AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60589

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60589.t1 MTR_1g093060 98.299 294 5 0 1 294 54 347 0.0 597
MS.gene60589.t1 MTR_6g006970 92.857 294 21 0 1 294 1 294 0.0 572
MS.gene60589.t1 MTR_2g090370 93.269 104 7 0 189 292 1 104 2.40e-68 213
MS.gene60589.t1 MTR_3g118210 41.063 207 116 6 49 251 58 262 6.36e-43 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60589.t1 AT3G17465 66.975 324 77 4 1 294 1 324 1.77e-147 417
MS.gene60589.t1 AT2G43030 38.835 206 122 4 49 251 57 261 4.59e-39 138

Find 85 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGGATGTGGATCCATTTA+TGG 0.158517 1.2:-69300322 MS.gene60589:CDS
ACACCAGTTCTTTGTGAATT+TGG 0.165812 1.2:+69303051 None:intergenic
TGACTGCTCATTGGGATAAA+TGG 0.166624 1.2:-69303008 MS.gene60589:CDS
CTCACTGTTCAGGTCTTTAA+AGG 0.228704 1.2:-69301119 MS.gene60589:intron
GAATTCCAATTTCTATCCTT+TGG 0.239774 1.2:-69302978 MS.gene60589:CDS
CTTTAATCAGTAACCATAAA+TGG 0.243016 1.2:+69300309 None:intergenic
AAACCTTTGGTTGCTGATCT+TGG 0.318983 1.2:-69300342 MS.gene60589:CDS
TCTACCCAAAGGATAGAAAT+TGG 0.334271 1.2:+69302973 None:intergenic
AACCTTTGGTTGCTGATCTT+GGG 0.341776 1.2:-69300341 MS.gene60589:CDS
ACCCACATCAAATTTCTTGC+TGG 0.343726 1.2:+69301026 None:intergenic
GTTCAGATGTGGAGACTATA+AGG 0.352256 1.2:-69303104 MS.gene60589:CDS
CATACTGCCCGGGAACAAAA+TGG 0.352881 1.2:+69302169 None:intergenic
CAGGGTGTAATGAAGAAGTA+TGG 0.352968 1.2:-69301347 MS.gene60589:CDS
GCCCTAAAATGACCCACTTC+AGG 0.379527 1.2:+69302279 None:intergenic
TCATCTGAATCTTCATCTTC+CGG 0.389387 1.2:+69300369 None:intergenic
TTCAGATGATATGAAACCTT+TGG 0.395479 1.2:-69300355 MS.gene60589:CDS
AATTCCAATTTCTATCCTTT+GGG 0.400773 1.2:-69302977 MS.gene60589:CDS
ATGAAGAAGTATGGATTTCA+TGG 0.403214 1.2:-69301338 MS.gene60589:CDS
GACTGCTCATTGGGATAAAT+GGG 0.404613 1.2:-69303007 MS.gene60589:CDS
GGTTGCTGATCTTGGGGATG+TGG 0.407346 1.2:-69300334 MS.gene60589:CDS
GAACAATAGCCTTCCTTCTC+AGG 0.410835 1.2:+69302922 None:intergenic
TAGAAAGATGCCAGGGAGAA+TGG 0.416986 1.2:-69301096 MS.gene60589:CDS
TGCTTCCCACCCATTCTCCC+TGG 0.421120 1.2:+69301086 None:intergenic
ACAAAGTTCCCTGTAGCTCC+TGG 0.431609 1.2:+69300459 None:intergenic
AGGAATACTTGGTCCTTGTA+AGG 0.431878 1.2:-69301264 MS.gene60589:intron
GATTGGTTGTGGACAGAAGA+AGG 0.441589 1.2:-69302319 MS.gene60589:CDS
ACACAGGTCCCAGGAGCTAC+AGG 0.448282 1.2:-69300468 MS.gene60589:intron
ACCTTTGGTTGCTGATCTTG+GGG 0.448994 1.2:-69300340 MS.gene60589:CDS
ATGTGGAATGACTGCTCATT+GGG 0.453159 1.2:-69303016 MS.gene60589:CDS
CATAGAACCATGGGTTCTAC+AGG 0.456921 1.2:-69301284 MS.gene60589:CDS
TCTTCCGGCACAAAGTAAGT+TGG 0.469195 1.2:+69300384 None:intergenic
AGATGACAACATTGTCTCTC+AGG 0.475183 1.2:-69302953 MS.gene60589:CDS
ACACCAAATTCACAAAGAAC+TGG 0.478282 1.2:-69303054 MS.gene60589:CDS
CTTCCGGCACAAAGTAAGTT+GGG 0.483444 1.2:+69300385 None:intergenic
AGAAAGATGCCAGGGAGAAT+GGG 0.487342 1.2:-69301095 MS.gene60589:CDS
AGAAATTTGATGTGGGTGAA+AGG 0.489286 1.2:-69301020 MS.gene60589:CDS
GGACTCCATCCAACCTTACA+AGG 0.491579 1.2:+69301251 None:intergenic
AATCGCGGCAGTGGTGCCAC+GGG 0.495730 1.2:+69303132 None:intergenic
ACAGGAATCACCAAAGGAAA+AGG 0.499760 1.2:-69302138 MS.gene60589:CDS
AATGTGGAATGACTGCTCAT+TGG 0.503569 1.2:-69303017 MS.gene60589:CDS
ACATTAAGTGATGTACCAAC+AGG 0.507292 1.2:+69302195 None:intergenic
