AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26098


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26098.t1 MTR_1g007860 59.756 82 1 1 1 50 144 225 1.32e-23 92.4
MS.gene26098.t1 MTR_8g093450 92.308 39 3 0 51 89 57 95 9.06e-20 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26098.t1 AT5G53050 47.561 82 11 1 1 50 132 213 1.28e-16 72.8
MS.gene26098.t1 AT5G53050 47.561 82 11 1 1 50 132 213 1.29e-16 72.8
MS.gene26098.t1 AT5G53050 47.561 82 11 1 1 50 132 213 1.84e-16 72.4
MS.gene26098.t1 AT5G53050 47.561 82 11 1 1 50 132 213 1.91e-16 72.8
MS.gene26098.t1 AT5G53050 47.561 82 11 1 1 50 132 213 1.91e-16 72.8
MS.gene26098.t1 AT5G53050 47.561 82 11 1 1 50 132 213 3.04e-16 72.4

Find 23 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAAAGATGAAAAGTGTA+TGG 0.395163 5.4:+56540087 MS.gene26098:CDS
GTGTTGTACCTGATACAATA+TGG 0.399867 5.4:-56539919 None:intergenic
GTGAAAGATAAGAAAGATCT+TGG 0.408538 5.4:+56540002 MS.gene26098:CDS
TTGCTCAATGCAACAGGTGG+TGG 0.415046 5.4:+56538676 MS.gene26098:CDS
GAGGAGAACCATATTGTATC+AGG 0.416982 5.4:+56539911 MS.gene26098:CDS
GCCATGGAGAGAACTCTATC+AGG 0.426456 5.4:-56538652 None:intergenic
ATGGCCTTGCTCAATGCAAC+AGG 0.435885 5.4:+56538670 MS.gene26098:CDS
AGCTTGTAAATTTGCAGCAA+TGG 0.448455 5.4:+56538627 MS.gene26098:CDS
GTTTAACAAAGAGGAGGGAT+TGG 0.467919 5.4:+56540055 MS.gene26098:CDS
CAATATGGTTCTCCTCTTCA+CGG 0.490112 5.4:-56539904 None:intergenic
GAATATCTTGAGGAATATGT+TGG 0.532265 5.4:+56539879 MS.gene26098:CDS
TGTTGCATTGAGCAAGGCCA+TGG 0.536559 5.4:-56538668 None:intergenic
AGAAAAGATGAAAAGTGTAT+GGG 0.540681 5.4:+56540088 MS.gene26098:CDS
TCCTGATAGAGTTCTCTCCA+TGG 0.577551 5.4:+56538651 MS.gene26098:CDS
TATCAGTTTAACAAAGAGGA+GGG 0.577814 5.4:+56540050 MS.gene26098:CDS
GTATTATCAGTTTAACAAAG+AGG 0.579681 5.4:+56540046 MS.gene26098:CDS
TCTTGGAATCATGAAAGTCA+AGG 0.609272 5.4:+56540019 MS.gene26098:CDS
TTATCAGTTTAACAAAGAGG+AGG 0.617782 5.4:+56540049 MS.gene26098:CDS
ACCACCTGTTGCATTGAGCA+AGG 0.621860 5.4:-56538674 None:intergenic
TCACGTGCAGGAATATCTTG+AGG 0.635000 5.4:+56539869 MS.gene26098:intron
AAGATGAAAAGTGTATGGGA+AGG 0.640402 5.4:+56540092 MS.gene26098:CDS
AATATGTTGGAACCGTGAAG+AGG 0.650450 5.4:+56539892 MS.gene26098:CDS
GCCTTGCTCAATGCAACAGG+TGG 0.684486 5.4:+56538673 MS.gene26098:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTTTCAATTTTTTTTTA+CGG + chr5.4:56538753-56538772 MS.gene26098:intron 10.0%
!! ATGCTTAATACAAATTTAAT+TGG + chr5.4:56539289-56539308 MS.gene26098:intron 15.0%
