AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001687

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001687.t1 MTR_1g093060 98.299 294 5 0 1 294 54 347 0.0 597
MS.gene001687.t1 MTR_6g006970 92.857 294 21 0 1 294 1 294 0.0 572
MS.gene001687.t1 MTR_2g090370 93.269 104 7 0 189 292 1 104 2.40e-68 213
MS.gene001687.t1 MTR_3g118210 41.063 207 116 6 49 251 58 262 6.36e-43 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001687.t1 AT3G17465 66.975 324 77 4 1 294 1 324 1.77e-147 417
MS.gene001687.t1 AT2G43030 38.835 206 122 4 49 251 57 261 4.59e-39 138

Find 83 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGGATGTGGATCCATTTA+TGG 0.158517 1.3:-64719422 MS.gene001687:CDS
ACACCAGTTCTTTGTGAATT+TGG 0.165812 1.3:+64722151 None:intergenic
TGACTGCTCATTGGGATAAA+TGG 0.166624 1.3:-64722108 MS.gene001687:CDS
CTCACTGTTCAGGTCTTTAA+AGG 0.228704 1.3:-64720221 MS.gene001687:intron
CTTTAATCAGTAACCATAAA+TGG 0.233502 1.3:+64719409 None:intergenic
GAATTCCAATTTCTATCCTT+TGG 0.239774 1.3:-64722078 MS.gene001687:CDS
AAACCTTTGGTTGCTGATCT+TGG 0.318983 1.3:-64719442 MS.gene001687:CDS
TCTACCCAAAGGATAGAAAT+TGG 0.334271 1.3:+64722073 None:intergenic
AACCTTTGGTTGCTGATCTT+GGG 0.341776 1.3:-64719441 MS.gene001687:CDS
ACCCACATCAAATTTCTTGC+TGG 0.343726 1.3:+64720128 None:intergenic
GTTCAGATGTGGAGACTATA+AGG 0.352256 1.3:-64722204 MS.gene001687:CDS
CATACTGCCCGGGAACAAAA+TGG 0.352881 1.3:+64721269 None:intergenic
CAGGGTGTAATGAAGAAGTA+TGG 0.352968 1.3:-64720449 MS.gene001687:CDS
GCCCTAAAATGACCCACTTC+AGG 0.379527 1.3:+64721379 None:intergenic
TCATCTGAATCTTCATCTTC+CGG 0.389387 1.3:+64719469 None:intergenic
TTCAGATGATATGAAACCTT+TGG 0.395479 1.3:-64719455 MS.gene001687:CDS
AATTCCAATTTCTATCCTTT+GGG 0.400773 1.3:-64722077 MS.gene001687:CDS
ATGAAGAAGTATGGATTTCA+TGG 0.403214 1.3:-64720440 MS.gene001687:CDS
GACTGCTCATTGGGATAAAT+GGG 0.404613 1.3:-64722107 MS.gene001687:CDS
GGTTGCTGATCTTGGGGATG+TGG 0.407346 1.3:-64719434 MS.gene001687:CDS
GAACAATAGCCTTCCTTCTC+AGG 0.410835 1.3:+64722022 None:intergenic
TAGAAAGATGCCAGGGAGAA+TGG 0.416986 1.3:-64720198 MS.gene001687:CDS
TGCTTCCCACCCATTCTCCC+TGG 0.421120 1.3:+64720188 None:intergenic
ACAAAGTTCCCTGTAGCTCC+TGG 0.431609 1.3:+64719559 None:intergenic
AGGAATACTTGGTCCTTGTA+AGG 0.431878 1.3:-64720366 MS.gene001687:intron
GATTGGTTGTGGACAGAAGA+AGG 0.441589 1.3:-64721419 MS.gene001687:CDS
ACACAGGTCCCAGGAGCTAC+AGG 0.448282 1.3:-64719568 MS.gene001687:intron
ACCTTTGGTTGCTGATCTTG+GGG 0.448994 1.3:-64719440 MS.gene001687:CDS
ATGTGGAATGACTGCTCATT+GGG 0.453159 1.3:-64722116 MS.gene001687:CDS
TCTTCCGGCACAAAGTAAGT+TGG 0.469195 1.3:+64719484 None:intergenic
AGATGACAACATTGTCTCTC+AGG 0.475183 1.3:-64722053 MS.gene001687:CDS
ACACCAAATTCACAAAGAAC+TGG 0.478282 1.3:-64722154 MS.gene001687:CDS
CTTCCGGCACAAAGTAAGTT+GGG 0.483444 1.3:+64719485 None:intergenic
AGAAAGATGCCAGGGAGAAT+GGG 0.487342 1.3:-64720197 MS.gene001687:CDS
AGAAATTTGATGTGGGTGAA+AGG 0.489286 1.3:-64720122 MS.gene001687:CDS
GGACTCCATCCAACCTTACA+AGG 0.491579 1.3:+64720353 None:intergenic
CATAGAACCATGGGATCTAC+AGG 0.492630 1.3:-64720386 MS.gene001687:CDS
AATCGCGGCAGTGGTGCCAC+GGG 0.495730 1.3:+64722232 None:intergenic
ACAGGAATCACCAAAGGAAA+AGG 0.499760 1.3:-64721238 MS.gene001687:CDS
AATGTGGAATGACTGCTCAT+TGG 0.503569 1.3:-64722117 MS.gene001687:CDS
ACATTAAGTGATGTACCAAC+AGG 0.507292 1.3:+64721295 None:intergenic
