AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052122

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052122.t1 MTR_2g096760 98.194 443 8 0 1 443 1 443 0.0 905
MS.gene052122.t1 MTR_4g063830 74.273 447 109 5 1 443 1 445 0.0 684
MS.gene052122.t1 MTR_4g021880 62.207 426 148 8 18 438 104 521 0.0 526
MS.gene052122.t1 MTR_4g021880 64.935 308 103 3 18 324 104 407 3.40e-141 410
MS.gene052122.t1 MTR_0020s0130 30.752 452 233 12 1 403 1 421 2.93e-45 164
MS.gene052122.t1 MTR_4g129470 32.010 403 214 12 1 376 7 376 2.22e-41 154
MS.gene052122.t1 MTR_1g100797 31.204 407 218 11 1 376 1 376 2.52e-41 154
MS.gene052122.t1 MTR_4g129470 31.562 320 160 11 84 376 1 288 3.34e-26 110
MS.gene052122.t1 MTR_1g100797 30.769 299 146 10 109 376 4 272 3.23e-25 107
MS.gene052122.t1 MTR_1g115575 31.220 205 122 4 14 215 134 322 1.37e-18 88.6
MS.gene052122.t1 MTR_7g117620 29.661 236 125 8 15 219 85 310 2.86e-17 84.3
MS.gene052122.t1 MTR_7g100390 31.429 245 131 9 5 219 41 278 3.78e-17 84.0
MS.gene052122.t1 MTR_2g033050 31.579 171 101 5 14 179 201 360 1.17e-15 79.0
MS.gene052122.t1 MTR_2g096190 29.796 245 133 9 5 218 58 294 6.01e-15 77.4
MS.gene052122.t1 MTR_1g089180 33.152 184 109 6 45 219 252 430 7.86e-15 76.6
MS.gene052122.t1 MTR_7g115280 27.509 269 162 6 18 259 21 283 6.39e-14 73.9
MS.gene052122.t1 MTR_7g100430 30.332 211 125 5 11 215 120 314 7.26e-14 73.9
MS.gene052122.t1 MTR_4g062100 28.095 210 126 4 12 215 71 261 1.79e-13 72.8
MS.gene052122.t1 MTR_1g032100 26.471 238 142 9 11 232 53 273 2.67e-13 72.0
MS.gene052122.t1 MTR_6g027840 25.974 231 140 4 25 240 141 355 3.91e-12 68.6
MS.gene052122.t1 MTR_6g027840 29.167 216 140 4 47 253 244 455 6.59e-12 67.8
MS.gene052122.t1 MTR_5g085830 33.742 163 98 6 18 177 282 437 7.04e-12 68.2
MS.gene052122.t1 MTR_5g023090 29.240 171 104 3 45 215 292 445 1.40e-11 66.6
MS.gene052122.t1 MTR_1g112830 27.074 229 125 9 50 247 139 356 2.30e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052122.t1 AT2G28840 67.483 449 133 4 1 438 1 447 0.0 623
MS.gene052122.t1 AT2G28840 70.976 379 97 4 71 438 57 433 0.0 556
MS.gene052122.t1 AT5G07270 31.658 398 215 10 1 373 1 366 2.73e-41 154
MS.gene052122.t1 AT5G57740 31.188 404 213 10 1 373 7 376 4.71e-39 147
MS.gene052122.t1 AT5G02620 29.021 286 164 13 12 277 57 323 2.47e-19 90.9
MS.gene052122.t1 AT5G02620 29.021 286 164 13 12 277 57 323 2.47e-19 90.9
MS.gene052122.t1 AT5G02620 29.021 286 164 13 12 277 57 323 2.47e-19 90.9
MS.gene052122.t1 AT3G04710 31.538 260 145 8 22 254 25 278 1.64e-18 87.8
MS.gene052122.t1 AT3G04710 30.333 300 165 11 22 294 249 531 1.91e-18 88.6
MS.gene052122.t1 AT5G02620 30.097 206 119 8 12 211 57 243 1.96e-17 85.1
MS.gene052122.t1 AT5G02620 30.097 206 119 8 12 211 57 243 1.96e-17 85.1
MS.gene052122.t1 AT3G04710 31.538 260 144 9 22 254 25 277 2.77e-17 84.3
MS.gene052122.t1 AT3G12360 30.556 216 132 5 14 225 120 321 4.65e-17 84.0
MS.gene052122.t1 AT3G12360 30.556 216 132 5 14 225 132 333 5.14e-17 84.0
MS.gene052122.t1 AT5G60070 29.864 221 123 6 14 206 76 292 2.30e-16 81.6
MS.gene052122.t1 AT1G07710 30.806 211 117 8 14 216 69 258 1.92e-15 79.0
MS.gene052122.t1 AT1G07710 30.806 211 117 8 14 216 69 258 1.92e-15 79.0
MS.gene052122.t1 AT2G31820 27.372 274 175 8 15 278 193 452 1.13e-13 73.6
MS.gene052122.t1 AT3G09550 29.064 203 127 5 15 215 154 341 1.82e-13 72.8
