AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28429

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28429.t1 MTR_8g092930 90.909 143 13 0 23 165 83 225 2.65e-81 240
MS.gene28429.t1 MTR_8g092970 86.014 143 12 1 23 165 83 217 5.31e-80 236
MS.gene28429.t1 MTR_8g093010 81.119 143 19 2 23 165 85 219 1.66e-79 235
MS.gene28429.t1 MTR_8g092960 58.986 217 34 2 1 164 1 215 5.76e-77 228
MS.gene28429.t1 MTR_8g092840 83.916 143 22 1 23 164 83 225 3.68e-76 227
MS.gene28429.t1 MTR_8g092990 85.417 144 20 1 23 165 84 227 1.61e-70 213
MS.gene28429.t1 MTR_8g092985 81.752 137 23 1 15 151 76 210 7.10e-70 210
MS.gene28429.t1 MTR_3g095560 68.243 148 41 2 23 165 81 227 1.45e-69 211
MS.gene28429.t1 MTR_5g024730 46.154 130 56 5 23 145 55 177 1.87e-29 107
MS.gene28429.t1 MTR_3g094000 46.957 115 56 2 23 136 66 176 6.91e-29 105
MS.gene28429.t1 MTR_3g116000 55.294 85 35 2 50 133 77 159 2.73e-27 101
MS.gene28429.t1 MTR_8g073120 47.423 97 45 3 40 132 58 152 2.90e-27 101
MS.gene28429.t1 MTR_7g073200 50.467 107 40 5 23 125 56 153 4.59e-25 95.5
MS.gene28429.t1 MTR_4g094725 54.430 79 35 1 45 122 71 149 5.57e-24 92.0
MS.gene28429.t1 MTR_2g035800 53.425 73 33 1 50 122 74 145 3.57e-23 90.1
MS.gene28429.t1 MTR_3g110190 45.679 81 43 1 47 127 61 140 1.39e-20 86.7
MS.gene28429.t1 MTR_6g055030 44.211 95 50 2 38 129 83 177 1.65e-20 86.3
MS.gene28429.t1 MTR_1g100737 46.988 83 40 1 50 128 90 172 2.64e-20 83.6
MS.gene28429.t1 MTR_8g023400 45.570 79 40 2 52 129 92 168 1.93e-19 83.6
MS.gene28429.t1 MTR_4g120270 44.737 76 35 2 48 121 76 146 2.39e-19 82.4
MS.gene28429.t1 MTR_3g099650 43.590 78 42 1 50 125 105 182 1.49e-18 81.6
MS.gene28429.t1 MTR_7g117540 45.333 75 39 1 53 127 77 149 1.67e-18 80.5
MS.gene28429.t1 MTR_1g043410 33.846 130 77 3 27 154 72 194 2.18e-18 80.5
MS.gene28429.t1 MTR_5g064990 30.323 155 101 4 10 159 74 226 3.03e-18 80.9
MS.gene28429.t1 MTR_3g078450 35.088 114 71 2 28 140 52 163 3.12e-18 78.6
MS.gene28429.t1 MTR_6g087150 50.704 71 34 1 52 122 93 162 3.70e-18 80.5
MS.gene28429.t1 MTR_4g056590 40.000 90 47 3 48 137 96 178 4.43e-18 77.8
MS.gene28429.t1 MTR_5g082940 44.872 78 42 1 48 124 71 148 4.43e-18 77.0
MS.gene28429.t1 MTR_7g116220 35.455 110 59 3 49 146 67 176 4.58e-18 77.4
MS.gene28429.t1 MTR_3g078470 37.755 98 60 1 28 124 52 149 4.74e-18 78.2
MS.gene28429.t1 MTR_5g071930 45.745 94 45 3 51 144 65 152 5.07e-18 77.4
MS.gene28429.t1 MTR_7g076250 35.833 120 67 3 43 161 54 164 5.68e-18 77.4
MS.gene28429.t1 MTR_7g113800 33.103 145 74 5 7 139 51 184 7.59e-18 79.3
MS.gene28429.t1 MTR_1g082550 33.884 121 75 2 7 125 56 173 1.01e-17 79.3
MS.gene28429.t1 MTR_2g096350 61.702 47 18 0 75 121 131 177 1.39e-17 76.6
MS.gene28429.t1 MTR_3g464780 42.353 85 46 1 44 125 94 178 1.47e-17 77.4
MS.gene28429.t1 MTR_4g063070 43.373 83 42 2 42 121 298 378 1.51e-17 79.0
MS.gene28429.t1 MTR_3g071300 36.752 117 69 3 11 124 37 151 1.98e-17 75.5
MS.gene28429.t1 MTR_5g064980 41.304 92 47 2 35 124 96 182 2.97e-17 77.8
MS.gene28429.t1 MTR_4g129420 43.421 76 43 0 50 125 115 190 3.49e-17 78.2
MS.gene28429.t1 MTR_2g436100 40.000 80 47 1 42 121 83 161 3.53e-17 77.0