TCTTGAAGCTTCCTCTTCAT+CGG 0.518555 1.3:+64721346 None:intergenic
GAATTTGGTGTCATCACACT+AGG 0.526237 1.3:+64722166 None:intergenic
CTCAAGGAGTTCCGATGAAG+AGG 0.527266 1.3:-64721357 MS.gene001687:CDS
TTTGATGTGGGTGAAAGGCC+AGG 0.528326 1.3:-64720117 MS.gene001687:intron
AAAGGAAGTATAGATGTGTC+AGG 0.528945 1.3:+64719508 None:intergenic
GGAAGGCTATTGTTCTCTCC+AGG 0.530451 1.3:-64722014 MS.gene001687:intron
ATGGGATCTACAGGAATACT+TGG 0.533632 1.3:-64720377 MS.gene001687:CDS
TGTGATGTCAACATACTGCC+CGG 0.537181 1.3:+64721258 None:intergenic
AAATCGCGGCAGTGGTGCCA+CGG 0.542479 1.3:+64722231 None:intergenic
GAGGATGCACTTCTTCCTGT+TGG 0.552055 1.3:-64721310 MS.gene001687:CDS
TTTCCCAACTTACTTTGTGC+CGG 0.552953 1.3:-64719488 MS.gene001687:CDS
CAAAGTTCCCTGTAGCTCCT+GGG 0.553971 1.3:+64719560 None:intergenic
CATGGAGGTTCAGCATCCCA+TGG 0.557351 1.3:-64720422 MS.gene001687:CDS
ACACTTGACAAAGCCTGAAG+TGG 0.560843 1.3:-64721392 MS.gene001687:CDS
TTTAAAGGTAGAAAGATGCC+AGG 0.564006 1.3:-64720206 MS.gene001687:CDS
TCAAAAGCACATAGAACCAT+GGG 0.566671 1.3:-64720395 MS.gene001687:CDS
TCAGGTCAAAACTCCTGAGA+AGG 0.567631 1.3:-64722035 MS.gene001687:CDS
AAGTATTCCTGTAGATCCCA+TGG 0.577273 1.3:+64720379 None:intergenic
TCAAGAGTTTCGAGTGACAG+AGG 0.581725 1.3:-64721329 MS.gene001687:CDS
GTGTGCACAACACAGGTCCC+AGG 0.582644 1.3:-64719577 MS.gene001687:intron
TTCAAAAGCACATAGAACCA+TGG 0.583765 1.3:-64720396 MS.gene001687:CDS
ATGGAGGTTCAGCATCCCAT+GGG 0.591854 1.3:-64720421 MS.gene001687:CDS
GGGCAGTATGTTGACATCAC+AGG 0.594209 1.3:-64721256 MS.gene001687:CDS
AAGATGCCAGGGAGAATGGG+TGG 0.599236 1.3:-64720194 MS.gene001687:CDS
GGCACAAAGTAAGTTGGGAA+AGG 0.607369 1.3:+64719490 None:intergenic
ACCTCTTGAAAACGCCAGCA+TGG 0.609546 1.3:+64722279 None:intergenic
CACTTGACAAAGCCTGAAGT+GGG 0.609676 1.3:-64721391 MS.gene001687:CDS
GTCAAAACTCCTGAGAAGGA+AGG 0.621009 1.3:-64722031 MS.gene001687:CDS
ATCCAGCAAGAAATTTGATG+TGG 0.622717 1.3:-64720130 MS.gene001687:CDS
CACAGGTCCCAGGAGCTACA+GGG 0.625211 1.3:-64719567 MS.gene001687:intron
GTGATGTCAACATACTGCCC+GGG 0.631463 1.3:+64721259 None:intergenic
AGATGCCAGGGAGAATGGGT+GGG 0.644345 1.3:-64720193 MS.gene001687:CDS
GGTGTTATAGCTGTAAAATG+TGG 0.647527 1.3:-64722133 MS.gene001687:CDS
TCCCCAAGATCAGCAACCAA+AGG 0.647605 1.3:+64719439 None:intergenic
GACATCACAGGAATCACCAA+AGG 0.651363 1.3:-64721244 MS.gene001687:CDS
CAATGTTGTCATCTACCCAA+AGG 0.657641 1.3:+64722062 None:intergenic
TTAAAGGTAGAAAGATGCCA+GGG 0.664670 1.3:-64720205 MS.gene001687:CDS
TCCAGCAAGAAATTTGATGT+GGG 0.677547 1.3:-64720129 MS.gene001687:CDS
AAGAAGTATGGATTTCATGG+AGG 0.684184 1.3:-64720437 MS.gene001687:CDS
ATCGCGGCAGTGGTGCCACG+GGG 0.726950 1.3:+64722233 None:intergenic
TCGTCTTCGACAATTCCCCG+TGG 0.741512 1.3:-64722248 MS.gene001687:CDS
ACTGCTCATTGGGATAAATG+GGG 0.750912 1.3:-64722106 MS.gene001687:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTAAATTTGTATTTGT+AGG - chr1.3:64720685-64720704 MS.gene001687:intron 10.0%
!! AATGTTTATAATGAATAATG+CGG + chr1.3:64719826-64719845 None:intergenic 15.0%
!! CTACTAAATTAAATACAAAA+TGG + chr1.3:64722017-64722036 None:intergenic 15.0%
!! GACAGAAATTATTATTTATA+TGG + chr1.3:64720527-64720546 None:intergenic 15.0%
!! GTTTATAATGAGTTATTAAA+TGG - chr1.3:64720133-64720152 MS.gene001687:CDS 15.0%
!! TTGAACAAAACAATAAATAA+TGG + chr1.3:64720875-64720894 None:intergenic 15.0%
