AlfalfaGEDB | MS.gene70249

M. sativa cultivar XinJiangDaYe / MS.gene70249

KEGG: K19040
GO: -
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70249.t1	XP_003630211.1	61.9	223	16	2	4	165	5	219	2.00E-63	251.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70249.t1	Q6NML0	57.1	119	47	1	50	164	105	223	1.1e-36	154.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70249.t1	G7LD48	61.9	223	16	2	4	165	5	219	1.5e-63	251.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene70249	MS.gene030657	PPI
MS.gene58111	MS.gene70249	PPI
MS.gene28426	MS.gene70249	PPI
MS.gene70249	MS.gene030659	PPI
MS.gene70249	MS.gene94889	PPI
MS.gene70253	MS.gene70249	PPI
MS.gene70249	MS.gene28429	PPI
MS.gene70249	MS.gene58106	PPI
MS.gene70249	MS.gene013346	PPI
MS.gene70249	MS.gene28425	PPI
MS.gene70249	MS.gene88015	PPI
MS.gene70249	MS.gene70250	PPI
MS.gene70249	MS.gene48772	PPI
MS.gene049319	MS.gene70249	PPI
MS.gene70249	MS.gene030651	PPI
MS.gene70249	MS.gene76770	PPI
MS.gene06444	MS.gene70249	PPI
MS.gene58108	MS.gene70249	PPI
MS.gene70249	MS.gene28430	PPI
MS.gene70249	MS.gene58108	PPI
MS.gene70249	MS.gene58109	PPI
MS.gene70249	MS.gene030655	PPI
MS.gene70249	MS.gene31002	PPI
MS.gene70249	MS.gene030652	PPI
MS.gene70249	MS.gene70254	PPI
MS.gene70249	MS.gene28428	PPI
MS.gene70249	MS.gene030660	PPI
MS.gene030658	MS.gene70249	PPI
MS.gene70249	MS.gene28427	PPI
MS.gene70249	MS.gene030654	PPI
MS.gene70249	MS.gene70255	PPI
MS.gene70249	MS.gene030653	PPI
MS.gene58109	MS.gene70249	PPI
MS.gene70249	MS.gene58107	PPI
MS.gene70249	MS.gene70253	PPI
MS.gene70249	MS.gene58110	PPI
MS.gene70249	MS.gene28431	PPI
MS.gene70249	MS.gene030656	PPI
MS.gene70249	MS.gene58104	PPI
MS.gene70256	MS.gene70249	PPI
MS.gene70249	MS.gene06444	PPI
MS.gene70249	MS.gene70257	PPI
MS.gene70249	MS.gene58105	PPI
MS.gene70249	MS.gene28424	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70249.t1	MTR_8g093010	88.811	143	8	1	23	165	85	219	2.22e-89	260
MS.gene70249.t1	MTR_8g092840	86.713	143	18	1	23	164	83	225	7.16e-79	234
MS.gene70249.t1	MTR_8g092960	59.447	217	33	3	1	164	1	215	1.46e-78	233
MS.gene70249.t1	MTR_8g092930	84.028	144	21	2	23	165	83	225	4.12e-76	226
MS.gene70249.t1	MTR_8g092970	78.472	144	21	2	23	165	83	217	1.63e-70	212
MS.gene70249.t1	MTR_8g092990	77.019	161	31	2	6	165	72	227	4.87e-69	209
MS.gene70249.t1	MTR_8g092985	54.762	210	36	3	1	151	1	210	6.04e-69	208
MS.gene70249.t1	MTR_3g095560	66.443	149	42	2	23	165	81	227	2.85e-66	202
MS.gene70249.t1	MTR_3g094000	52.830	106	44	2	23	127	66	166	4.17e-30	108
MS.gene70249.t1	MTR_5g024730	54.737	95	40	3	40	131	63	157	1.39e-28	105
MS.gene70249.t1	MTR_3g116000	48.571	105	47	3	39	138	61	163	1.27e-26	99.8
MS.gene70249.t1	MTR_7g073200	45.833	120	54	4	23	138	56	168	1.67e-26	99.0
MS.gene70249.t1	MTR_8g073120	50.588	85	37	2	41	122	58	140	4.55e-26	98.6
MS.gene70249.t1	MTR_2g035800	53.425	73	33	1	51	123	74	145	3.35e-23	90.1
MS.gene70249.t1	MTR_4g094725	56.757	74	31	1	51	123	76	149	5.45e-23	89.4
MS.gene70249.t1	MTR_1g100737	41.667	96	52	1	38	129	77	172	9.63e-20	82.0
MS.gene70249.t1	MTR_8g023400	45.570	79	40	2	53	130	92	168	1.54e-19	83.6
MS.gene70249.t1	MTR_3g110190	46.154	78	41	1	51	128	64	140	1.87e-19	83.6
MS.gene70249.t1	MTR_7g117540	46.667	75	38	1	54	128	77	149	8.24e-19	81.3
MS.gene70249.t1	MTR_1g082550	37.391	115	54	3	20	126	69	173	9.19e-19	82.0
