AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28430.t1 MTR_8g092840 91.403 221 19 0 1 221 1 221 2.53e-136 387
MS.gene28430.t1 MTR_8g092960 81.900 221 30 2 1 221 1 211 1.52e-119 344
MS.gene28430.t1 MTR_8g093010 78.636 220 37 3 2 220 3 213 5.79e-114 330
MS.gene28430.t1 MTR_8g092970 77.727 220 40 1 1 220 1 211 2.77e-108 316
MS.gene28430.t1 MTR_8g092930 77.273 220 49 1 1 220 1 219 2.78e-106 311
MS.gene28430.t1 MTR_8g092990 80.995 221 41 1 1 220 1 221 4.11e-105 308
MS.gene28430.t1 MTR_8g092985 76.056 213 47 2 1 212 1 210 5.85e-100 294
MS.gene28430.t1 MTR_3g095560 58.233 249 82 7 1 240 1 236 1.97e-83 253
MS.gene28430.t1 MTR_3g094000 52.273 132 57 2 57 187 40 166 3.20e-37 132
MS.gene28430.t1 MTR_7g073200 48.252 143 66 3 45 186 18 153 5.29e-37 132
MS.gene28430.t1 MTR_3g116000 48.344 151 61 6 56 200 24 163 5.00e-33 122
MS.gene28430.t1 MTR_7g033625 73.494 83 7 1 217 284 16 98 6.29e-33 125
MS.gene28430.t1 MTR_5g024730 48.571 140 58 5 56 191 28 157 2.01e-32 120
MS.gene28430.t1 MTR_2g035800 38.961 154 82 3 30 183 4 145 7.74e-30 112
MS.gene28430.t1 MTR_8g073120 48.062 129 54 4 57 182 22 140 3.49e-29 112
MS.gene28430.t1 MTR_4g094725 38.158 152 84 3 33 183 7 149 2.77e-27 105
MS.gene28430.t1 MTR_6g004780 59.036 83 19 1 217 284 24 106 2.63e-22 96.7
MS.gene28430.t1 MTR_7g113800 34.868 152 77 5 44 186 28 166 1.23e-19 89.4
MS.gene28430.t1 MTR_3g110190 37.209 129 74 3 66 188 13 140 1.98e-19 88.6
MS.gene28430.t1 MTR_1g082550 32.680 153 84 4 42 186 32 173 5.03e-19 87.4
MS.gene28430.t1 MTR_1g043410 28.000 150 93 3 43 183 23 166 6.66e-19 86.7
MS.gene28430.t1 MTR_7g117540 31.818 132 88 1 57 188 20 149 5.08e-18 83.6
MS.gene28430.t1 MTR_8g023400 45.570 79 40 2 113 190 92 168 6.16e-18 84.0
MS.gene28430.t1 MTR_1g100737 38.636 132 69 2 62 189 49 172 6.21e-18 81.3
MS.gene28430.t1 MTR_6g055030 33.951 162 88 5 24 182 23 168 8.99e-18 83.2
MS.gene28430.t1 MTR_4g120270 43.836 73 38 1 110 182 77 146 2.60e-17 81.3
MS.gene28430.t1 MTR_3g071300 31.013 158 95 3 57 211 23 169 3.87e-17 78.6
MS.gene28430.t1 MTR_5g064980 31.507 146 87 3 42 185 48 182 5.79e-17 81.6
MS.gene28430.t1 MTR_6g087150 31.915 188 106 5 30 211 19 190 7.30e-17 81.3
MS.gene28430.t1 MTR_3g078450 33.974 156 92 4 88 240 51 198 7.32e-17 79.0
MS.gene28430.t1 MTR_5g082940 46.053 76 40 1 111 185 73 148 1.14e-16 77.0
MS.gene28430.t1 MTR_7g116220 40.000 80 46 1 110 187 67 146 1.78e-16 77.0
MS.gene28430.t1 MTR_4g063070 40.698 86 50 1 103 187 298 383 1.92e-16 80.1
MS.gene28430.t1 MTR_3g099650 43.590 78 42 1 111 186 105 182 2.36e-16 79.7
MS.gene28430.t1 MTR_4g056590 41.250 80 42 2 110 189 97 171 2.42e-16 77.0
MS.gene28430.t1 MTR_8g066780 28.369 282 133 10 26 279 44 284 3.23e-16 79.3
MS.gene28430.t1 MTR_2g096350 60.417 48 19 0 135 182 130 177 5.88e-16 76.3
MS.gene28430.t1 MTR_5g071930 48.684 76 35 2 112 187 65 136 8.16e-16 75.5
MS.gene28430.t1 MTR_4g030070 29.839 124 82 2 103 226 75 193 8.32e-16 76.3
MS.gene28430.t1 MTR_1g492830 27.933 179 110 3 46 224 7 166 1.05e-15 75.9
MS.gene28430.t1 MTR_3g078470 37.374 99 61 1 88 185 51 149 1.52e-15 75.1
MS.gene28430.t1 MTR_4g129420 43.038 79 45 0 108 186 112 190 1.65e-15 77.8
MS.gene28430.t1 MTR_3g464780 42.308 78 42 1 112 186 101 178 2.14e-15 75.5
MS.gene28430.t1 MTR_2g436100 40.506 79 46 1 104 182 84 161 3.72e-15 75.5
MS.gene28430.t1 MTR_5g064890 29.655 145 93 4 46 182 53 196 4.00e-15 75.9
MS.gene28430.t1 MTR_6g004740 77.500 40 9 0 219 258 2 41 4.19e-15 71.6
MS.gene28430.t1 MTR_7g076250 41.429 70 41 0 113 182 63 132 4.22e-15 73.2
MS.gene28430.t1 MTR_8g089880 37.500 80 49 1 113 192 127 205 1.12e-14 73.6
MS.gene28430.t1 MTR_7g116180 38.667 75 44 1 113 185 67 141 1.48e-14 71.6
MS.gene28430.t1 MTR_3g100400 43.056 72 40 1 111 182 128 198 1.74e-14 74.3
MS.gene28430.t1 MTR_2g055790 36.697 109 65 1 78 186 42 146 2.00e-14 71.6
MS.gene28430.t1 MTR_1g070425 38.667 75 44 1 113 185 68 142 2.17e-14 70.9
MS.gene28430.t1 MTR_5g030010 41.333 75 43 1 112 186 121 194 2.51e-14 73.9
MS.gene28430.t1 MTR_1g070140 39.744 78 45 2 106 182 96 172 3.53e-14 73.2
MS.gene28430.t1 MTR_4g093830 42.857 70 39 1 113 182 126 194 3.81e-14 73.2
MS.gene28430.t1 MTR_5g026730 30.597 134 86 3 53 182 6 136 4.74e-14 70.1
MS.gene28430.t1 MTR_2g009310 60.000 45 18 0 138 182 159 203 4.89e-14 73.2
MS.gene28430.t1 MTR_3g012550 27.068 133 94 3 52 183 21 151 5.89e-14 69.7
MS.gene28430.t1 MTR_5g064990 39.744 78 45 1 111 186 112 189 8.43e-14 72.4
MS.gene28430.t1 MTR_3g091620 41.558 77 44 1 110 186 98 173 9.96e-14 72.0
MS.gene28430.t1 MTR_7g108855 38.298 94 48 3 114 198 67 159 1.05e-13 69.3
MS.gene28430.t1 MTR_4g106680 28.235 170 100 3 34 182 6 174 1.23e-13 71.2
MS.gene28430.t1 MTR_6g007737 30.370 135 83 3 61 185 71 204 1.77e-13 71.2
MS.gene28430.t1 MTR_2g090905 41.558 77 40 1 111 182 37 113 1.96e-13 67.0
MS.gene28430.t1 MTR_3g086700 36.957 92 51 2 98 182 26 117 2.01e-13 68.6
MS.gene28430.t1 MTR_5g009190 37.805 82 50 1 103 183 256 337 2.34e-13 70.5
MS.gene28430.t1 MTR_4g108830 35.354 99 60 2 86 183 122 217 2.61e-13 68.9
MS.gene28430.t1 MTR_5g066250 40.278 72 42 1 111 182 132 202 2.62e-13 70.9
MS.gene28430.t1 MTR_2g090950 29.078 141 95 1 47 182 25 165 2.74e-13 69.3