!! TTGATTACATTAGTTATATT+AGG - chr1.3:64720754-64720773 MS.gene001687:intron 15.0%
!! AAAGAACAGTAAAAAATGTA+TGG - chr1.3:64721522-64721541 MS.gene001687:intron 20.0%
!! AAGAACAGTAAAAAATGTAT+GGG - chr1.3:64721523-64721542 MS.gene001687:intron 20.0%
!! ACAAAAAAACAATCATCTTA+GGG + chr1.3:64720725-64720744 None:intergenic 20.0%
!! CAAATTCAATCATGTATTAT+AGG - chr1.3:64720849-64720868 MS.gene001687:intron 20.0%
!! GCAGAAAAATAAACAATAAA+TGG + chr1.3:64721089-64721108 None:intergenic 20.0%
!! TACAAAAAAACAATCATCTT+AGG + chr1.3:64720726-64720745 None:intergenic 20.0%
!! TATATATAGAAGACATATCT+AGG + chr1.3:64720190-64720209 None:intergenic 20.0%
!! TGTCAACTTATTATATCATT+AGG + chr1.3:64720831-64720850 None:intergenic 20.0%
!! TTATTATATCATTAGGATTC+AGG + chr1.3:64720824-64720843 None:intergenic 20.0%
!!! AAATTATATTTTCTGTTTCC+AGG - chr1.3:64721220-64721239 MS.gene001687:intron 20.0%
!!! AAGATGATTGTTTTTTTGTA+AGG - chr1.3:64720726-64720745 MS.gene001687:intron 20.0%
!!! AATTATATTTTCTGTTTCCA+GGG - chr1.3:64721221-64721240 MS.gene001687:intron 20.0%
!!! AGATGATTGTTTTTTTGTAA+GGG - chr1.3:64720727-64720746 MS.gene001687:intron 20.0%
!!! TTTTGATGTACAAAATTTCT+TGG + chr1.3:64721952-64721971 None:intergenic 20.0%
! AACATGTAAAAACAATTACC+TGG + chr1.3:64721592-64721611 None:intergenic 25.0%
! AACTAAGAATACTAATCTGA+AGG + chr1.3:64721359-64721378 None:intergenic 25.0%
! AATTCCAATTTCTATCCTTT+GGG - chr1.3:64719611-64719630 MS.gene001687:intron 25.0%
! ACAGTAAAAAAAGCAGAATA+TGG - chr1.3:64720077-64720096 MS.gene001687:intron 25.0%
! ATTTACTTCTAGACAATCAT+CGG - chr1.3:64720916-64720935 MS.gene001687:intron 25.0%
! CACACATTAATTTATGAACT+TGG - chr1.3:64721615-64721634 MS.gene001687:intron 25.0%
! TAGCTCAATTATTCATTAAC+TGG - chr1.3:64722034-64722053 MS.gene001687:CDS 25.0%
! TTGAATTTCACCAATCTAAT+CGG + chr1.3:64720112-64720131 None:intergenic 25.0%
!! ATATGAAAGTATGTAATACG+AGG + chr1.3:64720498-64720517 None:intergenic 25.0%
!! GGCAAAAATATTTGAAATCT+CGG - chr1.3:64722055-64722074 MS.gene001687:CDS 25.0%
!! TATGAAAGTATGTAATACGA+GGG + chr1.3:64720497-64720516 None:intergenic 25.0%
!!! ATGCTTTGTTTTAGTTAGAT+TGG - chr1.3:64720252-64720271 MS.gene001687:intron 25.0%
!!! GTTACACGTTTTTTTTTGAA+TGG - chr1.3:64721176-64721195 MS.gene001687:intron 25.0%
AAAAAAACGTGTAACTGACA+AGG + chr1.3:64721171-64721190 None:intergenic 30.0%
AATCCACATGAAACTCATTA+GGG + chr1.3:64719736-64719755 None:intergenic 30.0%
AATGCTTATTCCTCTTTGTA+TGG - chr1.3:64722089-64722108 MS.gene001687:CDS 30.0%
ACATCTGAACTAAAAAATCG+CGG + chr1.3:64719474-64719493 None:intergenic 30.0%
ACTTGATCCTTAACATAGTA+TGG - chr1.3:64720023-64720042 MS.gene001687:intron 30.0%
ATTATCGCCATACTATGTTA+AGG + chr1.3:64720033-64720052 None:intergenic 30.0%
ATTTATGTTTCTCACTGTTC+AGG - chr1.3:64721457-64721476 MS.gene001687:intron 30.0%
CGAAAATTTGTGTAGTTGAT+TGG + chr1.3:64719862-64719881 None:intergenic 30.0%
CGTTTGTTTATTGACATGAT+TGG - chr1.3:64720643-64720662 MS.gene001687:intron 30.0%
GAATTCCAATTTCTATCCTT+TGG - chr1.3:64719610-64719629 MS.gene001687:intron 30.0%
GATTGCATAGATACAAATTC+TGG - chr1.3:64719980-64719999 MS.gene001687:intron 30.0%
GGATGAATCAATCATATTCA+TGG - chr1.3:64721721-64721740 MS.gene001687:intron 30.0%
TAATATCATCAAGAGAACGA+CGG - chr1.3:64721981-64722000 MS.gene001687:intron 30.0%
TAATCCACATGAAACTCATT+AGG + chr1.3:64719737-64719756 None:intergenic 30.0%
