AlfalfaGEDB | MS.gene62660

M. sativa cultivar XinJiangDaYe / MS.gene62660

KEGG: K11096
GO: GO:0005575 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0016070 GO:0030532 GO:0032991 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0120114 GO:1901360 GO:1990904
PFAM: PF01423.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62660.t1	XP_003630987.1	100	104	0	0	1	104	5	108	2.50E-51	211.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62660.t1	Q3SZF8	84.5	103	16	0	1	103	11	113	3.0e-45	182.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62660.t1	G7LBH6	100.0	104	0	0	1	104	5	108	1.8e-51	211.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056257	MS.gene62660	0.825877	3.69E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene92231	MS.gene62660	PPI
MS.gene032681	MS.gene62660	PPI
MS.gene028393	MS.gene62660	PPI
MS.gene62660	MS.gene85931	PPI
MS.gene62660	MS.gene23567	PPI
MS.gene62660	MS.gene86968	PPI
MS.gene95848	MS.gene62660	PPI
MS.gene62660	MS.gene009798	PPI
MS.gene62660	MS.gene31434	PPI
MS.gene59503	MS.gene62660	PPI
MS.gene62660	MS.gene28172	PPI
MS.gene070248	MS.gene62660	PPI
MS.gene62660	MS.gene062632	PPI
MS.gene62660	MS.gene56983	PPI
MS.gene62660	MS.gene050303	PPI
MS.gene83322	MS.gene62660	PPI
MS.gene58148	MS.gene62660	PPI
MS.gene62660	MS.gene060774	PPI
MS.gene069779	MS.gene62660	PPI
MS.gene62660	MS.gene023131	PPI
MS.gene62660	MS.gene97334	PPI
MS.gene44813	MS.gene62660	PPI
MS.gene62660	MS.gene028322	PPI
MS.gene006712	MS.gene62660	PPI
MS.gene62660	MS.gene055778	PPI
MS.gene62660	MS.gene053443	PPI
MS.gene62660	MS.gene030697	PPI
MS.gene62660	MS.gene90813	PPI
MS.gene62660	MS.gene48652	PPI
MS.gene62660	MS.gene029082	PPI
MS.gene62660	MS.gene015624	PPI
MS.gene62660	MS.gene065853	PPI
MS.gene62660	MS.gene35064	PPI
MS.gene046119	MS.gene62660	PPI
MS.gene53517	MS.gene62660	PPI
MS.gene62660	MS.gene055162	PPI
MS.gene82776	MS.gene62660	PPI
MS.gene64804	MS.gene62660	PPI
MS.gene62660	MS.gene035486	PPI
MS.gene43700	MS.gene62660	PPI
MS.gene62660	MS.gene70292	PPI
MS.gene058567	MS.gene62660	PPI
MS.gene62660	MS.gene36469	PPI
MS.gene62660	MS.gene065836	PPI
MS.gene62660	MS.gene051172	PPI
MS.gene065862	MS.gene62660	PPI
MS.gene62660	MS.gene35136	PPI
MS.gene62660	MS.gene073888	PPI
MS.gene24692	MS.gene62660	PPI
MS.gene053099	MS.gene62660	PPI
MS.gene045470	MS.gene62660	PPI
MS.gene62660	MS.gene051245	PPI
MS.gene62660	MS.gene035725	PPI
MS.gene62660	MS.gene67483	PPI
MS.gene62660	MS.gene28762	PPI
MS.gene62660	MS.gene073734	PPI
MS.gene62660	MS.gene012386	PPI
MS.gene62660	MS.gene065857	PPI
MS.gene028868	MS.gene62660	PPI
MS.gene051241	MS.gene62660	PPI
MS.gene62660	MS.gene043867	PPI
MS.gene62660	MS.gene043455	PPI
MS.gene62660	MS.gene83565	PPI
MS.gene62660	MS.gene067444	PPI
MS.gene62660	MS.gene93333	PPI
MS.gene50211	MS.gene62660	PPI
MS.gene62660	MS.gene007200	PPI
MS.gene62660	MS.gene070731	PPI
MS.gene068867	MS.gene62660	PPI
MS.gene62660	MS.gene040757	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62660.t1	MTR_8g105890	100.000	104	0	1	104	5	108	4.28e-73	212
MS.gene62660.t1	MTR_8g105890	100.000	100	0	5	104	25	124	5.04e-70	205
MS.gene62660.t1	MTR_5g097030	93.137	102	7	3	104	58	159	7.40e-65	193
MS.gene62660.t1	MTR_8g105890	100.000	82	0	23	104	1	82	4.46e-56	168

