AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004045

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004045.t1 MTR_4g063830 97.927 386 8 0 1 386 62 447 0.0 785
MS.gene004045.t1 MTR_2g096760 76.425 386 86 4 1 384 61 443 0.0 612
MS.gene004045.t1 MTR_4g021880 64.398 382 128 4 1 379 147 523 4.79e-166 475
MS.gene004045.t1 MTR_4g021880 67.883 274 86 1 1 274 147 418 2.19e-121 358
MS.gene004045.t1 MTR_0020s0130 32.456 342 171 11 1 311 60 372 4.18e-35 135
MS.gene004045.t1 MTR_1g100797 30.058 346 182 11 1 315 60 376 1.26e-30 122
MS.gene004045.t1 MTR_4g129470 29.706 340 185 8 1 315 66 376 1.80e-28 116
MS.gene004045.t1 MTR_4g129470 29.968 317 168 8 24 315 1 288 4.63e-25 105
MS.gene004045.t1 MTR_1g100797 29.412 306 164 11 20 315 9 272 7.69e-23 99.4
MS.gene004045.t1 MTR_4g116160 30.811 185 110 5 5 187 89 257 2.99e-12 68.6
MS.gene004045.t1 MTR_4g116160 30.811 185 110 5 5 187 89 257 4.41e-12 67.8
MS.gene004045.t1 MTR_1g112830 29.310 174 104 4 1 171 184 341 1.70e-11 66.2
MS.gene004045.t1 MTR_1g115575 31.852 135 78 4 21 155 202 322 3.22e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004045.t1 AT2G28840 69.192 396 109 4 1 385 61 454 0.0 556
MS.gene004045.t1 AT2G28840 69.948 386 103 4 11 385 57 440 0.0 551
MS.gene004045.t1 AT5G07270 30.372 349 194 8 1 327 60 381 9.96e-33 129
MS.gene004045.t1 AT5G57740 28.402 338 187 9 1 311 66 375 1.27e-21 96.7
MS.gene004045.t1 AT3G04710 31.606 193 90 5 11 175 81 259 6.50e-12 67.4
MS.gene004045.t1 AT3G04710 31.606 193 90 5 11 175 305 483 1.07e-11 67.0
MS.gene004045.t1 AT3G04710 27.935 247 124 6 11 229 81 301 4.96e-11 64.3
MS.gene004045.t1 AT5G60070 26.894 264 159 10 21 269 144 388 8.24e-11 63.9

Find 91 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTAGAGCAGGCATTAT+TGG 0.246425 4.3:-39924236 MS.gene004045:CDS
CTAGTCCCGTGGCTGCTTCT+TGG 0.292776 4.3:-39925412 MS.gene004045:intron
AAAATGAGCGGCCGTAGTTC+CGG 0.300237 4.3:-39923075 MS.gene004045:CDS
TGTGCTTCAACTAGCAGATA+TGG 0.305279 4.3:-39925160 MS.gene004045:CDS
CGTCTCCAACGTGATGCATC+TGG 0.332475 4.3:-39924949 MS.gene004045:CDS
TGGTCATTCTTCTTGCCTTA+AGG 0.368140 4.3:-39925456 MS.gene004045:CDS
AGGCATTATTGGACGCGAAC+AGG 0.370869 4.3:-39924225 MS.gene004045:CDS
GATAACCATCAGTTTAGATA+TGG 0.374202 4.3:+39923239 None:intergenic
TGTGGAGATGGAAGAGAGTA+AGG 0.374735 4.3:+39924172 None:intergenic
AAATGAGCGGCCGTAGTTCC+GGG 0.375798 4.3:-39923074 MS.gene004045:CDS
CGAAATTTCCTTGATTTGCT+TGG 0.378282 4.3:+39923159 None:intergenic
TTGCATTATGCTGCTTACTA+TGG 0.392266 4.3:-39925476 MS.gene004045:CDS
TACTTCAAAGCAACCACATA+TGG 0.395193 4.3:+39924364 None:intergenic
AACAGAATGTGCACACATTC+AGG 0.420204 4.3:+39925202 None:intergenic
CATCTGCACAGCCTCTTGTA+TGG 0.420700 4.3:-39924306 MS.gene004045:CDS
TTGTCCAATGTAAATTCATT+CGG 0.426931 4.3:-39923099 MS.gene004045:CDS
CCTCTTCATTTAGCAGCTAA+AGG 0.427567 4.3:-39925018 MS.gene004045:CDS
CTGATTATCTTCAATGATGA+TGG 0.429013 4.3:+39924283 None:intergenic
GGGTAGCAGCAGCAGTTTCA+AGG 0.445286 4.3:-39923124 MS.gene004045:CDS
AGGTGCTACACCATTGCATC+TGG 0.451079 4.3:-39925242 MS.gene004045:CDS
TGATTGCTTCCGAAAATGAA+TGG 0.451396 4.3:-39923050 MS.gene004045:CDS
ACATCTGGTGGCCACAGTCT+TGG 0.456216 4.3:+39923355 None:intergenic
TCTAATAAAACTTTAGCCTC+TGG 0.458151 4.3:+39924250 None:intergenic
TGCTATATTTGCAGATGTCC+TGG 0.458321 4.3:-39925048 MS.gene004045:intron
ATCTAAACTGATGGTTATCA+AGG 0.465152 4.3:-39923235 MS.gene004045:CDS
GTGTGCATTGTGCACACATC+TGG 0.471356 4.3:+39923340 None:intergenic
