AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31156

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31156.t1 MTR_2g096760 97.968 443 9 0 1 443 1 443 0.0 903
MS.gene31156.t1 MTR_4g063830 74.049 447 110 5 1 443 1 445 0.0 681
MS.gene31156.t1 MTR_4g021880 61.972 426 149 6 18 438 104 521 0.0 523
MS.gene31156.t1 MTR_4g021880 64.821 307 105 2 18 324 104 407 1.90e-140 409
MS.gene31156.t1 MTR_0020s0130 30.752 452 233 12 1 403 1 421 6.55e-45 164
MS.gene31156.t1 MTR_1g100797 31.204 407 218 11 1 376 1 376 1.92e-41 154
MS.gene31156.t1 MTR_4g129470 32.010 403 214 12 1 376 7 376 2.72e-41 154
MS.gene31156.t1 MTR_4g129470 31.562 320 160 11 84 376 1 288 3.12e-26 110
MS.gene31156.t1 MTR_1g100797 30.769 299 146 10 109 376 4 272 2.14e-25 107
MS.gene31156.t1 MTR_1g115575 31.220 205 122 4 14 215 134 322 1.36e-18 88.6
MS.gene31156.t1 MTR_7g117620 29.661 236 125 8 15 219 85 310 2.34e-17 84.7
MS.gene31156.t1 MTR_7g100390 31.429 245 131 9 5 219 41 278 3.95e-17 84.0
MS.gene31156.t1 MTR_2g033050 30.769 182 110 5 3 179 190 360 1.08e-15 79.0
MS.gene31156.t1 MTR_2g096190 29.796 245 133 9 5 218 58 294 5.35e-15 77.4
MS.gene31156.t1 MTR_1g089180 33.333 183 108 6 45 218 252 429 9.15e-15 76.6
MS.gene31156.t1 MTR_7g100430 30.332 211 125 5 11 215 120 314 7.19e-14 73.9
MS.gene31156.t1 MTR_4g062100 28.095 210 126 4 12 215 71 261 1.85e-13 72.8
MS.gene31156.t1 MTR_1g032100 25.630 238 144 8 11 232 53 273 2.89e-13 72.0
MS.gene31156.t1 MTR_7g115280 27.138 269 163 6 18 259 21 283 8.45e-13 70.5
MS.gene31156.t1 MTR_6g027840 25.974 231 140 4 25 240 141 355 3.81e-12 68.6
MS.gene31156.t1 MTR_6g027840 29.032 217 139 4 47 253 244 455 4.20e-12 68.6
MS.gene31156.t1 MTR_5g085830 33.742 163 98 6 18 177 282 437 6.12e-12 68.2
MS.gene31156.t1 MTR_5g023090 29.240 171 104 3 45 215 292 445 1.69e-11 66.2
MS.gene31156.t1 MTR_1g112830 27.074 229 125 9 50 247 139 356 4.74e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31156.t1 AT2G28840 67.483 449 133 4 1 438 1 447 0.0 623
MS.gene31156.t1 AT2G28840 70.976 379 97 4 71 438 57 433 0.0 557
MS.gene31156.t1 AT5G07270 31.658 398 215 10 1 373 1 366 2.79e-41 154
MS.gene31156.t1 AT5G57740 31.188 404 213 10 1 373 7 376 6.55e-39 147
MS.gene31156.t1 AT5G02620 29.021 286 164 13 12 277 57 323 4.06e-19 90.1
MS.gene31156.t1 AT5G02620 29.021 286 164 13 12 277 57 323 4.06e-19 90.1
MS.gene31156.t1 AT5G02620 29.021 286 164 13 12 277 57 323 4.06e-19 90.1
MS.gene31156.t1 AT3G04710 31.154 260 146 8 22 254 25 278 1.72e-17 84.7
MS.gene31156.t1 AT5G02620 30.097 206 119 8 12 211 57 243 2.19e-17 85.1
MS.gene31156.t1 AT5G02620 30.097 206 119 8 12 211 57 243 2.19e-17 85.1
MS.gene31156.t1 AT3G04710 30.000 300 166 11 22 294 249 531 2.60e-17 85.1
MS.gene31156.t1 AT3G12360 30.556 216 132 5 14 225 132 333 4.66e-17 84.0
MS.gene31156.t1 AT3G12360 30.556 216 132 5 14 225 120 321 4.69e-17 84.0
MS.gene31156.t1 AT5G60070 29.864 221 123 6 14 206 76 292 2.47e-16 81.6
MS.gene31156.t1 AT3G04710 31.154 260 145 9 22 254 25 277 3.87e-16 80.9
MS.gene31156.t1 AT1G07710 30.806 211 117 8 14 216 69 258 2.08e-15 79.0
MS.gene31156.t1 AT1G07710 30.806 211 117 8 14 216 69 258 2.08e-15 79.0
MS.gene31156.t1 AT2G31820 27.372 274 175 8 15 278 193 452 5.88e-14 74.3
