Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24021.t1 | XP_013468395.1 | 97.4 | 421 | 10 | 1 | 1 | 420 | 1 | 421 | 6.90E-241 | 842.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24021.t1 | Q9SVU0 | 71.9 | 424 | 114 | 2 | 1 | 419 | 1 | 424 | 2.8e-182 | 639.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24021.t1 | A0A072VKW7 | 97.4 | 421 | 10 | 1 | 1 | 420 | 1 | 421 | 5.0e-241 | 842.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene24021 | MS.gene013322 | PPI |
| MS.gene24021 | MS.gene60245 | PPI |
| MS.gene24021 | MS.gene57685 | PPI |
| MS.gene24021 | MS.gene031178 | PPI |
| MS.gene24021 | MS.gene64265 | PPI |
| MS.gene24021 | MS.gene56048 | PPI |
| MS.gene24021 | MS.gene035805 | PPI |
| MS.gene24021 | MS.gene015953 | PPI |
| MS.gene24021 | MS.gene36879 | PPI |
| MS.gene24021 | MS.gene019484 | PPI |
| MS.gene24021 | MS.gene027681 | PPI |
| MS.gene24021 | MS.gene064951 | PPI |
| MS.gene24021 | MS.gene064542 | PPI |
| MS.gene24021 | MS.gene44331 | PPI |
| MS.gene24021 | MS.gene86173 | PPI |
| MS.gene24021 | MS.gene32792 | PPI |
| MS.gene24021 | MS.gene61665 | PPI |
| MS.gene24021 | MS.gene059136 | PPI |
| MS.gene24021 | MS.gene40340 | PPI |
| MS.gene24021 | MS.gene06418 | PPI |
| MS.gene24021 | MS.gene021474 | PPI |
| MS.gene24021 | MS.gene44332 | PPI |
| MS.gene24021 | MS.gene53594 | PPI |
| MS.gene24021 | MS.gene51580 | PPI |
| MS.gene24021 | MS.gene014685 | PPI |
| MS.gene24021 | MS.gene31616 | PPI |
| MS.gene24021 | MS.gene29205 | PPI |
| MS.gene24021 | MS.gene049295 | PPI |
| MS.gene24021 | MS.gene73119 | PPI |
| MS.gene24021 | MS.gene054944 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24021.t1 | MTR_1g069275 | 97.387 | 421 | 10 | 1 | 1 | 420 | 1 | 421 | 0.0 | 839 |
| MS.gene24021.t1 | MTR_7g099330 | 78.385 | 421 | 85 | 3 | 1 | 420 | 1 | 416 | 0.0 | 690 |
| MS.gene24021.t1 | MTR_1g046230 | 72.289 | 415 | 111 | 1 | 10 | 420 | 9 | 423 | 0.0 | 624 |
| MS.gene24021.t1 | MTR_7g099160 | 77.508 | 329 | 69 | 2 | 93 | 420 | 213 | 537 | 0.0 | 529 |
| MS.gene24021.t1 | MTR_1g008380 | 63.753 | 389 | 139 | 2 | 18 | 404 | 35 | 423 | 0.0 | 523 |
| MS.gene24021.t1 | MTR_3g109520 | 60.298 | 403 | 157 | 2 | 4 | 403 | 18 | 420 | 0.0 | 511 |
| MS.gene24021.t1 | MTR_6g086870 | 59.446 | 397 | 155 | 3 | 9 | 399 | 4 | 400 | 1.03e-178 | 504 |
| MS.gene24021.t1 | MTR_1g011630 | 59.162 | 382 | 154 | 1 | 18 | 399 | 9 | 388 | 1.83e-178 | 504 |
| MS.gene24021.t1 | MTR_7g107710 | 47.368 | 380 | 191 | 4 | 26 | 401 | 7 | 381 | 3.07e-123 | 362 |
| MS.gene24021.t1 | MTR_8g432640 | 47.733 | 375 | 187 | 4 | 26 | 396 | 7 | 376 | 1.48e-120 | 355 |
| MS.gene24021.t1 | MTR_7g107690 | 44.327 | 379 | 202 | 4 | 26 | 400 | 9 | 382 | 6.27e-120 | 354 |
| MS.gene24021.t1 | MTR_5g033260 | 46.842 | 380 | 187 | 5 | 26 | 398 | 7 | 378 | 6.99e-120 | 354 |
| MS.gene24021.t1 | MTR_3g088925 | 44.267 | 375 | 198 | 5 | 26 | 395 | 8 | 376 | 7.03e-117 | 347 |
| MS.gene24021.t1 | MTR_3g088945 | 44.000 | 375 | 199 | 5 | 26 | 395 | 8 | 376 | 5.30e-116 | 344 |
| MS.gene24021.t1 | MTR_3g088955 | 43.467 | 375 | 201 | 5 | 26 | 395 | 8 | 376 | 4.85e-114 | 340 |
| MS.gene24021.t1 | MTR_4g051642 | 45.067 | 375 | 194 | 6 | 26 | 395 | 8 | 375 | 1.29e-113 | 338 |
| MS.gene24021.t1 | MTR_3g096030 | 38.068 | 176 | 83 | 2 | 26 | 199 | 7 | 158 | 9.23e-37 | 132 |
| MS.gene24021.t1 | MTR_2g450510 | 28.632 | 234 | 129 | 8 | 27 | 223 | 7 | 239 | 1.43e-14 | 75.9 |
| MS.gene24021.t1 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 2.02e-14 | 73.6 |