GATATGGCGCTTCGACAGAA+TGG 0.471448 4.3:+39923255 None:intergenic
GTTTCAAGCCAAGCAAATCA+AGG 0.478084 4.3:-39923167 MS.gene004045:CDS
GTGCTTCAACTAGCAGATAT+GGG 0.478560 4.3:-39925159 MS.gene004045:intron
TATTGGACGCGAACAGGGAA+AGG 0.487813 4.3:-39924219 MS.gene004045:CDS
TGCATTGCTGCCAACATAAG+TGG 0.493653 4.3:+39925679 None:intergenic
CAGCAGAAAGTATTGCCTTA+AGG 0.496959 4.3:+39925441 None:intergenic
GGTAGCAGCAGCAGTTTCAA+GGG 0.499150 4.3:-39923123 MS.gene004045:CDS
GCTATATTTGCAGATGTCCT+GGG 0.514636 4.3:-39925047 MS.gene004045:intron
CATCCGCGAATTATTAGCAT+GGG 0.514704 4.3:-39924980 MS.gene004045:CDS
TGTGTTGAATCGACAAAAGC+AGG 0.516759 4.3:-39925841 MS.gene004045:intron
ATCTCCACACTCTGATCGAA+TGG 0.518796 4.3:-39924158 MS.gene004045:CDS
ATGCAGGCGGATACCATATG+TGG 0.519878 4.3:-39924377 MS.gene004045:intron
GTCTCCAACGTGATGCATCT+GGG 0.525701 4.3:-39924948 MS.gene004045:intron
AATGGACAGATGGGTGGGGT+GGG 0.525799 4.3:+39923273 None:intergenic
ATGCTGCATCTGCTTTGAGC+AGG 0.528277 4.3:-39923394 MS.gene004045:CDS
GACAGAATGGACAGATGGGT+GGG 0.529180 4.3:+39923268 None:intergenic
CACCCCATGCTAATAATTCG+CGG 0.532650 4.3:+39924977 None:intergenic
ATTCGATCAGAGTGTGGAGA+TGG 0.534607 4.3:+39924160 None:intergenic
GAATGGACAGATGGGTGGGG+TGG 0.536558 4.3:+39923272 None:intergenic
GGCATTATTGGACGCGAACA+GGG 0.542709 4.3:-39924224 MS.gene004045:CDS
AATTCAGACCCCACTTATGT+TGG 0.543384 4.3:-39925689 MS.gene004045:intron
CTTATGTTGGCAGCAATGCA+TGG 0.544992 4.3:-39925676 MS.gene004045:CDS
CCTTTAGCTGCTAAATGAAG+AGG 0.547668 4.3:+39925018 None:intergenic
GACTAACCAAGAAGCAGCCA+CGG 0.550497 4.3:+39925406 None:intergenic
GATTGCTTCCGAAAATGAAT+GGG 0.551989 4.3:-39923049 MS.gene004045:CDS
GCATTGCTGCCAACATAAGT+GGG 0.556318 4.3:+39925680 None:intergenic
AAGCAATCATCCCGGAACTA+CGG 0.559960 4.3:+39923064 None:intergenic
GTGAATATTAGAGACGGTAG+AGG 0.570533 4.3:-39925262 MS.gene004045:CDS
GTGGAAAAGTTGCTTGAAGC+TGG 0.573507 4.3:-39925640 MS.gene004045:CDS
ATTGGACGCGAACAGGGAAA+GGG 0.577168 4.3:-39924218 MS.gene004045:CDS
TTAATGTTTGATGTGTGTCA+TGG 0.578777 4.3:-39925509 MS.gene004045:CDS
GCTTCGACAGAATGGACAGA+TGG 0.579479 4.3:+39923263 None:intergenic
ACAGAATGGACAGATGGGTG+GGG 0.584465 4.3:+39923269 None:intergenic
GGTAGAAACTCACGAAGACA+TGG 0.587372 4.3:-39923214 MS.gene004045:CDS
CGACAGAATGGACAGATGGG+TGG 0.592181 4.3:+39923267 None:intergenic
ATCTGGCAGCTCGTCAAAGA+CGG 0.594811 4.3:-39925225 MS.gene004045:CDS
AAGCGCCATATCTAAACTGA+TGG 0.601000 4.3:-39923244 MS.gene004045:CDS
TGACGAGCTGCCAGATGCAA+TGG 0.602034 4.3:+39925232 None:intergenic
GTGCACATTCTGTTGTGTAG+TGG 0.607255 4.3:-39925193 MS.gene004045:CDS
AATGATGATGGCCATACAAG+AGG 0.608678 4.3:+39924295 None:intergenic
TGCTGCTCAATCTAGTCCCG+TGG 0.608978 4.3:-39925423 MS.gene004045:CDS
GCATGGAAAGATTGAATGTG+TGG 0.610223 4.3:-39925659 MS.gene004045:CDS
GCATCCGCGAATTATTAGCA+TGG 0.610332 4.3:-39924981 MS.gene004045:CDS
CGAATTATTAGCATGGGGTG+CGG 0.613204 4.3:-39924974 MS.gene004045:CDS
GGGATGCGAAGTTGAAGTGT+TGG 0.613230 4.3:+39923293 None:intergenic
CTTCATTTAGCAGCTAAAGG+AGG 0.613756 4.3:-39925015 MS.gene004045:CDS
AAATGAAGAGGAGTACTCCC+AGG 0.616255 4.3:+39925030 None:intergenic
CTTCGACAGAATGGACAGAT+GGG 0.616676 4.3:+39923264 None:intergenic
ACTAACCAAGAAGCAGCCAC+GGG 0.627620 4.3:+39925407 None:intergenic
AACACTCTGCTGTCACAACA+AGG 0.631175 4.3:-39923316 MS.gene004045:CDS
CGGTTTGTGAATATTAGAGA+CGG 0.645981 4.3:-39925268 MS.gene004045:CDS
TCATCCATTCGATCAGAGTG+TGG 0.646169 4.3:+39924154 None:intergenic
TGCATTGTGCACACATCTGG+TGG 0.649232 4.3:+39923343 None:intergenic