MS.gene28430.t1 MTR_5g009210 38.272 81 49 1 104 183 278 358 3.21e-13 70.5
MS.gene28430.t1 MTR_1g074190 34.409 93 52 2 105 190 91 181 3.28e-13 70.1
MS.gene28430.t1 MTR_5g034790 40.260 77 45 1 109 185 112 187 3.36e-13 70.5
MS.gene28430.t1 MTR_3g086730 39.241 79 46 1 109 185 42 120 3.48e-13 68.9
MS.gene28430.t1 MTR_3g007930 35.156 128 50 3 220 314 72 199 3.99e-13 69.7
MS.gene28430.t1 MTR_5g096090 35.156 128 50 3 220 314 72 199 4.10e-13 69.7
MS.gene28430.t1 MTR_8g028465 38.028 71 41 1 113 183 90 157 4.19e-13 67.8
MS.gene28430.t1 MTR_3g114500 32.727 110 68 3 75 182 8 113 4.39e-13 65.9
MS.gene28430.t1 MTR_5g096090 38.679 106 40 2 220 300 72 177 5.43e-13 70.1
MS.gene28430.t1 MTR_1g056350 37.179 78 45 2 108 182 69 145 5.89e-13 66.6
MS.gene28430.t1 MTR_3g007930 38.679 106 40 2 220 300 72 177 6.17e-13 69.7
MS.gene28430.t1 MTR_2g041200 31.507 146 76 5 46 182 21 151 6.38e-13 67.0
MS.gene28430.t1 MTR_8g099495 38.462 78 47 1 107 183 313 390 8.08e-13 69.3
MS.gene28430.t1 MTR_5g092790 43.836 73 38 2 112 182 156 227 8.85e-13 67.8
MS.gene28430.t1 MTR_5g026720 30.252 119 74 3 72 182 18 135 1.01e-12 65.9
MS.gene28430.t1 MTR_8g074900 41.333 75 39 1 111 185 156 225 1.09e-12 67.0
MS.gene28430.t1 MTR_8g063660 32.090 134 83 3 61 187 9 141 1.16e-12 68.6
MS.gene28430.t1 MTR_6g007670 34.694 98 51 3 109 203 1 88 1.22e-12 67.4
MS.gene28430.t1 MTR_4g108830 35.052 97 59 2 88 183 96 189 1.52e-12 65.9
MS.gene28430.t1 MTR_7g079290 42.623 61 35 0 137 197 152 212 1.57e-12 66.6
MS.gene28430.t1 MTR_3g091200 39.437 71 42 1 113 182 303 373 2.00e-12 68.2
MS.gene28430.t1 MTR_5g009200 40.845 71 41 1 113 182 322 392 2.40e-12 67.8
MS.gene28430.t1 MTR_5g076990 32.558 129 81 3 59 182 21 148 3.07e-12 67.0
MS.gene28430.t1 MTR_1g026070 36.486 74 43 1 109 182 44 113 3.13e-12 63.5
MS.gene28430.t1 MTR_7g059360 45.455 66 35 1 119 183 136 201 3.37e-12 65.9
MS.gene28430.t1 MTR_7g079280 36.364 77 48 1 113 188 80 156 3.92e-12 64.7
MS.gene28430.t1 MTR_8g032690 48.077 52 27 0 140 191 19 70 3.96e-12 62.8
MS.gene28430.t1 MTR_5g022920 37.838 74 45 1 111 183 159 232 5.53e-12 65.1
MS.gene28430.t1 MTR_6g007680 40.845 71 41 1 112 182 39 108 6.17e-12 63.5
MS.gene28430.t1 MTR_8g019710 57.778 45 19 0 138 182 52 96 6.78e-12 62.8
MS.gene28430.t1 MTR_7g116200 39.437 71 41 1 112 182 67 135 8.63e-12 63.5
MS.gene28430.t1 MTR_5g092750 45.205 73 37 2 112 182 156 227 1.09e-11 64.3
MS.gene28430.t1 MTR_8g009190 28.467 137 87 3 57 186 19 151 1.13e-11 65.5
MS.gene28430.t1 MTR_4g022410 30.882 136 87 3 63 198 43 171 1.14e-11 63.5
MS.gene28430.t1 MTR_3g078120 31.200 125 81 4 61 182 5 127 2.01e-11 62.0
MS.gene28430.t1 MTR_8g071815 53.488 43 20 0 140 182 90 132 2.79e-11 62.0
MS.gene28430.t1 MTR_8g099515 40.278 72 42 1 113 183 309 380 3.15e-11 64.7
MS.gene28430.t1 MTR_2g079320 32.432 111 66 2 82 185 96 204 3.29e-11 62.4
MS.gene28430.t1 MTR_3g087900 47.692 65 29 3 123 186 55 115 3.96e-11 61.2
MS.gene28430.t1 MTR_3g081030 34.146 82 52 1 117 198 98 177 5.92e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28430.t1 AT1G49230 50.230 217 81 5 11 219 17 214 2.97e-61 196
MS.gene28430.t1 AT1G49220 47.087 206 86 4 21 215 23 216 6.49e-57 186
MS.gene28430.t1 AT1G49200 45.631 206 92 4 21 218 24 217 1.76e-55 181
MS.gene28430.t1 AT1G49210 47.573 206 88 4 21 218 23 216 8.02e-55 179
MS.gene28430.t1 AT3G18773 46.535 202 90 6 21 218 24 211 3.26e-50 167
MS.gene28430.t1 AT5G05280 52.113 142 61 2 46 187 26 160 8.74e-42 144
MS.gene28430.t1 AT3G10910 48.077 156 64 4 49 201 26 167 4.29e-38 134
MS.gene28430.t1 AT1G20823 43.452 168 78 6 23 186 3 157 2.27e-34 125
MS.gene28430.t1 AT1G76410 43.709 151 76 2 41 188 8 152 1.12e-32 120
MS.gene28430.t1 AT2G17450 41.176 136 59 4 58 185 25 147 1.11e-26 105
MS.gene28430.t1 AT5G01880 40.860 186 82 6 12 196 1 159 1.39e-26 103
MS.gene28430.t1 AT5G47610 35.625 160 91 3 23 182 2 149 1.17e-24 99.0
MS.gene28430.t1 AT4G35480 36.264 182 83 5 54 217 19 185 1.27e-24 99.8
MS.gene28430.t1 AT4G17245 41.270 126 57 3 59 182 35 145 1.88e-22 92.8
MS.gene28430.t1 AT1G22500 35.664 143 83 4 59 198 40 176 8.90e-19 87.0
MS.gene28430.t1 AT4G40070 34.000 150 90 3 64 209 49 193 1.16e-18 85.9
MS.gene28430.t1 AT1G72310 48.718 78 38 1 109 186 98 173 7.28e-18 83.6
MS.gene28430.t1 AT3G05200 32.394 142 92 2 48 187 36 175 1.59e-17 83.6
MS.gene28430.t1 AT1G72200 33.083 133 85 3 57 186 59 190 2.54e-17 82.8
MS.gene28430.t1 AT1G28040 37.143 105 63 2 82 183 192 296 5.57e-17 80.9
MS.gene28430.t1 AT3G14320 47.887 71 35 1 113 183 63 131 8.65e-17 78.6
MS.gene28430.t1 AT2G25410 32.716 162 102 2 23 182 213 369 1.01e-16 80.9
MS.gene28430.t1 AT1G53820 44.872 78 39 2 109 185 91 165 1.25e-16 80.1
MS.gene28430.t1 AT5G57750 32.283 127 81 2 61 183 40 165 1.44e-16 77.8
MS.gene28430.t1 AT2G27940 36.634 101 61 2 99 199 101 198 1.45e-16 78.6
MS.gene28430.t1 AT2G35000 32.331 133 86 2 58 186 49 181 2.83e-16 79.7
MS.gene28430.t1 AT1G35330 29.762 168 98 4 24 185 21 174 3.17e-16 79.0
MS.gene28430.t1 AT4G09110 33.065 124 75 2 64 185 51 168 4.69e-16 78.2
MS.gene28430.t1 AT5G27420 40.566 106 50 4 110 212 92 187 5.37e-16 78.6
MS.gene28430.t1 AT3G16720 39.326 89 51 2 109 197 90 175 6.44e-16 77.8