TACATGTTTATGCTTGTATG+TGG - chr1.3:64720570-64720589 MS.gene001687:intron 30.0%
TTCAGATGATATGAAACCTT+TGG - chr1.3:64722233-64722252 MS.gene001687:CDS 30.0%
TTGAAATCTCGGATTTATCA+CGG - chr1.3:64722066-64722085 MS.gene001687:CDS 30.0%
TTGTCATTCATGTATGATTG+CGG - chr1.3:64721121-64721140 MS.gene001687:intron 30.0%
TTGTGTAGTTGATTGGATTT+CGG + chr1.3:64719855-64719874 None:intergenic 30.0%
! ATGAAGAAGTATGGATTTCA+TGG - chr1.3:64721248-64721267 MS.gene001687:CDS 30.0%
!! AATCACTTGATTTGTGTATG+AGG + chr1.3:64721670-64721689 None:intergenic 30.0%
!! GGGTAAGTAAAGTTTTAACA+TGG + chr1.3:64721757-64721776 None:intergenic 30.0%
!!! TGTTTTAGTTAGATTGGTTG+TGG - chr1.3:64720258-64720277 MS.gene001687:intron 30.0%
ACACCAAATTCACAAAGAAC+TGG - chr1.3:64719534-64719553 MS.gene001687:CDS 35.0%
ACACCAGTTCTTTGTGAATT+TGG + chr1.3:64719540-64719559 None:intergenic 35.0%
ACATTAAGTGATGTACCAAC+AGG + chr1.3:64720396-64720415 None:intergenic 35.0%
AGAAATTTGATGTGGGTGAA+AGG - chr1.3:64721566-64721585 MS.gene001687:intron 35.0%
ATCCAGCAAGAAATTTGATG+TGG - chr1.3:64721558-64721577 MS.gene001687:intron 35.0%
ATTTATGAACTTGGAGCTCT+AGG - chr1.3:64721624-64721643 MS.gene001687:intron 35.0%
CATAGTATGGCGATAATATC+TGG - chr1.3:64720036-64720055 MS.gene001687:intron 35.0%
GAGTGGCAACTTATTGTTAT+TGG + chr1.3:64720793-64720812 None:intergenic 35.0%
GCGATAATATCTGGTTAGAT+TGG - chr1.3:64720045-64720064 MS.gene001687:intron 35.0%
TAATCCGGAGTTCAATCAAA+GGG + chr1.3:64721777-64721796 None:intergenic 35.0%
TATCCCTAATGAGTTTCATG+TGG - chr1.3:64719730-64719749 MS.gene001687:intron 35.0%
TCATCTGAATCTTCATCTTC+CGG + chr1.3:64722222-64722241 None:intergenic 35.0%
TCATTAATGACCAAGTGTCT+TGG + chr1.3:64721847-64721866 None:intergenic 35.0%
TCCAGCAAGAAATTTGATGT+GGG - chr1.3:64721559-64721578 MS.gene001687:intron 35.0%
TCTACCCAAAGGATAGAAAT+TGG + chr1.3:64719618-64719637 None:intergenic 35.0%
TGCGGAGTAACTAAACTAAA+AGG + chr1.3:64719808-64719827 None:intergenic 35.0%
TGCTATTATATAGACACGTG+CGG + chr1.3:64720598-64720617 None:intergenic 35.0%
TTAAAGGTAGAAAGATGCCA+GGG - chr1.3:64721483-64721502 MS.gene001687:intron 35.0%
TTTAAAGGTAGAAAGATGCC+AGG - chr1.3:64721482-64721501 MS.gene001687:intron 35.0%
TTTATGAACTTGGAGCTCTA+GGG - chr1.3:64721625-64721644 MS.gene001687:intron 35.0%
! AAAGGAAGTATAGATGTGTC+AGG + chr1.3:64722183-64722202 None:intergenic 35.0%
! AAGAAGTATGGATTTCATGG+AGG - chr1.3:64721251-64721270 MS.gene001687:CDS 35.0%
! ATGTGCTTTTGAATTCCCAT+GGG + chr1.3:64721285-64721304 None:intergenic 35.0%
! GGTGTTATAGCTGTAAAATG+TGG - chr1.3:64719555-64719574 MS.gene001687:CDS 35.0%
! TATGTGCTTTTGAATTCCCA+TGG + chr1.3:64721286-64721305 None:intergenic 35.0%
! TCAAAAGCACATAGAACCAT+GGG - chr1.3:64721293-64721312 MS.gene001687:CDS 35.0%
! TTCAAAAGCACATAGAACCA+TGG - chr1.3:64721292-64721311 MS.gene001687:CDS 35.0%
! TTTTTGAATGGCAGTTACAC+AGG - chr1.3:64721188-64721207 MS.gene001687:intron 35.0%
!! GGTTGATGAGTTTGAGTTAA+AGG - chr1.3:64721700-64721719 MS.gene001687:intron 35.0%
!!! GCGATTTTTTAGTTCAGATG+TGG - chr1.3:64719473-64719492 MS.gene001687:CDS 35.0%
AAACCTTTGGTTGCTGATCT+TGG - chr1.3:64722246-64722265 MS.gene001687:CDS 40.0%
AACCTTTGGTTGCTGATCTT+GGG - chr1.3:64722247-64722266 MS.gene001687:CDS 40.0%
AACTGCAAATGTGCAAATGG+TGG + chr1.3:64721896-64721915 None:intergenic 40.0%