MS.gene28429.t1 MTR_1g492830 38.158 76 47 0 51 126 60 135 6.82e-17 75.1
MS.gene28429.t1 MTR_5g066250 39.773 88 47 2 39 121 116 202 7.95e-17 77.0
MS.gene28429.t1 MTR_4g093830 33.333 108 61 2 52 159 126 222 1.13e-16 76.3
MS.gene28429.t1 MTR_5g030010 38.710 93 54 2 52 144 122 211 2.20e-16 75.5
MS.gene28429.t1 MTR_8g089880 37.500 80 49 1 52 131 127 205 2.83e-16 74.3
MS.gene28429.t1 MTR_8g066780 31.724 145 91 3 4 144 67 207 3.14e-16 74.7
MS.gene28429.t1 MTR_5g026730 34.579 107 65 3 44 146 55 160 5.07e-16 71.6
MS.gene28429.t1 MTR_2g055790 40.449 89 49 1 37 125 62 146 6.13e-16 72.0
MS.gene28429.t1 MTR_7g116180 40.789 76 43 1 51 124 66 141 7.14e-16 71.6
MS.gene28429.t1 MTR_3g100400 41.667 72 41 1 50 121 128 198 9.33e-16 73.9
MS.gene28429.t1 MTR_4g030070 33.708 89 57 1 41 129 74 160 1.12e-15 72.0
MS.gene28429.t1 MTR_7g108855 36.957 92 49 2 53 135 67 158 1.24e-15 71.2
MS.gene28429.t1 MTR_1g070425 40.789 76 43 1 51 124 67 142 1.25e-15 70.9
MS.gene28429.t1 MTR_2g009310 60.000 45 18 0 77 121 159 203 1.49e-15 73.2
MS.gene28429.t1 MTR_3g114500 40.964 83 44 3 40 121 35 113 1.83e-15 68.9
MS.gene28429.t1 MTR_5g064890 53.061 49 23 0 77 125 152 200 3.02e-15 72.0
MS.gene28429.t1 MTR_8g032690 40.580 69 38 1 79 147 19 84 5.31e-15 67.4
MS.gene28429.t1 MTR_2g090950 39.744 78 42 1 49 121 88 165 7.83e-15 69.7
MS.gene28429.t1 MTR_3g091620 38.961 77 46 1 49 125 98 173 8.08e-15 70.9
MS.gene28429.t1 MTR_2g090905 41.558 77 40 1 50 121 37 113 8.11e-15 67.4
MS.gene28429.t1 MTR_5g009210 39.506 81 48 1 42 121 277 357 8.18e-15 70.9
MS.gene28429.t1 MTR_1g070140 38.961 77 47 0 45 121 96 172 1.08e-14 70.5
MS.gene28429.t1 MTR_5g034790 41.558 77 40 2 50 124 114 187 1.18e-14 70.5
MS.gene28429.t1 MTR_3g086700 39.286 84 46 2 43 121 34 117 1.46e-14 68.2
MS.gene28429.t1 MTR_3g012550 29.245 106 73 2 17 121 46 150 1.53e-14 67.8
MS.gene28429.t1 MTR_4g108830 35.714 98 60 2 26 122 122 217 1.60e-14 68.6
MS.gene28429.t1 MTR_1g056350 33.000 100 63 2 44 140 66 164 1.60e-14 67.8
MS.gene28429.t1 MTR_1g074190 30.357 112 63 3 23 121 64 173 1.62e-14 70.1
MS.gene28429.t1 MTR_8g028465 36.842 76 45 1 47 122 85 157 1.63e-14 68.2
MS.gene28429.t1 MTR_5g009190 37.805 82 50 1 42 122 256 337 2.25e-14 69.7
MS.gene28429.t1 MTR_8g074900 40.000 75 40 1 50 124 156 225 2.65e-14 68.2
MS.gene28429.t1 MTR_5g092790 42.466 73 39 2 51 121 156 227 2.83e-14 68.2
MS.gene28429.t1 MTR_6g007737 38.667 75 45 1 50 124 131 204 3.05e-14 69.3
MS.gene28429.t1 MTR_3g086730 39.080 87 48 2 43 124 34 120 3.13e-14 68.2
MS.gene28429.t1 MTR_8g063660 37.662 77 47 1 50 126 66 141 3.16e-14 69.3
MS.gene28429.t1 MTR_4g106680 38.889 72 43 1 50 121 104 174 3.19e-14 68.9
MS.gene28429.t1 MTR_4g108830 33.019 106 68 2 18 122 86 189 3.45e-14 67.4
MS.gene28429.t1 MTR_6g007670 42.254 71 39 1 51 121 7 75 5.84e-14 67.8
MS.gene28429.t1 MTR_6g007670 43.662 71 40 0 51 121 144 214 1.25e-12 63.9
MS.gene28429.t1 MTR_7g079280 31.858 113 62 3 52 163 80 178 6.04e-14 66.2
MS.gene28429.t1 MTR_7g059360 42.029 69 39 1 55 122 133 201 6.40e-14 67.4
MS.gene28429.t1 MTR_8g099495 40.278 72 42 1 52 122 319 390 7.55e-14 68.6
MS.gene28429.t1 MTR_5g026720 53.488 43 20 0 79 121 93 135 9.28e-14 65.5
