AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28424

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28424.t1 MTR_8g093010 88.546 227 16 3 1 225 1 219 5.15e-142 395
MS.gene28424.t1 MTR_8g092840 86.222 225 30 1 1 224 1 225 1.49e-132 372
MS.gene28424.t1 MTR_8g092930 84.071 226 34 2 1 225 1 225 9.85e-128 360
MS.gene28424.t1 MTR_8g092970 80.531 226 34 2 1 225 1 217 4.36e-122 345
MS.gene28424.t1 MTR_8g092960 79.111 225 36 3 1 224 1 215 7.79e-119 337
MS.gene28424.t1 MTR_8g092990 83.260 227 36 2 1 225 1 227 6.19e-117 333
MS.gene28424.t1 MTR_8g092985 82.160 213 33 3 1 211 1 210 1.75e-113 323
MS.gene28424.t1 MTR_3g095560 63.090 233 72 4 1 225 1 227 3.70e-97 283
MS.gene28424.t1 MTR_3g094000 53.676 136 57 2 53 187 36 166 1.05e-42 142
MS.gene28424.t1 MTR_7g073200 46.835 158 73 4 45 198 18 168 1.22e-42 142
MS.gene28424.t1 MTR_5g024730 48.571 140 62 4 54 191 26 157 4.07e-37 129
MS.gene28424.t1 MTR_3g116000 46.575 146 65 4 58 198 26 163 7.06e-36 125
MS.gene28424.t1 MTR_8g073120 47.328 131 58 3 54 182 19 140 1.36e-34 122
MS.gene28424.t1 MTR_2g035800 38.710 155 75 4 33 183 7 145 2.14e-30 110
MS.gene28424.t1 MTR_4g094725 39.490 157 75 6 33 183 7 149 1.06e-29 108
MS.gene28424.t1 MTR_1g043410 27.922 154 95 3 39 183 20 166 6.07e-21 89.4
MS.gene28424.t1 MTR_7g117540 32.593 135 89 1 54 188 17 149 2.34e-20 87.4
MS.gene28424.t1 MTR_1g082550 33.557 149 88 5 42 186 32 173 3.27e-20 88.2
MS.gene28424.t1 MTR_7g113800 31.481 162 89 5 34 186 18 166 1.13e-19 86.3
MS.gene28424.t1 MTR_3g110190 46.154 78 41 1 111 188 64 140 1.81e-19 85.5
MS.gene28424.t1 MTR_1g100737 46.429 84 41 1 110 189 89 172 2.51e-19 82.8
MS.gene28424.t1 MTR_8g023400 45.570 79 40 2 113 190 92 168 8.22e-19 83.6
MS.gene28424.t1 MTR_6g087150 45.556 90 40 2 102 183 74 162 8.97e-19 84.0
MS.gene28424.t1 MTR_4g120270 43.056 72 38 1 111 182 78 146 3.42e-18 81.3
MS.gene28424.t1 MTR_4g063070 43.210 81 45 1 103 182 298 378 5.86e-18 82.0
MS.gene28424.t1 MTR_8g066780 35.333 150 91 4 57 201 55 203 7.32e-18 81.3
MS.gene28424.t1 MTR_6g055030 46.575 73 37 1 112 182 96 168 8.41e-18 80.9
MS.gene28424.t1 MTR_2g096350 43.678 87 44 2 135 221 130 211 1.00e-17 79.0
MS.gene28424.t1 MTR_3g099650 42.308 78 43 1 111 186 105 182 2.50e-17 80.1
MS.gene28424.t1 MTR_3g071300 32.331 133 84 3 56 185 22 151 3.43e-17 76.6
MS.gene28424.t1 MTR_5g026730 29.299 157 94 5 49 197 2 149 5.37e-17 75.9
MS.gene28424.t1 MTR_3g464780 43.590 78 41 1 112 186 101 178 7.43e-17 77.4
MS.gene28424.t1 MTR_5g071930 48.684 76 35 2 112 187 65 136 7.55e-17 76.3
MS.gene28424.t1 MTR_5g082940 44.737 76 41 1 111 185 73 148 8.20e-17 75.5
MS.gene28424.t1 MTR_7g076250 30.380 158 99 4 65 221 17 164 1.09e-16 75.5
MS.gene28424.t1 MTR_4g056590 42.308 78 40 2 112 189 99 171 1.61e-16 75.1
MS.gene28424.t1 MTR_5g064980 38.462 91 52 2 113 201 108 196 1.86e-16 77.4
MS.gene28424.t1 MTR_2g436100 38.095 84 51 1 99 182 79 161 2.55e-16 76.3
MS.gene28424.t1 MTR_4g129420 41.772 79 46 0 108 186 112 190 3.00e-16 77.4