AAGACATATCTAGGTACTCC+AGG + chr1.3:64720181-64720200 None:intergenic 40.0%
AAGTATTCCTGTAGATCCCA+TGG + chr1.3:64721312-64721331 None:intergenic 40.0%
AATCTTGCACGTACAAGTTC+AGG - chr1.3:64720966-64720985 MS.gene001687:intron 40.0%
AATGTGGAATGACTGCTCAT+TGG - chr1.3:64719571-64719590 MS.gene001687:intron 40.0%
ACAGGAATCACCAAAGGAAA+AGG - chr1.3:64720450-64720469 MS.gene001687:intron 40.0%
ACCCACATCAAATTTCTTGC+TGG + chr1.3:64721563-64721582 None:intergenic 40.0%
ACTGCAAATGTGCAAATGGT+GGG + chr1.3:64721895-64721914 None:intergenic 40.0%
ACTGCTCATTGGGATAAATG+GGG - chr1.3:64719582-64719601 MS.gene001687:intron 40.0%
AGATGACAACATTGTCTCTC+AGG - chr1.3:64719635-64719654 MS.gene001687:intron 40.0%
AGGAATACTTGGTCCTTGTA+AGG - chr1.3:64721322-64721341 MS.gene001687:CDS 40.0%
ATACTTGGTCCTTGTAAGGT+TGG - chr1.3:64721326-64721345 MS.gene001687:CDS 40.0%
ATGGGATCTACAGGAATACT+TGG - chr1.3:64721311-64721330 MS.gene001687:CDS 40.0%
ATGTGGAATGACTGCTCATT+GGG - chr1.3:64719572-64719591 MS.gene001687:intron 40.0%
ATTCAGCTTAGAGTTACTCC+TGG - chr1.3:64720160-64720179 MS.gene001687:CDS 40.0%
CAATGTTGTCATCTACCCAA+AGG + chr1.3:64719629-64719648 None:intergenic 40.0%
CAGGGTGTAATGAAGAAGTA+TGG - chr1.3:64721239-64721258 MS.gene001687:CDS 40.0%
CATACTTCTTCATTACACCC+TGG + chr1.3:64721241-64721260 None:intergenic 40.0%
CCAATATCAACCAAGACACT+TGG - chr1.3:64721834-64721853 MS.gene001687:intron 40.0%
CCATTTGCACATTTGCAGTT+CGG - chr1.3:64721896-64721915 MS.gene001687:intron 40.0%
CTCACTGTTCAGGTCTTTAA+AGG - chr1.3:64721467-64721486 MS.gene001687:intron 40.0%
CTTACCCTTTGATTGAACTC+CGG - chr1.3:64721770-64721789 MS.gene001687:intron 40.0%
GACTGCTCATTGGGATAAAT+GGG - chr1.3:64719581-64719600 MS.gene001687:intron 40.0%
GATTGAACTCCGGATTACTA+GGG - chr1.3:64721780-64721799 MS.gene001687:intron 40.0%
GCTATTATATAGACACGTGC+GGG + chr1.3:64720597-64720616 None:intergenic 40.0%
GTAATCCGGAGTTCAATCAA+AGG + chr1.3:64721778-64721797 None:intergenic 40.0%
GTGACAGTATCCGATTAGAT+TGG - chr1.3:64720099-64720118 MS.gene001687:intron 40.0%
GTTCAGATGTGGAGACTATA+AGG - chr1.3:64719484-64719503 MS.gene001687:CDS 40.0%
TCTTGAAGCTTCCTCTTCAT+CGG + chr1.3:64720345-64720364 None:intergenic 40.0%
TGACTGCTCATTGGGATAAA+TGG - chr1.3:64719580-64719599 MS.gene001687:intron 40.0%
TGATTGAACTCCGGATTACT+AGG - chr1.3:64721779-64721798 MS.gene001687:intron 40.0%
TGCGTTTCAAATTTCCAGAG+AGG + chr1.3:64721036-64721055 None:intergenic 40.0%
TTCAAATTTCCAGAGAGGTC+AGG + chr1.3:64721031-64721050 None:intergenic 40.0%
TTTCCCAACTTACTTTGTGC+CGG - chr1.3:64722200-64722219 MS.gene001687:CDS 40.0%
! AACGCAAAATGTTGAGTTGC+AGG - chr1.3:64721050-64721069 MS.gene001687:intron 40.0%
! CACCAAAGGAAAAGGTTTTC+AGG - chr1.3:64720458-64720477 MS.gene001687:intron 40.0%
! CACCTGAAAACCTTTTCCTT+TGG + chr1.3:64720463-64720482 None:intergenic 40.0%
! CCAAGTGTCTTGGTTGATAT+TGG + chr1.3:64721837-64721856 None:intergenic 40.0%
! TTATGAACTTGGAGCTCTAG+GGG - chr1.3:64721626-64721645 MS.gene001687:intron 40.0%
!! AATGTTCGCCATTTTGTTCC+CGG - chr1.3:64720411-64720430 MS.gene001687:CDS 40.0%
!! GAATTTGGTGTCATCACACT+AGG + chr1.3:64719525-64719544 None:intergenic 40.0%
!! TAGCAGTTGAGTTGAATGCA+TGG - chr1.3:64720613-64720632 MS.gene001687:intron 40.0%
ACACTTGACAAAGCCTGAAG+TGG - chr1.3:64720296-64720315 MS.gene001687:intron 45.0%
ACCTTTGGTTGCTGATCTTG+GGG - chr1.3:64722248-64722267 MS.gene001687:CDS 45.0%
