AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30955

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30955.t1 MTR_2g096760 97.968 443 9 0 1 443 1 443 0.0 905
MS.gene30955.t1 MTR_4g063830 74.049 447 110 5 1 443 1 445 0.0 684
MS.gene30955.t1 MTR_4g021880 61.593 427 149 7 18 438 104 521 0.0 521
MS.gene30955.t1 MTR_4g021880 64.286 308 105 3 18 324 104 407 1.08e-139 407
MS.gene30955.t1 MTR_0020s0130 30.752 452 233 12 1 403 1 421 1.30e-44 163
MS.gene30955.t1 MTR_4g129470 32.010 403 214 12 1 376 7 376 2.83e-41 154
MS.gene30955.t1 MTR_1g100797 31.204 407 218 11 1 376 1 376 3.51e-41 153
MS.gene30955.t1 MTR_4g129470 31.562 320 160 11 84 376 1 288 3.40e-26 110
MS.gene30955.t1 MTR_1g100797 30.769 299 146 10 109 376 4 272 3.70e-25 107
MS.gene30955.t1 MTR_1g115575 31.220 205 122 4 14 215 134 322 1.33e-18 88.6
MS.gene30955.t1 MTR_7g117620 29.661 236 125 8 15 219 85 310 2.47e-17 84.7
MS.gene30955.t1 MTR_7g100390 31.429 245 131 9 5 219 41 278 3.36e-17 84.3
MS.gene30955.t1 MTR_2g033050 31.214 173 103 5 14 181 201 362 1.22e-15 79.0
MS.gene30955.t1 MTR_2g096190 29.796 245 133 9 5 218 58 294 5.80e-15 77.4
MS.gene30955.t1 MTR_1g089180 33.333 183 108 6 45 218 252 429 8.83e-15 76.6
MS.gene30955.t1 MTR_7g100430 30.332 211 125 5 11 215 120 314 6.82e-14 73.9
MS.gene30955.t1 MTR_4g062100 28.095 210 126 4 12 215 71 261 1.71e-13 72.8
MS.gene30955.t1 MTR_1g032100 26.471 238 142 9 11 232 53 273 2.94e-13 72.0
MS.gene30955.t1 MTR_7g115280 27.138 269 163 6 18 259 21 283 7.94e-13 70.5
MS.gene30955.t1 MTR_6g027840 25.974 231 140 4 25 240 141 355 3.45e-12 68.6
MS.gene30955.t1 MTR_6g027840 29.032 217 139 4 47 253 244 455 3.64e-12 68.6
MS.gene30955.t1 MTR_5g085830 33.742 163 98 6 18 177 282 437 7.62e-12 67.8
MS.gene30955.t1 MTR_5g023090 29.240 171 104 3 45 215 292 445 1.59e-11 66.6
MS.gene30955.t1 MTR_1g112830 26.471 238 150 8 15 247 139 356 4.53e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30955.t1 AT2G28840 67.483 449 133 4 1 438 1 447 0.0 625
MS.gene30955.t1 AT2G28840 70.976 379 97 4 71 438 57 433 0.0 559
MS.gene30955.t1 AT5G07270 31.658 398 215 10 1 373 1 366 4.17e-41 153
MS.gene30955.t1 AT5G57740 30.941 404 214 10 1 373 7 376 9.97e-39 147
MS.gene30955.t1 AT5G02620 29.021 286 164 13 12 277 57 323 3.71e-19 90.1
MS.gene30955.t1 AT5G02620 29.021 286 164 13 12 277 57 323 3.71e-19 90.1
MS.gene30955.t1 AT5G02620 29.021 286 164 13 12 277 57 323 3.71e-19 90.1
MS.gene30955.t1 AT3G04710 30.000 300 166 11 22 294 25 307 1.49e-17 85.1
MS.gene30955.t1 AT3G04710 30.000 300 166 11 22 294 249 531 1.57e-17 85.5
MS.gene30955.t1 AT5G02620 30.097 206 119 8 12 211 57 243 1.88e-17 85.1
MS.gene30955.t1 AT5G02620 30.097 206 119 8 12 211 57 243 1.88e-17 85.1
MS.gene30955.t1 AT3G12360 30.556 216 132 5 14 225 120 321 3.99e-17 84.3
MS.gene30955.t1 AT3G12360 30.556 216 132 5 14 225 132 333 4.92e-17 84.0
MS.gene30955.t1 AT5G60070 29.864 221 123 6 14 206 76 292 2.39e-16 81.6
MS.gene30955.t1 AT3G04710 30.000 300 165 12 22 294 25 306 2.53e-16 81.3
MS.gene30955.t1 AT1G07710 30.806 211 117 8 14 216 69 258 1.83e-15 79.0
MS.gene30955.t1 AT1G07710 30.806 211 117 8 14 216 69 258 1.83e-15 79.0
MS.gene30955.t1 AT2G31820 27.372 274 175 8 15 278 193 452 6.03e-14 74.3