AACAGGTGTCGACATCATTG+TGG 0.652268 4.3:+39923015 None:intergenic
AATCAGTGAACTTAATCCAG+AGG 0.659408 4.3:-39924266 MS.gene004045:CDS
CACGAAGACATGGATCTAGA+CGG 0.665249 4.3:-39923204 MS.gene004045:CDS
TTTGCTTGGCTTGAAACAAG+TGG 0.668168 4.3:+39923173 None:intergenic
TGTACCCAGATGCATCACGT+TGG 0.686205 4.3:+39924944 None:intergenic
ATCCGCGAATTATTAGCATG+GGG 0.692205 4.3:-39924979 MS.gene004045:CDS
GTTGCTTTGAAGTACAAACA+TGG 0.699828 4.3:-39924355 MS.gene004045:CDS
AAATTTCGTAACATGAACGA+GGG 0.699839 4.3:-39923144 MS.gene004045:CDS
GAAATTTCGTAACATGAACG+AGG 0.700659 4.3:-39923145 MS.gene004045:CDS
CATTGCTGCCAACATAAGTG+GGG 0.709787 4.3:+39925681 None:intergenic
AATGGATGACAACATCTCTG+AGG 0.710530 4.3:-39924140 MS.gene004045:intron
ATAATTGAAGTCCAAGACTG+TGG 0.721086 4.3:-39923366 MS.gene004045:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTATGAAAAATTATC+AGG - chr4.3:39923374-39923393 MS.gene004045:CDS 10.0%
!! TATAAATTTAATAAGTTTGT+AGG + chr4.3:39924883-39924902 None:intergenic 10.0%
!!! GATTATATTTTTCAAATTAT+AGG + chr4.3:39924325-39924344 None:intergenic 10.0%
!!! TATCTTTAATTGTATTTTTT+TGG - chr4.3:39923193-39923212 MS.gene004045:CDS 10.0%
!! AAAAATATAATCACTTGTTT+CGG - chr4.3:39924333-39924352 MS.gene004045:CDS 15.0%
!! AAACTTATTAAATTTATAGC+AGG - chr4.3:39924885-39924904 MS.gene004045:intron 15.0%
!! AAATATGTAAACCTATTATT+TGG - chr4.3:39924256-39924275 MS.gene004045:CDS 15.0%
!! TCATAAAATTGAAATAAACA+TGG + chr4.3:39923318-39923337 None:intergenic 15.0%
!!! CTAATTATTTGGATATTTTT+CGG - chr4.3:39924912-39924931 MS.gene004045:intron 15.0%
!!! GATTTTAAAACATTTAACAA+TGG - chr4.3:39923585-39923604 MS.gene004045:intron 15.0%
!! AAAATTGAAATAAACATGGA+GGG + chr4.3:39923314-39923333 None:intergenic 20.0%
!! AAGAATACAATTATATTGCA+GGG - chr4.3:39923609-39923628 MS.gene004045:intron 20.0%
!! TAAAATTGAAATAAACATGG+AGG + chr4.3:39923315-39923334 None:intergenic 20.0%
!! TCATTAATTTCTTTCCTAAT+TGG - chr4.3:39923538-39923557 MS.gene004045:intron 20.0%
!! TTTAACTGATCAAAATTAGA+AGG + chr4.3:39924979-39924998 None:intergenic 20.0%
!!! GATTTGAATTTGGATTTTAA+TGG - chr4.3:39923116-39923135 MS.gene004045:CDS 20.0%
!!! TGTTTGCTATTTTTCAATTT+AGG - chr4.3:39924378-39924397 MS.gene004045:intron 20.0%
!!! TTTGGATTTTGATTTGAATT+TGG - chr4.3:39923106-39923125 MS.gene004045:CDS 20.0%
! AAGTAGTAAATTCAAATGGT+TGG + chr4.3:39924819-39924838 None:intergenic 25.0%
! AATTAATGACAAAACCGAAA+AGG + chr4.3:39923527-39923546 None:intergenic 25.0%
! ACTTGAAAGTTGAATAAAAC+AGG + chr4.3:39924471-39924490 None:intergenic 25.0%
! AGAATACAATTATATTGCAG+GGG - chr4.3:39923610-39923629 MS.gene004045:intron 25.0%
! AGCAAAGTAGTAAATTCAAA+TGG + chr4.3:39924823-39924842 None:intergenic 25.0%
! AGTTGTAGTAGTAAAATGTA+AGG - chr4.3:39924283-39924302 MS.gene004045:CDS 25.0%
! CAAGAATACAATTATATTGC+AGG - chr4.3:39923608-39923627 MS.gene004045:intron 25.0%
! GAATCATAACAATGTTTCTT+GGG + chr4.3:39924415-39924434 None:intergenic 25.0%
! GAATTTACTACTTTGCTTAT+AGG - chr4.3:39924826-39924845 MS.gene004045:intron 25.0%
! TACAACTAATGCCAAATAAT+AGG + chr4.3:39924270-39924289 None:intergenic 25.0%
! TGAATCATAACAATGTTTCT+TGG + chr4.3:39924416-39924435 None:intergenic 25.0%
! TTGTATGATCTATCAATTGT+GGG + chr4.3:39924238-39924257 None:intergenic 25.0%
! TTGTCCAATGTAAATTCATT+CGG - chr4.3:39925809-39925828 MS.gene004045:intron 25.0%
! TTTGTATGATCTATCAATTG+TGG + chr4.3:39924239-39924258 None:intergenic 25.0%
!! AAAGAGATGATAGTTTTCTA+AGG + chr4.3:39925132-39925151 None:intergenic 25.0%
