AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70250.t1 MTR_8g092990 90.909 231 21 0 1 231 1 231 1.07e-134 378
MS.gene70250.t1 MTR_8g093010 79.476 229 35 5 1 227 1 219 7.81e-123 347
MS.gene70250.t1 MTR_8g092840 80.973 226 42 1 1 226 1 225 6.68e-122 345
MS.gene70250.t1 MTR_8g092970 81.938 227 31 2 1 227 1 217 9.78e-122 345
MS.gene70250.t1 MTR_8g092930 80.176 227 43 2 1 227 1 225 2.36e-119 339
MS.gene70250.t1 MTR_8g092985 81.690 213 36 1 1 213 1 210 1.60e-117 334
MS.gene70250.t1 MTR_8g092960 76.991 226 41 2 1 226 1 215 2.60e-115 328
MS.gene70250.t1 MTR_3g095560 62.025 237 76 4 1 230 1 230 2.37e-94 276
MS.gene70250.t1 MTR_3g094000 50.000 146 67 2 54 198 36 176 4.60e-41 138
MS.gene70250.t1 MTR_7g073200 48.630 146 59 4 47 187 19 153 4.66e-40 136
MS.gene70250.t1 MTR_5g024730 43.827 162 74 6 55 208 26 178 4.93e-34 121
MS.gene70250.t1 MTR_3g116000 45.775 142 64 4 59 195 26 159 3.42e-33 119
MS.gene70250.t1 MTR_8g073120 44.755 143 67 5 55 194 19 152 2.13e-30 112
MS.gene70250.t1 MTR_4g094725 42.400 125 66 2 61 184 30 149 4.07e-27 102
MS.gene70250.t1 MTR_2g035800 38.060 134 67 3 56 184 23 145 2.61e-26 100
MS.gene70250.t1 MTR_3g071300 34.146 164 88 4 57 213 22 172 3.93e-22 89.7
MS.gene70250.t1 MTR_1g043410 28.659 164 104 4 62 216 35 194 6.83e-20 86.7
MS.gene70250.t1 MTR_7g113800 33.775 151 78 5 46 187 29 166 1.22e-19 86.7
MS.gene70250.t1 MTR_8g023400 45.570 79 40 2 114 191 92 168 5.27e-19 84.3
MS.gene70250.t1 MTR_5g082940 48.000 75 38 1 113 186 74 148 2.15e-18 79.7
MS.gene70250.t1 MTR_6g087150 33.577 137 90 1 48 184 27 162 2.38e-18 83.2
MS.gene70250.t1 MTR_7g117540 31.111 135 91 1 55 189 17 149 2.48e-18 82.0
MS.gene70250.t1 MTR_1g082550 29.530 149 94 4 43 187 32 173 3.36e-18 82.4
MS.gene70250.t1 MTR_4g120270 43.836 73 38 1 111 183 77 146 3.42e-18 81.3
MS.gene70250.t1 MTR_1g100737 37.879 132 70 3 63 190 49 172 1.26e-17 78.2
MS.gene70250.t1 MTR_2g436100 31.210 157 86 5 42 183 12 161 2.04e-17 79.3
MS.gene70250.t1 MTR_3g078450 35.652 115 71 2 89 202 51 163 2.16e-17 78.2
MS.gene70250.t1 MTR_1g492830 39.474 76 46 0 113 188 60 135 3.77e-17 77.8
MS.gene70250.t1 MTR_3g110190 32.576 132 79 4 62 189 15 140 4.14e-17 79.3
MS.gene70250.t1 MTR_2g096350 60.417 48 19 0 136 183 130 177 4.66e-17 77.4
MS.gene70250.t1 MTR_7g116220 40.000 80 46 1 111 188 67 146 5.09e-17 76.3
MS.gene70250.t1 MTR_5g064980 31.746 126 81 2 63 186 60 182 8.38e-17 78.6
MS.gene70250.t1 MTR_6g055030 36.975 119 66 3 67 183 57 168 8.96e-17 78.2
MS.gene70250.t1 MTR_3g464780 43.590 78 41 1 113 187 101 178 1.09e-16 77.0
MS.gene70250.t1 MTR_5g064990 30.000 150 100 4 53 199 55 202 1.35e-16 78.2
MS.gene70250.t1 MTR_7g076250 29.012 162 97 5 66 223 17 164 1.47e-16 75.5
MS.gene70250.t1 MTR_3g114500 33.871 124 67 5 64 183 1 113 1.78e-16 73.6
MS.gene70250.t1 MTR_4g056590 40.230 87 45 3 113 199 99 178 1.87e-16 75.1
MS.gene70250.t1 MTR_8g066780 26.425 193 120 3 59 229 48 240 3.74e-16 76.6