MS.gene70249.t1	MTR_5g082940	43.333	90	48	2	37	125	61	148	1.17e-18	78.6
MS.gene70249.t1	MTR_4g120270	43.056	72	38	1	51	122	78	146	1.31e-18	80.5
MS.gene70249.t1	MTR_4g063070	43.210	81	45	1	43	122	298	378	1.62e-18	81.6
MS.gene70249.t1	MTR_1g043410	37.624	101	57	3	25	123	70	166	2.80e-18	80.1
MS.gene70249.t1	MTR_6g055030	46.053	76	39	1	52	125	96	171	3.97e-18	79.7
MS.gene70249.t1	MTR_6g087150	50.704	71	34	1	53	123	93	162	4.30e-18	80.1
MS.gene70249.t1	MTR_2g096350	42.529	87	45	2	75	161	130	211	5.32e-18	77.8
MS.gene70249.t1	MTR_8g066780	34.677	124	78	2	21	141	80	203	5.84e-18	79.7
MS.gene70249.t1	MTR_3g099650	42.308	78	43	1	51	126	105	182	1.04e-17	79.3
MS.gene70249.t1	MTR_7g076250	39.286	112	59	3	51	161	61	164	1.23e-17	76.3
MS.gene70249.t1	MTR_2g436100	38.095	84	51	1	39	122	79	161	2.35e-17	77.4
MS.gene70249.t1	MTR_3g464780	43.590	78	41	1	52	126	101	178	4.39e-17	76.3
MS.gene70249.t1	MTR_7g113800	39.394	99	50	3	37	126	69	166	4.56e-17	77.4
MS.gene70249.t1	MTR_4g056590	42.308	78	40	2	52	129	99	171	4.94e-17	74.7
MS.gene70249.t1	MTR_5g071930	50.000	76	34	2	52	127	65	136	5.09e-17	74.7
MS.gene70249.t1	MTR_3g071300	39.130	92	53	1	37	125	60	151	5.47e-17	74.3
MS.gene70249.t1	MTR_4g129420	41.772	79	46	0	48	126	112	190	6.28e-17	77.4
MS.gene70249.t1	MTR_1g492830	36.364	88	55	1	52	139	60	146	7.33e-17	75.1
MS.gene70249.t1	MTR_5g064980	38.043	92	53	2	52	141	107	196	9.60e-17	76.6
MS.gene70249.t1	MTR_7g116220	38.462	78	46	1	52	127	69	146	1.05e-16	73.6
MS.gene70249.t1	MTR_4g093830	33.645	107	61	2	53	159	126	222	1.11e-16	76.3
MS.gene70249.t1	MTR_7g108855	36.111	108	59	3	40	138	53	159	1.24e-16	73.9
MS.gene70249.t1	MTR_3g078470	36.364	99	61	2	28	125	52	149	3.03e-16	73.2
MS.gene70249.t1	MTR_5g030010	39.241	79	47	1	48	126	117	194	3.26e-16	75.1
MS.gene70249.t1	MTR_4g030070	34.091	88	56	1	43	130	75	160	3.28e-16	73.2
MS.gene70249.t1	MTR_5g064890	29.921	127	76	2	12	126	75	200	3.93e-16	74.7
MS.gene70249.t1	MTR_4g108830	35.354	99	60	2	26	123	122	217	4.94e-16	72.8
MS.gene70249.t1	MTR_3g078450	38.043	92	56	1	35	125	58	149	5.05e-16	72.8
MS.gene70249.t1	MTR_8g089880	37.500	80	49	1	53	132	127	205	5.72e-16	73.6
MS.gene70249.t1	MTR_5g066250	41.667	72	41	1	51	122	132	202	6.07e-16	74.3
MS.gene70249.t1	MTR_5g064990	39.744	78	45	1	51	126	112	189	7.39e-16	73.9
MS.gene70249.t1	MTR_3g100400	41.667	72	41	1	51	122	128	198	1.29e-15	73.6
MS.gene70249.t1	MTR_2g055790	37.778	90	52	1	37	126	61	146	1.42e-15	70.9
MS.gene70249.t1	MTR_2g009310	46.753	77	25	2	78	154	159	219	1.92e-15	72.8
MS.gene70249.t1	MTR_4g108830	32.710	107	68	2	18	123	86	189	2.07e-15	70.5
MS.gene70249.t1	MTR_5g034790	41.463	82	44	2	47	125	107	187	2.32e-15	72.4
MS.gene70249.t1	MTR_7g116180	38.462	78	46	1	50	125	64	141	2.55e-15	70.1
MS.gene70249.t1	MTR_1g070425	38.462	78	46	1	50	125	65	142	3.38e-15	69.7
MS.gene70249.t1	MTR_5g026730	36.190	105	52	4	37	137	56	149	3.91e-15	69.3
MS.gene70249.t1	MTR_3g091620	33.898	118	73	2	9	126	61	173	4.10e-15	71.6
MS.gene70249.t1	MTR_5g092790	41.463	82	45	2	43	122	147	227	4.10e-15	70.5
MS.gene70249.t1	MTR_3g086700	35.955	89	55	1	36	122	29	117	4.49e-15	69.7
MS.gene70249.t1	MTR_3g114500	37.349	83	48	2	40	122	35	113	6.28e-15	67.8
MS.gene70249.t1	MTR_1g070140	39.744	78	45	2	46	122	96	172	6.86e-15	70.9
MS.gene70249.t1	MTR_4g106680	35.354	99	57	2	49	141	102	199	6.99e-15	70.9
