AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28425

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28425.t1 MTR_8g092990 90.909 231 21 0 1 231 1 231 1.05e-135 380
MS.gene28425.t1 MTR_8g092840 80.973 226 42 1 1 226 1 225 6.62e-123 348
MS.gene28425.t1 MTR_8g092970 81.938 227 31 2 1 227 1 217 1.48e-122 347
MS.gene28425.t1 MTR_8g093010 79.039 229 36 5 1 227 1 219 2.55e-122 346
MS.gene28425.t1 MTR_8g092930 80.176 227 43 2 1 227 1 225 5.60e-119 338
MS.gene28425.t1 MTR_8g092985 81.690 213 36 1 1 213 1 210 2.21e-118 336
MS.gene28425.t1 MTR_8g092960 76.991 226 41 2 1 226 1 215 3.66e-116 330
MS.gene28425.t1 MTR_3g095560 62.447 237 75 4 1 230 1 230 8.00e-96 280
MS.gene28425.t1 MTR_3g094000 50.000 146 67 2 54 198 36 176 1.38e-41 140
MS.gene28425.t1 MTR_7g073200 49.315 146 58 4 47 187 19 153 2.26e-39 134
MS.gene28425.t1 MTR_5g024730 44.444 162 73 6 55 208 26 178 1.84e-35 125
MS.gene28425.t1 MTR_3g116000 48.201 139 65 4 59 195 26 159 3.30e-34 121
MS.gene28425.t1 MTR_8g073120 44.056 143 68 4 55 194 19 152 8.65e-32 115
MS.gene28425.t1 MTR_4g094725 43.200 125 65 2 61 184 30 149 1.23e-28 105
MS.gene28425.t1 MTR_2g035800 38.760 129 73 2 56 184 23 145 1.40e-27 103
MS.gene28425.t1 MTR_3g071300 34.146 164 88 4 57 213 22 172 2.20e-22 90.5
MS.gene28425.t1 MTR_1g043410 28.659 164 104 4 62 216 35 194 2.67e-20 87.8
MS.gene28425.t1 MTR_7g113800 33.775 151 78 5 46 187 29 166 1.33e-19 86.3
MS.gene28425.t1 MTR_8g023400 45.570 79 40 2 114 191 92 168 4.97e-19 84.3
MS.gene28425.t1 MTR_4g120270 43.836 73 38 1 111 183 77 146 1.33e-18 82.4
MS.gene28425.t1 MTR_5g082940 47.368 76 39 1 112 186 73 148 1.84e-18 79.7
MS.gene28425.t1 MTR_1g082550 31.613 155 83 5 43 187 32 173 2.05e-18 83.2
MS.gene28425.t1 MTR_7g117540 31.111 135 91 1 55 189 17 149 2.29e-18 82.0
MS.gene28425.t1 MTR_3g110190 33.333 132 78 4 62 189 15 140 2.39e-18 82.8
MS.gene28425.t1 MTR_6g087150 33.577 137 90 1 48 184 27 162 6.30e-18 82.0
MS.gene28425.t1 MTR_1g100737 45.783 83 41 2 112 190 90 172 1.02e-17 78.6
MS.gene28425.t1 MTR_2g436100 31.210 157 86 5 42 183 12 161 1.77e-17 79.7
MS.gene28425.t1 MTR_3g078450 35.652 115 71 2 89 202 51 163 2.27e-17 78.2
MS.gene28425.t1 MTR_6g055030 36.975 119 66 3 67 183 57 168 3.55e-17 79.3
MS.gene28425.t1 MTR_1g492830 39.474 76 46 0 113 188 60 135 3.74e-17 77.8
MS.gene28425.t1 MTR_5g064990 30.000 150 100 4 53 199 55 202 4.59e-17 79.3
MS.gene28425.t1 MTR_2g096350 60.417 48 19 0 136 183 130 177 4.61e-17 77.4
MS.gene28425.t1 MTR_7g116220 40.000 80 46 1 111 188 67 146 4.64e-17 76.3
MS.gene28425.t1 MTR_3g114500 33.871 124 67 5 64 183 1 113 4.82e-17 75.1
MS.gene28425.t1 MTR_5g064980 31.746 126 81 2 63 186 60 182 6.77e-17 79.0
MS.gene28425.t1 MTR_3g464780 43.590 78 41 1 113 187 101 178 1.02e-16 77.0
MS.gene28425.t1 MTR_7g076250 36.283 113 62 3 112 223 61 164 1.14e-16 75.5
MS.gene28425.t1 MTR_3g099650 30.597 134 85 3 57 187 54 182 1.43e-16 78.2