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62660.t1	AT3G62840	94.231	104	6	0	1	104	5	108	1.47e-68	201
MS.gene62660.t1	AT2G47640	94.231	104	6	0	1	104	5	108	1.47e-68	201
MS.gene62660.t1	AT2G47640	94.231	104	6	0	1	104	5	108	1.47e-68	201
MS.gene62660.t1	AT3G62840	93.333	105	6	1	1	104	5	109	5.19e-67	197
MS.gene62660.t1	AT2G47640	93.333	105	6	1	1	104	5	109	5.19e-67	197
MS.gene62660.t1	AT2G47640	93.333	105	6	1	1	104	5	109	5.19e-67	197

Find 27 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGGAGATACTAGCTTATT+TGG	0.120204	4.2:-1104308	None:intergenic
ATTCTGTCATCATTGTTCTT+AGG	0.279548	4.2:+1104281	MS.gene62660:CDS
ATGGAGATACTAGCTTATTT+GGG	0.292207	4.2:-1104307	None:intergenic
AAAAGCTTCTAGGCCGTGTC+AGG	0.374760	4.2:+1104017	MS.gene62660:CDS
TTCTTTCCTTTACCAGTCTT+AGG	0.400785	4.2:-1104202	None:intergenic
ATACCTCAGTCCACATTTCC+CGG	0.427349	4.2:-1104078	None:intergenic
ATATGGTTCTTGAAAATGTC+CGG	0.429724	4.2:+1104059	MS.gene62660:CDS
CTCAATTGTTACAGGCTGTC+GGG	0.432771	4.2:+1103802	MS.gene62660:intron
ATCAGCAAAATGTTCCTTCG+TGG	0.434985	4.2:+1104256	MS.gene62660:CDS
TAGCTTACATCTTCTTCCAT+TGG	0.480536	4.2:-1103148	None:intergenic
CAGGTGCCTAAGACTGGTAA+AGG	0.497162	4.2:+1104196	MS.gene62660:intron
TTGAAAATGTCCGGGAAATG+TGG	0.500328	4.2:+1104068	MS.gene62660:CDS
CTCATCATGAGTACAGATAG+TGG	0.509132	4.2:-1103855	None:intergenic
ATGTATCAGGTGCCTAAGAC+TGG	0.510734	4.2:+1104190	MS.gene62660:intron
GAAGGCTCAGCCAGTCAACA+AGG	0.537512	4.2:+1104225	MS.gene62660:CDS
ATGAAACGATCCTTGTTGAC+TGG	0.541044	4.2:-1104235	None:intergenic
ACTCAATTGTTACAGGCTGT+CGG	0.551091	4.2:+1103801	MS.gene62660:intron
GACTGGTAAAGGAAAGAAGA+AGG	0.552162	4.2:+1104207	MS.gene62660:CDS
TGTCGGGAAGAATGAGGAAG+AGG	0.555164	4.2:+1103818	MS.gene62660:CDS
TATGGTTCTTGAAAATGTCC+GGG	0.564509	4.2:+1104060	MS.gene62660:CDS
GTCTATCAAAAGCCCTGACA+CGG	0.582472	4.2:-1104030	None:intergenic
TGGAGATACTAGCTTATTTG+GGG	0.598626	4.2:-1104306	None:intergenic
ACAGGCTGTCGGGAAGAATG+AGG	0.617342	4.2:+1103812	MS.gene62660:intron
GAGGAAGAGGAATTCAACAC+TGG	0.639973	4.2:+1103831	MS.gene62660:CDS
AAAGCTTCTAGGCCGTGTCA+GGG	0.669612	4.2:+1104018	MS.gene62660:CDS
TGTCCGGGAAATGTGGACTG+AGG	0.699661	4.2:+1104075	MS.gene62660:CDS
TGATGACAGAATCTCCACGA+AGG	0.746510	4.2:-1104270	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTTAAAAAACAG+AGG	+	chr4.2:1103656-1103675	MS.gene62660:intron	10.0%
!!!	ACTTTGACTTTAATGTTAAA+TGG	+	chr4.2:1103734-1103753	MS.gene62660:intron	20.0%