MS.gene30955.t1 AT3G09550 29.064 203 127 5 15 215 147 334 1.99e-13 72.8
MS.gene30955.t1 AT3G09550 29.064 203 127 5 15 215 147 334 1.99e-13 72.8
MS.gene30955.t1 AT3G09550 29.064 203 127 5 15 215 154 341 2.07e-13 72.8
MS.gene30955.t1 AT5G20350 29.204 226 124 8 50 247 35 252 4.18e-13 71.6
MS.gene30955.t1 AT5G51160 29.139 151 92 4 37 180 34 176 3.12e-11 65.5

Find 93 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACACTATGCTGCTTATTA+TGG 0.234084 2.2:-5306434 MS.gene30955:CDS
CTAGTCATGTGGCTGCTTCT+TGG 0.245814 2.2:-5306370 MS.gene30955:intron
CAGAAGAAAGAATAGCTTTA+AGG 0.264068 2.2:+5306399 None:intergenic
ACTATGTTGCTTCGACAAAA+TGG 0.280496 2.2:+5304862 None:intergenic
TTGATGTTTGATACGGTTAA+TGG 0.291932 2.2:-5306467 MS.gene30955:CDS
TCTAACAAGGCTTTGGCTTC+CGG 0.299067 2.2:+5305427 None:intergenic
TACCGCTGTCGTTCAAATTT+CGG 0.312482 2.2:+5304743 None:intergenic
CTTGTTAGAACAAGCTTTGA+TGG 0.323868 2.2:-5305413 MS.gene30955:CDS
CGTATTCAGCGTGATTCATC+TGG 0.341209 2.2:-5305920 MS.gene30955:CDS
AAGTTGGGCGTCCGAAGTTC+AGG 0.350071 2.2:-5304682 MS.gene30955:CDS
CTTCATATTGCTGCTGCTAA+TGG 0.351267 2.2:-5306936 MS.gene30955:CDS
TGTGCTTCGACCGGTAGATA+TGG 0.358247 2.2:-5306127 MS.gene30955:CDS
AATAGAATATGTACACATTC+AGG 0.364281 2.2:+5306169 None:intergenic
AAGCAACATAGTTAGACTTA+TGG 0.364923 2.2:-5304851 MS.gene30955:CDS
TAGTGTGTCGTTCGGAAAGT+TGG 0.387276 2.2:-5304698 MS.gene30955:CDS
AGCTTGTTCTAACAAGGCTT+TGG 0.389126 2.2:+5305420 None:intergenic
CATCTGCTGAGCCTCTTGTT+TGG 0.394206 2.2:-5305483 MS.gene30955:CDS
CAATTTGAAGGTTACCTTGT+TGG 0.396080 2.2:+5307027 None:intergenic
GGGTGCGACGCCGTTACATT+TGG 0.413527 2.2:-5306209 MS.gene30955:CDS
ACAGAGTTGTTACAATTTGA+AGG 0.414832 2.2:+5307015 None:intergenic
CCCTTCATCTTGCTGCTAGA+GGG 0.415274 2.2:-5305988 MS.gene30955:CDS
TGATTGTATTCGTGAATTGT+TGG 0.428838 2.2:-5305957 MS.gene30955:CDS
AGTGTGTCGTTCGGAAAGTT+GGG 0.435757 2.2:-5304697 MS.gene30955:CDS
CTTCGACAAAATGGACAAAC+TGG 0.445468 2.2:+5304871 None:intergenic
TGCATTGCAGCCAACATAAG+AGG 0.455233 2.2:+5306628 None:intergenic
CCCCTTCATCTTGCTGCTAG+AGG 0.458363 2.2:-5305989 MS.gene30955:CDS
ATTCATCAGTGAACTTAATC+CGG 0.460718 2.2:-5305446 MS.gene30955:CDS
CAGGAAGGGTTGCTGCTGAA+TGG 0.461974 2.2:-5304663 MS.gene30955:CDS
AGATGAAGGGGAGTACTTCC+AGG 0.465349 2.2:+5306001 None:intergenic
TGATGGATGCAAACAGAGAA+AGG 0.466607 2.2:-5305396 MS.gene30955:CDS
AAGGGAGAAAGATATAATGA+AGG 0.467810 2.2:-5305377 MS.gene30955:CDS
GTGCTTCGACCGGTAGATAT+GGG 0.467851 2.2:-5306126 MS.gene30955:intron
CGGTTTGTTGTAGCAACAAA+GGG 0.467861 2.2:+5304921 None:intergenic
CCCCTCTAGCAGCAAGATGA+AGG 0.471186 2.2:+5305987 None:intergenic
TTGGTTGTAGGGGATTTGTT+CGG 0.476243 2.2:-5306255 MS.gene30955:intron
GAGTTATAGAAGTAGTTGTC+GGG 0.479062 2.2:+5304896 None:intergenic
ATAGAAGTAGTTGTCGGGTT+CGG 0.479189 2.2:+5304901 None:intergenic
TATTCGTGAATTGTTGGCAT+GGG 0.499232 2.2:-5305951 MS.gene30955:CDS
TGTTTCATAGCAACCATGTA+TGG 0.499705 2.2:+5305541 None:intergenic
CTGATGAATTTCAGTGGAGA+TGG 0.506025 2.2:+5305460 None:intergenic
AGGGAGAAAGATATAATGAA+GGG 0.507251 2.2:-5305376 MS.gene30955:CDS
GGAGTTATAGAAGTAGTTGT+CGG 0.511884 2.2:+5304895 None:intergenic
GCTCTTGTTTGTGCTTCGAC+CGG 0.514971 2.2:-5306136 MS.gene30955:CDS
ATGCTGCATCTGCTTTGAGC+AGG 0.517837 2.2:-5305001 MS.gene30955:CDS