!! ATTAAATTTGTATTAAGCAT+TGG - chr5.4:56539289-56539308 None:intergenic 15.0%
!!! TTTTAAAATCACTACTTTAA+TGG - chr5.4:56539645-56539664 None:intergenic 15.0%
!! TGCAATTAGATATATAATGT+TGG + chr5.4:56539320-56539339 MS.gene26098:intron 20.0%
!!! AATTTCTTACTACTGATTTT+AGG + chr5.4:56539088-56539107 MS.gene26098:intron 20.0%
! AATGGTAAGTTTAATATAGC+TGG - chr5.4:56539627-56539646 None:intergenic 25.0%
! ACATTTCACAGATTTATACA+AGG - chr5.4:56538738-56538757 None:intergenic 25.0%
! ATGGTAAGTTTAATATAGCT+GGG - chr5.4:56539626-56539645 None:intergenic 25.0%
! CATTTCACAGATTTATACAA+GGG - chr5.4:56538737-56538756 None:intergenic 25.0%
! GTATATGATATAAAAGAGTC+TGG + chr5.4:56539689-56539708 MS.gene26098:intron 25.0%
! GTATTATCAGTTTAACAAAG+AGG + chr5.4:56540046-56540065 MS.gene26098:CDS 25.0%
! TAATATAGAAGAGAAAGTCA+CGG + chr5.4:56538955-56538974 MS.gene26098:intron 25.0%
! TTTAATTACAAACGTAGTTC+AGG + chr5.4:56539037-56539056 MS.gene26098:intron 25.0%
!! AAATTTTATATATCACGTGC+AGG + chr5.4:56539857-56539876 MS.gene26098:intron 25.0%
!! AAGAAAAGATGAAAAGTGTA+TGG + chr5.4:56540087-56540106 MS.gene26098:CDS 25.0%
!! AGAAAAGATGAAAAGTGTAT+GGG + chr5.4:56540088-56540107 MS.gene26098:CDS 25.0%
!!! TATCTTGTTTTTTCAGTCTT+GGG + chr5.4:56539755-56539774 MS.gene26098:intron 25.0%
AACTAACTACATTGGGAATT+GGG + chr5.4:56538823-56538842 MS.gene26098:intron 30.0%
ATCTTTCTTATCTTTCACCT+TGG - chr5.4:56540000-56540019 None:intergenic 30.0%
CTAAATTTCTCCTAAAGGTT+TGG - chr5.4:56539154-56539173 None:intergenic 30.0%
CTCAACTAAATTTCTCCTAA+AGG - chr5.4:56539159-56539178 None:intergenic 30.0%
CTGAAAAGAAGCTTCAATTT+AGG + chr5.4:56539945-56539964 MS.gene26098:intron 30.0%
GAAGTTGCATACATAACAAA+TGG + chr5.4:56539065-56539084 MS.gene26098:intron 30.0%
GAATATCTTGAGGAATATGT+TGG + chr5.4:56539879-56539898 MS.gene26098:CDS 30.0%
GCTAGTTAACTAACTACATT+GGG + chr5.4:56538816-56538835 MS.gene26098:intron 30.0%
GGTTTCCTATGAACTATTAT+CGG + chr5.4:56539371-56539390 MS.gene26098:intron 30.0%
GTCACATAATATAGTACCTT+TGG - chr5.4:56538715-56538734 None:intergenic 30.0%
GTGAAAGATAAGAAAGATCT+TGG + chr5.4:56540002-56540021 MS.gene26098:CDS 30.0%
TAACTAACTACATTGGGAAT+TGG + chr5.4:56538822-56538841 MS.gene26098:intron 30.0%
TAGAACAAGAACCTCTTATA+AGG - chr5.4:56539428-56539447 None:intergenic 30.0%
TATCAGTTTAACAAAGAGGA+GGG + chr5.4:56540050-56540069 MS.gene26098:CDS 30.0%
TCTTTCTTATCTTTCACCTT+GGG - chr5.4:56539999-56540018 None:intergenic 30.0%
TGGTAAGTTTAATATAGCTG+GGG - chr5.4:56539625-56539644 None:intergenic 30.0%
TGTGTTAATGTGCTATCTTT+AGG + chr5.4:56539819-56539838 MS.gene26098:intron 30.0%