MS.gene28430.t1 AT1G04360 30.303 132 82 2 61 182 45 176 8.47e-16 78.2
MS.gene28430.t1 AT4G10160 33.071 127 75 3 63 186 24 143 1.06e-15 75.9
MS.gene28430.t1 AT1G74410 38.462 91 55 1 95 185 132 221 1.31e-15 75.5
MS.gene28430.t1 AT4G09120 32.857 140 83 3 48 185 38 168 1.69e-15 77.0
MS.gene28430.t1 AT4G15975 37.363 91 55 1 95 185 33 121 1.75e-15 75.5
MS.gene28430.t1 AT2G20030 33.884 121 77 3 113 232 98 216 2.26e-15 77.0
MS.gene28430.t1 AT2G18650 43.590 78 44 0 109 186 95 172 2.30e-15 77.4
MS.gene28430.t1 AT4G17905 66.667 45 15 0 138 182 153 197 2.35e-15 76.3
MS.gene28430.t1 AT1G23980 30.000 180 98 7 30 186 16 190 2.41e-15 76.6
MS.gene28430.t1 AT4G17905 66.667 45 15 0 138 182 179 223 2.43e-15 76.6
MS.gene28430.t1 AT3G03550 59.184 49 20 0 138 186 157 205 2.96e-15 76.6
MS.gene28430.t1 AT5G17600 33.333 129 75 3 62 182 59 184 3.55e-15 76.3
MS.gene28430.t1 AT4G30400 41.333 75 44 0 108 182 102 176 3.93e-15 76.6
MS.gene28430.t1 AT4G09100 39.241 79 46 2 106 182 49 127 9.95e-15 70.9
MS.gene28430.t1 AT4G09130 43.590 78 42 1 110 185 88 165 1.22e-14 74.7
MS.gene28430.t1 AT2G46493 33.663 101 64 2 85 182 80 180 1.42e-14 71.6
MS.gene28430.t1 AT1G33480 31.193 109 72 2 105 213 67 172 1.51e-14 73.2
MS.gene28430.t1 AT4G28890 44.286 70 38 1 113 182 97 165 1.54e-14 74.7
MS.gene28430.t1 AT2G46494 30.952 126 81 3 64 183 234 359 2.47e-14 73.9
MS.gene28430.t1 AT5G05810 43.056 72 40 1 111 182 118 188 3.69e-14 73.6
MS.gene28430.t1 AT2G34990 29.008 131 91 1 54 182 8 138 7.62e-14 71.6
MS.gene28430.t1 AT5G07040 27.612 134 84 3 52 183 15 137 8.03e-14 68.9
MS.gene28430.t1 AT1G72220 57.778 45 19 0 138 182 175 219 8.14e-14 72.4
MS.gene28430.t1 AT5G66070 43.421 76 40 2 110 185 149 221 1.09e-13 70.1
MS.gene28430.t1 AT5G66070 43.421 76 40 2 110 185 149 221 1.09e-13 70.1
MS.gene28430.t1 AT2G34990 29.008 131 91 1 54 182 30 160 1.12e-13 71.6
MS.gene28430.t1 AT1G32361 37.500 104 59 4 83 185 101 199 1.15e-13 71.6
MS.gene28430.t1 AT2G35420 43.056 72 40 1 111 182 75 145 1.19e-13 70.5
MS.gene28430.t1 AT3G62690 30.496 141 95 3 44 182 16 155 1.20e-13 70.5
MS.gene28430.t1 AT5G10380 39.241 79 47 1 108 186 104 181 1.21e-13 71.2
MS.gene28430.t1 AT3G48030 36.782 87 49 1 105 185 88 174 1.53e-13 70.5
MS.gene28430.t1 AT5G66070 36.893 103 56 3 89 185 146 245 1.56e-13 70.1
MS.gene28430.t1 AT3G48030 36.782 87 49 1 105 185 166 252 2.00e-13 70.9
MS.gene28430.t1 AT2G35910 39.474 76 45 1 113 188 121 195 2.00e-13 69.3
MS.gene28430.t1 AT5G06490 39.474 76 40 1 113 182 96 171 2.04e-13 68.6
MS.gene28430.t1 AT5G42200 39.189 74 44 1 109 182 74 146 2.36e-13 67.8
MS.gene28430.t1 AT4G33565 52.941 51 24 0 138 188 215 265 2.40e-13 70.9
MS.gene28430.t1 AT2G42360 28.571 140 87 2 46 182 21 150 2.64e-13 69.3
MS.gene28430.t1 AT3G18930 35.652 115 59 4 113 217 120 229 4.05e-13 70.5
MS.gene28430.t1 AT3G18930 35.652 115 59 4 113 217 120 229 4.05e-13 70.5
MS.gene28430.t1 AT3G18930 35.652 115 59 4 113 217 120 229 4.05e-13 70.5
MS.gene28430.t1 AT4G17920 41.026 78 44 1 110 185 78 155 4.73e-13 69.3
MS.gene28430.t1 AT5G43420 26.190 168 97 3 61 207 38 199 5.02e-13 70.1
MS.gene28430.t1 AT4G10150 34.940 83 52 1 104 186 77 157 5.53e-13 68.2
MS.gene28430.t1 AT3G11110 29.530 149 99 2 34 182 7 149 7.07e-13 66.2
MS.gene28430.t1 AT2G46160 36.145 83 46 1 113 188 104 186 7.30e-13 67.4
MS.gene28430.t1 AT2G46160 36.145 83 46 1 113 188 104 186 7.30e-13 67.4
MS.gene28430.t1 AT5G58580 39.474 76 46 0 111 186 109 184 7.63e-13 68.9
MS.gene28430.t1 AT5G36001 61.702 47 18 0 137 183 261 307 1.08e-12 68.6
MS.gene28430.t1 AT2G46495 40.789 76 43 1 109 182 259 334 1.15e-12 68.6
MS.gene28430.t1 AT2G46495 40.789 76 43 1 109 182 287 362 1.17e-12 68.9
MS.gene28430.t1 AT5G53110 38.889 72 43 1 113 183 302 373 1.41e-12 68.6
MS.gene28430.t1 AT2G47560 29.333 150 97 3 36 182 7 150 1.66e-12 66.6
MS.gene28430.t1 AT3G19140 39.437 71 41 1 113 183 54 122 1.68e-12 64.7
MS.gene28430.t1 AT5G40250 29.252 147 78 5 61 186 48 189 2.49e-12 67.8
MS.gene28430.t1 AT5G55900 46.154 78 27 1 222 284 62 139 5.60e-12 67.0
MS.gene28430.t1 AT3G61550 30.357 112 61 2 87 188 80 184 5.80e-12 64.7
MS.gene28430.t1 AT2G34000 36.000 75 45 1 112 183 60 134 1.23e-11 62.4
MS.gene28430.t1 AT4G26620 43.590 78 29 1 222 284 74 151 1.51e-11 65.9
MS.gene28430.t1 AT2G17730 38.667 75 44 1 111 183 165 239 1.53e-11 64.3
MS.gene28430.t1 AT2G18670 33.333 75 50 0 108 182 78 152 1.57e-11 63.2
MS.gene28430.t1 AT4G35840 38.667 75 44 1 111 183 160 234 1.80e-11 63.9
MS.gene28430.t1 AT4G26400 44.118 68 30 2 115 182 223 282 1.90e-11 65.1
MS.gene28430.t1 AT4G26400 44.118 68 30 2 115 182 223 282 1.90e-11 65.1
MS.gene28430.t1 AT2G42350 38.028 71 42 1 112 182 74 142 3.11e-11 62.8
MS.gene28430.t1 AT5G56340 44.118 68 30 2 115 182 241 300 5.78e-11 63.9
MS.gene28430.t1 AT1G53010 28.249 177 75 4 54 188 12 178 5.97e-11 61.2
MS.gene28430.t1 AT1G35630 43.478 69 37 2 116 183 209 276 6.37e-11 63.2
MS.gene28430.t1 AT3G20395 33.663 101 63 2 82 182 117 213 7.15e-11 62.0

Find 86 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCAACCAACTCATATTT+TGG 0.111404 4.4:-8783257 MS.gene28430:CDS
ATTGATGATCAATTGGATTT+TGG 0.218436 4.4:+8783315 None:intergenic