MS.gene28424.t1 MTR_5g030010 31.206 141 85 4 57 186 55 194 4.25e-16 76.6
MS.gene28424.t1 MTR_1g492830 36.364 88 55 1 112 199 60 146 5.69e-16 74.3
MS.gene28424.t1 MTR_5g064990 31.159 138 89 4 52 186 55 189 6.28e-16 75.9
MS.gene28424.t1 MTR_7g116220 38.462 78 46 1 112 187 69 146 6.60e-16 73.2
MS.gene28424.t1 MTR_7g108855 38.298 94 48 3 114 198 67 159 8.45e-16 73.2
MS.gene28424.t1 MTR_5g066250 41.667 72 41 1 111 182 132 202 1.03e-15 75.5
MS.gene28424.t1 MTR_5g064890 31.034 116 77 2 73 186 86 200 1.74e-15 74.7
MS.gene28424.t1 MTR_2g055790 31.967 122 74 2 65 186 34 146 2.20e-15 72.0
MS.gene28424.t1 MTR_3g100400 41.667 72 41 1 111 182 128 198 2.36e-15 74.3
MS.gene28424.t1 MTR_4g093830 31.034 116 70 2 104 219 117 222 3.74e-15 73.6
MS.gene28424.t1 MTR_3g078450 38.043 92 56 1 95 185 58 149 4.61e-15 71.6
MS.gene28424.t1 MTR_3g114500 37.349 83 48 2 100 182 35 113 6.29e-15 69.3
MS.gene28424.t1 MTR_3g078470 36.735 98 60 2 89 185 53 149 6.58e-15 71.2
MS.gene28424.t1 MTR_8g089880 36.250 80 50 1 113 192 127 205 9.29e-15 71.6
MS.gene28424.t1 MTR_3g086700 33.871 124 73 3 61 182 1 117 9.62e-15 70.1
MS.gene28424.t1 MTR_5g034790 41.463 82 44 2 107 185 107 187 1.43e-14 72.0
MS.gene28424.t1 MTR_7g116180 40.506 79 43 2 110 185 64 141 1.99e-14 69.3
MS.gene28424.t1 MTR_2g090905 41.558 77 40 1 111 182 37 113 2.22e-14 67.8
MS.gene28424.t1 MTR_4g030070 33.333 87 56 1 104 190 76 160 2.43e-14 69.7
MS.gene28424.t1 MTR_8g074900 41.333 75 39 1 111 185 156 225 2.49e-14 69.7
MS.gene28424.t1 MTR_2g090950 39.744 78 42 1 110 182 88 165 2.50e-14 70.1
MS.gene28424.t1 MTR_4g108830 34.343 99 61 2 86 183 122 217 2.93e-14 69.7
MS.gene28424.t1 MTR_3g086730 40.000 80 46 1 108 185 41 120 2.99e-14 69.7
MS.gene28424.t1 MTR_1g056350 32.075 106 59 3 105 207 66 161 3.06e-14 68.6
MS.gene28424.t1 MTR_1g070425 40.506 79 43 2 110 185 65 142 3.15e-14 68.6
MS.gene28424.t1 MTR_1g070140 39.744 78 45 2 106 182 96 172 3.23e-14 70.9
MS.gene28424.t1 MTR_4g106680 35.354 99 57 2 109 201 102 199 3.41e-14 70.5
MS.gene28424.t1 MTR_6g007737 31.111 135 82 3 61 185 71 204 3.48e-14 70.9
MS.gene28424.t1 MTR_5g022920 38.961 77 46 1 110 185 158 234 3.50e-14 69.7
MS.gene28424.t1 MTR_2g009310 59.091 44 18 0 139 182 160 203 3.85e-14 70.9
MS.gene28424.t1 MTR_8g063660 39.286 84 47 2 107 187 59 141 4.24e-14 70.5
MS.gene28424.t1 MTR_6g007670 34.513 113 57 4 110 222 5 100 5.90e-14 69.3
MS.gene28424.t1 MTR_6g007670 47.222 72 36 2 112 182 144 214 2.04e-12 65.1
MS.gene28424.t1 MTR_5g076990 30.882 136 87 2 54 182 13 148 6.67e-14 69.7
MS.gene28424.t1 MTR_6g007680 41.096 73 42 1 110 182 37 108 7.69e-14 67.0
MS.gene28424.t1 MTR_1g074190 31.667 120 72 3 81 190 62 181 9.47e-14 69.7
MS.gene28424.t1 MTR_5g092790 41.463 82 45 2 103 182 147 227 9.93e-14 68.6
MS.gene28424.t1 MTR_3g012550 30.189 106 64 2 113 217 80 176 1.26e-13 67.0
MS.gene28424.t1 MTR_8g028465 33.981 103 52 3 113 215 90 176 1.43e-13 67.4
MS.gene28424.t1 MTR_3g091620 41.892 74 42 1 113 186 101 173 1.53e-13 68.9
MS.gene28424.t1 MTR_4g108830 34.021 97 60 2 88 183 96 189 1.59e-13 67.0