| MS.gene24021.t1 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 2.70e-14 | 73.9 |
| MS.gene24021.t1 | MTR_3g437580 | 24.017 | 229 | 139 | 5 | 27 | 220 | 15 | 243 | 3.95e-14 | 73.6 |
| MS.gene24021.t1 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 4.35e-14 | 73.9 |
| MS.gene24021.t1 | MTR_3g437580 | 24.017 | 229 | 139 | 5 | 27 | 220 | 15 | 243 | 6.25e-14 | 73.6 |
| MS.gene24021.t1 | MTR_2g044140 | 26.757 | 370 | 207 | 14 | 27 | 336 | 16 | 381 | 4.14e-12 | 68.2 |
| MS.gene24021.t1 | MTR_2g043850 | 25.806 | 372 | 208 | 16 | 27 | 336 | 16 | 381 | 2.60e-11 | 65.9 |
| MS.gene24021.t1 | MTR_2g020630 | 25.417 | 240 | 138 | 9 | 27 | 227 | 16 | 253 | 3.82e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24021.t1 | AT4G28720 | 71.934 | 424 | 114 | 2 | 1 | 419 | 1 | 424 | 0.0 | 646 |
| MS.gene24021.t1 | AT1G04180 | 71.122 | 419 | 120 | 1 | 1 | 418 | 1 | 419 | 0.0 | 624 |
| MS.gene24021.t1 | AT1G04610 | 69.451 | 419 | 123 | 1 | 7 | 420 | 19 | 437 | 0.0 | 613 |
| MS.gene24021.t1 | AT5G43890 | 69.194 | 422 | 126 | 2 | 1 | 418 | 1 | 422 | 0.0 | 613 |
| MS.gene24021.t1 | AT2G33230 | 69.324 | 414 | 121 | 2 | 12 | 420 | 19 | 431 | 0.0 | 582 |
| MS.gene24021.t1 | AT5G25620 | 60.349 | 401 | 156 | 2 | 9 | 406 | 16 | 416 | 5.20e-179 | 506 |
| MS.gene24021.t1 | AT5G25620 | 60.606 | 396 | 153 | 2 | 9 | 401 | 16 | 411 | 4.51e-178 | 504 |
| MS.gene24021.t1 | AT5G11320 | 59.843 | 381 | 149 | 1 | 20 | 400 | 12 | 388 | 1.69e-172 | 489 |
| MS.gene24021.t1 | AT4G32540 | 55.335 | 403 | 174 | 3 | 16 | 417 | 12 | 409 | 6.33e-172 | 488 |
| MS.gene24021.t1 | AT4G13260 | 60.789 | 380 | 146 | 2 | 18 | 394 | 21 | 400 | 1.62e-171 | 486 |
| MS.gene24021.t1 | AT4G32540 | 54.988 | 411 | 176 | 5 | 10 | 417 | 7 | 411 | 2.88e-170 | 483 |
| MS.gene24021.t1 | AT5G11320 | 59.202 | 326 | 129 | 1 | 20 | 345 | 12 | 333 | 1.19e-142 | 411 |
| MS.gene24021.t1 | AT4G13260 | 61.379 | 290 | 110 | 1 | 18 | 305 | 21 | 310 | 8.84e-132 | 382 |
| MS.gene24021.t1 | AT1G48910 | 47.215 | 377 | 188 | 5 | 26 | 397 | 6 | 376 | 8.65e-126 | 369 |
| MS.gene24021.t1 | AT1G21430 | 48.396 | 374 | 181 | 5 | 26 | 394 | 10 | 376 | 3.06e-123 | 363 |
| MS.gene24021.t1 | AT4G13260 | 58.131 | 289 | 118 | 2 | 109 | 394 | 9 | 297 | 1.37e-114 | 338 |
| MS.gene24021.t1 | AT1G62560 | 27.230 | 213 | 122 | 6 | 27 | 208 | 15 | 225 | 2.33e-19 | 89.7 |
| MS.gene24021.t1 | AT1G62560 | 27.230 | 213 | 122 | 6 | 27 | 208 | 15 | 225 | 5.44e-19 | 89.4 |
| MS.gene24021.t1 | AT5G45180 | 26.136 | 352 | 192 | 14 | 27 | 336 | 15 | 340 | 1.75e-17 | 84.7 |
| MS.gene24021.t1 | AT1G65860 | 25.561 | 223 | 138 | 4 | 27 | 221 | 15 | 237 | 3.18e-16 | 80.9 |
| MS.gene24021.t1 | AT1G12160 | 26.636 | 214 | 124 | 6 | 27 | 209 | 14 | 225 | 1.14e-15 | 79.3 |
| MS.gene24021.t1 | AT1G62620 | 25.926 | 243 | 138 | 8 | 27 | 234 | 15 | 250 | 1.92e-15 | 78.2 |
| MS.gene24021.t1 | AT5G07800 | 26.147 | 218 | 125 | 5 | 27 | 208 | 18 | 235 | 2.06e-15 | 78.2 |
| MS.gene24021.t1 | AT1G63370 | 25.000 | 240 | 144 | 7 | 27 | 234 | 15 | 250 | 3.40e-15 | 76.6 |
| MS.gene24021.t1 | AT1G62620 | 25.417 | 240 | 143 | 7 | 27 | 234 | 15 | 250 | 4.34e-15 | 77.4 |
| MS.gene24021.t1 | AT1G63370 | 25.000 | 240 | 144 | 7 | 27 | 234 | 15 | 250 | 7.80e-15 | 76.6 |
| MS.gene24021.t1 | AT5G61290 | 26.267 | 217 | 126 | 5 | 27 | 209 | 18 | 234 | 1.55e-14 | 75.5 |
| MS.gene24021.t1 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.00e-14 | 74.7 |