!! GTTTTATCTAAGCTTTTAGA+TGG - chr4.3:39923029-39923048 MS.gene004045:CDS 25.0%
!! TTTTCCGAATGAATTTACAT+TGG + chr4.3:39925816-39925835 None:intergenic 25.0%
!!! GGTGATTTGTTAATGTTATT+TGG - chr4.3:39923088-39923107 MS.gene004045:CDS 25.0%
!!! TCCATTATACATTCACTTTT+GGG - chr4.3:39925325-39925344 MS.gene004045:intron 25.0%
!!! TGTTGAATCATTTTGTTTTG+TGG - chr4.3:39923827-39923846 MS.gene004045:intron 25.0%
AAAATGTAAGGAAGTCTCTA+AGG - chr4.3:39924295-39924314 MS.gene004045:CDS 30.0%
AAATTTCGTAACATGAACGA+GGG - chr4.3:39925764-39925783 MS.gene004045:intron 30.0%
AATTCATTCGGAAAAATGAG+CGG - chr4.3:39925821-39925840 MS.gene004045:intron 30.0%
ACTGTATCATCTCAAGTTAA+CGG + chr4.3:39924107-39924126 None:intergenic 30.0%
AGGGAGAAAAACATATTGAA+AGG - chr4.3:39924709-39924728 MS.gene004045:intron 30.0%
ATAAAACACACACAGTTTGA+AGG + chr4.3:39924513-39924532 None:intergenic 30.0%
ATATAATCACTTGTTTCGGT+AGG - chr4.3:39924337-39924356 MS.gene004045:CDS 30.0%
ATCTAAACTGATGGTTATCA+AGG - chr4.3:39925673-39925692 MS.gene004045:CDS 30.0%
CTGATTATCTTCAATGATGA+TGG + chr4.3:39924628-39924647 None:intergenic 30.0%
GAATACAATTATATTGCAGG+GGG - chr4.3:39923611-39923630 MS.gene004045:intron 30.0%
GATAACCATCAGTTTAGATA+TGG + chr4.3:39925672-39925691 None:intergenic 30.0%
TAACAATGTTTCTTGGGATA+AGG + chr4.3:39924409-39924428 None:intergenic 30.0%
TACAGCAATGCAAATGTAAA+GGG + chr4.3:39925393-39925412 None:intergenic 30.0%
TAGCAGGAAGTCTAATTATT+TGG - chr4.3:39924901-39924920 MS.gene004045:intron 30.0%
TCTAATAAAACTTTAGCCTC+TGG + chr4.3:39924661-39924680 None:intergenic 30.0%
TTAATGTTTGATGTGTGTCA+TGG - chr4.3:39923399-39923418 MS.gene004045:CDS 30.0%
TTACAGCAATGCAAATGTAA+AGG + chr4.3:39925394-39925413 None:intergenic 30.0%
TTTATTAGAGCAGGCATTAT+TGG - chr4.3:39924672-39924691 MS.gene004045:intron 30.0%
! ATCATCAACCCATTCATTTT+CGG + chr4.3:39925870-39925889 None:intergenic 30.0%
! CGAGATCTAATGTAATTTTC+AGG - chr4.3:39925475-39925494 MS.gene004045:CDS 30.0%
! CTTTCCTAATTGGTTCATAT+CGG - chr4.3:39923548-39923567 MS.gene004045:intron 30.0%
! GTCCATTATACATTCACTTT+TGG - chr4.3:39925324-39925343 MS.gene004045:intron 30.0%
!! AATGGTTGGTAAGAAATCAA+AGG + chr4.3:39924805-39924824 None:intergenic 30.0%
!! ATGGTTGGTAAGAAATCAAA+GGG + chr4.3:39924804-39924823 None:intergenic 30.0%
!! CCATTATACATTCACTTTTG+GGG - chr4.3:39925326-39925345 MS.gene004045:intron 30.0%
!! TGAAGATTTTTATTTCTCGC+TGG + chr4.3:39925421-39925440 None:intergenic 30.0%
AACTCTGAGCTATTTGTCTT+TGG - chr4.3:39925017-39925036 MS.gene004045:CDS 35.0%
AACTTGAGATTGAACTGTTG+AGG - chr4.3:39924173-39924192 MS.gene004045:CDS 35.0%
AATCAGTGAACTTAATCCAG+AGG - chr4.3:39924642-39924661 MS.gene004045:intron 35.0%
ACATAGATTACAAGTCAGAG+AGG + chr4.3:39925367-39925386 None:intergenic 35.0%
ATAATTGAAGTCCAAGACTG+TGG - chr4.3:39925542-39925561 MS.gene004045:intron 35.0%
ATCACCGATATGAACCAATT+AGG + chr4.3:39923555-39923574 None:intergenic 35.0%
ATCATCTCTTTACCATCTGT+AGG - chr4.3:39925141-39925160 MS.gene004045:intron 35.0%
ATCTCACCAATGATGATCAA+TGG - chr4.3:39924025-39924044 MS.gene004045:intron 35.0%
ATTGGTGAGATCTTATCAAG+TGG + chr4.3:39924016-39924035 None:intergenic 35.0%
CCAAATCCATTGATCATCAT+TGG + chr4.3:39924034-39924053 None:intergenic 35.0%
CCCCAAAAGTGAATGTATAA+TGG + chr4.3:39925329-39925348 None:intergenic 35.0%
CGGTTTGTGAATATTAGAGA+CGG - chr4.3:39923640-39923659 MS.gene004045:intron 35.0%
CTCGCAATAAGTATATAGTG+TGG + chr4.3:39925459-39925478 None:intergenic 35.0%
GAAATTTCGTAACATGAACG+AGG - chr4.3:39925763-39925782 MS.gene004045:intron 35.0%