MS.gene70250.t1 MTR_3g099650 41.558 77 43 1 113 187 106 182 3.77e-16 76.6
MS.gene70250.t1 MTR_2g055790 35.772 123 68 3 66 187 34 146 4.01e-16 74.3
MS.gene70250.t1 MTR_5g071930 39.604 101 52 3 106 206 61 152 5.99e-16 73.9
MS.gene70250.t1 MTR_5g030010 36.275 102 62 2 107 208 115 213 8.16e-16 75.9
MS.gene70250.t1 MTR_8g089880 37.500 80 49 1 114 193 127 205 1.12e-15 74.3
MS.gene70250.t1 MTR_4g030070 36.364 88 54 1 104 191 75 160 1.26e-15 73.6
MS.gene70250.t1 MTR_4g129420 43.243 74 42 0 114 187 117 190 1.42e-15 75.5
MS.gene70250.t1 MTR_5g064890 31.897 116 76 2 74 187 86 200 1.52e-15 75.1
MS.gene70250.t1 MTR_7g116180 40.000 75 43 1 114 186 67 141 1.88e-15 72.0
MS.gene70250.t1 MTR_4g063070 43.662 71 39 1 114 183 308 378 2.11e-15 74.7
MS.gene70250.t1 MTR_1g070425 40.000 75 43 1 114 186 68 142 2.51e-15 71.6
MS.gene70250.t1 MTR_3g078470 37.113 97 59 2 91 186 54 149 2.65e-15 72.4
MS.gene70250.t1 MTR_5g076990 35.433 127 76 3 62 183 23 148 3.13e-15 73.6
MS.gene70250.t1 MTR_4g108830 34.694 98 62 1 87 184 122 217 4.77e-15 72.0
MS.gene70250.t1 MTR_7g108855 36.957 92 49 2 115 197 67 158 5.33e-15 71.2
MS.gene70250.t1 MTR_3g012550 28.030 132 92 3 53 183 21 150 5.61e-15 70.9
MS.gene70250.t1 MTR_4g093830 32.407 108 62 2 114 221 126 222 6.47e-15 73.2
MS.gene70250.t1 MTR_8g028465 32.710 107 55 3 111 217 87 176 7.55e-15 70.9
MS.gene70250.t1 MTR_2g009310 60.000 45 18 0 139 183 159 203 8.45e-15 73.2
MS.gene70250.t1 MTR_8g099495 38.961 77 46 1 108 183 313 389 9.96e-15 72.8
MS.gene70250.t1 MTR_5g092790 40.789 76 44 1 109 183 152 227 1.03e-14 71.2
MS.gene70250.t1 MTR_5g066250 41.429 70 40 1 114 183 134 202 1.41e-14 72.4
MS.gene70250.t1 MTR_4g108830 39.535 86 33 1 99 184 123 189 1.43e-14 70.1
MS.gene70250.t1 MTR_5g026720 28.467 137 84 4 54 183 6 135 1.47e-14 69.3
MS.gene70250.t1 MTR_5g009190 38.750 80 48 1 105 183 257 336 1.64e-14 72.0
MS.gene70250.t1 MTR_6g007737 31.387 137 79 4 62 186 71 204 1.75e-14 72.0
MS.gene70250.t1 MTR_1g074190 32.231 121 72 3 81 191 61 181 1.93e-14 71.6
MS.gene70250.t1 MTR_3g100400 41.429 70 40 1 114 183 130 198 1.94e-14 72.0
MS.gene70250.t1 MTR_6g007680 43.662 71 39 1 113 183 39 108 1.98e-14 68.9
MS.gene70250.t1 MTR_5g009210 38.750 80 48 1 105 183 278 357 2.54e-14 71.6
MS.gene70250.t1 MTR_5g026730 44.615 65 32 2 123 183 72 136 2.63e-14 68.9
MS.gene70250.t1 MTR_7g079280 30.579 121 69 3 106 225 72 178 2.87e-14 68.9
MS.gene70250.t1 MTR_2g090905 44.286 70 27 1 140 209 70 127 2.94e-14 67.4
MS.gene70250.t1 MTR_8g074900 42.466 73 37 1 114 186 158 225 4.71e-14 69.3
MS.gene70250.t1 MTR_3g091620 34.579 107 57 3 114 208 101 206 5.16e-14 70.5
MS.gene70250.t1 MTR_5g034790 41.096 73 42 1 114 186 116 187 7.97e-14 70.1
MS.gene70250.t1 MTR_1g026070 29.167 120 78 3 64 183 1 113 8.22e-14 66.6
MS.gene70250.t1 MTR_2g041200 36.082 97 53 1 96 183 55 151 8.83e-14 67.4
MS.gene70250.t1 MTR_5g022920 40.000 75 44 1 113 186 160 234 9.47e-14 68.6
MS.gene70250.t1 MTR_3g086700 36.458 96 55 2 94 183 22 117 9.59e-14 67.8