MS.gene052122.t1 AT3G09550 29.064 203 127 5 15 215 147 334 1.89e-13 72.8
MS.gene052122.t1 AT3G09550 29.064 203 127 5 15 215 147 334 1.89e-13 72.8
MS.gene052122.t1 AT5G51160 29.139 151 92 4 37 180 34 176 3.50e-11 65.5

Find 95 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACACTATGCTGCTTATTA+TGG 0.234084 2.3:-5855826 MS.gene052122:CDS
CTAGTCATGTGGCTGCTTCT+TGG 0.245814 2.3:-5855762 MS.gene052122:intron
CAGAAGAAAGAATAGCTTTA+AGG 0.264068 2.3:+5855791 None:intergenic
ACTATGTTGCTTCGACAAAA+TGG 0.280496 2.3:+5854245 None:intergenic
TTGATGTTTGATACGGTTAA+TGG 0.291932 2.3:-5855859 MS.gene052122:CDS
TCTAACAAGGCTTTGGCTTC+CGG 0.299067 2.3:+5854810 None:intergenic
TACCGCTGTCGTTCAAATTT+CGG 0.312482 2.3:+5854126 None:intergenic
CTTGTTAGAACAAGCTTTGA+TGG 0.323868 2.3:-5854796 MS.gene052122:CDS
CGTATTCAGCGTGATTCATC+TGG 0.341209 2.3:-5855306 MS.gene052122:CDS
AAGTTGGGCGTCCGAAGTTC+AGG 0.350071 2.3:-5854065 MS.gene052122:CDS
CTTCATATTGCTGCTGCTAA+TGG 0.351267 2.3:-5856328 MS.gene052122:CDS
TGTGCTTCGACCGGTAGATA+TGG 0.358247 2.3:-5855513 MS.gene052122:CDS
AATAGAATATGTACACATTC+AGG 0.364281 2.3:+5855555 None:intergenic
AAGCAACATAGTTAGACTTA+TGG 0.364923 2.3:-5854234 MS.gene052122:CDS
TAGTGTGTCGTTCGGAAAGT+TGG 0.387276 2.3:-5854081 MS.gene052122:CDS
AGCTTGTTCTAACAAGGCTT+TGG 0.389126 2.3:+5854803 None:intergenic
CATCTGCTGAGCCTCTTGTT+TGG 0.394206 2.3:-5854866 MS.gene052122:CDS
CAATTTGAAGGTTACCTTGT+TGG 0.396080 2.3:+5856419 None:intergenic
GGGTGCGACGCCATTGCATT+TGG 0.399811 2.3:-5855595 MS.gene052122:CDS
CCCTTCATCTTGCTGCTAGA+GGG 0.415274 2.3:-5855374 MS.gene052122:CDS
CGCCCCATGCCAACAATTCA+CGG 0.420392 2.3:+5855334 None:intergenic
AGTGTGTCGTTCGGAAAGTT+GGG 0.435757 2.3:-5854080 MS.gene052122:CDS
TGATTGCATCCGTGAATTGT+TGG 0.439780 2.3:-5855343 MS.gene052122:CDS
CTTCGACAAAATGGACAAAC+TGG 0.445468 2.3:+5854254 None:intergenic
AGATGAAGGGGAGTGCTTCC+AGG 0.446577 2.3:+5855387 None:intergenic
TGCATTGCAGCCAACATAAG+AGG 0.453764 2.3:+5856020 None:intergenic
CCCCTTCATCTTGCTGCTAG+AGG 0.458363 2.3:-5855375 MS.gene052122:CDS
ATTCATCAGTGAACTTAATC+CGG 0.460718 2.3:-5854829 MS.gene052122:CDS
ACAGAGTTGTGACAATTTGA+AGG 0.460832 2.3:+5856407 None:intergenic
CAGGAAGGGTTGCTGCTGAA+TGG 0.461974 2.3:-5854046 MS.gene052122:CDS
AAGGGAGAAAGATATATTGA+AGG 0.462497 2.3:-5854760 MS.gene052122:CDS
TGATGGATGCAAACAGAGAA+AGG 0.466607 2.3:-5854779 MS.gene052122:CDS
GTGCTTCGACCGGTAGATAT+GGG 0.467851 2.3:-5855512 MS.gene052122:intron
CGGTTTGTTGTAGCAACAAA+GGG 0.467861 2.3:+5854304 None:intergenic
CCCCTCTAGCAGCAAGATGA+AGG 0.471186 2.3:+5855373 None:intergenic
TTGGTTGTAGGGGATTTGTT+CGG 0.476243 2.3:-5855641 MS.gene052122:intron
GAGTTATAGAAGTAGTTGTC+GGG 0.479062 2.3:+5854279 None:intergenic
ATAGAAGTAGTTGTCGGGTT+CGG 0.479189 2.3:+5854284 None:intergenic
TGTTTCATAGCAACCATGTA+TGG 0.499705 2.3:+5854924 None:intergenic
GCTCTTGTTTGTGCTTCGAC+CGG 0.500746 2.3:-5855522 MS.gene052122:CDS
CATCCGTGAATTGTTGGCAT+GGG 0.502450 2.3:-5855337 MS.gene052122:CDS
AGGGAGAAAGATATATTGAA+GGG 0.502605 2.3:-5854759 MS.gene052122:CDS
CTGATGAATTTCAGTGGAGA+TGG 0.506025 2.3:+5854843 None:intergenic
GGAGTTATAGAAGTAGTTGT+CGG 0.511884 2.3:+5854278 None:intergenic
ATGCTGCATCTGCTTTGAGC+AGG 0.517837 2.3:-5854384 MS.gene052122:CDS
CATCAAAGCTTGTTCTAACA+AGG 0.519785 2.3:+5854797 None:intergenic