MS.gene28424.t1 MTR_7g079280 32.143 112 62 3 113 223 80 178 1.62e-13 66.6
MS.gene28424.t1 MTR_2g041200 50.909 55 27 0 128 182 97 151 2.16e-13 66.2
MS.gene28424.t1 MTR_7g059360 45.455 66 35 1 119 183 136 201 2.83e-13 67.0
MS.gene28424.t1 MTR_3g091200 39.437 71 42 1 113 182 303 373 3.33e-13 68.2
MS.gene28424.t1 MTR_8g099495 40.278 72 42 1 113 183 319 390 3.87e-13 68.2
MS.gene28424.t1 MTR_5g026720 53.488 43 20 0 140 182 93 135 4.79e-13 65.1
MS.gene28424.t1 MTR_8g066740 30.709 127 81 2 63 182 22 148 5.36e-13 67.0
MS.gene28424.t1 MTR_8g032690 50.000 52 26 0 140 191 19 70 5.57e-13 63.5
MS.gene28424.t1 MTR_5g009190 40.278 72 42 1 113 183 266 337 7.39e-13 67.0
MS.gene28424.t1 MTR_5g009210 40.845 71 41 1 113 182 287 357 8.51e-13 67.0
MS.gene28424.t1 MTR_7g117350 28.000 125 81 2 68 183 241 365 8.98e-13 67.0
MS.gene28424.t1 MTR_1g026070 36.111 72 42 1 111 182 46 113 9.43e-13 63.5
MS.gene28424.t1 MTR_7g117350 28.000 125 81 2 68 183 233 357 9.93e-13 66.6
MS.gene28424.t1 MTR_5g092750 62.791 43 16 0 140 182 185 227 1.11e-12 65.5
MS.gene28424.t1 MTR_5g009200 40.845 71 41 1 113 182 322 392 1.55e-12 66.2
MS.gene28424.t1 MTR_3g087900 47.692 65 29 3 123 186 55 115 1.64e-12 63.5
MS.gene28424.t1 MTR_2g079320 31.132 106 68 1 85 185 99 204 2.49e-12 63.9
MS.gene28424.t1 MTR_4g022410 30.657 137 88 3 63 199 43 172 2.62e-12 63.5
MS.gene28424.t1 MTR_8g071815 51.111 45 22 0 138 182 88 132 2.64e-12 63.5
MS.gene28424.t1 MTR_7g079290 30.952 84 55 1 112 192 124 207 3.83e-12 63.5
MS.gene28424.t1 MTR_3g078120 30.579 121 81 2 63 182 9 127 6.36e-12 61.6
MS.gene28424.t1 MTR_8g019710 59.091 44 18 0 139 182 53 96 6.97e-12 61.2
MS.gene28424.t1 MTR_7g116200 36.842 76 46 1 107 182 62 135 8.51e-12 62.0
MS.gene28424.t1 MTR_8g099515 41.667 72 39 2 113 182 309 379 1.03e-11 63.9
MS.gene28424.t1 MTR_3g078540 42.857 63 35 1 127 188 66 128 1.47e-11 60.8
MS.gene28424.t1 MTR_3g081030 35.802 81 50 1 119 199 100 178 2.07e-11 61.6
MS.gene28424.t1 MTR_4g116460 53.333 45 19 2 139 182 440 483 2.18e-11 63.2
MS.gene28424.t1 MTR_4g116460 53.333 45 19 2 139 182 483 526 2.19e-11 63.2
MS.gene28424.t1 MTR_6g055030 53.659 41 19 0 142 182 97 137 5.83e-11 61.2
MS.gene28424.t1 MTR_8g027745 36.620 71 44 1 113 182 286 356 6.01e-11 61.6
MS.gene28424.t1 MTR_1g090480 27.778 126 77 2 94 216 53 167 6.34e-11 59.3
MS.gene28424.t1 MTR_3g078520 31.461 89 61 0 94 182 57 145 7.02e-11 59.3
MS.gene28424.t1 MTR_8g027980 33.333 75 48 1 116 188 93 167 9.31e-11 60.5
MS.gene28424.t1 MTR_4g106960 59.091 44 17 1 139 182 177 219 9.76e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28424.t1 AT1G49230 49.321 221 86 5 11 223 17 219 2.93e-65 201
MS.gene28424.t1 AT1G49210 47.887 213 90 4 21 224 23 223 7.87e-62 193
MS.gene28424.t1 AT1G49200 45.540 213 95 4 21 224 24 224 9.24e-62 192
MS.gene28424.t1 AT1G49220 49.754 203 81 5 21 214 23 213 1.37e-61 193
MS.gene28424.t1 AT3G18773 44.196 224 107 5 6 224 8 218 6.68e-57 180
MS.gene28424.t1 AT5G05280 54.198 131 53 2 57 187 37 160 6.33e-45 148