MS.gene70250.t1 MTR_7g079290 32.558 86 53 2 112 193 123 207 1.07e-13 68.2
MS.gene70250.t1 MTR_1g056350 35.165 91 55 2 114 201 74 163 1.23e-13 67.0
MS.gene70250.t1 MTR_5g009200 38.961 77 46 1 108 183 316 392 1.23e-13 69.7
MS.gene70250.t1 MTR_1g070140 36.842 76 48 0 108 183 97 172 1.30e-13 69.3
MS.gene70250.t1 MTR_5g092750 42.466 73 41 1 112 183 155 227 1.72e-13 67.8
MS.gene70250.t1 MTR_8g032690 43.077 65 34 1 141 205 19 80 1.77e-13 65.1
MS.gene70250.t1 MTR_8g072020 34.395 157 80 6 29 183 313 448 1.82e-13 69.3
MS.gene70250.t1 MTR_3g091200 39.437 71 42 1 114 183 303 373 1.90e-13 68.9
MS.gene70250.t1 MTR_3g087900 48.529 68 30 3 121 187 52 115 2.05e-13 66.2
MS.gene70250.t1 MTR_2g090950 59.091 44 18 0 140 183 122 165 2.16e-13 67.8
MS.gene70250.t1 MTR_3g086730 38.750 80 47 1 109 186 41 120 2.29e-13 67.4
MS.gene70250.t1 MTR_8g063660 38.667 75 45 1 114 188 68 141 2.40e-13 68.6
MS.gene70250.t1 MTR_7g059360 47.368 57 30 0 128 184 145 201 2.41e-13 67.4
MS.gene70250.t1 MTR_6g007670 43.478 69 37 1 115 183 9 75 2.53e-13 67.8
MS.gene70250.t1 MTR_6g007670 45.070 71 39 0 113 183 144 214 3.21e-13 67.4
MS.gene70250.t1 MTR_8g072020 34.395 157 80 6 29 183 352 487 2.58e-13 68.9
MS.gene70250.t1 MTR_4g106680 38.571 70 42 1 114 183 106 174 9.54e-13 66.6
MS.gene70250.t1 MTR_8g099515 42.254 71 40 1 114 183 309 379 1.04e-12 67.0
MS.gene70250.t1 MTR_8g019710 60.000 45 18 0 139 183 52 96 1.38e-12 63.5
MS.gene70250.t1 MTR_7g096690 26.471 136 79 5 62 183 14 142 1.90e-12 65.9
MS.gene70250.t1 MTR_4g116460 37.662 77 40 3 108 183 414 483 3.46e-12 65.5
MS.gene70250.t1 MTR_4g116460 37.662 77 40 3 108 183 457 526 3.72e-12 65.5
MS.gene70250.t1 MTR_8g071815 53.488 43 20 0 141 183 90 132 3.87e-12 63.2
MS.gene70250.t1 MTR_3g078120 31.200 125 81 4 62 183 5 127 4.50e-12 62.4
MS.gene70250.t1 MTR_7g116200 32.090 134 69 5 65 183 9 135 5.20e-12 62.8
MS.gene70250.t1 MTR_8g027745 35.897 78 49 1 107 183 279 356 6.10e-12 64.7
MS.gene70250.t1 MTR_4g022410 29.688 128 63 2 62 183 49 155 6.11e-12 62.8
MS.gene70250.t1 MTR_8g066740 31.496 127 81 3 62 183 23 148 9.25e-12 63.9
MS.gene70250.t1 MTR_2g079320 33.333 96 52 1 91 186 121 204 2.32e-11 61.2
MS.gene70250.t1 MTR_3g081030 34.343 99 55 2 120 218 100 188 2.67e-11 61.6
MS.gene70250.t1 MTR_4g106960 54.000 50 22 1 134 183 171 219 3.85e-11 62.0
MS.gene70250.t1 MTR_4g106960 54.000 50 22 1 134 183 171 219 4.26e-11 62.0
MS.gene70250.t1 MTR_4g106960 54.000 50 22 1 134 183 228 276 4.38e-11 62.0
MS.gene70250.t1 MTR_6g055030 53.659 41 19 0 143 183 97 137 4.67e-11 61.6
MS.gene70250.t1 MTR_4g106960 54.000 50 22 1 134 183 228 276 5.32e-11 62.0
MS.gene70250.t1 MTR_8g027980 33.333 75 48 1 117 189 93 167 5.50e-11 61.2
MS.gene70250.t1 MTR_3g078540 41.270 63 36 1 128 189 66 128 6.09e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70250.t1 AT1G49230 49.099 222 87 4 11 225 17 219 7.95e-65 201
MS.gene70250.t1 AT1G49220 47.059 204 87 6 21 216 23 213 1.75e-56 180