MS.gene70249.t1	MTR_8g063660	39.286	84	47	2	47	127	59	141	7.60e-15	70.9
MS.gene70249.t1	MTR_1g074190	36.047	86	46	2	52	130	98	181	9.23e-15	70.9
MS.gene70249.t1	MTR_7g079280	31.148	122	70	3	43	163	70	178	9.67e-15	68.6
MS.gene70249.t1	MTR_6g007670	36.082	97	50	3	50	144	5	91	1.03e-14	69.7
MS.gene70249.t1	MTR_6g007670	43.662	71	40	0	52	122	144	214	6.14e-13	64.7
MS.gene70249.t1	MTR_8g028465	33.981	103	52	3	53	155	90	176	1.17e-14	68.6
MS.gene70249.t1	MTR_2g090950	39.744	78	42	1	50	122	88	165	1.18e-14	69.3
MS.gene70249.t1	MTR_3g086730	40.000	80	46	1	48	125	41	120	1.23e-14	69.3
MS.gene70249.t1	MTR_2g090905	41.558	77	40	1	51	122	37	113	1.34e-14	67.0
MS.gene70249.t1	MTR_8g074900	41.333	75	39	1	51	125	156	225	1.47e-14	68.9
MS.gene70249.t1	MTR_1g056350	32.692	104	57	3	47	147	68	161	1.67e-14	67.8
MS.gene70249.t1	MTR_3g012550	26.573	143	91	4	17	157	46	176	2.22e-14	67.4
MS.gene70249.t1	MTR_6g007737	35.789	95	57	2	34	125	111	204	2.91e-14	69.3
MS.gene70249.t1	MTR_5g022920	38.961	77	46	1	50	125	158	234	3.15e-14	68.2
MS.gene70249.t1	MTR_6g007680	41.096	73	42	1	50	122	37	108	3.69e-14	66.2
MS.gene70249.t1	MTR_5g092750	41.463	82	45	2	43	122	147	227	5.19e-14	67.4
MS.gene70249.t1	MTR_8g099495	40.278	72	42	1	53	123	319	390	6.10e-14	68.6
MS.gene70249.t1	MTR_3g091200	39.437	71	42	1	53	122	303	373	6.15e-14	68.6
MS.gene70249.t1	MTR_5g009210	40.278	72	42	1	53	123	287	358	6.16e-14	68.6
MS.gene70249.t1	MTR_5g009190	37.805	82	50	1	43	123	256	337	6.20e-14	68.6
MS.gene70249.t1	MTR_7g059360	45.455	66	35	1	59	123	136	201	7.86e-14	67.0
MS.gene70249.t1	MTR_2g041200	56.250	48	21	0	75	122	104	151	8.36e-14	65.9
MS.gene70249.t1	MTR_5g009200	40.278	72	42	1	53	123	322	393	1.07e-13	67.8
MS.gene70249.t1	MTR_7g079290	32.143	84	54	1	52	132	124	207	1.28e-13	66.2
MS.gene70249.t1	MTR_8g032690	50.000	52	26	0	80	131	19	70	1.37e-13	63.5
MS.gene70249.t1	MTR_5g026720	53.488	43	20	0	80	122	93	135	1.43e-13	65.1
MS.gene70249.t1	MTR_5g076990	37.755	98	58	1	28	122	51	148	1.57e-13	67.0
MS.gene70249.t1	MTR_1g026070	37.500	72	41	2	51	122	46	113	2.07e-13	63.9
MS.gene70249.t1	MTR_3g087900	42.222	90	42	5	38	126	35	115	5.78e-13	63.2
MS.gene70249.t1	MTR_8g019710	60.000	45	18	0	78	122	52	96	6.65e-13	62.8
MS.gene70249.t1	MTR_8g072020	49.231	65	31	2	59	122	385	448	6.75e-13	65.9
MS.gene70249.t1	MTR_8g072020	49.231	65	31	2	59	122	424	487	7.46e-13	65.5
MS.gene70249.t1	MTR_3g078120	32.727	110	72	2	15	122	18	127	9.74e-13	62.4
MS.gene70249.t1	MTR_8g071815	53.488	43	20	0	80	122	90	132	9.95e-13	63.2
MS.gene70249.t1	MTR_2g079320	32.407	108	64	2	25	125	99	204	1.18e-12	63.5
MS.gene70249.t1	MTR_7g116200	36.842	76	46	1	47	122	62	135	1.18e-12	62.8
MS.gene70249.t1	MTR_8g099515	41.096	73	40	2	53	123	309	380	1.46e-12	64.7
MS.gene70249.t1	MTR_3g081030	35.802	81	50	1	59	139	100	178	1.64e-12	63.2
MS.gene70249.t1	MTR_4g022410	34.314	102	62	2	38	139	76	172	2.01e-12	62.4
MS.gene70249.t1	MTR_3g078540	42.857	63	35	1	67	128	66	128	2.14e-12	61.6
MS.gene70249.t1	MTR_3g078520	32.584	89	52	1	42	122	57	145	2.34e-12	62.0
MS.gene70249.t1	MTR_8g066740	35.714	98	60	1	28	122	51	148	4.12e-12	63.2
MS.gene70249.t1	MTR_7g117350	34.211	76	45	1	53	123	290	365	7.65e-12	62.4
MS.gene70249.t1	MTR_4g116460	53.333	45	19	2	79	122	440	483	7.68e-12	62.8