MS.gene28424.t1 AT3G10910 51.880 133 60 2 57 186 29 160 2.60e-42 142
MS.gene28424.t1 AT1G76410 44.595 148 79 2 41 188 8 152 6.36e-39 133
MS.gene28424.t1 AT1G20823 41.341 179 77 4 11 186 4 157 5.08e-37 129
MS.gene28424.t1 AT5G01880 42.246 187 78 7 12 196 1 159 2.39e-31 113
MS.gene28424.t1 AT5G47610 36.810 163 85 2 23 182 2 149 1.58e-30 111
MS.gene28424.t1 AT2G17450 41.045 134 62 3 58 185 25 147 2.97e-30 110
MS.gene28424.t1 AT4G35480 36.571 175 83 6 58 216 23 185 1.02e-27 104
MS.gene28424.t1 AT4G17245 38.346 133 67 2 51 182 27 145 1.79e-25 98.2
MS.gene28424.t1 AT1G72200 32.934 167 95 7 45 201 47 206 7.19e-20 87.4
MS.gene28424.t1 AT4G40070 30.303 198 114 6 24 214 20 200 1.16e-19 85.9
MS.gene28424.t1 AT1G72310 48.193 83 40 2 105 186 93 173 2.86e-19 85.1
MS.gene28424.t1 AT2G35000 34.091 132 80 4 61 186 51 181 3.22e-19 85.5
MS.gene28424.t1 AT3G05200 36.567 134 76 4 59 187 46 175 5.42e-19 84.7
MS.gene28424.t1 AT4G09110 34.711 121 71 3 64 182 51 165 2.09e-18 82.4
MS.gene28424.t1 AT2G25410 45.977 87 45 1 98 182 283 369 2.32e-18 82.8
MS.gene28424.t1 AT4G09120 34.677 124 73 3 64 185 51 168 3.00e-18 82.4
MS.gene28424.t1 AT3G16720 36.719 128 67 6 91 212 76 195 4.84e-18 81.3
MS.gene28424.t1 AT2G20030 48.571 70 35 1 113 182 98 166 6.37e-18 81.6
MS.gene28424.t1 AT1G35330 31.293 147 92 4 64 202 46 191 6.77e-18 81.3
MS.gene28424.t1 AT1G53820 43.590 78 40 2 109 185 91 165 9.82e-18 80.5
MS.gene28424.t1 AT3G14320 47.887 71 35 1 113 183 63 131 1.08e-17 78.6
MS.gene28424.t1 AT2G46494 30.657 137 89 2 52 182 222 358 1.74e-17 80.5
MS.gene28424.t1 AT2G46493 39.080 87 52 1 97 182 94 180 2.01e-17 77.4
MS.gene28424.t1 AT5G27420 38.776 98 54 2 96 187 74 171 4.88e-17 79.3
MS.gene28424.t1 AT1G28040 38.384 99 58 2 87 182 197 295 5.58e-17 78.2
MS.gene28424.t1 AT2G27940 39.773 88 50 2 111 198 113 197 5.81e-17 77.4
MS.gene28424.t1 AT4G28890 45.070 71 38 1 113 183 97 166 6.26e-17 79.3
MS.gene28424.t1 AT2G18650 44.578 83 43 1 107 186 90 172 7.75e-17 79.0
MS.gene28424.t1 AT4G30400 41.333 75 44 0 108 182 102 176 1.34e-16 78.2
MS.gene28424.t1 AT1G22500 30.872 149 97 3 53 198 31 176 1.49e-16 77.8
MS.gene28424.t1 AT4G15975 37.383 107 64 2 113 219 51 154 1.86e-16 75.9
MS.gene28424.t1 AT1G04360 30.303 132 82 2 61 182 45 176 2.50e-16 77.4
MS.gene28424.t1 AT3G19140 37.500 104 61 2 80 183 23 122 5.42e-16 72.4
MS.gene28424.t1 AT1G74410 37.363 91 56 1 95 185 132 221 5.80e-16 74.3
MS.gene28424.t1 AT1G23980 33.728 169 88 8 38 186 26 190 7.06e-16 75.9
MS.gene28424.t1 AT3G03550 60.417 48 19 0 139 186 158 205 7.39e-16 75.9
MS.gene28424.t1 AT4G09130 43.421 76 41 1 112 185 90 165 7.80e-16 75.5
MS.gene28424.t1 AT4G17905 53.333 60 23 1 139 198 154 208 8.14e-16 75.1
MS.gene28424.t1 AT4G17905 53.333 60 23 1 139 198 180 234 8.30e-16 75.5
MS.gene28424.t1 AT5G05810 43.056 72 40 1 111 182 118 188 9.63e-16 75.9
MS.gene28424.t1 AT4G09100 39.241 79 46 2 106 182 49 127 1.10e-15 71.2
MS.gene28424.t1 AT5G17600 40.659 91 51 2 93 182 96 184 1.31e-15 75.1