MS.gene70250.t1 AT1G49210 46.009 213 96 5 21 226 23 223 4.06e-56 179
MS.gene70250.t1 AT1G49200 44.860 214 97 6 21 226 24 224 4.00e-55 176
MS.gene70250.t1 AT3G18773 49.708 171 80 3 60 226 50 218 3.67e-51 166
MS.gene70250.t1 AT5G05280 51.429 140 61 2 58 197 37 169 2.93e-42 141
MS.gene70250.t1 AT3G10910 52.632 133 59 3 58 187 29 160 2.51e-40 137
MS.gene70250.t1 AT1G76410 43.448 145 75 3 47 189 13 152 4.82e-35 123
MS.gene70250.t1 AT1G20823 45.113 133 69 2 58 187 26 157 5.31e-34 121
MS.gene70250.t1 AT5G01880 39.572 187 84 6 12 197 1 159 1.58e-28 105
MS.gene70250.t1 AT2G17450 40.278 144 66 5 59 194 25 156 2.44e-27 103
MS.gene70250.t1 AT4G17245 40.299 134 63 3 52 183 27 145 3.28e-26 100
MS.gene70250.t1 AT4G35480 37.748 151 73 4 59 194 23 167 5.65e-26 100
MS.gene70250.t1 AT5G47610 37.879 132 70 3 52 183 30 149 1.02e-25 99.0
MS.gene70250.t1 AT4G40070 34.395 157 93 4 65 216 49 200 4.70e-20 87.4
MS.gene70250.t1 AT1G72200 31.902 163 86 5 57 213 58 201 9.27e-19 84.3
MS.gene70250.t1 AT1G22500 34.307 137 84 3 54 187 31 164 1.04e-18 84.0
MS.gene70250.t1 AT2G46494 29.255 188 121 5 2 183 177 358 5.28e-18 82.0
MS.gene70250.t1 AT2G35000 33.083 133 85 2 59 187 49 181 6.48e-18 82.0
MS.gene70250.t1 AT1G74410 39.560 91 54 1 96 186 132 221 1.16e-17 79.0
MS.gene70250.t1 AT1G72310 64.583 48 17 0 140 187 126 173 1.51e-17 80.5
MS.gene70250.t1 AT3G14320 47.887 71 35 1 114 184 63 131 1.80e-17 78.2
MS.gene70250.t1 AT5G57750 32.283 127 81 2 62 184 40 165 3.38e-17 77.4
MS.gene70250.t1 AT1G53820 50.000 62 30 1 140 200 119 180 5.66e-17 78.6
MS.gene70250.t1 AT3G05200 42.222 90 48 2 113 200 98 185 1.10e-16 78.6
MS.gene70250.t1 AT3G16720 41.975 81 44 2 113 193 93 170 1.21e-16 77.4
MS.gene70250.t1 AT1G28040 36.538 104 63 2 83 183 192 295 2.84e-16 76.6
MS.gene70250.t1 AT4G09100 32.787 122 75 3 64 183 11 127 3.11e-16 73.2
MS.gene70250.t1 AT4G17905 66.667 45 15 0 139 183 153 197 3.29e-16 76.6
MS.gene70250.t1 AT4G17905 66.667 45 15 0 139 183 179 223 3.32e-16 76.6
MS.gene70250.t1 AT2G20030 45.714 70 37 1 114 183 98 166 3.46e-16 77.0
MS.gene70250.t1 AT4G09120 45.205 73 38 1 113 183 93 165 4.66e-16 76.3
MS.gene70250.t1 AT3G03550 64.444 45 16 0 139 183 157 201 5.25e-16 76.3
MS.gene70250.t1 AT4G15975 38.636 88 52 1 96 183 33 118 7.91e-16 74.3
MS.gene70250.t1 AT3G62690 36.538 104 54 3 115 216 88 181 8.73e-16 74.7
MS.gene70250.t1 AT2G27940 36.782 87 53 1 108 194 109 193 9.78e-16 73.9
MS.gene70250.t1 AT5G17600 43.662 71 39 1 113 183 115 184 1.03e-15 75.5
MS.gene70250.t1 AT5G27420 36.893 103 48 3 111 210 92 180 1.06e-15 75.5
MS.gene70250.t1 AT4G09110 43.836 73 39 1 113 183 93 165 1.13e-15 74.7
MS.gene70250.t1 AT2G18650 43.243 74 42 0 114 187 99 172 1.55e-15 75.5
MS.gene70250.t1 AT2G34990 28.105 153 102 3 59 209 34 180 1.70e-15 74.7
MS.gene70250.t1 AT2G34990 28.105 153 102 3 59 209 12 158 1.78e-15 74.3
MS.gene70250.t1 AT1G23980 31.788 151 82 5 54 187 44 190 1.86e-15 74.7