AGAAAGATGCCAGGGAGAAT+GGG - chr1.3:64721491-64721510 MS.gene001687:intron 45.0%
AGAATGAAGCAGAACACACC+TGG + chr1.3:64719695-64719714 None:intergenic 45.0%
ATCAAGTGATTGAGCTCGAG+CGG - chr1.3:64721679-64721698 MS.gene001687:intron 45.0%
CACTTGACAAAGCCTGAAGT+GGG - chr1.3:64720297-64720316 MS.gene001687:intron 45.0%
CATAGAACCATGGGATCTAC+AGG - chr1.3:64721302-64721321 MS.gene001687:CDS 45.0%
CCAGAGAGGTCAGGTAAAAT+GGG + chr1.3:64721022-64721041 None:intergenic 45.0%
CCGAACTGCAAATGTGCAAA+TGG + chr1.3:64721899-64721918 None:intergenic 45.0%
CTTCCGGCACAAAGTAAGTT+GGG + chr1.3:64722206-64722225 None:intergenic 45.0%
GAACAATAGCCTTCCTTCTC+AGG + chr1.3:64719669-64719688 None:intergenic 45.0%
GAACTAAAAAATCGCGGCAG+TGG + chr1.3:64719468-64719487 None:intergenic 45.0%
GACATCACAGGAATCACCAA+AGG - chr1.3:64720444-64720463 MS.gene001687:CDS 45.0%
GCACGTACAAGTTCAGGTAA+TGG - chr1.3:64720972-64720991 MS.gene001687:intron 45.0%
GTCAAAACTCCTGAGAAGGA+AGG - chr1.3:64719657-64719676 MS.gene001687:intron 45.0%
GTGTGTTCTGCTTCATTCTC+AGG - chr1.3:64719696-64719715 MS.gene001687:intron 45.0%
GTTGTGCACACCATACAAAG+AGG + chr1.3:64722102-64722121 None:intergenic 45.0%
TAGAAAGATGCCAGGGAGAA+TGG - chr1.3:64721490-64721509 MS.gene001687:intron 45.0%
TATCATCAAGAGAACGACGG+TGG - chr1.3:64721984-64722003 MS.gene001687:intron 45.0%
TCAGGTCAAAACTCCTGAGA+AGG - chr1.3:64719653-64719672 MS.gene001687:intron 45.0%
TCCAGAGAGGTCAGGTAAAA+TGG + chr1.3:64721023-64721042 None:intergenic 45.0%
TCTTCCGGCACAAAGTAAGT+TGG + chr1.3:64722207-64722226 None:intergenic 45.0%
TGGGGATGTGGATCCATTTA+TGG - chr1.3:64722266-64722285 MS.gene001687:CDS 45.0%
TGTGATGTCAACATACTGCC+CGG + chr1.3:64720433-64720452 None:intergenic 45.0%
TTGGTCCTTGTAAGGTTGGA+TGG - chr1.3:64721330-64721349 MS.gene001687:CDS 45.0%
TTGTATGGTGTGCACAACAC+AGG - chr1.3:64722104-64722123 MS.gene001687:CDS 45.0%
! GATTGGTTGTGGACAGAAGA+AGG - chr1.3:64720269-64720288 MS.gene001687:intron 45.0%
! GGCACAAAGTAAGTTGGGAA+AGG + chr1.3:64722201-64722220 None:intergenic 45.0%
! GTTGAGTTGCAGGCTTTCTA+GGG - chr1.3:64721060-64721079 MS.gene001687:intron 45.0%
! TACTAGGGCTCCTTCCTTTT+GGG - chr1.3:64721795-64721814 MS.gene001687:intron 45.0%
! TCAAGAGTTTCGAGTGACAG+AGG - chr1.3:64720359-64720378 MS.gene001687:intron 45.0%
! TGTTGAGTTGCAGGCTTTCT+AGG - chr1.3:64721059-64721078 MS.gene001687:intron 45.0%
! TTACTAGGGCTCCTTCCTTT+TGG - chr1.3:64721794-64721813 MS.gene001687:intron 45.0%
! TTGAGTTGCAGGCTTTCTAG+GGG - chr1.3:64721061-64721080 MS.gene001687:intron 45.0%
!! AGCCTGAAGTGGGTCATTTT+AGG - chr1.3:64720307-64720326 MS.gene001687:intron 45.0%
!! ATGTTCGCCATTTTGTTCCC+GGG - chr1.3:64720412-64720431 MS.gene001687:CDS 45.0%
!! GCCTGAAGTGGGTCATTTTA+GGG - chr1.3:64720308-64720327 MS.gene001687:intron 45.0%
!! TGATATTGGCCTTGACTCTC+CGG + chr1.3:64721823-64721842 None:intergenic 45.0%
ACAAAGTTCCCTGTAGCTCC+TGG + chr1.3:64722132-64722151 None:intergenic 50.0%
AGGAAGGAGCCCTAGTAATC+CGG + chr1.3:64721792-64721811 None:intergenic 50.0%
ATGGAGGTTCAGCATCCCAT+GGG - chr1.3:64721267-64721286 MS.gene001687:CDS 50.0%
CAAAGTTCCCTGTAGCTCCT+GGG + chr1.3:64722131-64722150 None:intergenic 50.0%
CATACTGCCCGGGAACAAAA+TGG + chr1.3:64720422-64720441 None:intergenic 50.0%
CTCAAGGAGTTCCGATGAAG+AGG - chr1.3:64720331-64720350 MS.gene001687:intron 50.0%