MS.gene28425.t1 MTR_2g055790 35.772 123 68 3 66 187 34 146 1.48e-16 75.5
MS.gene28425.t1 MTR_4g056590 39.326 89 47 3 111 199 97 178 1.66e-16 75.1
MS.gene28425.t1 MTR_4g063070 42.105 76 43 1 109 183 303 378 2.23e-16 77.4
MS.gene28425.t1 MTR_4g030070 36.364 88 54 1 104 191 75 160 2.96e-16 75.1
MS.gene28425.t1 MTR_8g066780 26.425 193 120 3 59 229 48 240 3.53e-16 76.6
MS.gene28425.t1 MTR_4g129420 41.772 79 46 0 109 187 112 190 5.02e-16 77.0
MS.gene28425.t1 MTR_5g071930 40.426 94 50 2 113 206 65 152 6.85e-16 73.6
MS.gene28425.t1 MTR_5g030010 36.275 102 62 2 107 208 115 213 8.81e-16 75.9
MS.gene28425.t1 MTR_8g089880 37.500 80 49 1 114 193 127 205 1.01e-15 74.7
MS.gene28425.t1 MTR_5g076990 35.433 127 76 3 62 183 23 148 1.20e-15 74.7
MS.gene28425.t1 MTR_4g108830 34.694 98 62 1 87 184 122 217 1.71e-15 73.2
MS.gene28425.t1 MTR_7g116180 40.000 75 43 1 114 186 67 141 1.75e-15 72.0
MS.gene28425.t1 MTR_3g078470 37.113 97 59 2 91 186 54 149 2.29e-15 72.8
MS.gene28425.t1 MTR_1g026070 30.000 120 77 3 64 183 1 113 2.31e-15 70.5
MS.gene28425.t1 MTR_1g070425 40.000 75 43 1 114 186 68 142 2.43e-15 71.6
MS.gene28425.t1 MTR_5g066250 41.667 72 41 1 112 183 132 202 3.09e-15 74.3
MS.gene28425.t1 MTR_6g007737 32.117 137 78 4 62 186 71 204 4.23e-15 73.9
MS.gene28425.t1 MTR_3g100400 41.667 72 41 1 112 183 128 198 4.42e-15 73.9
MS.gene28425.t1 MTR_7g108855 36.957 92 49 2 115 197 67 158 4.96e-15 71.2
MS.gene28425.t1 MTR_4g093830 32.407 108 62 2 114 221 126 222 5.44e-15 73.6
MS.gene28425.t1 MTR_5g064890 31.034 116 77 2 74 187 86 200 6.91e-15 73.2
MS.gene28425.t1 MTR_2g009310 60.000 45 18 0 139 183 159 203 7.75e-15 73.2
MS.gene28425.t1 MTR_1g070140 38.158 76 47 0 108 183 97 172 7.76e-15 72.8
MS.gene28425.t1 MTR_4g108830 34.375 96 61 1 89 184 96 189 9.76e-15 70.5
MS.gene28425.t1 MTR_5g026720 28.467 137 84 4 54 183 6 135 9.93e-15 69.7
MS.gene28425.t1 MTR_3g012550 28.030 132 92 3 53 183 21 150 1.29e-14 69.7
MS.gene28425.t1 MTR_1g074190 32.231 121 72 3 81 191 61 181 1.59e-14 72.0
MS.gene28425.t1 MTR_6g007680 43.662 71 39 1 113 183 39 108 1.96e-14 68.9
MS.gene28425.t1 MTR_5g026730 27.612 134 79 4 59 183 12 136 2.32e-14 68.9
MS.gene28425.t1 MTR_8g074900 41.333 75 39 1 112 186 156 225 2.35e-14 70.1
MS.gene28425.t1 MTR_2g090905 44.286 70 27 1 140 209 70 127 2.54e-14 67.8
MS.gene28425.t1 MTR_5g034790 40.260 77 45 1 110 186 112 187 3.30e-14 71.2
MS.gene28425.t1 MTR_3g086700 36.458 96 55 2 94 183 22 117 3.75e-14 68.9
MS.gene28425.t1 MTR_5g092790 41.667 72 41 1 113 183 156 227 4.02e-14 69.7
MS.gene28425.t1 MTR_3g091620 33.636 110 60 3 111 208 98 206 4.78e-14 70.5
MS.gene28425.t1 MTR_7g079280 31.858 113 62 3 114 225 80 178 4.78e-14 68.2
MS.gene28425.t1 MTR_8g028465 32.692 104 53 3 114 217 90 176 5.01e-14 68.6
MS.gene28425.t1 MTR_8g063660 31.159 138 83 3 62 188 5 141 6.08e-14 70.1
MS.gene28425.t1 MTR_1g056350 33.684 95 59 2 110 201 70 163 6.45e-14 67.8