!!!	TGTTTTATCATTACATAACT+TGG	+	chr4.2:1103446-1103465	MS.gene62660:intron	20.0%
!	ATCATTACATAACTTGGTAA+AGG	+	chr4.2:1103452-1103471	MS.gene62660:intron	25.0%
!	TGTATCTTCTGTTAAAATCA+AGG	-	chr4.2:1103300-1103319	None:intergenic	25.0%
!!	TCAATATGCTCTATTTGTTT+TGG	+	chr4.2:1103393-1103412	MS.gene62660:intron	25.0%
!!!	ATTTGAATTTACTTTTCTGC+TGG	+	chr4.2:1104141-1104160	MS.gene62660:intron	25.0%
!!!	ATTTTAACAGAAGATACACT+TGG	+	chr4.2:1103302-1103321	MS.gene62660:intron	25.0%
	ATATGGTTCTTGAAAATGTC+CGG	+	chr4.2:1104059-1104078	MS.gene62660:CDS	30.0%
	ATGCAAATTACTTCTCTACT+TGG	+	chr4.2:1104115-1104134	MS.gene62660:intron	30.0%
	ATTCTGTCATCATTGTTCTT+AGG	+	chr4.2:1104281-1104300	MS.gene62660:CDS	30.0%
	GAAGAAAGAAAAAAGAAGAC+TGG	-	chr4.2:1103690-1103709	None:intergenic	30.0%
	TGAAATCACAATTCTGAATC+AGG	-	chr4.2:1103419-1103438	None:intergenic	30.0%
	TGTTCTATTTAGTGCACATT+TGG	+	chr4.2:1103241-1103260	MS.gene62660:intron	30.0%
	TTTGCATTATGATCCTGATA+TGG	+	chr4.2:1103203-1103222	MS.gene62660:intron	30.0%
!	GATTTTACAAACACTTATGC+AGG	+	chr4.2:1103970-1103989	MS.gene62660:intron	30.0%
!	TTTTGATAGACACTGCAATA+TGG	+	chr4.2:1104042-1104061	MS.gene62660:CDS	30.0%
	AAAAAAGAAGACTGGACTTC+AGG	-	chr4.2:1103682-1103701	None:intergenic	35.0%
	ACAGATATGCAATCCATATC+AGG	-	chr4.2:1103219-1103238	None:intergenic	35.0%
	ATGTTCTGCTTGTATGTATC+AGG	+	chr4.2:1104177-1104196	MS.gene62660:intron	35.0%
	ATTTGGATTGACCTTGAGTT+TGG	+	chr4.2:1103258-1103277	MS.gene62660:intron	35.0%
	CGTAACAACAAAAAGCTTCT+AGG	+	chr4.2:1104007-1104026	MS.gene62660:CDS	35.0%
	CTGACATACTCAATTGTTAC+AGG	+	chr4.2:1103794-1103813	MS.gene62660:intron	35.0%
	TACTGTGTGTTCTTCTTGAT+CGG	+	chr4.2:1103925-1103944	MS.gene62660:intron	35.0%
	TATGGTTCTTGAAAATGTCC+GGG	+	chr4.2:1104060-1104079	MS.gene62660:CDS	35.0%
	TGAGTTTGACTAAATCATGC+TGG	-	chr4.2:1103349-1103368	None:intergenic	35.0%
	TTCTTTCCTTTACCAGTCTT+AGG	-	chr4.2:1104205-1104224	None:intergenic	35.0%
!!	TGAATTTACTTTTCTGCTGG+TGG	+	chr4.2:1104144-1104163	MS.gene62660:intron	35.0%
	ACTCAATTGTTACAGGCTGT+CGG	+	chr4.2:1103801-1103820	MS.gene62660:intron	40.0%
	ATCAGCAAAATGTTCCTTCG+TGG	+	chr4.2:1104256-1104275	MS.gene62660:CDS	40.0%
	ATGAAACGATCCTTGTTGAC+TGG	-	chr4.2:1104238-1104257	None:intergenic	40.0%
	CTCATCATGAGTACAGATAG+TGG	-	chr4.2:1103858-1103877	None:intergenic	40.0%
	GACTGGTAAAGGAAAGAAGA+AGG	+	chr4.2:1104207-1104226	MS.gene62660:CDS	40.0%