CTTATGTTGGCTGCAATGCA+TGG 0.523935 2.3:-5856017 MS.gene052122:CDS
ATGTGCATTGTGCACACATT+TGG 0.527928 2.3:+5854330 None:intergenic
CTTCATCTTGCTGCTAGAGG+GGG 0.528164 2.3:-5855372 MS.gene052122:CDS
ACAATTGAAGTTCAGAATTG+TGG 0.536648 2.3:-5854356 MS.gene052122:CDS
TGCAGAGGTAACAATGATCA+TGG 0.538975 2.3:-5856457 MS.gene052122:CDS
CCCTCTAGCAGCAAGATGAA+GGG 0.539664 2.3:+5855374 None:intergenic
CCTTCATCTTGCTGCTAGAG+GGG 0.539952 2.3:-5855373 MS.gene052122:CDS
TTCTGCTCAATCTAGTCATG+TGG 0.540502 2.3:-5855773 MS.gene052122:CDS
TGTGAATGTTAGAGATGGAA+AGG 0.540835 2.3:-5855616 MS.gene052122:CDS
ATGCAGGCGTATACCATACA+TGG 0.545210 2.3:-5854937 MS.gene052122:intron
GGATTATCTAGTGTGTCGTT+CGG 0.546546 2.3:-5854089 MS.gene052122:CDS
TCGGTTTGTTGTAGCAACAA+AGG 0.550398 2.3:+5854303 None:intergenic
CTCTTCATTGCTGTCCAACA+AGG 0.550538 2.3:-5856433 MS.gene052122:CDS
GCATCCGTGAATTGTTGGCA+TGG 0.552155 2.3:-5855338 MS.gene052122:CDS
AGCGGTAGTAGCAGCTTCAA+AGG 0.552358 2.3:-5854110 MS.gene052122:CDS
CGGTTTGTGAATGTTAGAGA+TGG 0.552849 2.3:-5855621 MS.gene052122:CDS
GATGGATGCAAACAGAGAAA+GGG 0.553678 2.3:-5854778 MS.gene052122:CDS
GCATTGCAGCCAACATAAGA+GGG 0.556151 2.3:+5856021 None:intergenic
CAGCAACCCTTCCTGAACTT+CGG 0.563206 2.3:+5854054 None:intergenic
ACAAGAGGCTCAGCAGATGT+AGG 0.576412 2.3:+5854870 None:intergenic
CTCCCCGACAACATGGGTCA+AGG 0.581042 2.3:-5856487 None:intergenic
GCATGGAAGGATTGCATGTG+TGG 0.582025 2.3:-5856000 MS.gene052122:CDS
TGTTGGCTGCAATGCATGGA+AGG 0.583065 2.3:-5856013 MS.gene052122:CDS
GTGAATGTTAGAGATGGAAA+GGG 0.588584 2.3:-5855615 MS.gene052122:CDS
AGTTCACTGATGAATTTCAG+TGG 0.597323 2.3:+5854837 None:intergenic
TCTACACAATCTGATTGTGA+TGG 0.598122 2.3:+5854714 None:intergenic
GTGGAGAAGCTACTTGAAGC+TGG 0.601028 2.3:-5855981 MS.gene052122:CDS
TGTCGAGATGCCAAATGCAA+TGG 0.601204 2.3:+5855585 None:intergenic
TTCATCTTGCTGCTAGAGGG+GGG 0.602142 2.3:-5855371 MS.gene052122:CDS
ATTTGGCATCTCGACAAAGA+CGG 0.605579 2.3:-5855578 MS.gene052122:CDS
AGTGGAGATGGCCAAACAAG+AGG 0.607832 2.3:+5854855 None:intergenic
AGATCAGTACCCATATCTAC+CGG 0.613771 2.3:+5855503 None:intergenic
AGCAGATGTAGGATTTAGCA+AGG 0.613787 2.3:+5854881 None:intergenic
TGGGCGTCCGAAGTTCAGGA+AGG 0.616206 2.3:-5854061 MS.gene052122:CDS
GTATTCAGCGTGATTCATCT+GGG 0.618936 2.3:-5855305 MS.gene052122:intron
GGGCGTCCGAAGTTCAGGAA+GGG 0.620530 2.3:-5854060 MS.gene052122:CDS
ATCCGTGAATTGTTGGCATG+GGG 0.623526 2.3:-5855336 MS.gene052122:CDS
CCTCTAGCAGCAAGATGAAG+GGG 0.627465 2.3:+5855375 None:intergenic
GTTCAAAGATAAACAAGTCG+AGG 0.631719 2.3:-5854154 MS.gene052122:CDS
CAATCTGATTGTGATGGAGA+CGG 0.634456 2.3:+5854720 None:intergenic
CGACAAAATGGACAAACTGG+TGG 0.636725 2.3:+5854257 None:intergenic
TTAGCAGCAGCAATATGAAG+AGG 0.637344 2.3:+5856331 None:intergenic
GGTCAAGGTCTTAGTTGCAG+AGG 0.642603 2.3:-5856472 MS.gene052122:CDS
GGGAGAAAGATATATTGAAG+GGG 0.658402 2.3:-5854758 MS.gene052122:CDS
TGCTGCTAATGGCCAGATCG+AGG 0.658793 2.3:-5856317 MS.gene052122:intron
CTCCGAAATTTGAACGACAG+CGG 0.698163 2.3:-5854128 MS.gene052122:CDS
GTACATATTCTATTAGACAG+TGG 0.706959 2.3:-5855546 MS.gene052122:CDS
CATTGCAGCCAACATAAGAG+GGG 0.718360 2.3:+5856022 None:intergenic
TGTAGATGACAATATCTCCG+AGG 0.741974 2.3:-5854697 MS.gene052122:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAATGTTTTTGAAT+TGG - chr2.3:5854846-5854865 MS.gene052122:CDS 10.0%