MS.gene28424.t1 AT5G57750 40.000 75 45 0 109 183 91 165 1.37e-15 73.2
MS.gene28424.t1 AT1G32361 34.351 131 75 5 94 218 108 233 2.35e-15 73.9
MS.gene28424.t1 AT2G34990 29.921 127 87 1 58 182 34 160 2.84e-15 73.9
MS.gene28424.t1 AT2G34990 29.921 127 87 1 58 182 12 138 2.86e-15 73.6
MS.gene28424.t1 AT5G66070 44.000 75 39 2 111 185 150 221 6.69e-15 71.2
MS.gene28424.t1 AT5G66070 44.000 75 39 2 111 185 150 221 6.69e-15 71.2
MS.gene28424.t1 AT4G17920 31.061 132 84 3 61 185 24 155 6.89e-15 72.4
MS.gene28424.t1 AT3G48030 38.043 92 47 3 101 185 86 174 7.04e-15 72.0
MS.gene28424.t1 AT5G42200 36.250 80 50 1 103 182 68 146 9.22e-15 69.7
MS.gene28424.t1 AT3G18930 35.849 106 57 4 113 209 120 223 9.29e-15 72.8
MS.gene28424.t1 AT3G18930 35.849 106 57 4 113 209 120 223 9.29e-15 72.8
MS.gene28424.t1 AT3G18930 35.849 106 57 4 113 209 120 223 9.29e-15 72.8
MS.gene28424.t1 AT5G66070 44.000 75 39 2 111 185 174 245 1.09e-14 71.2
MS.gene28424.t1 AT3G62690 30.168 179 104 6 44 214 16 181 1.09e-14 71.2
MS.gene28424.t1 AT3G48030 38.043 92 47 3 101 185 164 252 1.23e-14 72.0
MS.gene28424.t1 AT2G46495 39.506 81 47 1 104 182 282 362 2.18e-14 71.6
MS.gene28424.t1 AT2G46495 39.506 81 47 1 104 182 254 334 2.48e-14 71.2
MS.gene28424.t1 AT4G10160 33.071 127 75 4 63 186 24 143 3.13e-14 69.7
MS.gene28424.t1 AT2G35910 35.789 95 51 2 113 207 121 205 3.36e-14 69.3
MS.gene28424.t1 AT5G06490 34.653 101 51 2 113 207 96 187 3.47e-14 68.9
MS.gene28424.t1 AT5G10380 37.975 79 48 1 108 186 104 181 4.58e-14 70.1
MS.gene28424.t1 AT1G72220 56.818 44 19 0 139 182 176 219 6.86e-14 70.5
MS.gene28424.t1 AT2G35420 41.667 72 41 1 111 182 75 145 1.07e-13 68.6
MS.gene28424.t1 AT5G58580 36.957 92 56 1 95 186 95 184 1.29e-13 68.9
MS.gene28424.t1 AT5G53110 39.437 71 42 1 113 182 302 372 1.81e-13 68.9
MS.gene28424.t1 AT2G46160 32.353 102 57 2 113 207 104 200 2.11e-13 67.0
MS.gene28424.t1 AT2G46160 32.353 102 57 2 113 207 104 200 2.11e-13 67.0
MS.gene28424.t1 AT4G33565 52.000 50 24 0 139 188 216 265 2.17e-13 68.6
MS.gene28424.t1 AT3G11110 36.957 92 56 1 93 182 58 149 3.77e-13 65.5
MS.gene28424.t1 AT2G34000 37.333 75 44 1 112 183 60 134 5.52e-13 64.7
MS.gene28424.t1 AT4G10150 28.966 145 93 4 46 186 19 157 6.31e-13 66.2
MS.gene28424.t1 AT5G40250 48.980 49 25 0 138 186 141 189 6.89e-13 67.4
MS.gene28424.t1 AT5G07040 34.722 72 46 1 113 183 66 137 7.26e-13 64.7
MS.gene28424.t1 AT4G35840 35.556 90 56 1 96 183 145 234 8.60e-13 65.9
MS.gene28424.t1 AT2G42360 36.000 75 48 0 108 182 76 150 1.04e-12 65.5
MS.gene28424.t1 AT5G36001 59.574 47 19 0 137 183 261 307 1.15e-12 66.2
MS.gene28424.t1 AT2G47560 41.096 73 42 1 111 182 78 150 1.24e-12 65.1
MS.gene28424.t1 AT2G17730 56.818 44 19 0 140 183 196 239 1.80e-12 65.1
MS.gene28424.t1 AT2G18670 34.667 75 49 0 108 182 78 152 1.84e-12 63.9
MS.gene28424.t1 AT5G43420 56.818 44 19 0 139 182 137 180 2.98e-12 65.5
MS.gene28424.t1 AT5G46650 27.407 135 88 3 61 185 26 160 3.22e-12 64.7
MS.gene28424.t1 AT1G33480 33.784 74 47 1 113 186 75 146 3.32e-12 64.7