TATGTGCTTTCGAATTCCCA+TGG 0.513084 1.2:+69301303 None:intergenic
TCTTGAAGCTTCCTCTTCAT+CGG 0.518555 1.2:+69302246 None:intergenic
GAATTTGGTGTCATCACACT+AGG 0.526237 1.2:+69303066 None:intergenic
CTCAAGGAGTTCCGATGAAG+AGG 0.527266 1.2:-69302257 MS.gene60589:CDS
TTTGATGTGGGTGAAAGGCC+AGG 0.528326 1.2:-69301015 MS.gene60589:intron
AAAGGAAGTATAGATGTGTC+AGG 0.528945 1.2:+69300408 None:intergenic
GGAAGGCTATTGTTCTCTCC+AGG 0.530451 1.2:-69302914 MS.gene60589:intron
ATGGGTTCTACAGGAATACT+TGG 0.533632 1.2:-69301275 MS.gene60589:CDS
TGTGATGTCAACATACTGCC+CGG 0.537181 1.2:+69302158 None:intergenic
AAATCGCGGCAGTGGTGCCA+CGG 0.542479 1.2:+69303131 None:intergenic
GAGGATGCACTTCTTCCTGT+TGG 0.552055 1.2:-69302210 MS.gene60589:CDS
TTTCCCAACTTACTTTGTGC+CGG 0.552953 1.2:-69300388 MS.gene60589:CDS
CAAAGTTCCCTGTAGCTCCT+GGG 0.553971 1.2:+69300460 None:intergenic
CATGGAGGTTCAGCATCCCA+TGG 0.557351 1.2:-69301320 MS.gene60589:CDS
ACACTTGACAAAGCCTGAAG+TGG 0.560843 1.2:-69302292 MS.gene60589:CDS
TTTAAAGGTAGAAAGATGCC+AGG 0.564006 1.2:-69301104 MS.gene60589:CDS
TCAGGTCAAAACTCCTGAGA+AGG 0.567631 1.2:-69302935 MS.gene60589:CDS
ATGTGCTTTCGAATTCCCAT+GGG 0.571228 1.2:+69301304 None:intergenic
TCAAGAGTTTCGAGTGACAG+AGG 0.581725 1.2:-69302229 MS.gene60589:CDS
GTGTGCACAACACAGGTCCC+AGG 0.582644 1.2:-69300477 MS.gene60589:intron
ATGGAGGTTCAGCATCCCAT+GGG 0.591854 1.2:-69301319 MS.gene60589:CDS
GGGCAGTATGTTGACATCAC+AGG 0.594209 1.2:-69302156 MS.gene60589:CDS
AAGATGCCAGGGAGAATGGG+TGG 0.599236 1.2:-69301092 MS.gene60589:CDS
AAGTATTCCTGTAGAACCCA+TGG 0.604216 1.2:+69301277 None:intergenic
TCGAAAGCACATAGAACCAT+GGG 0.605881 1.2:-69301293 MS.gene60589:CDS
GGCACAAAGTAAGTTGGGAA+AGG 0.607369 1.2:+69300390 None:intergenic
ACCTCTTGAAAACGCCAGCA+TGG 0.609546 1.2:+69303179 None:intergenic
CACTTGACAAAGCCTGAAGT+GGG 0.609676 1.2:-69302291 MS.gene60589:CDS
GTCAAAACTCCTGAGAAGGA+AGG 0.621009 1.2:-69302931 MS.gene60589:CDS
ATCCAGCAAGAAATTTGATG+TGG 0.622717 1.2:-69301028 MS.gene60589:CDS
TTCGAAAGCACATAGAACCA+TGG 0.623227 1.2:-69301294 MS.gene60589:CDS
CACAGGTCCCAGGAGCTACA+GGG 0.625211 1.2:-69300467 MS.gene60589:intron
GTGATGTCAACATACTGCCC+GGG 0.631463 1.2:+69302159 None:intergenic
AGATGCCAGGGAGAATGGGT+GGG 0.644345 1.2:-69301091 MS.gene60589:CDS
GGTGTTATAGCTGTAAAATG+TGG 0.647527 1.2:-69303033 MS.gene60589:CDS
TCCCCAAGATCAGCAACCAA+AGG 0.647605 1.2:+69300339 None:intergenic
GACATCACAGGAATCACCAA+AGG 0.651363 1.2:-69302144 MS.gene60589:CDS
CAATGTTGTCATCTACCCAA+AGG 0.657641 1.2:+69302962 None:intergenic
TTAAAGGTAGAAAGATGCCA+GGG 0.664670 1.2:-69301103 MS.gene60589:CDS
TCCAGCAAGAAATTTGATGT+GGG 0.677547 1.2:-69301027 MS.gene60589:CDS
AAGAAGTATGGATTTCATGG+AGG 0.684184 1.2:-69301335 MS.gene60589:CDS
ATCGCGGCAGTGGTGCCACG+GGG 0.726950 1.2:+69303133 None:intergenic
TCGTCTTCGACAATTCCCCG+TGG 0.741512 1.2:-69303148 MS.gene60589:CDS
ACTGCTCATTGGGATAAATG+GGG 0.750912 1.2:-69303006 MS.gene60589:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTAAATTTGTATTTGT+AGG - chr1.2:69301586-69301605 MS.gene60589:intron 10.0%
!! AATGTTTATAATGAATAATG+CGG + chr1.2:69300726-69300745 None:intergenic 15.0%
!! CTACTAAATTAAATACAAAA+TGG + chr1.2:69302917-69302936 None:intergenic 15.0%
!! GACAGAAATTATTATTTATA+TGG + chr1.2:69301427-69301446 None:intergenic 15.0%
!! GTTTATAATGAGTTATTAAA+TGG - chr1.2:69301033-69301052 MS.gene60589:CDS 15.0%