TTATCAGTTTAACAAAGAGG+AGG + chr5.4:56540049-56540068 MS.gene26098:CDS 30.0%
! TGGTTTTCAATGTTTTCCAA+AGG + chr5.4:56538696-56538715 MS.gene26098:CDS 30.0%
!!! CTATCTTGTTTTTTCAGTCT+TGG + chr5.4:56539754-56539773 MS.gene26098:intron 30.0%
AAAACTCCAAACTAGTGCAA+AGG - chr5.4:56539397-56539416 None:intergenic 35.0%
AAAGGCCGATAATAGTTCAT+AGG - chr5.4:56539379-56539398 None:intergenic 35.0%
AGCTTGTAAATTTGCAGCAA+TGG + chr5.4:56538627-56538646 MS.gene26098:CDS 35.0%
AGGTTTGGATGATCGATAAT+TGG - chr5.4:56539139-56539158 None:intergenic 35.0%
ATCTGTTTGACACATGTACA+TGG + chr5.4:56539350-56539369 MS.gene26098:intron 35.0%
CGCTAGTTAACTAACTACAT+TGG + chr5.4:56538815-56538834 MS.gene26098:intron 35.0%
GCACATTAACACAGATTTCT+AGG - chr5.4:56539812-56539831 None:intergenic 35.0%
GTGCTTTACTTGTGAATAGT+GGG - chr5.4:56539541-56539560 None:intergenic 35.0%
GTGTTGTACCTGATACAATA+TGG - chr5.4:56539922-56539941 None:intergenic 35.0%
TATCGATCATCCAAACCTTT+AGG + chr5.4:56539141-56539160 MS.gene26098:intron 35.0%
TCTTGGAATCATGAAAGTCA+AGG + chr5.4:56540019-56540038 MS.gene26098:CDS 35.0%
! AAGATGAAAAGTGTATGGGA+AGG + chr5.4:56540092-56540111 MS.gene26098:CDS 35.0%
! CGTTATGTGATTAGAAGCAT+TGG + chr5.4:56538776-56538795 MS.gene26098:intron 35.0%
!! TGATTTTAGGTGATCCTTGA+GGG + chr5.4:56539101-56539120 MS.gene26098:intron 35.0%
AATATGTTGGAACCGTGAAG+AGG + chr5.4:56539892-56539911 MS.gene26098:CDS 40.0%
ACAAAGAGCTGAAGTTGACT+TGG + chr5.4:56539512-56539531 MS.gene26098:intron 40.0%
ACTACATTGGGAATTGGGAT+TGG + chr5.4:56538828-56538847 MS.gene26098:intron 40.0%
AGAGATAAGTGACAGAGAAG+TGG + chr5.4:56538909-56538928 MS.gene26098:intron 40.0%
AGTTTAATATAGCTGGGGAG+AGG - chr5.4:56539620-56539639 None:intergenic 40.0%
CAAGAACCTCTTATAAGGAC+CGG - chr5.4:56539423-56539442 None:intergenic 40.0%
CAATATGGTTCTCCTCTTCA+CGG - chr5.4:56539907-56539926 None:intergenic 40.0%
CGTGCTTTACTTGTGAATAG+TGG - chr5.4:56539542-56539561 None:intergenic 40.0%
CTACATTGGGAATTGGGATT+GGG + chr5.4:56538829-56538848 MS.gene26098:intron 40.0%
CTTCAAGAATCGGTAAGCAA+CGG - chr5.4:56539478-56539497 None:intergenic 40.0%
GACTCTTTGCCTTCAAGAAT+CGG - chr5.4:56539488-56539507 None:intergenic 40.0%
GAGGAGAACCATATTGTATC+AGG + chr5.4:56539911-56539930 MS.gene26098:CDS 40.0%
GTAAGCAACGGAAATTTCGT+CGG - chr5.4:56539466-56539485 None:intergenic 40.0%
GTCATGACTTGATATACCCA+AGG + chr5.4:56539980-56539999 MS.gene26098:intron 40.0%
GTTTAACAAAGAGGAGGGAT+TGG + chr5.4:56540055-56540074 MS.gene26098:CDS 40.0%