GAGTTTATGCATAATTCGAC+AGG + chr4.3:39925002-39925021 None:intergenic 35.0%
GATCTATCAATTGTGGGATT+TGG + chr4.3:39924232-39924251 None:intergenic 35.0%
GATTGCTTCCGAAAATGAAT+GGG - chr4.3:39925859-39925878 MS.gene004045:CDS 35.0%
GGTGCAGTTGAATTGAATTA+TGG - chr4.3:39924847-39924866 MS.gene004045:intron 35.0%
TACTTCAAAGCAACCACATA+TGG + chr4.3:39924547-39924566 None:intergenic 35.0%
TCTAAGCCTAAAAGGTAAGT+GGG + chr4.3:39925078-39925097 None:intergenic 35.0%
TGATTGCTTCCGAAAATGAA+TGG - chr4.3:39925858-39925877 MS.gene004045:CDS 35.0%
TGGAAAGAATTGAATGTGTG+TGG - chr4.3:39923136-39923155 MS.gene004045:CDS 35.0%
TGGATTTGGATTTCCTATGA+AGG - chr4.3:39924045-39924064 MS.gene004045:intron 35.0%
! AAACAAGAGCACAATAGGAA+AGG + chr4.3:39924363-39924382 None:intergenic 35.0%
! AAACTGTGTGTGTTTTATGC+AGG - chr4.3:39924515-39924534 MS.gene004045:intron 35.0%
! ATAGCAAACAAGAGCACAAT+AGG + chr4.3:39924368-39924387 None:intergenic 35.0%
! CCAATGATGATCAATGGATT+TGG - chr4.3:39924031-39924050 MS.gene004045:intron 35.0%
! TTGCATTATGCTGCTTACTA+TGG - chr4.3:39923432-39923451 MS.gene004045:intron 35.0%
!! CGAAATTTCCTTGATTTGCT+TGG + chr4.3:39925752-39925771 None:intergenic 35.0%
!! GTTGCTTTGAAGTACAAACA+TGG - chr4.3:39924553-39924572 MS.gene004045:intron 35.0%
!!! GGCTAAAGTTTTATTAGAGC+AGG - chr4.3:39924663-39924682 MS.gene004045:intron 35.0%
!!! TAATTTTCAGGTTAGCGAGA+CGG - chr4.3:39925487-39925506 MS.gene004045:CDS 35.0%
!!! TTTTGTCGATTCAACACATC+TGG + chr4.3:39923065-39923084 None:intergenic 35.0%
AACAGAATGTGCACACATTC+AGG + chr4.3:39923709-39923728 None:intergenic 40.0%
AACGATGAGTCTGATAAGCT+TGG - chr4.3:39923792-39923811 MS.gene004045:intron 40.0%
AAGCGCCATATCTAAACTGA+TGG - chr4.3:39925664-39925683 MS.gene004045:CDS 40.0%
AATGGATGACAACATCTCTG+AGG - chr4.3:39924768-39924787 MS.gene004045:intron 40.0%
AATTCAGACCCCACTTATGT+TGG - chr4.3:39923219-39923238 MS.gene004045:CDS 40.0%
ATCCGCGAATTATTAGCATG+GGG - chr4.3:39923929-39923948 MS.gene004045:intron 40.0%
CATCCGCGAATTATTAGCAT+GGG - chr4.3:39923928-39923947 MS.gene004045:intron 40.0%
CCTCTTCATTTAGCAGCTAA+AGG - chr4.3:39923890-39923909 MS.gene004045:intron 40.0%
CCTTTAGCTGCTAAATGAAG+AGG + chr4.3:39923893-39923912 None:intergenic 40.0%
CGATCATCATCATGTCAAAC+AGG - chr4.3:39925199-39925218 MS.gene004045:CDS 40.0%
CTCTAAGCCTAAAAGGTAAG+TGG + chr4.3:39925079-39925098 None:intergenic 40.0%
CTTCATTTAGCAGCTAAAGG+AGG - chr4.3:39923893-39923912 MS.gene004045:intron 40.0%
GCATGGAAAGATTGAATGTG+TGG - chr4.3:39923249-39923268 MS.gene004045:CDS 40.0%
GCTATATTTGCAGATGTCCT+GGG - chr4.3:39923861-39923880 MS.gene004045:intron 40.0%
GGTAACTTCTAACCTACAGA+TGG + chr4.3:39925156-39925175 None:intergenic 40.0%
GTGAATATTAGAGACGGTAG+AGG - chr4.3:39923646-39923665 MS.gene004045:intron 40.0%
GTGCTTCAACTAGCAGATAT+GGG - chr4.3:39923749-39923768 MS.gene004045:intron 40.0%
GTTTCAAGCCAAGCAAATCA+AGG - chr4.3:39925741-39925760 MS.gene004045:intron 40.0%
TAGATTACAAGTCAGAGAGG+CGG + chr4.3:39925364-39925383 None:intergenic 40.0%
TGCTATATTTGCAGATGTCC+TGG - chr4.3:39923860-39923879 MS.gene004045:intron 40.0%
TGGTCATTCTTCTTGCCTTA+AGG - chr4.3:39923452-39923471 MS.gene004045:intron 40.0%
TGTGCTTCAACTAGCAGATA+TGG - chr4.3:39923748-39923767 MS.gene004045:intron 40.0%
TGTGTTGAATCGACAAAAGC+AGG - chr4.3:39923067-39923086 MS.gene004045:CDS 40.0%
! ACATGATGATGATCGCTTGA+TGG + chr4.3:39925194-39925213 None:intergenic 40.0%
! ATTTTCGGAAGCAATCATCC+CGG + chr4.3:39925855-39925874 None:intergenic 40.0%
