AlfalfaGEDB | MS.gene015624

M. sativa cultivar XinJiangDaYe / MS.gene015624

KEGG: K11096
GO: GO:0005575 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0016070 GO:0030532 GO:0032991 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0120114 GO:1901360 GO:1990904
PFAM: PF01423.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015624.t1	XP_003630987.1	100	104	0	0	1	104	5	108	2.50E-51	211.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015624.t1	Q3SZF8	84.5	103	16	0	1	103	11	113	3.0e-45	182.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015624.t1	G7LBH6	100.0	104	0	0	1	104	5	108	1.8e-51	211.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene50211	MS.gene015624	PPI
MS.gene015624	MS.gene23567	PPI
MS.gene068867	MS.gene015624	PPI
MS.gene015624	MS.gene043455	PPI
MS.gene015624	MS.gene28762	PPI
MS.gene015624	MS.gene073734	PPI
MS.gene92231	MS.gene015624	PPI
MS.gene032681	MS.gene015624	PPI
MS.gene028393	MS.gene015624	PPI
MS.gene015624	MS.gene31434	PPI
MS.gene015624	MS.gene28172	PPI
MS.gene95848	MS.gene015624	PPI
MS.gene015624	MS.gene062632	PPI
MS.gene015624	MS.gene83565	PPI
MS.gene015624	MS.gene067444	PPI
MS.gene59503	MS.gene015624	PPI
MS.gene015624	MS.gene007200	PPI
MS.gene015624	MS.gene050303	PPI
MS.gene035725	MS.gene015624	PPI
MS.gene015624	MS.gene060774	PPI
MS.gene009798	MS.gene015624	PPI
MS.gene015624	MS.gene85931	PPI
MS.gene015624	MS.gene86968	PPI
MS.gene83322	MS.gene015624	PPI
MS.gene015624	MS.gene023131	PPI
MS.gene58148	MS.gene015624	PPI
MS.gene015624	MS.gene009798	PPI
MS.gene069779	MS.gene015624	PPI
MS.gene015624	MS.gene97334	PPI
MS.gene015624	MS.gene56983	PPI
MS.gene44813	MS.gene015624	PPI
MS.gene015624	MS.gene90813	PPI
MS.gene62660	MS.gene015624	PPI
MS.gene015624	MS.gene065853	PPI
MS.gene070248	MS.gene015624	PPI
MS.gene006712	MS.gene015624	PPI
MS.gene015624	MS.gene035486	PPI
MS.gene046119	MS.gene015624	PPI
MS.gene015624	MS.gene70292	PPI
MS.gene53517	MS.gene015624	PPI
MS.gene82776	MS.gene015624	PPI
MS.gene64804	MS.gene015624	PPI
MS.gene43700	MS.gene015624	PPI
MS.gene015624	MS.gene073888	PPI
MS.gene058567	MS.gene015624	PPI
MS.gene015624	MS.gene055778	PPI
MS.gene015624	MS.gene053443	PPI
MS.gene015624	MS.gene030697	PPI
MS.gene36469	MS.gene015624	PPI
MS.gene065862	MS.gene015624	PPI
MS.gene015624	MS.gene48652	PPI
MS.gene015624	MS.gene029082	PPI
MS.gene24692	MS.gene015624	PPI
MS.gene053099	MS.gene015624	PPI
MS.gene015624	MS.gene35064	PPI
MS.gene015624	MS.gene028322	PPI
MS.gene045470	MS.gene015624	PPI
MS.gene015624	MS.gene67483	PPI
MS.gene015624	MS.gene055162	PPI
MS.gene015624	MS.gene012386	PPI
MS.gene015624	MS.gene065857	PPI
MS.gene015624	MS.gene043867	PPI
MS.gene015624	MS.gene065836	PPI
MS.gene015624	MS.gene051172	PPI
MS.gene015624	MS.gene35136	PPI
MS.gene028868	MS.gene015624	PPI
MS.gene051241	MS.gene015624	PPI
MS.gene015624	MS.gene93333	PPI
MS.gene015624	MS.gene070731	PPI
MS.gene015624	MS.gene040757	PPI
MS.gene015624	MS.gene051245	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015624.t1	MTR_8g105890	100.000	104	0	1	104	5	108	4.28e-73	212
MS.gene015624.t1	MTR_8g105890	100.000	100	0	5	104	25	124	5.04e-70	205
MS.gene015624.t1	MTR_5g097030	93.137	102	7	3	104	58	159	7.40e-65	193
MS.gene015624.t1	MTR_8g105890	100.000	82	0	23	104	1	82	4.46e-56	168