MS.gene70249.t1	MTR_4g116460	53.333	45	19	2	79	122	483	526	7.99e-12	62.8
MS.gene70249.t1	MTR_7g117350	34.211	76	45	1	53	123	282	357	8.15e-12	62.4
MS.gene70249.t1	MTR_1g090480	33.673	98	62	1	34	128	53	150	8.69e-12	60.5
MS.gene70249.t1	MTR_8g009190	33.735	83	53	1	46	126	69	151	1.29e-11	61.6
MS.gene70249.t1	MTR_6g055030	52.273	44	21	0	82	125	97	140	1.59e-11	61.2
MS.gene70249.t1	MTR_4g106960	54.000	50	22	1	73	122	171	219	1.68e-11	61.6
MS.gene70249.t1	MTR_4g106960	54.000	50	22	1	73	122	171	219	1.76e-11	61.2
MS.gene70249.t1	MTR_8g027980	33.333	75	48	1	56	128	93	167	1.95e-11	60.8
MS.gene70249.t1	MTR_8g027745	36.111	72	45	1	53	123	286	357	2.02e-11	61.2
MS.gene70249.t1	MTR_4g106960	54.000	50	22	1	73	122	228	276	2.12e-11	61.2
MS.gene70249.t1	MTR_4g106960	54.000	50	22	1	73	122	228	276	2.30e-11	61.2
MS.gene70249.t1	MTR_7g116210	35.526	76	47	1	47	122	4	77	2.56e-11	57.8
MS.gene70249.t1	MTR_2g044570	40.000	55	33	0	79	133	82	136	3.89e-11	60.1
MS.gene70249.t1	MTR_5g041310	56.818	44	18	1	79	122	114	156	8.91e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70249.t1	AT1G49200	57.143	119	47	1	50	164	106	224	2.65e-47	153
MS.gene70249.t1	AT1G49220	56.618	136	53	3	23	154	80	213	3.81e-47	154
MS.gene70249.t1	AT1G49210	57.627	118	46	1	51	164	106	223	6.62e-47	152
MS.gene70249.t1	AT1G49230	51.678	149	57	3	23	163	78	219	4.25e-46	150
MS.gene70249.t1	AT3G18773	48.630	146	70	3	23	164	74	218	2.80e-44	145
MS.gene70249.t1	AT5G05280	55.238	105	40	2	23	127	63	160	4.98e-34	118
MS.gene70249.t1	AT3G10910	49.533	107	50	2	23	126	55	160	3.51e-30	108
MS.gene70249.t1	AT5G01880	51.020	98	42	4	41	136	66	159	4.30e-28	102
MS.gene70249.t1	AT1G20823	47.475	99	49	1	31	126	59	157	1.15e-27	102
MS.gene70249.t1	AT1G76410	54.430	79	36	0	50	128	74	152	3.07e-27	101
MS.gene70249.t1	AT2G17450	49.383	81	35	1	51	125	67	147	8.89e-23	89.7
MS.gene70249.t1	AT5G47610	58.065	62	26	0	61	122	88	149	5.04e-22	87.0
MS.gene70249.t1	AT4G35480	41.667	120	52	3	51	156	70	185	7.25e-21	85.1
MS.gene70249.t1	AT1G72310	48.718	78	38	1	49	126	98	173	6.87e-20	84.7
MS.gene70249.t1	AT4G40070	41.176	102	56	2	34	131	74	175	2.43e-19	83.2
MS.gene70249.t1	AT1G72200	36.800	125	75	3	20	141	83	206	2.70e-19	84.0
MS.gene70249.t1	AT2G35000	36.697	109	65	2	22	126	73	181	6.03e-19	82.8
MS.gene70249.t1	AT3G05200	42.391	92	51	1	38	127	84	175	9.43e-19	82.4
MS.gene70249.t1	AT1G53820	44.156	77	41	1	49	125	91	165	1.36e-18	80.9
MS.gene70249.t1	AT4G17245	57.377	61	25	1	63	122	85	145	1.38e-18	78.2
MS.gene70249.t1	AT2G20030	47.887	71	36	1	53	123	98	167	4.13e-18	80.5
MS.gene70249.t1	AT3G16720	39.583	96	53	3	38	132	79	170	4.34e-18	79.7
MS.gene70249.t1	AT3G14320	47.887	71	35	1	53	123	63	131	4.71e-18	77.8
MS.gene70249.t1	AT4G09120	46.154	78	40	1	50	125	91	168	5.49e-18	79.7
MS.gene70249.t1	AT4G15975	34.400	125	79	2	35	159	33	154	8.35e-18	77.8
MS.gene70249.t1	AT4G09110	44.872	78	41	1	50	125	91	168	1.21e-17	78.2
MS.gene70249.t1	AT2G27940	40.230	87	49	2	52	138	114	197	1.25e-17	77.4
MS.gene70249.t1	AT5G27420	39.394	99	52	3	36	127	74	171	1.91e-17	78.6
MS.gene70249.t1	AT2G18650	44.578	83	43	1	47	126	90	172	1.93e-17	78.6
MS.gene70249.t1	AT1G74410	38.462	91	55	1	35	125	132	221	2.07e-17	76.6
MS.gene70249.t1	AT4G30400	41.333	75	44	0	48	122	102	176	3.61e-17	78.2
MS.gene70249.t1	AT4G28890	45.070	71	38	1	53	123	97	166	3.63e-17	77.8