MS.gene28429.t1 MTR_2g041200 49.091 55 28 0 67 121 97 151 1.23e-13 65.5
MS.gene28429.t1 MTR_5g076990 38.776 98 56 2 28 121 51 148 1.34e-13 67.4
MS.gene28429.t1 MTR_5g022920 36.842 76 47 1 50 124 159 234 1.39e-13 66.2
MS.gene28429.t1 MTR_7g079290 43.103 58 33 0 74 131 150 207 1.46e-13 65.9
MS.gene28429.t1 MTR_6g007680 40.845 71 41 1 51 121 39 108 1.63e-13 64.7
MS.gene28429.t1 MTR_3g091200 38.028 71 43 1 52 121 303 373 2.01e-13 67.0
MS.gene28429.t1 MTR_5g009200 37.037 81 50 1 42 121 312 392 2.55e-13 67.0
MS.gene28429.t1 MTR_1g026070 35.616 73 43 1 49 121 45 113 2.73e-13 63.5
MS.gene28429.t1 MTR_5g092750 55.769 52 22 1 70 121 177 227 2.98e-13 65.5
MS.gene28429.t1 MTR_3g087900 47.692 65 29 3 62 125 55 115 4.00e-13 63.5
MS.gene28429.t1 MTR_8g019710 57.778 45 19 0 77 121 52 96 7.62e-13 62.4
MS.gene28429.t1 MTR_8g071815 53.488 43 20 0 79 121 90 132 8.78e-13 63.2
MS.gene28429.t1 MTR_8g072020 47.692 65 32 2 58 121 385 448 9.01e-13 65.5
MS.gene28429.t1 MTR_8g072020 47.692 65 32 2 58 121 424 487 1.00e-12 65.1
MS.gene28429.t1 MTR_8g099515 40.789 76 42 2 48 121 305 379 2.13e-12 64.3
MS.gene28429.t1 MTR_3g078120 34.524 84 54 1 39 121 44 127 2.43e-12 61.6
MS.gene28429.t1 MTR_7g116200 32.609 92 56 2 30 121 50 135 2.53e-12 62.0
MS.gene28429.t1 MTR_8g066740 36.735 98 58 2 28 121 51 148 4.77e-12 62.8
MS.gene28429.t1 MTR_7g117350 35.526 76 44 1 52 122 290 365 5.05e-12 63.2
MS.gene28429.t1 MTR_7g117350 35.526 76 44 1 52 122 282 357 5.48e-12 62.8
MS.gene28429.t1 MTR_8g027745 35.000 80 50 2 44 121 277 356 6.60e-12 62.8
MS.gene28429.t1 MTR_6g055030 50.000 50 24 1 81 129 97 146 8.43e-12 62.0
MS.gene28429.t1 MTR_2g079320 32.075 106 66 2 25 124 99 204 8.55e-12 61.2
MS.gene28429.t1 MTR_8g027980 33.333 75 48 1 55 127 93 167 1.13e-11 61.6
MS.gene28429.t1 MTR_3g078540 42.857 63 35 1 66 127 66 128 1.53e-11 59.3
MS.gene28429.t1 MTR_4g022410 46.429 56 30 0 66 121 100 155 1.73e-11 60.1
MS.gene28429.t1 MTR_2g044570 40.741 54 32 0 78 131 82 135 2.82e-11 60.5
MS.gene28429.t1 MTR_8g009190 46.939 49 26 0 77 125 103 151 2.84e-11 60.8
MS.gene28429.t1 MTR_7g096690 32.500 80 51 2 45 121 63 142 3.04e-11 60.8
MS.gene28429.t1 MTR_5g096410 36.458 96 46 3 43 124 232 326 3.27e-11 60.5
MS.gene28429.t1 MTR_5g096410 36.458 96 46 3 43 124 241 335 3.59e-11 60.5
MS.gene28429.t1 MTR_5g096410 36.458 96 46 3 43 124 244 338 3.80e-11 60.5
MS.gene28429.t1 MTR_4g106960 55.556 45 19 1 77 121 176 219 4.41e-11 60.5
MS.gene28429.t1 MTR_5g010100 32.000 100 64 3 28 125 31 128 4.94e-11 57.8
MS.gene28429.t1 MTR_3g081030 33.333 90 56 2 42 131 86 171 5.13e-11 59.3
MS.gene28429.t1 MTR_4g106960 55.556 45 19 1 77 121 176 219 5.33e-11 60.1
MS.gene28429.t1 MTR_4g106960 55.556 45 19 1 77 121 233 276 5.77e-11 60.1
MS.gene28429.t1 MTR_4g106960 55.556 45 19 1 77 121 233 276 6.39e-11 60.1
MS.gene28429.t1 MTR_3g078520 33.708 89 58 1 34 121 57 145 6.43e-11 58.2
MS.gene28429.t1 MTR_7g116210 48.837 43 22 0 79 121 35 77 7.05e-11 56.6
MS.gene28429.t1 MTR_4g129335 32.967 91 60 1 47 137 71 160 7.90e-11 58.2
MS.gene28429.t1 MTR_4g129370 32.967 91 60 1 47 137 71 160 7.90e-11 58.2