MS.gene31156.t1 AT3G09550 29.064 203 127 5 15 215 154 341 2.01e-13 72.8
MS.gene31156.t1 AT3G09550 29.064 203 127 5 15 215 147 334 2.14e-13 72.8
MS.gene31156.t1 AT3G09550 29.064 203 127 5 15 215 147 334 2.14e-13 72.8
MS.gene31156.t1 AT5G20350 29.204 226 124 8 50 247 35 252 4.00e-13 72.0
MS.gene31156.t1 AT5G51160 29.139 151 92 4 37 180 34 176 3.41e-11 65.5

Find 92 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACACTATGCTGCTTATTA+TGG 0.234084 2.4:-6613005 MS.gene31156:CDS
CTAGTCATGTGGCTGCTTCT+TGG 0.245814 2.4:-6612941 MS.gene31156:intron
CAGAAGAAAGAATAGCTTTA+AGG 0.264068 2.4:+6612970 None:intergenic
ACTATGTTGCTTCGACAAAA+TGG 0.280496 2.4:+6611433 None:intergenic
TTGATGTTTGATACGGTTAA+TGG 0.291932 2.4:-6613038 MS.gene31156:CDS
TCTAACAAGGCTTTGGCTTC+CGG 0.299067 2.4:+6611998 None:intergenic
TACCGCTGTCGTTCAAATTT+CGG 0.312482 2.4:+6611314 None:intergenic
CTTGTTAGAACAAGCTTTGA+TGG 0.323868 2.4:-6611984 MS.gene31156:CDS
CGTATTCAGCGTGATTCATC+TGG 0.341209 2.4:-6612491 MS.gene31156:CDS
AAGTTGGGCGTCCGAAGTTC+AGG 0.350071 2.4:-6611253 MS.gene31156:CDS
CTTCATATTGCTGCTGCTAA+TGG 0.351267 2.4:-6613497 MS.gene31156:CDS
TGTGCTTCGACCGGTAGATA+TGG 0.358247 2.4:-6612698 MS.gene31156:CDS
AATAGAATATGTACACATTC+AGG 0.364281 2.4:+6612740 None:intergenic
AAGCAACATAGTTAGACTTA+TGG 0.364923 2.4:-6611422 MS.gene31156:CDS
TAGTGTGTCGTTCGGAAAGT+TGG 0.387276 2.4:-6611269 MS.gene31156:CDS
AGCTTGTTCTAACAAGGCTT+TGG 0.389126 2.4:+6611991 None:intergenic
CATCTGCTGAGCCTCTTGTT+TGG 0.394206 2.4:-6612054 MS.gene31156:CDS
CAATTTGAAGGTTACCTTGT+TGG 0.396080 2.4:+6613588 None:intergenic
GGGTGCGACGCCGTTACATT+TGG 0.413527 2.4:-6612780 MS.gene31156:CDS
ACAGAGTTGTTACAATTTGA+AGG 0.414832 2.4:+6613576 None:intergenic
CCCTTCATCTTGCTGCTAGA+GGG 0.415274 2.4:-6612559 MS.gene31156:CDS
TGATTGTATTCGTGAATTGT+TGG 0.428838 2.4:-6612528 MS.gene31156:CDS
AGTGTGTCGTTCGGAAAGTT+GGG 0.435757 2.4:-6611268 MS.gene31156:CDS
CTTCGACAAAATGGACAAAC+TGG 0.445468 2.4:+6611442 None:intergenic
TGCATTGCAGCCAACATAAG+AGG 0.455233 2.4:+6613199 None:intergenic
CCCCTTCATCTTGCTGCTAG+AGG 0.458363 2.4:-6612560 MS.gene31156:CDS
ATTCATCAGTGAACTTAATC+CGG 0.460718 2.4:-6612017 MS.gene31156:CDS
CAGGAAGGGTTGCTGCTGAA+TGG 0.461974 2.4:-6611234 MS.gene31156:CDS
AAGGGAGAAAGATATATTGA+AGG 0.462497 2.4:-6611948 MS.gene31156:CDS
AGATGAAGGGGAGTACTTCC+AGG 0.465349 2.4:+6612572 None:intergenic
TGATGGATGCAAACAGAGAA+AGG 0.466607 2.4:-6611967 MS.gene31156:CDS
GTGCTTCGACCGGTAGATAT+GGG 0.467851 2.4:-6612697 MS.gene31156:intron
CGGTTTGTTGTAGCAACAAA+GGG 0.467861 2.4:+6611492 None:intergenic
CCCCTCTAGCAGCAAGATGA+AGG 0.471186 2.4:+6612558 None:intergenic
TTGGTTGTAGGGGATTTGTT+CGG 0.476243 2.4:-6612826 MS.gene31156:intron
GAGTTATAGAAGTAGTTGTC+GGG 0.479062 2.4:+6611467 None:intergenic
ATAGAAGTAGTTGTCGGGTT+CGG 0.479189 2.4:+6611472 None:intergenic
TATTCGTGAATTGTTGGCAT+GGG 0.499232 2.4:-6612522 MS.gene31156:CDS
TGTTTCATAGCAACCATGTA+TGG 0.499705 2.4:+6612112 None:intergenic
AGGGAGAAAGATATATTGAA+GGG 0.502605 2.4:-6611947 MS.gene31156:CDS
CTGATGAATTTCAGTGGAGA+TGG 0.506025 2.4:+6612031 None:intergenic
GGAGTTATAGAAGTAGTTGT+CGG 0.511884 2.4:+6611466 None:intergenic
GCTCTTGTTTGTGCTTCGAC+CGG 0.514971 2.4:-6612707 MS.gene31156:CDS
ATGCTGCATCTGCTTTGAGC+AGG 0.517837 2.4:-6611572 MS.gene31156:CDS