!! ATGAGTATTAAAACAAATTA+TGG + chr2.3:5855384-5855403 None:intergenic 15.0%
!!! AATTATTCTGAAAAAGAAAA+AGG + chr2.3:5854775-5854794 None:intergenic 15.0%
!!! TATAATGCAAATTTTAAACT+TGG + chr2.3:5856031-5856050 None:intergenic 15.0%
!! AAAAAACACAATGCAAAATA+AGG + chr2.3:5854401-5854420 None:intergenic 20.0%
!! AAATTCAGTTCAAATTCATT+TGG + chr2.3:5855944-5855963 None:intergenic 20.0%
!! ATAATTCATCTTCATTTATG+TGG - chr2.3:5855261-5855280 MS.gene052122:intron 20.0%
!! ATTGTAACTTCTCAAATTTA+AGG - chr2.3:5855419-5855438 MS.gene052122:intron 20.0%
!! TAAATTTGAGAAGTTACAAT+AGG + chr2.3:5855419-5855438 None:intergenic 20.0%
!! TTATCATTTGTTTCAATCAA+AGG - chr2.3:5855984-5856003 MS.gene052122:CDS 20.0%
!!! AATTCTTTTTCCTTATTTTC+TGG - chr2.3:5854214-5854233 MS.gene052122:CDS 20.0%
!!! TGTTTTAAATCGAAAAAAAC+AGG - chr2.3:5854358-5854377 MS.gene052122:CDS 20.0%
!!! TTATTTTGCATTGTGTTTTT+TGG - chr2.3:5854400-5854419 MS.gene052122:CDS 20.0%
!!! TTTTTTCGATTTAAAACATC+TGG + chr2.3:5854356-5854375 None:intergenic 20.0%
! AAACAACATGAAGAAAATCT+AGG + chr2.3:5855540-5855559 None:intergenic 25.0%
! AAATCGAAAAAAACAGGTAA+TGG - chr2.3:5854364-5854383 MS.gene052122:CDS 25.0%
! AACAACATGAAGAAAATCTA+GGG + chr2.3:5855539-5855558 None:intergenic 25.0%
! AATAGAATATGTACACATTC+AGG + chr2.3:5854954-5854973 None:intergenic 25.0%
! AATTGCAAAACACAAGAATT+TGG + chr2.3:5855872-5855891 None:intergenic 25.0%
! AGGGATAAAATAATTGATGA+GGG + chr2.3:5855520-5855539 None:intergenic 25.0%
! TAGGGATAAAATAATTGATG+AGG + chr2.3:5855521-5855540 None:intergenic 25.0%
! TGTTAATCCATAGATGAATT+TGG - chr2.3:5854587-5854606 MS.gene052122:intron 25.0%
!! ATCAAACATCTAAGTCTTAT+AGG - chr2.3:5855337-5855356 MS.gene052122:CDS 25.0%
!! ATGGATTGTAGCATTTAATT+AGG - chr2.3:5855484-5855503 MS.gene052122:intron 25.0%
!! CAATTTTCCAAATTCATCTA+TGG + chr2.3:5854597-5854616 None:intergenic 25.0%
!! TAGATTTTCTTCATGTTGTT+TGG - chr2.3:5855539-5855558 MS.gene052122:CDS 25.0%
!!! AATGTTTTTGAATTGGTTGT+AGG - chr2.3:5854853-5854872 MS.gene052122:CDS 25.0%
!!! ATGTTTTTGAATTGGTTGTA+GGG - chr2.3:5854854-5854873 MS.gene052122:CDS 25.0%
!!! TCAGAATAATTCTTTTTTCC+TGG - chr2.3:5854784-5854803 MS.gene052122:CDS 25.0%
!!! TGAGAATGCTATTTCTTTTT+TGG + chr2.3:5856062-5856081 None:intergenic 25.0%
!!! TTAGCTGTTTTAAAGTTGAA+TGG - chr2.3:5855465-5855484 MS.gene052122:intron 25.0%
AAGAAATAGCATTCTCATTG+TGG - chr2.3:5856065-5856084 MS.gene052122:intron 30.0%
AAGCAACATAGTTAGACTTA+TGG - chr2.3:5856272-5856291 MS.gene052122:intron 30.0%
AAGGGAGAAAGATATATTGA+AGG - chr2.3:5855746-5855765 MS.gene052122:intron 30.0%
ACAATTGAAGTTCAGAATTG+TGG - chr2.3:5856150-5856169 MS.gene052122:CDS 30.0%
ACTGATCTCTCTAAATTCTA+TGG - chr2.3:5855018-5855037 MS.gene052122:intron 30.0%
AGGGAGAAAGATATATTGAA+GGG - chr2.3:5855747-5855766 MS.gene052122:intron 30.0%
ATTCATCAGTGAACTTAATC+CGG - chr2.3:5855677-5855696 MS.gene052122:intron 30.0%
GAAAAAAGGGTTAATCGAAA+AGG + chr2.3:5854461-5854480 None:intergenic 30.0%
GAATTTGGAAAATTGATGTG+TGG - chr2.3:5854602-5854621 MS.gene052122:intron 30.0%
GGGATAAAATAATTGATGAG+GGG + chr2.3:5855519-5855538 None:intergenic 30.0%
GTACATATTCTATTAGACAG+TGG - chr2.3:5854960-5854979 MS.gene052122:intron 30.0%
TCTTCATTTATGTGGCTTAT+TGG - chr2.3:5855269-5855288 MS.gene052122:intron 30.0%
TGGAAACTATGAAAATCTTC+AGG - chr2.3:5854622-5854641 MS.gene052122:intron 30.0%