MS.gene28429.t1 MTR_1g096780 36.111 72 44 2 52 122 208 278 9.49e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28429.t1 AT1G49220 60.294 136 50 3 23 155 80 214 1.14e-49 160
MS.gene28429.t1 AT1G49200 55.862 145 60 3 23 164 81 224 1.85e-49 159
MS.gene28429.t1 AT3G18773 53.793 145 64 2 23 164 74 218 5.37e-49 157
MS.gene28429.t1 AT1G49210 61.017 118 43 1 50 164 106 223 1.06e-48 157
MS.gene28429.t1 AT1G49230 53.793 145 60 2 23 163 78 219 1.02e-47 154
MS.gene28429.t1 AT5G05280 54.867 113 41 3 23 135 67 169 4.84e-35 120
MS.gene28429.t1 AT3G10910 48.598 107 50 2 23 125 55 160 3.05e-30 108
MS.gene28429.t1 AT1G20823 47.475 99 48 1 31 125 59 157 2.94e-28 103
MS.gene28429.t1 AT1G76410 46.789 109 55 3 23 130 50 156 3.38e-28 103
MS.gene28429.t1 AT5G01880 44.737 114 52 5 23 135 56 159 1.16e-26 99.0
MS.gene28429.t1 AT2G17450 44.118 102 47 3 38 132 58 156 8.63e-24 92.4
MS.gene28429.t1 AT5G47610 59.677 62 25 0 60 121 88 149 1.25e-22 89.0
MS.gene28429.t1 AT4G35480 41.600 125 53 5 23 132 48 167 7.62e-22 87.8
MS.gene28429.t1 AT1G72200 33.108 148 80 3 7 151 70 201 8.73e-21 87.8
MS.gene28429.t1 AT4G40070 33.117 154 91 4 7 154 53 200 3.47e-20 85.5
MS.gene28429.t1 AT3G05200 35.075 134 64 3 37 149 84 215 8.61e-20 85.1
MS.gene28429.t1 AT2G27940 39.080 87 51 1 46 132 109 193 3.84e-19 81.3
MS.gene28429.t1 AT1G72310 48.684 76 37 1 50 125 100 173 6.14e-19 82.0
MS.gene28429.t1 AT4G17245 57.377 61 25 1 62 121 85 145 9.69e-19 78.6
MS.gene28429.t1 AT1G53820 41.758 91 48 3 50 138 93 180 1.54e-18 80.9
MS.gene28429.t1 AT3G14320 49.296 71 34 1 52 122 63 131 1.92e-18 79.0
MS.gene28429.t1 AT2G25410 36.752 117 72 1 7 121 253 369 5.36e-18 80.1
MS.gene28429.t1 AT3G16720 33.645 107 66 3 49 152 91 195 6.86e-18 79.0
MS.gene28429.t1 AT2G35000 36.607 112 60 3 22 125 73 181 1.15e-17 79.0
MS.gene28429.t1 AT2G20030 39.175 97 56 2 52 146 98 193 1.31e-17 79.0
MS.gene28429.t1 AT5G27420 45.122 82 41 2 48 126 91 171 1.35e-17 79.0
MS.gene28429.t1 AT5G57750 41.096 73 43 0 50 122 93 165 1.41e-17 76.6
MS.gene28429.t1 AT4G30400 43.056 72 41 0 50 121 105 176 1.42e-17 79.3
MS.gene28429.t1 AT4G09120 46.053 76 39 1 51 124 93 168 2.52e-17 77.8
MS.gene28429.t1 AT4G15975 32.090 134 75 3 33 164 32 151 2.60e-17 76.6
MS.gene28429.t1 AT4G09130 44.872 78 41 1 49 124 88 165 4.06e-17 77.4
MS.gene28429.t1 AT4G09100 40.000 80 46 2 45 122 49 128 5.22e-17 73.2
MS.gene28429.t1 AT4G09110 43.590 78 42 1 49 124 91 168 5.24e-17 76.6
MS.gene28429.t1 AT1G22500 34.146 123 73 3 7 125 46 164 5.78e-17 77.0
MS.gene28429.t1 AT4G17905 66.667 45 15 0 77 121 179 223 6.38e-17 76.6
MS.gene28429.t1 AT4G17905 66.667 45 15 0 77 121 153 197 6.44e-17 76.6
MS.gene28429.t1 AT1G35330 42.308 78 43 1 49 124 97 174 6.71e-17 76.6
MS.gene28429.t1 AT2G18650 43.421 76 43 0 50 125 97 172 8.29e-17 77.0
MS.gene28429.t1 AT4G28890 43.662 71 39 1 52 122 97 166 8.72e-17 77.0
MS.gene28429.t1 AT3G03550 59.184 49 20 0 77 125 157 205 8.77e-17 76.6
MS.gene28429.t1 AT5G17600 43.056 72 40 1 50 121 114 184 9.25e-17 76.6
MS.gene28429.t1 AT1G04360 35.455 110 60 3 23 121 67 176 1.20e-16 76.3
MS.gene28429.t1 AT1G74410 34.167 120 71 3 7 124 108 221 1.27e-16 74.3
MS.gene28429.t1 AT1G28040 38.542 96 56 2 27 121 202 295 1.62e-16 75.1