! CATGATGATGATCGCTTGAT+GGG + chr4.3:39925193-39925212 None:intergenic 40.0%
! TACCTTTTAGGCTTAGAGCT+CGG - chr4.3:39925081-39925100 MS.gene004045:intron 40.0%
! TTTGCTTGGCTTGAAACAAG+TGG + chr4.3:39925738-39925757 None:intergenic 40.0%
!! AATGATGATGGCCATACAAG+AGG + chr4.3:39924616-39924635 None:intergenic 40.0%
!! CAGCAGAAAGTATTGCCTTA+AGG + chr4.3:39923470-39923489 None:intergenic 40.0%
!! GACAACATCTCTGAGGTAAT+TGG - chr4.3:39924775-39924794 MS.gene004045:intron 40.0%
!! GCTTCTTGGTTAGTCCTTTT+CGG - chr4.3:39923510-39923529 MS.gene004045:intron 40.0%
AACACTCTGCTGTCACAACA+AGG - chr4.3:39925592-39925611 MS.gene004045:intron 45.0%
AAGCAATCATCCCGGAACTA+CGG + chr4.3:39925847-39925866 None:intergenic 45.0%
ATCTCCACACTCTGATCGAA+TGG - chr4.3:39924750-39924769 MS.gene004045:intron 45.0%
ATTCGATCAGAGTGTGGAGA+TGG + chr4.3:39924751-39924770 None:intergenic 45.0%
CACCCCATGCTAATAATTCG+CGG + chr4.3:39923934-39923953 None:intergenic 45.0%
CACGAAGACATGGATCTAGA+CGG - chr4.3:39925704-39925723 MS.gene004045:intron 45.0%
CATTGCTGCCAACATAAGTG+GGG + chr4.3:39923230-39923249 None:intergenic 45.0%
CGAATTATTAGCATGGGGTG+CGG - chr4.3:39923934-39923953 MS.gene004045:intron 45.0%
CTTATGTTGGCAGCAATGCA+TGG - chr4.3:39923232-39923251 MS.gene004045:CDS 45.0%
CTTCGACAGAATGGACAGAT+GGG + chr4.3:39925647-39925666 None:intergenic 45.0%
GAAGTTACCAATGCGTGTAG+CGG - chr4.3:39925167-39925186 MS.gene004045:CDS 45.0%
GCATCCGCGAATTATTAGCA+TGG - chr4.3:39923927-39923946 MS.gene004045:intron 45.0%
GCATTGCTGCCAACATAAGT+GGG + chr4.3:39923231-39923250 None:intergenic 45.0%
GGTAGAAACTCACGAAGACA+TGG - chr4.3:39925694-39925713 MS.gene004045:intron 45.0%
GTGCACATTCTGTTGTGTAG+TGG - chr4.3:39923715-39923734 MS.gene004045:intron 45.0%
GTGGAAAAGTTGCTTGAAGC+TGG - chr4.3:39923268-39923287 MS.gene004045:CDS 45.0%
TATATTGCAGGGGGTTTGCA+CGG - chr4.3:39923620-39923639 MS.gene004045:intron 45.0%
TCATCCATTCGATCAGAGTG+TGG + chr4.3:39924757-39924776 None:intergenic 45.0%
TGCATTGCTGCCAACATAAG+TGG + chr4.3:39923232-39923251 None:intergenic 45.0%
TGTGGAGATGGAAGAGAGTA+AGG + chr4.3:39924739-39924758 None:intergenic 45.0%
! ACCTTTTAGGCTTAGAGCTC+GGG - chr4.3:39925082-39925101 MS.gene004045:intron 45.0%
! CTGTGTGTGTTTTATGCAGG+CGG - chr4.3:39924518-39924537 MS.gene004045:intron 45.0%
! GTCAAGCCCACTTACCTTTT+AGG - chr4.3:39925069-39925088 MS.gene004045:intron 45.0%
! TCTAAGGTAGCTCGTTGAGA+TGG + chr4.3:39925116-39925135 None:intergenic 45.0%
!! AAATGAAGAGGAGTACTCCC+AGG + chr4.3:39923881-39923900 None:intergenic 45.0%
!!! CAGCGTGCTTTTTCCTTCAT+AGG + chr4.3:39924061-39924080 None:intergenic 45.0%
AAAATGAGCGGCCGTAGTTC+CGG - chr4.3:39925833-39925852 MS.gene004045:intron 50.0%
ACAGAATGGACAGATGGGTG+GGG + chr4.3:39925642-39925661 None:intergenic 50.0%
ACTAACCAAGAAGCAGCCAC+GGG + chr4.3:39923504-39923523 None:intergenic 50.0%
AGGCATTATTGGACGCGAAC+AGG - chr4.3:39924683-39924702 MS.gene004045:intron 50.0%
ATCTGGCAGCTCGTCAAAGA+CGG - chr4.3:39923683-39923702 MS.gene004045:intron 50.0%
ATGCAGGCGGATACCATATG+TGG - chr4.3:39924531-39924550 MS.gene004045:intron 50.0%
ATTGGACGCGAACAGGGAAA+GGG - chr4.3:39924690-39924709 MS.gene004045:intron 50.0%
CATCTGCACAGCCTCTTGTA+TGG - chr4.3:39924602-39924621 MS.gene004045:intron 50.0%
GACAGAATGGACAGATGGGT+GGG + chr4.3:39925643-39925662 None:intergenic 50.0%
GACTAACCAAGAAGCAGCCA+CGG + chr4.3:39923505-39923524 None:intergenic 50.0%
GATATGGCGCTTCGACAGAA+TGG + chr4.3:39925656-39925675 None:intergenic 50.0%
GCTTCGACAGAATGGACAGA+TGG + chr4.3:39925648-39925667 None:intergenic 50.0%
