTGVIAVKCGMTALWDKWGARVPISILWVDDNIVSQVKTPEKEGYWSLQIGCGQKKEKHSTKPEVGHFRAQGVPMKRKLQEFRVTEDALLPVGTSLNVRHFVPGQYVDITGITKGKGFQGVMDRYDFKGGSASHGNSKAHRTMGSSGMLGPCKVFKGKKMPGRMGGKQRTVKNVWVYKIDPARNLMWVKGQVPGATGNFVFIKDAVYEKPETSILPFPTYFVPEDEDTDDMKPLVADLGDVDPFMVTD