TGTGGAATATGATATTATGC+AGG - chr2.3:5856083-5856102 MS.gene052122:intron 30.0%
TTGATGTTTGATACGGTTAA+TGG - chr2.3:5854647-5854666 MS.gene052122:intron 30.0%
! CAGAAGAAAGAATAGCTTTA+AGG + chr2.3:5854718-5854737 None:intergenic 30.0%
! TTACACTATGCTGCTTATTA+TGG - chr2.3:5854680-5854699 MS.gene052122:intron 30.0%
!! TAGCTTTAAGGCAAGAAAAA+TGG + chr2.3:5854706-5854725 None:intergenic 30.0%
!! TCAGGTTTTGATGTTTGATA+CGG - chr2.3:5854640-5854659 MS.gene052122:intron 30.0%
!! TGTTTTTGAATTGGTTGTAG+GGG - chr2.3:5854855-5854874 MS.gene052122:CDS 30.0%
!! TTTTGTTTCAGATTCTGTCA+AGG - chr2.3:5854309-5854328 MS.gene052122:CDS 30.0%
!!! AACGATCAAAGAGAGTTTTT+TGG + chr2.3:5854150-5854169 None:intergenic 30.0%
!!! TTTTTTGGTGGAAAAGAGAA+GGG + chr2.3:5854135-5854154 None:intergenic 30.0%
AAAAAGAATTCACCTCGATC+TGG + chr2.3:5854204-5854223 None:intergenic 35.0%
ACAGAGTTGTGACAATTTGA+AGG + chr2.3:5854102-5854121 None:intergenic 35.0%
AGCAGAAATGCCAGAAAATA+AGG + chr2.3:5854227-5854246 None:intergenic 35.0%
AGTTCACTGATGAATTTCAG+TGG + chr2.3:5855672-5855691 None:intergenic 35.0%
ATAAGAGGGGTCTGAAAAAA+GGG + chr2.3:5854474-5854493 None:intergenic 35.0%
CAAAAGGAGTCAAATTACCT+CGG + chr2.3:5855829-5855848 None:intergenic 35.0%
CAATTTGAAGGTTACCTTGT+TGG + chr2.3:5854090-5854109 None:intergenic 35.0%
GAGTTATAGAAGTAGTTGTC+GGG + chr2.3:5856230-5856249 None:intergenic 35.0%
GGAGTTATAGAAGTAGTTGT+CGG + chr2.3:5856231-5856250 None:intergenic 35.0%
GGCTGAGAGATTTGATTATT+TGG - chr2.3:5856005-5856024 MS.gene052122:CDS 35.0%
GGGAGAAAGATATATTGAAG+GGG - chr2.3:5855748-5855767 MS.gene052122:intron 35.0%
GTGAATGTTAGAGATGGAAA+GGG - chr2.3:5854891-5854910 MS.gene052122:CDS 35.0%
GTTCAAAGATAAACAAGTCG+AGG - chr2.3:5856352-5856371 MS.gene052122:CDS 35.0%
TCTACACAATCTGATTGTGA+TGG + chr2.3:5855795-5855814 None:intergenic 35.0%
TGTGAATGTTAGAGATGGAA+AGG - chr2.3:5854890-5854909 MS.gene052122:CDS 35.0%
TGTTTCATAGCAACCATGTA+TGG + chr2.3:5855585-5855604 None:intergenic 35.0%
! AAGCTGGTGCTAATGTAATT+TGG - chr2.3:5854541-5854560 MS.gene052122:intron 35.0%
! ACTATGTTGCTTCGACAAAA+TGG + chr2.3:5856264-5856283 None:intergenic 35.0%
! ATTCTGTCAAGGCTTTTACA+TGG - chr2.3:5854320-5854339 MS.gene052122:CDS 35.0%
! CATCAAAGCTTGTTCTAACA+AGG + chr2.3:5855712-5855731 None:intergenic 35.0%
! CGAAAAAAACAGGTAATGGT+TGG - chr2.3:5854368-5854387 MS.gene052122:CDS 35.0%
! CTTGTTAGAACAAGCTTTGA+TGG - chr2.3:5855710-5855729 MS.gene052122:intron 35.0%
! TTGAAATTTTGCAGCTATCC+TGG - chr2.3:5855101-5855120 MS.gene052122:intron 35.0%
!! GTGGCTTATTGGTTTGTATA+TGG - chr2.3:5855280-5855299 MS.gene052122:intron 35.0%
!!! CATTGTGTTTTTTGGCATGT+TGG - chr2.3:5854408-5854427 MS.gene052122:intron 35.0%
!!! GATCAAAGAGAGTTTTTTGG+TGG + chr2.3:5854147-5854166 None:intergenic 35.0%
!!! GTTTTTTGGTGGAAAAGAGA+AGG + chr2.3:5854136-5854155 None:intergenic 35.0%
AGATCAGTACCCATATCTAC+CGG + chr2.3:5855006-5855025 None:intergenic 40.0%
AGCAGATGTAGGATTTAGCA+AGG + chr2.3:5855628-5855647 None:intergenic 40.0%
ATAGAAGTAGTTGTCGGGTT+CGG + chr2.3:5856225-5856244 None:intergenic 40.0%
ATGTGCATTGTGCACACATT+TGG + chr2.3:5856179-5856198 None:intergenic 40.0%
ATTTGGCATCTCGACAAAGA+CGG - chr2.3:5854928-5854947 MS.gene052122:CDS 40.0%
CATAAGAGGGGTCTGAAAAA+AGG + chr2.3:5854475-5854494 None:intergenic 40.0%
