AlfalfaGEDB | MS.gene24050

M. sativa cultivar XinJiangDaYe / MS.gene24050

KEGG: K12492
GO: GO:0003674 GO:0005096 GO:0008047 GO:0030234 GO:0030695 GO:0060589 GO:0098772
PFAM: PF01412.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24050.t1	XP_013468351.1	97.7	481	11	0	1	481	1	481	5.10E-224	786.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24050.t1	O80925	69.0	368	102	6	1	359	1	365	6.7e-132	472.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24050.t1	A0A072VLX3	97.7	481	11	0	1	481	1	481	3.7e-224	786.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049411	MS.gene24050	0.839551	1.48E-57	-1.69E-46
MS.gene049680	MS.gene24050	0.802144	6.45E-49	-1.69E-46
MS.gene049807	MS.gene24050	0.812214	4.75E-51	-1.69E-46
MS.gene050646	MS.gene24050	0.821838	3.26E-53	-1.69E-46
MS.gene050945	MS.gene24050	0.827428	1.57E-54	-1.69E-46
MS.gene051234	MS.gene24050	0.810075	1.38E-50	-1.69E-46
MS.gene051699	MS.gene24050	0.805555	1.26E-49	-1.69E-46
MS.gene051731	MS.gene24050	0.810046	1.40E-50	-1.69E-46
MS.gene052072	MS.gene24050	0.84347	1.37E-58	-1.69E-46
MS.gene052628	MS.gene24050	0.807649	4.56E-50	-1.69E-46
MS.gene052630	MS.gene24050	0.804944	1.69E-49	-1.69E-46
MS.gene052793	MS.gene24050	0.853642	2.09E-61	-1.69E-46
MS.gene053143	MS.gene24050	0.811954	5.41E-51	-1.69E-46
MS.gene053427	MS.gene24050	0.835929	1.26E-56	-1.69E-46
MS.gene054004	MS.gene24050	0.835405	1.71E-56	-1.69E-46
MS.gene054142	MS.gene24050	0.803526	3.34E-49	-1.69E-46
MS.gene054709	MS.gene24050	0.831774	1.38E-55	-1.69E-46
MS.gene055339	MS.gene24050	0.900945	4.41E-78	-1.69E-46
MS.gene055506	MS.gene24050	0.805513	1.29E-49	-1.69E-46
MS.gene055765	MS.gene24050	0.82105	4.96E-53	-1.69E-46
MS.gene056082	MS.gene24050	0.81191	5.53E-51	-1.69E-46
MS.gene056578	MS.gene24050	0.81565	8.29E-52	-1.69E-46
MS.gene056677	MS.gene24050	0.805983	1.03E-49	-1.69E-46
MS.gene056881	MS.gene24050	0.835157	1.98E-56	-1.69E-46
MS.gene057102	MS.gene24050	0.807777	4.28E-50	-1.69E-46
MS.gene057694	MS.gene24050	0.829361	5.38E-55	-1.69E-46
MS.gene058781	MS.gene24050	0.803153	3.99E-49	-1.69E-46
MS.gene059778	MS.gene24050	0.84573	3.38E-59	-1.69E-46
MS.gene059802	MS.gene24050	0.852652	4.01E-61	-1.69E-46
MS.gene059949	MS.gene24050	0.810464	1.14E-50	-1.69E-46
MS.gene060937	MS.gene24050	0.804098	2.54E-49	-1.69E-46
MS.gene061100	MS.gene24050	0.845467	3.99E-59	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene67376	MS.gene24050	PPI
MS.gene24050	MS.gene052122	PPI
MS.gene24050	MS.gene021049	PPI
MS.gene45489	MS.gene24050	PPI
MS.gene79962	MS.gene24050	PPI
MS.gene30955	MS.gene24050	PPI
MS.gene021114	MS.gene24050	PPI
MS.gene24050	MS.gene071169	PPI
MS.gene044849	MS.gene24050	PPI
MS.gene004045	MS.gene24050	PPI
MS.gene24050	MS.gene036453	PPI
MS.gene004101	MS.gene24050	PPI
MS.gene31156	MS.gene24050	PPI
MS.gene24050	MS.gene41176	PPI
MS.gene001874	MS.gene24050	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24050.t1	MTR_1g069000	98.545	481	7	0	1	481	1	481	0.0	977
MS.gene24050.t1	MTR_7g099830	64.989	457	102	10	1	444	1	412	0.0	557
MS.gene24050.t1	MTR_8g067090	48.039	102	53	0	7	108	13	114	1.33e-27	114
MS.gene24050.t1	MTR_8g471120	48.039	102	53	0	7	108	13	114	1.33e-27	114
MS.gene24050.t1	MTR_8g067080	44.762	105	58	0	7	111	13	117	2.85e-27	113
MS.gene24050.t1	MTR_8g067080	44.762	105	58	0	7	111	13	117	3.48e-27	113
MS.gene24050.t1	MTR_8g471110	44.762	105	58	0	7	111	13	117	3.48e-27	113
MS.gene24050.t1	MTR_2g030230	48.864	88	43	2	5	91	19	105	4.44e-21	94.4
MS.gene24050.t1	MTR_8g088320	46.739	92	47	2	1	91	6	96	6.92e-21	93.6
MS.gene24050.t1	MTR_2g080950	48.101	79	41	0	5	83	17	95	2.78e-20	94.0
MS.gene24050.t1	MTR_8g464390	48.101	79	41	0	5	83	17	95	9.69e-20	92.0
MS.gene24050.t1	MTR_6g080710	51.351	74	36	0	4	77	16	89	5.96e-19	87.0
MS.gene24050.t1	MTR_8g038400	48.718	78	39	1	5	81	18	95	1.60e-18	87.4
MS.gene24050.t1	MTR_3g017500	48.052	77	39	1	6	81	23	99	1.82e-17	84.3
MS.gene24050.t1	MTR_3g017500	48.052	77	39	1	6	81	53	129	3.09e-17	84.0
MS.gene24050.t1	MTR_4g074170	46.053	76	39	1	10	83	485	560	4.16e-15	78.6
MS.gene24050.t1	MTR_8g100100	48.571	70	34	1	10	77	500	569	2.11e-14	76.3
MS.gene24050.t1	MTR_8g100100	48.571	70	34	1	10	77	506	575	2.16e-14	76.3
MS.gene24050.t1	MTR_7g020860	45.333	75	39	1	10	82	508	582	1.05e-13	73.9
MS.gene24050.t1	MTR_7g020860	47.143	70	35	1	10	77	508	577	1.24e-13	73.9
MS.gene24050.t1	MTR_3g098680	44.286	70	37	1	10	77	503	572	5.40e-12	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24050.t1	AT2G37550	61.789	492	141	8	1	481	1	456	0.0	554
MS.gene24050.t1	AT2G37550	61.789	492	141	8	1	481	1	456	0.0	554
MS.gene24050.t1	AT3G53710	61.647	498	135	13	1	481	1	459	0.0	541
MS.gene24050.t1	AT3G53710	61.647	498	135	13	1	481	1	459	0.0	541
MS.gene24050.t1	AT3G53710	61.647	498	135	13	1	481	1	459	0.0	541
MS.gene24050.t1	AT4G17890	48.571	105	54	0	7	111	16	120	1.37e-29	119
MS.gene24050.t1	AT4G17890	48.571	105	54	0	7	111	16	120	2.18e-29	119
MS.gene24050.t1	AT5G46750	48.571	105	54	0	7	111	13	117	6.64e-29	118
MS.gene24050.t1	AT2G35210	46.667	105	56	0	7	111	13	117	1.05e-27	114
MS.gene24050.t1	AT2G35210	46.667	105	56	0	7	111	13	117	2.83e-27	113