TTTCCCGGATTTGGGTATTC+AGG 0.256711 4.4:-8780720 MS.gene28430:CDS
TGGTGCCAGAAACTATTATT+AGG 0.292849 4.4:-8782785 MS.gene28430:CDS
AGACGTGTCGGCGACGAGTT+TGG 0.328105 4.4:+8780888 None:intergenic
TGAATCCTAATAATAGTTTC+TGG 0.344198 4.4:+8782780 None:intergenic
TCTATGCACACCCCTACTAT+TGG 0.373134 4.4:-8780444 MS.gene28430:CDS
GACGTGTCGGCGACGAGTTT+GGG 0.377781 4.4:+8780889 None:intergenic
TGGAAACCATGATTGCATTT+AGG 0.379959 4.4:+8782924 None:intergenic
GAGACTTGTCGAAAGATTGT+TGG 0.388982 4.4:-8782834 MS.gene28430:CDS
CAACAACAATTCACCTCAAT+TGG 0.391598 4.4:-8783087 MS.gene28430:CDS
TTGCATTAAACTTCATCATA+AGG 0.400685 4.4:-8783166 MS.gene28430:CDS
AAGGAGGCACTGTCTGCACT+TGG 0.408731 4.4:+8782857 None:intergenic
GCACACCCCTACTATTGGTT+GGG 0.409932 4.4:-8780439 MS.gene28430:CDS
AGTTACATTCAAAAGCTTCA+TGG 0.410074 4.4:+8782748 None:intergenic
GTAGGGGTGTGCATAGAAAC+TGG 0.418725 4.4:+8780450 None:intergenic
AGCTTCGACGCGTGGTGGCC+AGG 0.426267 4.4:+8780930 None:intergenic
ATGTTCGTTGCATCGACAAA+TGG 0.447158 4.4:-8782902 MS.gene28430:CDS
GGTTCTCCCCAACCAATAGT+AGG 0.449596 4.4:+8780432 None:intergenic
GTTGGTGATTGATGATCAAT+TGG 0.451468 4.4:+8783308 None:intergenic
GGGTATTCAGGGAAGGTTCA+CGG 0.454432 4.4:-8780708 MS.gene28430:CDS
CTTCCCTGAATACCCAAATC+CGG 0.458777 4.4:+8780716 None:intergenic
TCAGTTGAATAACTTACTGT+TGG 0.468597 4.4:+8783026 None:intergenic
AGCACCGCCTCCTATGTTGC+TGG 0.469981 4.4:-8782805 MS.gene28430:CDS
TGCACACCCCTACTATTGGT+TGG 0.479924 4.4:-8780440 MS.gene28430:CDS
CAGTTGAATAACTTACTGTT+GGG 0.481542 4.4:+8783027 None:intergenic
GGGTCGGTTACCCATTAAAA+CGG 0.484395 4.4:+8780571 None:intergenic
ATTCCTTTGTTGACCAATTG+AGG 0.485311 4.4:+8783074 None:intergenic
GTTCACGACGGCTGTGAGAA+AGG 0.496737 4.4:-8780653 MS.gene28430:CDS
TTCCCGGATTTGGGTATTCA+GGG 0.498316 4.4:-8780719 MS.gene28430:CDS
TGAGTTGAAACTACCAAGCT+TGG 0.499143 4.4:-8783006 MS.gene28430:CDS
TTGTTGAATCTCACACAAGA+AGG 0.499335 4.4:-8783346 MS.gene28430:CDS
GGGAGAACCGTTAGTGACAA+TGG 0.501780 4.4:-8780418 MS.gene28430:CDS
AGGAGGCACTGTCTGCACTT+GGG 0.504991 4.4:+8782858 None:intergenic
GAGTTCACGGTGGCTGAGAA+AGG 0.506307 4.4:-8780675 MS.gene28430:CDS
GGAGGCACTGTCTGCACTTG+GGG 0.507612 4.4:+8782859 None:intergenic
CGCGTGGTGGCCAGGAGAGG+TGG 0.507882 4.4:+8780938 None:intergenic
GAGAAAGGTTCACGCGAGAA+AGG 0.510119 4.4:-8780638 MS.gene28430:CDS
GTTTCTGGCACCAGCAACAT+AGG 0.512148 4.4:+8782795 None:intergenic
GGAGAACCGTTAGTGACAAT+GGG 0.520817 4.4:-8780417 MS.gene28430:CDS
CGGATTTGGGTATTCAGGGA+AGG 0.525323 4.4:-8780715 MS.gene28430:CDS
GTTCTCCCCAACCAATAGTA+GGG 0.528199 4.4:+8780433 None:intergenic
GTCGTAGGAGTCGACGGTGC+CGG 0.528314 4.4:+8780987 None:intergenic
GGAATTCACGAAAGGTGAAA+AGG 0.529132 4.4:-8782958 MS.gene28430:CDS
CGGTGGTTGAGAAGAGTTCA+CGG 0.532406 4.4:-8780688 MS.gene28430:CDS
TTGTCGAAAGATTGTTGGAT+CGG 0.534154 4.4:-8782829 MS.gene28430:CDS
GAGGAAAACGTGACGGTCGT+AGG 0.535068 4.4:+8780972 None:intergenic
TGTGAAGCGAGTGAGGAAGA+TGG 0.535644 4.4:-8780767 MS.gene28430:CDS
GGTCGGTTACCCATTAAAAC+GGG 0.540827 4.4:+8780572 None:intergenic
TTCCCTGAATACCCAAATCC+GGG 0.541228 4.4:+8780717 None:intergenic
ACGAATTCCAAAATATGAGT+TGG 0.554133 4.4:+8783250 None:intergenic
GTGAGAACAGGGGACGTTGT+TGG 0.562716 4.4:+8783290 None:intergenic
ATTTGTCGATGCAACGAACA+TGG 0.562919 4.4:+8782904 None:intergenic
CGACGCGTGGTGGCCAGGAG+AGG 0.563545 4.4:+8780935 None:intergenic
GTTTAACCCATTGTCACTAA+CGG 0.564585 4.4:+8780411 None:intergenic
GGCACCAGCAACATAGGAGG+CGG 0.572884 4.4:+8782801 None:intergenic
ATACACTCAGTATCCAAGCT+TGG 0.576598 4.4:+8782993 None:intergenic
AATATGAGTTGGTTGAATTG+TGG 0.580483 4.4:+8783261 None:intergenic
ATGGAGAAGAGGTAAAGTAA+AGG 0.584415 4.4:+8783376 None:intergenic
GAGATTCAACAAGAAGCTCA+TGG 0.584576 4.4:+8783357 None:intergenic
TCGGCGACGAGTTTGGGAAG+AGG 0.585120 4.4:+8780895 None:intergenic
ACAAGAAGCTCATGGAGAAG+AGG 0.587733 4.4:+8783365 None:intergenic
TCGTCGGAAGCTTCGACGCG+TGG 0.588689 4.4:+8780922 None:intergenic
CACACCCCTACTATTGGTTG+GGG 0.590248 4.4:-8780438 MS.gene28430:CDS
TGAGTGTATTATATGCCTCT+CGG 0.594238 4.4:-8782979 MS.gene28430:CDS
CAATCTTTCGACAAGTCTCA+AGG 0.597223 4.4:+8782838 None:intergenic
CACTGTGTGTGAAGCGAGTG+AGG 0.597959 4.4:-8780774 MS.gene28430:CDS
TCAAAAGCTTCATGGTCTAG+TGG 0.599238 4.4:+8782756 None:intergenic
TCGGAAGCTTCGACGCGTGG+TGG 0.614659 4.4:+8780925 None:intergenic
TCACCTCAATTGGTCAACAA+AGG 0.615305 4.4:-8783077 MS.gene28430:CDS
CGACGAGTTTGGGAAGAGGA+TGG 0.621819 4.4:+8780899 None:intergenic
GTGACGGTCGTAGGAGTCGA+CGG 0.624834 4.4:+8780981 None:intergenic
ACCGACACAGGTAAACTCGC+CGG 0.626604 4.4:-8781006 MS.gene28430:intron
GAGGAAGATGGATTCTCCGA+CGG 0.627284 4.4:-8780755 MS.gene28430:CDS
TGCCTCTCGGAATTCACGAA+AGG 0.628377 4.4:-8782966 MS.gene28430:CDS
CACCTTTCGTGAATTCCGAG+AGG 0.634223 4.4:+8782964 None:intergenic
TCTGGCACCAGCAACATAGG+AGG 0.642103 4.4:+8782798 None:intergenic
TTTGGGAAGAGGATGGTCGT+CGG 0.656732 4.4:+8780906 None:intergenic
TTCTCCCCAACCAATAGTAG+GGG 0.657929 4.4:+8780434 None:intergenic
GAAAATCAAAACATCACCGT+CGG 0.658320 4.4:+8780739 None:intergenic
TGTAGTAGAGGAAAACGTGA+CGG 0.664243 4.4:+8780965 None:intergenic