CGGTTTGTGAATGTTAGAGA+TGG - chr2.3:5854885-5854904 MS.gene052122:CDS 40.0%
CTGATGAATTTCAGTGGAGA+TGG + chr2.3:5855666-5855685 None:intergenic 40.0%
CTTCATATTGCTGCTGCTAA+TGG - chr2.3:5854178-5854197 MS.gene052122:CDS 40.0%
CTTCGACAAAATGGACAAAC+TGG + chr2.3:5856255-5856274 None:intergenic 40.0%
GATGGATGCAAACAGAGAAA+GGG - chr2.3:5855728-5855747 MS.gene052122:intron 40.0%
GTATTCAGCGTGATTCATCT+GGG - chr2.3:5855201-5855220 MS.gene052122:intron 40.0%
GTCAAAGTCAAAGTCAATCC+AGG + chr2.3:5854805-5854824 None:intergenic 40.0%
GTTGTTTGGTACGTTTATGC+AGG - chr2.3:5855553-5855572 MS.gene052122:CDS 40.0%
TACCGCTGTCGTTCAAATTT+CGG + chr2.3:5856383-5856402 None:intergenic 40.0%
TGATTGCATCCGTGAATTGT+TGG - chr2.3:5855163-5855182 MS.gene052122:intron 40.0%
TGCAGAGGTAACAATGATCA+TGG - chr2.3:5854049-5854068 MS.gene052122:CDS 40.0%
TGTAGATGACAATATCTCCG+AGG - chr2.3:5855809-5855828 MS.gene052122:CDS 40.0%
TTAGCAGCAGCAATATGAAG+AGG + chr2.3:5854178-5854197 None:intergenic 40.0%
TTCTGCTCAATCTAGTCATG+TGG - chr2.3:5854733-5854752 MS.gene052122:CDS 40.0%
! AGCTTGTTCTAACAAGGCTT+TGG + chr2.3:5855706-5855725 None:intergenic 40.0%
! CAATCTGATTGTGATGGAGA+CGG + chr2.3:5855789-5855808 None:intergenic 40.0%
! GGATTATCTAGTGTGTCGTT+CGG - chr2.3:5856417-5856436 MS.gene052122:CDS 40.0%
! TGATGGATGCAAACAGAGAA+AGG - chr2.3:5855727-5855746 MS.gene052122:intron 40.0%
! TTGGTTGTAGGGGATTTGTT+CGG - chr2.3:5854865-5854884 MS.gene052122:CDS 40.0%
! TTTTCAGACCCCTCTTATGT+TGG - chr2.3:5854476-5854495 MS.gene052122:intron 40.0%
!! CGGTTTGTTGTAGCAACAAA+GGG + chr2.3:5856205-5856224 None:intergenic 40.0%
!! TCGGTTTGTTGTAGCAACAA+AGG + chr2.3:5856206-5856225 None:intergenic 40.0%
ATCCGTGAATTGTTGGCATG+GGG - chr2.3:5855170-5855189 MS.gene052122:intron 45.0%
ATGCAGGCGTATACCATACA+TGG - chr2.3:5855569-5855588 MS.gene052122:CDS 45.0%
CATCCGTGAATTGTTGGCAT+GGG - chr2.3:5855169-5855188 MS.gene052122:intron 45.0%
CATTGCAGCCAACATAAGAG+GGG + chr2.3:5854487-5854506 None:intergenic 45.0%
CGACAAAATGGACAAACTGG+TGG + chr2.3:5856252-5856271 None:intergenic 45.0%
CGTATTCAGCGTGATTCATC+TGG - chr2.3:5855200-5855219 MS.gene052122:intron 45.0%
CTCCGAAATTTGAACGACAG+CGG - chr2.3:5856378-5856397 MS.gene052122:CDS 45.0%
CTCTTCATTGCTGTCCAACA+AGG - chr2.3:5854073-5854092 MS.gene052122:CDS 45.0%
GCATTGCAGCCAACATAAGA+GGG + chr2.3:5854488-5854507 None:intergenic 45.0%
TGCATTGCAGCCAACATAAG+AGG + chr2.3:5854489-5854508 None:intergenic 45.0%
TGTCGAGATGCCAAATGCAA+TGG + chr2.3:5854924-5854943 None:intergenic 45.0%
! AGTGTGTCGTTCGGAAAGTT+GGG - chr2.3:5856426-5856445 MS.gene052122:CDS 45.0%
! CTTATGTTGGCTGCAATGCA+TGG - chr2.3:5854489-5854508 MS.gene052122:intron 45.0%
! TAGTGTGTCGTTCGGAAAGT+TGG - chr2.3:5856425-5856444 MS.gene052122:CDS 45.0%
! TCTAACAAGGCTTTGGCTTC+CGG + chr2.3:5855699-5855718 None:intergenic 45.0%
ACAAGAGGCTCAGCAGATGT+AGG + chr2.3:5855639-5855658 None:intergenic 50.0%
AGCGGTAGTAGCAGCTTCAA+AGG - chr2.3:5856396-5856415 MS.gene052122:CDS 50.0%
AGTGGAGATGGCCAAACAAG+AGG + chr2.3:5855654-5855673 None:intergenic 50.0%
CAGCAACCCTTCCTGAACTT+CGG + chr2.3:5856455-5856474 None:intergenic 50.0%
CAGCGTGATTCATCTGGGTA+TGG - chr2.3:5855206-5855225 MS.gene052122:intron 50.0%
CATCTGCTGAGCCTCTTGTT+TGG - chr2.3:5855640-5855659 MS.gene052122:intron 50.0%
CCCTCTAGCAGCAAGATGAA+GGG + chr2.3:5855135-5855154 None:intergenic 50.0%