!! TTGAACAAAACAATAAATAA+TGG + chr1.2:69301776-69301795 None:intergenic 15.0%
!! TTGATTACATTAGTTATATT+AGG - chr1.2:69301655-69301674 MS.gene60589:intron 15.0%
!!! TTTTTATGTACAAAATTTCT+TGG + chr1.2:69302852-69302871 None:intergenic 15.0%
!! AAAGAACAGTAAAAAATGTA+TGG - chr1.2:69302424-69302443 MS.gene60589:intron 20.0%
!! AAGAACAGTAAAAAATGTAT+GGG - chr1.2:69302425-69302444 MS.gene60589:intron 20.0%
!! ACAAAAAAACAATCATCTTA+GGG + chr1.2:69301626-69301645 None:intergenic 20.0%
!! CAAATTCAATCATGTATTAT+AGG - chr1.2:69301750-69301769 MS.gene60589:intron 20.0%
!! GCAGAAAAATAAACAATAAA+TGG + chr1.2:69301990-69302009 None:intergenic 20.0%
!! TACAAAAAAACAATCATCTT+AGG + chr1.2:69301627-69301646 None:intergenic 20.0%
!! TATATATAGAAGACATATCT+AGG + chr1.2:69301090-69301109 None:intergenic 20.0%
!! TGTCAACTTATTATATCATT+AGG + chr1.2:69301732-69301751 None:intergenic 20.0%
!! TTATTATATCATTAGGATTC+AGG + chr1.2:69301725-69301744 None:intergenic 20.0%
!! TTCACATTAATTTATGAACT+TGG - chr1.2:69302519-69302538 MS.gene60589:intron 20.0%
!!! AAATTATATTTTCTGTTTCC+AGG - chr1.2:69302122-69302141 MS.gene60589:intron 20.0%
!!! AAGATGATTGTTTTTTTGTA+AGG - chr1.2:69301627-69301646 MS.gene60589:intron 20.0%
!!! AATTATATTTTCTGTTTCCA+GGG - chr1.2:69302123-69302142 MS.gene60589:intron 20.0%
!!! AGATGATTGTTTTTTTGTAA+GGG - chr1.2:69301628-69301647 MS.gene60589:intron 20.0%
!!! TACACGTTTTTTTTTTTGAA+TGG - chr1.2:69302078-69302097 MS.gene60589:intron 20.0%
! AACATGTAAAAACAATTACC+TGG + chr1.2:69302494-69302513 None:intergenic 25.0%
! AACTAAGAATACTAATCTGA+AGG + chr1.2:69302261-69302280 None:intergenic 25.0%
! AATTCCAATTTCTATCCTTT+GGG - chr1.2:69300511-69300530 MS.gene60589:intron 25.0%
! ACAGTAAAAAAAGCAGAATA+TGG - chr1.2:69300977-69300996 MS.gene60589:intron 25.0%
! ATTTACTTCTAGACAATCAT+TGG - chr1.2:69301817-69301836 MS.gene60589:intron 25.0%
! TAGCTCAATTATTCATTAAC+TGG - chr1.2:69302934-69302953 MS.gene60589:CDS 25.0%
! TTGAATTTCACCAATCTAAT+CGG + chr1.2:69301012-69301031 None:intergenic 25.0%
!! ATATGAAAGTATGTAATACG+AGG + chr1.2:69301398-69301417 None:intergenic 25.0%
!! GGCAAAAATATTTGAAATCT+CGG - chr1.2:69302955-69302974 MS.gene60589:CDS 25.0%
!! TATGAAAGTATGTAATACGA+GGG + chr1.2:69301397-69301416 None:intergenic 25.0%
!!! ATGCTTTGTTTTAGTTAGAT+TGG - chr1.2:69301152-69301171 MS.gene60589:intron 25.0%
AAAAAAAACGTGTAACTGCA+AGG + chr1.2:69302072-69302091 None:intergenic 30.0%
AATCCACATGAAACTCATTA+GGG + chr1.2:69300636-69300655 None:intergenic 30.0%
AATGCTTATTCCTCTTTGTA+TGG - chr1.2:69302989-69303008 MS.gene60589:CDS 30.0%
ACATCTGAACTAAAAAATCG+CGG + chr1.2:69300374-69300393 None:intergenic 30.0%
ACTTGATCCTTAACATAGTA+TGG - chr1.2:69300923-69300942 MS.gene60589:intron 30.0%
ATTATCGCCATACTATGTTA+AGG + chr1.2:69300933-69300952 None:intergenic 30.0%
ATTTATGTTTCTCACTGTTC+AGG - chr1.2:69302359-69302378 MS.gene60589:intron 30.0%
CGAAAATTTGTGTAGTTGAT+TGG + chr1.2:69300762-69300781 None:intergenic 30.0%
CGTTTGTTTATTGACATGAT+TGG - chr1.2:69301544-69301563 MS.gene60589:intron 30.0%
GAATTCCAATTTCTATCCTT+TGG - chr1.2:69300510-69300529 MS.gene60589:intron 30.0%
GATTGCATAGATACAAATTC+TGG - chr1.2:69300880-69300899 MS.gene60589:intron 30.0%
GGATGAATCAATCATATTCA+TGG - chr1.2:69302625-69302644 MS.gene60589:intron 30.0%
TAATATCATCAAGAGAACGA+TGG - chr1.2:69302881-69302900 MS.gene60589:intron 30.0%