MS.gene70249.t1	AT2G25410	44.828	87	46	1	38	122	283	369	3.90e-17	77.8
MS.gene70249.t1	AT1G35330	31.967	122	77	2	27	142	70	191	4.35e-17	77.0
MS.gene70249.t1	AT4G17905	54.098	61	23	1	78	138	153	208	4.39e-17	77.0
MS.gene70249.t1	AT1G28040	39.216	102	55	3	27	123	197	296	4.50e-17	76.6
MS.gene70249.t1	AT5G57750	31.618	136	92	1	7	141	48	183	4.72e-17	75.1
MS.gene70249.t1	AT4G17905	54.098	61	23	1	78	138	179	234	4.77e-17	77.0
MS.gene70249.t1	AT3G03550	59.184	49	20	0	78	126	157	205	8.43e-17	76.6
MS.gene70249.t1	AT5G17600	38.889	90	54	1	33	122	96	184	9.53e-17	76.3
MS.gene70249.t1	AT1G22500	35.455	110	68	2	32	138	67	176	1.24e-16	76.3
MS.gene70249.t1	AT2G46493	38.462	91	51	2	38	123	91	181	3.23e-16	72.4
MS.gene70249.t1	AT4G09130	43.421	76	41	1	52	125	90	165	4.54e-16	74.3
MS.gene70249.t1	AT4G09100	37.805	82	49	2	43	122	46	127	5.01e-16	70.9
MS.gene70249.t1	AT5G05810	43.056	72	40	1	51	122	118	188	5.55e-16	74.3
MS.gene70249.t1	AT3G18930	35.849	106	57	4	53	149	120	223	6.18e-16	74.3
MS.gene70249.t1	AT3G18930	35.849	106	57	4	53	149	120	223	6.18e-16	74.3
MS.gene70249.t1	AT3G18930	35.849	106	57	4	53	149	120	223	6.18e-16	74.3
MS.gene70249.t1	AT5G10380	39.241	79	47	1	48	126	104	181	1.51e-15	72.8
MS.gene70249.t1	AT3G19140	35.644	101	61	2	23	123	26	122	1.62e-15	69.7
MS.gene70249.t1	AT3G48030	38.750	80	43	2	49	125	98	174	1.83e-15	72.0
MS.gene70249.t1	AT3G48030	38.750	80	43	2	49	125	176	252	3.03e-15	72.0
MS.gene70249.t1	AT1G72220	57.778	45	19	0	78	122	175	219	3.09e-15	72.4
MS.gene70249.t1	AT2G35910	35.789	95	51	2	53	147	121	205	3.54e-15	70.5
MS.gene70249.t1	AT2G34990	36.082	97	59	2	28	122	65	160	3.75e-15	71.6
MS.gene70249.t1	AT2G34990	36.082	97	59	2	28	122	43	138	3.83e-15	71.6
MS.gene70249.t1	AT1G23980	35.354	99	56	2	35	126	93	190	4.31e-15	72.0
MS.gene70249.t1	AT2G35420	43.056	72	40	1	51	122	75	145	5.03e-15	70.5
MS.gene70249.t1	AT5G06490	34.653	101	51	2	53	147	96	187	5.57e-15	69.7
MS.gene70249.t1	AT4G10160	35.714	84	52	1	43	126	62	143	5.62e-15	70.1
MS.gene70249.t1	AT1G32361	33.588	131	76	5	34	158	108	233	6.11e-15	71.2
MS.gene70249.t1	AT5G42200	38.158	76	46	1	47	122	72	146	6.20e-15	68.6
MS.gene70249.t1	AT5G66070	44.000	75	39	2	51	125	150	221	7.22e-15	69.7
MS.gene70249.t1	AT5G66070	44.000	75	39	2	51	125	150	221	7.22e-15	69.7
MS.gene70249.t1	AT3G62690	34.167	120	68	3	37	154	71	181	1.10e-14	69.7
MS.gene70249.t1	AT5G66070	44.000	75	39	2	51	125	174	245	1.18e-14	69.3
MS.gene70249.t1	AT5G58580	36.957	92	56	1	35	126	95	184	1.24e-14	70.1
MS.gene70249.t1	AT2G46494	39.474	76	45	1	49	123	284	359	1.27e-14	70.5
MS.gene70249.t1	AT4G33565	52.941	51	24	0	78	128	215	265	1.28e-14	70.5
MS.gene70249.t1	AT2G46160	33.333	102	56	2	53	147	104	200	1.31e-14	68.9
MS.gene70249.t1	AT2G46160	33.333	102	56	2	53	147	104	200	1.31e-14	68.9
MS.gene70249.t1	AT4G10150	34.524	84	53	1	43	126	76	157	3.46e-14	68.2
MS.gene70249.t1	AT1G04360	36.709	79	44	1	50	122	98	176	3.97e-14	69.3
MS.gene70249.t1	AT4G35840	35.870	92	57	1	36	125	145	236	4.90e-14	67.8
MS.gene70249.t1	AT4G17920	39.744	78	45	1	50	125	78	155	4.94e-14	68.2
MS.gene70249.t1	AT5G53110	38.889	72	43	1	53	123	302	373	5.13e-14	68.9
MS.gene70249.t1	AT2G42360	37.333	75	47	0	48	122	76	150	6.46e-14	67.4
MS.gene70249.t1	AT5G36001	61.702	47	18	0	77	123	261	307	8.65e-14	67.8
MS.gene70249.t1	AT5G07040	34.722	72	46	1	53	123	66	137	1.42e-13	65.1