MS.gene70250.t1 AT5G42200 42.254 71 40 1 113 183 77 146 1.97e-15 71.6
MS.gene70250.t1 AT1G04360 30.075 133 81 3 62 183 45 176 2.15e-15 74.7
MS.gene70250.t1 AT1G35330 42.105 76 42 1 113 186 99 174 3.06e-15 73.9
MS.gene70250.t1 AT2G42360 32.000 125 72 2 62 183 36 150 3.06e-15 72.8
MS.gene70250.t1 AT4G30400 41.429 70 41 0 114 183 107 176 3.19e-15 74.3
MS.gene70250.t1 AT4G28890 42.254 71 40 1 114 184 97 166 3.27e-15 74.3
MS.gene70250.t1 AT1G32361 34.043 141 82 5 84 223 101 231 3.79e-15 73.6
MS.gene70250.t1 AT4G10160 32.540 126 75 3 65 187 25 143 4.72e-15 72.0
MS.gene70250.t1 AT5G66070 43.243 74 39 2 113 186 151 221 5.60e-15 71.6
MS.gene70250.t1 AT5G66070 43.243 74 39 2 113 186 151 221 5.60e-15 71.6
MS.gene70250.t1 AT3G61550 28.966 145 76 4 88 222 80 207 6.44e-15 71.2
MS.gene70250.t1 AT5G66070 43.243 74 39 2 113 186 175 245 7.86e-15 71.6
MS.gene70250.t1 AT4G09130 42.667 75 41 1 111 183 88 162 8.93e-15 72.8
MS.gene70250.t1 AT2G46493 30.597 134 90 2 53 183 47 180 9.07e-15 70.5
MS.gene70250.t1 AT5G06490 32.692 104 54 2 112 209 94 187 9.67e-15 70.5
MS.gene70250.t1 AT2G46160 32.075 106 59 2 111 209 101 200 9.89e-15 70.9
MS.gene70250.t1 AT2G46160 32.075 106 59 2 111 209 101 200 9.89e-15 70.9
MS.gene70250.t1 AT1G72220 46.970 66 31 1 139 200 175 240 1.06e-14 72.8
MS.gene70250.t1 AT2G17730 41.892 74 41 1 113 184 166 239 1.26e-14 71.2
MS.gene70250.t1 AT5G05810 41.429 70 40 1 114 183 120 188 3.21e-14 71.2
MS.gene70250.t1 AT4G35840 41.892 74 41 1 113 184 161 234 3.48e-14 69.7
MS.gene70250.t1 AT2G25410 42.529 87 47 2 100 183 283 369 3.71e-14 71.2
MS.gene70250.t1 AT3G48030 38.356 73 45 0 114 186 102 174 3.74e-14 70.1
MS.gene70250.t1 AT4G33565 52.941 51 24 0 139 189 215 265 4.25e-14 70.9
MS.gene70250.t1 AT5G10380 37.975 79 48 1 109 187 104 181 4.31e-14 70.5
MS.gene70250.t1 AT3G48030 38.356 73 45 0 114 186 180 252 5.70e-14 70.5
MS.gene70250.t1 AT5G53110 37.333 75 46 1 110 183 298 372 9.12e-14 70.1
MS.gene70250.t1 AT2G35420 29.032 155 101 6 64 218 30 175 1.00e-13 68.9
MS.gene70250.t1 AT5G43420 27.273 143 83 2 62 183 38 180 1.32e-13 69.7
MS.gene70250.t1 AT2G18670 37.143 70 44 0 114 183 83 152 1.60e-13 67.0
MS.gene70250.t1 AT2G47560 43.056 72 40 1 113 183 79 150 1.65e-13 67.8
MS.gene70250.t1 AT5G36001 63.043 46 17 0 138 183 261 306 1.65e-13 68.9
MS.gene70250.t1 AT3G18930 42.105 76 37 2 140 214 155 224 1.85e-13 69.3
MS.gene70250.t1 AT3G18930 42.105 76 37 2 140 214 155 224 1.85e-13 69.3
MS.gene70250.t1 AT3G18930 42.105 76 37 2 140 214 155 224 1.85e-13 69.3
MS.gene70250.t1 AT2G35910 35.227 88 56 1 114 201 121 207 1.91e-13 67.4
MS.gene70250.t1 AT5G58580 41.333 75 44 0 113 187 110 184 2.37e-13 68.6
MS.gene70250.t1 AT4G17920 30.303 132 85 2 62 186 24 155 3.91e-13 67.4
MS.gene70250.t1 AT2G34000 37.333 75 44 1 113 184 60 134 3.93e-13 65.1
MS.gene70250.t1 AT3G19140 42.105 76 40 2 111 184 49 122 5.15e-13 64.7
MS.gene70250.t1 AT2G46495 40.789 76 43 1 110 183 287 362 6.68e-13 67.4