CATCAAAGCTTGTTCTAACA+AGG 0.519785 2.4:+6611985 None:intergenic
CTTATGTTGGCTGCAATGCA+TGG 0.523935 2.4:-6613196 MS.gene31156:CDS
ATGTGCATTGTGCACACATT+TGG 0.527928 2.4:+6611518 None:intergenic
CTTCATCTTGCTGCTAGAGG+GGG 0.528164 2.4:-6612557 MS.gene31156:CDS
ACAATTGAAGTTCAGAATTG+TGG 0.536648 2.4:-6611544 MS.gene31156:CDS
TGCAGAGGTAACAATGATCA+TGG 0.538975 2.4:-6613626 MS.gene31156:CDS
CCCTCTAGCAGCAAGATGAA+GGG 0.539664 2.4:+6612559 None:intergenic
CCTTCATCTTGCTGCTAGAG+GGG 0.539952 2.4:-6612558 MS.gene31156:CDS
TTCTGCTCAATCTAGTCATG+TGG 0.540502 2.4:-6612952 MS.gene31156:CDS
TGTGAATGTTAGAGATGGAA+AGG 0.540835 2.4:-6612801 MS.gene31156:CDS
ATGCAGGCGTATACCATACA+TGG 0.545210 2.4:-6612125 MS.gene31156:intron
GGATTATCTAGTGTGTCGTT+CGG 0.546546 2.4:-6611277 MS.gene31156:CDS
TCGGTTTGTTGTAGCAACAA+AGG 0.550398 2.4:+6611491 None:intergenic
CTCTTCATTGCTGTCCAACA+AGG 0.550538 2.4:-6613602 MS.gene31156:CDS
AGCGGTAGTAGCAGCTTCAA+AGG 0.552358 2.4:-6611298 MS.gene31156:CDS
CGGTTTGTGAATGTTAGAGA+TGG 0.552849 2.4:-6612806 MS.gene31156:CDS
GATGGATGCAAACAGAGAAA+GGG 0.553678 2.4:-6611966 MS.gene31156:CDS
CTCCCCGACAACATGGGTCA+AGG 0.556059 2.4:-6613656 None:intergenic
GTTCAAAGATAAACAAGTTG+AGG 0.560709 2.4:-6611342 MS.gene31156:CDS
CAGCAACCCTTCCTGAACTT+CGG 0.563206 2.4:+6611242 None:intergenic
ACAAGAGGCTCAGCAGATGT+AGG 0.576412 2.4:+6612058 None:intergenic
GCATGGAAGGATTGCATGTG+TGG 0.582025 2.4:-6613179 MS.gene31156:CDS
TGTTGGCTGCAATGCATGGA+AGG 0.583065 2.4:-6613192 MS.gene31156:CDS
GTATTCGTGAATTGTTGGCA+TGG 0.584740 2.4:-6612523 MS.gene31156:CDS
GTGAATGTTAGAGATGGAAA+GGG 0.588584 2.4:-6612800 MS.gene31156:CDS
AGTTCACTGATGAATTTCAG+TGG 0.597323 2.4:+6612025 None:intergenic
TCTACACAATCTGATTGTGA+TGG 0.598122 2.4:+6611902 None:intergenic
GTGGAGAAGCTACTTGAAGC+TGG 0.601028 2.4:-6613160 MS.gene31156:CDS
TTCATCTTGCTGCTAGAGGG+GGG 0.602142 2.4:-6612556 MS.gene31156:CDS
ATTTGGCATCTCGACAAAGA+CGG 0.606723 2.4:-6612763 MS.gene31156:CDS
AGTGGAGATGGCCAAACAAG+AGG 0.607832 2.4:+6612043 None:intergenic
AGATCAGTACCCATATCTAC+CGG 0.613771 2.4:+6612688 None:intergenic
AGCAGATGTAGGATTTAGCA+AGG 0.613787 2.4:+6612069 None:intergenic
TGGGCGTCCGAAGTTCAGGA+AGG 0.616206 2.4:-6611249 MS.gene31156:CDS
GTATTCAGCGTGATTCATCT+GGG 0.618936 2.4:-6612490 MS.gene31156:intron
TGTCGAGATGCCAAATGTAA+CGG 0.619258 2.4:+6612770 None:intergenic
GGGCGTCCGAAGTTCAGGAA+GGG 0.620530 2.4:-6611248 MS.gene31156:CDS
CCTCTAGCAGCAAGATGAAG+GGG 0.627465 2.4:+6612560 None:intergenic
CGACAAAATGGACAAACTGG+TGG 0.636725 2.4:+6611445 None:intergenic
TTAGCAGCAGCAATATGAAG+AGG 0.637344 2.4:+6613500 None:intergenic
GGTCAAGGTCTTAGTTGCAG+AGG 0.642603 2.4:-6613641 MS.gene31156:CDS
ATTCGTGAATTGTTGGCATG+GGG 0.650106 2.4:-6612521 MS.gene31156:CDS
GGGAGAAAGATATATTGAAG+GGG 0.658402 2.4:-6611946 MS.gene31156:CDS
TGCTGCTAATGGCCAGATCG+AGG 0.658793 2.4:-6613486 MS.gene31156:intron
CTCCGAAATTTGAACGACAG+CGG 0.698163 2.4:-6611316 MS.gene31156:CDS
GTACATATTCTATTAGACAG+TGG 0.720585 2.4:-6612731 MS.gene31156:CDS
GTTGCTATGAAACACAGACA+CGG 0.736330 2.4:-6612103 MS.gene31156:CDS
TGTAGATGACAATATCTCCG+AGG 0.741974 2.4:-6611885 MS.gene31156:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAATGTTTTTGAAT+TGG - chr2.4:6612018-6612037 MS.gene31156:CDS 10.0%