MS.gene70249.t1	AT5G40250	50.000	48	24	0	79	126	142	189	2.03e-13	67.0
MS.gene70249.t1	AT2G46495	34.343	99	61	2	27	123	267	363	2.07e-13	67.0
MS.gene70249.t1	AT1G33480	33.784	74	47	1	53	126	75	146	2.15e-13	66.2
MS.gene70249.t1	AT3G11110	37.349	83	52	0	40	122	67	149	2.38e-13	64.3
MS.gene70249.t1	AT2G46495	34.343	99	61	2	27	123	239	335	2.64e-13	66.6
MS.gene70249.t1	AT2G18670	34.667	75	49	0	48	122	78	152	2.77e-13	64.7
MS.gene70249.t1	AT2G47560	41.096	73	42	1	51	122	78	150	3.32e-13	65.5
MS.gene70249.t1	AT2G17730	36.842	76	46	1	50	123	164	239	4.49e-13	65.1
MS.gene70249.t1	AT5G43420	56.818	44	19	0	79	122	137	180	4.54e-13	66.2
MS.gene70249.t1	AT2G34000	36.000	75	45	1	52	123	60	134	6.04e-13	63.2
MS.gene70249.t1	AT3G61550	29.600	125	69	3	46	160	92	207	6.19e-13	64.3
MS.gene70249.t1	AT2G42350	37.333	75	45	1	48	122	70	142	1.19e-12	63.5
MS.gene70249.t1	AT4G26400	44.118	68	30	2	55	122	223	282	1.80e-12	64.3
MS.gene70249.t1	AT4G26400	44.118	68	30	2	55	122	223	282	1.80e-12	64.3
MS.gene70249.t1	AT1G53010	50.000	50	25	0	79	128	129	178	4.83e-12	61.2
MS.gene70249.t1	AT1G35630	42.254	71	39	2	54	123	207	276	5.36e-12	62.8
MS.gene70249.t1	AT5G56340	38.272	81	42	2	42	122	228	300	5.71e-12	63.2
MS.gene70249.t1	AT4G11360	40.000	85	39	3	44	126	59	133	1.48e-11	59.7
MS.gene70249.t1	AT3G20395	36.264	91	54	2	36	125	129	216	1.62e-11	60.5
MS.gene70249.t1	AT5G52150	32.292	96	58	2	26	115	104	198	2.13e-11	60.1
MS.gene70249.t1	AT2G37580	48.936	47	24	0	76	122	138	184	2.23e-11	60.5
MS.gene70249.t1	AT3G47990	59.091	44	17	1	79	122	177	219	2.32e-11	60.8
MS.gene70249.t1	AT3G47990	59.091	44	17	1	79	122	177	219	2.32e-11	60.8
MS.gene70249.t1	AT3G47990	59.091	44	17	1	79	122	177	219	2.32e-11	60.8
MS.gene70249.t1	AT3G47990	59.091	44	17	1	79	122	234	276	2.39e-11	61.2
MS.gene70249.t1	AT3G47990	59.091	44	17	1	79	122	218	260	2.69e-11	60.8
MS.gene70249.t1	AT2G03000	51.064	47	22	1	79	125	480	525	4.50e-11	60.5
MS.gene70249.t1	AT4G26580	34.409	93	55	2	38	125	242	333	5.05e-11	60.1
MS.gene70249.t1	AT4G26580	34.409	93	55	2	38	125	242	333	5.05e-11	60.1
MS.gene70249.t1	AT4G11370	41.379	87	35	5	44	126	60	134	5.30e-11	58.2
MS.gene70249.t1	AT4G30370	35.616	73	46	1	51	123	87	158	5.66e-11	58.5
MS.gene70249.t1	AT5G46650	35.366	82	50	2	47	125	79	160	8.00e-11	59.3
MS.gene70249.t1	AT2G40830	41.429	70	39	2	56	125	167	234	8.69e-11	59.3
MS.gene70249.t1	AT2G40830	41.429	70	39	2	56	125	167	234	8.69e-11	59.3
MS.gene70249.t1	AT2G40830	41.429	70	39	2	56	125	167	234	8.69e-11	59.3
MS.gene70249.t1	AT2G40830	41.429	70	39	2	56	125	167	234	8.69e-11	59.3

Find 35 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCATCATAAGGTGTGGTTTA+AGG	0.114506	4.1:+8749584	MS.gene70249:intron
TTTCTTCTTGATTCCTTTGT+TGG	0.230023	4.1:-8749674	None:intergenic
CGAAGAAGAGTCGTTGTTTA+GGG	0.283250	4.1:-8749620	None:intergenic
TCGAAGAAGAGTCGTTGTTT+AGG	0.298317	4.1:-8749621	None:intergenic
CAGTGCTAGAAACCGTTATA+AGG	0.329221	4.1:+8749950	MS.gene70249:CDS
TGAAATCCATGGTTGCATTT+AGG	0.353073	4.1:-8749814	None:intergenic
TGAGTTGAAACTACCAAGTT+TGG	0.353182	4.1:+8749732	MS.gene70249:CDS
TCCAAAGCTTCATGCTCTAA+TGG	0.359601	4.1:-8749979	None:intergenic
TCTAGCACTGGCACAACAAT+TGG	0.383583	4.1:-8749937	None:intergenic
ACCATTAGAGCATGAAGCTT+TGG	0.405256	4.1:+8749978	MS.gene70249:CDS
CAGCAACACATCACAACAAT+TGG	0.425791	4.1:+8749651	MS.gene70249:CDS