	GAGTGTGAAAAACAACACAC+AGG	+	chr4.2:1103875-1103894	MS.gene62660:CDS	40.0%
	TCTTTCTTCATGCGTCACAA+TGG	+	chr4.2:1103701-1103720	MS.gene62660:intron	40.0%
	TTGAAAATGTCCGGGAAATG+TGG	+	chr4.2:1104068-1104087	MS.gene62660:CDS	40.0%
!	TGAAAGTCAGCTTTGTGTTG+TGG	+	chr4.2:1103539-1103558	MS.gene62660:intron	40.0%
	ATACCTCAGTCCACATTTCC+CGG	-	chr4.2:1104081-1104100	None:intergenic	45.0%
	ATGTATCAGGTGCCTAAGAC+TGG	+	chr4.2:1104190-1104209	MS.gene62660:intron	45.0%
	CTCAATTGTTACAGGCTGTC+GGG	+	chr4.2:1103802-1103821	MS.gene62660:intron	45.0%
	GAGGAAGAGGAATTCAACAC+TGG	+	chr4.2:1103831-1103850	MS.gene62660:CDS	45.0%
	GTCTATCAAAAGCCCTGACA+CGG	-	chr4.2:1104033-1104052	None:intergenic	45.0%
	TGATGACAGAATCTCCACGA+AGG	-	chr4.2:1104273-1104292	None:intergenic	45.0%
	TGGATTGACCTTGAGTTTGG+CGG	+	chr4.2:1103261-1103280	MS.gene62660:intron	45.0%
!!	AAAAAAAAAAAAAAAAAAGA+TGG	-	chr4.2:1103643-1103662	None:intergenic	5.0%
	AAAAGCTTCTAGGCCGTGTC+AGG	+	chr4.2:1104017-1104036	MS.gene62660:CDS	50.0%
	AAAGCTTCTAGGCCGTGTCA+GGG	+	chr4.2:1104018-1104037	MS.gene62660:CDS	50.0%
	CAGGTGCCTAAGACTGGTAA+AGG	+	chr4.2:1104196-1104215	MS.gene62660:intron	50.0%
	CCAAGATTCCGCCAAACTCA+AGG	-	chr4.2:1103272-1103291	None:intergenic	50.0%
	CCTTGAGTTTGGCGGAATCT+TGG	+	chr4.2:1103269-1103288	MS.gene62660:intron	50.0%
	TGTCGGGAAGAATGAGGAAG+AGG	+	chr4.2:1103818-1103837	MS.gene62660:CDS	50.0%
	ACAGGCTGTCGGGAAGAATG+AGG	+	chr4.2:1103812-1103831	MS.gene62660:intron	55.0%
	GAAGGCTCAGCCAGTCAACA+AGG	+	chr4.2:1104225-1104244	MS.gene62660:CDS	55.0%
!	TGTCCGGGAAATGTGGACTG+AGG	+	chr4.2:1104075-1104094	MS.gene62660:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	1103151	1104315	1103151	ID=MS.gene62660
chr4.2	mRNA	1103151	1104315	1103151	ID=MS.gene62660.t1;Parent=MS.gene62660
chr4.2	exon	1103151	1103162	1103151	ID=MS.gene62660.t1.exon1;Parent=MS.gene62660.t1
chr4.2	CDS	1103151	1103162	1103151	ID=cds.MS.gene62660.t1;Parent=MS.gene62660.t1
chr4.2	exon	1103816	1103896	1103816	ID=MS.gene62660.t1.exon2;Parent=MS.gene62660.t1
chr4.2	CDS	1103816	1103896	1103816	ID=cds.MS.gene62660.t1;Parent=MS.gene62660.t1
chr4.2	exon	1103992	1104096	1103992	ID=MS.gene62660.t1.exon3;Parent=MS.gene62660.t1
chr4.2	CDS	1103992	1104096	1103992	ID=cds.MS.gene62660.t1;Parent=MS.gene62660.t1
chr4.2	exon	1104199	1104315	1104199	ID=MS.gene62660.t1.exon4;Parent=MS.gene62660.t1
chr4.2	CDS	1104199	1104315	1104199	ID=cds.MS.gene62660.t1;Parent=MS.gene62660.t1