MS.gene28429.t1 AT5G66070 41.558 77 42 2 48 124 148 221 1.27e-15 71.6
MS.gene28429.t1 AT5G66070 41.558 77 42 2 48 124 148 221 1.27e-15 71.6
MS.gene28429.t1 AT4G17920 37.895 95 55 2 34 124 61 155 1.32e-15 72.8
MS.gene28429.t1 AT1G32361 38.235 102 60 2 23 124 101 199 1.39e-15 73.2
MS.gene28429.t1 AT5G05810 41.667 72 41 1 50 121 118 188 1.47e-15 73.2
MS.gene28429.t1 AT2G35420 43.056 72 40 1 50 121 75 145 1.52e-15 72.0
MS.gene28429.t1 AT5G66070 35.294 102 56 3 30 124 147 245 2.26e-15 71.2
MS.gene28429.t1 AT5G10380 40.541 74 43 1 52 125 109 181 2.52e-15 72.0
MS.gene28429.t1 AT3G48030 37.333 75 47 0 50 124 100 174 2.87e-15 71.6
MS.gene28429.t1 AT2G46493 38.750 80 48 1 43 121 101 180 3.06e-15 70.1
MS.gene28429.t1 AT1G72220 57.778 45 19 0 77 121 175 219 3.28e-15 72.4
MS.gene28429.t1 AT2G35910 35.789 95 60 1 52 146 121 214 4.49e-15 70.1
MS.gene28429.t1 AT3G48030 37.333 75 47 0 50 124 178 252 4.54e-15 71.6
MS.gene28429.t1 AT2G34990 33.333 96 62 1 28 121 43 138 5.61e-15 70.9
MS.gene28429.t1 AT2G34990 33.333 96 62 1 28 121 65 160 5.71e-15 71.2
MS.gene28429.t1 AT4G33565 43.662 71 38 1 77 145 215 285 5.86e-15 71.6
MS.gene28429.t1 AT5G42200 37.500 80 49 1 42 121 68 146 8.24e-15 68.2
MS.gene28429.t1 AT3G11110 36.364 121 72 2 1 121 34 149 1.02e-14 68.2
MS.gene28429.t1 AT5G07040 34.568 81 51 2 44 122 57 137 1.23e-14 67.8
MS.gene28429.t1 AT1G23980 38.158 76 46 1 50 125 116 190 1.59e-14 70.1
MS.gene28429.t1 AT4G10160 37.838 74 44 1 52 125 72 143 1.82e-14 68.6
MS.gene28429.t1 AT5G06490 39.474 76 40 1 52 121 96 171 1.89e-14 68.2
MS.gene28429.t1 AT3G62690 35.577 104 55 3 53 154 88 181 1.90e-14 68.9
MS.gene28429.t1 AT5G58580 40.789 76 45 0 50 125 109 184 2.07e-14 69.7
MS.gene28429.t1 AT2G46495 36.082 97 59 2 27 121 267 362 2.20e-14 69.7
MS.gene28429.t1 AT3G19140 42.254 71 39 1 52 122 54 122 2.40e-14 66.6
MS.gene28429.t1 AT2G46495 36.082 97 59 2 27 121 239 334 2.70e-14 69.3
MS.gene28429.t1 AT3G18930 36.047 86 46 2 52 128 120 205 4.07e-14 69.3
MS.gene28429.t1 AT3G18930 36.047 86 46 2 52 128 120 205 4.07e-14 69.3
MS.gene28429.t1 AT3G18930 36.047 86 46 2 52 128 120 205 4.07e-14 69.3
MS.gene28429.t1 AT2G46160 34.043 94 55 1 41 127 93 186 4.10e-14 67.4
MS.gene28429.t1 AT2G46160 34.043 94 55 1 41 127 93 186 4.10e-14 67.4
MS.gene28429.t1 AT5G53110 39.437 71 42 1 52 121 302 372 5.49e-14 68.9
MS.gene28429.t1 AT4G10150 36.486 74 45 1 52 125 86 157 7.09e-14 67.4
MS.gene28429.t1 AT2G46494 32.292 96 64 1 27 121 263 358 7.57e-14 68.2
MS.gene28429.t1 AT3G61550 31.250 144 73 5 27 160 80 207 7.80e-14 66.6
MS.gene28429.t1 AT1G33480 30.189 106 69 3 48 152 71 172 7.99e-14 67.4
MS.gene28429.t1 AT2G34000 36.559 93 55 2 51 140 60 151 1.54e-13 64.7
MS.gene28429.t1 AT5G40250 50.000 48 24 0 78 125 142 189 1.67e-13 67.4
MS.gene28429.t1 AT5G36001 59.574 47 19 0 76 122 261 307 1.87e-13 67.0
MS.gene28429.t1 AT4G35840 38.750 80 41 2 50 124 160 236 2.70e-13 65.9
MS.gene28429.t1 AT2G47560 41.096 73 42 1 50 121 78 150 2.82e-13 65.5
MS.gene28429.t1 AT2G18670 34.667 75 49 0 47 121 78 152 2.83e-13 64.7
MS.gene28429.t1 AT1G35630 35.714 98 61 2 55 151 209 305 4.45e-13 65.9