GGCATTATTGGACGCGAACA+GGG - chr4.3:39924684-39924703 MS.gene004045:intron 50.0%
GGCTTGACTAAGTCGTTACC+CGG + chr4.3:39925057-39925076 None:intergenic 50.0%
GGGATGCGAAGTTGAAGTGT+TGG + chr4.3:39925618-39925637 None:intergenic 50.0%
GGTAGCAGCAGCAGTTTCAA+GGG - chr4.3:39925785-39925804 MS.gene004045:intron 50.0%
GTCTCCAACGTGATGCATCT+GGG - chr4.3:39923960-39923979 MS.gene004045:intron 50.0%
GTGTGCATTGTGCACACATC+TGG + chr4.3:39925571-39925590 None:intergenic 50.0%
TATTGGACGCGAACAGGGAA+AGG - chr4.3:39924689-39924708 MS.gene004045:intron 50.0%
TGCATTGTGCACACATCTGG+TGG + chr4.3:39925568-39925587 None:intergenic 50.0%
TGTACCCAGATGCATCACGT+TGG + chr4.3:39923967-39923986 None:intergenic 50.0%
! AGGTGCTACACCATTGCATC+TGG - chr4.3:39923666-39923685 MS.gene004045:intron 50.0%
! ATGCTGCATCTGCTTTGAGC+AGG - chr4.3:39925514-39925533 MS.gene004045:CDS 50.0%
! CCTTTTAGGCTTAGAGCTCG+GGG - chr4.3:39925083-39925102 MS.gene004045:intron 50.0%
AAATGAGCGGCCGTAGTTCC+GGG - chr4.3:39925834-39925853 MS.gene004045:intron 55.0%
AATGGACAGATGGGTGGGGT+GGG + chr4.3:39925638-39925657 None:intergenic 55.0%
ACATCTGGTGGCCACAGTCT+TGG + chr4.3:39925556-39925575 None:intergenic 55.0%
CGACAGAATGGACAGATGGG+TGG + chr4.3:39925644-39925663 None:intergenic 55.0%
CGTCTCCAACGTGATGCATC+TGG - chr4.3:39923959-39923978 MS.gene004045:intron 55.0%
GACTAAGTCGTTACCCGGCT+GGG + chr4.3:39925052-39925071 None:intergenic 55.0%
GGGTAGCAGCAGCAGTTTCA+AGG - chr4.3:39925784-39925803 MS.gene004045:intron 55.0%
TGACGAGCTGCCAGATGCAA+TGG + chr4.3:39923679-39923698 None:intergenic 55.0%
TGACTAAGTCGTTACCCGGC+TGG + chr4.3:39925053-39925072 None:intergenic 55.0%
TGCTGCTCAATCTAGTCCCG+TGG - chr4.3:39923485-39923504 MS.gene004045:intron 55.0%
!! CCCCGAGCTCTAAGCCTAAA+AGG + chr4.3:39925086-39925105 None:intergenic 55.0%
CTAGTCCCGTGGCTGCTTCT+TGG - chr4.3:39923496-39923515 MS.gene004045:intron 60.0%
GAATGGACAGATGGGTGGGG+TGG + chr4.3:39925639-39925658 None:intergenic 60.0%
! TGATGGGCCGCTACACGCAT+TGG + chr4.3:39925177-39925196 None:intergenic 60.0%
!! TTTGGCTCTGCTGTCCCAGC+CGG - chr4.3:39925035-39925054 MS.gene004045:CDS 60.0%
!! TTGGCTCTGCTGTCCCAGCC+GGG - chr4.3:39925036-39925055 MS.gene004045:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 39923029 39925901 39923029 ID=MS.gene004045
chr4.3 mRNA 39923029 39925901 39923029 ID=MS.gene004045.t1;Parent=MS.gene004045
chr4.3 exon 39925842 39925901 39925842 ID=MS.gene004045.t1.exon1;Parent=MS.gene004045.t1
chr4.3 CDS 39925842 39925901 39925842 ID=cds.MS.gene004045.t1;Parent=MS.gene004045.t1
chr4.3 exon 39925633 39925704 39925633 ID=MS.gene004045.t1.exon2;Parent=MS.gene004045.t1
chr4.3 CDS 39925633 39925704 39925633 ID=cds.MS.gene004045.t1;Parent=MS.gene004045.t1
chr4.3 exon 39925413 39925534 39925413 ID=MS.gene004045.t1.exon3;Parent=MS.gene004045.t1
chr4.3 CDS 39925413 39925534 39925413 ID=cds.MS.gene004045.t1;Parent=MS.gene004045.t1
chr4.3 exon 39925160 39925300 39925160 ID=MS.gene004045.t1.exon4;Parent=MS.gene004045.t1
chr4.3 CDS 39925160 39925300 39925160 ID=cds.MS.gene004045.t1;Parent=MS.gene004045.t1
chr4.3 exon 39924949 39925056 39924949 ID=MS.gene004045.t1.exon5;Parent=MS.gene004045.t1
chr4.3 CDS 39924949 39925056 39924949 ID=cds.MS.gene004045.t1;Parent=MS.gene004045.t1
chr4.3 exon 39924141 39924393 39924141 ID=MS.gene004045.t1.exon6;Parent=MS.gene004045.t1
chr4.3 CDS 39924141 39924393 39924141 ID=cds.MS.gene004045.t1;Parent=MS.gene004045.t1
chr4.3 exon 39923029 39923433 39923029 ID=MS.gene004045.t1.exon7;Parent=MS.gene004045.t1