AAACTTGTAGAAAGATTGTT+GGG	0.429889	4.1:+8749905	MS.gene70249:CDS
GAAACTTGTAGAAAGATTGT+TGG	0.437661	4.1:+8749904	MS.gene70249:CDS
CTTATAACGGTTTCTAGCAC+TGG	0.474285	4.1:-8749949	None:intergenic
AGAGTTCACAAAGGGTGAAA+AGG	0.489010	4.1:+8749780	MS.gene70249:CDS
TAATGGTTGGACCCTTATAA+CGG	0.489916	4.1:-8749962	None:intergenic
ATGTGCGTTGCATTGACAAA+TGG	0.492413	4.1:+8749836	MS.gene70249:CDS
AGTGCTAGAAACCGTTATAA+GGG	0.501196	4.1:+8749951	MS.gene70249:CDS
AAGCTTCATGCTCTAATGGT+TGG	0.509803	4.1:-8749975	None:intergenic
TTGTGGAATCTCACACAAGA+AGG	0.511006	4.1:+8749392	MS.gene70249:CDS
ATGGAGAAGTGGTGAAGTAA+AGG	0.523047	4.1:-8749362	None:intergenic
ACTTCTCCATGAGCTTCTTG+TGG	0.539026	4.1:+8749375	MS.gene70249:CDS
ACACACTCTGTATCCAAACT+TGG	0.542352	4.1:-8749745	None:intergenic
TCTTTCTACAAGTTTCAAGA+AGG	0.552794	4.1:-8749897	None:intergenic
TCAGCTGAATAGCTCACTGT+GGG	0.566806	4.1:-8749712	None:intergenic
TTGCATTGACAAATGGCTAA+AGG	0.569827	4.1:+8749843	MS.gene70249:CDS
GAGATTCCACAAGAAGCTCA+TGG	0.584576	4.1:-8749381	None:intergenic
TGTCTCTCAGAGTTCACAAA+GGG	0.586259	4.1:+8749772	MS.gene70249:CDS
AGAAGGCACTGTCTACACTT+AGG	0.588478	4.1:-8749880	None:intergenic
ACAAGAAGCTCATGGAGAAG+TGG	0.600282	4.1:-8749373	None:intergenic
TCACAACAATTGGCCAACAA+AGG	0.611058	4.1:+8749661	MS.gene70249:CDS
TGCAACGCACATGAAATCCA+TGG	0.639037	4.1:-8749825	None:intergenic
CTCAGCTGAATAGCTCACTG+TGG	0.665359	4.1:-8749713	None:intergenic
ATGTCTCTCAGAGTTCACAA+AGG	0.686490	4.1:+8749771	MS.gene70249:CDS
CAGCTGAATAGCTCACTGTG+GGG	0.730132	4.1:-8749711	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCAAATGTTATAATGATAC+TGG	+	chr4.1:8749519-8749538	MS.gene70249:intron	20.0%
!!!	TATGGTTTTTTGTTAAAACA+GGG	-	chr4.1:8749462-8749481	None:intergenic	20.0%
!!!	TTATGGTTTTTTGTTAAAAC+AGG	-	chr4.1:8749463-8749482	None:intergenic	20.0%
!	AAACTTGTAGAAAGATTGTT+GGG	+	chr4.1:8749905-8749924	MS.gene70249:CDS	25.0%
!	AATATGAGTCTGTTGAATTA+TGG	-	chr4.1:8749480-8749499	None:intergenic	25.0%
!	AATTCAACAGACTCATATTT+CGG	+	chr4.1:8749481-8749500	MS.gene70249:intron	25.0%
!!!	ATGGTTTTTTGTTAAAACAG+GGG	-	chr4.1:8749461-8749480	None:intergenic	25.0%
	GAAACTTGTAGAAAGATTGT+TGG	+	chr4.1:8749904-8749923	MS.gene70249:CDS	30.0%
	TCTTTCTACAAGTTTCAAGA+AGG	-	chr4.1:8749900-8749919	None:intergenic	30.0%
	TTTCTTCTTGATTCCTTTGT+TGG	-	chr4.1:8749677-8749696	None:intergenic	30.0%
!	TTAGTGATTGATGATCAACT+GGG	-	chr4.1:8749432-8749451	None:intergenic	30.0%
	AGTGCTAGAAACCGTTATAA+GGG	+	chr4.1:8749951-8749970	MS.gene70249:CDS	35.0%
	TAATGGTTGGACCCTTATAA+CGG	-	chr4.1:8749965-8749984	None:intergenic	35.0%
	TGAAATCCATGGTTGCATTT+AGG	-	chr4.1:8749817-8749836	None:intergenic	35.0%
	TTAAACTCCATCATAAGGTG+TGG	+	chr4.1:8749577-8749596	MS.gene70249:intron	35.0%
	TTGCCTTAAACTCCATCATA+AGG	+	chr4.1:8749572-8749591	MS.gene70249:intron	35.0%
!	ACACCTTATGATGGAGTTTA+AGG	-	chr4.1:8749578-8749597	None:intergenic	35.0%
!	ATTTTGCCTAAATGCAACCA+TGG	+	chr4.1:8749808-8749827	MS.gene70249:CDS	35.0%
!	GTTAGTGATTGATGATCAAC+TGG	-	chr4.1:8749433-8749452	None:intergenic	35.0%
!	TGAGTTGAAACTACCAAGTT+TGG	+	chr4.1:8749732-8749751	MS.gene70249:CDS	35.0%
!	TTGCATTGACAAATGGCTAA+AGG	+	chr4.1:8749843-8749862	MS.gene70249:CDS	35.0%
	AAGCTTCATGCTCTAATGGT+TGG	-	chr4.1:8749978-8749997	None:intergenic	40.0%
	ACACACTCTGTATCCAAACT+TGG	-	chr4.1:8749748-8749767	None:intergenic	40.0%