MS.gene28425.t1 MTR_8g099495 38.961 77 46 1 108 183 313 389 6.58e-14 70.5
MS.gene28425.t1 MTR_5g022920 39.474 76 45 1 112 186 159 234 7.30e-14 68.9
MS.gene28425.t1 MTR_5g009190 38.272 81 49 1 104 183 256 336 1.01e-13 69.7
MS.gene28425.t1 MTR_3g086730 38.750 80 47 1 109 186 41 120 1.08e-13 68.6
MS.gene28425.t1 MTR_6g007670 40.260 77 41 2 110 183 1 75 1.09e-13 68.6
MS.gene28425.t1 MTR_6g007670 45.070 71 39 0 113 183 144 214 2.88e-13 67.4
MS.gene28425.t1 MTR_2g090950 39.744 78 42 1 111 183 88 165 1.19e-13 68.2
MS.gene28425.t1 MTR_2g041200 46.774 62 33 0 122 183 90 151 1.26e-13 67.0
MS.gene28425.t1 MTR_8g032690 43.077 65 34 1 141 205 19 80 1.52e-13 65.1
MS.gene28425.t1 MTR_5g009210 38.750 80 48 1 105 183 278 357 1.79e-13 68.9
MS.gene28425.t1 MTR_3g091200 39.437 71 42 1 114 183 303 373 1.87e-13 68.9
MS.gene28425.t1 MTR_3g087900 48.529 68 30 3 121 187 52 115 2.39e-13 65.9
MS.gene28425.t1 MTR_7g059360 47.368 57 30 0 128 184 145 201 2.55e-13 67.4
MS.gene28425.t1 MTR_7g079290 32.941 85 52 2 113 193 124 207 2.73e-13 67.0
MS.gene28425.t1 MTR_5g092750 43.056 72 40 1 113 183 156 227 4.03e-13 67.0
MS.gene28425.t1 MTR_4g106680 37.333 75 46 1 109 183 101 174 4.39e-13 67.4
MS.gene28425.t1 MTR_5g009200 40.845 71 41 1 114 183 322 392 6.37e-13 67.4
MS.gene28425.t1 MTR_8g099515 42.254 71 40 1 114 183 309 379 1.05e-12 67.0
MS.gene28425.t1 MTR_8g019710 60.000 45 18 0 139 183 52 96 1.27e-12 63.5
MS.gene28425.t1 MTR_7g096690 26.471 136 79 5 62 183 14 142 1.57e-12 66.2
MS.gene28425.t1 MTR_3g078120 28.889 135 71 3 62 183 5 127 1.76e-12 63.5
MS.gene28425.t1 MTR_2g079320 32.110 109 69 1 83 186 96 204 1.79e-12 64.3
MS.gene28425.t1 MTR_8g071815 53.488 43 20 0 141 183 90 132 3.61e-12 63.2
MS.gene28425.t1 MTR_7g116200 32.090 134 69 5 65 183 9 135 4.56e-12 62.8
MS.gene28425.t1 MTR_4g022410 29.688 128 63 2 62 183 49 155 6.30e-12 62.8
MS.gene28425.t1 MTR_8g066740 31.496 127 81 3 62 183 23 148 6.41e-12 64.3
MS.gene28425.t1 MTR_4g116460 53.333 45 19 2 140 183 440 483 1.31e-11 63.9
MS.gene28425.t1 MTR_4g116460 53.333 45 19 2 140 183 483 526 1.41e-11 63.9
MS.gene28425.t1 MTR_3g081030 34.343 99 55 2 120 218 100 188 2.47e-11 61.6
MS.gene28425.t1 MTR_4g106960 54.000 50 22 1 134 183 171 219 3.82e-11 62.4
MS.gene28425.t1 MTR_4g106960 54.000 50 22 1 134 183 171 219 4.14e-11 62.0
MS.gene28425.t1 MTR_4g106960 54.000 50 22 1 134 183 228 276 4.34e-11 62.4
MS.gene28425.t1 MTR_6g055030 53.659 41 19 0 143 183 97 137 4.45e-11 61.6
MS.gene28425.t1 MTR_4g106960 54.000 50 22 1 134 183 228 276 4.71e-11 62.0
MS.gene28425.t1 MTR_8g027745 34.615 78 50 1 107 183 279 356 5.17e-11 62.0
MS.gene28425.t1 MTR_8g027980 33.333 75 48 1 117 189 93 167 5.40e-11 61.2
MS.gene28425.t1 MTR_3g078540 41.270 63 36 1 128 189 66 128 6.88e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28425.t1 AT1G49230 49.550 222 86 4 11 225 17 219 3.31e-66 204
MS.gene28425.t1 AT1G49210 46.890 209 100 4 21 226 23 223 2.36e-57 182