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015624.t1	AT3G62840	94.231	104	6	0	1	104	5	108	1.47e-68	201
MS.gene015624.t1	AT2G47640	94.231	104	6	0	1	104	5	108	1.47e-68	201
MS.gene015624.t1	AT2G47640	94.231	104	6	0	1	104	5	108	1.47e-68	201
MS.gene015624.t1	AT3G62840	93.333	105	6	1	1	104	5	109	5.19e-67	197
MS.gene015624.t1	AT2G47640	93.333	105	6	1	1	104	5	109	5.19e-67	197
MS.gene015624.t1	AT2G47640	93.333	105	6	1	1	104	5	109	5.19e-67	197

Find 27 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGGAGATACTAGCTTATT+TGG	0.120204	4.1:-1354030	None:intergenic
ATTCTGTCATCATTGTTCTT+AGG	0.250153	4.1:+1354003	MS.gene015624:CDS
ATGGAGATACTAGCTTATTT+GGG	0.292207	4.1:-1354029	None:intergenic
AAAAGCTTCTAGGCCGTGTC+AGG	0.374760	4.1:+1353739	MS.gene015624:CDS
TTCTTTCCTTTACCAGTCTT+AGG	0.400785	4.1:-1353924	None:intergenic
ATACCTCAGTCCACATTTCC+CGG	0.427349	4.1:-1353800	None:intergenic
ATATGGTTCTTGAAAATGTC+CGG	0.429724	4.1:+1353781	MS.gene015624:CDS
CTCAATTGTTACAGGCTGTC+GGG	0.432771	4.1:+1353524	MS.gene015624:intron
ATCAGCAAAATGTTCCTTCG+TGG	0.456450	4.1:+1353978	MS.gene015624:CDS
TAGCTTACATCTTCTTCCAT+TGG	0.480536	4.1:-1352868	None:intergenic
CAGGTGCCTAAGACTGGTAA+AGG	0.497162	4.1:+1353918	MS.gene015624:intron
TTGAAAATGTCCGGGAAATG+TGG	0.500328	4.1:+1353790	MS.gene015624:CDS
CTCATCATGAGTACAGATAG+TGG	0.509132	4.1:-1353577	None:intergenic
ATGTATCAGGTGCCTAAGAC+TGG	0.510734	4.1:+1353912	MS.gene015624:intron
GAAGGCTCAGCCAGTCAACA+AGG	0.537512	4.1:+1353947	MS.gene015624:CDS
ATGAAACGATCCTTGTTGAC+TGG	0.541044	4.1:-1353957	None:intergenic
ACTCAATTGTTACAGGCTGT+CGG	0.551091	4.1:+1353523	MS.gene015624:intron
GACTGGTAAAGGAAAGAAGA+AGG	0.552162	4.1:+1353929	MS.gene015624:CDS
TGTCGGGAAGAATGAGGAAG+AGG	0.555164	4.1:+1353540	MS.gene015624:CDS
TATGGTTCTTGAAAATGTCC+GGG	0.564509	4.1:+1353782	MS.gene015624:CDS
GTCTATCAAAAGCCCTGACA+CGG	0.582472	4.1:-1353752	None:intergenic
TGGAGATACTAGCTTATTTG+GGG	0.598626	4.1:-1354028	None:intergenic
ACAGGCTGTCGGGAAGAATG+AGG	0.617342	4.1:+1353534	MS.gene015624:intron
GAGGAAGAGGAATTCAACAC+TGG	0.639973	4.1:+1353553	MS.gene015624:CDS
AAAGCTTCTAGGCCGTGTCA+GGG	0.669612	4.1:+1353740	MS.gene015624:CDS
TGTCCGGGAAATGTGGACTG+AGG	0.699661	4.1:+1353797	MS.gene015624:CDS
TGATGACAGAATCACCACGA+AGG	0.746510	4.1:-1353992	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTTAAAAAACAG+AGG	+	chr4.1:1353378-1353397	MS.gene015624:intron	10.0%
!!!	ACTTTGACTTTAATGTTAAA+TGG	+	chr4.1:1353456-1353475	MS.gene015624:intron	20.0%