MS.gene28424.t1 AT4G26400 44.118 68 30 2 115 182 223 282 4.84e-12 64.7
MS.gene28424.t1 AT4G26400 44.118 68 30 2 115 182 223 282 4.84e-12 64.7
MS.gene28424.t1 AT1G35630 42.254 71 39 2 114 183 207 276 5.16e-12 64.3
MS.gene28424.t1 AT3G61550 28.800 125 70 3 106 220 92 207 7.90e-12 62.8
MS.gene28424.t1 AT5G56340 39.024 82 41 3 101 182 228 300 9.93e-12 63.9
MS.gene28424.t1 AT1G53010 50.000 50 25 0 139 188 129 178 1.54e-11 61.2
MS.gene28424.t1 AT2G42350 36.000 75 46 1 108 182 70 142 2.79e-11 61.2
MS.gene28424.t1 AT4G11360 37.634 93 53 3 96 186 44 133 2.84e-11 60.1
MS.gene28424.t1 AT3G20395 36.264 91 54 2 96 185 129 216 3.63e-11 61.2
MS.gene28424.t1 AT2G03000 51.064 47 22 1 139 185 480 525 6.89e-11 61.6
MS.gene28424.t1 AT3G47990 59.091 44 17 1 139 182 234 276 7.87e-11 61.2
MS.gene28424.t1 AT2G37580 52.273 44 21 0 139 182 141 184 8.36e-11 60.1
MS.gene28424.t1 AT3G47990 59.091 44 17 1 139 182 218 260 8.42e-11 61.2
MS.gene28424.t1 AT3G47990 59.091 44 17 1 139 182 177 219 8.52e-11 60.8
MS.gene28424.t1 AT3G47990 59.091 44 17 1 139 182 177 219 8.52e-11 60.8
MS.gene28424.t1 AT3G47990 59.091 44 17 1 139 182 177 219 8.52e-11 60.8
MS.gene28424.t1 AT1G22670 34.722 72 45 2 113 183 205 275 9.74e-11 61.2

Find 44 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCATAAGGTGTGGTTTA+AGG 0.114506 4.4:-8809891 MS.gene28424:CDS
TTTCTTCTTGATTCCTTTGT+TGG 0.230023 4.4:+8809801 None:intergenic
TTATGGTTGTTTGTTAAAAC+AGG 0.262480 4.4:+8810015 None:intergenic
CGAAGAAGAGTCGTTGTTTA+GGG 0.283250 4.4:+8809855 None:intergenic
TCGAAGAAGAGTCGTTGTTT+AGG 0.298317 4.4:+8809854 None:intergenic
TGAAATCCATGGTTGCATTT+AGG 0.344720 4.4:+8809661 None:intergenic
TGAGTTGAAACTACCAAGTT+TGG 0.353182 4.4:-8809743 MS.gene28424:CDS
AATATGAGTCTGTTGAATTA+TGG 0.355133 4.4:+8809998 None:intergenic
TCCAAAGCTTCATGCTCTAA+TGG 0.359601 4.4:+8809496 None:intergenic
GTTAGTGATTGATGATCAAC+TGG 0.362794 4.4:+8810045 None:intergenic
AATTCAACAGACTCATATTT+CGG 0.363143 4.4:-8809994 MS.gene28424:CDS
TCTAGCACTGGCACAACAAT+TGG 0.383583 4.4:+8809538 None:intergenic
CAGCAACCCTTCACAACAAT+TGG 0.401223 4.4:-8809824 MS.gene28424:CDS
ACCATTAGAGCATGAAGCTT+TGG 0.405256 4.4:-8809497 MS.gene28424:CDS
CAGTGCTAGAAACCGTTATA+AGG 0.412848 4.4:-8809525 MS.gene28424:CDS
ATGTTCGTTGCATTGACAAA+TGG 0.437642 4.4:-8809639 MS.gene28424:CDS
TAATGGTTGGATCCTTATAA+CGG 0.468398 4.4:+8809513 None:intergenic
CTTATAACGGTTTCTAGCAC+TGG 0.474285 4.4:+8809526 None:intergenic
AGAGTTCACAAAGGGTGAAA+AGG 0.489010 4.4:-8809695 MS.gene28424:CDS
TTGCATTGACAAATGGCTAA+AGG 0.492896 4.4:-8809632 MS.gene28424:CDS
AGCATATAAGAGCACACAAT+AGG 0.493530 4.4:+8809929 None:intergenic
TTGCATTAAACTCCATCATA+AGG 0.493755 4.4:-8809903 MS.gene28424:CDS
CCTTAAACCACACCTTATGA+TGG 0.504302 4.4:+8809891 None:intergenic
AAGCTTCATGCTCTAATGGT+TGG 0.509803 4.4:+8809500 None:intergenic
TTGTGGAATCTCACACAAGA+AGG 0.511006 4.4:-8810083 MS.gene28424:CDS
TTGTTGGCCAATTGTTGTGA+AGG 0.519082 4.4:+8809817 None:intergenic
ATGGAGAAGTGGTGAAGTAA+AGG 0.523047 4.4:+8810113 None:intergenic