GAGGATGCACTTCTTCCTGT+TGG - chr1.3:64720378-64720397 MS.gene001687:CDS 50.0%
GCCCTAAAATGACCCACTTC+AGG + chr1.3:64720312-64720331 None:intergenic 50.0%
GGAAGGCTATTGTTCTCTCC+AGG - chr1.3:64719674-64719693 MS.gene001687:intron 50.0%
GGACTCCATCCAACCTTACA+AGG + chr1.3:64721338-64721357 None:intergenic 50.0%
GGGCAGTATGTTGACATCAC+AGG - chr1.3:64720432-64720451 MS.gene001687:CDS 50.0%
GTGATGTCAACATACTGCCC+GGG + chr1.3:64720432-64720451 None:intergenic 50.0%
TCCCCAAGATCAGCAACCAA+AGG + chr1.3:64722252-64722271 None:intergenic 50.0%
TGAGTTGCAGGCTTTCTAGG+GGG - chr1.3:64721062-64721081 MS.gene001687:intron 50.0%
TTGACTCTCCGGTTCCCAAA+AGG + chr1.3:64721812-64721831 None:intergenic 50.0%
TTTGATGTGGGTGAAAGGCC+AGG - chr1.3:64721571-64721590 MS.gene001687:intron 50.0%
! CCCATTTTACCTGACCTCTC+TGG - chr1.3:64721019-64721038 MS.gene001687:intron 50.0%
! GGATTCAGGACTCTGAAGAG+TGG + chr1.3:64720810-64720829 None:intergenic 50.0%
!! TTTTGGGAACCGGAGAGTCA+AGG - chr1.3:64721811-64721830 MS.gene001687:intron 50.0%
!!! GTGGGTCATTTTAGGGCTCA+AGG - chr1.3:64720315-64720334 MS.gene001687:intron 50.0%
AAGATGCCAGGGAGAATGGG+TGG - chr1.3:64721494-64721513 MS.gene001687:intron 55.0%
AGATGCCAGGGAGAATGGGT+GGG - chr1.3:64721495-64721514 MS.gene001687:intron 55.0%
CATGGAGGTTCAGCATCCCA+TGG - chr1.3:64721266-64721285 MS.gene001687:CDS 55.0%
CTCTCCGGTTCCCAAAAGGA+AGG + chr1.3:64721808-64721827 None:intergenic 55.0%
GGTTGCTGATCTTGGGGATG+TGG - chr1.3:64722254-64722273 MS.gene001687:CDS 55.0%
TCGTCTTCGACAATTCCCCG+TGG - chr1.3:64719440-64719459 MS.gene001687:CDS 55.0%
! GAGTTGCAGGCTTTCTAGGG+GGG - chr1.3:64721063-64721082 MS.gene001687:intron 55.0%
!! GCTGGCGTTTTCAAGAGGTG+TGG - chr1.3:64719413-64719432 MS.gene001687:CDS 55.0%
!! GGCTCCTTCCTTTTGGGAAC+CGG - chr1.3:64721801-64721820 MS.gene001687:intron 55.0%
ACACAGGTCCCAGGAGCTAC+AGG - chr1.3:64722120-64722139 MS.gene001687:CDS 60.0%
CACAGGTCCCAGGAGCTACA+GGG - chr1.3:64722121-64722140 MS.gene001687:CDS 60.0%
GTGTGCACAACACAGGTCCC+AGG - chr1.3:64722111-64722130 MS.gene001687:CDS 60.0%
TGCTTCCCACCCATTCTCCC+TGG + chr1.3:64721503-64721522 None:intergenic 60.0%
! AAATCGCGGCAGTGGTGCCA+CGG + chr1.3:64719460-64719479 None:intergenic 60.0%
!! AATCGCGGCAGTGGTGCCAC+GGG + chr1.3:64719459-64719478 None:intergenic 65.0%
!! ATCGCGGCAGTGGTGCCACG+GGG + chr1.3:64719458-64719477 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 64719411 64722299 64719411 ID=MS.gene001687
chr1.3 mRNA 64719411 64722299 64719411 ID=MS.gene001687.t1;Parent=MS.gene001687
chr1.3 exon 64722015 64722299 64722015 ID=MS.gene001687.t1.exon1;Parent=MS.gene001687.t1
chr1.3 CDS 64722015 64722299 64722015 ID=cds.MS.gene001687.t1;Parent=MS.gene001687.t1
chr1.3 exon 64721231 64721440 64721231 ID=MS.gene001687.t1.exon2;Parent=MS.gene001687.t1
chr1.3 CDS 64721231 64721440 64721231 ID=cds.MS.gene001687.t1;Parent=MS.gene001687.t1
chr1.3 exon 64720367 64720468 64720367 ID=MS.gene001687.t1.exon3;Parent=MS.gene001687.t1
chr1.3 CDS 64720367 64720468 64720367 ID=cds.MS.gene001687.t1;Parent=MS.gene001687.t1
chr1.3 exon 64720118 64720231 64720118 ID=MS.gene001687.t1.exon4;Parent=MS.gene001687.t1
chr1.3 CDS 64720118 64720231 64720118 ID=cds.MS.gene001687.t1;Parent=MS.gene001687.t1
chr1.3 exon 64719411 64719584 64719411 ID=MS.gene001687.t1.exon5;Parent=MS.gene001687.t1
chr1.3 CDS 64719411 64719584 64719411 ID=cds.MS.gene001687.t1;Parent=MS.gene001687.t1