CCCTTCATCTTGCTGCTAGA+GGG - chr2.3:5855132-5855151 MS.gene052122:intron 50.0%
CCTCTAGCAGCAAGATGAAG+GGG + chr2.3:5855134-5855153 None:intergenic 50.0%
CCTTCATCTTGCTGCTAGAG+GGG - chr2.3:5855133-5855152 MS.gene052122:intron 50.0%
CTAGTCATGTGGCTGCTTCT+TGG - chr2.3:5854744-5854763 MS.gene052122:CDS 50.0%
CTTCATCTTGCTGCTAGAGG+GGG - chr2.3:5855134-5855153 MS.gene052122:intron 50.0%
GCATCCGTGAATTGTTGGCA+TGG - chr2.3:5855168-5855187 MS.gene052122:intron 50.0%
GCATGGAAGGATTGCATGTG+TGG - chr2.3:5854506-5854525 MS.gene052122:intron 50.0%
GGTCAAGGTCTTAGTTGCAG+AGG - chr2.3:5854034-5854053 MS.gene052122:CDS 50.0%
GTGCTTCGACCGGTAGATAT+GGG - chr2.3:5854994-5855013 MS.gene052122:intron 50.0%
GTGGAGAAGCTACTTGAAGC+TGG - chr2.3:5854525-5854544 MS.gene052122:intron 50.0%
TATGTGTGCACGCGGACAAA+AGG + chr2.3:5855845-5855864 None:intergenic 50.0%
TGTGCTTCGACCGGTAGATA+TGG - chr2.3:5854993-5855012 MS.gene052122:intron 50.0%
TTCATCTTGCTGCTAGAGGG+GGG - chr2.3:5855135-5855154 MS.gene052122:intron 50.0%
TTGGTGTGTATGTGTGCACG+CGG + chr2.3:5855853-5855872 None:intergenic 50.0%
! ATGCTGCATCTGCTTTGAGC+AGG - chr2.3:5856122-5856141 MS.gene052122:intron 50.0%
! GCTCTTGTTTGTGCTTCGAC+CGG - chr2.3:5854984-5855003 MS.gene052122:intron 50.0%
! TGTTGGCTGCAATGCATGGA+AGG - chr2.3:5854493-5854512 MS.gene052122:intron 50.0%
AAGTTGGGCGTCCGAAGTTC+AGG - chr2.3:5856441-5856460 MS.gene052122:CDS 55.0%
CAGGAAGGGTTGCTGCTGAA+TGG - chr2.3:5856460-5856479 MS.gene052122:CDS 55.0%
CCCCTCTAGCAGCAAGATGA+AGG + chr2.3:5855136-5855155 None:intergenic 55.0%
CCCCTTCATCTTGCTGCTAG+AGG - chr2.3:5855131-5855150 MS.gene052122:intron 55.0%
CGCCCCATGCCAACAATTCA+CGG + chr2.3:5855175-5855194 None:intergenic 55.0%
TGCTGCTAATGGCCAGATCG+AGG - chr2.3:5854189-5854208 MS.gene052122:CDS 55.0%
!! AGATGAAGGGGAGTGCTTCC+AGG + chr2.3:5855122-5855141 None:intergenic 55.0%
GGGCGTCCGAAGTTCAGGAA+GGG - chr2.3:5856446-5856465 MS.gene052122:CDS 60.0%
GGGTGCGACGCCATTGCATT+TGG - chr2.3:5854911-5854930 MS.gene052122:CDS 60.0%
TGGGCGTCCGAAGTTCAGGA+AGG - chr2.3:5856445-5856464 MS.gene052122:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 5854031 5856497 5854031 ID=MS.gene052122
chr2.3 mRNA 5854031 5856497 5854031 ID=MS.gene052122.t1;Parent=MS.gene052122
chr2.3 exon 5856318 5856497 5856318 ID=MS.gene052122.t1.exon1;Parent=MS.gene052122.t1
chr2.3 CDS 5856318 5856497 5856318 ID=cds.MS.gene052122.t1;Parent=MS.gene052122.t1
chr2.3 exon 5856149 5856208 5856149 ID=MS.gene052122.t1.exon2;Parent=MS.gene052122.t1
chr2.3 CDS 5856149 5856208 5856149 ID=cds.MS.gene052122.t1;Parent=MS.gene052122.t1
chr2.3 exon 5855974 5856045 5855974 ID=MS.gene052122.t1.exon3;Parent=MS.gene052122.t1
chr2.3 CDS 5855974 5856045 5855974 ID=cds.MS.gene052122.t1;Parent=MS.gene052122.t1
chr2.3 exon 5855763 5855884 5855763 ID=MS.gene052122.t1.exon4;Parent=MS.gene052122.t1
chr2.3 CDS 5855763 5855884 5855763 ID=cds.MS.gene052122.t1;Parent=MS.gene052122.t1
chr2.3 exon 5855513 5855653 5855513 ID=MS.gene052122.t1.exon5;Parent=MS.gene052122.t1
chr2.3 CDS 5855513 5855653 5855513 ID=cds.MS.gene052122.t1;Parent=MS.gene052122.t1
chr2.3 exon 5855306 5855413 5855306 ID=MS.gene052122.t1.exon6;Parent=MS.gene052122.t1
chr2.3 CDS 5855306 5855413 5855306 ID=cds.MS.gene052122.t1;Parent=MS.gene052122.t1
chr2.3 exon 5854698 5854953 5854698 ID=MS.gene052122.t1.exon7;Parent=MS.gene052122.t1