MS.gene24050.t1	AT4G05330	48.810	84	42	1	5	87	16	99	4.08e-22	97.8
MS.gene24050.t1	AT4G05330	48.810	84	42	1	5	87	16	99	4.08e-22	97.8
MS.gene24050.t1	AT5G54310	41.803	122	68	2	7	128	19	137	1.24e-21	97.8
MS.gene24050.t1	AT3G17660	52.703	74	35	0	4	77	16	89	2.51e-21	91.7
MS.gene24050.t1	AT3G17660	52.703	74	35	0	4	77	16	89	3.19e-21	92.8
MS.gene24050.t1	AT4G21160	47.727	88	44	2	5	91	16	102	4.46e-21	94.7
MS.gene24050.t1	AT4G21160	47.727	88	44	2	5	91	16	102	4.46e-21	94.7
MS.gene24050.t1	AT4G21160	47.727	88	44	2	5	91	16	102	4.46e-21	94.7
MS.gene24050.t1	AT4G21160	47.727	88	44	2	5	91	16	102	4.46e-21	94.7
MS.gene24050.t1	AT3G17660	52.703	74	35	0	4	77	16	89	8.84e-21	92.0
MS.gene24050.t1	AT3G07940	50.649	77	37	1	6	81	49	125	9.03e-20	89.7
MS.gene24050.t1	AT3G07940	50.649	77	37	1	6	81	49	125	3.23e-19	89.7
MS.gene24050.t1	AT1G60860	54.386	57	26	0	7	63	359	415	2.53e-15	79.0
MS.gene24050.t1	AT1G60860	54.386	57	26	0	7	63	470	526	2.55e-15	79.3
MS.gene24050.t1	AT1G60860	54.386	57	26	0	7	63	299	355	2.91e-15	79.0
MS.gene24050.t1	AT3G17660	50.000	58	29	0	4	61	16	73	3.63e-14	72.0
MS.gene24050.t1	AT5G13300	48.571	70	34	1	10	77	507	576	3.83e-14	75.5
MS.gene24050.t1	AT5G61980	45.714	70	36	1	15	82	509	578	1.09e-13	74.3
MS.gene24050.t1	AT1G10870	50.000	54	27	0	10	63	473	526	3.17e-13	72.8
MS.gene24050.t1	AT1G10870	50.000	54	27	0	10	63	473	526	3.38e-13	72.4

Find 169 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGGATAGGGAAGGAGGTTC+GGG	0.309762	1.3:+48224458	MS.gene24050:CDS
AAGAAAGCTGCTGGACATAA+TGG	0.311171	1.3:+48226457	MS.gene24050:CDS
CACAAAAGAGATTACTTCTA+AGG	0.329121	1.3:+48225026	MS.gene24050:CDS
CTCAAACTCTTGGGACGATT+GGG	0.331416	1.3:+48226201	MS.gene24050:CDS
TGGCGGCATCACGGCGGCTC+CGG	0.342322	1.3:+48224006	MS.gene24050:CDS
TTGGGATAGGGAAGGAGGTT+CGG	0.345457	1.3:+48224457	MS.gene24050:CDS
ATCCCAGCTCTTGGGATGAT+TGG	0.345579	1.3:+48226305	MS.gene24050:CDS
ATGATGCTGGTTGGGCTGGC+TGG	0.346920	1.3:+48226383	MS.gene24050:CDS
TATTTATTCGCGGTCGCAAT+TGG	0.352308	1.3:+48224565	MS.gene24050:CDS
GCAGATCTTGTTGCCGGGTT+GGG	0.371767	1.3:-48224039	None:intergenic
CAAATTCGACTACTAATAAC+TGG	0.372531	1.3:+48226062	MS.gene24050:CDS
CCGGTTGTGAAGGAGAATTT+AGG	0.374910	1.3:+48224374	MS.gene24050:CDS
GGAGTTGATCCTTTATTAGT+AGG	0.375169	1.3:-48226250	None:intergenic
ATGATTCCGGCTGGGATGAT+TGG	0.384077	1.3:+48224438	MS.gene24050:CDS
TATTCATCTGGTGGGCAGTC+TGG	0.394756	1.3:+48226274	MS.gene24050:CDS
AGTTTATCGTGATCGGATTC+AGG	0.397292	1.3:+48224319	MS.gene24050:CDS
CTTCCTTCCTAGAATCTTTG+TGG	0.398078	1.3:-48226330	None:intergenic
AAGGGAGAACCTACTAATAA+AGG	0.401153	1.3:+48226241	MS.gene24050:CDS
GACTGCCCACCAGATGAATA+AGG	0.405430	1.3:-48226271	None:intergenic
TGATAGAAATGGCTACTATC+AGG	0.410799	1.3:+48226099	MS.gene24050:CDS
CGCAGATCTTGTTGCCGGGT+TGG	0.414757	1.3:-48224040	None:intergenic
TGTTGCCGGGTTGGGATTGA+AGG	0.414970	1.3:-48224031	None:intergenic
GCTCAAACTCTTGGGACGAT+TGG	0.416102	1.3:+48226200	MS.gene24050:CDS
ATTGGGACCACAAAGATTCT+AGG	0.416709	1.3:+48226323	MS.gene24050:CDS
CCACAATAATGATGCTGGTT+GGG	0.420450	1.3:+48226375	MS.gene24050:CDS
TCCCAGCTCTTGGGATGATT+GGG	0.422693	1.3:+48226306	MS.gene24050:CDS
GGCGGCATCACGGCGGCTCC+GGG	0.423745	1.3:+48224007	MS.gene24050:CDS
CGCCGATGGGGCAGAGTCGC+CGG	0.424686	1.3:+48224411	MS.gene24050:CDS
CGAAATGAGAATGATAGAAA+TGG	0.430727	1.3:+48226088	MS.gene24050:CDS
AAACTCAAAGCTCAAACTCT+TGG	0.431090	1.3:+48226191	MS.gene24050:CDS
AATAATGATGCTGGTTGGGC+TGG	0.431540	1.3:+48226379	MS.gene24050:CDS
TCGGTCGTGGCGTGATCCTC+CGG	0.431712	1.3:+48224355	MS.gene24050:CDS
TTCCTTCGGAATAGAATACT+TGG	0.434318	1.3:-48224258	None:intergenic
TGATTCCGGCTGGGATGATT+GGG	0.435890	1.3:+48224439	MS.gene24050:CDS
TACTATCAGGATTTCAATCA+AGG	0.437121	1.3:+48226112	MS.gene24050:CDS
CGCAAGGTGGGAAGTATGTT+GGG	0.437210	1.3:+48224669	MS.gene24050:CDS
TACTCACAATCCCAGCTCTT+GGG	0.438114	1.3:+48226297	MS.gene24050:CDS
GGATCAACTCCTTATTCATC+TGG	0.439382	1.3:+48226262	MS.gene24050:CDS
GGAAGAGAACAATCTTCATC+AGG	0.440220	1.3:+48226160	MS.gene24050:CDS
AAATCAGATGACGCTTGGAC+AGG	0.441545	1.3:+48226481	MS.gene24050:CDS
GGGGATTGGTAAGTTGTCAT+TGG	0.442974	1.3:+48224969	MS.gene24050:intron
AGAAAGCTGCTGGACATAAT+GGG	0.444118	1.3:+48226458	MS.gene24050:CDS
CCTAAATTCTCCTTCACAAC+CGG	0.446662	1.3:-48224374	None:intergenic
TCCGGAAAACACCGTGGTCT+CGG	0.447372	1.3:+48224131	MS.gene24050:CDS
AACTCAAAGCTCAAACTCTT+GGG	0.448408	1.3:+48226192	MS.gene24050:CDS
TGGGGAGCTGATGCTTTAGC+TGG	0.452701	1.3:-48226355	None:intergenic
CATGGCTTTGGCTTCACAGA+AGG	0.454790	1.3:+48226015	MS.gene24050:CDS
TGCGACGGTGGAAGTCCTTC+CGG	0.458253	1.3:-48224650	None:intergenic
AAGGAGAATTTAGGTTCAAA+GGG	0.460070	1.3:+48224383	MS.gene24050:CDS
ATCGACGCAGATCTTGTTGC+CGG	0.461374	1.3:-48224045	None:intergenic
AAAGGAGGTAATTCTTCTAC+TGG	0.461732	1.3:+48226139	MS.gene24050:CDS
CGGATTCAGGCTTTGTCGGA+AGG	0.463866	1.3:+48224332	MS.gene24050:CDS
GTCCCAATCATCCCAAGAGC+TGG	0.464845	1.3:-48226308	None:intergenic
TGATCGGATTCAGGCTTTGT+CGG	0.466457	1.3:+48224328	MS.gene24050:CDS
GTAACAATATCCGTTTCCTT+CGG	0.466590	1.3:-48224272	None:intergenic
TTCAAAGGGAAGGCCGCCGA+TGG	0.468886	1.3:+48224397	MS.gene24050:CDS