| MS.gene24021.t1 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.39e-14 | 74.3 |
| MS.gene24021.t1 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.55e-14 | 74.7 |
| MS.gene24021.t1 | AT1G12200 | 25.743 | 202 | 117 | 4 | 41 | 209 | 30 | 231 | 5.40e-14 | 73.6 |
| MS.gene24021.t1 | AT1G12200 | 25.743 | 202 | 117 | 4 | 41 | 209 | 30 | 231 | 1.51e-13 | 72.8 |
| MS.gene24021.t1 | AT1G12130 | 23.451 | 226 | 142 | 5 | 41 | 235 | 29 | 254 | 5.69e-13 | 70.9 |
| MS.gene24021.t1 | AT1G62570 | 22.967 | 209 | 133 | 3 | 41 | 221 | 29 | 237 | 1.66e-12 | 69.3 |
| MS.gene24021.t1 | AT1G12140 | 22.857 | 210 | 133 | 3 | 41 | 221 | 29 | 238 | 2.11e-12 | 68.9 |
| MS.gene24021.t1 | AT1G12140 | 22.857 | 210 | 133 | 3 | 41 | 221 | 29 | 238 | 2.11e-12 | 68.9 |
Find 91 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTCTTTGTTGGATATTC+AGG | 0.151113 | 1.3:+48582793 | None:intergenic |
| GTTGTTGGTTGTGGTAATTC+TGG | 0.205623 | 1.3:-48582484 | MS.gene24021:CDS |
| GGTAGTTGACAGAATTGTTT+AGG | 0.250500 | 1.3:+48582841 | None:intergenic |
| CTCAAGATATTGCTCAAGTT+TGG | 0.269888 | 1.3:-48581265 | MS.gene24021:CDS |
| ATCGTAGCAATGTCCCTTCT+TGG | 0.282135 | 1.3:-48581767 | MS.gene24021:CDS |
| TAGAAAAGTATGGTCTTAAA+AGG | 0.282601 | 1.3:-48581980 | MS.gene24021:CDS |
| CAATTTGTGCAATTTAGAAT+TGG | 0.285416 | 1.3:+48581183 | None:intergenic |
| GCTAAATATGATGAGACTAA+TGG | 0.295504 | 1.3:-48582697 | MS.gene24021:CDS |
| GGTCCTGCTCCTACTATTAT+AGG | 0.301581 | 1.3:+48582988 | None:intergenic |
| CATGCTTGTGATTATAAATC+AGG | 0.314519 | 1.3:-48582535 | MS.gene24021:CDS |
| AAAGGGTAAGACCCCTGTTT+TGG | 0.316214 | 1.3:-48581925 | MS.gene24021:CDS |
| TTACTCAACCCTTCAATATC+AGG | 0.327104 | 1.3:+48582577 | None:intergenic |
| GGCTGTGTTGAACTTGTTAA+TGG | 0.328799 | 1.3:-48581831 | MS.gene24021:CDS |
| TTTATGAACTGTTTCTTTGT+TGG | 0.340000 | 1.3:+48582784 | None:intergenic |
| CAGTTCCTTTCCAACCATTA+GGG | 0.343203 | 1.3:+48581357 | None:intergenic |
| TAGTCTCACATCGTTGCATT+TGG | 0.343936 | 1.3:-48583020 | MS.gene24021:CDS |
| AAGGCCATTGGAGGGTCCTT+TGG | 0.345490 | 1.3:-48581961 | MS.gene24021:CDS |
| ACAGTTCCTTTCCAACCATT+AGG | 0.345992 | 1.3:+48581356 | None:intergenic |
| CAATGTCCCTTCTTGGCTTA+AGG | 0.348481 | 1.3:-48581760 | MS.gene24021:intron |
| TTCTATTGTTCTTCGTAGTT+CGG | 0.355397 | 1.3:-48582419 | MS.gene24021:intron |
| TGACTTTGGAAATCCATTCT+TGG | 0.361539 | 1.3:+48581385 | None:intergenic |
| GTAGTTGACAGAATTGTTTA+GGG | 0.362984 | 1.3:+48582842 | None:intergenic |
| CCAAAGTCACCATTCCCTAA+TGG | 0.366181 | 1.3:-48581371 | MS.gene24021:CDS |
| TTAGTAAACCTCTTAATTCC+AGG | 0.368388 | 1.3:+48581855 | None:intergenic |
| TCTTGTGGGAATGGAAAGTT+AGG | 0.369111 | 1.3:+48582820 | None:intergenic |
| CTGTTGGACTTTATGCTGTT+GGG | 0.378354 | 1.3:-48581337 | MS.gene24021:CDS |
| CATCGTTGCATTTGGGTAAA+CGG | 0.385627 | 1.3:-48583012 | MS.gene24021:CDS |
| AATTTGTGCAATTTAGAATT+GGG | 0.388229 | 1.3:+48581184 | None:intergenic |
| AAGGTGCCAATATCCAAAAC+AGG | 0.397457 | 1.3:+48581912 | None:intergenic |
| AGTCTCACATCGTTGCATTT+GGG | 0.410240 | 1.3:-48583019 | MS.gene24021:CDS |
| ATACTCAACTTCATTTCCAT+TGG | 0.414194 | 1.3:+48582651 | None:intergenic |
| TTTGGTAATATAGAAAAGTA+TGG | 0.417326 | 1.3:-48581990 | MS.gene24021:CDS |
| CTAAATATGATGAGACTAAT+GGG | 0.418507 | 1.3:-48582696 | MS.gene24021:CDS |
| TGATGAAATGGTTACCCGTT+TGG | 0.423835 | 1.3:-48582052 | MS.gene24021:CDS |
| AGGTCGCTTGTGGTTGCTAC+TGG | 0.431631 | 1.3:-48582622 | MS.gene24021:CDS |