MS.gene28429.t1 AT5G43420 56.818 44 19 0 78 121 137 180 5.51e-13 65.9
MS.gene28429.t1 AT2G17730 54.545 44 20 0 79 122 196 239 6.60e-13 64.7
MS.gene28429.t1 AT1G35625 34.694 98 62 2 55 151 209 305 2.24e-12 63.9
MS.gene28429.t1 AT2G42360 38.028 71 44 0 51 121 80 150 2.75e-12 62.8
MS.gene28429.t1 AT4G26400 44.118 68 30 2 54 121 223 282 2.99e-12 63.5
MS.gene28429.t1 AT4G26400 44.118 68 30 2 54 121 223 282 2.99e-12 63.5
MS.gene28429.t1 AT2G42350 37.333 75 45 1 47 121 70 142 3.44e-12 62.4
MS.gene28429.t1 AT4G26580 35.484 93 54 2 37 124 242 333 6.88e-12 62.8
MS.gene28429.t1 AT4G26580 35.484 93 54 2 37 124 242 333 6.88e-12 62.8
MS.gene28429.t1 AT1G53010 50.000 50 25 0 78 127 129 178 8.37e-12 60.8
MS.gene28429.t1 AT5G56340 44.118 68 30 2 54 121 241 300 9.70e-12 62.4
MS.gene28429.t1 AT5G52150 37.500 96 52 3 26 114 104 198 1.36e-11 60.5
MS.gene28429.t1 AT2G37580 48.936 47 24 0 75 121 138 184 1.72e-11 60.8
MS.gene28429.t1 AT3G47990 52.000 50 23 1 72 121 171 219 1.92e-11 61.2
MS.gene28429.t1 AT3G47990 52.000 50 23 1 72 121 171 219 1.92e-11 61.2
MS.gene28429.t1 AT3G47990 52.000 50 23 1 72 121 171 219 1.92e-11 61.2
MS.gene28429.t1 AT3G47990 52.000 50 23 1 72 121 228 276 1.97e-11 61.2
MS.gene28429.t1 AT3G47990 52.000 50 23 1 72 121 212 260 2.19e-11 61.2
MS.gene28429.t1 AT3G20395 30.000 100 67 1 25 124 120 216 2.86e-11 60.1
MS.gene28429.t1 AT5G46650 34.091 88 55 2 40 124 73 160 2.87e-11 60.5
MS.gene28429.t1 AT4G11360 35.484 93 55 3 35 125 44 133 3.31e-11 58.5
MS.gene28429.t1 AT2G03000 51.064 47 22 1 78 124 480 525 4.42e-11 60.5
MS.gene28429.t1 AT3G61180 35.870 92 56 2 47 136 289 379 5.06e-11 60.1
MS.gene28429.t1 AT1G12760 34.066 91 54 2 37 127 245 329 6.17e-11 60.1
MS.gene28429.t1 AT4G30370 35.616 73 46 1 50 122 87 158 6.27e-11 58.2
MS.gene28429.t1 AT3G60966 55.814 43 19 0 79 121 62 104 6.55e-11 57.4
MS.gene28429.t1 AT1G12760 34.066 91 54 2 37 127 316 400 6.72e-11 60.1
MS.gene28429.t1 AT4G35840 36.957 92 51 2 40 124 177 268 8.21e-11 59.3

Find 36 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGTTGAAACTTCCAAATT+TGG 0.155862 4.4:-8790672 MS.gene28429:CDS
TGAAATCCATGGTTGCATTT+AGG 0.236983 4.4:+8790590 None:intergenic
ACACACTCTGTATCCAAATT+TGG 0.282885 4.4:+8790659 None:intergenic
TGGATCGTTATAATAGTTTC+TGG 0.322018 4.4:+8790446 None:intergenic
CTTGAAACTTGTCAGAAGAT+TGG 0.343028 4.4:-8790503 MS.gene28429:CDS
TAGTTACGCTCCAAAGCTTC+AGG 0.368913 4.4:+8790413 None:intergenic
ACTAGAAGAGTCGTTGTTGA+TGG 0.380702 4.4:+8790781 None:intergenic
AAGCTTTGGAGCGTAACTAT+AGG 0.382409 4.4:-8790409 MS.gene28429:CDS
ACCACTAGAACCTGAAGCTT+TGG 0.391611 4.4:-8790423 MS.gene28429:CDS
CCATTATTAGGTGTGCCTTA+AGG 0.397999 4.4:-8790817 MS.gene28429:intron
TCTGGCACTGGCAACACAAT+TGG 0.406141 4.4:+8790464 None:intergenic
ACGTTCGATGCATTGACAAA+TGG 0.439104 4.4:-8790568 MS.gene28429:CDS
AGCTTTGGAGCGTAACTATA+GGG 0.446064 4.4:-8790408 MS.gene28429:CDS
CAGCAATCCTTCACCTCAAT+CGG 0.461316 4.4:-8790753 MS.gene28429:CDS
GTTATAATAGTTTCTGGCAC+TGG 0.477505 4.4:+8790452 None:intergenic
AAGCTTCAGGTTCTAGTGGT+TGG 0.485771 4.4:+8790426 None:intergenic
TTGTTGACCGATTGAGGTGA+AGG 0.495226 4.4:+8790746 None:intergenic