CATCAAAGCTTGTTCTAACA+AGG 0.519785 2.2:+5305414 None:intergenic
CTTATGTTGGCTGCAATGCA+TGG 0.523935 2.2:-5306625 MS.gene30955:CDS
ATGTGCATTGTGCACACATT+TGG 0.527928 2.2:+5304947 None:intergenic
CTTCATCTTGCTGCTAGAGG+GGG 0.528164 2.2:-5305986 MS.gene30955:CDS
ACAATTGAAGTTCAGAATTG+TGG 0.536648 2.2:-5304973 MS.gene30955:CDS
TGCAGAGGTAACAATGATCA+TGG 0.538975 2.2:-5307065 MS.gene30955:CDS
CCCTCTAGCAGCAAGATGAA+GGG 0.539664 2.2:+5305988 None:intergenic
CCTTCATCTTGCTGCTAGAG+GGG 0.539952 2.2:-5305987 MS.gene30955:CDS
TTCTGCTCAATCTAGTCATG+TGG 0.540502 2.2:-5306381 MS.gene30955:CDS
TGTGAATGTTAGAGATGGAA+AGG 0.540835 2.2:-5306230 MS.gene30955:CDS
ATGCAGGCGTATACCATACA+TGG 0.545210 2.2:-5305554 MS.gene30955:intron
GGATTATCTAGTGTGTCGTT+CGG 0.546546 2.2:-5304706 MS.gene30955:CDS
TCGGTTTGTTGTAGCAACAA+AGG 0.550398 2.2:+5304920 None:intergenic
CTCTTCATTGCTGTCCAACA+AGG 0.550538 2.2:-5307041 MS.gene30955:CDS
CGGTTTGTGAATGTTAGAGA+TGG 0.552849 2.2:-5306235 MS.gene30955:CDS
GATGGATGCAAACAGAGAAA+GGG 0.553678 2.2:-5305395 MS.gene30955:CDS
CTCCCCGACAACATGGGTCA+AGG 0.556059 2.2:-5307095 None:intergenic
GTTCAAAGATAAACAAGTTG+AGG 0.560709 2.2:-5304771 MS.gene30955:CDS
CAGCAACCCTTCCTGAACTT+CGG 0.563206 2.2:+5304671 None:intergenic
AGCGGTAGTAGTAGCTTCAA+AGG 0.575054 2.2:-5304727 MS.gene30955:CDS
ACAAGAGGCTCAGCAGATGT+AGG 0.576412 2.2:+5305487 None:intergenic
GCATGGAAGGATTGCATGTG+TGG 0.582025 2.2:-5306608 MS.gene30955:CDS
TGTTGGCTGCAATGCATGGA+AGG 0.583065 2.2:-5306621 MS.gene30955:CDS
GTATTCGTGAATTGTTGGCA+TGG 0.584740 2.2:-5305952 MS.gene30955:CDS
GTGAATGTTAGAGATGGAAA+GGG 0.588584 2.2:-5306229 MS.gene30955:CDS
AGTTCACTGATGAATTTCAG+TGG 0.597323 2.2:+5305454 None:intergenic
TCTACACAATCTGATTGTGA+TGG 0.598122 2.2:+5305331 None:intergenic
GTGGAGAAGCTACTTGAAGC+TGG 0.601028 2.2:-5306589 MS.gene30955:CDS
TTCATCTTGCTGCTAGAGGG+GGG 0.602142 2.2:-5305985 MS.gene30955:CDS
ATTTGGCATCTCGACAAAGA+CGG 0.606723 2.2:-5306192 MS.gene30955:CDS
AGTGGAGATGGCCAAACAAG+AGG 0.607832 2.2:+5305472 None:intergenic
AGATCAGTACCCATATCTAC+CGG 0.613771 2.2:+5306117 None:intergenic
AGCAGATGTAGGATTTAGCA+AGG 0.613787 2.2:+5305498 None:intergenic
TGGGCGTCCGAAGTTCAGGA+AGG 0.616206 2.2:-5304678 MS.gene30955:CDS
GTATTCAGCGTGATTCATCT+GGG 0.618936 2.2:-5305919 MS.gene30955:intron
TGTCGAGATGCCAAATGTAA+CGG 0.619258 2.2:+5306199 None:intergenic
GGGCGTCCGAAGTTCAGGAA+GGG 0.620530 2.2:-5304677 MS.gene30955:CDS
CCTCTAGCAGCAAGATGAAG+GGG 0.627465 2.2:+5305989 None:intergenic
CAATCTGATTGTGATGGAGA+CGG 0.634456 2.2:+5305337 None:intergenic
CGACAAAATGGACAAACTGG+TGG 0.636725 2.2:+5304874 None:intergenic
TTAGCAGCAGCAATATGAAG+AGG 0.637344 2.2:+5306939 None:intergenic
GGTCAAGGTCTTAGTTGCAG+AGG 0.642603 2.2:-5307080 MS.gene30955:CDS
ATTCGTGAATTGTTGGCATG+GGG 0.650106 2.2:-5305950 MS.gene30955:CDS
GGGAGAAAGATATAATGAAG+GGG 0.658402 2.2:-5305375 MS.gene30955:CDS
TGCTGCTAATGGCCAGATCG+AGG 0.658793 2.2:-5306925 MS.gene30955:intron
CTCCGAAATTTGAACGACAG+CGG 0.698163 2.2:-5304745 MS.gene30955:CDS
GTACATATTCTATTAGACAG+TGG 0.720585 2.2:-5306160 MS.gene30955:CDS
GTTGCTATGAAACACAGACA+CGG 0.736330 2.2:-5305532 MS.gene30955:CDS
TGTAGATGACAATATCTCCG+AGG 0.741974 2.2:-5305314 MS.gene30955:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAATGTTTTTGAAT+TGG - chr2.2:5305457-5305476 MS.gene30955:CDS 10.0%