MS.gene70250.t1 AT5G40250 50.000 48 24 0 140 187 142 189 7.02e-13 67.4
MS.gene70250.t1 AT2G46495 40.789 76 43 1 110 183 259 334 7.14e-13 67.4
MS.gene70250.t1 AT5G07040 27.068 133 84 3 53 183 15 136 8.99e-13 64.3
MS.gene70250.t1 AT4G10150 30.233 129 84 3 63 187 31 157 1.24e-12 65.5
MS.gene70250.t1 AT1G33480 28.713 101 69 2 114 214 75 172 2.13e-12 65.1
MS.gene70250.t1 AT3G11110 40.000 70 42 0 114 183 80 149 4.03e-12 62.8
MS.gene70250.t1 AT2G42350 30.841 107 62 3 77 183 48 142 5.77e-12 63.5
MS.gene70250.t1 AT2G37580 34.483 87 51 2 97 183 104 184 7.01e-12 63.5
MS.gene70250.t1 AT5G56340 38.272 81 42 2 103 183 228 300 8.03e-12 64.3
MS.gene70250.t1 AT4G26400 42.647 68 31 2 116 183 223 282 1.02e-11 63.9
MS.gene70250.t1 AT4G26400 42.647 68 31 2 116 183 223 282 1.02e-11 63.9
MS.gene70250.t1 AT4G30370 36.620 71 44 1 114 184 89 158 1.11e-11 62.0
MS.gene70250.t1 AT1G53010 50.000 50 25 0 140 189 129 178 1.27e-11 61.6
MS.gene70250.t1 AT4G11360 35.714 84 50 2 102 183 48 129 1.74e-11 60.8
MS.gene70250.t1 AT3G20395 32.283 127 81 3 60 186 95 216 3.05e-11 61.6
MS.gene70250.t1 AT4G26580 36.709 79 44 2 113 186 256 333 3.83e-11 62.0
MS.gene70250.t1 AT4G26580 36.709 79 44 2 113 186 256 333 3.83e-11 62.0
MS.gene70250.t1 AT2G03000 52.273 44 20 1 140 183 480 522 5.55e-11 62.0
MS.gene70250.t1 AT3G47990 54.000 50 22 1 134 183 228 276 5.86e-11 61.6
MS.gene70250.t1 AT3G47990 54.000 50 22 1 134 183 212 260 6.26e-11 61.6
MS.gene70250.t1 AT3G47990 54.000 50 22 1 134 183 171 219 6.47e-11 61.2
MS.gene70250.t1 AT3G47990 54.000 50 22 1 134 183 171 219 6.47e-11 61.2
MS.gene70250.t1 AT3G47990 54.000 50 22 1 134 183 171 219 6.47e-11 61.2

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCAACCAACTCATATTT+TGG 0.110117 4.1:+8753107 MS.gene70250:CDS
AATATGAGTTGGTTGAATTT+TGG 0.155497 4.1:-8753103 None:intergenic
TCTGGCACTGGTAACAAAAT+TGG 0.215829 4.1:-8753566 None:intergenic
TGGATTGCTATAATAATTTC+TGG 0.238268 4.1:-8753584 None:intergenic
ATTGATGTTCAATTGAATTT+TGG 0.255066 4.1:-8753046 None:intergenic
CGAGTTGAATCTACCAAGTT+TGG 0.287389 4.1:+8753358 MS.gene70250:CDS
CTTGAAACTTGTCGAAAGAT+TGG 0.318725 4.1:+8753527 MS.gene70250:CDS
TGAAATCCATGGTTGCATTT+AGG 0.344720 4.1:-8753440 None:intergenic
TTTGGCTATTTGTGAAAATA+CGG 0.358119 4.1:-8753085 None:intergenic
ACCACTTGAGCACGAAGCTT+TGG 0.379749 4.1:+8753607 MS.gene70250:CDS
TTGCATTAAACTTCATCATA+AGG 0.411192 4.1:+8753198 MS.gene70250:CDS
CAACAACAATCCACCTCAAT+TGG 0.419352 4.1:+8753277 MS.gene70250:CDS
ATGTTCGTTGCATTGACAAA+TGG 0.437642 4.1:+8753462 MS.gene70250:CDS
GCTATAATAATTTCTGGCAC+TGG 0.465203 4.1:-8753578 None:intergenic
TCGGTGGAATAGCTCATTGT+AGG 0.466374 4.1:-8753338 None:intergenic
AGAGTTCACAAAGGGTGAAA+AGG 0.489010 4.1:+8753406 MS.gene70250:CDS
TTGCATTGACAAATGGCTAA+AGG 0.492896 4.1:+8753469 MS.gene70250:CDS
AAGCTTCGTGCTCAAGTGGT+TGG 0.506524 4.1:-8753604 None:intergenic
TTGGCTATTTGTGAAAATAC+GGG 0.508540 4.1:-8753084 None:intergenic