GGTTACCCATTAAAACGGGT+CGG 0.684993 4.4:+8780576 None:intergenic
TGGCTGAGAAAGGTTCACGA+CGG 0.749893 4.4:-8780665 MS.gene28430:CDS
TATTCAGGGAAGGTTCACGG+TGG 0.757645 4.4:-8780705 MS.gene28430:CDS
TCTTTCGACAAGTCTCAAGG+AGG 0.776052 4.4:+8782841 None:intergenic
TGGTTGAGAAGAGTTCACGG+TGG 0.855000 4.4:-8780685 MS.gene28430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTTTTTTTTTTTTAA+AGG + chr4.4:8781753-8781772 None:intergenic 0.0%
!! AAAATATAAATATAACAATG+AGG - chr4.4:8782219-8782238 MS.gene28430:intron 10.0%
!! TAAATAATTCAAAAAAAATG+AGG - chr4.4:8782306-8782325 MS.gene28430:intron 10.0%
!!! AAATTGAATAATTTACTTTT+TGG + chr4.4:8781408-8781427 None:intergenic 10.0%
!!! CATTATTTTATTTTTTTACA+AGG + chr4.4:8782111-8782130 None:intergenic 10.0%
!!! TAAATGGATTTTTTTAAAAT+CGG - chr4.4:8782423-8782442 MS.gene28430:intron 10.0%
!! TACTATTATTATCTATTCTA+AGG - chr4.4:8781637-8781656 MS.gene28430:intron 15.0%
!! TTATAAGATTATGAATTCAT+TGG + chr4.4:8781263-8781282 None:intergenic 15.0%
!!! AGTCTTTATTTTTTTTTACA+AGG + chr4.4:8781975-8781994 None:intergenic 15.0%
!!! ATAATTTATTTTTCAATCAG+CGG - chr4.4:8781778-8781797 MS.gene28430:intron 15.0%
!!! ATTACTACAAACTTTTTATA+GGG + chr4.4:8781841-8781860 None:intergenic 15.0%
!!! GTCTTTATTTTTTTTTACAA+GGG + chr4.4:8781974-8781993 None:intergenic 15.0%
!!! TTTTATTAGAAAAATATCGA+TGG + chr4.4:8782181-8782200 None:intergenic 15.0%
!! AAAAAAAATGAGGATACTAT+TGG - chr4.4:8782316-8782335 MS.gene28430:intron 20.0%
!! AAAAATATCTTTACTGCAAA+AGG + chr4.4:8782010-8782029 None:intergenic 20.0%
!! AAAATATCTTTACTGCAAAA+GGG + chr4.4:8782009-8782028 None:intergenic 20.0%
!! AAATGAAAGGGTAAAAAAAA+CGG + chr4.4:8782586-8782605 None:intergenic 20.0%
!! AACTACTTTCACAAAATAAA+TGG - chr4.4:8782407-8782426 MS.gene28430:intron 20.0%
!! AGCTACATTTAAAAATCTTA+AGG + chr4.4:8780669-8780688 None:intergenic 20.0%
!! ATAGTAATTTCTTTGATCAT+AGG + chr4.4:8781623-8781642 None:intergenic 20.0%
!! GAAATGAAAATGAAATGAAA+GGG + chr4.4:8782598-8782617 None:intergenic 20.0%
!! TAATATAAATGACTACAAGT+AGG - chr4.4:8781480-8781499 MS.gene28430:intron 20.0%
!! TGAAATGAAAATGAAATGAA+AGG + chr4.4:8782599-8782618 None:intergenic 20.0%
!!! ATTTTAAACCAGATTAAAAC+GGG + chr4.4:8783257-8783276 None:intergenic 20.0%
!!! CATTACTACAAACTTTTTAT+AGG + chr4.4:8781842-8781861 None:intergenic 20.0%
!!! CATTTTAAACCAGATTAAAA+CGG + chr4.4:8783258-8783277 None:intergenic 20.0%
!!! TTTTCTTAGAAAAATATCGA+TGG + chr4.4:8782042-8782061 None:intergenic 20.0%
! AAGTAAATATGTGTATGAAG+TGG - chr4.4:8781328-8781347 MS.gene28430:intron 25.0%
! AGTAAATATGTGTATGAAGT+GGG - chr4.4:8781329-8781348 MS.gene28430:intron 25.0%
! CTAGATTTATGGATACAATT+TGG - chr4.4:8782377-8782396 MS.gene28430:intron 25.0%
! GCATGTATTCCTAAAATTTA+TGG + chr4.4:8782251-8782270 None:intergenic 25.0%
! TAAAAAGAGCTAAATCAGTT+TGG - chr4.4:8782755-8782774 MS.gene28430:CDS 25.0%
! TCAAATAGAGAAAACTGAAA+AGG + chr4.4:8781587-8781606 None:intergenic 25.0%
! TCAGGTAAAAAATCTAATTC+AGG + chr4.4:8782523-8782542 None:intergenic 25.0%
! TGAATCCTAATAATAGTTTC+TGG + chr4.4:8781028-8781047 None:intergenic 25.0%
! TTGCATTAAACTTCATCATA+AGG - chr4.4:8780639-8780658 MS.gene28430:CDS 25.0%
! TTGTATCCATAAATCTAGTT+TGG + chr4.4:8782375-8782394 None:intergenic 25.0%
! TTTACAAGGGAAAAATATCT+TGG + chr4.4:8781961-8781980 None:intergenic 25.0%
!! AAAGATCGAATACAAGATTT+TGG - chr4.4:8781453-8781472 MS.gene28430:intron 25.0%
!! AAGCTTTTGAATGTAACTAT+AGG - chr4.4:8781062-8781081 MS.gene28430:intron 25.0%
!! AATTCAACCAACTCATATTT+TGG - chr4.4:8780548-8780567 MS.gene28430:intron 25.0%
!! AGCTTTTGAATGTAACTATA+GGG - chr4.4:8781063-8781082 MS.gene28430:intron 25.0%
!!! AATTTACTTTTTGGCTTTGT+CGG + chr4.4:8781399-8781418 None:intergenic 25.0%
!!! ATTATCTATTCTAAGGTGAA+TGG - chr4.4:8781644-8781663 MS.gene28430:intron 25.0%
!!! ATTGATGATCAATTGGATTT+TGG + chr4.4:8780493-8780512 None:intergenic 25.0%
!!! CAGTAAAGATATTTTTCAAG+TGG - chr4.4:8782014-8782033 MS.gene28430:intron 25.0%
!!! TTATCTATTCTAAGGTGAAT+GGG - chr4.4:8781645-8781664 MS.gene28430:intron 25.0%
AAAACATAGTCACATATCAC+AGG + chr4.4:8781549-8781568 None:intergenic 30.0%
ACGAATTCCAAAATATGAGT+TGG + chr4.4:8780558-8780577 None:intergenic 30.0%
ACTGAACCAAACTAGATTTA+TGG - chr4.4:8782366-8782385 MS.gene28430:intron 30.0%
AGAAAACTGAAAAGGAGAAT+GGG + chr4.4:8781579-8781598 None:intergenic 30.0%
AGGTAAAAAAAAAACGGTTC+AGG + chr4.4:8782560-8782579 None:intergenic 30.0%
AGTTACATTCAAAAGCTTCA+TGG + chr4.4:8781060-8781079 None:intergenic 30.0%
ATCTAATTCAGGACAAAAAC+TGG + chr4.4:8782512-8782531 None:intergenic 30.0%
CAGTTGAATAACTTACTGTT+GGG + chr4.4:8780781-8780800 None:intergenic 30.0%
CGGTTCAGGTAAAAAAAAAA+CGG + chr4.4:8782566-8782585 None:intergenic 30.0%
GACTACAAGTAGGAATATTA+TGG - chr4.4:8781490-8781509 MS.gene28430:intron 30.0%
GATCAACTTATTCTCAACAT+CGG + chr4.4:8781291-8781310 None:intergenic 30.0%
GTAAATATGTGTATGAAGTG+GGG - chr4.4:8781330-8781349 MS.gene28430:intron 30.0%
TAGAAAAGCCCAGATTAAAA+CGG + chr4.4:8783298-8783317 None:intergenic 30.0%
TAGGGAAGAAAGTAGATAAA+TGG - chr4.4:8781081-8781100 MS.gene28430:intron 30.0%
TCAGTTGAATAACTTACTGT+TGG + chr4.4:8780782-8780801 None:intergenic 30.0%