TAATCCACATGAAACTCATT+AGG + chr1.2:69300637-69300656 None:intergenic 30.0%
TACATGTTTATGCTTGTATG+TGG - chr1.2:69301471-69301490 MS.gene60589:intron 30.0%
TTCAGATGATATGAAACCTT+TGG - chr1.2:69303133-69303152 MS.gene60589:CDS 30.0%
TTGAAATCTCGGATTTATCA+CGG - chr1.2:69302966-69302985 MS.gene60589:CDS 30.0%
TTGTCATTCATGTATGATTG+CGG - chr1.2:69302022-69302041 MS.gene60589:intron 30.0%
TTGTGTAGTTGATTGGATTT+CGG + chr1.2:69300755-69300774 None:intergenic 30.0%
! ATGAAGAAGTATGGATTTCA+TGG - chr1.2:69302150-69302169 MS.gene60589:CDS 30.0%
!! AATCACTTGATTTGTGTATG+AGG + chr1.2:69302574-69302593 None:intergenic 30.0%
!! CAAAAGGGTAAGTTTTAACA+TGG + chr1.2:69302661-69302680 None:intergenic 30.0%
!!! TGTTTTAGTTAGATTGGTTG+TGG - chr1.2:69301158-69301177 MS.gene60589:intron 30.0%
!!! TTTTTTGAATGGCAGTTACA+CGG - chr1.2:69302089-69302108 MS.gene60589:intron 30.0%
AATCCGGAGTTCAATCAAAA+GGG + chr1.2:69302676-69302695 None:intergenic 35.0%
ACACCAAATTCACAAAGAAC+TGG - chr1.2:69300434-69300453 MS.gene60589:CDS 35.0%
ACACCAGTTCTTTGTGAATT+TGG + chr1.2:69300440-69300459 None:intergenic 35.0%
ACATTAAGTGATGTACCAAC+AGG + chr1.2:69301296-69301315 None:intergenic 35.0%
AGAAATTTGATGTGGGTGAA+AGG - chr1.2:69302468-69302487 MS.gene60589:intron 35.0%
ATCCAGCAAGAAATTTGATG+TGG - chr1.2:69302460-69302479 MS.gene60589:intron 35.0%
ATTTATGAACTTGGAGCTCT+AGG - chr1.2:69302528-69302547 MS.gene60589:intron 35.0%
CATAGTATGGCGATAATATC+TGG - chr1.2:69300936-69300955 MS.gene60589:intron 35.0%
GCGATAATATCTGGTTAGAT+TGG - chr1.2:69300945-69300964 MS.gene60589:intron 35.0%
TAATCCGGAGTTCAATCAAA+AGG + chr1.2:69302677-69302696 None:intergenic 35.0%
TATCCCTAATGAGTTTCATG+TGG - chr1.2:69300630-69300649 MS.gene60589:intron 35.0%
TCATCTGAATCTTCATCTTC+CGG + chr1.2:69303122-69303141 None:intergenic 35.0%
TCATTAATGACCAAGTGTCT+TGG + chr1.2:69302747-69302766 None:intergenic 35.0%
TCCAGCAAGAAATTTGATGT+GGG - chr1.2:69302461-69302480 MS.gene60589:intron 35.0%
TCTACCCAAAGGATAGAAAT+TGG + chr1.2:69300518-69300537 None:intergenic 35.0%
TGCGGAGTAACTAAACTAAA+AGG + chr1.2:69300708-69300727 None:intergenic 35.0%
TGCTATTATATAGACACGTG+CGG + chr1.2:69301499-69301518 None:intergenic 35.0%
TTAAAGGTAGAAAGATGCCA+GGG - chr1.2:69302385-69302404 MS.gene60589:intron 35.0%
TTTAAAGGTAGAAAGATGCC+AGG - chr1.2:69302384-69302403 MS.gene60589:intron 35.0%
TTTATGAACTTGGAGCTCTA+GGG - chr1.2:69302529-69302548 MS.gene60589:intron 35.0%
! AAAGGAAGTATAGATGTGTC+AGG + chr1.2:69303083-69303102 None:intergenic 35.0%
! AAGAAGTATGGATTTCATGG+AGG - chr1.2:69302153-69302172 MS.gene60589:CDS 35.0%
! GGTGTTATAGCTGTAAAATG+TGG - chr1.2:69300455-69300474 MS.gene60589:CDS 35.0%
! TTTTTGAATGGCAGTTACAC+GGG - chr1.2:69302090-69302109 MS.gene60589:intron 35.0%
!! GGTTGATGAGTTTGAGTTAA+AGG - chr1.2:69302604-69302623 MS.gene60589:intron 35.0%
!! TTACCCTTTTGATTGAACTC+CGG - chr1.2:69302670-69302689 MS.gene60589:intron 35.0%
!!! GCGATTTTTTAGTTCAGATG+TGG - chr1.2:69300373-69300392 MS.gene60589:CDS 35.0%
AAACCTTTGGTTGCTGATCT+TGG - chr1.2:69303146-69303165 MS.gene60589:CDS 40.0%
AACCTTTGGTTGCTGATCTT+GGG - chr1.2:69303147-69303166 MS.gene60589:CDS 40.0%
AACTGCAAATGTGCAAATGG+TGG + chr1.2:69302796-69302815 None:intergenic 40.0%
AAGACATATCTAGGTACTCC+AGG + chr1.2:69301081-69301100 None:intergenic 40.0%