AATCAGATGACGCTTGGACA+GGG	0.470359	1.3:+48226482	MS.gene24050:CDS
TGATGCTGGTTGGGCTGGCT+GGG	0.473809	1.3:+48226384	MS.gene24050:CDS
GTACTCACAATCCCAGCTCT+TGG	0.474135	1.3:+48226296	MS.gene24050:CDS
CTGCTTCAGTTTATCGTGAT+CGG	0.474442	1.3:+48224312	MS.gene24050:CDS
GAAGGAGAATTTAGGTTCAA+AGG	0.482185	1.3:+48224382	MS.gene24050:CDS
AGCTTCTGCGGCGGGTAAAG+AGG	0.482654	1.3:+48224589	MS.gene24050:CDS
CAGAGATTGGTCAAAGGACA+TGG	0.483037	1.3:+48225975	MS.gene24050:CDS
CCCAACCAGCATCATTATTG+TGG	0.485486	1.3:-48226375	None:intergenic
TTTGAACGGCGGTGGTGCAC+CGG	0.486910	1.3:+48224520	MS.gene24050:CDS
AGCAGCGTTGGTGTTATACT+TGG	0.487190	1.3:-48224294	None:intergenic
ATTTAATTATCACAGGGGAT+TGG	0.494169	1.3:+48224955	MS.gene24050:intron
AGTCGCCGGAGTAATGATTC+CGG	0.494324	1.3:+48224425	MS.gene24050:CDS
AAGGACATGGGGTTTAATGA+AGG	0.494662	1.3:+48225988	MS.gene24050:CDS
TCATCTAATAAGAAAGCTGC+TGG	0.498028	1.3:+48226448	MS.gene24050:CDS
TCAAAGGGAAGGCCGCCGAT+GGG	0.498527	1.3:+48224398	MS.gene24050:CDS
GATAGGGAAGGAGGTTCGGG+TGG	0.503166	1.3:+48224461	MS.gene24050:CDS
GCCGGAGTAATGATTCCGGC+TGG	0.503400	1.3:+48224429	MS.gene24050:CDS
GGTGGGAAGTATGTTGGGTT+CGG	0.503415	1.3:+48224674	MS.gene24050:CDS
GCTGCTAATGCTGTTCAGAC+TGG	0.505364	1.3:+48225003	MS.gene24050:CDS
GGAAGGCGGTTATGATCAAA+AGG	0.509923	1.3:+48225916	MS.gene24050:CDS
CGGGTTGGGATTGAAGGTCC+CGG	0.513419	1.3:-48224025	None:intergenic
TCGCAAGGTGGGAAGTATGT+TGG	0.513815	1.3:+48224668	MS.gene24050:CDS
CCCACAATAATGATGCTGGT+TGG	0.515926	1.3:+48226374	MS.gene24050:CDS
GACTTCCACCGTCGCAAGGT+GGG	0.517045	1.3:+48224657	MS.gene24050:CDS
TTTGGCTTCACAGAAGGTTG+AGG	0.518131	1.3:+48226021	MS.gene24050:CDS
CAATTGGAAGCTTCTGCGGC+GGG	0.519075	1.3:+48224581	MS.gene24050:CDS
CTTTGTCGGAAGGTCGGTCG+TGG	0.519720	1.3:+48224342	MS.gene24050:CDS
CCAACCAGCATCATTATTGT+GGG	0.519854	1.3:-48226374	None:intergenic
ACTCAAAAGACATCAGAGAT+TGG	0.520352	1.3:+48225962	MS.gene24050:CDS
TTGCTCCTTTGTGAAGGCAT+CGG	0.521360	1.3:-48224704	None:intergenic
ATGGGAAATCAGATGACGCT+TGG	0.522747	1.3:+48226476	MS.gene24050:CDS
TGTGGATTGCTCCTTTGTGA+AGG	0.525196	1.3:-48224710	None:intergenic
GATCCGTCACCATGGATGCT+TGG	0.527744	1.3:+48224174	MS.gene24050:CDS
GATTTCGGACCAAGCATCCA+TGG	0.529426	1.3:-48224183	None:intergenic
CCGGCTGGGATGATTGGGAT+AGG	0.534746	1.3:+48224444	MS.gene24050:CDS
TCGACGCAGATCTTGTTGCC+GGG	0.535740	1.3:-48224044	None:intergenic
ACGATAAACTGAAGCAGCGT+TGG	0.536105	1.3:-48224306	None:intergenic
TTCAGGCTTTGTCGGAAGGT+CGG	0.544881	1.3:+48224336	MS.gene24050:CDS
CTCCGGCGACTCTGCCCCAT+CGG	0.545912	1.3:-48224413	None:intergenic
TTTAGGAATTTGAACGGCGG+TGG	0.547735	1.3:+48224512	MS.gene24050:CDS
GCTCCCCACAATAATGATGC+TGG	0.549107	1.3:+48226370	MS.gene24050:CDS
GTATTCTATTCCGAAGGAAA+CGG	0.550054	1.3:+48224262	MS.gene24050:CDS
GCTTCTGCGGCGGGTAAAGA+GGG	0.555155	1.3:+48224590	MS.gene24050:CDS
GCGTGATCCTCCGGTTGTGA+AGG	0.563730	1.3:+48224364	MS.gene24050:CDS
TCCGTCGACCTCGATCTCGC+CGG	0.565057	1.3:-48224539	None:intergenic
ATCAGATGACGCTTGGACAG+GGG	0.565298	1.3:+48226483	MS.gene24050:CDS
TGCCAAGTATTCTATTCCGA+AGG	0.565535	1.3:+48224256	MS.gene24050:CDS
TCTTTGTTTCAGGTCAAGGA+AGG	0.567944	1.3:+48225899	MS.gene24050:intron
TACTTCCCACCTTGCGACGG+TGG	0.568939	1.3:-48224662	None:intergenic
GGCGGAGAATGAGTCAAGGC+CGG	0.569646	1.3:+48224631	MS.gene24050:CDS
GTCGCAATTGGAAGCTTCTG+CGG	0.569797	1.3:+48224577	MS.gene24050:CDS
CAAAGGGAAGGCCGCCGATG+GGG	0.571024	1.3:+48224399	MS.gene24050:CDS
GATCATCCATGGCGGCATCA+CGG	0.576667	1.3:+48223997	None:intergenic
AGAATTTAGGTTCAAAGGGA+AGG	0.577046	1.3:+48224387	MS.gene24050:CDS
TCAACTCCTTATTCATCTGG+TGG	0.577122	1.3:+48226265	MS.gene24050:CDS
CGGCTGGGATGATTGGGATA+GGG	0.577559	1.3:+48224445	MS.gene24050:CDS
TCGGAATAGAATACTTGGCA+AGG	0.577709	1.3:-48224253	None:intergenic
TCCCAATCATCCCAAGAGCT+GGG	0.579490	1.3:-48226307	None:intergenic
TTCATGTGCTTAGAATGCTC+CGG	0.579995	1.3:+48224113	MS.gene24050:CDS
GAAGGACTTCCACCGTCGCA+AGG	0.582983	1.3:+48224653	MS.gene24050:CDS
GAGCCGCCGTGATGCCGCCA+TGG	0.583473	1.3:-48224003	None:intergenic
TGGGATAATGCAAAAGATGA+TGG	0.583815	1.3:+48226403	MS.gene24050:CDS
CCTATCCCAATCATCCCAGC+CGG	0.584873	1.3:-48224444	None:intergenic
CCGGAGTAATGATTCCGGCT+GGG	0.585313	1.3:+48224430	MS.gene24050:CDS
TCAGATGACGCTTGGACAGG+GGG	0.587832	1.3:+48226484	MS.gene24050:CDS
GAGGTTCGGGTGGTGATGTG+AGG	0.591884	1.3:+48224471	MS.gene24050:CDS
GATGTGAGGAGTAAGTCGAC+GGG	0.593326	1.3:+48224485	MS.gene24050:CDS
TCAAACTCTTGGGACGATTG+GGG	0.596473	1.3:+48226202	MS.gene24050:CDS
AGGACATGGGGTTTAATGAA+GGG	0.599160	1.3:+48225989	MS.gene24050:CDS
GTGGTGCACCGGCGAGATCG+AGG	0.600418	1.3:+48224531	MS.gene24050:CDS
CCCAGCCGGAATCATTACTC+CGG	0.606336	1.3:-48224430	None:intergenic
CAGTGGGCATCAGTCTCCTA+CGG	0.607011	1.3:+48224086	MS.gene24050:CDS
CTCTCAGAAGAATCCACAGT+GGG	0.607546	1.3:+48224070	MS.gene24050:CDS
GCAATTGGAAGCTTCTGCGG+CGG	0.607693	1.3:+48224580	MS.gene24050:CDS
CGGGACCTTCAATCCCAACC+CGG	0.609873	1.3:+48224026	MS.gene24050:CDS
AAGCATCCATGGTGACGGAT+CGG	0.610332	1.3:-48224172	None:intergenic
TTGTTTCAGGTCAAGGAAGG+CGG	0.612416	1.3:+48225902	MS.gene24050:intron