!!! AATTATTCTGAAAAAGAAAA+AGG + chr2.4:6611953-6611972 None:intergenic 15.0%
!!! TATAATGCAAATTTTAAACT+TGG + chr2.4:6613200-6613219 None:intergenic 15.0%
!! AAAAAACACAATGCAAAATA+AGG + chr2.4:6611579-6611598 None:intergenic 20.0%
!! AAATTCAGTTCAAATTCATT+TGG + chr2.4:6613113-6613132 None:intergenic 20.0%
!! ATAATTCATCTTCATTTATG+TGG - chr2.4:6612433-6612452 MS.gene31156:intron 20.0%
!! ATTGAAACTTCTCAAATTTA+AGG - chr2.4:6612591-6612610 MS.gene31156:intron 20.0%
!! TTATCATTTGTTTCAATCAT+AGG - chr2.4:6613153-6613172 MS.gene31156:CDS 20.0%
!!! AATTCTTTTTCCTTATTTTC+TGG - chr2.4:6611402-6611421 MS.gene31156:CDS 20.0%
!!! TGTTTTAAATCGAAAAAAAC+AGG - chr2.4:6611536-6611555 MS.gene31156:CDS 20.0%
!!! TTATTTTGCATTGTGTTTTT+TGG - chr2.4:6611578-6611597 MS.gene31156:CDS 20.0%
!!! TTTTTTCGATTTAAAACATC+TGG + chr2.4:6611534-6611553 None:intergenic 20.0%
! AAATCGAAAAAAACAGGTAA+TGG - chr2.4:6611542-6611561 MS.gene31156:CDS 25.0%
! AATAGAATATGTACACATTC+AGG + chr2.4:6612126-6612145 None:intergenic 25.0%
! AATTGCAAAACACAAGAATT+TGG + chr2.4:6613041-6613060 None:intergenic 25.0%
! AGGGATAAAATAATTGATGA+GGG + chr2.4:6612692-6612711 None:intergenic 25.0%
! GAAAAAAGGGTTAATCAAAA+AGG + chr2.4:6611639-6611658 None:intergenic 25.0%
! TAGGGATAAAATAATTGATG+AGG + chr2.4:6612693-6612712 None:intergenic 25.0%
! TGTTAATCCATAGATGAATT+TGG - chr2.4:6611765-6611784 MS.gene31156:intron 25.0%
!! ATCAAACATCTAAGTCTTTT+AGG - chr2.4:6612509-6612528 MS.gene31156:CDS 25.0%
!! ATGGATTGTAGCATTTAATT+AGG - chr2.4:6612656-6612675 MS.gene31156:intron 25.0%
!! CAATTTTCCAAATTCATCTA+TGG + chr2.4:6611775-6611794 None:intergenic 25.0%
!!! AATGTTTTTGAATTGGTTGT+AGG - chr2.4:6612025-6612044 MS.gene31156:CDS 25.0%
!!! ATGTTTTTGAATTGGTTGTA+GGG - chr2.4:6612026-6612045 MS.gene31156:CDS 25.0%
!!! TGAGAATGCTATTTCTTTTT+TGG + chr2.4:6613231-6613250 None:intergenic 25.0%
!!! TTAGCTGTTTTAAAGTTGAA+TGG - chr2.4:6612637-6612656 MS.gene31156:intron 25.0%
AAGAAATAGCATTCTCATTG+TGG - chr2.4:6613234-6613253 MS.gene31156:intron 30.0%
AAGCAACATAGTTAGACTTA+TGG - chr2.4:6613441-6613460 MS.gene31156:intron 30.0%
AAGGGAGAAAGATATATTGA+AGG - chr2.4:6612915-6612934 MS.gene31156:intron 30.0%
ACAATTGAAGTTCAGAATTG+TGG - chr2.4:6613319-6613338 MS.gene31156:intron 30.0%
ACAGAGTTGTTACAATTTGA+AGG + chr2.4:6611290-6611309 None:intergenic 30.0%
ACCAAACAACATGAAAATCT+AGG + chr2.4:6612712-6612731 None:intergenic 30.0%
ACTGATCTCTCAATATTCTA+TGG - chr2.4:6612190-6612209 MS.gene31156:intron 30.0%
AGGGAGAAAGATATATTGAA+GGG - chr2.4:6612916-6612935 MS.gene31156:intron 30.0%
ATAAGAGGAGTCTGAAAAAA+GGG + chr2.4:6611652-6611671 None:intergenic 30.0%
ATTCATCAGTGAACTTAATC+CGG - chr2.4:6612846-6612865 MS.gene31156:intron 30.0%
CCAAACAACATGAAAATCTA+GGG + chr2.4:6612711-6612730 None:intergenic 30.0%
GAATTTGGAAAATTGATGTG+TGG - chr2.4:6611780-6611799 MS.gene31156:intron 30.0%
GGGATAAAATAATTGATGAG+GGG + chr2.4:6612691-6612710 None:intergenic 30.0%
GTACATATTCTATTAGACAG+TGG - chr2.4:6612132-6612151 MS.gene31156:intron 30.0%
GTTCAAAGATAAACAAGTTG+AGG - chr2.4:6613521-6613540 MS.gene31156:CDS 30.0%
TCTTCATTTATGTGGCTTAT+TGG - chr2.4:6612441-6612460 MS.gene31156:intron 30.0%
TGATTGTATTCGTGAATTGT+TGG - chr2.4:6612335-6612354 MS.gene31156:intron 30.0%