AAGTATTCCTGTAGAACCCA+TGG + chr1.2:69302214-69302233 None:intergenic 40.0%
AATCTTGCACGTACAAGTTC+AGG - chr1.2:69301867-69301886 MS.gene60589:intron 40.0%
AATGTGGAATGACTGCTCAT+TGG - chr1.2:69300471-69300490 MS.gene60589:intron 40.0%
ACAGGAATCACCAAAGGAAA+AGG - chr1.2:69301350-69301369 MS.gene60589:intron 40.0%
ACCCACATCAAATTTCTTGC+TGG + chr1.2:69302465-69302484 None:intergenic 40.0%
ACTGCAAATGTGCAAATGGT+GGG + chr1.2:69302795-69302814 None:intergenic 40.0%
ACTGCTCATTGGGATAAATG+GGG - chr1.2:69300482-69300501 MS.gene60589:intron 40.0%
AGATGACAACATTGTCTCTC+AGG - chr1.2:69300535-69300554 MS.gene60589:intron 40.0%
AGGAATACTTGGTCCTTGTA+AGG - chr1.2:69302224-69302243 MS.gene60589:CDS 40.0%
ATACTTGGTCCTTGTAAGGT+TGG - chr1.2:69302228-69302247 MS.gene60589:CDS 40.0%
ATGGGTTCTACAGGAATACT+TGG - chr1.2:69302213-69302232 MS.gene60589:CDS 40.0%
ATGTGCTTTCGAATTCCCAT+GGG + chr1.2:69302187-69302206 None:intergenic 40.0%
ATGTGGAATGACTGCTCATT+GGG - chr1.2:69300472-69300491 MS.gene60589:intron 40.0%
ATTCAGCTTAGAGTTACTCC+TGG - chr1.2:69301060-69301079 MS.gene60589:CDS 40.0%
CAATGTTGTCATCTACCCAA+AGG + chr1.2:69300529-69300548 None:intergenic 40.0%
CAGGGTGTAATGAAGAAGTA+TGG - chr1.2:69302141-69302160 MS.gene60589:CDS 40.0%
CATACTTCTTCATTACACCC+TGG + chr1.2:69302143-69302162 None:intergenic 40.0%
CCAATATCAACCAAGACACT+TGG - chr1.2:69302734-69302753 MS.gene60589:intron 40.0%
CCATTTGCACATTTGCAGTT+CGG - chr1.2:69302796-69302815 MS.gene60589:intron 40.0%
CTCACTGTTCAGGTCTTTAA+AGG - chr1.2:69302369-69302388 MS.gene60589:intron 40.0%
GACTGCTCATTGGGATAAAT+GGG - chr1.2:69300481-69300500 MS.gene60589:intron 40.0%
GATTGAACTCCGGATTACTA+GGG - chr1.2:69302680-69302699 MS.gene60589:intron 40.0%
GCTATTATATAGACACGTGC+GGG + chr1.2:69301498-69301517 None:intergenic 40.0%
GTGACAGTATCCGATTAGAT+TGG - chr1.2:69300999-69301018 MS.gene60589:intron 40.0%
GTTCAGATGTGGAGACTATA+AGG - chr1.2:69300384-69300403 MS.gene60589:CDS 40.0%
TATCATCAAGAGAACGATGG+TGG - chr1.2:69302884-69302903 MS.gene60589:intron 40.0%
TATGTGCTTTCGAATTCCCA+TGG + chr1.2:69302188-69302207 None:intergenic 40.0%
TCTTGAAGCTTCCTCTTCAT+CGG + chr1.2:69301245-69301264 None:intergenic 40.0%
TGACTGCTCATTGGGATAAA+TGG - chr1.2:69300480-69300499 MS.gene60589:intron 40.0%
TGATTGAACTCCGGATTACT+AGG - chr1.2:69302679-69302698 MS.gene60589:intron 40.0%
TGCGTTTCAAATTTCCAGAG+AGG + chr1.2:69301937-69301956 None:intergenic 40.0%
TTCAAATTTCCAGAGAGGTC+AGG + chr1.2:69301932-69301951 None:intergenic 40.0%
TTTCCCAACTTACTTTGTGC+CGG - chr1.2:69303100-69303119 MS.gene60589:CDS 40.0%
! AACGCAAAATGTTGAGTTGC+AGG - chr1.2:69301951-69301970 MS.gene60589:intron 40.0%
! CACCAAAGGAAAAGGTTTTC+AGG - chr1.2:69301358-69301377 MS.gene60589:intron 40.0%
! CACCTGAAAACCTTTTCCTT+TGG + chr1.2:69301363-69301382 None:intergenic 40.0%
! CCAAGTGTCTTGGTTGATAT+TGG + chr1.2:69302737-69302756 None:intergenic 40.0%
! TCGAAAGCACATAGAACCAT+GGG - chr1.2:69302195-69302214 MS.gene60589:CDS 40.0%
! TTATGAACTTGGAGCTCTAG+GGG - chr1.2:69302530-69302549 MS.gene60589:intron 40.0%
! TTCGAAAGCACATAGAACCA+TGG - chr1.2:69302194-69302213 MS.gene60589:CDS 40.0%
!! AATGTTCGCCATTTTGTTCC+CGG - chr1.2:69301311-69301330 MS.gene60589:CDS 40.0%
!! GAATTTGGTGTCATCACACT+AGG + chr1.2:69300425-69300444 None:intergenic 40.0%
!! TAGCAGTTGAGTTGAATGCA+TGG - chr1.2:69301514-69301533 MS.gene60589:intron 40.0%