| GATGCAGTTGTTCTTGCTAC+TGG | 0.439635 | 1.3:-48581792 | MS.gene24021:CDS |
| CCATTAGGGAATGGTGACTT+TGG | 0.441458 | 1.3:+48581371 | None:intergenic |
| GAACTACGAAGAACAATAGA+AGG | 0.454169 | 1.3:+48582421 | None:intergenic |
| ACAACAAGGAGTACCCTTCA+TGG | 0.462402 | 1.3:-48582935 | MS.gene24021:CDS |
| TCAGGAAATTCTTGTGGGAA+TGG | 0.465607 | 1.3:+48582811 | None:intergenic |
| ATGAAGTTGAGTATATTTGT+AGG | 0.466018 | 1.3:-48582642 | MS.gene24021:CDS |
| GATATTCAGGAAATTCTTGT+GGG | 0.470627 | 1.3:+48582806 | None:intergenic |
| ACTGTTGGACTTTATGCTGT+TGG | 0.470697 | 1.3:-48581338 | MS.gene24021:CDS |
| ATAGTAGGAGCAGGACCATC+AGG | 0.478379 | 1.3:-48582982 | MS.gene24021:CDS |
| TGGCAGTTATGATGATGAAA+TGG | 0.484448 | 1.3:-48582064 | MS.gene24021:CDS |
| TTTACAAAGAGAGGGCTCTC+TGG | 0.487445 | 1.3:-48581314 | MS.gene24021:CDS |
| AATCTTGTCAACTATCCAAA+CGG | 0.487573 | 1.3:+48582037 | None:intergenic |
| TGGGAAATCAACATTTGGAT+TGG | 0.487762 | 1.3:-48582084 | MS.gene24021:CDS |
| GGAATTAAGAGGTTTACTAA+TGG | 0.490761 | 1.3:-48581852 | MS.gene24021:CDS |
| ATCTTGTCAACTATCCAAAC+GGG | 0.505639 | 1.3:+48582038 | None:intergenic |
| TTGGAGCTTAAGAACACAAA+GGG | 0.508263 | 1.3:-48581942 | MS.gene24021:CDS |
| TTTCTCACCTTAAGCCAAGA+AGG | 0.510383 | 1.3:+48581753 | None:intergenic |
| GTAAACGGTCCTATAATAGT+AGG | 0.514321 | 1.3:-48582997 | MS.gene24021:CDS |
| TGATCTTTCAAGCACCATGA+AGG | 0.516098 | 1.3:+48582921 | None:intergenic |
| AAGTTGTGCCTGGAATTAAG+AGG | 0.518103 | 1.3:-48581863 | MS.gene24021:CDS |
| GGATATTCAGGAAATTCTTG+TGG | 0.520358 | 1.3:+48582805 | None:intergenic |
| CCTTTCCAACCATTAGGGAA+TGG | 0.520534 | 1.3:+48581362 | None:intergenic |
| ACCTTGGAGAAAATTAGATC+TGG | 0.532203 | 1.3:-48581894 | MS.gene24021:CDS |
| GTATGGTCTTAAAAGGCCAT+TGG | 0.535113 | 1.3:-48581973 | MS.gene24021:CDS |
| GTTGACAGAATTGTTTAGGG+AGG | 0.539880 | 1.3:+48582845 | None:intergenic |
| GCTGTTGGGTTTACAAAGAG+AGG | 0.540106 | 1.3:-48581323 | MS.gene24021:CDS |
| TTTGTGTTCTTAAGCTCCAA+AGG | 0.541522 | 1.3:+48581945 | None:intergenic |
| TGTGATTATAAATCAGGTGA+AGG | 0.541872 | 1.3:-48582529 | MS.gene24021:CDS |
| TGGTTGTGGTAATTCTGGAA+TGG | 0.541889 | 1.3:-48582479 | MS.gene24021:CDS |
| TTTCTTCTTCGTTATCGACT+AGG | 0.550794 | 1.3:+48583043 | None:intergenic |
| TGGTCTTAAAAGGCCATTGG+AGG | 0.553703 | 1.3:-48581970 | MS.gene24021:CDS |
| TGTGTTGTGCCTGATATTGA+AGG | 0.554440 | 1.3:-48582586 | MS.gene24021:CDS |
| GCTGCTGTTGCTAGACCTGA+TGG | 0.555064 | 1.3:+48582967 | None:intergenic |
| AATGGTTGGAAAGGAACTGT+TGG | 0.555476 | 1.3:-48581353 | MS.gene24021:CDS |
| AGTCACCATTCCCTAATGGT+TGG | 0.557817 | 1.3:-48581367 | MS.gene24021:CDS |
| GAACACACTCGTTGAATCGT+GGG | 0.559010 | 1.3:+48582725 | None:intergenic |
| CCATTCCCTAATGGTTGGAA+AGG | 0.559102 | 1.3:-48581362 | MS.gene24021:CDS |
| ATGATGAGACTAATGGGTTG+TGG | 0.559573 | 1.3:-48582690 | MS.gene24021:CDS |
| GGTCACATAAAAGTTGTGCC+TGG | 0.561332 | 1.3:-48581873 | MS.gene24021:CDS |
| CTGTTGGGTTTACAAAGAGA+GGG | 0.568492 | 1.3:-48581322 | MS.gene24021:CDS |
| TGAACACACTCGTTGAATCG+TGG | 0.570661 | 1.3:+48582724 | None:intergenic |
| GATCTTTCAAGCACCATGAA+GGG | 0.575566 | 1.3:+48582922 | None:intergenic |
| TTCTCACCTTAAGCCAAGAA+GGG | 0.584034 | 1.3:+48581754 | None:intergenic |
| GATTTCGAAGTGTTTCGCGT+AGG | 0.586522 | 1.3:+48582750 | None:intergenic |
| GAAGGGTTGAGTAAATTCAA+AGG | 0.589620 | 1.3:-48582568 | MS.gene24021:CDS |
| AGCTCCAAAGGACCCTCCAA+TGG | 0.594886 | 1.3:+48581957 | None:intergenic |