GTTTAATATAGCTGGGGAGA+GGG - chr5.4:56539619-56539638 None:intergenic 40.0%
TCAACTTCAGCTCTTTGTTC+AGG - chr5.4:56539510-56539529 None:intergenic 40.0%
TCACAAGTAAAGCACGTTCT+TGG + chr5.4:56539546-56539565 MS.gene26098:intron 40.0%
TTTAATATAGCTGGGGAGAG+GGG - chr5.4:56539618-56539637 None:intergenic 40.0%
!! ACTAGTTTGGAGTTTTCCTC+CGG + chr5.4:56539401-56539420 MS.gene26098:intron 40.0%
!! CTGATTTTAGGTGATCCTTG+AGG + chr5.4:56539100-56539119 MS.gene26098:intron 40.0%
AGTGACAGAGAAGTGGTAGT+TGG + chr5.4:56538916-56538935 MS.gene26098:intron 45.0%
ATTGGGATTGGGTGTCTTTG+TGG + chr5.4:56538840-56538859 MS.gene26098:intron 45.0%
GTGACAGAGAAGTGGTAGTT+GGG + chr5.4:56538917-56538936 MS.gene26098:intron 45.0%
TATCGGCCTTTGCACTAGTT+TGG + chr5.4:56539388-56539407 MS.gene26098:intron 45.0%
TCACGTGCAGGAATATCTTG+AGG + chr5.4:56539869-56539888 MS.gene26098:intron 45.0%
TCCTGATAGAGTTCTCTCCA+TGG + chr5.4:56538651-56538670 MS.gene26098:CDS 45.0%
TTCGTCGGTCAAACTGCAAA+AGG - chr5.4:56539451-56539470 None:intergenic 45.0%
TTTCCTCCGGTCCTTATAAG+AGG + chr5.4:56539414-56539433 MS.gene26098:intron 45.0%
!! CGTTGCTTACCGATTCTTGA+AGG + chr5.4:56539476-56539495 MS.gene26098:intron 45.0%
!! TTTTAGGTGATCCTTGAGGG+TGG + chr5.4:56539104-56539123 MS.gene26098:intron 45.0%
AAAGTCACGAATCGCGATCC+GGG + chr5.4:56538993-56539012 MS.gene26098:intron 50.0%
ACCACCTGTTGCATTGAGCA+AGG - chr5.4:56538677-56538696 None:intergenic 50.0%
CAAAGTCACGAATCGCGATC+CGG + chr5.4:56538992-56539011 MS.gene26098:intron 50.0%
GAACCTCTTATAAGGACCGG+AGG - chr5.4:56539420-56539439 None:intergenic 50.0%
GATCGCGATTCGTGACTTTG+TGG - chr5.4:56538992-56539011 None:intergenic 50.0%
GCCATGGAGAGAACTCTATC+AGG - chr5.4:56538655-56538674 None:intergenic 50.0%
GCGATTCGTGACTTTGTGGA+GGG - chr5.4:56538988-56539007 None:intergenic 50.0%
GGATTGGGTGTCTTTGTGGA+GGG + chr5.4:56538844-56538863 MS.gene26098:intron 50.0%
TGTTGCATTGAGCAAGGCCA+TGG - chr5.4:56538671-56538690 None:intergenic 50.0%
TTGCTCAATGCAACAGGTGG+TGG + chr5.4:56538676-56538695 MS.gene26098:CDS 50.0%
! ATGGCCTTGCTCAATGCAAC+AGG + chr5.4:56538670-56538689 MS.gene26098:CDS 50.0%
ATAGCTGGGGAGAGGGGTTA+GGG - chr5.4:56539612-56539631 None:intergenic 55.0%
CGCGATTCGTGACTTTGTGG+AGG - chr5.4:56538989-56539008 None:intergenic 55.0%
GCCTTGCTCAATGCAACAGG+TGG + chr5.4:56538673-56538692 MS.gene26098:CDS 55.0%
GGACTCAATTGCCACCCTCA+AGG - chr5.4:56539118-56539137 None:intergenic 55.0%
GGGATTGGGTGTCTTTGTGG+AGG + chr5.4:56538843-56538862 MS.gene26098:intron 55.0%
TATAGCTGGGGAGAGGGGTT+AGG - chr5.4:56539613-56539632 None:intergenic 55.0%