MS.gene28425.t1 AT1G49220 47.739 199 93 5 21 216 23 213 1.17e-56 181
MS.gene28425.t1 AT1G49200 45.933 209 102 5 21 226 24 224 3.37e-56 179
MS.gene28425.t1 AT3G18773 50.292 171 79 3 60 226 50 218 1.22e-52 169
MS.gene28425.t1 AT5G05280 51.429 140 61 2 58 197 37 169 3.03e-42 141
MS.gene28425.t1 AT3G10910 52.632 133 59 3 58 187 29 160 2.77e-40 137
MS.gene28425.t1 AT1G76410 44.056 143 77 2 47 189 13 152 4.19e-36 126
MS.gene28425.t1 AT1G20823 45.865 133 68 2 58 187 26 157 5.12e-35 124
MS.gene28425.t1 AT5G01880 43.590 156 76 5 43 197 15 159 1.94e-28 105
MS.gene28425.t1 AT2G17450 40.690 145 64 5 59 194 25 156 2.17e-28 106
MS.gene28425.t1 AT4G35480 39.073 151 71 5 59 194 23 167 4.52e-27 103
MS.gene28425.t1 AT4G17245 40.299 134 63 3 52 183 27 145 3.08e-26 100
MS.gene28425.t1 AT5G47610 37.879 132 70 3 52 183 30 149 4.82e-26 99.8
MS.gene28425.t1 AT4G40070 34.395 157 93 4 65 216 49 200 2.16e-20 88.2
MS.gene28425.t1 AT1G22500 34.307 137 84 3 54 187 31 164 3.53e-19 85.5
MS.gene28425.t1 AT1G72200 32.099 162 85 5 58 213 59 201 1.05e-18 84.3
MS.gene28425.t1 AT1G72310 47.436 78 39 1 110 187 98 173 4.22e-18 82.0
MS.gene28425.t1 AT2G35000 33.083 133 85 2 59 187 49 181 5.65e-18 82.0
MS.gene28425.t1 AT5G57750 32.283 127 81 2 62 184 40 165 5.73e-18 79.7
MS.gene28425.t1 AT2G46494 31.081 148 96 3 42 183 211 358 5.77e-18 82.0
MS.gene28425.t1 AT1G74410 39.560 91 54 1 96 186 132 221 8.23e-18 79.3
MS.gene28425.t1 AT3G14320 47.887 71 35 1 114 184 63 131 1.64e-17 78.2
MS.gene28425.t1 AT1G53820 40.217 92 52 2 110 200 91 180 4.82e-17 79.0
MS.gene28425.t1 AT3G16720 40.476 84 47 2 110 193 90 170 5.01e-17 78.6
MS.gene28425.t1 AT3G05200 42.222 90 48 2 113 200 98 185 8.97e-17 79.0
MS.gene28425.t1 AT1G04360 30.827 133 80 3 62 183 45 176 1.28e-16 78.2
MS.gene28425.t1 AT1G28040 36.538 104 63 2 83 183 192 295 2.49e-16 76.6
MS.gene28425.t1 AT4G09100 32.787 122 75 3 64 183 11 127 2.89e-16 73.2
MS.gene28425.t1 AT2G20030 45.714 70 37 1 114 183 98 166 3.05e-16 77.0
MS.gene28425.t1 AT4G17905 66.667 45 15 0 139 183 179 223 3.32e-16 76.6
MS.gene28425.t1 AT4G17905 66.667 45 15 0 139 183 153 197 3.39e-16 76.3
MS.gene28425.t1 AT2G27940 36.782 87 53 1 108 194 109 193 4.13e-16 75.1
MS.gene28425.t1 AT4G09120 45.205 73 38 1 113 183 93 165 4.48e-16 76.3
MS.gene28425.t1 AT3G03550 64.444 45 16 0 139 183 157 201 5.20e-16 76.3
MS.gene28425.t1 AT1G23980 31.788 151 82 5 54 187 44 190 5.44e-16 76.3
MS.gene28425.t1 AT4G09110 32.231 121 74 2 65 183 51 165 5.76e-16 75.9
MS.gene28425.t1 AT1G32361 34.752 141 81 5 84 223 101 231 6.05e-16 75.9
MS.gene28425.t1 AT4G15975 38.636 88 52 1 96 183 33 118 6.58e-16 74.7
MS.gene28425.t1 AT2G18650 42.308 78 45 0 110 187 95 172 6.98e-16 76.3
MS.gene28425.t1 AT3G62690 36.538 104 54 3 115 216 88 181 8.82e-16 74.7
MS.gene28425.t1 AT5G17600 43.056 72 40 1 112 183 114 184 9.56e-16 75.5
MS.gene28425.t1 AT4G30400 40.000 75 45 0 109 183 102 176 1.01e-15 75.9