ACACTTGACAAAGCCTGAAG+TGG - chr1.2:69301196-69301215 MS.gene60589:intron 45.0%
ACCTTTGGTTGCTGATCTTG+GGG - chr1.2:69303148-69303167 MS.gene60589:CDS 45.0%
AGAAAGATGCCAGGGAGAAT+GGG - chr1.2:69302393-69302412 MS.gene60589:intron 45.0%
AGAATGAAGCATAACGCACC+TGG + chr1.2:69300595-69300614 None:intergenic 45.0%
ATCAAGTGATTGAGCTCGAG+CGG - chr1.2:69302583-69302602 MS.gene60589:intron 45.0%
CACTTGACAAAGCCTGAAGT+GGG - chr1.2:69301197-69301216 MS.gene60589:intron 45.0%
CATAGAACCATGGGTTCTAC+AGG - chr1.2:69302204-69302223 MS.gene60589:CDS 45.0%
CCAGAGAGGTCAGGTAAAAT+GGG + chr1.2:69301923-69301942 None:intergenic 45.0%
CCGAACTGCAAATGTGCAAA+TGG + chr1.2:69302799-69302818 None:intergenic 45.0%
CTTCCGGCACAAAGTAAGTT+GGG + chr1.2:69303106-69303125 None:intergenic 45.0%
GAACAATAGCCTTCCTTCTC+AGG + chr1.2:69300569-69300588 None:intergenic 45.0%
GAACTAAAAAATCGCGGCAG+TGG + chr1.2:69300368-69300387 None:intergenic 45.0%
GACATCACAGGAATCACCAA+AGG - chr1.2:69301344-69301363 MS.gene60589:CDS 45.0%
GCACGTACAAGTTCAGGTAA+TGG - chr1.2:69301873-69301892 MS.gene60589:intron 45.0%
GTCAAAACTCCTGAGAAGGA+AGG - chr1.2:69300557-69300576 MS.gene60589:intron 45.0%
GTGCGTTATGCTTCATTCTC+AGG - chr1.2:69300596-69300615 MS.gene60589:intron 45.0%
GTTGTGCACACCATACAAAG+AGG + chr1.2:69303002-69303021 None:intergenic 45.0%
TAGAAAGATGCCAGGGAGAA+TGG - chr1.2:69302392-69302411 MS.gene60589:intron 45.0%
TCAGGTCAAAACTCCTGAGA+AGG - chr1.2:69300553-69300572 MS.gene60589:intron 45.0%
TCCAGAGAGGTCAGGTAAAA+TGG + chr1.2:69301924-69301943 None:intergenic 45.0%
TCTTCCGGCACAAAGTAAGT+TGG + chr1.2:69303107-69303126 None:intergenic 45.0%
TGGGGATGTGGATCCATTTA+TGG - chr1.2:69303166-69303185 MS.gene60589:CDS 45.0%
TGTGATGTCAACATACTGCC+CGG + chr1.2:69301333-69301352 None:intergenic 45.0%
TTGGTCCTTGTAAGGTTGGA+TGG - chr1.2:69302232-69302251 MS.gene60589:CDS 45.0%
TTGTATGGTGTGCACAACAC+AGG - chr1.2:69303004-69303023 MS.gene60589:CDS 45.0%
! GATTGGTTGTGGACAGAAGA+AGG - chr1.2:69301169-69301188 MS.gene60589:intron 45.0%
! GGCACAAAGTAAGTTGGGAA+AGG + chr1.2:69303101-69303120 None:intergenic 45.0%
! TACTAGGGCTCCTTCCTTTT+GGG - chr1.2:69302695-69302714 MS.gene60589:intron 45.0%
! TCAAGAGTTTCGAGTGACAG+AGG - chr1.2:69301259-69301278 MS.gene60589:intron 45.0%
! TTACTAGGGCTCCTTCCTTT+TGG - chr1.2:69302694-69302713 MS.gene60589:intron 45.0%
!! AGCCTGAAGTGGGTCATTTT+AGG - chr1.2:69301207-69301226 MS.gene60589:intron 45.0%
!! ATGTTCGCCATTTTGTTCCC+GGG - chr1.2:69301312-69301331 MS.gene60589:CDS 45.0%
!! GCCTGAAGTGGGTCATTTTA+GGG - chr1.2:69301208-69301227 MS.gene60589:intron 45.0%
!! TGATATTGGCCTTGACTCTC+CGG + chr1.2:69302723-69302742 None:intergenic 45.0%
ACAAAGTTCCCTGTAGCTCC+TGG + chr1.2:69303032-69303051 None:intergenic 50.0%
AGGAAGGAGCCCTAGTAATC+CGG + chr1.2:69302692-69302711 None:intergenic 50.0%
ATGGAGGTTCAGCATCCCAT+GGG - chr1.2:69302169-69302188 MS.gene60589:CDS 50.0%
CAAAGTTCCCTGTAGCTCCT+GGG + chr1.2:69303031-69303050 None:intergenic 50.0%
CATACTGCCCGGGAACAAAA+TGG + chr1.2:69301322-69301341 None:intergenic 50.0%
CTCAAGGAGTTCCGATGAAG+AGG - chr1.2:69301231-69301250 MS.gene60589:intron 50.0%
GAGGATGCACTTCTTCCTGT+TGG - chr1.2:69301278-69301297 MS.gene60589:CDS 50.0%
GCCCTAAAATGACCCACTTC+AGG + chr1.2:69301212-69301231 None:intergenic 50.0%
GGAAGGCTATTGTTCTCTCC+AGG - chr1.2:69300574-69300593 MS.gene60589:intron 50.0%