!!! AATTATTCTGAAAAAGAAAA+AGG + chr2.2:5305392-5305411 None:intergenic 15.0%
!!! TATAATGCAAATTTTAAACT+TGG + chr2.2:5306639-5306658 None:intergenic 15.0%
!! AAAAAACACAATGCAAAATA+AGG + chr2.2:5305018-5305037 None:intergenic 20.0%
!! AAATTCAGTTCAAATTCATT+TGG + chr2.2:5306552-5306571 None:intergenic 20.0%
!! ATAATTCATCTTCATTTATG+TGG - chr2.2:5305872-5305891 MS.gene30955:intron 20.0%
!! ATTGTAACTTCTCAAATTTA+AGG - chr2.2:5306030-5306049 MS.gene30955:intron 20.0%
!! TGTTAATCCATATATGAATT+TGG - chr2.2:5305204-5305223 MS.gene30955:intron 20.0%
!! TTATCATTTGTTTCAATCAT+AGG - chr2.2:5306592-5306611 MS.gene30955:CDS 20.0%
!!! AATTCTTTTTCCTTATTTTC+TGG - chr2.2:5304831-5304850 MS.gene30955:CDS 20.0%
!!! CAATTTTCCAAATTCATATA+TGG + chr2.2:5305214-5305233 None:intergenic 20.0%
!!! TGTTTTAAATCGAAAAAAAC+AGG - chr2.2:5304975-5304994 MS.gene30955:CDS 20.0%
!!! TTATTTTGCATTGTGTTTTT+TGG - chr2.2:5305017-5305036 MS.gene30955:CDS 20.0%
!!! TTTTTTCGATTTAAAACATC+TGG + chr2.2:5304973-5304992 None:intergenic 20.0%
! AAATCGAAAAAAACAGGTAA+TGG - chr2.2:5304981-5305000 MS.gene30955:CDS 25.0%
! AATAGAATATGTACACATTC+AGG + chr2.2:5305565-5305584 None:intergenic 25.0%
! AATTGCAAAACACAAGAATT+TGG + chr2.2:5306480-5306499 None:intergenic 25.0%
! AGGGATAAAATAATTGATGA+GGG + chr2.2:5306131-5306150 None:intergenic 25.0%
! GAAAAAAGGGTTAATCAAAA+AGG + chr2.2:5305078-5305097 None:intergenic 25.0%
! TAGGGATAAAATAATTGATG+AGG + chr2.2:5306132-5306151 None:intergenic 25.0%
!! ATCAAACATCTAAGTCTTTT+AGG - chr2.2:5305948-5305967 MS.gene30955:CDS 25.0%
!! ATGGATTGTAGCATTTAATT+AGG - chr2.2:5306095-5306114 MS.gene30955:intron 25.0%
!!! AATGTTTTTGAATTGGTTGT+AGG - chr2.2:5305464-5305483 MS.gene30955:CDS 25.0%
!!! ATGTTTTTGAATTGGTTGTA+GGG - chr2.2:5305465-5305484 MS.gene30955:CDS 25.0%
!!! TCAGAATAATTCTTTTTTCC+TGG - chr2.2:5305401-5305420 MS.gene30955:CDS 25.0%
!!! TGAGAATGCTATTTCTTTTT+TGG + chr2.2:5306670-5306689 None:intergenic 25.0%
!!! TTAGCTGTTTTAAAGTTGAA+TGG - chr2.2:5306076-5306095 MS.gene30955:intron 25.0%
AAGAAATAGCATTCTCATTG+TGG - chr2.2:5306673-5306692 MS.gene30955:intron 30.0%
AAGCAACATAGTTAGACTTA+TGG - chr2.2:5306880-5306899 MS.gene30955:intron 30.0%
AAGGGAGAAAGATATAATGA+AGG - chr2.2:5306354-5306373 MS.gene30955:intron 30.0%
ACAATTGAAGTTCAGAATTG+TGG - chr2.2:5306758-5306777 MS.gene30955:CDS 30.0%
ACAGAGTTGTTACAATTTGA+AGG + chr2.2:5304719-5304738 None:intergenic 30.0%
ACCAAACAACATGAAAATCT+AGG + chr2.2:5306151-5306170 None:intergenic 30.0%
ACTGATCTCTCAATATTCTA+TGG - chr2.2:5305629-5305648 MS.gene30955:intron 30.0%
AGGGAGAAAGATATAATGAA+GGG - chr2.2:5306355-5306374 MS.gene30955:intron 30.0%
ATAAGAGGAGTCTGAAAAAA+GGG + chr2.2:5305091-5305110 None:intergenic 30.0%
ATTCATCAGTGAACTTAATC+CGG - chr2.2:5306285-5306304 MS.gene30955:intron 30.0%
CCAAACAACATGAAAATCTA+GGG + chr2.2:5306150-5306169 None:intergenic 30.0%
GAATTTGGAAAATTGATGTG+TGG - chr2.2:5305219-5305238 MS.gene30955:intron 30.0%
GGGATAAAATAATTGATGAG+GGG + chr2.2:5306130-5306149 None:intergenic 30.0%
GTACATATTCTATTAGACAG+TGG - chr2.2:5305571-5305590 MS.gene30955:intron 30.0%
GTTCAAAGATAAACAAGTTG+AGG - chr2.2:5306960-5306979 MS.gene30955:CDS 30.0%