MS.gene28425.t1 AT5G42200 40.541 74 43 1 110 183 74 146 1.12e-15 72.4
MS.gene28425.t1 AT5G27420 36.893 103 48 3 111 210 92 180 1.12e-15 75.5
MS.gene28425.t1 AT2G42360 32.000 125 72 2 62 183 36 150 1.32e-15 73.6
MS.gene28425.t1 AT5G66070 42.105 76 41 2 111 186 149 221 1.62e-15 73.2
MS.gene28425.t1 AT5G66070 42.105 76 41 2 111 186 149 221 1.62e-15 73.2
MS.gene28425.t1 AT4G10160 32.540 126 75 3 65 187 25 143 1.69e-15 73.2
MS.gene28425.t1 AT1G35330 41.026 78 44 1 111 186 97 174 2.23e-15 74.3
MS.gene28425.t1 AT5G66070 42.105 76 41 2 111 186 173 245 2.34e-15 73.2
MS.gene28425.t1 AT4G28890 42.254 71 40 1 114 184 97 166 2.91e-15 74.7
MS.gene28425.t1 AT4G09130 42.667 75 41 1 111 183 88 162 4.40e-15 73.6
MS.gene28425.t1 AT2G35420 29.677 155 100 6 64 218 30 175 5.09e-15 72.4
MS.gene28425.t1 AT5G05810 41.667 72 41 1 112 183 118 188 5.86e-15 73.6
MS.gene28425.t1 AT2G34990 28.105 153 102 3 59 209 12 158 9.10e-15 72.4
MS.gene28425.t1 AT2G34990 28.105 153 102 3 59 209 34 180 9.25e-15 72.4
MS.gene28425.t1 AT1G72220 46.970 66 31 1 139 200 175 240 9.25e-15 73.2
MS.gene28425.t1 AT2G46493 33.663 101 64 2 86 183 80 180 9.26e-15 70.5
MS.gene28425.t1 AT2G17730 41.333 75 42 1 112 184 165 239 1.07e-14 71.2
MS.gene28425.t1 AT5G06490 33.333 102 52 2 114 209 96 187 1.22e-14 70.5
MS.gene28425.t1 AT4G17920 31.061 132 84 2 62 186 24 155 1.58e-14 71.6
MS.gene28425.t1 AT3G48030 37.662 77 48 0 110 186 98 174 1.66e-14 71.2
MS.gene28425.t1 AT3G48030 32.090 134 75 3 69 186 119 252 2.46e-14 71.6
MS.gene28425.t1 AT4G35840 41.333 75 42 1 112 184 160 234 3.05e-14 70.1
MS.gene28425.t1 AT3G61550 28.966 145 76 4 88 222 80 207 3.81e-14 69.3
MS.gene28425.t1 AT4G33565 52.941 51 24 0 139 189 215 265 3.86e-14 70.9
MS.gene28425.t1 AT5G10380 37.975 79 48 1 109 187 104 181 4.03e-14 70.5
MS.gene28425.t1 AT2G25410 42.529 87 47 2 100 183 283 369 4.41e-14 70.9
MS.gene28425.t1 AT5G43420 27.273 143 83 2 62 183 38 180 5.01e-14 70.9
MS.gene28425.t1 AT2G46160 32.039 103 57 2 114 209 104 200 6.66e-14 68.6
MS.gene28425.t1 AT2G46160 32.039 103 57 2 114 209 104 200 6.66e-14 68.6
MS.gene28425.t1 AT2G47560 42.466 73 41 1 112 183 78 150 7.22e-14 68.9
MS.gene28425.t1 AT5G58580 40.789 76 45 0 112 187 109 184 1.12e-13 69.3
MS.gene28425.t1 AT2G18670 36.000 75 48 0 109 183 78 152 1.19e-13 67.4
MS.gene28425.t1 AT5G36001 63.043 46 17 0 138 183 261 306 1.48e-13 68.9
MS.gene28425.t1 AT3G18930 42.105 76 37 2 140 214 155 224 1.57e-13 69.3
MS.gene28425.t1 AT3G18930 42.105 76 37 2 140 214 155 224 1.57e-13 69.3
MS.gene28425.t1 AT3G18930 42.105 76 37 2 140 214 155 224 1.57e-13 69.3
MS.gene28425.t1 AT2G35910 35.227 88 56 1 114 201 121 207 1.58e-13 67.8
MS.gene28425.t1 AT5G07040 34.177 79 51 1 106 183 58 136 3.36e-13 65.5
MS.gene28425.t1 AT2G34000 37.333 75 44 1 113 184 60 134 4.01e-13 65.1
MS.gene28425.t1 AT5G53110 39.437 71 42 1 114 183 302 372 4.75e-13 67.8