Gene Sequence

>MS.gene62660

ATGGAAGAAGATGTAAGCTACTTATTCTTTAATGATATTTTTTATCACAAGTTTTGCATTATGATCCTGATATGGATTGCATATCTGTTATGTTCTATTTAGTGCACATTTGGATTGACCTTGAGTTTGGCGGAATCTTGGTGTCACCTTGATTTTAACAGAAGATACACTTGGAGTTTCAACAATATTGCAGTGCCAGCATGATTTAGTCAAACTCACAGTCAAGCTAAACATTTAAGTACTCAATATGCTCTATTTGTTTTGGCCTGATTCAGAATTGTGATTTCATCATATCTGTTTTATCATTACATAACTTGGTAAAGGATTTTTAGTTTATGATGATTTCAAAATTTAGTTTATGTTGCTAAAAAAAATGTTCTGTTAATTTTGAAAGTCAGCTTTGTGTTGTGGCTATGTTTCTTAAAAATGAAATACTAATTTGTTTCTACATTTAGTAACACTACTTTTCTTTATTGAATTTAAGTTTTGCCATCTTTTTTTTTTTTTTTTTTTTTTAAAAAACAGAGGCCTGAAGTCCAGTCTTCTTTTTTCTTTCTTCATGCGTCACAATGGTTCTTACTGCACTTTGACTTTAATGTTAAATGGCTGTTTAAAGTTTAATATGCTTTATAATCTTGTCAAGCTGACATACTCAATTGTTACAGGCTGTCGGGAAGAATGAGGAAGAGGAATTCAACACTGGTCCACTATCTGTACTCATGATGAGTGTGAAAAACAACACACAGGTATTTTTTTTATACGACACACTCATAGTACTGTGTGTTCTTCTTGATCGGTGCTCAGAATGTTTTTGATGCTGATTTTACAAACACTTATGCAGGTTCTGATCAATTGTCGTAACAACAAAAAGCTTCTAGGCCGTGTCAGGGCTTTTGATAGACACTGCAATATGGTTCTTGAAAATGTCCGGGAAATGTGGACTGAGGTATGCGACATTTTGTTTATGCAAATTACTTCTCTACTTGGTACATTTGAATTTACTTTTCTGCTGGTGGCATATGCTAAATGTTCTGCTTGTATGTATCAGGTGCCTAAGACTGGTAAAGGAAAGAAGAAGGCTCAGCCAGTCAACAAGGATCGTTTCATCAGCAAAATGTTCCTTCGTGGAGATTCTGTCATCATTGTTCTTAGGAACCCCAAATAA

Protein sequence

>MS.gene62660.t1

MEEDAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKAQPVNKDRFISKMFLRGDSVIIVLRNPK