| GGTCCTATAATAGTAGGAGC+AGG | 0.599245 | 1.3:-48582991 | MS.gene24021:CDS |
| GTATATTTGTAGGTCGCTTG+TGG | 0.619170 | 1.3:-48582632 | MS.gene24021:CDS |
| AGAGTTGAGACAGTTTCCAA+TGG | 0.619735 | 1.3:-48582667 | MS.gene24021:CDS |
| AGGTGCCAATATCCAAAACA+GGG | 0.629075 | 1.3:+48581913 | None:intergenic |
| GTGTTGTGCCTGATATTGAA+GGG | 0.636186 | 1.3:-48582585 | MS.gene24021:CDS |
| GGTCTTAAAAGGCCATTGGA+GGG | 0.661835 | 1.3:-48581969 | MS.gene24021:CDS |
| TTTGGAGCTTAAGAACACAA+AGG | 0.673099 | 1.3:-48581943 | MS.gene24021:CDS |
| GCAGCATGTCTTAAACAACA+AGG | 0.727523 | 1.3:-48582949 | MS.gene24021:CDS |
| GGTGCCAATATCCAAAACAG+GGG | 0.738091 | 1.3:+48581914 | None:intergenic |
| CAAATTGCATAGCTTCACTG+TGG | 0.767067 | 1.3:-48582900 | MS.gene24021:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTGTACTAATTA+AGG | - | chr1.3:48582100-48582119 | MS.gene24021:CDS | 10.0% |
| !!! | GTTAATAACTAATCTTATTT+TGG | - | chr1.3:48582815-48582834 | MS.gene24021:CDS | 15.0% |
| !!! | AGTATTTTACTATTTTTGTG+TGG | - | chr1.3:48582791-48582810 | MS.gene24021:CDS | 20.0% |
| !!! | TTGTAATTTTGTTTATTTCG+AGG | - | chr1.3:48582035-48582054 | MS.gene24021:CDS | 20.0% |
| !!! | TTTGGTAATATAGAAAAGTA+TGG | - | chr1.3:48582266-48582285 | MS.gene24021:intron | 20.0% |
| ! | ATACTAATTCCATTTCCAAA+AGG | + | chr1.3:48582776-48582795 | None:intergenic | 25.0% |
| ! | ATATGATAAGACCCAAAAAA+AGG | + | chr1.3:48582672-48582691 | None:intergenic | 25.0% |
| ! | ATGAAGTTGAGTATATTTGT+AGG | - | chr1.3:48581614-48581633 | MS.gene24021:intron | 25.0% |
| ! | CTAAATATGATGAGACTAAT+GGG | - | chr1.3:48581560-48581579 | MS.gene24021:intron | 25.0% |
| ! | TACTAATTCCATTTCCAAAA+GGG | + | chr1.3:48582775-48582794 | None:intergenic | 25.0% |
| !! | AATTAAGGTTGTTGTGTTTT+TGG | - | chr1.3:48582115-48582134 | MS.gene24021:intron | 25.0% |
| !! | TAGAAAAGTATGGTCTTAAA+AGG | - | chr1.3:48582276-48582295 | MS.gene24021:intron | 25.0% |
| !! | TTTATGAACTGTTTCTTTGT+TGG | + | chr1.3:48581475-48581494 | None:intergenic | 25.0% |
| !!! | TGACAAGATTCTTTTGATTT+TGG | - | chr1.3:48582232-48582251 | MS.gene24021:intron | 25.0% |
| !!! | TGGTTTTGAAACCTTTTTTT+GGG | - | chr1.3:48582658-48582677 | MS.gene24021:CDS | 25.0% |
| !!! | TTGGTTTTGAAACCTTTTTT+TGG | - | chr1.3:48582657-48582676 | MS.gene24021:CDS | 25.0% |
| AATCTTGTCAACTATCCAAA+CGG | + | chr1.3:48582222-48582241 | None:intergenic | 30.0% | |
| ACTAATTCCATTTCCAAAAG+GGG | + | chr1.3:48582774-48582793 | None:intergenic | 30.0% | |
| ATACTCAACTTCATTTCCAT+TGG | + | chr1.3:48581608-48581627 | None:intergenic | 30.0% | |
| CATGCTTGTGATTATAAATC+AGG | - | chr1.3:48581721-48581740 | MS.gene24021:intron | 30.0% | |
| GATATTCAGGAAATTCTTGT+GGG | + | chr1.3:48581453-48581472 | None:intergenic | 30.0% | |
| GCTAAATATGATGAGACTAA+TGG | - | chr1.3:48581559-48581578 | MS.gene24021:intron | 30.0% | |
| GGAATTAAGAGGTTTACTAA+TGG | - | chr1.3:48582404-48582423 | MS.gene24021:intron | 30.0% | |
| GTAGTTGACAGAATTGTTTA+GGG | + | chr1.3:48581417-48581436 | None:intergenic | 30.0% | |
| TGCAAATTTGATGTTTGGTT+CGG | - | chr1.3:48582621-48582640 | MS.gene24021:CDS | 30.0% | |
| TGTGATTATAAATCAGGTGA+AGG | - | chr1.3:48581727-48581746 | MS.gene24021:intron | 30.0% | |
| TGTTTCTTTGTTGGATATTC+AGG | + | chr1.3:48581466-48581485 | None:intergenic | 30.0% | |
| TTAGTAAACCTCTTAATTCC+AGG | + | chr1.3:48582404-48582423 | None:intergenic | 30.0% | |
| TTCTATTGTTCTTCGTAGTT+CGG | - | chr1.3:48581837-48581856 | MS.gene24021:CDS | 30.0% | |