TCTTCATTTATGTGGCTTAT+TGG - chr2.2:5305880-5305899 MS.gene30955:intron 30.0%
TGATTGTATTCGTGAATTGT+TGG - chr2.2:5305774-5305793 MS.gene30955:intron 30.0%
TGGAAACTATGAAAATCTTC+AGG - chr2.2:5305239-5305258 MS.gene30955:intron 30.0%
TGTGGAATATGATATTATGC+AGG - chr2.2:5306691-5306710 MS.gene30955:intron 30.0%
TTGATGTTTGATACGGTTAA+TGG - chr2.2:5305264-5305283 MS.gene30955:intron 30.0%
! CAGAAGAAAGAATAGCTTTA+AGG + chr2.2:5305335-5305354 None:intergenic 30.0%
! TTACACTATGCTGCTTATTA+TGG - chr2.2:5305297-5305316 MS.gene30955:intron 30.0%
!! TAGCTTTAAGGCAAGAAAAA+TGG + chr2.2:5305323-5305342 None:intergenic 30.0%
!! TCAGGTTTTGATGTTTGATA+CGG - chr2.2:5305257-5305276 MS.gene30955:intron 30.0%
!! TGTTTTTGAATTGGTTGTAG+GGG - chr2.2:5305466-5305485 MS.gene30955:CDS 30.0%
!! TTTTGTTTCAGATTCTGTCA+AGG - chr2.2:5304926-5304945 MS.gene30955:CDS 30.0%
!!! AACGATCAAAGAGAGTTTTT+TGG + chr2.2:5304767-5304786 None:intergenic 30.0%
!!! TTTTTTGGTGGAAAAGAGAA+GGG + chr2.2:5304752-5304771 None:intergenic 30.0%
AAAAAGAATTCACCTCGATC+TGG + chr2.2:5304821-5304840 None:intergenic 35.0%
AGCAGAAATGCCAGAAAATA+AGG + chr2.2:5304844-5304863 None:intergenic 35.0%
AGTTCACTGATGAATTTCAG+TGG + chr2.2:5306280-5306299 None:intergenic 35.0%
ATCAAAGTCAAAGTCAATCC+AGG + chr2.2:5305422-5305441 None:intergenic 35.0%
CAAAAGGAGTCAAATTACCT+CGG + chr2.2:5306437-5306456 None:intergenic 35.0%
CAATTTGAAGGTTACCTTGT+TGG + chr2.2:5304707-5304726 None:intergenic 35.0%
CATAAGAGGAGTCTGAAAAA+AGG + chr2.2:5305092-5305111 None:intergenic 35.0%
GAGTTATAGAAGTAGTTGTC+GGG + chr2.2:5306838-5306857 None:intergenic 35.0%
GGAGTTATAGAAGTAGTTGT+CGG + chr2.2:5306839-5306858 None:intergenic 35.0%
GGCTGAGAGATTTGATTATT+TGG - chr2.2:5306613-5306632 MS.gene30955:CDS 35.0%
GGGAGAAAGATATAATGAAG+GGG - chr2.2:5306356-5306375 MS.gene30955:intron 35.0%
GTGAATGTTAGAGATGGAAA+GGG - chr2.2:5305502-5305521 MS.gene30955:CDS 35.0%
TATTCGTGAATTGTTGGCAT+GGG - chr2.2:5305780-5305799 MS.gene30955:intron 35.0%
TCTACACAATCTGATTGTGA+TGG + chr2.2:5306403-5306422 None:intergenic 35.0%
TGTGAATGTTAGAGATGGAA+AGG - chr2.2:5305501-5305520 MS.gene30955:CDS 35.0%
TGTTTCATAGCAACCATGTA+TGG + chr2.2:5306193-5306212 None:intergenic 35.0%
! AAGCTGGTGCTAATGTAATT+TGG - chr2.2:5305158-5305177 MS.gene30955:intron 35.0%
! ACTATGTTGCTTCGACAAAA+TGG + chr2.2:5306872-5306891 None:intergenic 35.0%
! ATTCTGTCAAGGCTTTTACA+TGG - chr2.2:5304937-5304956 MS.gene30955:CDS 35.0%
! CATCAAAGCTTGTTCTAACA+AGG + chr2.2:5306320-5306339 None:intergenic 35.0%
! CCCTAGATTTTCATGTTGTT+TGG - chr2.2:5306147-5306166 MS.gene30955:CDS 35.0%
! CGAAAAAAACAGGTAATGGT+TGG - chr2.2:5304985-5305004 MS.gene30955:CDS 35.0%
! CTTGTTAGAACAAGCTTTGA+TGG - chr2.2:5306318-5306337 MS.gene30955:intron 35.0%
! TTGAAATTTTGCAGCTATCC+TGG - chr2.2:5305712-5305731 MS.gene30955:intron 35.0%
! TTTTCAGACTCCTCTTATGT+TGG - chr2.2:5305093-5305112 MS.gene30955:intron 35.0%
!! GTGGCTTATTGGTTTGTATA+TGG - chr2.2:5305891-5305910 MS.gene30955:intron 35.0%
!!! CATTGTGTTTTTTGGCATGT+TGG - chr2.2:5305025-5305044 MS.gene30955:intron 35.0%
!!! GATCAAAGAGAGTTTTTTGG+TGG + chr2.2:5304764-5304783 None:intergenic 35.0%
!!! GTTTTTTGGTGGAAAAGAGA+AGG + chr2.2:5304753-5304772 None:intergenic 35.0%
AGATCAGTACCCATATCTAC+CGG + chr2.2:5305617-5305636 None:intergenic 40.0%