GGACTCCATCCAACCTTACA+AGG + chr1.2:69302240-69302259 None:intergenic 50.0%
GGGCAGTATGTTGACATCAC+AGG - chr1.2:69301332-69301351 MS.gene60589:CDS 50.0%
GTGATGTCAACATACTGCCC+GGG + chr1.2:69301332-69301351 None:intergenic 50.0%
TCCCCAAGATCAGCAACCAA+AGG + chr1.2:69303152-69303171 None:intergenic 50.0%
TTGACTCTCCGGTTCCCAAA+AGG + chr1.2:69302712-69302731 None:intergenic 50.0%
TTTGATGTGGGTGAAAGGCC+AGG - chr1.2:69302473-69302492 MS.gene60589:intron 50.0%
! CCCATTTTACCTGACCTCTC+TGG - chr1.2:69301920-69301939 MS.gene60589:intron 50.0%
! GGATTCAGGACTCTGAAGAG+TGG + chr1.2:69301711-69301730 None:intergenic 50.0%
! GTTGAGTTGCAGGCGTTCTA+GGG - chr1.2:69301961-69301980 MS.gene60589:intron 50.0%
! TGTTGAGTTGCAGGCGTTCT+AGG - chr1.2:69301960-69301979 MS.gene60589:intron 50.0%
! TTGAGTTGCAGGCGTTCTAG+GGG - chr1.2:69301962-69301981 MS.gene60589:intron 50.0%
!! TTTTGGGAACCGGAGAGTCA+AGG - chr1.2:69302711-69302730 MS.gene60589:intron 50.0%
!!! GTGGGTCATTTTAGGGCTCA+AGG - chr1.2:69301215-69301234 MS.gene60589:intron 50.0%
AAGATGCCAGGGAGAATGGG+TGG - chr1.2:69302396-69302415 MS.gene60589:intron 55.0%
AGATGCCAGGGAGAATGGGT+GGG - chr1.2:69302397-69302416 MS.gene60589:intron 55.0%
CATGGAGGTTCAGCATCCCA+TGG - chr1.2:69302168-69302187 MS.gene60589:CDS 55.0%
CTCTCCGGTTCCCAAAAGGA+AGG + chr1.2:69302708-69302727 None:intergenic 55.0%
GGTTGCTGATCTTGGGGATG+TGG - chr1.2:69303154-69303173 MS.gene60589:CDS 55.0%
TCGTCTTCGACAATTCCCCG+TGG - chr1.2:69300340-69300359 MS.gene60589:CDS 55.0%
! TGAGTTGCAGGCGTTCTAGG+GGG - chr1.2:69301963-69301982 MS.gene60589:intron 55.0%
!! GCTGGCGTTTTCAAGAGGTG+TGG - chr1.2:69300313-69300332 MS.gene60589:CDS 55.0%
!! GGCTCCTTCCTTTTGGGAAC+CGG - chr1.2:69302701-69302720 MS.gene60589:intron 55.0%
ACACAGGTCCCAGGAGCTAC+AGG - chr1.2:69303020-69303039 MS.gene60589:CDS 60.0%
CACAGGTCCCAGGAGCTACA+GGG - chr1.2:69303021-69303040 MS.gene60589:CDS 60.0%
GTGTGCACAACACAGGTCCC+AGG - chr1.2:69303011-69303030 MS.gene60589:CDS 60.0%
TGCTTCCCACCCATTCTCCC+TGG + chr1.2:69302405-69302424 None:intergenic 60.0%
! AAATCGCGGCAGTGGTGCCA+CGG + chr1.2:69300360-69300379 None:intergenic 60.0%
!! GAGTTGCAGGCGTTCTAGGG+GGG - chr1.2:69301964-69301983 MS.gene60589:intron 60.0%
!! AATCGCGGCAGTGGTGCCAC+GGG + chr1.2:69300359-69300378 None:intergenic 65.0%
!! ATCGCGGCAGTGGTGCCACG+GGG + chr1.2:69300358-69300377 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 69300311 69303199 69300311 ID=MS.gene60589
chr1.2 mRNA 69300311 69303199 69300311 ID=MS.gene60589.t1;Parent=MS.gene60589
chr1.2 exon 69302915 69303199 69302915 ID=MS.gene60589.t1.exon1;Parent=MS.gene60589.t1
chr1.2 CDS 69302915 69303199 69302915 ID=cds.MS.gene60589.t1;Parent=MS.gene60589.t1
chr1.2 exon 69302131 69302340 69302131 ID=MS.gene60589.t1.exon2;Parent=MS.gene60589.t1
chr1.2 CDS 69302131 69302340 69302131 ID=cds.MS.gene60589.t1;Parent=MS.gene60589.t1
chr1.2 exon 69301265 69301366 69301265 ID=MS.gene60589.t1.exon3;Parent=MS.gene60589.t1
chr1.2 CDS 69301265 69301366 69301265 ID=cds.MS.gene60589.t1;Parent=MS.gene60589.t1
chr1.2 exon 69301016 69301129 69301016 ID=MS.gene60589.t1.exon4;Parent=MS.gene60589.t1
chr1.2 CDS 69301016 69301129 69301016 ID=cds.MS.gene60589.t1;Parent=MS.gene60589.t1
chr1.2 exon 69300311 69300484 69300311 ID=MS.gene60589.t1.exon5;Parent=MS.gene60589.t1
chr1.2 CDS 69300311 69300484 69300311 ID=cds.MS.gene60589.t1;Parent=MS.gene60589.t1