TGGAAACTATGAAAATCTTC+AGG - chr2.4:6611800-6611819 MS.gene31156:intron 30.0%
TGTGGAATATGATATTATGC+AGG - chr2.4:6613252-6613271 MS.gene31156:intron 30.0%
TTGATGTTTGATACGGTTAA+TGG - chr2.4:6611825-6611844 MS.gene31156:intron 30.0%
! CAGAAGAAAGAATAGCTTTA+AGG + chr2.4:6611896-6611915 None:intergenic 30.0%
! TCAGAATAATTCTTTTTCCC+TGG - chr2.4:6611962-6611981 MS.gene31156:CDS 30.0%
! TTACACTATGCTGCTTATTA+TGG - chr2.4:6611858-6611877 MS.gene31156:intron 30.0%
!! TAGCTTTAAGGCAAGAAAAA+TGG + chr2.4:6611884-6611903 None:intergenic 30.0%
!! TCAGGTTTTGATGTTTGATA+CGG - chr2.4:6611818-6611837 MS.gene31156:intron 30.0%
!! TGTTTTTGAATTGGTTGTAG+GGG - chr2.4:6612027-6612046 MS.gene31156:CDS 30.0%
!! TTTTGTTTCAGATTCTGTCA+AGG - chr2.4:6611487-6611506 MS.gene31156:CDS 30.0%
!!! AACGATCAAAGAGAGTTTTT+TGG + chr2.4:6611338-6611357 None:intergenic 30.0%
!!! TTTTTTGGTGGAAAAGAGAA+GGG + chr2.4:6611323-6611342 None:intergenic 30.0%
AAAAAGAATTCACCTCGATC+TGG + chr2.4:6611392-6611411 None:intergenic 35.0%
AGCAGAAATGCCAGAAAATA+AGG + chr2.4:6611415-6611434 None:intergenic 35.0%
AGTTCACTGATGAATTTCAG+TGG + chr2.4:6612841-6612860 None:intergenic 35.0%
ATCAAAGTCAAAGTCAATCC+AGG + chr2.4:6611983-6612002 None:intergenic 35.0%
CAAAAGGAGTCAAATTACCT+CGG + chr2.4:6612998-6613017 None:intergenic 35.0%
CAATTTGAAGGTTACCTTGT+TGG + chr2.4:6611278-6611297 None:intergenic 35.0%
CATAAGAGGAGTCTGAAAAA+AGG + chr2.4:6611653-6611672 None:intergenic 35.0%
GAGTTATAGAAGTAGTTGTC+GGG + chr2.4:6613399-6613418 None:intergenic 35.0%
GGAGTTATAGAAGTAGTTGT+CGG + chr2.4:6613400-6613419 None:intergenic 35.0%
GGCTGAGAGATTTGATTATT+TGG - chr2.4:6613174-6613193 MS.gene31156:CDS 35.0%
GGGAGAAAGATATATTGAAG+GGG - chr2.4:6612917-6612936 MS.gene31156:intron 35.0%
GTGAATGTTAGAGATGGAAA+GGG - chr2.4:6612063-6612082 MS.gene31156:CDS 35.0%
TATTCGTGAATTGTTGGCAT+GGG - chr2.4:6612341-6612360 MS.gene31156:intron 35.0%
TCAAAGTCAAAGTCAATCCA+GGG + chr2.4:6611982-6612001 None:intergenic 35.0%
TCTACACAATCTGATTGTGA+TGG + chr2.4:6612964-6612983 None:intergenic 35.0%
TGTGAATGTTAGAGATGGAA+AGG - chr2.4:6612062-6612081 MS.gene31156:CDS 35.0%
TGTTTCATAGCAACCATGTA+TGG + chr2.4:6612754-6612773 None:intergenic 35.0%
! AAGCTGGTGCTAATGTAATT+TGG - chr2.4:6611719-6611738 MS.gene31156:intron 35.0%
! ACTATGTTGCTTCGACAAAA+TGG + chr2.4:6613433-6613452 None:intergenic 35.0%
! ATTCTGTCAAGGCTTTTACA+TGG - chr2.4:6611498-6611517 MS.gene31156:CDS 35.0%
! CATCAAAGCTTGTTCTAACA+AGG + chr2.4:6612881-6612900 None:intergenic 35.0%
! CCCTAGATTTTCATGTTGTT+TGG - chr2.4:6612708-6612727 MS.gene31156:CDS 35.0%
! CGAAAAAAACAGGTAATGGT+TGG - chr2.4:6611546-6611565 MS.gene31156:CDS 35.0%
! CTTGTTAGAACAAGCTTTGA+TGG - chr2.4:6612879-6612898 MS.gene31156:intron 35.0%
! TTGAAATTTTGCAGCTATCC+TGG - chr2.4:6612273-6612292 MS.gene31156:intron 35.0%
! TTTTCAGACTCCTCTTATGT+TGG - chr2.4:6611654-6611673 MS.gene31156:intron 35.0%
!! GTGGCTTATTGGTTTGTATA+TGG - chr2.4:6612452-6612471 MS.gene31156:intron 35.0%
!!! CATTGTGTTTTTTGGCATGT+TGG - chr2.4:6611586-6611605 MS.gene31156:CDS 35.0%
!!! GATCAAAGAGAGTTTTTTGG+TGG + chr2.4:6611335-6611354 None:intergenic 35.0%
!!! GTTTTTTGGTGGAAAAGAGA+AGG + chr2.4:6611324-6611343 None:intergenic 35.0%