TTCAATCAAGGGAACAAAGG+AGG	0.615582	1.3:+48226124	MS.gene24050:CDS
AGAGATTGGTCAAAGGACAT+GGG	0.615964	1.3:+48225976	MS.gene24050:CDS
GGACATGGGGTTTAATGAAG+GGG	0.616409	1.3:+48225990	MS.gene24050:CDS
GATTTCAATCAAGGGAACAA+AGG	0.616794	1.3:+48226121	MS.gene24050:CDS
GGACCAAGCATCCATGGTGA+CGG	0.618778	1.3:-48224177	None:intergenic
CATGGTGACGGATCGGACGA+AGG	0.619426	1.3:-48224165	None:intergenic
CGGCGACTCTGCCCCATCGG+CGG	0.622822	1.3:-48224410	None:intergenic
TGATGTGAGGAGTAAGTCGA+CGG	0.623072	1.3:+48224484	MS.gene24050:CDS
GAGAATGAGTCAAGGCCGGA+AGG	0.624456	1.3:+48224635	MS.gene24050:CDS
CTTCGTCCGATCCGTCACCA+TGG	0.629444	1.3:+48224166	MS.gene24050:CDS
GGACTTCCACCGTCGCAAGG+TGG	0.631829	1.3:+48224656	MS.gene24050:CDS
ACATACTTCCCACCTTGCGA+CGG	0.631937	1.3:-48224665	None:intergenic
GATGATTGGGATAGGGAAGG+AGG	0.632481	1.3:+48224452	MS.gene24050:CDS
AGACATCAGAGATTGGTCAA+AGG	0.632664	1.3:+48225969	MS.gene24050:CDS
CAACTCCTTATTCATCTGGT+GGG	0.634524	1.3:+48226266	MS.gene24050:CDS
GGACCACAAAGATTCTAGGA+AGG	0.638994	1.3:+48226327	MS.gene24050:CDS
CGACGGAGGATATTTATTCG+CGG	0.642927	1.3:+48224555	MS.gene24050:CDS
ACTATCAGGATTTCAATCAA+GGG	0.645448	1.3:+48226113	MS.gene24050:CDS
GAGATTGGTCAAAGGACATG+GGG	0.646826	1.3:+48225977	MS.gene24050:CDS
GAAGAGAACAATCTTCATCA+GGG	0.647329	1.3:+48226161	MS.gene24050:CDS
GGCGAGATCGAGGTCGACGG+AGG	0.649649	1.3:+48224541	MS.gene24050:CDS
CAACCAGCATCATTATTGTG+GGG	0.653135	1.3:-48226373	None:intergenic
ACCGGCGAGATCGAGGTCGA+CGG	0.653631	1.3:+48224538	MS.gene24050:CDS
TGGGATGATTGGGATAGGGA+AGG	0.657232	1.3:+48224449	MS.gene24050:CDS
AGATGTGAACACCGAGACCA+CGG	0.664997	1.3:-48224142	None:intergenic
TAAGCACATGAAGATGCCGT+AGG	0.669663	1.3:-48224102	None:intergenic
AAATTCTCCTTCACAACCGG+AGG	0.674563	1.3:-48224371	None:intergenic
AGATGGCGGAGAATGAGTCA+AGG	0.685000	1.3:+48224627	MS.gene24050:CDS
CTAGTCCGATGCCTTCACAA+AGG	0.693188	1.3:+48224699	MS.gene24050:CDS
GACATGGGGTTTAATGAAGG+GGG	0.697882	1.3:+48225991	MS.gene24050:CDS
ATGTGAGGAGTAAGTCGACG+GGG	0.706562	1.3:+48224486	MS.gene24050:CDS
TAGGAGACTGATGCCCACTG+TGG	0.710667	1.3:-48224083	None:intergenic
CATCCATGGCGGCATCACGG+CGG	0.720125	1.3:+48224000	None:intergenic
GAATGCTCCGGAAAACACCG+TGG	0.731228	1.3:+48224125	MS.gene24050:CDS
TGTGAGGAGTAAGTCGACGG+GGG	0.747661	1.3:+48224487	MS.gene24050:CDS
GCTCTCAGAAGAATCCACAG+TGG	0.805841	1.3:+48224069	MS.gene24050:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTGTATTTAATTATCACA+GGG	+	chr1.3:48224949-48224968	MS.gene24050:intron	15.0%
!!	TTATTGTATTTAATTATCAC+AGG	+	chr1.3:48224948-48224967	MS.gene24050:intron	15.0%
!!	AAAAAAGGAAAGAAACTAAT+TGG	-	chr1.3:48225738-48225757	None:intergenic	20.0%
!!	AAAAAGGAAAGAAACTAATT+GGG	-	chr1.3:48225737-48225756	None:intergenic	20.0%
!!	AAAATTAGGCAAAGAAAAAA+TGG	-	chr1.3:48225864-48225883	None:intergenic	20.0%
!!	AAATTAGGCAAAGAAAAAAT+GGG	-	chr1.3:48225863-48225882	None:intergenic	20.0%
!!	AGCTAAAAAGAAATAAATGA+AGG	+	chr1.3:48225412-48225431	MS.gene24050:intron	20.0%
!!	ATTGTATTTAATTATCACAG+GGG	+	chr1.3:48224950-48224969	MS.gene24050:intron	20.0%
!!	GAAAATTTGTATTTGTTTCA+CGG	+	chr1.3:48225104-48225123	MS.gene24050:intron	20.0%
!!	GCTAAAAAGAAATAAATGAA+GGG	+	chr1.3:48225413-48225432	MS.gene24050:intron	20.0%
!!	TATACCAATTAATGCAAAAA+AGG	-	chr1.3:48225753-48225772	None:intergenic	20.0%
!!!	ATTTGGATTATTTTGTTCAT+GGG	+	chr1.3:48225050-48225069	MS.gene24050:intron	20.0%
!!!	CATTTTTTTGATTTGGATTT+CGG	-	chr1.3:48224201-48224220	None:intergenic	20.0%
!!!	TATTTGGATTATTTTGTTCA+TGG	+	chr1.3:48225049-48225068	MS.gene24050:intron	20.0%
!!!	TGTATTGATAATGTATTTGT+GGG	+	chr1.3:48225468-48225487	MS.gene24050:intron	20.0%
!!!	TTCATTTATTTCTTTTTAGC+TGG	-	chr1.3:48225413-48225432	None:intergenic	20.0%
!	CAACAAATTGAACTAATACA+TGG	-	chr1.3:48225153-48225172	None:intergenic	25.0%
!	CGAAATCCAAATCAAAAAAA+TGG	+	chr1.3:48224199-48224218	MS.gene24050:CDS	25.0%
!	TGACTGCATTAAAAAGATAA+CGG	-	chr1.3:48225374-48225393	None:intergenic	25.0%
!	TGATTACATTCAATTACTAC+CGG	+	chr1.3:48224849-48224868	MS.gene24050:intron	25.0%
!	TTGAATGTAATCAAATGTGT+CGG	-	chr1.3:48224842-48224861	None:intergenic	25.0%
!!	CTATTTGATAACACTTATCT+CGG	-	chr1.3:48225264-48225283	None:intergenic	25.0%
!!	GAAAAATAATCATTTTGCTG+TGG	-	chr1.3:48224731-48224750	None:intergenic	25.0%
!!!	CTTTCCTTTTTTGCATTAAT+TGG	+	chr1.3:48225746-48225765	MS.gene24050:intron	25.0%
!!!	GGATTTGATAACTTTTATGA+AGG	+	chr1.3:48226424-48226443	MS.gene24050:CDS	25.0%
!!!	GTGTATTGATAATGTATTTG+TGG	+	chr1.3:48225467-48225486	MS.gene24050:intron	25.0%
!!!	TGATTTTATTTCAATTGCAC+TGG	-	chr1.3:48225697-48225716	None:intergenic	25.0%
!!!	TTATTTTTCTGTTGTTTCAC+AGG	+	chr1.3:48224742-48224761	MS.gene24050:CDS	25.0%
!!!	TTTGGATTATTTTGTTCATG+GGG	+	chr1.3:48225051-48225070	MS.gene24050:intron	25.0%
!!!	TTTTTTTTGCTCTTTGTTTC+AGG	+	chr1.3:48225889-48225908	MS.gene24050:intron	25.0%
	ACTATCAGGATTTCAATCAA+GGG	+	chr1.3:48226113-48226132	MS.gene24050:CDS	30.0%
	ATTTAATTATCACAGGGGAT+TGG	+	chr1.3:48224955-48224974	MS.gene24050:intron	30.0%
	CAAATCAAAAAAATGGAAGC+TGG	+	chr1.3:48224206-48224225	MS.gene24050:CDS	30.0%
	CAAATTCGACTACTAATAAC+TGG	+	chr1.3:48226062-48226081	MS.gene24050:CDS	30.0%