Gene Sequence

>MS.gene001687

ATGCTGGCGTTTTCAAGAGGTGTGGTTTCTCGTCTTCGACAATTCCCCGTGGCACCACTGCCGCGATTTTTTAGTTCAGATGTGGAGACTATAAGGACGATTGAAGCAAAACCTAGTGTGATGACACCAAATTCACAAAGAACTGGTGTTATAGCTGTAAAATGTGGAATGACTGCTCATTGGGATAAATGGGGTGCTAGAATTCCAATTTCTATCCTTTGGGTAGATGACAACATTGTCTCTCAGGTCAAAACTCCTGAGAAGGAAGGCTATTGTTCTCTCCAGGTGTGTTCTGCTTCATTCTCAGGATCAAATTCAATATCCCTAATGAGTTTCATGTGGATTAACTCAGTTCAGAGTTACTTATTGAATTACTTAAGTTACTGCGACAATACCTTTTAGTTTAGTTACTCCGCATTATTCATTATAAACATTGAATTTCCGAAATCCAATCAACTACACAAATTTTCGAAAGAAATTAAAACTATTTGAAAGTTCATTAAAGAACTTCAAATGAACGTATAGTTATTTATTTTATTTTTTTAAATGCATGTAAACATCGTCTGCTAGATTGCATAGATACAAATTCTGGAATTATATGAAACTATGTGTACTTGATCCTTAACATAGTATGGCGATAATATCTGGTTAGATTGGTGAAATAATACAGTAAAAAAAGCAGAATATGGTGACAGTATCCGATTAGATTGGTGAAATTCAATGTTTATAATGAGTTATTAAATGGAATAATTCAGCTTAGAGTTACTCCTGGAGTACCTAGATATGTCTTCTATATATATGTATATAAAGTTAAATTTGTATCTAATTATAGACATGTTTGATGCTTTGTTTTAGTTAGATTGGTTGTGGACAGAAGAAGGAAAAACACTTGACAAAGCCTGAAGTGGGTCATTTTAGGGCTCAAGGAGTTCCGATGAAGAGGAAGCTTCAAGAGTTTCGAGTGACAGAGGATGCACTTCTTCCTGTTGGTACATCACTTAATGTTCGCCATTTTGTTCCCGGGCAGTATGTTGACATCACAGGAATCACCAAAGGAAAAGGTTTTCAGGTGTTTTTCTTTTTCCCTCGTATTACATACTTTCATATTGTTTTCCATATAAATAATAATTTCTGTCTTACATTGAGTATGTAGCATGTGTACATGTTTATGCTTGTATGTGGTCCCGCACGTGTCTATATAATAGCAGTTGAGTTGAATGCATGGTGTTCTTCGTTTGTTTATTGACATGATTGGTTATTATTTTGTCAAAGAATTTATTAAATTTGTATTTGTAGGATTAAAATGAATTACCCTAAGATGATTGTTTTTTTGTAAGGGCTAGTTGATTACATTAGTTATATTAGGATTTTAAGTGTTGCCAATAACAATAAGTTGCCACTCTTCAGAGTCCTGAATCCTAATGATATAATAAGTTGACAAATTCAATCATGTATTATAGGCCATTATTTATTGTTTTGTTCAATTGTTTTCTGTTTCACGATTTATTTACTTCTAGACAATCATCGGTGTTCATTAGTTTCAACTTTCAACTATAATCTTGCACGTACAAGTTCAGGTAATGGTGCAACTTTATTCTTTTCTCTCTTCCCATTTTACCTGACCTCTCTGGAAATTTGAAACGCAAAATGTTGAGTTGCAGGCTTTCTAGGGGGGCCATTTATTGTTTATTTTTCTGCTTCAAAATGTATTTGTCATTCATGTATGATTGCGGATACTTCTCTGTTTCATGTTGAAACCTTGTCAGTTACACGTTTTTTTTTGAATGGCAGTTACACAGGAGTTAATTTAAATTATATTTTCTGTTTCCAGGGTGTAATGAAGAAGTATGGATTTCATGGAGGTTCAGCATCCCATGGGAATTCAAAAGCACATAGAACCATGGGATCTACAGGAATACTTGGTCCTTGTAAGGTTGGATGGAGTCCTTCAGATTAGTATTCTTAGTTAATACAGCTTTCTCTATTTGTACGTATTGAAATCATGTTAGATTGACTGTCTGTATAAGTTTATTTTCTTTGCATTACATTTATGTTTCTCACTGTTCAGGTCTTTAAAGGTAGAAAGATGCCAGGGAGAATGGGTGGGAAGCAAAGAACAGTAAAAAATGTATGGGTCTACAAAATTGATCCAGCAAGAAATTTGATGTGGGTGAAAGGCCAGGTAATTGTTTTTACATGTTTATCACACATTAATTTATGAACTTGGAGCTCTAGGGGAGTAACTATCTTATATCACCTCATACACAAATCAAGTGATTGAGCTCGAGCGGTTGATGAGTTTGAGTTAAAGGATGAATCAATCATATTCATGGCTGAAACAATCCATGTTAAAACTTTACTTACCCTTTGATTGAACTCCGGATTACTAGGGCTCCTTCCTTTTGGGAACCGGAGAGTCAAGGCCAATATCAACCAAGACACTTGGTCATTAATGATTTTAAATTTTTATTAAATTTGAATCCCACCATTTGCACATTTGCAGTTCGGCTAGTACAGAGACTTGTATGTTTGTGTGAGCCAAGAAATTTTGTACATCAAAAAATTCTGACTAATATCATCAAGAGAACGACGGTGGATTGAAACCATTTTGTATTTAATTTAGTAGCTCAATTATTCATTAACTGGCAAAAATATTTGAAATCTCGGATTTATCACGGAATGCTTATTCCTCTTTGTATGGTGTGCACAACACAGGTCCCAGGAGCTACAGGGAACTTTGTTTTTATAAAAGATGCAGTCTTTGAAAAACCTGACACATCTATACTTCCTTTCCCAACTTACTTTGTGCCGGAAGATGAAGATTCAGATGATATGAAACCTTTGGTTGCTGATCTTGGGGATGTGGATCCATTTATGGTTACTGATTAA
Protein sequence

>MS.gene001687.t1

MLAFSRGVVSRLRQFPVAPLPRFFSSDVETIRTIEAKPSVMTPNSQRTGVIAVKCGMTAHWDKWGARIPISILWVDDNIVSQVKTPEKEGYCSLQIGCGQKKEKHLTKPEVGHFRAQGVPMKRKLQEFRVTEDALLPVGTSLNVRHFVPGQYVDITGITKGKGFQGVMKKYGFHGGSASHGNSKAHRTMGSTGILGPCKVFKGRKMPGRMGGKQRTVKNVWVYKIDPARNLMWVKGQVPGATGNFVFIKDAVFEKPDTSILPFPTYFVPEDEDSDDMKPLVADLGDVDPFMVTD