! AAATCTAGTTTGGTTCAGTT+TGG + chr4.4:8782365-8782384 None:intergenic 30.0%
! AAATGAGGATACTATTGGTT+AGG - chr4.4:8782321-8782340 MS.gene28430:intron 30.0%
! AATGAGGATACTATTGGTTA+GGG - chr4.4:8782322-8782341 MS.gene28430:intron 30.0%
! ACTACAAACTTTTTATAGGG+TGG + chr4.4:8781838-8781857 None:intergenic 30.0%
! AGGTGTGATCCATAAATTTT+AGG - chr4.4:8782239-8782258 MS.gene28430:intron 30.0%
! ATCGAATACAAGATTTTGGA+AGG - chr4.4:8781457-8781476 MS.gene28430:intron 30.0%
! ATGTGTTTTCAGATCTGAAA+TGG - chr4.4:8783202-8783221 MS.gene28430:CDS 30.0%
! ATTTTGCCTAAATGCAATCA+TGG - chr4.4:8780875-8780894 MS.gene28430:intron 30.0%
! CTACAAACTTTTTATAGGGT+GGG + chr4.4:8781837-8781856 None:intergenic 30.0%
! GTTCACCATATTTTCAGTTT+GGG - chr4.4:8783323-8783342 MS.gene28430:CDS 30.0%
! TAATCTGGGCTTTTCTAATT+TGG - chr4.4:8783301-8783320 MS.gene28430:CDS 30.0%
! TACAAACTTTTTATAGGGTG+GGG + chr4.4:8781836-8781855 None:intergenic 30.0%
! TAGCTCTTTTTACAGTAATG+AGG - chr4.4:8782678-8782697 MS.gene28430:intron 30.0%
!! AATATGAGTTGGTTGAATTG+TGG + chr4.4:8780547-8780566 None:intergenic 30.0%
!! TGTGTTTTCAGATCTGAAAT+GGG - chr4.4:8783203-8783222 MS.gene28430:CDS 30.0%
!!! ATTTACTTTTTGGCTTTGTC+GGG + chr4.4:8781398-8781417 None:intergenic 30.0%
AAGGGTAAAAAAAACGGTTC+AGG + chr4.4:8782580-8782599 None:intergenic 35.0%
AGAAAAGCCCAGATTAAAAC+GGG + chr4.4:8783297-8783316 None:intergenic 35.0%
ATGGAGAAGAGGTAAAGTAA+AGG + chr4.4:8780432-8780451 None:intergenic 35.0%
ATTCCTTTGTTGACCAATTG+AGG + chr4.4:8780734-8780753 None:intergenic 35.0%
CAACAACAATTCACCTCAAT+TGG - chr4.4:8780718-8780737 MS.gene28430:CDS 35.0%
CTTAAAGACAAACACCAAAC+TGG - chr4.4:8781357-8781376 MS.gene28430:intron 35.0%
GAAAATCAAAACATCACCGT+CGG + chr4.4:8783069-8783088 None:intergenic 35.0%
GAGAAAACTGAAAAGGAGAA+TGG + chr4.4:8781580-8781599 None:intergenic 35.0%
GCTTGTTCACTAAAATCTAG+AGG - chr4.4:8781205-8781224 MS.gene28430:intron 35.0%
GTACTTTACAGAAAAGAGTG+TGG + chr4.4:8781812-8781831 None:intergenic 35.0%
TAAACCAGATTAAAACGGGT+CGG + chr4.4:8783253-8783272 None:intergenic 35.0%
TCTAGAGGATAGAATTTGTC+TGG - chr4.4:8781220-8781239 MS.gene28430:intron 35.0%
TGAGTGTATTATATGCCTCT+CGG - chr4.4:8780826-8780845 MS.gene28430:intron 35.0%
TGGAAACCATGATTGCATTT+AGG + chr4.4:8780884-8780903 None:intergenic 35.0%
TTGTCGAAAGATTGTTGGAT+CGG - chr4.4:8780976-8780995 MS.gene28430:CDS 35.0%
TTGTTGAATCTCACACAAGA+AGG - chr4.4:8780459-8780478 MS.gene28430:intron 35.0%
! ACAAACTTTTTATAGGGTGG+GGG + chr4.4:8781835-8781854 None:intergenic 35.0%
! AGAGCGGTTGAAATTGTTTT+TGG + chr4.4:8782627-8782646 None:intergenic 35.0%
! ATGAGGATACTATTGGTTAG+GGG - chr4.4:8782323-8782342 MS.gene28430:intron 35.0%
! GGTTCACCATATTTTCAGTT+TGG - chr4.4:8783322-8783341 MS.gene28430:CDS 35.0%
! TGGTGCCAGAAACTATTATT+AGG - chr4.4:8781020-8781039 MS.gene28430:intron 35.0%
! TTCTTTAGGTTTCACCAGTT+TGG + chr4.4:8781374-8781393 None:intergenic 35.0%
!! CAGTTTGGTGTTTGTCTTTA+AGG + chr4.4:8781359-8781378 None:intergenic 35.0%
!! GTTGGTGATTGATGATCAAT+TGG + chr4.4:8780500-8780519 None:intergenic 35.0%
!!! GTTTTGATTTTCCCGGATTT+GGG - chr4.4:8783077-8783096 MS.gene28430:CDS 35.0%
!!! TCAGTGTTGTTTTCCCTTTT+TGG + chr4.4:8782715-8782734 None:intergenic 35.0%
!!! TGTTTTGATTTTCCCGGATT+TGG - chr4.4:8783076-8783095 MS.gene28430:CDS 35.0%
!!! TTTACTTTTTGGCTTTGTCG+GGG + chr4.4:8781397-8781416 None:intergenic 35.0%
ACGGTTCAGGTGAAAAAATC+AGG + chr4.4:8782547-8782566 None:intergenic 40.0%
AGGACAGTGATAAGCCAAAA+AGG - chr4.4:8782698-8782717 MS.gene28430:intron 40.0%
ATACACTCAGTATCCAAGCT+TGG + chr4.4:8780815-8780834 None:intergenic 40.0%
ATTTGTCGATGCAACGAACA+TGG + chr4.4:8780904-8780923 None:intergenic 40.0%
CAATCTTTCGACAAGTCTCA+AGG + chr4.4:8780970-8780989 None:intergenic 40.0%
CAGGTGAAAAAATCAGGTTC+AGG + chr4.4:8782541-8782560 None:intergenic 40.0%
GAATAGAAGAGAAGAGAGAG+CGG + chr4.4:8782643-8782662 None:intergenic 40.0%
GAGACTTGTCGAAAGATTGT+TGG - chr4.4:8780971-8780990 MS.gene28430:CDS 40.0%
GAGATTCAACAAGAAGCTCA+TGG + chr4.4:8780451-8780470 None:intergenic 40.0%
GCTGACCCAAACTGAAAATA+TGG + chr4.4:8783331-8783350 None:intergenic 40.0%
GGAATTCACGAAAGGTGAAA+AGG - chr4.4:8780847-8780866 MS.gene28430:intron 40.0%
GGACAGTGATAAGCCAAAAA+GGG - chr4.4:8782699-8782718 MS.gene28430:intron 40.0%
TAAAACGGGTCGGTTAAAAC+TGG + chr4.4:8783283-8783302 None:intergenic 40.0%
TCAAAAGCTTCATGGTCTAG+TGG + chr4.4:8781052-8781071 None:intergenic 40.0%
TCACCTCAATTGGTCAACAA+AGG - chr4.4:8780728-8780747 MS.gene28430:CDS 40.0%
TGTAGTAGAGGAAAACGTGA+CGG + chr4.4:8782843-8782862 None:intergenic 40.0%
! AACTGGTTCAGGACAAAAAC+CGG + chr4.4:8782495-8782514 None:intergenic 40.0%
! AGAGGTGGTTTTTGTAGTAG+AGG + chr4.4:8782855-8782874 None:intergenic 40.0%
! AGCTCTTTTTACCTGAGTGT+TGG + chr4.4:8782746-8782765 None:intergenic 40.0%
! ATGTTCGTTGCATCGACAAA+TGG - chr4.4:8780903-8780922 MS.gene28430:CDS 40.0%
! CAAACTTTTTATAGGGTGGG+GGG + chr4.4:8781834-8781853 None:intergenic 40.0%
! TGAGTTGAAACTACCAAGCT+TGG - chr4.4:8780799-8780818 MS.gene28430:intron 40.0%
! TTCAGGACAAAAACTGGTTC+AGG + chr4.4:8782506-8782525 None:intergenic 40.0%
!! TAACCGACCCGTTTTAATCT+GGG - chr4.4:8783247-8783266 MS.gene28430:CDS 40.0%