| ! | TTTTGCCTAGAGAAGTTTTT+GGG | - | chr1.3:48582153-48582172 | MS.gene24021:intron | 30.0% |
| ! | TTTTTACCTTTTTCCCCTTT+TGG | - | chr1.3:48582758-48582777 | MS.gene24021:CDS | 30.0% |
| !! | GTTTTTGGGAAATCAACATT+TGG | - | chr1.3:48582167-48582186 | MS.gene24021:intron | 30.0% |
| !! | TTTGGTTCATTTTTGTGTGT+AGG | - | chr1.3:48582833-48582852 | MS.gene24021:CDS | 30.0% |
| !! | TTTTAGAGGGAAGAAAGTTT+TGG | - | chr1.3:48581750-48581769 | MS.gene24021:intron | 30.0% |
| !!! | AGATTCTTTTGATTTTGGCT+TGG | - | chr1.3:48582237-48582256 | MS.gene24021:intron | 30.0% |
| !!! | GGTCGAAGATTTTTAAGTTT+GGG | - | chr1.3:48582005-48582024 | MS.gene24021:CDS | 30.0% |
| !!! | GTCGAAGATTTTTAAGTTTG+GGG | - | chr1.3:48582006-48582025 | MS.gene24021:CDS | 30.0% |
| !!! | TGGTCGAAGATTTTTAAGTT+TGG | - | chr1.3:48582004-48582023 | MS.gene24021:CDS | 30.0% |
| ACAGTTGAACCATGATTCAT+AGG | - | chr1.3:48582595-48582614 | MS.gene24021:CDS | 35.0% | |
| ACCTTGGAGAAAATTAGATC+TGG | - | chr1.3:48582362-48582381 | MS.gene24021:intron | 35.0% | |
| ATCTTGTCAACTATCCAAAC+GGG | + | chr1.3:48582221-48582240 | None:intergenic | 35.0% | |
| CTCAAGATATTGCTCAAGTT+TGG | - | chr1.3:48582991-48583010 | MS.gene24021:CDS | 35.0% | |
| GAACTACGAAGAACAATAGA+AGG | + | chr1.3:48581838-48581857 | None:intergenic | 35.0% | |
| GAAGGGTTGAGTAAATTCAA+AGG | - | chr1.3:48581688-48581707 | MS.gene24021:intron | 35.0% | |
| GATTTCCCAAAAACTTCTCT+AGG | + | chr1.3:48582161-48582180 | None:intergenic | 35.0% | |
| GGATATTCAGGAAATTCTTG+TGG | + | chr1.3:48581454-48581473 | None:intergenic | 35.0% | |
| GGTAGTTGACAGAATTGTTT+AGG | + | chr1.3:48581418-48581437 | None:intergenic | 35.0% | |
| GGTCTTGCAAATTTGATGTT+TGG | - | chr1.3:48582616-48582635 | MS.gene24021:CDS | 35.0% | |
| GTAAACGGTCCTATAATAGT+AGG | - | chr1.3:48581259-48581278 | MS.gene24021:CDS | 35.0% | |
| TGGGAAATCAACATTTGGAT+TGG | - | chr1.3:48582172-48582191 | MS.gene24021:intron | 35.0% | |
| TTACTCAACCCTTCAATATC+AGG | + | chr1.3:48581682-48581701 | None:intergenic | 35.0% | |
| TTGGAGCTTAAGAACACAAA+GGG | - | chr1.3:48582314-48582333 | MS.gene24021:intron | 35.0% | |
| TTTGCAAGACCTATGAATCA+TGG | + | chr1.3:48582607-48582626 | None:intergenic | 35.0% | |
| TTTGTGTTCTTAAGCTCCAA+AGG | + | chr1.3:48582314-48582333 | None:intergenic | 35.0% | |
| ! | ACCAGATCTAATTTTCTCCA+AGG | + | chr1.3:48582366-48582385 | None:intergenic | 35.0% |
| ! | GGTGAATTTTTCTCCAAGAA+TGG | - | chr1.3:48582858-48582877 | MS.gene24021:CDS | 35.0% |
| ! | GTTTTGCCTAGAGAAGTTTT+TGG | - | chr1.3:48582152-48582171 | MS.gene24021:intron | 35.0% |
| ! | TGACTTTGGAAATCCATTCT+TGG | + | chr1.3:48582874-48582893 | None:intergenic | 35.0% |
| ! | TGGCAGTTATGATGATGAAA+TGG | - | chr1.3:48582192-48582211 | MS.gene24021:intron | 35.0% |
| ! | TTTCTTCTTCGTTATCGACT+AGG | + | chr1.3:48581216-48581235 | None:intergenic | 35.0% |
| ! | TTTGGAGCTTAAGAACACAA+AGG | - | chr1.3:48582313-48582332 | MS.gene24021:intron | 35.0% |
| !! | AAATCAGGTGAAGGTTTTAG+AGG | - | chr1.3:48581736-48581755 | MS.gene24021:intron | 35.0% |
| !! | GTTCATTTTTGTGTGTAGGA+AGG | - | chr1.3:48582837-48582856 | MS.gene24021:CDS | 35.0% |
| !! | TTCAACTGTTTTGAACTGCT+CGG | + | chr1.3:48582584-48582603 | None:intergenic | 35.0% |
| !! | TTGCTCTGAAAGAACTAATG+TGG | - | chr1.3:48581984-48582003 | MS.gene24021:CDS | 35.0% |
| !!! | AAAGTTTTGGTTGTTGGTTG+TGG | - | chr1.3:48581763-48581782 | MS.gene24021:CDS | 35.0% |
| !!! | AATCAGGTGAAGGTTTTAGA+GGG | - | chr1.3:48581737-48581756 | MS.gene24021:intron | 35.0% |