AGCAGATGTAGGATTTAGCA+AGG + chr2.2:5306236-5306255 None:intergenic 40.0%
ATAGAAGTAGTTGTCGGGTT+CGG + chr2.2:5306833-5306852 None:intergenic 40.0%
ATGTGCATTGTGCACACATT+TGG + chr2.2:5306787-5306806 None:intergenic 40.0%
ATTCGTGAATTGTTGGCATG+GGG - chr2.2:5305781-5305800 MS.gene30955:intron 40.0%
ATTTGGCATCTCGACAAAGA+CGG - chr2.2:5305539-5305558 MS.gene30955:CDS 40.0%
CGGTTTGTGAATGTTAGAGA+TGG - chr2.2:5305496-5305515 MS.gene30955:CDS 40.0%
CTGATGAATTTCAGTGGAGA+TGG + chr2.2:5306274-5306293 None:intergenic 40.0%
CTTCATATTGCTGCTGCTAA+TGG - chr2.2:5304795-5304814 MS.gene30955:CDS 40.0%
CTTCGACAAAATGGACAAAC+TGG + chr2.2:5306863-5306882 None:intergenic 40.0%
GATGGATGCAAACAGAGAAA+GGG - chr2.2:5306336-5306355 MS.gene30955:intron 40.0%
GTATTCAGCGTGATTCATCT+GGG - chr2.2:5305812-5305831 MS.gene30955:intron 40.0%
GTATTCGTGAATTGTTGGCA+TGG - chr2.2:5305779-5305798 MS.gene30955:intron 40.0%
GTTGCTATGAAACACAGACA+CGG - chr2.2:5306199-5306218 MS.gene30955:CDS 40.0%
TACCGCTGTCGTTCAAATTT+CGG + chr2.2:5306991-5307010 None:intergenic 40.0%
TGCAGAGGTAACAATGATCA+TGG - chr2.2:5304666-5304685 MS.gene30955:CDS 40.0%
TGTAGATGACAATATCTCCG+AGG - chr2.2:5306417-5306436 MS.gene30955:CDS 40.0%
TGTCGAGATGCCAAATGTAA+CGG + chr2.2:5305535-5305554 None:intergenic 40.0%
TTAGCAGCAGCAATATGAAG+AGG + chr2.2:5304795-5304814 None:intergenic 40.0%
TTCTGCTCAATCTAGTCATG+TGG - chr2.2:5305350-5305369 MS.gene30955:CDS 40.0%
! AGCTTGTTCTAACAAGGCTT+TGG + chr2.2:5306314-5306333 None:intergenic 40.0%
! CAATCTGATTGTGATGGAGA+CGG + chr2.2:5306397-5306416 None:intergenic 40.0%
! GGATTATCTAGTGTGTCGTT+CGG - chr2.2:5307025-5307044 MS.gene30955:CDS 40.0%
! TGATGGATGCAAACAGAGAA+AGG - chr2.2:5306335-5306354 MS.gene30955:intron 40.0%
! TTGGTTGTAGGGGATTTGTT+CGG - chr2.2:5305476-5305495 MS.gene30955:CDS 40.0%
!! CGGTTTGTTGTAGCAACAAA+GGG + chr2.2:5306813-5306832 None:intergenic 40.0%
!! TCGGTTTGTTGTAGCAACAA+AGG + chr2.2:5306814-5306833 None:intergenic 40.0%
AGCGGTAGTAGTAGCTTCAA+AGG - chr2.2:5307004-5307023 MS.gene30955:CDS 45.0%
ATGCAGGCGTATACCATACA+TGG - chr2.2:5306177-5306196 MS.gene30955:CDS 45.0%
CGACAAAATGGACAAACTGG+TGG + chr2.2:5306860-5306879 None:intergenic 45.0%
CGTATTCAGCGTGATTCATC+TGG - chr2.2:5305811-5305830 MS.gene30955:intron 45.0%
CTCCGAAATTTGAACGACAG+CGG - chr2.2:5306986-5307005 MS.gene30955:CDS 45.0%
CTCTTCATTGCTGTCCAACA+AGG - chr2.2:5304690-5304709 MS.gene30955:CDS 45.0%
TGCATTGCAGCCAACATAAG+AGG + chr2.2:5305106-5305125 None:intergenic 45.0%
! AGTGTGTCGTTCGGAAAGTT+GGG - chr2.2:5307034-5307053 MS.gene30955:CDS 45.0%
! CTTATGTTGGCTGCAATGCA+TGG - chr2.2:5305106-5305125 MS.gene30955:intron 45.0%
! TAGTGTGTCGTTCGGAAAGT+TGG - chr2.2:5307033-5307052 MS.gene30955:CDS 45.0%
! TCTAACAAGGCTTTGGCTTC+CGG + chr2.2:5306307-5306326 None:intergenic 45.0%
!! GTTGTTTGGTGCGTTTATGC+AGG - chr2.2:5306161-5306180 MS.gene30955:CDS 45.0%
ACAAGAGGCTCAGCAGATGT+AGG + chr2.2:5306247-5306266 None:intergenic 50.0%
AGTGGAGATGGCCAAACAAG+AGG + chr2.2:5306262-5306281 None:intergenic 50.0%
CAGCAACCCTTCCTGAACTT+CGG + chr2.2:5307063-5307082 None:intergenic 50.0%
CAGCGTGATTCATCTGGGTA+TGG - chr2.2:5305817-5305836 MS.gene30955:intron 50.0%
CATCTGCTGAGCCTCTTGTT+TGG - chr2.2:5306248-5306267 MS.gene30955:CDS 50.0%