AGATCAGTACCCATATCTAC+CGG + chr2.4:6612178-6612197 None:intergenic 40.0%
AGCAGATGTAGGATTTAGCA+AGG + chr2.4:6612797-6612816 None:intergenic 40.0%
ATAGAAGTAGTTGTCGGGTT+CGG + chr2.4:6613394-6613413 None:intergenic 40.0%
ATGTGCATTGTGCACACATT+TGG + chr2.4:6613348-6613367 None:intergenic 40.0%
ATTCGTGAATTGTTGGCATG+GGG - chr2.4:6612342-6612361 MS.gene31156:intron 40.0%
ATTTGGCATCTCGACAAAGA+CGG - chr2.4:6612100-6612119 MS.gene31156:CDS 40.0%
CGGTTTGTGAATGTTAGAGA+TGG - chr2.4:6612057-6612076 MS.gene31156:CDS 40.0%
CTGATGAATTTCAGTGGAGA+TGG + chr2.4:6612835-6612854 None:intergenic 40.0%
CTTCATATTGCTGCTGCTAA+TGG - chr2.4:6611366-6611385 MS.gene31156:CDS 40.0%
CTTCGACAAAATGGACAAAC+TGG + chr2.4:6613424-6613443 None:intergenic 40.0%
GATGGATGCAAACAGAGAAA+GGG - chr2.4:6612897-6612916 MS.gene31156:intron 40.0%
GTATTCAGCGTGATTCATCT+GGG - chr2.4:6612373-6612392 MS.gene31156:intron 40.0%
GTATTCGTGAATTGTTGGCA+TGG - chr2.4:6612340-6612359 MS.gene31156:intron 40.0%
GTTGCTATGAAACACAGACA+CGG - chr2.4:6612760-6612779 MS.gene31156:CDS 40.0%
TACCGCTGTCGTTCAAATTT+CGG + chr2.4:6613552-6613571 None:intergenic 40.0%
TGCAGAGGTAACAATGATCA+TGG - chr2.4:6611237-6611256 MS.gene31156:CDS 40.0%
TGTAGATGACAATATCTCCG+AGG - chr2.4:6612978-6612997 MS.gene31156:CDS 40.0%
TGTCGAGATGCCAAATGTAA+CGG + chr2.4:6612096-6612115 None:intergenic 40.0%
TTAGCAGCAGCAATATGAAG+AGG + chr2.4:6611366-6611385 None:intergenic 40.0%
TTCTGCTCAATCTAGTCATG+TGG - chr2.4:6611911-6611930 MS.gene31156:CDS 40.0%
! AGCTTGTTCTAACAAGGCTT+TGG + chr2.4:6612875-6612894 None:intergenic 40.0%
! GGATTATCTAGTGTGTCGTT+CGG - chr2.4:6613586-6613605 MS.gene31156:CDS 40.0%
! TGATGGATGCAAACAGAGAA+AGG - chr2.4:6612896-6612915 MS.gene31156:intron 40.0%
! TTGGTTGTAGGGGATTTGTT+CGG - chr2.4:6612037-6612056 MS.gene31156:CDS 40.0%
!! CGGTTTGTTGTAGCAACAAA+GGG + chr2.4:6613374-6613393 None:intergenic 40.0%
!! TCGGTTTGTTGTAGCAACAA+AGG + chr2.4:6613375-6613394 None:intergenic 40.0%
ATGCAGGCGTATACCATACA+TGG - chr2.4:6612738-6612757 MS.gene31156:CDS 45.0%
CGACAAAATGGACAAACTGG+TGG + chr2.4:6613421-6613440 None:intergenic 45.0%
CGTATTCAGCGTGATTCATC+TGG - chr2.4:6612372-6612391 MS.gene31156:intron 45.0%
CTCCGAAATTTGAACGACAG+CGG - chr2.4:6613547-6613566 MS.gene31156:CDS 45.0%
CTCTTCATTGCTGTCCAACA+AGG - chr2.4:6611261-6611280 MS.gene31156:CDS 45.0%
TGCATTGCAGCCAACATAAG+AGG + chr2.4:6611667-6611686 None:intergenic 45.0%
! AGTGTGTCGTTCGGAAAGTT+GGG - chr2.4:6613595-6613614 MS.gene31156:CDS 45.0%
! CTTATGTTGGCTGCAATGCA+TGG - chr2.4:6611667-6611686 MS.gene31156:intron 45.0%
! TAGTGTGTCGTTCGGAAAGT+TGG - chr2.4:6613594-6613613 MS.gene31156:CDS 45.0%
! TCTAACAAGGCTTTGGCTTC+CGG + chr2.4:6612868-6612887 None:intergenic 45.0%
!! GTTGTTTGGTGCGTTTATGC+AGG - chr2.4:6612722-6612741 MS.gene31156:CDS 45.0%
ACAAGAGGCTCAGCAGATGT+AGG + chr2.4:6612808-6612827 None:intergenic 50.0%
AGCGGTAGTAGCAGCTTCAA+AGG - chr2.4:6613565-6613584 MS.gene31156:CDS 50.0%
AGTGGAGATGGCCAAACAAG+AGG + chr2.4:6612823-6612842 None:intergenic 50.0%
CAGCAACCCTTCCTGAACTT+CGG + chr2.4:6613624-6613643 None:intergenic 50.0%
CAGCGTGATTCATCTGGGTA+TGG - chr2.4:6612378-6612397 MS.gene31156:intron 50.0%
CATCTGCTGAGCCTCTTGTT+TGG - chr2.4:6612809-6612828 MS.gene31156:CDS 50.0%