!! TTAACCGACCCGTTTTAATC+TGG - chr4.4:8783286-8783305 MS.gene28430:CDS 40.0%
!!! CGGTGATGTTTTGATTTTCC+CGG - chr4.4:8783070-8783089 MS.gene28430:CDS 40.0%
!!! TGTGGTTTTGTGTGAGAACA+GGG + chr4.4:8780529-8780548 None:intergenic 40.0%
!!! TTGTGGTTTTGTGTGAGAAC+AGG + chr4.4:8780530-8780549 None:intergenic 40.0%
AAACGAACCTTGAGACGTGT+CGG + chr4.4:8782932-8782951 None:intergenic 45.0%
AAGCCCAGATTAAAACGGGT+CGG + chr4.4:8783293-8783312 None:intergenic 45.0%
ACAAGAAGCTCATGGAGAAG+AGG + chr4.4:8780443-8780462 None:intergenic 45.0%
ACTACAAAAACCACCTCTCC+TGG - chr4.4:8782857-8782876 MS.gene28430:CDS 45.0%
AGTTCAGTCCAGTTCAGAAC+CGG - chr4.4:8782454-8782473 MS.gene28430:intron 45.0%
CTTCCCTGAATACCCAAATC+CGG + chr4.4:8783092-8783111 None:intergenic 45.0%
GGAGAACCGTTAGTGACAAT+GGG - chr4.4:8783388-8783407 MS.gene28430:CDS 45.0%
GGGTCGGTTACCCATTAAAA+CGG + chr4.4:8783237-8783256 None:intergenic 45.0%
GGTCGGTTACCCATTAAAAC+GGG + chr4.4:8783236-8783255 None:intergenic 45.0%
GGTTACCCATTAAAACGGGT+CGG + chr4.4:8783232-8783251 None:intergenic 45.0%
GTTCTCCCCAACCAATAGTA+GGG + chr4.4:8783375-8783394 None:intergenic 45.0%
TCTATGCACACCCCTACTAT+TGG - chr4.4:8783361-8783380 MS.gene28430:CDS 45.0%
TCTTTCGACAAGTCTCAAGG+AGG + chr4.4:8780967-8780986 None:intergenic 45.0%
TGAGAGTAACACCAACACTC+AGG - chr4.4:8782732-8782751 MS.gene28430:intron 45.0%
TTCCCGGATTTGGGTATTCA+GGG - chr4.4:8783086-8783105 MS.gene28430:CDS 45.0%
TTCCCTGAATACCCAAATCC+GGG + chr4.4:8783091-8783110 None:intergenic 45.0%
TTCTCCCCAACCAATAGTAG+GGG + chr4.4:8783374-8783393 None:intergenic 45.0%
TTTCCCGGATTTGGGTATTC+AGG - chr4.4:8783085-8783104 MS.gene28430:CDS 45.0%
! ATTGGTTAGGGGTGTGCAAA+TGG - chr4.4:8782334-8782353 MS.gene28430:intron 45.0%
! TTCAGGACAAAAACCGGTTC+AGG + chr4.4:8782489-8782508 None:intergenic 45.0%
!! GGTAACCGACCCGTTTTAAT+GGG - chr4.4:8783224-8783243 MS.gene28430:CDS 45.0%
!! GTAACCGACCCGTTTTAATC+TGG - chr4.4:8783246-8783265 MS.gene28430:CDS 45.0%
!!! GTGGTTTTGTGTGAGAACAG+GGG + chr4.4:8780528-8780547 None:intergenic 45.0%
!!! ATTATTTTATTTTTTTACAA+GGG + chr4.4:8782110-8782129 None:intergenic 5.0%
CACCTTTCGTGAATTCCGAG+AGG + chr4.4:8780844-8780863 None:intergenic 50.0%
CGGATTTGGGTATTCAGGGA+AGG - chr4.4:8783090-8783109 MS.gene28430:CDS 50.0%
CGGTGGTTGAGAAGAGTTCA+CGG - chr4.4:8783117-8783136 MS.gene28430:CDS 50.0%
GAGAAAGGTTCACGCGAGAA+AGG - chr4.4:8783167-8783186 MS.gene28430:CDS 50.0%
GGGAGAACCGTTAGTGACAA+TGG - chr4.4:8783387-8783406 MS.gene28430:CDS 50.0%
GGGTATTCAGGGAAGGTTCA+CGG - chr4.4:8783097-8783116 MS.gene28430:CDS 50.0%
GGTTCTCCCCAACCAATAGT+AGG + chr4.4:8783376-8783395 None:intergenic 50.0%
TATTCAGGGAAGGTTCACGG+TGG - chr4.4:8783100-8783119 MS.gene28430:CDS 50.0%
TGCACACCCCTACTATTGGT+TGG - chr4.4:8783365-8783384 MS.gene28430:CDS 50.0%
TGCCTCTCGGAATTCACGAA+AGG - chr4.4:8780839-8780858 MS.gene28430:intron 50.0%
TGGCTGAGAAAGGTTCACGA+CGG - chr4.4:8783140-8783159 MS.gene28430:CDS 50.0%
TGGTTGAGAAGAGTTCACGG+TGG - chr4.4:8783120-8783139 MS.gene28430:CDS 50.0%
TGTGAAGCGAGTGAGGAAGA+TGG - chr4.4:8783038-8783057 MS.gene28430:CDS 50.0%
TTTGGGAAGAGGATGGTCGT+CGG + chr4.4:8782902-8782921 None:intergenic 50.0%
! CACACCCCTACTATTGGTTG+GGG - chr4.4:8783367-8783386 MS.gene28430:CDS 50.0%
! GAGGAAGATGGATTCTCCGA+CGG - chr4.4:8783050-8783069 MS.gene28430:CDS 50.0%
! GCACACCCCTACTATTGGTT+GGG - chr4.4:8783366-8783385 MS.gene28430:CDS 50.0%
! GGGTAACCGACCCGTTTTAA+TGG - chr4.4:8783223-8783242 MS.gene28430:CDS 50.0%
! GTAGGGGTGTGCATAGAAAC+TGG + chr4.4:8783358-8783377 None:intergenic 50.0%
! TTTACCTGTGTCGGTGGAAG+AGG + chr4.4:8782794-8782813 None:intergenic 50.0%
!! AGCATGAACCGGTTCTGAAC+TGG + chr4.4:8782465-8782484 None:intergenic 50.0%
!! GTTTCTGGCACCAGCAACAT+AGG + chr4.4:8781013-8781032 None:intergenic 50.0%
!! TGGCTTTGTCGGGGTTCTTT+AGG + chr4.4:8781388-8781407 None:intergenic 50.0%
ACCGACACAGGTAAACTCGC+CGG - chr4.4:8782799-8782818 MS.gene28430:CDS 55.0%
CACTGTGTGTGAAGCGAGTG+AGG - chr4.4:8783031-8783050 MS.gene28430:CDS 55.0%
CCGGTTCAGGTAGCATGAAC+CGG + chr4.4:8782476-8782495 None:intergenic 55.0%
CCGGTTCATGCTACCTGAAC+CGG - chr4.4:8782473-8782492 MS.gene28430:intron 55.0%
CGACGAGTTTGGGAAGAGGA+TGG + chr4.4:8782909-8782928 None:intergenic 55.0%
GAGGAAAACGTGACGGTCGT+AGG + chr4.4:8782836-8782855 None:intergenic 55.0%
GAGTTCACGGTGGCTGAGAA+AGG - chr4.4:8783130-8783149 MS.gene28430:CDS 55.0%
GATACCTCTTCCACCGACAC+AGG - chr4.4:8782787-8782806 MS.gene28430:CDS 55.0%
GTGAGAACAGGGGACGTTGT+TGG + chr4.4:8780518-8780537 None:intergenic 55.0%
GTTCACGACGGCTGTGAGAA+AGG - chr4.4:8783152-8783171 MS.gene28430:CDS 55.0%
TCTGGCACCAGCAACATAGG+AGG + chr4.4:8781010-8781029 None:intergenic 55.0%
!! AAGGAGGCACTGTCTGCACT+TGG + chr4.4:8780951-8780970 None:intergenic 55.0%
!! AGGAGGCACTGTCTGCACTT+GGG + chr4.4:8780950-8780969 None:intergenic 55.0%
AGCACCGCCTCCTATGTTGC+TGG - chr4.4:8781000-8781019 MS.gene28430:intron 60.0%
GCCGGCGAGTTTACCTGTGT+CGG + chr4.4:8782803-8782822 None:intergenic 60.0%
GGCACCAGCAACATAGGAGG+CGG + chr4.4:8781007-8781026 None:intergenic 60.0%
GGCGAGTTTACCTGTGTCGG+TGG + chr4.4:8782800-8782819 None:intergenic 60.0%
GTGACGGTCGTAGGAGTCGA+CGG + chr4.4:8782827-8782846 None:intergenic 60.0%