	CACAAAAGAGATTACTTCTA+AGG	+	chr1.3:48225026-48225045	MS.gene24050:CDS	30.0%
	CATGCTTTATAGTTGATTAG+TGG	+	chr1.3:48224902-48224921	MS.gene24050:intron	30.0%
	CGAAATGAGAATGATAGAAA+TGG	+	chr1.3:48226088-48226107	MS.gene24050:CDS	30.0%
	TACTATCAGGATTTCAATCA+AGG	+	chr1.3:48226112-48226131	MS.gene24050:CDS	30.0%
!	AAGGAGAATTTAGGTTCAAA+GGG	+	chr1.3:48224383-48224402	MS.gene24050:CDS	30.0%
!	TATGACTTTTAGCATAGTGT+GGG	+	chr1.3:48225628-48225647	MS.gene24050:intron	30.0%
!	TTATGACTTTTAGCATAGTG+TGG	+	chr1.3:48225627-48225646	MS.gene24050:intron	30.0%
!!	GAGATTACTTCTAAGGTATT+TGG	+	chr1.3:48225033-48225052	MS.gene24050:intron	30.0%
!!	GGGAATTGATAATGTTAACT+TGG	+	chr1.3:48225488-48225507	MS.gene24050:intron	30.0%
!!!	CAGCTTCCATTTTTTTGATT+TGG	-	chr1.3:48224208-48224227	None:intergenic	30.0%
	AAACTCAAAGCTCAAACTCT+TGG	+	chr1.3:48226191-48226210	MS.gene24050:CDS	35.0%
	AAAGGAGGTAATTCTTCTAC+TGG	+	chr1.3:48226139-48226158	MS.gene24050:CDS	35.0%
	AACTCAAAGCTCAAACTCTT+GGG	+	chr1.3:48226192-48226211	MS.gene24050:CDS	35.0%
	AAGGGAGAACCTACTAATAA+AGG	+	chr1.3:48226241-48226260	MS.gene24050:CDS	35.0%
	ACAGTGGATGTTAATTGGAT+CGG	+	chr1.3:48225589-48225608	MS.gene24050:intron	35.0%
	ACATGGTAGAAATATCCCTA+AGG	-	chr1.3:48225136-48225155	None:intergenic	35.0%
	ACTCAAAAGACATCAGAGAT+TGG	+	chr1.3:48225962-48225981	MS.gene24050:CDS	35.0%
	AGTCATATTCATATCTACGG+AGG	-	chr1.3:48224819-48224838	None:intergenic	35.0%
	ATCAAAAAAATGGAAGCTGG+TGG	+	chr1.3:48224209-48224228	MS.gene24050:CDS	35.0%
	CATGACTCGTAGAAAAACAA+AGG	-	chr1.3:48224787-48224806	None:intergenic	35.0%
	CGGAGTCATATTCATATCTA+CGG	-	chr1.3:48224822-48224841	None:intergenic	35.0%
	GAAGAGAACAATCTTCATCA+GGG	+	chr1.3:48226161-48226180	MS.gene24050:CDS	35.0%
	GATTTCAATCAAGGGAACAA+AGG	+	chr1.3:48226121-48226140	MS.gene24050:CDS	35.0%
	GTAACAATATCCGTTTCCTT+CGG	-	chr1.3:48224275-48224294	None:intergenic	35.0%
	GTATTCTATTCCGAAGGAAA+CGG	+	chr1.3:48224262-48224281	MS.gene24050:CDS	35.0%
	TCAGAACAGTGGATGTTAAT+TGG	+	chr1.3:48225584-48225603	MS.gene24050:intron	35.0%
	TCATCTAATAAGAAAGCTGC+TGG	+	chr1.3:48226448-48226467	MS.gene24050:CDS	35.0%
	TGATAGAAATGGCTACTATC+AGG	+	chr1.3:48226099-48226118	MS.gene24050:CDS	35.0%
	TGATGTGTGATTGACAATAC+TGG	+	chr1.3:48225344-48225363	MS.gene24050:intron	35.0%
	TGGGATAATGCAAAAGATGA+TGG	+	chr1.3:48226403-48226422	MS.gene24050:CDS	35.0%
	TTCCTTCGGAATAGAATACT+TGG	-	chr1.3:48224261-48224280	None:intergenic	35.0%
!	AGAATTTAGGTTCAAAGGGA+AGG	+	chr1.3:48224387-48224406	MS.gene24050:CDS	35.0%
!	ATGACTTTTAGCATAGTGTG+GGG	+	chr1.3:48225629-48225648	MS.gene24050:intron	35.0%
!	GAAGGAGAATTTAGGTTCAA+AGG	+	chr1.3:48224382-48224401	MS.gene24050:CDS	35.0%
!	GGAGTTGATCCTTTATTAGT+AGG	-	chr1.3:48226253-48226272	None:intergenic	35.0%
!!	GATTTTAGGAATTTGAACGG+CGG	+	chr1.3:48224509-48224528	MS.gene24050:CDS	35.0%
!!	GGGGATTTTAGGAATTTGAA+CGG	+	chr1.3:48224506-48224525	MS.gene24050:CDS	35.0%
!!	TGCTAACTGTTTGCTTTTCA+CGG	+	chr1.3:48225824-48225843	MS.gene24050:intron	35.0%
!!!	ATTTTTTGCGAAGAAGATGG+CGG	+	chr1.3:48224613-48224632	MS.gene24050:CDS	35.0%
!!!	TAGCTGTTTTTCGAATTCCA+CGG	-	chr1.3:48225220-48225239	None:intergenic	35.0%
	AAGAAAGCTGCTGGACATAA+TGG	+	chr1.3:48226457-48226476	MS.gene24050:CDS	40.0%
	AAGGACATGGGGTTTAATGA+AGG	+	chr1.3:48225988-48226007	MS.gene24050:CDS	40.0%
	AGAAAGCTGCTGGACATAAT+GGG	+	chr1.3:48226458-48226477	MS.gene24050:CDS	40.0%
	AGCTGTTGAATGAGGTCAAT+AGG	+	chr1.3:48225660-48225679	MS.gene24050:intron	40.0%
	AGGACATGGGGTTTAATGAA+GGG	+	chr1.3:48225989-48226008	MS.gene24050:CDS	40.0%
	AGTACCTTCATGATTGTCTC+TGG	+	chr1.3:48225787-48225806	MS.gene24050:intron	40.0%
	AGTTTATCGTGATCGGATTC+AGG	+	chr1.3:48224319-48224338	MS.gene24050:CDS	40.0%
	ATTGGGACCACAAAGATTCT+AGG	+	chr1.3:48226323-48226342	MS.gene24050:CDS	40.0%
	ATTGGGGCCAAAAAGATAGT+TGG	+	chr1.3:48226218-48226237	MS.gene24050:CDS	40.0%
	CAACCAGCATCATTATTGTG+GGG	-	chr1.3:48226376-48226395	None:intergenic	40.0%
	CAACTCCTTATTCATCTGGT+GGG	+	chr1.3:48226266-48226285	MS.gene24050:CDS	40.0%
	CCAACCAGCATCATTATTGT+GGG	-	chr1.3:48226377-48226396	None:intergenic	40.0%
	CCTAAATTCTCCTTCACAAC+CGG	-	chr1.3:48224377-48224396	None:intergenic	40.0%
	CTAGTATGCTATAGCACTAC+AGG	-	chr1.3:48225294-48225313	None:intergenic	40.0%
	CTGCTTCAGTTTATCGTGAT+CGG	+	chr1.3:48224312-48224331	MS.gene24050:CDS	40.0%
	CTTCCTTCCTAGAATCTTTG+TGG	-	chr1.3:48226333-48226352	None:intergenic	40.0%
	GCTATCTGATAACACTGTAG+TGG	-	chr1.3:48225189-48225208	None:intergenic	40.0%
	GCTGTTGAATGAGGTCAATA+GGG	+	chr1.3:48225661-48225680	MS.gene24050:intron	40.0%
	GGAAGAGAACAATCTTCATC+AGG	+	chr1.3:48226160-48226179	MS.gene24050:CDS	40.0%
	GGATCAACTCCTTATTCATC+TGG	+	chr1.3:48226262-48226281	MS.gene24050:CDS	40.0%
	GGCCAAAAAGATAGTTGGAA+GGG	+	chr1.3:48226223-48226242	MS.gene24050:CDS	40.0%
	TAGAGGAAAGCTGTTGAATG+AGG	+	chr1.3:48225652-48225671	MS.gene24050:intron	40.0%
	TATTTATTCGCGGTCGCAAT+TGG	+	chr1.3:48224565-48224584	MS.gene24050:CDS	40.0%
	TCAACTCCTTATTCATCTGG+TGG	+	chr1.3:48226265-48226284	MS.gene24050:CDS	40.0%
	TCGGAATAGAATACTTGGCA+AGG	-	chr1.3:48224256-48224275	None:intergenic	40.0%
	TGCCAAGTATTCTATTCCGA+AGG	+	chr1.3:48224256-48224275	MS.gene24050:CDS	40.0%