TTGTAGAATCTCACACAAGA+AGG 0.511006 4.1:+8753015 MS.gene70250:CDS
TCCAAAGCTTCGTGCTCAAG+TGG 0.522894 4.1:-8753608 None:intergenic
ATGGAGAAGTGGTGAAGTAA+AGG 0.523047 4.1:-8752985 None:intergenic
TCGAAAGATTGGTGGGTCGC+AGG 0.526432 4.1:+8753538 MS.gene70250:CDS
ATTCCTTTGATGACCAATTG+AGG 0.528975 4.1:-8753290 None:intergenic
CCACCTCAATTGGTCATCAA+AGG 0.529321 4.1:+8753287 MS.gene70250:CDS
AGAAGACATTGTCTACACTT+AGG 0.531664 4.1:-8753506 None:intergenic
ACACACTCTGTATCCAAACT+TGG 0.542352 4.1:-8753371 None:intergenic
GAAACTTGTCGAAAGATTGG+TGG 0.551880 4.1:+8753530 MS.gene70250:CDS
TATGATGAAGTTTAATGCAA+GGG 0.555043 4.1:-8753195 None:intergenic
ACGAGCTCCAAAATATGAGT+TGG 0.556220 4.1:-8753114 None:intergenic
TTATGATGAAGTTTAATGCA+AGG 0.569193 4.1:-8753196 None:intergenic
CCTTTGATGACCAATTGAGG+TGG 0.578584 4.1:-8753287 None:intergenic
TGTCTCTCAGAGTTCACAAA+GGG 0.586259 4.1:+8753398 MS.gene70250:CDS
AAACTTGTCGAAAGATTGGT+GGG 0.592454 4.1:+8753531 MS.gene70250:CDS
GAGATTCTACAAGAAGCTCA+TGG 0.595637 4.1:-8753004 None:intergenic
ACAAGAAGCTCATGGAGAAG+TGG 0.602538 4.1:-8752996 None:intergenic
CAAACTTGGTAGATTCAACT+CGG 0.621643 4.1:-8753357 None:intergenic
TGCAACGAACATGAAATCCA+TGG 0.629921 4.1:-8753451 None:intergenic
ATGTCTCTCAGAGTTCACAA+AGG 0.686490 4.1:+8753397 MS.gene70250:CDS
ACTTGGTAGATTCAACTCGG+TGG 0.705125 4.1:-8753354 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGCTACATTTAAAAATCTTA+AGG - chr4.1:8753228-8753247 None:intergenic 20.0%
!!! ATTGATGTTCAATTGAATTT+TGG - chr4.1:8753049-8753068 None:intergenic 20.0%
! TATGATGAAGTTTAATGCAA+GGG - chr4.1:8753198-8753217 None:intergenic 25.0%
! TGGATTGCTATAATAATTTC+TGG - chr4.1:8753587-8753606 None:intergenic 25.0%
! TTATGATGAAGTTTAATGCA+AGG - chr4.1:8753199-8753218 None:intergenic 25.0%
! TTGCATTAAACTTCATCATA+AGG + chr4.1:8753198-8753217 MS.gene70250:CDS 25.0%
! TTTGGCTATTTGTGAAAATA+CGG - chr4.1:8753088-8753107 None:intergenic 25.0%
!! AATTCAACCAACTCATATTT+TGG + chr4.1:8753107-8753126 MS.gene70250:CDS 25.0%
!!! AATATGAGTTGGTTGAATTT+TGG - chr4.1:8753106-8753125 None:intergenic 25.0%
TTGGCTATTTGTGAAAATAC+GGG - chr4.1:8753087-8753106 None:intergenic 30.0%
AAACTTGTCGAAAGATTGGT+GGG + chr4.1:8753531-8753550 MS.gene70250:CDS 35.0%
AGAAGACATTGTCTACACTT+AGG - chr4.1:8753509-8753528 None:intergenic 35.0%
CAAACTTGGTAGATTCAACT+CGG - chr4.1:8753360-8753379 None:intergenic 35.0%
CTTGAAACTTGTCGAAAGAT+TGG + chr4.1:8753527-8753546 MS.gene70250:CDS 35.0%
TGAAATCCATGGTTGCATTT+AGG - chr4.1:8753443-8753462 None:intergenic 35.0%
TTGTAGAATCTCACACAAGA+AGG + chr4.1:8753015-8753034 MS.gene70250:CDS 35.0%
! ATGTTCGTTGCATTGACAAA+TGG + chr4.1:8753462-8753481 MS.gene70250:CDS 35.0%
! ATTCCTTTGATGACCAATTG+AGG - chr4.1:8753293-8753312 None:intergenic 35.0%
! ATTTTGCCTAAATGCAACCA+TGG + chr4.1:8753434-8753453 MS.gene70250:CDS 35.0%
! TTGCATTGACAAATGGCTAA+AGG + chr4.1:8753469-8753488 MS.gene70250:CDS 35.0%