!!!	TGTTTTATCATTACATAACT+TGG	+	chr4.1:1353168-1353187	MS.gene015624:intron	20.0%
!	ATCATTACATAACTTGGTAA+AGG	+	chr4.1:1353174-1353193	MS.gene015624:intron	25.0%
!!	TCAATATGCTCTATTTGTTT+TGG	+	chr4.1:1353113-1353132	MS.gene015624:intron	25.0%
!!!	ATTTGAATTTACTTTTCTGC+TGG	+	chr4.1:1353863-1353882	MS.gene015624:intron	25.0%
	ATATGGTTCTTGAAAATGTC+CGG	+	chr4.1:1353781-1353800	MS.gene015624:CDS	30.0%
	ATGCAAATTACTTCTCTACT+TGG	+	chr4.1:1353837-1353856	MS.gene015624:intron	30.0%
	ATTCTGTCATCATTGTTCTT+AGG	+	chr4.1:1354003-1354022	MS.gene015624:CDS	30.0%
	GAAGAAAGAAAAAAGAAGAC+TGG	-	chr4.1:1353412-1353431	None:intergenic	30.0%
	TGAAATCACAATTCTGAATC+AGG	-	chr4.1:1353139-1353158	None:intergenic	30.0%
	TGTAGCTTCTGTTAAAATCA+AGG	-	chr4.1:1353020-1353039	None:intergenic	30.0%
	TGTTCTATTTAGTGCACATT+TGG	+	chr4.1:1352961-1352980	MS.gene015624:intron	30.0%
	TTTGCATTATGATCCTGATA+TGG	+	chr4.1:1352923-1352942	MS.gene015624:intron	30.0%
!	GATTTTACAAACACTTATGC+AGG	+	chr4.1:1353692-1353711	MS.gene015624:intron	30.0%
!	TTTTGATAGACACTGCAATA+TGG	+	chr4.1:1353764-1353783	MS.gene015624:CDS	30.0%
!!	ATTTTAACAGAAGCTACACT+TGG	+	chr4.1:1353022-1353041	MS.gene015624:intron	30.0%
	AAAAAAGAAGACTGGACTTC+AGG	-	chr4.1:1353404-1353423	None:intergenic	35.0%
	ACAGATATGCAATCCATATC+AGG	-	chr4.1:1352939-1352958	None:intergenic	35.0%
	ATGTTCTGCTTGTATGTATC+AGG	+	chr4.1:1353899-1353918	MS.gene015624:intron	35.0%
	ATTTGGATTGACCTTGAGTT+TGG	+	chr4.1:1352978-1352997	MS.gene015624:intron	35.0%
	CGTAACAACAAAAAGCTTCT+AGG	+	chr4.1:1353729-1353748	MS.gene015624:CDS	35.0%
	CTGACATACTCAATTGTTAC+AGG	+	chr4.1:1353516-1353535	MS.gene015624:intron	35.0%
	TACTGTGTGTTCTTCTTGAT+CGG	+	chr4.1:1353647-1353666	MS.gene015624:intron	35.0%
	TATGGTTCTTGAAAATGTCC+GGG	+	chr4.1:1353782-1353801	MS.gene015624:CDS	35.0%
	TGAGTTTGACTAAATCATGC+TGG	-	chr4.1:1353069-1353088	None:intergenic	35.0%
	TTCTTTCCTTTACCAGTCTT+AGG	-	chr4.1:1353927-1353946	None:intergenic	35.0%
!!	TGAATTTACTTTTCTGCTGG+TGG	+	chr4.1:1353866-1353885	MS.gene015624:intron	35.0%
	ACTCAATTGTTACAGGCTGT+CGG	+	chr4.1:1353523-1353542	MS.gene015624:intron	40.0%
	ATCAGCAAAATGTTCCTTCG+TGG	+	chr4.1:1353978-1353997	MS.gene015624:CDS	40.0%
	ATGAAACGATCCTTGTTGAC+TGG	-	chr4.1:1353960-1353979	None:intergenic	40.0%
	CTCATCATGAGTACAGATAG+TGG	-	chr4.1:1353580-1353599	None:intergenic	40.0%
	GACTGGTAAAGGAAAGAAGA+AGG	+	chr4.1:1353929-1353948	MS.gene015624:CDS	40.0%