chr4.3 CDS 39923029 39923433 39923029 ID=cds.MS.gene004045.t1;Parent=MS.gene004045.t1
Gene Sequence

>MS.gene004045

GTTTTATCTAAGCTTTTAGATGGATCTGCGAATCCAGATGTGTTGAATCGACAAAAGCAGGTGATTTGTTAATGTTATTTGGATTTTGATTTGAATTTGGATTTTAATGGAAAGAATTGAATGTGTGTGGAGAATCTTTTATCTTTTTGTGTTTGTTTTTTACTTATCTTTAATTGTATTTTTTTGGAACAATTCAGACCCCACTTATGTTGGCAGCAATGCATGGAAAGATTGAATGTGTGGAAAAGTTGCTTGAAGCTGGTGCTAATGTATGTTACATTTCCCTCCATGTTTATTTCAATTTTATGAATTTTATTATATTTATTTAATTATTTTATTAAAACTAATAATTATGAAAAATTATCAGGTTTTAATGTTTGATGTGTGTCATGGAAGAACTTGCTTGCATTATGCTGCTTACTATGGTCATTCTTCTTGCCTTAAGGCAATACTTTCTGCTGCTCAATCTAGTCCCGTGGCTGCTTCTTGGTTAGTCCTTTTCGGTTTTGTCATTAATTTCTTTCCTAATTGGTTCATATCGGTGATGTATTTTGATGATTTTAAAACATTTAACAATGGCAAGAATACAATTATATTGCAGGGGGTTTGCACGGTTTGTGAATATTAGAGACGGTAGAGGTGCTACACCATTGCATCTGGCAGCTCGTCAAAGACGGCCTGAATGTGTGCACATTCTGTTGTGTAGTGGAGCTCTTGTTTGTGCTTCAACTAGCAGATATGGGTAATTATTTTTCAGTGTTTTAACGATGAGTCTGATAAGCTTGGATTTGCTCTATGTGTTGAATCATTTTGTTTTGTGGATTAATATGTTGCTATATTTGCAGATGTCCTGGGAGTACTCCTCTTCATTTAGCAGCTAAAGGAGGATCTCTCGATTGCATCCGCGAATTATTAGCATGGGGTGCGGATCGTCTCCAACGTGATGCATCTGGGTACATTCTAGTTTCTCAATATGAATTCTTACCACTTGATAAGATCTCACCAATGATGATCAATGGATTTGGATTTCCTATGAAGGAAAAAGCACGCTGTTATACACAGTTTTTGATCTCAACCGTTAACTTGAGATGATACAGTTCAGATTACACAATAACAAACTTCGTTTAAAAAGATCTCGACTGTTAACTTGAGATTGAACTGTTGAGGTTGAAACTGCGTGATGTAGTTACTGCACAAAATCCAAATCCCACAATTGATAGATCATACAAATATGTAAACCTATTATTTGGCATTAGTTGTAGTAGTAAAATGTAAGGAAGTCTCTAAGGAAAACCTATAATTTGAAAAATATAATCACTTGTTTCGGTAGGCCTTTCCTATTGTGCTCTTGTTTGCTATTTTTCAATTTAGGATTCACCTTATCCCAAGAAACATTGTTATGATTCATAAGTCGAGAAATTCTTACAATATAAACTATACCTGTTTTATTCAACTTTCAAGTCTGAAAATTTCTTAAATTTCCTTCAAACTGTGTGTGTTTTATGCAGGCGGATACCATATGTGGTTGCTTTGAAGTACAAACATGGAGCATGTGCAGCATTGCTAAATCTTTCATCTGCACAGCCTCTTGTATGGCCATCATCATTGAAGATAATCAGTGAACTTAATCCAGAGGCTAAAGTTTTATTAGAGCAGGCATTATTGGACGCGAACAGGGAAAGGGAGAAAAACATATTGAAAGGCAGTCCTTACTCTCTTCCATCTCCACACTCTGATCGAATGGATGACAACATCTCTGAGGTAATTGGACACCCTTTGATTTCTTACCAACCATTTGAATTTACTACTTTGCTTATAGGTGCAGTTGAATTGAATTATGGCAATATAGTTCCTACAAACTTATTAAATTTATAGCAGGAAGTCTAATTATTTGGATATTTTTCGGAATATGCAGCAAATCAAAAGTTTATACTTTTAAGTTTTTGTCCTTCTAATTTTGATCAGTTAAACCTGTCGAATTATGCATAAACTCTGAGCTATTTGTCTTTGGCTCTGCTGTCCCAGCCGGGTAACGACTTAGTCAAGCCCACTTACCTTTTAGGCTTAGAGCTCGGGGTTGTCTGCCATCTCAACGAGCTACCTTAGAAAACTATCATCTCTTTACCATCTGTAGGTTAGAAGTTACCAATGCGTGTAGCGGCCCATCAAGCGATCATCATCATGTCAAACAGGCTCTGAACTATTGAAACTTCTCTTGAACAAATGAAACAACTCTACGATACTGATTAAAAAATGTCAGAATTTATTGTATTTCAAATTCTAGTCAAGTTTATAGTCCATTATACATTCACTTTTGGGGCAAGTTTGAAATCCGCCTCTCTGACTTGTAATCTATGTTTTCCCTTTACATTTGCATTGCTGTAACACACCAGCGAGAAATAAAAATCTTCACTTTTTTTTCTTCAACCACACTATATACTTATTGCGAGATCTAATGTAATTTTCAGGTTAGCGAGACGGAATTATGCTGCATCTGCTTTGAGCAGGTGTGTATAATTGAAGTCCAAGACTGTGGCCACCAGATGTGTGCACAATGCACACTAACACTCTGCTGTCACAACAAGGCCAACACTTCAACTTCGCATCCCACCCCACCCATCTGTCCATTCTGTCGAAGCGCCATATCTAAACTGATGGTTATCAAGGTAGAAACTCACGAAGACATGGATCTAGACGGCGCTGATACCACTTGTTTCAAGCCAAGCAAATCAAGGAAATTTCGTAACATGAACGAGGGTAGCAGCAGCAGTTTCAAGGGATTGTCCAATGTAAATTCATTCGGAAAAATGAGCGGCCGTAGTTCCGGGATGATTGCTTCCGAAAATGAATGGGTTGATGATAAGCCACAATGA
Protein sequence

>MS.gene004045.t1

VLSKLLDGSANPDVLNRQKQTPLMLAAMHGKIECVEKLLEAGANVLMFDVCHGRTCLHYAAYYGHSSCLKAILSAAQSSPVAASWGFARFVNIRDGRGATPLHLAARQRRPECVHILLCSGALVCASTSRYGCPGSTPLHLAAKGGSLDCIRELLAWGADRLQRDASGRIPYVVALKYKHGACAALLNLSSAQPLVWPSSLKIISELNPEAKVLLEQALLDANREREKNILKGSPYSLPSPHSDRMDDNISEVSETELCCICFEQVCIIEVQDCGHQMCAQCTLTLCCHNKANTSTSHPTPPICPFCRSAISKLMVIKVETHEDMDLDGADTTCFKPSKSRKFRNMNEGSSSSFKGLSNVNSFGKMSGRSSGMIASENEWVDDKPQ