ACACACTCTATATCCAAACT+TGG 0.536274 4.4:+8809730 None:intergenic
ACTTCTCCATGAGCTTCTTG+TGG 0.539026 4.4:-8810100 MS.gene28424:CDS
TGTTGGCCAATTGTTGTGAA+GGG 0.559010 4.4:+8809818 None:intergenic
TCAGCTGAATAGCTCACTGT+GGG 0.566806 4.4:+8809763 None:intergenic
TATGGTTGTTTGTTAAAACA+GGG 0.577409 4.4:+8810016 None:intergenic
GAGATTCCACAAGAAGCTCA+TGG 0.584576 4.4:+8810094 None:intergenic
TGTCTCTCAGAGTTCACAAA+GGG 0.586259 4.4:-8809703 MS.gene28424:CDS
ATGGTTGTTTGTTAAAACAG+GGG 0.588201 4.4:+8810017 None:intergenic
AGAAGGCACTGTCTACACTT+AGG 0.588478 4.4:+8809595 None:intergenic
ACAAGAAGCTCATGGAGAAG+TGG 0.600282 4.4:+8810102 None:intergenic
TCACAACAATTGGCCAACAA+AGG 0.611058 4.4:-8809814 MS.gene28424:CDS
TTAAACTCCATCATAAGGTG+TGG 0.627123 4.4:-8809898 MS.gene28424:CDS
TGCAACGAACATGAAATCCA+TGG 0.629921 4.4:+8809650 None:intergenic
CTCAGCTGAATAGCTCACTG+TGG 0.665359 4.4:+8809762 None:intergenic
ATGTCTCTCAGAGTTCACAA+AGG 0.686490 4.4:-8809704 MS.gene28424:CDS
CAGCTGAATAGCTCACTGTG+GGG 0.730132 4.4:+8809764 None:intergenic
TTAGTGATTGATGATCAACT+GGG 0.748243 4.4:+8810046 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTGTAGAAAAATTGTT+GGG - chr4.4:8810033-8810052 MS.gene28424:CDS 20.0%
! AATATGAGTCTGTTGAATTA+TGG + chr4.4:8809608-8809627 None:intergenic 25.0%
! AATTCAACAGACTCATATTT+CGG - chr4.4:8809609-8809628 MS.gene28424:CDS 25.0%
! GAAACTTGTAGAAAAATTGT+TGG - chr4.4:8810032-8810051 MS.gene28424:CDS 25.0%
!! TATGGTTGTTTGTTAAAACA+GGG + chr4.4:8809590-8809609 None:intergenic 25.0%
!! TTATGGTTGTTTGTTAAAAC+AGG + chr4.4:8809591-8809610 None:intergenic 25.0%
!! TTTTTCTACAAGTTTCAAGA+AGG + chr4.4:8810028-8810047 None:intergenic 25.0%
TAATGGTTGGATCCTTATAA+CGG + chr4.4:8810093-8810112 None:intergenic 30.0%
TTGCATTAAACTCCATCATA+AGG - chr4.4:8809700-8809719 MS.gene28424:CDS 30.0%
TTTCTTCTTGATTCCTTTGT+TGG + chr4.4:8809805-8809824 None:intergenic 30.0%
! ATGGTTGTTTGTTAAAACAG+GGG + chr4.4:8809589-8809608 None:intergenic 30.0%
! TTAGTGATTGATGATCAACT+GGG + chr4.4:8809560-8809579 None:intergenic 30.0%
ACACACTCTATATCCAAACT+TGG + chr4.4:8809876-8809895 None:intergenic 35.0%
TGAAATCCATGGTTGCATTT+AGG + chr4.4:8809945-8809964 None:intergenic 35.0%
TTAAACTCCATCATAAGGTG+TGG - chr4.4:8809705-8809724 MS.gene28424:CDS 35.0%
! AGCATATAAGAGCACACAAT+AGG + chr4.4:8809677-8809696 None:intergenic 35.0%
! ATGTTCGTTGCATTGACAAA+TGG - chr4.4:8809964-8809983 MS.gene28424:CDS 35.0%
! ATTTTGCCTAAATGCAACCA+TGG - chr4.4:8809936-8809955 MS.gene28424:CDS 35.0%
! GTTAGTGATTGATGATCAAC+TGG + chr4.4:8809561-8809580 None:intergenic 35.0%
! TGAGTTGAAACTACCAAGTT+TGG - chr4.4:8809860-8809879 MS.gene28424:CDS 35.0%
! TTGCATTGACAAATGGCTAA+AGG - chr4.4:8809971-8809990 MS.gene28424:CDS 35.0%
AAGCTTCATGCTCTAATGGT+TGG + chr4.4:8810106-8810125 None:intergenic 40.0%
ACCATTAGAGCATGAAGCTT+TGG - chr4.4:8810106-8810125 MS.gene28424:CDS 40.0%
AGAGTTCACAAAGGGTGAAA+AGG - chr4.4:8809908-8809927 MS.gene28424:CDS 40.0%
ATGGAGAAGTGGTGAAGTAA+AGG + chr4.4:8809493-8809512 None:intergenic 40.0%