chr2.3 CDS 5854698 5854953 5854698 ID=cds.MS.gene052122.t1;Parent=MS.gene052122.t1
chr2.3 exon 5854031 5854423 5854031 ID=MS.gene052122.t1.exon8;Parent=MS.gene052122.t1
chr2.3 CDS 5854031 5854423 5854031 ID=cds.MS.gene052122.t1;Parent=MS.gene052122.t1
Gene Sequence

>MS.gene052122

ATGGGTCAAGGTCTTAGTTGCAGAGGTAACAATGATCATGGTCTCTTCATTGCTGTCCAACAAGGTAACCTTCAAATTGTCACAACTCTGTTACAACAAGACCCTTCTCTTTTCCACCAAAAAACTCTCTTTGATCGTTTTTCACCTCTTCATATTGCTGCTGCTAATGGCCAGATCGAGGTGAATTCTTTTTCCTTATTTTCTGGCATTTCTGCTAAGTTTAGTTTAAGAATTAATATTCTATTTTGTTTTGTTTGTAACATTTCTGTTTTGTTTTGTTTTGTTTCAGATTCTGTCAAGGCTTTTACATGGATCTGTTAATCCAGATGTTTTAAATCGAAAAAAACAGGTAATGGTTGGTTATGTTCCTTATTTTGCATTGTGTTTTTTGGCATGTTGGTTTTTGTTTTTATTGCTAAAAGTGTTACCTTTTCGATTAACCCTTTTTTCAGACCCCTCTTATGTTGGCTGCAATGCATGGAAGGATTGCATGTGTGGAGAAGCTACTTGAAGCTGGTGCTAATGTAATTTGGAAAATTTCATTTTTAGTTTCATTTGTTAATCCATAGATGAATTTGGAAAATTGATGTGTGGAAACTATGAAAATCTTCAGGTTTTGATGTTTGATACGGTTAATGGAAGAACTTGTTTACACTATGCTGCTTATTATGGCCATTTTTCTTGCCTTAAAGCTATTCTTTCTTCTGCTCAATCTAGTCATGTGGCTGCTTCTTGGTTTGTCCTTTTTCTTTTTCAGAATAATTCTTTTTTCCTGGATTGACTTTGACTTTGACTTTGATGCTATGCTGCTGTTGTTTTTTAAATGTTTTTGAATTGGTTGTAGGGGATTTGTTCGGTTTGTGAATGTTAGAGATGGAAAGGGTGCGACGCCATTGCATTTGGCATCTCGACAAAGACGGCCTGAATGTGTACATATTCTATTAGACAGTGGAGCTCTTGTTTGTGCTTCGACCGGTAGATATGGGTACTGATCTCTCTAAATTCTATGGTTAATTAAAATGACTTGAATCTTATGCATTCTTCTGTTTTGTATACTAATGAATTGTTATTTGAAATTTTGCAGCTATCCTGGAAGCACTCCCCTTCATCTTGCTGCTAGAGGGGGGTCTCTTGATTGCATCCGTGAATTGTTGGCATGGGGCGCAGATCGTATTCAGCGTGATTCATCTGGGTATGGTTTGATTTTGAGACTTGTTAACATATCTAAACATAATTCATCTTCATTTATGTGGCTTATTGGTTTGTATATGGATTATTTTTGTTTATGTGTTGAAGTTATTGTAATATCAAACATCTAAGTCTTATAGGCAAATCTTTGATATGATAATTCCATAATTTGTTTTAATACTCATATCTAGCATTTTCCTATTGTAACTTCTCAAATTTAAGGAGAAACTTACTTTATTTAACGATTTAGCTGTTTTAAAGTTGAATGGATTGTAGCATTTAATTAGGCATTTTTTTCCCCTCATCAATTATTTTATCCCTAGATTTTCTTCATGTTGTTTGGTACGTTTATGCAGGCGTATACCATACATGGTTGCTATGAAACACAGACACAGATCATGCACGTCCTTGCTAAATCCTACATCTGCTGAGCCTCTTGTTTGGCCATCTCCACTGAAATTCATCAGTGAACTTAATCCGGAAGCCAAAGCCTTGTTAGAACAAGCTTTGATGGATGCAAACAGAGAAAGGGAGAAAGATATATTGAAGGGGAAATTTTACTCTCTTCCGTCTCCATCACAATCAGATTGTGTAGATGACAATATCTCCGAGGTAATTTGACTCCTTTTGTCCGCGTGCACACATACACACCAAATTCTTGTGTTTTGCAATTGAATTAATCATAATGCTGAAATGTTGCTTATTTGTTATGTTTATACAAACCAAATGAATTTGAACTGAATTTTTGTGATATGCTATCTGAATTTATCATTTGTTTCAATCAAAGGCTGAGAGATTTGATTATTTGGCCAAGTTTAAAATTTGCATTATAAATGTGATCCAAAAAAGAAATAGCATTCTCATTGTGGAATATGATATTATGCAGGTCAGTGAGTCAGAATTATGCTGCATCTGCTTTGAGCAGGTATGCACAATTGAAGTTCAGAATTGTGGTCACCAAATGTGTGCACAATGCACATTATCCCTTTGTTGCTACAACAAACCGAACCCGACAACTACTTCTATAACTCCACCAGTTTGTCCATTTTGTCGAAGCAACATAGTTAGACTTATGGTTGTCATGAAAATTGAAAATCACGACGAAACTGATCAAGACAACGTTGATATCAATTGTTCAAAGATAAACAAGTCGAGGAAACTCCGAAATTTGAACGACAGCGGTAGTAGCAGCTTCAAAGGATTATCTAGTGTGTCGTTCGGAAAGTTGGGCGTCCGAAGTTCAGGAAGGGTTGCTGCTGAATGGATTGATAAAGAATGA
Protein sequence

>MS.gene052122.t1

MGQGLSCRGNNDHGLFIAVQQGNLQIVTTLLQQDPSLFHQKTLFDRFSPLHIAAANGQIEILSRLLHGSVNPDVLNRKKQTPLMLAAMHGRIACVEKLLEAGANVLMFDTVNGRTCLHYAAYYGHFSCLKAILSSAQSSHVAASWGFVRFVNVRDGKGATPLHLASRQRRPECVHILLDSGALVCASTGRYGYPGSTPLHLAARGGSLDCIRELLAWGADRIQRDSSGRIPYMVAMKHRHRSCTSLLNPTSAEPLVWPSPLKFISELNPEAKALLEQALMDANREREKDILKGKFYSLPSPSQSDCVDDNISEVSESELCCICFEQVCTIEVQNCGHQMCAQCTLSLCCYNKPNPTTTSITPPVCPFCRSNIVRLMVVMKIENHDETDQDNVDINCSKINKSRKLRNLNDSGSSSFKGLSSVSFGKLGVRSSGRVAAEWIDKE