	GAGTGTGAAAAACAACACAC+AGG	+	chr4.1:1353597-1353616	MS.gene015624:CDS	40.0%
	TCTTTCTTCATGCGTCACAA+TGG	+	chr4.1:1353423-1353442	MS.gene015624:intron	40.0%
	TTGAAAATGTCCGGGAAATG+TGG	+	chr4.1:1353790-1353809	MS.gene015624:CDS	40.0%
!	TGAAAGTCAGCTTTGTGTTG+TGG	+	chr4.1:1353261-1353280	MS.gene015624:intron	40.0%
	ATACCTCAGTCCACATTTCC+CGG	-	chr4.1:1353803-1353822	None:intergenic	45.0%
	ATGTATCAGGTGCCTAAGAC+TGG	+	chr4.1:1353912-1353931	MS.gene015624:intron	45.0%
	CTCAATTGTTACAGGCTGTC+GGG	+	chr4.1:1353524-1353543	MS.gene015624:intron	45.0%
	GAGGAAGAGGAATTCAACAC+TGG	+	chr4.1:1353553-1353572	MS.gene015624:CDS	45.0%
	GTCTATCAAAAGCCCTGACA+CGG	-	chr4.1:1353755-1353774	None:intergenic	45.0%
	TGATGACAGAATCACCACGA+AGG	-	chr4.1:1353995-1354014	None:intergenic	45.0%
	TGGATTGACCTTGAGTTTGG+CGG	+	chr4.1:1352981-1353000	MS.gene015624:intron	45.0%
!!	AAAAAAAAAAAAAAAAAAGA+TGG	-	chr4.1:1353365-1353384	None:intergenic	5.0%
	AAAAGCTTCTAGGCCGTGTC+AGG	+	chr4.1:1353739-1353758	MS.gene015624:CDS	50.0%
	AAAGCTTCTAGGCCGTGTCA+GGG	+	chr4.1:1353740-1353759	MS.gene015624:CDS	50.0%
	CAGGTGCCTAAGACTGGTAA+AGG	+	chr4.1:1353918-1353937	MS.gene015624:intron	50.0%
	CCAAGATTCCGCCAAACTCA+AGG	-	chr4.1:1352992-1353011	None:intergenic	50.0%
	CCTTGAGTTTGGCGGAATCT+TGG	+	chr4.1:1352989-1353008	MS.gene015624:intron	50.0%
	TGTCGGGAAGAATGAGGAAG+AGG	+	chr4.1:1353540-1353559	MS.gene015624:CDS	50.0%
	ACAGGCTGTCGGGAAGAATG+AGG	+	chr4.1:1353534-1353553	MS.gene015624:intron	55.0%
	GAAGGCTCAGCCAGTCAACA+AGG	+	chr4.1:1353947-1353966	MS.gene015624:CDS	55.0%
!	TGTCCGGGAAATGTGGACTG+AGG	+	chr4.1:1353797-1353816	MS.gene015624:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	1352871	1354037	1352871	ID=MS.gene015624
chr4.1	mRNA	1352871	1354037	1352871	ID=MS.gene015624.t1;Parent=MS.gene015624
chr4.1	exon	1352871	1352882	1352871	ID=MS.gene015624.t1.exon1;Parent=MS.gene015624.t1
chr4.1	CDS	1352871	1352882	1352871	ID=cds.MS.gene015624.t1;Parent=MS.gene015624.t1
chr4.1	exon	1353538	1353618	1353538	ID=MS.gene015624.t1.exon2;Parent=MS.gene015624.t1
chr4.1	CDS	1353538	1353618	1353538	ID=cds.MS.gene015624.t1;Parent=MS.gene015624.t1
chr4.1	exon	1353714	1353818	1353714	ID=MS.gene015624.t1.exon3;Parent=MS.gene015624.t1
chr4.1	CDS	1353714	1353818	1353714	ID=cds.MS.gene015624.t1;Parent=MS.gene015624.t1
chr4.1	exon	1353921	1354037	1353921	ID=MS.gene015624.t1.exon4;Parent=MS.gene015624.t1
chr4.1	CDS	1353921	1354037	1353921	ID=cds.MS.gene015624.t1;Parent=MS.gene015624.t1