Gene Sequence

>MS.gene60589

ATGCTGGCGTTTTCAAGAGGTGTGGTTTCTCGTCTTCGACAATTCCCCGTGGCACCACTGCCGCGATTTTTTAGTTCAGATGTGGAGACTATAAGGACGATTGAAGCAAAACCTAGTGTGATGACACCAAATTCACAAAGAACTGGTGTTATAGCTGTAAAATGTGGAATGACTGCTCATTGGGATAAATGGGGTGCTAGAATTCCAATTTCTATCCTTTGGGTAGATGACAACATTGTCTCTCAGGTCAAAACTCCTGAGAAGGAAGGCTATTGTTCTCTCCAGGTGCGTTATGCTTCATTCTCAGGATCAAATTCAATATCCCTAATGAGTTTCATGTGGATTAACTCAGTTCAGAGTTACTTATTGAATAACTTAAGTTACTGCGACAATACCTTTTAGTTTAGTTACTCCGCATTATTCATTATAAACATTGAATTTCCGAAATCCAATCAACTACACAAATTTTCGAAAGAAATTAAAACTATTTGAAAGTTCATTAAAGAACTTCAAATGAACGTATAGTTATTAATTTTATTTTTTTAAATGCATGTAAACATCGTCTGCTAGATTGCATAGATACAAATTCTGGAATTATATGAAACTATGTGTACTTGATCCTTAACATAGTATGGCGATAATATCTGGTTAGATTGGTGAAATAATACAGTAAAAAAAGCAGAATATGGTGACAGTATCCGATTAGATTGGTGAAATTCAATGTTTATAATGAGTTATTAAATGGAATAATTCAGCTTAGAGTTACTCCTGGAGTACCTAGATATGTCTTCTATATATATGTATATAAAGTTAAATTTGTATCTAATTATAGACATGTTTGATGCTTTGTTTTAGTTAGATTGGTTGTGGACAGAAGAAGGAAAAACACTTGACAAAGCCTGAAGTGGGTCATTTTAGGGCTCAAGGAGTTCCGATGAAGAGGAAGCTTCAAGAGTTTCGAGTGACAGAGGATGCACTTCTTCCTGTTGGTACATCACTTAATGTTCGCCATTTTGTTCCCGGGCAGTATGTTGACATCACAGGAATCACCAAAGGAAAAGGTTTTCAGGTGTTTTTCTTTTTCCCTCGTATTACATACTTTCATATTGTTTTCCATATAAATAATAATTTCTGTCTTACATTTGAGTATGTAGCATGTGTACATGTTTATGCTTGTATGTGGTCCCGCACGTGTCTATATAATAGCAGTTGAGTTGAATGCATGGTGTTCTTCGTTTGTTTATTGACATGATTGGTTATTATTTTGTCAAAGAATTTATTAAATTTGTATTTGTAGGATTAAAATGAATTACCCTAAGATGATTGTTTTTTTGTAAGGGCTAGTTGATTACATTAGTTATATTAGGATTTTAAGTGTTGCTAATAACAATAAGTTGCCACTCTTCAGAGTCCTGAATCCTAATGATATAATAAGTTGACAAATTCAATCATGTATTATAGGCCATTATTTATTGTTTTGTTCAATTGTTTTCTGTTTCACGATTTATTTACTTCTAGACAATCATTGGTGTTCATTAGTTTCAACTTTCAACTATAATCTTGCACGTACAAGTTCAGGTAATGGTGCAACTTTATTCTTTTCTCTCTTCCCATTTTACCTGACCTCTCTGGAAATTTGAAACGCAAAATGTTGAGTTGCAGGCGTTCTAGGGGGGCCATTTATTGTTTATTTTTCTGCTTCAAAATGTATTTGTCATTCATGTATGATTGCGGATACTTCTCTGTTTCATGTTGAAACCTTGCAGTTACACGTTTTTTTTTTTGAATGGCAGTTACACGGGAGTTAATTTAAATTATATTTTCTGTTTCCAGGGTGTAATGAAGAAGTATGGATTTCATGGAGGTTCAGCATCCCATGGGAATTCGAAAGCACATAGAACCATGGGTTCTACAGGAATACTTGGTCCTTGTAAGGTTGGATGGAGTCCTTCAGATTAGTATTCTTAGTTAATACAGCTTTCTCTATTTGTACGTATTGAAATCATGTTAGATTGACTGTCTGTATAAGTTTATTTTCTTTGCATTACATTTATGTTTCTCACTGTTCAGGTCTTTAAAGGTAGAAAGATGCCAGGGAGAATGGGTGGGAAGCAAAGAACAGTAAAAAATGTATGGGTCTACAAAATTGATCCAGCAAGAAATTTGATGTGGGTGAAAGGCCAGGTAATTGTTTTTACATGTTTATCATTCACATTAATTTATGAACTTGGAGCTCTAGGGGAGTAACTATCTTATATCACCTCATACACAAATCAAGTGATTGAGCTCGAGCGGTTGATGAGTTTGAGTTAAAGGATGAATCAATCATATTCATGGCTGAAACAATCCATGTTAAAACTTACCCTTTTGATTGAACTCCGGATTACTAGGGCTCCTTCCTTTTGGGAACCGGAGAGTCAAGGCCAATATCAACCAAGACACTTGGTCATTAATGATTTTAAATTTTTATTAAATTTGAATCCCACCATTTGCACATTTGCAGTTCGGCTAGTACAGAGACTTGTATGTTTGTGTGAGCCAAGAAATTTTGTACATAAAAAAATTCTGACTAATATCATCAAGAGAACGATGGTGGATTGAAACCATTTTGTATTTAATTTAGTAGCTCAATTATTCATTAACTGGCAAAAATATTTGAAATCTCGGATTTATCACGGAATGCTTATTCCTCTTTGTATGGTGTGCACAACACAGGTCCCAGGAGCTACAGGGAACTTTGTTTTTATAAAAGATGCAGTCTTTGAAAAACCTGACACATCTATACTTCCTTTCCCAACTTACTTTGTGCCGGAAGATGAAGATTCAGATGATATGAAACCTTTGGTTGCTGATCTTGGGGATGTGGATCCATTTATGGTTACTGATTAA
Protein sequence

>MS.gene60589.t1

MLAFSRGVVSRLRQFPVAPLPRFFSSDVETIRTIEAKPSVMTPNSQRTGVIAVKCGMTAHWDKWGARIPISILWVDDNIVSQVKTPEKEGYCSLQIGCGQKKEKHLTKPEVGHFRAQGVPMKRKLQEFRVTEDALLPVGTSLNVRHFVPGQYVDITGITKGKGFQGVMKKYGFHGGSASHGNSKAHRTMGSTGILGPCKVFKGRKMPGRMGGKQRTVKNVWVYKIDPARNLMWVKGQVPGATGNFVFIKDAVFEKPDTSILPFPTYFVPEDEDSDDMKPLVADLGDVDPFMVTD