CCCTCTAGCAGCAAGATGAA+GGG + chr2.4:6612307-6612326 None:intergenic 50.0%
CCCTTCATCTTGCTGCTAGA+GGG - chr2.4:6612304-6612323 MS.gene31156:intron 50.0%
CCTCTAGCAGCAAGATGAAG+GGG + chr2.4:6612306-6612325 None:intergenic 50.0%
CCTTCATCTTGCTGCTAGAG+GGG - chr2.4:6612305-6612324 MS.gene31156:intron 50.0%
CTAGTCATGTGGCTGCTTCT+TGG - chr2.4:6611922-6611941 MS.gene31156:CDS 50.0%
CTTCATCTTGCTGCTAGAGG+GGG - chr2.4:6612306-6612325 MS.gene31156:intron 50.0%
GCATGGAAGGATTGCATGTG+TGG - chr2.4:6611684-6611703 MS.gene31156:intron 50.0%
GGTCAAGGTCTTAGTTGCAG+AGG - chr2.4:6611222-6611241 MS.gene31156:CDS 50.0%
GTGCTTCGACCGGTAGATAT+GGG - chr2.4:6612166-6612185 MS.gene31156:intron 50.0%
GTGGAGAAGCTACTTGAAGC+TGG - chr2.4:6611703-6611722 MS.gene31156:intron 50.0%
TATGTGTGCACGCGGACAAA+AGG + chr2.4:6613014-6613033 None:intergenic 50.0%
TGTGCTTCGACCGGTAGATA+TGG - chr2.4:6612165-6612184 MS.gene31156:intron 50.0%
TTCATCTTGCTGCTAGAGGG+GGG - chr2.4:6612307-6612326 MS.gene31156:intron 50.0%
TTGGTGTGTATGTGTGCACG+CGG + chr2.4:6613022-6613041 None:intergenic 50.0%
! ATGCTGCATCTGCTTTGAGC+AGG - chr2.4:6613291-6613310 MS.gene31156:intron 50.0%
! GCTCTTGTTTGTGCTTCGAC+CGG - chr2.4:6612156-6612175 MS.gene31156:intron 50.0%
! TGTTGGCTGCAATGCATGGA+AGG - chr2.4:6611671-6611690 MS.gene31156:intron 50.0%
!! AGATGAAGGGGAGTACTTCC+AGG + chr2.4:6612294-6612313 None:intergenic 50.0%
AAGTTGGGCGTCCGAAGTTC+AGG - chr2.4:6613610-6613629 MS.gene31156:CDS 55.0%
CAGGAAGGGTTGCTGCTGAA+TGG - chr2.4:6613629-6613648 MS.gene31156:CDS 55.0%
CCCCTCTAGCAGCAAGATGA+AGG + chr2.4:6612308-6612327 None:intergenic 55.0%
CCCCTTCATCTTGCTGCTAG+AGG - chr2.4:6612303-6612322 MS.gene31156:intron 55.0%
TGCTGCTAATGGCCAGATCG+AGG - chr2.4:6611377-6611396 MS.gene31156:CDS 55.0%
GGGCGTCCGAAGTTCAGGAA+GGG - chr2.4:6613615-6613634 MS.gene31156:CDS 60.0%
GGGTGCGACGCCGTTACATT+TGG - chr2.4:6612083-6612102 MS.gene31156:CDS 60.0%
TGGGCGTCCGAAGTTCAGGA+AGG - chr2.4:6613614-6613633 MS.gene31156:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6611219 6613666 6611219 ID=MS.gene31156
chr2.4 mRNA 6611219 6613666 6611219 ID=MS.gene31156.t1;Parent=MS.gene31156
chr2.4 exon 6613487 6613666 6613487 ID=MS.gene31156.t1.exon1;Parent=MS.gene31156.t1
chr2.4 CDS 6613487 6613666 6613487 ID=cds.MS.gene31156.t1;Parent=MS.gene31156.t1
chr2.4 exon 6613328 6613387 6613328 ID=MS.gene31156.t1.exon2;Parent=MS.gene31156.t1
chr2.4 CDS 6613328 6613387 6613328 ID=cds.MS.gene31156.t1;Parent=MS.gene31156.t1
chr2.4 exon 6613153 6613224 6613153 ID=MS.gene31156.t1.exon3;Parent=MS.gene31156.t1
chr2.4 CDS 6613153 6613224 6613153 ID=cds.MS.gene31156.t1;Parent=MS.gene31156.t1
chr2.4 exon 6612942 6613063 6612942 ID=MS.gene31156.t1.exon4;Parent=MS.gene31156.t1
chr2.4 CDS 6612942 6613063 6612942 ID=cds.MS.gene31156.t1;Parent=MS.gene31156.t1
chr2.4 exon 6612698 6612838 6612698 ID=MS.gene31156.t1.exon5;Parent=MS.gene31156.t1
chr2.4 CDS 6612698 6612838 6612698 ID=cds.MS.gene31156.t1;Parent=MS.gene31156.t1
chr2.4 exon 6612491 6612598 6612491 ID=MS.gene31156.t1.exon6;Parent=MS.gene31156.t1
chr2.4 CDS 6612491 6612598 6612491 ID=cds.MS.gene31156.t1;Parent=MS.gene31156.t1
chr2.4 exon 6611886 6612141 6611886 ID=MS.gene31156.t1.exon7;Parent=MS.gene31156.t1