ATTGAATAGTAGTGAAGTAA+AGG 0.495792 4.4:+8791039 None:intergenic
GGCTACGTTTGAAAACCTTA+AGG 0.504858 4.4:+8790802 None:intergenic
ATTCCTTTGTTGACCGATTG+AGG 0.506390 4.4:+8790740 None:intergenic
TTGTGGAATCTCACACAAGA+AGG 0.511006 4.4:-8791009 MS.gene28429:intron
TCAGAAGATTGGTGGGTCGC+AGG 0.526432 4.4:-8790492 MS.gene28429:CDS
AAGAAGGCACTGTCTACACT+TGG 0.527278 4.4:+8790523 None:intergenic
GAAACTTGTCAGAAGATTGG+TGG 0.537375 4.4:-8790500 MS.gene28429:CDS
ATGCATTGACAAATGGCTAA+AGG 0.546565 4.4:-8790561 MS.gene28429:CDS
ACTATTCAATGAGCTTCTTG+TGG 0.547062 4.4:-8791026 MS.gene28429:CDS
TCTTCTGACAAGTTTCAAGA+AGG 0.569705 4.4:+8790507 None:intergenic
TGTCTCTCAGAGTTCACAAA+GGG 0.586259 4.4:-8790632 MS.gene28429:CDS
TCCAAAGCTTCAGGTTCTAG+TGG 0.589399 4.4:+8790422 None:intergenic
AAACTTGTCAGAAGATTGGT+GGG 0.594232 4.4:-8790499 MS.gene28429:CDS
TGCATCGAACGTGAAATCCA+TGG 0.600928 4.4:+8790579 None:intergenic
TCAGTTGAGTAGCTCACTGT+GGG 0.618570 4.4:+8790692 None:intergenic
TCACCTCAATCGGTCAACAA+AGG 0.632009 4.4:-8790743 MS.gene28429:CDS
AGAAGGCACTGTCTACACTT+GGG 0.655706 4.4:+8790524 None:intergenic
CTCAGTTGAGTAGCTCACTG+TGG 0.674782 4.4:+8790691 None:intergenic
ATGTCTCTCAGAGTTCACAA+AGG 0.686490 4.4:-8790633 MS.gene28429:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATGAGTCTGTTGAATTA+TGG + chr4.4:8790534-8790553 None:intergenic 25.0%
! ATTGAATAGTAGTGAAGTAA+AGG + chr4.4:8790419-8790438 None:intergenic 25.0%
! TTGCATTAAACTCCATTATT+AGG - chr4.4:8790626-8790645 MS.gene28429:CDS 25.0%
!! AATTCAACAGACTCATATTT+TGG - chr4.4:8790535-8790554 MS.gene28429:CDS 25.0%
!!! TGTTGAATTATGGTTTTTTG+TGG + chr4.4:8790524-8790543 None:intergenic 25.0%
TGGATCGTTATAATAGTTTC+TGG + chr4.4:8791012-8791031 None:intergenic 30.0%
! ATTTTACCTAAATGCAACCA+TGG - chr4.4:8790859-8790878 MS.gene28429:intron 30.0%
! TGAGTTGAAACTTCCAAATT+TGG - chr4.4:8790783-8790802 MS.gene28429:CDS 30.0%
! TTAGTGATTGATGATCAACT+GGG + chr4.4:8790486-8790505 None:intergenic 30.0%
!!! TATGGTTTTTTGTGGAAACA+GGG + chr4.4:8790516-8790535 None:intergenic 30.0%
!!! TTATGGTTTTTTGTGGAAAC+AGG + chr4.4:8790517-8790536 None:intergenic 30.0%
AAACTTGTCAGAAGATTGGT+GGG - chr4.4:8790956-8790975 MS.gene28429:intron 35.0%
ACACACTCTGTATCCAAATT+TGG + chr4.4:8790799-8790818 None:intergenic 35.0%
ACTATTCAATGAGCTTCTTG+TGG - chr4.4:8790429-8790448 MS.gene28429:CDS 35.0%
CTTGAAACTTGTCAGAAGAT+TGG - chr4.4:8790952-8790971 MS.gene28429:intron 35.0%
TCTTCTGACAAGTTTCAAGA+AGG + chr4.4:8790951-8790970 None:intergenic 35.0%
TGAAATCCATGGTTGCATTT+AGG + chr4.4:8790868-8790887 None:intergenic 35.0%
! ATGCATTGACAAATGGCTAA+AGG - chr4.4:8790894-8790913 MS.gene28429:intron 35.0%
! GTTAGTGATTGATGATCAAC+TGG + chr4.4:8790487-8790506 None:intergenic 35.0%
!! GTTATAATAGTTTCTGGCAC+TGG + chr4.4:8791006-8791025 None:intergenic 35.0%
!!! ATGGTTTTTTGTGGAAACAG+GGG + chr4.4:8790515-8790534 None:intergenic 35.0%
ACTAGAAGAGTCGTTGTTGA+TGG + chr4.4:8790677-8790696 None:intergenic 40.0%
ATGTCTCTCAGAGTTCACAA+AGG - chr4.4:8790822-8790841 MS.gene28429:intron 40.0%
ATTCCTTTGTTGACCGATTG+AGG + chr4.4:8790718-8790737 None:intergenic 40.0%
CCATTATTAGGTGTGCCTTA+AGG - chr4.4:8790638-8790657 MS.gene28429:CDS 40.0%