!! GCTATAATAATTTCTGGCAC+TGG - chr4.1:8753581-8753600 None:intergenic 35.0%
ACACACTCTGTATCCAAACT+TGG - chr4.1:8753374-8753393 None:intergenic 40.0%
ACGAGCTCCAAAATATGAGT+TGG - chr4.1:8753117-8753136 None:intergenic 40.0%
AGAGTTCACAAAGGGTGAAA+AGG + chr4.1:8753406-8753425 MS.gene70250:CDS 40.0%
ATGGAGAAGTGGTGAAGTAA+AGG - chr4.1:8752988-8753007 None:intergenic 40.0%
ATGTCTCTCAGAGTTCACAA+AGG + chr4.1:8753397-8753416 MS.gene70250:CDS 40.0%
CAACAACAATCCACCTCAAT+TGG + chr4.1:8753277-8753296 MS.gene70250:CDS 40.0%
GAAACTTGTCGAAAGATTGG+TGG + chr4.1:8753530-8753549 MS.gene70250:CDS 40.0%
GAGATTCTACAAGAAGCTCA+TGG - chr4.1:8753007-8753026 None:intergenic 40.0%
TGCAACGAACATGAAATCCA+TGG - chr4.1:8753454-8753473 None:intergenic 40.0%
TGTCTCTCAGAGTTCACAAA+GGG + chr4.1:8753398-8753417 MS.gene70250:CDS 40.0%
! CGAGTTGAATCTACCAAGTT+TGG + chr4.1:8753358-8753377 MS.gene70250:CDS 40.0%
!! TCTGGCACTGGTAACAAAAT+TGG - chr4.1:8753569-8753588 None:intergenic 40.0%
ACAAGAAGCTCATGGAGAAG+TGG - chr4.1:8752999-8753018 None:intergenic 45.0%
ACTTGGTAGATTCAACTCGG+TGG - chr4.1:8753357-8753376 None:intergenic 45.0%
CCACCTCAATTGGTCATCAA+AGG + chr4.1:8753287-8753306 MS.gene70250:CDS 45.0%
TCGGTGGAATAGCTCATTGT+AGG - chr4.1:8753341-8753360 None:intergenic 45.0%
! CCTTTGATGACCAATTGAGG+TGG - chr4.1:8753290-8753309 None:intergenic 45.0%
AAGCTTCGTGCTCAAGTGGT+TGG - chr4.1:8753607-8753626 None:intergenic 50.0%
ACCACTTGAGCACGAAGCTT+TGG + chr4.1:8753607-8753626 MS.gene70250:CDS 50.0%
TCCAAAGCTTCGTGCTCAAG+TGG - chr4.1:8753611-8753630 None:intergenic 50.0%
! TCGAAAGATTGGTGGGTCGC+AGG + chr4.1:8753538-8753557 MS.gene70250:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 8752969 8753664 8752969 ID=MS.gene70250
chr4.1 mRNA 8752969 8753664 8752969 ID=MS.gene70250.t1;Parent=MS.gene70250
chr4.1 exon 8752969 8753664 8752969 ID=MS.gene70250.t1.exon1;Parent=MS.gene70250.t1
chr4.1 CDS 8752969 8753664 8752969 ID=cds.MS.gene70250.t1;Parent=MS.gene70250.t1
Gene Sequence

>MS.gene70250

ATGTATACTTCTACTTCCTTTACTTCACCACTTCTCCATGAGCTTCTTGTAGAATCTCACACAAGAAGGTTACTCTTCCAAAATTCAATTGAACATCAATCACTTAAAATCAACTCCCGTATTTTCACAAATAGCCAAAATTCAACCAACTCATATTTTGGAGCTCGTGAATTTGATTCAAATGTTGTGATGATACTTGCAGTTCTATTATGTGCTTTTATTTGTTCCCTTGCATTAAACTTCATCATAAGGCGTGCCTTAAGATTTTTAAATGTAGCTCTCAACAACGACTCTTCTTCGAGTTCAAGCAACAACAATCCACCTCAATTGGTCATCAAAGGAATCAAGAAGAAAGCTCTCAAGAAATTTCCTACAATGAGCTATTCCACCGAGTTGAATCTACCAAGTTTGGATACAGAGTGTGTGATATGTCTCTCAGAGTTCACAAAGGGTGAAAAGGTGCGCATTTTGCCTAAATGCAACCATGGATTTCATGTTCGTTGCATTGACAAATGGCTAAAGGAACACTCATCATGTCCTAAGTGTAGACAATGTCTTCTTGAAACTTGTCGAAAGATTGGTGGGTCGCAGGTGCAACCAATTTTGTTACCAGTGCCAGAAATTATTATAGCAATCCAACCACTTGAGCACGAAGCTTTGGAACGAAACTATAGAGAAATACAAGCTAACAATTAA
Protein sequence

>MS.gene70250.t1

MYTSTSFTSPLLHELLVESHTRRLLFQNSIEHQSLKINSRIFTNSQNSTNSYFGAREFDSNVVMILAVLLCAFICSLALNFIIRRALRFLNVALNNDSSSSSSNNNPPQLVIKGIKKKALKKFPTMSYSTELNLPSLDTECVICLSEFTKGEKVRILPKCNHGFHVRCIDKWLKEHSSCPKCRQCLLETCRKIGGSQVQPILLPVPEIIIAIQPLEHEALERNYREIQANN