	TTCAATCAAGGGAACAAAGG+AGG	+	chr1.3:48226124-48226143	MS.gene24050:CDS	40.0%
	TTCATGTGCTTAGAATGCTC+CGG	+	chr1.3:48224113-48224132	MS.gene24050:CDS	40.0%
!	AGACATCAGAGATTGGTCAA+AGG	+	chr1.3:48225969-48225988	MS.gene24050:CDS	40.0%
!	AGAGATTGGTCAAAGGACAT+GGG	+	chr1.3:48225976-48225995	MS.gene24050:CDS	40.0%
!	CCACAATAATGATGCTGGTT+GGG	+	chr1.3:48226375-48226394	MS.gene24050:CDS	40.0%
!	CTCCCTTCCAACTATCTTTT+TGG	-	chr1.3:48226228-48226247	None:intergenic	40.0%
!	TCTTTGTTTCAGGTCAAGGA+AGG	+	chr1.3:48225899-48225918	MS.gene24050:intron	40.0%
!	TTGCTCTTTGTTTCAGGTCA+AGG	+	chr1.3:48225895-48225914	MS.gene24050:intron	40.0%
!!	GCTTTTCACGGTATTTTTGC+TGG	+	chr1.3:48225836-48225855	MS.gene24050:intron	40.0%
!!!	GGGATTTTTTGCGAAGAAGA+TGG	+	chr1.3:48224610-48224629	MS.gene24050:CDS	40.0%
	AAATCAGATGACGCTTGGAC+AGG	+	chr1.3:48226481-48226500	MS.gene24050:CDS	45.0%
	AAATTCTCCTTCACAACCGG+AGG	-	chr1.3:48224374-48224393	None:intergenic	45.0%
	AATCAGATGACGCTTGGACA+GGG	+	chr1.3:48226482-48226501	MS.gene24050:CDS	45.0%
	ACGATAAACTGAAGCAGCGT+TGG	-	chr1.3:48224309-48224328	None:intergenic	45.0%
	ATGGGAAATCAGATGACGCT+TGG	+	chr1.3:48226476-48226495	MS.gene24050:CDS	45.0%
	CCCAACCAGCATCATTATTG+TGG	-	chr1.3:48226378-48226397	None:intergenic	45.0%
	CCCACAATAATGATGCTGGT+TGG	+	chr1.3:48226374-48226393	MS.gene24050:CDS	45.0%
	CCGGTTGTGAAGGAGAATTT+AGG	+	chr1.3:48224374-48224393	MS.gene24050:CDS	45.0%
	CGACGGAGGATATTTATTCG+CGG	+	chr1.3:48224555-48224574	MS.gene24050:CDS	45.0%
	CTCAAACTCTTGGGACGATT+GGG	+	chr1.3:48226201-48226220	MS.gene24050:CDS	45.0%
	CTCTCAGAAGAATCCACAGT+GGG	+	chr1.3:48224070-48224089	MS.gene24050:CDS	45.0%
	GACATGGGGTTTAATGAAGG+GGG	+	chr1.3:48225991-48226010	MS.gene24050:CDS	45.0%
	GATGCCAGAGACAATCATGA+AGG	-	chr1.3:48225794-48225813	None:intergenic	45.0%
	GGAAGGCGGTTATGATCAAA+AGG	+	chr1.3:48225916-48225935	MS.gene24050:CDS	45.0%
	GGACATGGGGTTTAATGAAG+GGG	+	chr1.3:48225990-48226009	MS.gene24050:CDS	45.0%
	GGGCCAAAAAGATAGTTGGA+AGG	+	chr1.3:48226222-48226241	MS.gene24050:CDS	45.0%
	GTTTCACGGAATGCACCTTA+GGG	+	chr1.3:48225118-48225137	MS.gene24050:intron	45.0%
	TAAGCACATGAAGATGCCGT+AGG	-	chr1.3:48224105-48224124	None:intergenic	45.0%
	TACTCACAATCCCAGCTCTT+GGG	+	chr1.3:48226297-48226316	MS.gene24050:CDS	45.0%
	TCAAACTCTTGGGACGATTG+GGG	+	chr1.3:48226202-48226221	MS.gene24050:CDS	45.0%
	TCAGATAGCTGATATAGCCG+TGG	+	chr1.3:48225200-48225219	MS.gene24050:intron	45.0%
	TCTCGGCATATCACAAATCG+TGG	-	chr1.3:48225247-48225266	None:intergenic	45.0%
	TGATGTGAGGAGTAAGTCGA+CGG	+	chr1.3:48224484-48224503	MS.gene24050:CDS	45.0%
	TGTTTCACGGAATGCACCTT+AGG	+	chr1.3:48225117-48225136	MS.gene24050:intron	45.0%
	TTTAGCATAGTGTGGGGTAG+AGG	+	chr1.3:48225635-48225654	MS.gene24050:intron	45.0%
	TTTAGGAATTTGAACGGCGG+TGG	+	chr1.3:48224512-48224531	MS.gene24050:CDS	45.0%
!	AATAATGATGCTGGTTGGGC+TGG	+	chr1.3:48226379-48226398	MS.gene24050:CDS	45.0%
!	CAGAGATTGGTCAAAGGACA+TGG	+	chr1.3:48225975-48225994	MS.gene24050:CDS	45.0%
!	GAGATTGGTCAAAGGACATG+GGG	+	chr1.3:48225977-48225996	MS.gene24050:CDS	45.0%
!	GGACCACAAAGATTCTAGGA+AGG	+	chr1.3:48226327-48226346	MS.gene24050:CDS	45.0%
!	GGGGATTGGTAAGTTGTCAT+TGG	+	chr1.3:48224969-48224988	MS.gene24050:intron	45.0%
!	TGATCGGATTCAGGCTTTGT+CGG	+	chr1.3:48224328-48224347	MS.gene24050:CDS	45.0%
!	TGTGGATTGCTCCTTTGTGA+AGG	-	chr1.3:48224713-48224732	None:intergenic	45.0%
!	TTGCTCCTTTGTGAAGGCAT+CGG	-	chr1.3:48224707-48224726	None:intergenic	45.0%
!	TTGTTTCAGGTCAAGGAAGG+CGG	+	chr1.3:48225902-48225921	MS.gene24050:intron	45.0%
!	TTTGGCTTCACAGAAGGTTG+AGG	+	chr1.3:48226021-48226040	MS.gene24050:CDS	45.0%
!!	AGCAGCGTTGGTGTTATACT+TGG	-	chr1.3:48224297-48224316	None:intergenic	45.0%
!!	CAAAAAAAAAAAAAAAAATT+AGG	-	chr1.3:48225878-48225897	None:intergenic	5.0%
	AATGAAGGGGGTCATGGCTT+TGG	+	chr1.3:48226003-48226022	MS.gene24050:CDS	50.0%
	ACATACTTCCCACCTTGCGA+CGG	-	chr1.3:48224668-48224687	None:intergenic	50.0%
	AGATGTGAACACCGAGACCA+CGG	-	chr1.3:48224145-48224164	None:intergenic	50.0%
	AGTCGCCGGAGTAATGATTC+CGG	+	chr1.3:48224425-48224444	MS.gene24050:CDS	50.0%
	ATCAGATGACGCTTGGACAG+GGG	+	chr1.3:48226483-48226502	MS.gene24050:CDS	50.0%
	ATCCCAGCTCTTGGGATGAT+TGG	+	chr1.3:48226305-48226324	MS.gene24050:CDS	50.0%
	ATCGACGCAGATCTTGTTGC+CGG	-	chr1.3:48224048-48224067	None:intergenic	50.0%
	ATGATTCCGGCTGGGATGAT+TGG	+	chr1.3:48224438-48224457	MS.gene24050:CDS	50.0%
	ATGTGAGGAGTAAGTCGACG+GGG	+	chr1.3:48224486-48224505	MS.gene24050:CDS	50.0%
	CTAGTCCGATGCCTTCACAA+AGG	+	chr1.3:48224699-48224718	MS.gene24050:CDS	50.0%
	GACTGCCCACCAGATGAATA+AGG	-	chr1.3:48226274-48226293	None:intergenic	50.0%
	GATGATTGGGATAGGGAAGG+AGG	+	chr1.3:48224452-48224471	MS.gene24050:CDS	50.0%
	GATGTGAGGAGTAAGTCGAC+GGG	+	chr1.3:48224485-48224504	MS.gene24050:CDS	50.0%
	GATTTCGGACCAAGCATCCA+TGG	-	chr1.3:48224186-48224205	None:intergenic	50.0%
	GCTCAAACTCTTGGGACGAT+TGG	+	chr1.3:48226200-48226219	MS.gene24050:CDS	50.0%
	GCTCCCCACAATAATGATGC+TGG	+	chr1.3:48226370-48226389	MS.gene24050:CDS	50.0%
	GCTCTCAGAAGAATCCACAG+TGG	+	chr1.3:48224069-48224088	MS.gene24050:CDS	50.0%