| !!! | ATTTTGGCTTGGTTAGTGTT+TGG | - | chr1.3:48582248-48582267 | MS.gene24021:intron | 35.0% |
| !!! | TGTTGTGTTTTTGGTTGAAC+AGG | - | chr1.3:48582124-48582143 | MS.gene24021:intron | 35.0% |
| AAGTTGTGCCTGGAATTAAG+AGG | - | chr1.3:48582393-48582412 | MS.gene24021:intron | 40.0% | |
| AATGGTTGGAAAGGAACTGT+TGG | - | chr1.3:48582903-48582922 | MS.gene24021:CDS | 40.0% | |
| ACAGTTCCTTTCCAACCATT+AGG | + | chr1.3:48582903-48582922 | None:intergenic | 40.0% | |
| ACTGTTGGACTTTATGCTGT+TGG | - | chr1.3:48582918-48582937 | MS.gene24021:CDS | 40.0% | |
| AGAGTTGAGACAGTTTCCAA+TGG | - | chr1.3:48581589-48581608 | MS.gene24021:intron | 40.0% | |
| AGGTGCCAATATCCAAAACA+GGG | + | chr1.3:48582346-48582365 | None:intergenic | 40.0% | |
| AGTCTCACATCGTTGCATTT+GGG | - | chr1.3:48581237-48581256 | MS.gene24021:CDS | 40.0% | |
| CAAATTGCATAGCTTCACTG+TGG | - | chr1.3:48581356-48581375 | MS.gene24021:CDS | 40.0% | |
| CAGTTCCTTTCCAACCATTA+GGG | + | chr1.3:48582902-48582921 | None:intergenic | 40.0% | |
| CATCGTTGCATTTGGGTAAA+CGG | - | chr1.3:48581244-48581263 | MS.gene24021:CDS | 40.0% | |
| CCATTTCCAAAAGGGGAAAA+AGG | + | chr1.3:48582767-48582786 | None:intergenic | 40.0% | |
| CTGTTGGACTTTATGCTGTT+GGG | - | chr1.3:48582919-48582938 | MS.gene24021:CDS | 40.0% | |
| CTGTTGGGTTTACAAAGAGA+GGG | - | chr1.3:48582934-48582953 | MS.gene24021:CDS | 40.0% | |
| GATCTTTCAAGCACCATGAA+GGG | + | chr1.3:48581337-48581356 | None:intergenic | 40.0% | |
| GCAGCATGTCTTAAACAACA+AGG | - | chr1.3:48581307-48581326 | MS.gene24021:CDS | 40.0% | |
| GTATATTTGTAGGTCGCTTG+TGG | - | chr1.3:48581624-48581643 | MS.gene24021:intron | 40.0% | |
| GTGTTGTGCCTGATATTGAA+GGG | - | chr1.3:48581671-48581690 | MS.gene24021:intron | 40.0% | |
| GTTGACAGAATTGTTTAGGG+AGG | + | chr1.3:48581414-48581433 | None:intergenic | 40.0% | |
| TAGTCTCACATCGTTGCATT+TGG | - | chr1.3:48581236-48581255 | MS.gene24021:CDS | 40.0% | |
| TCAGGAAATTCTTGTGGGAA+TGG | + | chr1.3:48581448-48581467 | None:intergenic | 40.0% | |
| TCTTGTGGGAATGGAAAGTT+AGG | + | chr1.3:48581439-48581458 | None:intergenic | 40.0% | |
| TGATCTTTCAAGCACCATGA+AGG | + | chr1.3:48581338-48581357 | None:intergenic | 40.0% | |
| TGATGAAATGGTTACCCGTT+TGG | - | chr1.3:48582204-48582223 | MS.gene24021:intron | 40.0% | |
| TGTGTTGTGCCTGATATTGA+AGG | - | chr1.3:48581670-48581689 | MS.gene24021:intron | 40.0% | |
| TTCTCACCTTAAGCCAAGAA+GGG | + | chr1.3:48582505-48582524 | None:intergenic | 40.0% | |
| TTTCTCACCTTAAGCCAAGA+AGG | + | chr1.3:48582506-48582525 | None:intergenic | 40.0% | |
| ! | AAGGTGCCAATATCCAAAAC+AGG | + | chr1.3:48582347-48582366 | None:intergenic | 40.0% |
| ! | GTATGGTCTTAAAAGGCCAT+TGG | - | chr1.3:48582283-48582302 | MS.gene24021:intron | 40.0% |
| ! | GTTGTTGGTTGTGGTAATTC+TGG | - | chr1.3:48581772-48581791 | MS.gene24021:CDS | 40.0% |
| ! | TGGTTGTGGTAATTCTGGAA+TGG | - | chr1.3:48581777-48581796 | MS.gene24021:CDS | 40.0% |
| !! | AAGACCCCTGTTTTGGATAT+TGG | - | chr1.3:48582338-48582357 | MS.gene24021:intron | 40.0% |
| !! | ATGATGAGACTAATGGGTTG+TGG | - | chr1.3:48581566-48581585 | MS.gene24021:intron | 40.0% |
| !! | CCTTTTTCCCCTTTTGGAAA+TGG | - | chr1.3:48582764-48582783 | MS.gene24021:CDS | 40.0% |
| !! | GGCTGTGTTGAACTTGTTAA+TGG | - | chr1.3:48582425-48582444 | MS.gene24021:CDS | 40.0% |
| !! | TGTTTTGGATATTGGCACCT+TGG | - | chr1.3:48582346-48582365 | MS.gene24021:intron | 40.0% |
| !!! | GGGAAGAAAGTTTTGGTTGT+TGG | - | chr1.3:48581757-48581776 | MS.gene24021:intron | 40.0% |