CCCTCTAGCAGCAAGATGAA+GGG + chr2.2:5305746-5305765 None:intergenic 50.0%
CCCTTCATCTTGCTGCTAGA+GGG - chr2.2:5305743-5305762 MS.gene30955:intron 50.0%
CCTCTAGCAGCAAGATGAAG+GGG + chr2.2:5305745-5305764 None:intergenic 50.0%
CCTTCATCTTGCTGCTAGAG+GGG - chr2.2:5305744-5305763 MS.gene30955:intron 50.0%
CTAGTCATGTGGCTGCTTCT+TGG - chr2.2:5305361-5305380 MS.gene30955:CDS 50.0%
CTTCATCTTGCTGCTAGAGG+GGG - chr2.2:5305745-5305764 MS.gene30955:intron 50.0%
GCATGGAAGGATTGCATGTG+TGG - chr2.2:5305123-5305142 MS.gene30955:intron 50.0%
GGTCAAGGTCTTAGTTGCAG+AGG - chr2.2:5304651-5304670 MS.gene30955:CDS 50.0%
GTGCTTCGACCGGTAGATAT+GGG - chr2.2:5305605-5305624 MS.gene30955:intron 50.0%
GTGGAGAAGCTACTTGAAGC+TGG - chr2.2:5305142-5305161 MS.gene30955:intron 50.0%
TATGTGTGCACGCGGACAAA+AGG + chr2.2:5306453-5306472 None:intergenic 50.0%
TGTGCTTCGACCGGTAGATA+TGG - chr2.2:5305604-5305623 MS.gene30955:intron 50.0%
TTCATCTTGCTGCTAGAGGG+GGG - chr2.2:5305746-5305765 MS.gene30955:intron 50.0%
TTGGTGTGTATGTGTGCACG+CGG + chr2.2:5306461-5306480 None:intergenic 50.0%
! ATGCTGCATCTGCTTTGAGC+AGG - chr2.2:5306730-5306749 MS.gene30955:intron 50.0%
! GCTCTTGTTTGTGCTTCGAC+CGG - chr2.2:5305595-5305614 MS.gene30955:intron 50.0%
! TGTTGGCTGCAATGCATGGA+AGG - chr2.2:5305110-5305129 MS.gene30955:intron 50.0%
!! AGATGAAGGGGAGTACTTCC+AGG + chr2.2:5305733-5305752 None:intergenic 50.0%
AAGTTGGGCGTCCGAAGTTC+AGG - chr2.2:5307049-5307068 MS.gene30955:CDS 55.0%
CAGGAAGGGTTGCTGCTGAA+TGG - chr2.2:5307068-5307087 MS.gene30955:CDS 55.0%
CCCCTCTAGCAGCAAGATGA+AGG + chr2.2:5305747-5305766 None:intergenic 55.0%
CCCCTTCATCTTGCTGCTAG+AGG - chr2.2:5305742-5305761 MS.gene30955:intron 55.0%
TGCTGCTAATGGCCAGATCG+AGG - chr2.2:5304806-5304825 MS.gene30955:CDS 55.0%
GGGCGTCCGAAGTTCAGGAA+GGG - chr2.2:5307054-5307073 MS.gene30955:CDS 60.0%
GGGTGCGACGCCGTTACATT+TGG - chr2.2:5305522-5305541 MS.gene30955:CDS 60.0%
TGGGCGTCCGAAGTTCAGGA+AGG - chr2.2:5307053-5307072 MS.gene30955:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 5304648 5307105 5304648 ID=MS.gene30955
chr2.2 mRNA 5304648 5307105 5304648 ID=MS.gene30955.t1;Parent=MS.gene30955
chr2.2 exon 5306926 5307105 5306926 ID=MS.gene30955.t1.exon1;Parent=MS.gene30955.t1
chr2.2 CDS 5306926 5307105 5306926 ID=cds.MS.gene30955.t1;Parent=MS.gene30955.t1
chr2.2 exon 5306757 5306816 5306757 ID=MS.gene30955.t1.exon2;Parent=MS.gene30955.t1
chr2.2 CDS 5306757 5306816 5306757 ID=cds.MS.gene30955.t1;Parent=MS.gene30955.t1
chr2.2 exon 5306582 5306653 5306582 ID=MS.gene30955.t1.exon3;Parent=MS.gene30955.t1
chr2.2 CDS 5306582 5306653 5306582 ID=cds.MS.gene30955.t1;Parent=MS.gene30955.t1
chr2.2 exon 5306371 5306492 5306371 ID=MS.gene30955.t1.exon4;Parent=MS.gene30955.t1
chr2.2 CDS 5306371 5306492 5306371 ID=cds.MS.gene30955.t1;Parent=MS.gene30955.t1
chr2.2 exon 5306127 5306267 5306127 ID=MS.gene30955.t1.exon5;Parent=MS.gene30955.t1
chr2.2 CDS 5306127 5306267 5306127 ID=cds.MS.gene30955.t1;Parent=MS.gene30955.t1
chr2.2 exon 5305920 5306027 5305920 ID=MS.gene30955.t1.exon6;Parent=MS.gene30955.t1
chr2.2 CDS 5305920 5306027 5305920 ID=cds.MS.gene30955.t1;Parent=MS.gene30955.t1
chr2.2 exon 5305315 5305570 5305315 ID=MS.gene30955.t1.exon7;Parent=MS.gene30955.t1