	GCTGCTAATGCTGTTCAGAC+TGG	+	chr1.3:48225003-48225022	MS.gene24050:CDS	50.0%
	GGGTTTAATGAAGGGGGTCA+TGG	+	chr1.3:48225997-48226016	MS.gene24050:CDS	50.0%
	GTACTCACAATCCCAGCTCT+TGG	+	chr1.3:48226296-48226315	MS.gene24050:CDS	50.0%
	GTCGCAATTGGAAGCTTCTG+CGG	+	chr1.3:48224577-48224596	MS.gene24050:CDS	50.0%
	TCCCAATCATCCCAAGAGCT+GGG	-	chr1.3:48226310-48226329	None:intergenic	50.0%
	TCCCAGCTCTTGGGATGATT+GGG	+	chr1.3:48226306-48226325	MS.gene24050:CDS	50.0%
	TGATTCCGGCTGGGATGATT+GGG	+	chr1.3:48224439-48224458	MS.gene24050:CDS	50.0%
	TGGGATGATTGGGATAGGGA+AGG	+	chr1.3:48224449-48224468	MS.gene24050:CDS	50.0%
	TTGGGATAGGGAAGGAGGTT+CGG	+	chr1.3:48224457-48224476	MS.gene24050:CDS	50.0%
!	ATCCACTGTTCTGAGCAACG+AGG	-	chr1.3:48225578-48225597	None:intergenic	50.0%
!	CGCAAGGTGGGAAGTATGTT+GGG	+	chr1.3:48224669-48224688	MS.gene24050:CDS	50.0%
!	GGTGGGAAGTATGTTGGGTT+CGG	+	chr1.3:48224674-48224693	MS.gene24050:CDS	50.0%
!	GTAAGTCGACGGGGGATTTT+AGG	+	chr1.3:48224495-48224514	MS.gene24050:CDS	50.0%
!	TATTCATCTGGTGGGCAGTC+TGG	+	chr1.3:48226274-48226293	MS.gene24050:CDS	50.0%
!	TCGCAAGGTGGGAAGTATGT+TGG	+	chr1.3:48224668-48224687	MS.gene24050:CDS	50.0%
!	TTCAGGCTTTGTCGGAAGGT+CGG	+	chr1.3:48224336-48224355	MS.gene24050:CDS	50.0%
!!	AAGCATCCATGGTGACGGAT+CGG	-	chr1.3:48224175-48224194	None:intergenic	50.0%
!!	AGATGGCGGAGAATGAGTCA+AGG	+	chr1.3:48224627-48224646	MS.gene24050:CDS	50.0%
!!	CATGGCTTTGGCTTCACAGA+AGG	+	chr1.3:48226015-48226034	MS.gene24050:CDS	50.0%
	AGCATGCACACACACACACC+CGG	-	chr1.3:48224888-48224907	None:intergenic	55.0%
	CAATTGGAAGCTTCTGCGGC+GGG	+	chr1.3:48224581-48224600	MS.gene24050:CDS	55.0%
	CAGTGGGCATCAGTCTCCTA+CGG	+	chr1.3:48224086-48224105	MS.gene24050:CDS	55.0%
	CCCAGCCGGAATCATTACTC+CGG	-	chr1.3:48224433-48224452	None:intergenic	55.0%
	CCGGAGTAATGATTCCGGCT+GGG	+	chr1.3:48224430-48224449	MS.gene24050:CDS	55.0%
	CCTATCCCAATCATCCCAGC+CGG	-	chr1.3:48224447-48224466	None:intergenic	55.0%
	CGGCTGGGATGATTGGGATA+GGG	+	chr1.3:48224445-48224464	MS.gene24050:CDS	55.0%
	GAATGCTCCGGAAAACACCG+TGG	+	chr1.3:48224125-48224144	MS.gene24050:CDS	55.0%
	GAGAATGAGTCAAGGCCGGA+AGG	+	chr1.3:48224635-48224654	MS.gene24050:CDS	55.0%
	GATCCGTCACCATGGATGCT+TGG	+	chr1.3:48224174-48224193	MS.gene24050:CDS	55.0%
	GCAATTGGAAGCTTCTGCGG+CGG	+	chr1.3:48224580-48224599	MS.gene24050:CDS	55.0%
	GCAGATCTTGTTGCCGGGTT+GGG	-	chr1.3:48224042-48224061	None:intergenic	55.0%
	GGACCAAGCATCCATGGTGA+CGG	-	chr1.3:48224180-48224199	None:intergenic	55.0%
	GTCCCAATCATCCCAAGAGC+TGG	-	chr1.3:48226311-48226330	None:intergenic	55.0%
	TAGGAGACTGATGCCCACTG+TGG	-	chr1.3:48224086-48224105	None:intergenic	55.0%
	TCAGATGACGCTTGGACAGG+GGG	+	chr1.3:48226484-48226503	MS.gene24050:CDS	55.0%
	TCCGGAAAACACCGTGGTCT+CGG	+	chr1.3:48224131-48224150	MS.gene24050:CDS	55.0%
	TCGACGCAGATCTTGTTGCC+GGG	-	chr1.3:48224047-48224066	None:intergenic	55.0%
	TGCCTCGTTGCTCAGAACAG+TGG	+	chr1.3:48225573-48225592	MS.gene24050:intron	55.0%
	TGGGATAGGGAAGGAGGTTC+GGG	+	chr1.3:48224458-48224477	MS.gene24050:CDS	55.0%
	TGGGGAGCTGATGCTTTAGC+TGG	-	chr1.3:48226358-48226377	None:intergenic	55.0%
	TGTGAGGAGTAAGTCGACGG+GGG	+	chr1.3:48224487-48224506	MS.gene24050:CDS	55.0%
!	ACCGAGACCACGGTGTTTTC+CGG	-	chr1.3:48224135-48224154	None:intergenic	55.0%
!	CGGATTCAGGCTTTGTCGGA+AGG	+	chr1.3:48224332-48224351	MS.gene24050:CDS	55.0%
!	TACCGGTGTCAGTGTTGTGC+CGG	+	chr1.3:48224866-48224885	MS.gene24050:intron	55.0%
!	TGTTGCCGGGTTGGGATTGA+AGG	-	chr1.3:48224034-48224053	None:intergenic	55.0%
	ACCCGGCACAACACTGACAC+CGG	-	chr1.3:48224871-48224890	None:intergenic	60.0%
	AGCTTCTGCGGCGGGTAAAG+AGG	+	chr1.3:48224589-48224608	MS.gene24050:CDS	60.0%
	CCGGCTGGGATGATTGGGAT+AGG	+	chr1.3:48224444-48224463	MS.gene24050:CDS	60.0%
	CGCAGATCTTGTTGCCGGGT+TGG	-	chr1.3:48224043-48224062	None:intergenic	60.0%
	CGGGACCTTCAATCCCAACC+CGG	+	chr1.3:48224026-48224045	MS.gene24050:CDS	60.0%
	CTTCGTCCGATCCGTCACCA+TGG	+	chr1.3:48224166-48224185	MS.gene24050:CDS	60.0%
	CTTTGTCGGAAGGTCGGTCG+TGG	+	chr1.3:48224342-48224361	MS.gene24050:CDS	60.0%
	GAAGGACTTCCACCGTCGCA+AGG	+	chr1.3:48224653-48224672	MS.gene24050:CDS	60.0%
	GACTTCCACCGTCGCAAGGT+GGG	+	chr1.3:48224657-48224676	MS.gene24050:CDS	60.0%
	GATAGGGAAGGAGGTTCGGG+TGG	+	chr1.3:48224461-48224480	MS.gene24050:CDS	60.0%
	GCCGGAGTAATGATTCCGGC+TGG	+	chr1.3:48224429-48224448	MS.gene24050:CDS	60.0%
	GCGTGATCCTCCGGTTGTGA+AGG	+	chr1.3:48224364-48224383	MS.gene24050:CDS	60.0%
	GCTTCTGCGGCGGGTAAAGA+GGG	+	chr1.3:48224590-48224609	MS.gene24050:CDS	60.0%
	GGCGGAGAATGAGTCAAGGC+CGG	+	chr1.3:48224631-48224650	MS.gene24050:CDS	60.0%
	TACTTCCCACCTTGCGACGG+TGG	-	chr1.3:48224665-48224684	None:intergenic	60.0%
	TCAAAGGGAAGGCCGCCGAT+GGG	+	chr1.3:48224398-48224417	MS.gene24050:CDS	60.0%
	TGCGACGGTGGAAGTCCTTC+CGG	-	chr1.3:48224653-48224672	None:intergenic	60.0%
	TTCAAAGGGAAGGCCGCCGA+TGG	+	chr1.3:48224397-48224416	MS.gene24050:CDS	60.0%
	TTTGAACGGCGGTGGTGCAC+CGG	+	chr1.3:48224520-48224539	MS.gene24050:CDS	60.0%
!	ACCGGTGTCAGTGTTGTGCC+GGG	+	chr1.3:48224867-48224886	MS.gene24050:intron	60.0%
!	CGGGTTGGGATTGAAGGTCC+CGG	-	chr1.3:48224028-48224047	None:intergenic	60.0%