| !!! | GTTCGGTTTTGAAACATCGT+TGG | - | chr1.3:48582638-48582657 | MS.gene24021:CDS | 40.0% |
| AGTCACCATTCCCTAATGGT+TGG | - | chr1.3:48582889-48582908 | MS.gene24021:CDS | 45.0% | |
| ATCGTAGCAATGTCCCTTCT+TGG | - | chr1.3:48582489-48582508 | MS.gene24021:CDS | 45.0% | |
| CAATGTCCCTTCTTGGCTTA+AGG | - | chr1.3:48582496-48582515 | MS.gene24021:CDS | 45.0% | |
| CCAAAGTCACCATTCCCTAA+TGG | - | chr1.3:48582885-48582904 | MS.gene24021:CDS | 45.0% | |
| CCATTAGGGAATGGTGACTT+TGG | + | chr1.3:48582888-48582907 | None:intergenic | 45.0% | |
| CCATTCCCTAATGGTTGGAA+AGG | - | chr1.3:48582894-48582913 | MS.gene24021:CDS | 45.0% | |
| CCTTTCCAACCATTAGGGAA+TGG | + | chr1.3:48582897-48582916 | None:intergenic | 45.0% | |
| GAACACACTCGTTGAATCGT+GGG | + | chr1.3:48581534-48581553 | None:intergenic | 45.0% | |
| GATGCAGTTGTTCTTGCTAC+TGG | - | chr1.3:48582464-48582483 | MS.gene24021:CDS | 45.0% | |
| GCTGTTGGGTTTACAAAGAG+AGG | - | chr1.3:48582933-48582952 | MS.gene24021:CDS | 45.0% | |
| GGTCACATAAAAGTTGTGCC+TGG | - | chr1.3:48582383-48582402 | MS.gene24021:intron | 45.0% | |
| GGTCCTATAATAGTAGGAGC+AGG | - | chr1.3:48581265-48581284 | MS.gene24021:CDS | 45.0% | |
| GGTCCTGCTCCTACTATTAT+AGG | + | chr1.3:48581271-48581290 | None:intergenic | 45.0% | |
| GGTCTTAAAAGGCCATTGGA+GGG | - | chr1.3:48582287-48582306 | MS.gene24021:intron | 45.0% | |
| GGTGCCAATATCCAAAACAG+GGG | + | chr1.3:48582345-48582364 | None:intergenic | 45.0% | |
| TGAACACACTCGTTGAATCG+TGG | + | chr1.3:48581535-48581554 | None:intergenic | 45.0% | |
| TTTACAAAGAGAGGGCTCTC+TGG | - | chr1.3:48582942-48582961 | MS.gene24021:CDS | 45.0% | |
| ! | AAAGGGTAAGACCCCTGTTT+TGG | - | chr1.3:48582331-48582350 | MS.gene24021:intron | 45.0% |
| ! | GATTTCGAAGTGTTTCGCGT+AGG | + | chr1.3:48581509-48581528 | None:intergenic | 45.0% |
| ! | TGGTCTTAAAAGGCCATTGG+AGG | - | chr1.3:48582286-48582305 | MS.gene24021:intron | 45.0% |
| !! | ACAACAAGGAGTACCCTTCA+TGG | - | chr1.3:48581321-48581340 | MS.gene24021:CDS | 45.0% |
| !!! | AAAAAAATTATTTTATGTAT+TGG | + | chr1.3:48582711-48582730 | None:intergenic | 5.0% |
| !!! | TTTTTAACAAAAATAATTTA+AGG | - | chr1.3:48582066-48582085 | MS.gene24021:CDS | 5.0% |
| ATAGTAGGAGCAGGACCATC+AGG | - | chr1.3:48581274-48581293 | MS.gene24021:CDS | 50.0% | |
| AAGGCCATTGGAGGGTCCTT+TGG | - | chr1.3:48582295-48582314 | MS.gene24021:intron | 55.0% | |
| AGCTCCAAAGGACCCTCCAA+TGG | + | chr1.3:48582302-48582321 | None:intergenic | 55.0% | |
| AGGTCGCTTGTGGTTGCTAC+TGG | - | chr1.3:48581634-48581653 | MS.gene24021:intron | 55.0% | |
| GCTGCTGTTGCTAGACCTGA+TGG | + | chr1.3:48581292-48581311 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 48581196 | 48583082 | 48581196 | ID=MS.gene24021 |
| chr1.3 | mRNA | 48581196 | 48583082 | 48581196 | ID=MS.gene24021.t1;Parent=MS.gene24021 |
| chr1.3 | exon | 48582420 | 48583082 | 48582420 | ID=MS.gene24021.t1.exon1;Parent=MS.gene24021.t1 |
| chr1.3 | CDS | 48582420 | 48583082 | 48582420 | ID=cds.MS.gene24021.t1;Parent=MS.gene24021.t1 |
| chr1.3 | exon | 48581761 | 48582132 | 48581761 | ID=MS.gene24021.t1.exon2;Parent=MS.gene24021.t1 |
| chr1.3 | CDS | 48581761 | 48582132 | 48581761 | ID=cds.MS.gene24021.t1;Parent=MS.gene24021.t1 |
| chr1.3 | exon | 48581196 | 48581423 | 48581196 | ID=MS.gene24021.t1.exon3;Parent=MS.gene24021.t1 |
| chr1.3 | CDS | 48581196 | 48581423 | 48581196 | ID=cds.MS.gene24021.t1;Parent=MS.gene24021.t1 |
| Gene Sequence |
| Protein sequence |