MS.gene28425.t1 AT2G46495 40.789 76 43 1 110 183 287 362 4.78e-13 67.8
MS.gene28425.t1 AT4G10150 30.233 129 84 3 63 187 31 157 5.00e-13 66.6
MS.gene28425.t1 AT2G46495 40.789 76 43 1 110 183 259 334 5.25e-13 67.8
MS.gene28425.t1 AT3G19140 40.845 71 40 1 114 184 54 122 6.07e-13 64.3
MS.gene28425.t1 AT5G40250 50.000 48 24 0 140 187 142 189 7.02e-13 67.4
MS.gene28425.t1 AT1G33480 28.713 101 69 2 114 214 75 172 2.35e-12 65.1
MS.gene28425.t1 AT2G37580 34.483 87 51 2 97 183 104 184 2.53e-12 64.7
MS.gene28425.t1 AT3G11110 36.145 83 53 0 101 183 67 149 2.79e-12 63.2
MS.gene28425.t1 AT2G42350 30.841 107 62 3 77 183 48 142 5.02e-12 63.5
MS.gene28425.t1 AT4G30370 35.616 73 46 1 112 184 87 158 9.59e-12 62.0
MS.gene28425.t1 AT5G56340 38.272 81 42 2 103 183 228 300 9.80e-12 64.3
MS.gene28425.t1 AT4G26400 42.647 68 31 2 116 183 223 282 1.03e-11 63.9
MS.gene28425.t1 AT4G26400 42.647 68 31 2 116 183 223 282 1.03e-11 63.9
MS.gene28425.t1 AT3G20395 32.283 127 81 3 60 186 95 216 1.25e-11 62.4
MS.gene28425.t1 AT1G53010 50.000 50 25 0 140 189 129 178 1.33e-11 61.6
MS.gene28425.t1 AT5G46650 30.323 155 93 6 45 186 8 160 1.62e-11 63.2
MS.gene28425.t1 AT4G26580 36.709 79 44 2 113 186 256 333 4.02e-11 62.0
MS.gene28425.t1 AT4G26580 36.709 79 44 2 113 186 256 333 4.02e-11 62.0
MS.gene28425.t1 AT2G03000 52.273 44 20 1 140 183 480 522 4.91e-11 62.4
MS.gene28425.t1 AT3G47990 54.000 50 22 1 134 183 228 276 5.86e-11 61.6
MS.gene28425.t1 AT3G47990 54.000 50 22 1 134 183 171 219 5.93e-11 61.6
MS.gene28425.t1 AT3G47990 54.000 50 22 1 134 183 171 219 5.93e-11 61.6
MS.gene28425.t1 AT3G47990 54.000 50 22 1 134 183 171 219 5.93e-11 61.6
MS.gene28425.t1 AT3G47990 54.000 50 22 1 134 183 212 260 6.14e-11 61.6
MS.gene28425.t1 AT4G11360 35.714 84 50 2 102 183 48 129 8.81e-11 58.9

Find 38 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCAACCAACTCATATTT+TGG 0.110117 4.4:-8806132 MS.gene28425:CDS
AATATGAGTTGGTTGAATTT+TGG 0.155497 4.4:+8806136 None:intergenic
TCTGGCACTGGTAACAAAAT+TGG 0.215829 4.4:+8805673 None:intergenic
TGGATTGCTATAATAATTTC+TGG 0.238268 4.4:+8805655 None:intergenic
ATTGATGTTCAATTGAATTT+TGG 0.255066 4.4:+8806193 None:intergenic
CGAGTTGAATCTACCAAGTT+TGG 0.287389 4.4:-8805881 MS.gene28425:CDS
CTTGAAACTTGTCGAAAGAT+TGG 0.318725 4.4:-8805712 MS.gene28425:CDS
TGAAAACCATGGTTGCATTT+AGG 0.347715 4.4:+8805799 None:intergenic
TTTGGCTATTTGTGAAAATA+CGG 0.358119 4.4:+8806154 None:intergenic
ACCACTTGAGCACGAAGCTT+TGG 0.379749 4.4:-8805632 MS.gene28425:CDS
TTGCATTAAACTTCATCATA+AGG 0.411192 4.4:-8806041 MS.gene28425:CDS
CAACAACAATCCACCTCAAT+TGG 0.419352 4.4:-8805962 MS.gene28425:CDS
ATGTTCGTTGCATTGACAAA+TGG 0.437642 4.4:-8805777 MS.gene28425:CDS
GCTATAATAATTTCTGGCAC+TGG 0.465203 4.4:+8805661 None:intergenic
TCGGTGGAATAGCTCATTGT+AGG 0.466374 4.4:+8805901 None:intergenic
ATTCCTTTGTTGACCAATTG+AGG 0.479348 4.4:+8805949 None:intergenic