Gene Sequence

>MS.gene015624

ATGGAAGAAGATGTAAGCTACTTATTCTTTAATGATATTTTTTATCACAAGTTTTGCATTATGATCCTGATATGGATTGCATATCTGTTATGTTCTATTTAGTGCACATTTGGATTGACCTTGAGTTTGGCGGAATCTTGGTGTCACCTTGATTTTAACAGAAGCTACACTTGGAGTTTCAACAATATTGCAGTGCCAGCATGATTTAGTCAAACTCACAGTCAAGCTAAACATTTAAGTACTCAATATGCTCTATTTGTTTTGGCCTGATTCAGAATTGTGATTTCATCATATCTATGTTTTATCATTACATAACTTGGTAAAGGATTTTTAGTTTATGATGATTTCAAAATTTAGTTTATGTTGCTAAAAAAAATGTTCTGTTAATTTTGAAAGTCAGCTTTGTGTTGTGGCTATGTTTCTTAAAAATGAAATACTAATTTGTTTCTACATTTAGTAACACTACTTTTCTTTATTGAATTTAAGTTTTGCCATCTTTTTTTTTTTTTTTTTTTTTTAAAAAACAGAGGCCTGAAGTCCAGTCTTCTTTTTTCTTTCTTCATGCGTCACAATGGTTCTTACTGCACTTTGACTTTAATGTTAAATGGCTGTTTAAAGTTTAATATGCTTTATAATCTTGTCAAGCTGACATACTCAATTGTTACAGGCTGTCGGGAAGAATGAGGAAGAGGAATTCAACACTGGTCCACTATCTGTACTCATGATGAGTGTGAAAAACAACACACAGGTATTTTTTTTATACGACACACTCATAGTACTGTGTGTTCTTCTTGATCGGTGCTCAGAATGTTTTTGATGCTGATTTTACAAACACTTATGCAGGTTCTGATCAATTGTCGTAACAACAAAAAGCTTCTAGGCCGTGTCAGGGCTTTTGATAGACACTGCAATATGGTTCTTGAAAATGTCCGGGAAATGTGGACTGAGGTATGCGACATTTTGTTTATGCAAATTACTTCTCTACTTGGTACATTTGAATTTACTTTTCTGCTGGTGGCATATGCTAAATGTTCTGCTTGTATGTATCAGGTGCCTAAGACTGGTAAAGGAAAGAAGAAGGCTCAGCCAGTCAACAAGGATCGTTTCATCAGCAAAATGTTCCTTCGTGGTGATTCTGTCATCATTGTTCTTAGGAACCCCAAATAA

Protein sequence

>MS.gene015624.t1

MEEDAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKAQPVNKDRFISKMFLRGDSVIIVLRNPK