CCTTAAGGCACACCTAATAA+TGG + chr4.4:8790641-8790660 None:intergenic 40.0%
GAAACTTGTCAGAAGATTGG+TGG - chr4.4:8790955-8790974 MS.gene28429:intron 40.0%
GGCTACGTTTGAAAACCTTA+AGG + chr4.4:8790656-8790675 None:intergenic 40.0%
TGTCTCTCAGAGTTCACAAA+GGG - chr4.4:8790823-8790842 MS.gene28429:intron 40.0%
TTGTGGAATCTCACACAAGA+AGG - chr4.4:8790446-8790465 MS.gene28429:CDS 40.0%
! ACGTTCGATGCATTGACAAA+TGG - chr4.4:8790887-8790906 MS.gene28429:intron 40.0%
!! AAGCTTTGGAGCGTAACTAT+AGG - chr4.4:8791046-8791065 MS.gene28429:CDS 40.0%
!! AGCTTTGGAGCGTAACTATA+GGG - chr4.4:8791047-8791066 MS.gene28429:CDS 40.0%
ACCACTAGAACCTGAAGCTT+TGG - chr4.4:8791032-8791051 MS.gene28429:CDS 45.0%
CAGCAATCCTTCACCTCAAT+CGG - chr4.4:8790702-8790721 MS.gene28429:CDS 45.0%
TAGTTACGCTCCAAAGCTTC+AGG + chr4.4:8791045-8791064 None:intergenic 45.0%
TCACCTCAATCGGTCAACAA+AGG - chr4.4:8790712-8790731 MS.gene28429:CDS 45.0%
TCAGTTGAGTAGCTCACTGT+GGG + chr4.4:8790766-8790785 None:intergenic 45.0%
TGCATCGAACGTGAAATCCA+TGG + chr4.4:8790879-8790898 None:intergenic 45.0%
TTGTTGACCGATTGAGGTGA+AGG + chr4.4:8790712-8790731 None:intergenic 45.0%
! AAGCTTCAGGTTCTAGTGGT+TGG + chr4.4:8791032-8791051 None:intergenic 45.0%
! TCCAAAGCTTCAGGTTCTAG+TGG + chr4.4:8791036-8791055 None:intergenic 45.0%
! TTTTGTGGAAACAGGGGAGT+TGG + chr4.4:8790509-8790528 None:intergenic 45.0%
!! AAGAAGGCACTGTCTACACT+TGG + chr4.4:8790935-8790954 None:intergenic 45.0%
!! AGAAGGCACTGTCTACACTT+GGG + chr4.4:8790934-8790953 None:intergenic 45.0%
CTCAGTTGAGTAGCTCACTG+TGG + chr4.4:8790767-8790786 None:intergenic 50.0%
!! TCTGGCACTGGCAACACAAT+TGG + chr4.4:8790994-8791013 None:intergenic 50.0%
! TCAGAAGATTGGTGGGTCGC+AGG - chr4.4:8790963-8790982 MS.gene28429:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 8790400 8791077 8790400 ID=MS.gene28429
chr4.4 mRNA 8790400 8791077 8790400 ID=MS.gene28429.t1;Parent=MS.gene28429
chr4.4 exon 8791010 8791077 8791010 ID=MS.gene28429.t1.exon1;Parent=MS.gene28429.t1
chr4.4 CDS 8791010 8791077 8791010 ID=cds.MS.gene28429.t1;Parent=MS.gene28429.t1
chr4.4 exon 8790400 8790829 8790400 ID=MS.gene28429.t1.exon2;Parent=MS.gene28429.t1
chr4.4 CDS 8790400 8790829 8790400 ID=cds.MS.gene28429.t1;Parent=MS.gene28429.t1
Gene Sequence

>MS.gene28429

ATGTATGCTTCTACTTCCTTTACTTCACTACTATTCAATGAGCTTCTTGTGGAATCTCACACAAGAAGGTTACTCATTCAAAACCCAGTTGATCATCAATCACTAACCAACTCCCCTGTTTCCACAAAAAACCATAATTCAACAGACTCATATTTTGGAGCTTGTGAATTTGATTCAAAAGTTGTGATGATACTTGCAGTCATATTATGTGTTTTTATTTGCTCACTTGCATTAAACTCCATTATTAGGTGTGCCTTAAGGTTTTCAAACGTAGCCATCAACAACGACTCTTCTAGTTCAAACAGCAATCCTTCACCTCAATCGGTCAACAAAGGAATCAAGAAGAAAGCTCTCAAGACATTTCCCACAGTGAGCTACTCAACTGAGTTGAAACTTCCAAATTTGGATACAGAGTGTGTGATATGTCTCTCAGAGTTCACAAAGGGTGAAAAGATACGCATTTTACCTAAATGCAACCATGGATTTCACGTTCGATGCATTGACAAATGGCTAAAGGAACACTCATCATGTCCCAAGTGTAGACAGTGCCTTCTTGAAACTTGTCAGAAGATTGGTGGGTCGCAGGTGCAACCAATTGTGTTGCCAGTGCCAGAAACTATTATAACGATCCAACCACTAGAACCTGAAGCTTTGGAGCGTAACTATAGGGAAATATAA
Protein sequence

>MS.gene28429.t1

MYASTSFTSLLFNELLVESHTRRCALRFSNVAINNDSSSSNSNPSPQSVNKGIKKKALKTFPTVSYSTELKLPNLDTECVICLSEFTKGEKIRILPKCNHGFHVRCIDKWLKEHSSCPKCRQCLLETCQKIGGSQVQPIVLPVPETIITIQPLEPEALERNYREI