TTGCATTGACAAATGGCTAA+AGG 0.492896 4.4:-8805770 MS.gene28425:CDS
TTGTAAAATCTCACACAAGA+AGG 0.493207 4.4:-8806224 MS.gene28425:CDS
AGAGTTCACAAAAGGTGAAA+AGG 0.495916 4.4:-8805833 MS.gene28425:CDS
AAGCTTCGTGCTCAAGTGGT+TGG 0.506524 4.4:+8805635 None:intergenic
TTGGCTATTTGTGAAAATAC+GGG 0.508540 4.4:+8806155 None:intergenic
TCCAAAGCTTCGTGCTCAAG+TGG 0.522894 4.4:+8805631 None:intergenic
ATGGAGAAGTGGTGAAGTAA+AGG 0.523047 4.4:+8806254 None:intergenic
TCGAAAGATTGGTGGGTCGC+AGG 0.526432 4.4:-8805701 MS.gene28425:CDS
AGAAGACATTGTCTACACTT+AGG 0.531664 4.4:+8805733 None:intergenic
ACACACTCTGTATCCAAACT+TGG 0.542352 4.4:+8805868 None:intergenic
TGTCTCTCAGAGTTCACAAA+AGG 0.544000 4.4:-8805841 MS.gene28425:CDS
GAAACTTGTCGAAAGATTGG+TGG 0.551880 4.4:-8805709 MS.gene28425:CDS
TATGATGAAGTTTAATGCAA+GGG 0.555043 4.4:+8806044 None:intergenic
ACGAGCTCCAAAATATGAGT+TGG 0.556220 4.4:+8806125 None:intergenic
TTATGATGAAGTTTAATGCA+AGG 0.569193 4.4:+8806043 None:intergenic
CCTTTGTTGACCAATTGAGG+TGG 0.571148 4.4:+8805952 None:intergenic
ACAAGAAGCTCATGGAGAAG+TGG 0.576844 4.4:+8806243 None:intergenic
AAACTTGTCGAAAGATTGGT+GGG 0.592454 4.4:-8805708 MS.gene28425:CDS
CCACCTCAATTGGTCAACAA+AGG 0.601823 4.4:-8805952 MS.gene28425:CDS
CAAACTTGGTAGATTCAACT+CGG 0.621643 4.4:+8805882 None:intergenic
TGCAACGAACATGAAAACCA+TGG 0.695842 4.4:+8805788 None:intergenic
ACTTGGTAGATTCAACTCGG+TGG 0.705125 4.4:+8805885 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGCTACATTTAAAAATCTTA+AGG + chr4.4:8805856-8805875 None:intergenic 20.0%
!!! ATTGATGTTCAATTGAATTT+TGG + chr4.4:8805677-8805696 None:intergenic 20.0%
! TATGATGAAGTTTAATGCAA+GGG + chr4.4:8805826-8805845 None:intergenic 25.0%
! TGGATTGCTATAATAATTTC+TGG + chr4.4:8806215-8806234 None:intergenic 25.0%
! TTATGATGAAGTTTAATGCA+AGG + chr4.4:8805827-8805846 None:intergenic 25.0%
! TTGCATTAAACTTCATCATA+AGG - chr4.4:8805826-8805845 MS.gene28425:CDS 25.0%
! TTTGGCTATTTGTGAAAATA+CGG + chr4.4:8805716-8805735 None:intergenic 25.0%
!! AATTCAACCAACTCATATTT+TGG - chr4.4:8805735-8805754 MS.gene28425:CDS 25.0%
!!! AATATGAGTTGGTTGAATTT+TGG + chr4.4:8805734-8805753 None:intergenic 25.0%
TTGGCTATTTGTGAAAATAC+GGG + chr4.4:8805715-8805734 None:intergenic 30.0%
TTGTAAAATCTCACACAAGA+AGG - chr4.4:8805643-8805662 MS.gene28425:CDS 30.0%
AAACTTGTCGAAAGATTGGT+GGG - chr4.4:8806159-8806178 MS.gene28425:CDS 35.0%
AGAAGACATTGTCTACACTT+AGG + chr4.4:8806137-8806156 None:intergenic 35.0%
AGAGTTCACAAAAGGTGAAA+AGG - chr4.4:8806034-8806053 MS.gene28425:CDS 35.0%
ATTCCTTTGTTGACCAATTG+AGG + chr4.4:8805921-8805940 None:intergenic 35.0%
CAAACTTGGTAGATTCAACT+CGG + chr4.4:8805988-8806007 None:intergenic 35.0%
CTTGAAACTTGTCGAAAGAT+TGG - chr4.4:8806155-8806174 MS.gene28425:CDS 35.0%
TGAAAACCATGGTTGCATTT+AGG + chr4.4:8806071-8806090 None:intergenic 35.0%
! ATGTTCGTTGCATTGACAAA+TGG - chr4.4:8806090-8806109 MS.gene28425:CDS 35.0%
! ATTTTGCCTAAATGCAACCA+TGG - chr4.4:8806062-8806081 MS.gene28425:CDS 35.0%