!	GAGGTTCGGGTGGTGATGTG+AGG	+	chr1.3:48224471-48224490	MS.gene24050:CDS	60.0%
!!	ATGATGCTGGTTGGGCTGGC+TGG	+	chr1.3:48226383-48226402	MS.gene24050:CDS	60.0%
!!	CATGGTGACGGATCGGACGA+AGG	-	chr1.3:48224168-48224187	None:intergenic	60.0%
!!	TGATGCTGGTTGGGCTGGCT+GGG	+	chr1.3:48226384-48226403	MS.gene24050:CDS	60.0%
	ACCGGCGAGATCGAGGTCGA+CGG	+	chr1.3:48224538-48224557	MS.gene24050:CDS	65.0%
	CAAAGGGAAGGCCGCCGATG+GGG	+	chr1.3:48224399-48224418	MS.gene24050:CDS	65.0%
	GATGACGCTTGGACAGGGGG+TGG	+	chr1.3:48226487-48226506	MS.gene24050:CDS	65.0%
	GGACTTCCACCGTCGCAAGG+TGG	+	chr1.3:48224656-48224675	MS.gene24050:CDS	65.0%
	TCCGTCGACCTCGATCTCGC+CGG	-	chr1.3:48224542-48224561	None:intergenic	65.0%
	TCGGTCGTGGCGTGATCCTC+CGG	+	chr1.3:48224355-48224374	MS.gene24050:CDS	65.0%
	CTCCGGCGACTCTGCCCCAT+CGG	-	chr1.3:48224416-48224435	None:intergenic	70.0%
	GGCGAGATCGAGGTCGACGG+AGG	+	chr1.3:48224541-48224560	MS.gene24050:CDS	70.0%
!	GTGGTGCACCGGCGAGATCG+AGG	+	chr1.3:48224531-48224550	MS.gene24050:CDS	70.0%
	CGCCGATGGGGCAGAGTCGC+CGG	+	chr1.3:48224411-48224430	MS.gene24050:CDS	75.0%
	CGGCGACTCTGCCCCATCGG+CGG	-	chr1.3:48224413-48224432	None:intergenic	75.0%
	TGGCGGCATCACGGCGGCTC+CGG	+	chr1.3:48224006-48224025	MS.gene24050:CDS	75.0%
!	GGCGGCATCACGGCGGCTCC+GGG	+	chr1.3:48224007-48224026	MS.gene24050:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	48224005	48226519	48224005	ID=MS.gene24050
chr1.3	mRNA	48224005	48226519	48224005	ID=MS.gene24050.t1;Parent=MS.gene24050
chr1.3	exon	48224005	48224763	48224005	ID=MS.gene24050.t1.exon1;Parent=MS.gene24050.t1
chr1.3	CDS	48224005	48224763	48224005	ID=cds.MS.gene24050.t1;Parent=MS.gene24050.t1
chr1.3	exon	48224970	48225047	48224970	ID=MS.gene24050.t1.exon2;Parent=MS.gene24050.t1
chr1.3	CDS	48224970	48225047	48224970	ID=cds.MS.gene24050.t1;Parent=MS.gene24050.t1
chr1.3	exon	48225911	48226519	48225911	ID=MS.gene24050.t1.exon3;Parent=MS.gene24050.t1
chr1.3	CDS	48225911	48226519	48225911	ID=cds.MS.gene24050.t1;Parent=MS.gene24050.t1

Gene Sequence

>MS.gene24050

ATGGCGGCATCACGGCGGCTCCGGGACCTTCAATCCCAACCCGGCAACAAGATCTGCGTCGATTGCTCTCAGAAGAATCCACAGTGGGCATCAGTCTCCTACGGCATCTTCATGTGCTTAGAATGCTCCGGAAAACACCGTGGTCTCGGTGTTCACATCTCCTTCGTCCGATCCGTCACCATGGATGCTTGGTCCGAAATCCAAATCAAAAAAATGGAAGCTGGTGGTAATGACAATCTCAACGCTTTCCTTGCCAAGTATTCTATTCCGAAGGAAACGGATATTGTTACCAAGTATAACACCAACGCTGCTTCAGTTTATCGTGATCGGATTCAGGCTTTGTCGGAAGGTCGGTCGTGGCGTGATCCTCCGGTTGTGAAGGAGAATTTAGGTTCAAAGGGAAGGCCGCCGATGGGGCAGAGTCGCCGGAGTAATGATTCCGGCTGGGATGATTGGGATAGGGAAGGAGGTTCGGGTGGTGATGTGAGGAGTAAGTCGACGGGGGATTTTAGGAATTTGAACGGCGGTGGTGCACCGGCGAGATCGAGGTCGACGGAGGATATTTATTCGCGGTCGCAATTGGAAGCTTCTGCGGCGGGTAAAGAGGGATTTTTTGCGAAGAAGATGGCGGAGAATGAGTCAAGGCCGGAAGGACTTCCACCGTCGCAAGGTGGGAAGTATGTTGGGTTCGGATCTAGTCCGATGCCTTCACAAAGGAGCAATCCACAGCAAAATGATTATTTTTCTGTTGTTTCACAGGTGATTGAATTGTTTGAGTTCCTTTGTTTTTCTACGAGTCATGAATTGATTACCTCCGTAGATATGAATATGACTCCGACACATTTGATTACATTCAATTACTACCGGTGTCAGTGTTGTGCCGGGTGTGTGTGTGTGCATGCTTTATAGTTGATTAGTGGTCTGTGTTTGCTTATTTGTTTAATTATTGTATTTAATTATCACAGGGGATTGGTAAGTTGTCATTGGTTGCTGCATCTGCTGCTAATGCTGTTCAGACTGGCACAAAAGAGATTACTTCTAAGGTATTTGGATTATTTTGTTCATGGGGTGAGATGATTGTTATTGATATTTTGATGATGAAAATTTGTATTTGTTTCACGGAATGCACCTTAGGGATATTTCTACCATGTATTAGTTCAATTTGTTGCTTCTTCTATAGTCCACTACAGTGTTATCAGATAGCTGATATAGCCGTGGAATTCGAAAAACAGCTATTGTCCACGATTTGTGATATGCCGAGATAAGTGTTATCAAATAGACTACAGCCTGTAGTGCTATAGCATACTAGTAGAATTTGAACAAATGTTTATGTCTGCTATGATGTGTGATTGACAATACTGGTTTACCGTTATCTTTTTAATGCAGTCATTCATTTGTCGCTTTGCCAGCTAAAAAGAAATAAATGAAGGGAGAAATGAGACAAAAATGAATGTTTTTCTTTGTGTATTGATAATGTATTTGTGGGAATTGATAATGTTAACTTGGCGTTTAAGAAGTGTGAATAAAATATTTGTTTTGTGTATAATTTTCATTTCTTCATTTAGTTTTGCCTCGTTGCTCAGAACAGTGGATGTTAATTGGATCGGAATTTTCAAATGTAGTTATGACTTTTAGCATAGTGTGGGGTAGAGGAAAGCTGTTGAATGAGGTCAATAGGGAACAAAGTGTCCAGTGCAATTGAAATAAAATCATAAAACAACATATCTCTCCCAATTAGTTTCTTTCCTTTTTTGCATTAATTGGTATATTAGTAAAAGAAAAAGTACCTTCATGATTGTCTCTGGCATCGAGATACGTCTGCTAACTGTTTGCTTTTCACGGTATTTTTGCTGGTCCCATTTTTTCTTTGCCTAATTTTTTTTTTTTTTTTTGCTCTTTGTTTCAGGTCAAGGAAGGCGGTTATGATCAAAAGGTCAACGAAACTGTCACTGTTGTCACTCAAAAGACATCAGAGATTGGTCAAAGGACATGGGGTTTAATGAAGGGGGTCATGGCTTTGGCTTCACAGAAGGTTGAGGAGTACACGAAAGAAAACTCAAATTCGACTACTAATAACTGGCAACGAAATGAGAATGATAGAAATGGCTACTATCAGGATTTCAATCAAGGGAACAAAGGAGGTAATTCTTCTACTGGAAGAGAACAATCTTCATCAGGGCAATACAAAACTCAAAGCTCAAACTCTTGGGACGATTGGGGCCAAAAAGATAGTTGGAAGGGAGAACCTACTAATAAAGGATCAACTCCTTATTCATCTGGTGGGCAGTCTGGTACTCACAATCCCAGCTCTTGGGATGATTGGGACCACAAAGATTCTAGGAAGGAAGAACCAGCTAAAGCATCAGCTCCCCACAATAATGATGCTGGTTGGGCTGGCTGGGATAATGCAAAAGATGATGGATTTGATAACTTTTATGAAGGTTCATCTAATAAGAAAGCTGCTGGACATAATGGGAAATCAGATGACGCTTGGACAGGGGGTGGCTTTCTCTAA

Protein sequence

>MS.gene24050.t1

MAASRRLRDLQSQPGNKICVDCSQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVTMDAWSEIQIKKMEAGGNDNLNAFLAKYSIPKETDIVTKYNTNAASVYRDRIQALSEGRSWRDPPVVKENLGSKGRPPMGQSRRSNDSGWDDWDREGGSGGDVRSKSTGDFRNLNGGGAPARSRSTEDIYSRSQLEASAAGKEGFFAKKMAENESRPEGLPPSQGGKYVGFGSSPMPSQRSNPQQNDYFSVVSQGIGKLSLVAASAANAVQTGTKEITSKVKEGGYDQKVNETVTVVTQKTSEIGQRTWGLMKGVMALASQKVEEYTKENSNSTTNNWQRNENDRNGYYQDFNQGNKGGNSSTGREQSSSGQYKTQSSNSWDDWGQKDSWKGEPTNKGSTPYSSGGQSGTHNPSSWDDWDHKDSRKEEPAKASAPHNNDAGWAGWDNAKDDGFDNFYEGSSNKKAAGHNGKSDDAWTGGGFL