chr2.4 CDS 6611886 6612141 6611886 ID=cds.MS.gene31156.t1;Parent=MS.gene31156.t1
chr2.4 exon 6611219 6611611 6611219 ID=MS.gene31156.t1.exon8;Parent=MS.gene31156.t1
chr2.4 CDS 6611219 6611611 6611219 ID=cds.MS.gene31156.t1;Parent=MS.gene31156.t1
Gene Sequence

>MS.gene31156

ATGGGTCAAGGTCTTAGTTGCAGAGGTAACAATGATCATGGTCTCTTCATTGCTGTCCAACAAGGTAACCTTCAAATTGTAACAACTCTGTTACAACAAGACCCTTCTCTTTTCCACCAAAAAACTCTCTTTGATCGTTTTTCACCTCTTCATATTGCTGCTGCTAATGGCCAGATCGAGGTGAATTCTTTTTCCTTATTTTCTGGCATTTCTGCTAAGTTTAGTTTAAGAATTAATATTCTATTTTGTTTTGTTTGTAACATTTCTGTTTTGTTTCAGATTCTGTCAAGGCTTTTACATGGATCTGTTAATCCAGATGTTTTAAATCGAAAAAAACAGGTAATGGTTGGTTATGTTCCTTATTTTGCATTGTGTTTTTTGGCATGTTGGTTTTTGTTTTTATTGCTATAAGTGTTACCTTTTTGATTAACCCTTTTTTCAGACTCCTCTTATGTTGGCTGCAATGCATGGAAGGATTGCATGTGTGGAGAAGCTACTTGAAGCTGGTGCTAATGTAATTTGGAAAATTTCATTTTTAGTTTCATTTGTTAATCCATAGATGAATTTGGAAAATTGATGTGTGGAAACTATGAAAATCTTCAGGTTTTGATGTTTGATACGGTTAATGGAAGAACTTGTTTACACTATGCTGCTTATTATGGCCATTTTTCTTGCCTTAAAGCTATTCTTTCTTCTGCTCAATCTAGTCATGTGGCTGCTTCTTGGTTTGTCCTTTTTCTTTTTCAGAATAATTCTTTTTCCCTGGATTGACTTTGACTTTGATGCTATGCTGTTGTTGTTTTTTAAATGTTTTTGAATTGGTTGTAGGGGATTTGTTCGGTTTGTGAATGTTAGAGATGGAAAGGGTGCGACGCCGTTACATTTGGCATCTCGACAAAGACGGCCTGAATGTGTACATATTCTATTAGACAGTGGTGCTCTTGTTTGTGCTTCGACCGGTAGATATGGGTACTGATCTCTCAATATTCTATGGTTAATTAAAATGACTTGAATCTTATGCATTCTTCTGTTTTGTGTACTAATGAATTGTTATTTGAAATTTTGCAGCTATCCTGGAAGTACTCCCCTTCATCTTGCTGCTAGAGGGGGGTCTCTTGATTGTATTCGTGAATTGTTGGCATGGGGCGCAGATCGTATTCAGCGTGATTCATCTGGGTATGGTTTGATTTTGAGACTTGTTAACATATCTAAACATAATTCATCTTCATTTATGTGGCTTATTGGTTTGTATATGGATTATTTATGTTAATGTGTTGAAGTTATTGTAATATCAAACATCTAAGTCTTTTAGGCAAATCTTTGATATGATAATTTCATAATTTGTTTTAATACTCATATCTAGCATTTTCTTATTGAAACTTCTCAAATTTAAGGAGAAACTTTCTTTATTTAACGATTTAGCTGTTTTAAAGTTGAATGGATTGTAGCATTTAATTAGGCATTTTTTTCCCCTCATCAATTATTTTATCCCTAGATTTTCATGTTGTTTGGTGCGTTTATGCAGGCGTATACCATACATGGTTGCTATGAAACACAGACACGGATCATGCACGTCCTTGCTAAATCCTACATCTGCTGAGCCTCTTGTTTGGCCATCTCCACTGAAATTCATCAGTGAACTTAATCCGGAAGCCAAAGCCTTGTTAGAACAAGCTTTGATGGATGCAAACAGAGAAAGGGAGAAAGATATATTGAAGGGGAAATTTTACTCTCTTTCGTCTCCATCACAATCAGATTGTGTAGATGACAATATCTCCGAGGTAATTTGACTCCTTTTGTCCGCGTGCACACATACACACCAAATTCTTGTGTTTTGCAATTGAATTAATCATAATGCTGAAATGTTGCTTATTTGTTATGTGTATACAAACCAAATGAATTTGAACTGAATTTTTGTGATATGCTATCTGAATTTATCATTTGTTTCAATCATAGGCTGAGAGATTTGATTATTTGGCCAAGTTTAAAATTTGCATTATAAATGTGATCCAAAAAAGAAATAGCATTCTCATTGTGGAATATGATATTATGCAGGTCAGTGAGTCAGAATTATGCTGCATCTGCTTTGAGCAGGTATGCACAATTGAAGTTCAGAATTGTGGTCACCAAATGTGTGCACAATGCACATTATCCCTTTGTTGCTACAACAAACCGAACCCGACAACTACTTCTATAACTCCACCAGTTTGTCCATTTTGTCGAAGCAACATAGTTAGACTTATGGTTGTCATGAAAATTGAAAATCACGACGAAACTGATCAAGACAACGTTGATATCAATTGTTCAAAGATAAACAAGTTGAGGAAACTCCGAAATTTGAACGACAGCGGTAGTAGCAGCTTCAAAGGATTATCTAGTGTGTCGTTCGGAAAGTTGGGCGTCCGAAGTTCAGGAAGGGTTGCTGCTGAATGGATTGATAAAGAATGA
Protein sequence

>MS.gene31156.t1

MGQGLSCRGNNDHGLFIAVQQGNLQIVTTLLQQDPSLFHQKTLFDRFSPLHIAAANGQIEILSRLLHGSVNPDVLNRKKQTPLMLAAMHGRIACVEKLLEAGANVLMFDTVNGRTCLHYAAYYGHFSCLKAILSSAQSSHVAASWGFVRFVNVRDGKGATPLHLASRQRRPECVHILLDSGALVCASTGRYGYPGSTPLHLAARGGSLDCIRELLAWGADRIQRDSSGRIPYMVAMKHRHGSCTSLLNPTSAEPLVWPSPLKFISELNPEAKALLEQALMDANREREKDILKGKFYSLSSPSQSDCVDDNISEVSESELCCICFEQVCTIEVQNCGHQMCAQCTLSLCCYNKPNPTTTSITPPVCPFCRSNIVRLMVVMKIENHDETDQDNVDINCSKINKLRKLRNLNDSGSSSFKGLSSVSFGKLGVRSSGRVAAEWIDKE