! GAGATTTTACAAGAAGCTCA+TGG + chr4.4:8805635-8805654 None:intergenic 35.0%
! TTGCATTGACAAATGGCTAA+AGG - chr4.4:8806097-8806116 MS.gene28425:CDS 35.0%
!! GCTATAATAATTTCTGGCAC+TGG + chr4.4:8806209-8806228 None:intergenic 35.0%
ACACACTCTGTATCCAAACT+TGG + chr4.4:8806002-8806021 None:intergenic 40.0%
ACGAGCTCCAAAATATGAGT+TGG + chr4.4:8805745-8805764 None:intergenic 40.0%
ATGGAGAAGTGGTGAAGTAA+AGG + chr4.4:8805616-8805635 None:intergenic 40.0%
CAACAACAATCCACCTCAAT+TGG - chr4.4:8805905-8805924 MS.gene28425:CDS 40.0%
GAAACTTGTCGAAAGATTGG+TGG - chr4.4:8806158-8806177 MS.gene28425:CDS 40.0%
TGCAACGAACATGAAAACCA+TGG + chr4.4:8806082-8806101 None:intergenic 40.0%
TGTCTCTCAGAGTTCACAAA+AGG - chr4.4:8806026-8806045 MS.gene28425:CDS 40.0%
! CGAGTTGAATCTACCAAGTT+TGG - chr4.4:8805986-8806005 MS.gene28425:CDS 40.0%
!! TCTGGCACTGGTAACAAAAT+TGG + chr4.4:8806197-8806216 None:intergenic 40.0%
ACAAGAAGCTCATGGAGAAG+TGG + chr4.4:8805627-8805646 None:intergenic 45.0%
ACTTGGTAGATTCAACTCGG+TGG + chr4.4:8805985-8806004 None:intergenic 45.0%
CCACCTCAATTGGTCAACAA+AGG - chr4.4:8805915-8805934 MS.gene28425:CDS 45.0%
CCTTTGTTGACCAATTGAGG+TGG + chr4.4:8805918-8805937 None:intergenic 45.0%
TCGGTGGAATAGCTCATTGT+AGG + chr4.4:8805969-8805988 None:intergenic 45.0%
AAGCTTCGTGCTCAAGTGGT+TGG + chr4.4:8806235-8806254 None:intergenic 50.0%
ACCACTTGAGCACGAAGCTT+TGG - chr4.4:8806235-8806254 MS.gene28425:CDS 50.0%
TCCAAAGCTTCGTGCTCAAG+TGG + chr4.4:8806239-8806258 None:intergenic 50.0%
! TCGAAAGATTGGTGGGTCGC+AGG - chr4.4:8806166-8806185 MS.gene28425:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 8805597 8806292 8805597 ID=MS.gene28425
chr4.4 mRNA 8805597 8806292 8805597 ID=MS.gene28425.t1;Parent=MS.gene28425
chr4.4 exon 8805597 8806292 8805597 ID=MS.gene28425.t1.exon1;Parent=MS.gene28425.t1
chr4.4 CDS 8805597 8806292 8805597 ID=cds.MS.gene28425.t1;Parent=MS.gene28425.t1
Gene Sequence

>MS.gene28425

ATGTATACTTCTACTTCCTTTACTTCACCACTTCTCCATGAGCTTCTTGTAAAATCTCACACAAGAAGGTTACTCTTCCAAAATTCAATTGAACATCAATCACTAAAAATCAACTCCCGTATTTTCACAAATAGCCAAAATTCAACCAACTCATATTTTGGAGCTCGTGAATTTGATTCAAATGTTGTGATGATACTTGCAGTTCTATTATGTGCTTTTATTTGTTCCCTTGCATTAAACTTCATCATAAGGCGTGCCTTAAGATTTTTAAATGTAGCTCTCAACAACGACTCTTCTTCGAGTTCAAGCAACAACAATCCACCTCAATTGGTCAACAAAGGAATCAAGAAGAAAGCTCTCAAGAAATTTCCTACAATGAGCTATTCCACCGAGTTGAATCTACCAAGTTTGGATACAGAGTGTGTGATATGTCTCTCAGAGTTCACAAAAGGTGAAAAGGTGCGCATTTTGCCTAAATGCAACCATGGTTTTCATGTTCGTTGCATTGACAAATGGCTAAAGGAACACTCATCATGTCCTAAGTGTAGACAATGTCTTCTTGAAACTTGTCGAAAGATTGGTGGGTCGCAGGTGCAACCAATTTTGTTACCAGTGCCAGAAATTATTATAGCAATCCAACCACTTGAGCACGAAGCTTTGGAACGAAACTATAGAGAAATACAAGCTAACAATTAA
Protein sequence

>MS.gene28425.t1

MYTSTSFTSPLLHELLVKSHTRRLLFQNSIEHQSLKINSRIFTNSQNSTNSYFGAREFDSNVVMILAVLLCAFICSLALNFIIRRALRFLNVALNNDSSSSSSNNNPPQLVNKGIKKKALKKFPTMSYSTELNLPSLDTECVICLSEFTKGEKVRILPKCNHGFHVRCIDKWLKEHSSCPKCRQCLLETCRKIGGSQVQPILLPVPEIIIAIQPLEHEALERNYREIQANN