	ACCATTAGAGCATGAAGCTT+TGG	+	chr4.1:8749978-8749997	MS.gene70249:CDS	40.0%
	AGAGTTCACAAAGGGTGAAA+AGG	+	chr4.1:8749780-8749799	MS.gene70249:CDS	40.0%
	ATGGAGAAGTGGTGAAGTAA+AGG	-	chr4.1:8749365-8749384	None:intergenic	40.0%
	ATGTCTCTCAGAGTTCACAA+AGG	+	chr4.1:8749771-8749790	MS.gene70249:CDS	40.0%
	CAGCAACACATCACAACAAT+TGG	+	chr4.1:8749651-8749670	MS.gene70249:CDS	40.0%
	CAGTGCTAGAAACCGTTATA+AGG	+	chr4.1:8749950-8749969	MS.gene70249:CDS	40.0%
	CCATCATAAGGTGTGGTTTA+AGG	+	chr4.1:8749584-8749603	MS.gene70249:intron	40.0%
	CCTTAAACCACACCTTATGA+TGG	-	chr4.1:8749587-8749606	None:intergenic	40.0%
	CGAAGAAGAGTCGTTGTTTA+GGG	-	chr4.1:8749623-8749642	None:intergenic	40.0%
	TCACAACAATTGGCCAACAA+AGG	+	chr4.1:8749661-8749680	MS.gene70249:CDS	40.0%
	TCCAAAGCTTCATGCTCTAA+TGG	-	chr4.1:8749982-8750001	None:intergenic	40.0%
	TCGAAGAAGAGTCGTTGTTT+AGG	-	chr4.1:8749624-8749643	None:intergenic	40.0%
	TGTCTCTCAGAGTTCACAAA+GGG	+	chr4.1:8749772-8749791	MS.gene70249:CDS	40.0%
	TTGTGGAATCTCACACAAGA+AGG	+	chr4.1:8749392-8749411	MS.gene70249:CDS	40.0%
!	ATGTGCGTTGCATTGACAAA+TGG	+	chr4.1:8749836-8749855	MS.gene70249:CDS	40.0%
!!	CTTATAACGGTTTCTAGCAC+TGG	-	chr4.1:8749952-8749971	None:intergenic	40.0%
	ACAAGAAGCTCATGGAGAAG+TGG	-	chr4.1:8749376-8749395	None:intergenic	45.0%
	ACTTCTCCATGAGCTTCTTG+TGG	+	chr4.1:8749375-8749394	MS.gene70249:CDS	45.0%
	GAGATTCCACAAGAAGCTCA+TGG	-	chr4.1:8749384-8749403	None:intergenic	45.0%
	TCAGCTGAATAGCTCACTGT+GGG	-	chr4.1:8749715-8749734	None:intergenic	45.0%
	TGCAACGCACATGAAATCCA+TGG	-	chr4.1:8749828-8749847	None:intergenic	45.0%
!!	AGAAGGCACTGTCTACACTT+AGG	-	chr4.1:8749883-8749902	None:intergenic	45.0%
!!	TCTAGCACTGGCACAACAAT+TGG	-	chr4.1:8749940-8749959	None:intergenic	45.0%
	CAGCTGAATAGCTCACTGTG+GGG	-	chr4.1:8749714-8749733	None:intergenic	50.0%
	CTCAGCTGAATAGCTCACTG+TGG	-	chr4.1:8749716-8749735	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	8749346	8750023	8749346	ID=MS.gene70249
chr4.1	mRNA	8749346	8750023	8749346	ID=MS.gene70249.t1;Parent=MS.gene70249
chr4.1	exon	8749346	8749413	8749346	ID=MS.gene70249.t1.exon1;Parent=MS.gene70249.t1
chr4.1	CDS	8749346	8749413	8749346	ID=cds.MS.gene70249.t1;Parent=MS.gene70249.t1
chr4.1	exon	8749594	8750023	8749594	ID=MS.gene70249.t1.exon2;Parent=MS.gene70249.t1
chr4.1	CDS	8749594	8750023	8749594	ID=cds.MS.gene70249.t1;Parent=MS.gene70249.t1

Gene Sequence

>MS.gene70249

ATGTATACTTCTACTTCCTTTACTTCACCACTTCTCCATGAGCTTCTTGTGGAATCTCACACAAGAAGGTTACTCATTCAAAACCCAGTTGATCATCAATCACTAACAAACTCCCCTGTTTTAACAAAAAACCATAATTCAACAGACTCATATTTCGGAGCTCGTGAATTTAATTCAAATGTTATAATGATACTGGCAGTTCTATTGTGTGCTCTTATTTGCTCACTTGCCTTAAACTCCATCATAAGGTGTGGTTTAAGGTTTTCAAACGTAGCCCTAAACAACGACTCTTCTTCGAGTTCGAGCAGCAACACATCACAACAATTGGCCAACAAAGGAATCAAGAAGAAAGCTCTCAAAACATTCCCCACAGTGAGCTATTCAGCTGAGTTGAAACTACCAAGTTTGGATACAGAGTGTGTGATATGTCTCTCAGAGTTCACAAAGGGTGAAAAGGTGCGCATTTTGCCTAAATGCAACCATGGATTTCATGTGCGTTGCATTGACAAATGGCTAAAGGAACACTCATCATGTCCTAAGTGTAGACAGTGCCTTCTTGAAACTTGTAGAAAGATTGTTGGGTCACAACAACCAATTGTTGTGCCAGTGCTAGAAACCGTTATAAGGGTCCAACCATTAGAGCATGAAGCTTTGGAACGTAACTACAGAGAAATATAA

Protein sequence

>MS.gene70249.t1

MYTSTSFTSPLLHELLVESHTRRCGLRFSNVALNNDSSSSSSSNTSQQLANKGIKKKALKTFPTVSYSAELKLPSLDTECVICLSEFTKGEKVRILPKCNHGFHVRCIDKWLKEHSSCPKCRQCLLETCRKIVGSQQPIVVPVLETVIRVQPLEHEALERNYREI