ATGTCTCTCAGAGTTCACAA+AGG - chr4.4:8809899-8809918 MS.gene28424:CDS 40.0%
CAGTGCTAGAAACCGTTATA+AGG - chr4.4:8810078-8810097 MS.gene28424:CDS 40.0%
CCATCATAAGGTGTGGTTTA+AGG - chr4.4:8809712-8809731 MS.gene28424:CDS 40.0%
CCTTAAACCACACCTTATGA+TGG + chr4.4:8809715-8809734 None:intergenic 40.0%
CGAAGAAGAGTCGTTGTTTA+GGG + chr4.4:8809751-8809770 None:intergenic 40.0%
TCACAACAATTGGCCAACAA+AGG - chr4.4:8809789-8809808 MS.gene28424:CDS 40.0%
TCCAAAGCTTCATGCTCTAA+TGG + chr4.4:8810110-8810129 None:intergenic 40.0%
TCGAAGAAGAGTCGTTGTTT+AGG + chr4.4:8809752-8809771 None:intergenic 40.0%
TGCAACGAACATGAAATCCA+TGG + chr4.4:8809956-8809975 None:intergenic 40.0%
TGTCTCTCAGAGTTCACAAA+GGG - chr4.4:8809900-8809919 MS.gene28424:CDS 40.0%
TGTTGGCCAATTGTTGTGAA+GGG + chr4.4:8809788-8809807 None:intergenic 40.0%
TTGTGGAATCTCACACAAGA+AGG - chr4.4:8809520-8809539 MS.gene28424:CDS 40.0%
TTGTTGGCCAATTGTTGTGA+AGG + chr4.4:8809789-8809808 None:intergenic 40.0%
!! CTTATAACGGTTTCTAGCAC+TGG + chr4.4:8810080-8810099 None:intergenic 40.0%
ACAAGAAGCTCATGGAGAAG+TGG + chr4.4:8809504-8809523 None:intergenic 45.0%
ACTTCTCCATGAGCTTCTTG+TGG - chr4.4:8809503-8809522 MS.gene28424:CDS 45.0%
CAGCAACCCTTCACAACAAT+TGG - chr4.4:8809779-8809798 MS.gene28424:CDS 45.0%
GAGATTCCACAAGAAGCTCA+TGG + chr4.4:8809512-8809531 None:intergenic 45.0%
TCAGCTGAATAGCTCACTGT+GGG + chr4.4:8809843-8809862 None:intergenic 45.0%
!! AGAAGGCACTGTCTACACTT+AGG + chr4.4:8810011-8810030 None:intergenic 45.0%
!! TCTAGCACTGGCACAACAAT+TGG + chr4.4:8810068-8810087 None:intergenic 45.0%
CAGCTGAATAGCTCACTGTG+GGG + chr4.4:8809842-8809861 None:intergenic 50.0%
CTCAGCTGAATAGCTCACTG+TGG + chr4.4:8809844-8809863 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 8809474 8810151 8809474 ID=MS.gene28424
chr4.4 mRNA 8809474 8810151 8809474 ID=MS.gene28424.t1;Parent=MS.gene28424
chr4.4 exon 8809474 8810151 8809474 ID=MS.gene28424.t1.exon1;Parent=MS.gene28424.t1
chr4.4 CDS 8809474 8810151 8809474 ID=cds.MS.gene28424.t1;Parent=MS.gene28424.t1
Gene Sequence

>MS.gene28424

ATGTATGCTTCTAGTTCCTTTACTTCACCACTTCTCCATGAGCTTCTTGTGGAATCTCACACAAGAAGGTTACTCATTCAAAACCCAGTTGATCATCAATCACTAACAAACTCCCCTGTTTTAACAAACAACCATAATTCAACAGACTCATATTTCGGAGCTCGTGAATTTGATTCAAATGTTATAATGATACTTGCAGTCCTATTGTGTGCTCTTATATGCTCACTTGCATTAAACTCCATCATAAGGTGTGGTTTAAGGTTTTCAAACGTAGCCCTAAACAACGACTCTTCTTCGAGTTCGAGCAGCAACCCTTCACAACAATTGGCCAACAAAGGAATCAAGAAGAAAGCTCTCAAAACATTCCCCACAGTGAGCTATTCAGCTGAGTTGAAACTACCAAGTTTGGATATAGAGTGTGTGATATGTCTCTCAGAGTTCACAAAGGGTGAAAAGGTGCGCATTTTGCCTAAATGCAACCATGGATTTCATGTTCGTTGCATTGACAAATGGCTAAAGGAACACTCATCATGTCCTAAGTGTAGACAGTGCCTTCTTGAAACTTGTAGAAAAATTGTTGGGTCACAACAACCAATTGTTGTGCCAGTGCTAGAAACCGTTATAAGGATCCAACCATTAGAGCATGAAGCTTTGGAACGTAACTACAGAGAAATATAA
Protein sequence

>MS.gene28424.t1

MYASSSFTSPLLHELLVESHTRRLLIQNPVDHQSLTNSPVLTNNHNSTDSYFGAREFDSNVIMILAVLLCALICSLALNSIIRCGLRFSNVALNNDSSSSSSSNPSQQLANKGIKKKALKTFPTVSYSAELKLPSLDIECVICLSEFTKGEKVRILPKCNHGFHVRCIDKWLKEHSSCPKCRQCLLETCRKIVGSQQPIVVPVLETVIRIQPLEHEALERNYREI