chr2.2 CDS 5305315 5305570 5305315 ID=cds.MS.gene30955.t1;Parent=MS.gene30955.t1
chr2.2 exon 5304648 5305040 5304648 ID=MS.gene30955.t1.exon8;Parent=MS.gene30955.t1
chr2.2 CDS 5304648 5305040 5304648 ID=cds.MS.gene30955.t1;Parent=MS.gene30955.t1
Gene Sequence

>MS.gene30955

ATGGGTCAAGGTCTTAGTTGCAGAGGTAACAATGATCATGGTCTCTTCATTGCTGTCCAACAAGGTAACCTTCAAATTGTAACAACTCTGTTACAACAAGACCCTTCTCTTTTCCACCAAAAAACTCTCTTTGATCGTTTTTCACCTCTTCATATTGCTGCTGCTAATGGCCAGATCGAGGTGAATTCTTTTTCCTTATTTTCTGGCATTTCTGCTAAGTTTAGTTTAAGAATTAATATTCTATTTTGTTTTGTTTGTAACATTTCTGTTTTTTTTTGTTTTGTTTCAGATTCTGTCAAGGCTTTTACATGGATCTGTTAATCCAGATGTTTTAAATCGAAAAAAACAGGTAATGGTTGGTTATGTTCCTTATTTTGCATTGTGTTTTTTGGCATGTTGGTTTTTGTTTTTGTTGCTAAAAGTGTTACCTTTTTGATTAACCCTTTTTTCAGACTCCTCTTATGTTGGCTGCAATGCATGGAAGGATTGCATGTGTGGAGAAGCTACTTGAAGCTGGTGCTAATGTAATTTGGAAAATTTCATTTTTAGTTTCATTTGTTAATCCATATATGAATTTGGAAAATTGATGTGTGGAAACTATGAAAATCTTCAGGTTTTGATGTTTGATACGGTTAATGGAAGAACTTGTTTACACTATGCTGCTTATTATGGCCATTTTTCTTGCCTTAAAGCTATTCTTTCTTCTGCTCAATCTAGTCATGTGGCTGCTTCTTGGTTTGTCCTTTTTCTTTTTCAGAATAATTCTTTTTTCCTGGATTGACTTTGACTTTGATGCTATGCTGTTGTTGTTTTTTAAATGTTTTTGAATTGGTTGTAGGGGATTTGTTCGGTTTGTGAATGTTAGAGATGGAAAGGGTGCGACGCCGTTACATTTGGCATCTCGACAAAGACGGCCTGAATGTGTACATATTCTATTAGACAGTGGTGCTCTTGTTTGTGCTTCGACCGGTAGATATGGGTACTGATCTCTCAATATTCTATGGTTAATTAAAATGACTTGAATCTTATGCATTCTTCTGTTTTGTGTACTAATGAATTGTTATTTGAAATTTTGCAGCTATCCTGGAAGTACTCCCCTTCATCTTGCTGCTAGAGGGGGGTCTCTTGATTGTATTCGTGAATTGTTGGCATGGGGCGCAGATCGTATTCAGCGTGATTCATCTGGGTATGGTTTGATTTTGAGACTTGTTAACATATCTAAACATAATTCATCTTCATTTATGTGGCTTATTGGTTTGTATATGGATTATTTATGTTAATGTGTTGAAGTTATTGTAATATCAAACATCTAAGTCTTTTAGGCAAATCTTTGATATGATAATTTCATAATTTGTTTTAATACTCATATCTAGCATTTTCTTATTGTAACTTCTCAAATTTAAGGAGAAACTTTCTTTATTTAACGATTTAGCTGTTTTAAAGTTGAATGGATTGTAGCATTTAATTAGGCATTTTTTTCCCCTCATCAATTATTTTATCCCTAGATTTTCATGTTGTTTGGTGCGTTTATGCAGGCGTATACCATACATGGTTGCTATGAAACACAGACACGGATCATGCACGTCCTTGCTAAATCCTACATCTGCTGAGCCTCTTGTTTGGCCATCTCCACTGAAATTCATCAGTGAACTTAATCCGGAAGCCAAAGCCTTGTTAGAACAAGCTTTGATGGATGCAAACAGAGAAAGGGAGAAAGATATAATGAAGGGGAAATTTTACTCTCTTCCGTCTCCATCACAATCAGATTGTGTAGATGACAATATCTCCGAGGTAATTTGACTCCTTTTGTCCGCGTGCACACATACACACCAAATTCTTGTGTTTTGCAATTGAATTAATCATAATGCTGAAATGTTGCTTATTTGTTATGTGTATACAAACCAAATGAATTTGAACTGAATTTTTGTGATATGCTATCTGAATTTATCATTTGTTTCAATCATAGGCTGAGAGATTTGATTATTTGGCCAAGTTTAAAATTTGCATTATAAATGTGATCCAAAAAAGAAATAGCATTCTCATTGTGGAATATGATATTATGCAGGTCAGTGAGTCAGAATTATGCTGCATCTGCTTTGAGCAGGTATGCACAATTGAAGTTCAGAATTGTGGTCACCAAATGTGTGCACAATGCACATTATCCCTTTGTTGCTACAACAAACCGAACCCGACAACTACTTCTATAACTCCACCAGTTTGTCCATTTTGTCGAAGCAACATAGTTAGACTTATGGTTGTCATGAAAATTGAAAATCACGACGAAACTGATCAAGACAACGTTGATATCAATTGTTCAAAGATAAACAAGTTGAGGAAACTCCGAAATTTGAACGACAGCGGTAGTAGTAGCTTCAAAGGATTATCTAGTGTGTCGTTCGGAAAGTTGGGCGTCCGAAGTTCAGGAAGGGTTGCTGCTGAATGGATTGATAAAGAATGA
Protein sequence

>MS.gene30955.t1

MGQGLSCRGNNDHGLFIAVQQGNLQIVTTLLQQDPSLFHQKTLFDRFSPLHIAAANGQIEILSRLLHGSVNPDVLNRKKQTPLMLAAMHGRIACVEKLLEAGANVLMFDTVNGRTCLHYAAYYGHFSCLKAILSSAQSSHVAASWGFVRFVNVRDGKGATPLHLASRQRRPECVHILLDSGALVCASTGRYGYPGSTPLHLAARGGSLDCIRELLAWGADRIQRDSSGRIPYMVAMKHRHGSCTSLLNPTSAEPLVWPSPLKFISELNPEAKALLEQALMDANREREKDIMKGKFYSLPSPSQSDCVDDNISEVSESELCCICFEQVCTIEVQNCGHQMCAQCTLSLCCYNKPNPTTTSITPPVCPFCRSNIVRLMVVMKIENHDETDQDNVDINCSKINKLRKLRNLNDSGSSSFKGLSSVSFGKLGVRSSGRVAAEWIDKE