TCGGCGACGAGTTTGGGAAG+AGG + chr4.4:8782913-8782932 None:intergenic 60.0%
! AGACGTGTCGGCGACGAGTT+TGG + chr4.4:8782920-8782939 None:intergenic 60.0%
! GACGTGTCGGCGACGAGTTT+GGG + chr4.4:8782919-8782938 None:intergenic 60.0%
!! GGAGGCACTGTCTGCACTTG+GGG + chr4.4:8780949-8780968 None:intergenic 60.0%
CTCGTCGCCGACACGTCTCA+AGG - chr4.4:8782922-8782941 MS.gene28430:CDS 65.0%
GTCGTAGGAGTCGACGGTGC+CGG + chr4.4:8782821-8782840 None:intergenic 65.0%
TCGGAAGCTTCGACGCGTGG+TGG + chr4.4:8782883-8782902 None:intergenic 65.0%
TCGTCGGAAGCTTCGACGCG+TGG + chr4.4:8782886-8782905 None:intergenic 65.0%
AGCTTCGACGCGTGGTGGCC+AGG + chr4.4:8782878-8782897 None:intergenic 70.0%
CGACGCGTGGTGGCCAGGAG+AGG + chr4.4:8782873-8782892 None:intergenic 75.0%
CGCGTGGTGGCCAGGAGAGG+TGG + chr4.4:8782870-8782889 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 8780413 8783414 8780413 ID=MS.gene28430
chr4.4 mRNA 8780413 8783414 8780413 ID=MS.gene28430.t1;Parent=MS.gene28430
chr4.4 exon 8782754 8783414 8782754 ID=MS.gene28430.t1.exon1;Parent=MS.gene28430.t1
chr4.4 CDS 8782754 8783414 8782754 ID=cds.MS.gene28430.t1;Parent=MS.gene28430.t1
chr4.4 exon 8780903 8781018 8780903 ID=MS.gene28430.t1.exon2;Parent=MS.gene28430.t1
chr4.4 CDS 8780903 8781018 8780903 ID=cds.MS.gene28430.t1;Parent=MS.gene28430.t1
chr4.4 exon 8780582 8780804 8780582 ID=MS.gene28430.t1.exon3;Parent=MS.gene28430.t1
chr4.4 CDS 8780582 8780804 8780582 ID=cds.MS.gene28430.t1;Parent=MS.gene28430.t1
chr4.4 exon 8780413 8780477 8780413 ID=MS.gene28430.t1.exon4;Parent=MS.gene28430.t1
chr4.4 CDS 8780413 8780477 8780413 ID=cds.MS.gene28430.t1;Parent=MS.gene28430.t1
Gene Sequence

>MS.gene28430

ATGTCTGCTTCTACTTCCTTTACTTTACCTCTTCTCCATGAGCTTCTTGTTGAATCTCACACAAGAAGGTTGCTCTTCCAAAATCCAATTGATCATCAATCACCAACAACGTCCCCTGTTCTCACACAAAACCACAATTCAACCAACTCATATTTTGGAATTCGTGTACTTGATGCAAATGTTGTGATGATACTTGCAGTTCTATTGTGTGCTCTTATTTGCTCTCTTGCATTAAACTTCATCATAAGGCGTGCCTTAAGATTTTTAAATGTAGCTCTCAACAACGACTCTTCTTCGAGTTCAAGCAACAACAATTCACCTCAATTGGTCAACAAAGGAATCAAGAAGAAAGCTCTCAAGACATTCCCAACAGTAAGTTATTCAACTGAGTTGAAACTACCAAGCTTGGATACTGAGTGTATTATATGCCTCTCGGAATTCACGAAAGGTGAAAAGGTGCGCATTTTGCCTAAATGCAATCATGGTTTCCATGTTCGTTGCATCGACAAATGGCTAAAAGCACATTCATCATGCCCCAAGTGCAGACAGTGCCTCCTTGAGACTTGTCGAAAGATTGTTGGATCGGAAGCACCGCCTCCTATGTTGCTGGTGCCAGAAACTATTATTAGGATTCAGCCACTAGACCATGAAGCTTTTGAATGTAACTATAGGGAAGAAAGTAGATAAATGGTCATTTATAGTTTCAAACTTTTAGTTGCTTTAGTTTGACTTCATATATAAATTTATATATACATTAATTAAAATTTTGAGTGCAATTTCAAATTAGTTTTCGCTTGTTCACTAAAATCTAGAGGATAGAATTTGTCTGGAAGATCACATATATCATCCAATGAATTCATAATCTTATAAGCATTCCGATGTTGAGAATAAGTTGATCTTAAACTATATATGAAAAAGTAAATATGTGTATGAAGTGGGGTAACCTTAAAGACAAACACCAAACTGGTGAAACCTAAAGAACCCCGACAAAGCCAAAAAGTAAATTATTCAATTTTGAATAGAAAAAAAAAATCAAGAACAAAGATCGAATACAAGATTTTGGAAGGTAATATAAATGACTACAAGTAGGAATATTATGGTTGTTTTCTGATCTGTTAACTTCAAATTAAATACCTGTGATATGTGACTATGTTTTGAACTAACCCATTCTCCTTTTCAGTTTTCTCTATTTGATGAAAATATGACACCTATGATCAAAGAAATTACTATTATTATCTATTCTAAGGTGAATGGGTGCACTTTAAAGAATGAAATACTTGAAAATTGAAATATATGTACATGTAAGAAAAATAATGAAGTCAATTAAATCTCAATATCCTTTAAAAAAAAAAAAATTAAATCTCAATAATTTATTTTTCAATCAGCGGTTATTTTTCCACACTCTTTTCTGTAAAGTACCCCCCACCCTATAAAAAGTTTGTAGTAATGCAAGTGTAAAGCAGCTTTCTTAATACTAAATTTTACTTTATTTAGTTGAAGTTTTTAGATATTCTACTTCAGACTATAAAGATATTCTAATTCAGACCAAGATATTTTTCCCTTGTAAAAAAAAATAAAGACTAAGATATTTTTCCCTTTTGCAGTAAAGATATTTTTCAAGTGGATCCATCGATATTTTTCTAAGAAAATCTCACAATCAACGCTTCAAAATATAAATTTATAAAGATATTTTTCCCTTGTAAAAAAATAAAATAATGACTAAGATATTTTTCCCTTTTGCAGTAAAGATATTTTTCAAGTGGATCCATCGATATTTTTCTAATAAAATCTCACAATCAACGCTTCAAAATATAAATATAACAATGAGGTGTGATCCATAAATTTTAGGAATACATGCATAGTTAATTTCAAAAATATTTCTTAATTAAAATTTAAATAATTCAAAAAAAATGAGGATACTATTGGTTAGGGGTGTGCAAATGGTTTATCCAAACTGAACCAAACTAGATTTATGGATACAATTTGGATCAAAAAACTACTTTCACAAAATAAATGGATTTTTTTAAAATCGGTTCGATACAGTTCAGTCCAGTTCAGAACCGGTTCATGCTACCTGAACCGGTTTTTGTCCTGAACCAGTTTTTGTCCTGAATTAGATTTTTTACCTGAACCTGATTTTTTCACCTGAACCGTTTTTTTTTTACCTGAACCGTTTTTTTTACCCTTTCATTTCATTTTCATTTCAACTTACCAAAAACAATTTCAACCGCTCTCTCTTCTCTTCTATTCATTTCAATGATGTTTTAGCTCTTTTTACAGTAATGAGGACAGTGATAAGCCAAAAAGGGAAAACAACACTGAGAGTAACACCAACACTCAGGTAAAAAGAGCTAAATCAGTTTGGTATAATTCTGATACCTCTTCCACCGACACAGGTAAACTCGCCGGCACCGTCGACTCCTACGACCGTCACGTTTTCCTCTACTACAAAAACCACCTCTCCTGGCCACCACGCGTCGAAGCTTCCGACGACCATCCTCTTCCCAAACTCGTCGCCGACACGTCTCAAGGTTCGTTTCTTTCTCTCTCTCTTTCTGTCAATTTCGATACTGACATTAAAACTGATTTTGTTTGATTTGAATTTTGTAGACGAAAATCACTGTGTGTGAAGCGAGTGAGGAAGATGGATTCTCCGACGGTGATGTTTTGATTTTCCCGGATTTGGGTATTCAGGGAAGGTTCACGGTGGTTGAGAAGAGTTCACGGTGGCTGAGAAAGGTTCACGACGGCTGTGAGAAAGGTTCACGCGAGAAAGGTTTAGATCTGAAATGTGTTTTCAGATCTGAAATGGGTAACCGACCCGTTTTAATGGGTAACCGACCCGTTTTAATCTGGTTTAAAATGATCCAGTTTTAACCGACCCGTTTTAATCTGGGCTTTTCTAATTTGGTTCACCATATTTTCAGTTTGGGTCAGCTGATCCAGTTTCTATGCACACCCCTACTATTGGTTGGGGAGAACCGTTAGTGACAATGGGTTAA
Protein sequence

>MS.gene28430.t1

MSASTSFTLPLLHELLVESHTRRLLFQNPIDHQSPTTSPVLTQNHNSTNSYFGIRVLDANVVMILAVLLCALICSLALNFIIRRALRFLNVALNNDSSSSSSNNNSPQLVNKGIKKKALKTFPTVSYSTELKLPSLDTECIICLSEFTKGEKVRILPKCNHGFHVRCIDKWLKAHSSCPKCRQCLLETCRKIVGSEAPPPMLLVPETIIRIQPLDHEAFECKLAGTVDSYDRHVFLYYKNHLSWPPRVEASDDHPLPKLTKITVCEASEEDGFSDGDVLIFPDLGIQGRFTVVEKSSRWLRKVHDGCEKGSREKGLDLKCVFRSEMGNRPVLMADPVSMHTPTIGWGEPLVTMG