TGTGACTAGCAAGCACCACC+CGG - chr5.4:56539014-56539033 None:intergenic 55.0%
GTCACGAATCGCGATCCGGG+TGG + chr5.4:56538996-56539015 MS.gene26098:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 56538622 56540121 56538622 ID=MS.gene26098
chr5.4 mRNA 56538622 56540121 56538622 ID=MS.gene26098.t1;Parent=MS.gene26098
chr5.4 exon 56538622 56538717 56538622 ID=MS.gene26098.t1.exon1;Parent=MS.gene26098.t1
chr5.4 CDS 56538622 56538717 56538622 ID=cds.MS.gene26098.t1;Parent=MS.gene26098.t1
chr5.4 exon 56539879 56539932 56539879 ID=MS.gene26098.t1.exon2;Parent=MS.gene26098.t1
chr5.4 CDS 56539879 56539932 56539879 ID=cds.MS.gene26098.t1;Parent=MS.gene26098.t1
chr5.4 exon 56540002 56540121 56540002 ID=MS.gene26098.t1.exon3;Parent=MS.gene26098.t1
chr5.4 CDS 56540002 56540121 56540002 ID=cds.MS.gene26098.t1;Parent=MS.gene26098.t1
Gene Sequence

>MS.gene26098

ATGATAGCTTGTAAATTTGCAGCAATGGTTCCTGATAGAGTTCTCTCCATGGCCTTGCTCAATGCAACAGGTGGTGGTTTTCAATGTTTTCCAAAGGTACTATATTATGTGACCCTTGTATAAATCTGTGAAATGTTTCAATTTTTTTTTACGGCGTTATGTGATTAGAAGCATTGGTGATGCAACTTTGTAGCGCTAGTTAACTAACTACATTGGGAATTGGGATTGGGTGTCTTTGTGGAGGGAAATGTATGATGACTTTAGTGTATTTTACATTGTTTTATGAGAGAGATAAGTGACAGAGAAGTGGTAGTTGGGATTAGAGAGAGAGAGTAATATAGAAGAGAAAGTCACGGTGACTTTCCCTCCACAAAGTCACGAATCGCGATCCGGGTGGTGCTTGCTAGTCACACTTTTTAATTACAAACGTAGTTCAGGATAGTGAAGTTGCATACATAACAAATGGAATTTCTTACTACTGATTTTAGGTGATCCTTGAGGGTGGCAATTGAGTCCAATTATCGATCATCCAAACCTTTAGGAGAAATTTAGTTGAGAATTTATTTTTCTATGCTTTTGAGTGAGAATTTCGTAGTACTGAAATTTAGATGTTTGAGTGATTAAATGTATAAATAATATCTAGATTCAGCTGTTTGCAGTTTTTCCAATGCTTAATACAAATTTAATTGGAATGTATCTGCAATTAGATATATAATGTTGGAGTTATGATCTGTTTGACACATGTACATGGTTTCCTATGAACTATTATCGGCCTTTGCACTAGTTTGGAGTTTTCCTCCGGTCCTTATAAGAGGTTCTTGTTCTACCTTTTGCAGTTTGACCGACGAAATTTCCGTTGCTTACCGATTCTTGAAGGCAAAGAGTCCTGAACAAAGAGCTGAAGTTGACTTGGACACCCACTATTCACAAGTAAAGCACGTTCTTGGTTTTATTTATTATTTACAATCAACATATAAAAAATTGTCTCCCTAACCCCTCTCCCCAGCTATATTAAACTTACCATTAAAGTAGTGATTTTAAAAAATTGAATCACTCAAGATGTATTCGTATATGATATAAAAGAGTCTGGATTATCATTTTGTAATGAATATTAACATTAAGTTGAAGAAGACTATCTTGTTTTTTCAGTCTTGGGTCTACTCTGAAGTCTGAATTACTGTTAACAGCCTAGAAATCTGTGTTAATGTGCTATCTTTAGGATGAATATCAAAAACAAATTTTATATATCACGTGCAGGAATATCTTGAGGAATATGTTGGAACCGTGAAGAGGAGAACCATATTGTATCAGGTACAACACAAACTGAAAAGAAGCTTCAATTTAGGTTGTTGTCAATTGTCATGACTTGATATACCCAAGGTGAAAGATAAGAAAGATCTTGGAATCATGAAAGTCAAGGTCTTGTATTATCAGTTTAACAAAGAGGAGGGATTGGTTGTACTAGAAGAAAAGATGAAAAGTGTATGGGAAGGACCATAA

Protein sequence

>MS.gene26098.t1

MIACKFAAMVPDRVLSMALLNATGGGFQCFPKEYLEEYVGTVKRRTILYQVKDKKDLGIMKVKVLYYQFNKEEGLVVLEEKMKSVWEGP