AlfalfaGEDB | MS.gene058567

M. sativa cultivar XinJiangDaYe / MS.gene058567

KEGG: K11096
GO: GO:0005575 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0016070 GO:0030532 GO:0032991 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0120114 GO:1901360 GO:1990904
PFAM: PF01423.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058567.t1	XP_003630987.1	100	108	0	0	1	108	1	108	7.30E-54	219.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058567.t1	Q3SZF8	84.5	103	16	0	5	107	11	113	2.4e-45	182.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058567.t1	G7LBH6	100.0	108	0	0	1	108	1	108	5.3e-54	219.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene058567	MS.gene92231	PPI
MS.gene058567	MS.gene043455	PPI
MS.gene058567	MS.gene67483	PPI
MS.gene058567	MS.gene028393	PPI
MS.gene83322	MS.gene058567	PPI
MS.gene058567	MS.gene28762	PPI
MS.gene058567	MS.gene012386	PPI
MS.gene058567	MS.gene95848	PPI
MS.gene058567	MS.gene065857	PPI
MS.gene058567	MS.gene043867	PPI
MS.gene069779	MS.gene058567	PPI
MS.gene058567	MS.gene83565	PPI
MS.gene058567	MS.gene067444	PPI
MS.gene058567	MS.gene93333	PPI
MS.gene058567	MS.gene070731	PPI
MS.gene058567	MS.gene040757	PPI
MS.gene44813	MS.gene058567	PPI
MS.gene058567	MS.gene051245	PPI
MS.gene070248	MS.gene058567	PPI
MS.gene058567	MS.gene069779	PPI
MS.gene006712	MS.gene058567	PPI
MS.gene058567	MS.gene85931	PPI
MS.gene058567	MS.gene23567	PPI
MS.gene058567	MS.gene86968	PPI
MS.gene058567	MS.gene073734	PPI
MS.gene046119	MS.gene058567	PPI
MS.gene058567	MS.gene009798	PPI
MS.gene058567	MS.gene31434	PPI
MS.gene53517	MS.gene058567	PPI
MS.gene058567	MS.gene28172	PPI
MS.gene82776	MS.gene058567	PPI
MS.gene43700	MS.gene058567	PPI
MS.gene058567	MS.gene062632	PPI
MS.gene058567	MS.gene007200	PPI
MS.gene058567	MS.gene050303	PPI
MS.gene065862	MS.gene058567	PPI
MS.gene058567	MS.gene060774	PPI
MS.gene24692	MS.gene058567	PPI
MS.gene053099	MS.gene058567	PPI
MS.gene045470	MS.gene058567	PPI
MS.gene058567	MS.gene023131	PPI
MS.gene058567	MS.gene97334	PPI
MS.gene058567	MS.gene62660	PPI
MS.gene028868	MS.gene058567	PPI
MS.gene058567	MS.gene055778	PPI
MS.gene051241	MS.gene058567	PPI
MS.gene058567	MS.gene053443	PPI
MS.gene058567	MS.gene56983	PPI
MS.gene058567	MS.gene90813	PPI
MS.gene058567	MS.gene48652	PPI
MS.gene058567	MS.gene029082	PPI
MS.gene058567	MS.gene015624	PPI
MS.gene058567	MS.gene065853	PPI
MS.gene058567	MS.gene35064	PPI
MS.gene058567	MS.gene028322	PPI
MS.gene058567	MS.gene58148	PPI
MS.gene058567	MS.gene032681	PPI
MS.gene068867	MS.gene058567	PPI
MS.gene058567	MS.gene055162	PPI
MS.gene058567	MS.gene64804	PPI
MS.gene058567	MS.gene035486	PPI
MS.gene058567	MS.gene70292	PPI
MS.gene058567	MS.gene36469	PPI
MS.gene058567	MS.gene065836	PPI
MS.gene058567	MS.gene051172	PPI
MS.gene058567	MS.gene35136	PPI
MS.gene95848	MS.gene058567	PPI
MS.gene058567	MS.gene073888	PPI
MS.gene59503	MS.gene058567	PPI
MS.gene058567	MS.gene030697	PPI
MS.gene058567	MS.gene035725	PPI
MS.gene50211	MS.gene058567	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058567.t1	MTR_8g105890	100.000	108	0	0	1	108	1	108	2.20e-76	221
MS.gene058567.t1	MTR_8g105890	100.000	100	0	0	9	108	25	124	6.28e-70	205
MS.gene058567.t1	MTR_5g097030	91.509	106	8	1	3	108	55	159	4.52e-65	194
MS.gene058567.t1	MTR_8g105890	100.000	82	0	0	27	108	1	82	5.79e-56	168

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058567.t1	AT3G62840	93.519	108	7	0	1	108	1	108	3.43e-71	207
MS.gene058567.t1	AT2G47640	93.519	108	7	0	1	108	1	108	3.43e-71	207
MS.gene058567.t1	AT2G47640	93.519	108	7	0	1	108	1	108	3.43e-71	207
MS.gene058567.t1	AT3G62840	92.661	109	7	1	1	108	1	109	1.35e-69	203
MS.gene058567.t1	AT2G47640	92.661	109	7	1	1	108	1	109	1.35e-69	203
MS.gene058567.t1	AT2G47640	92.661	109	7	1	1	108	1	109	1.35e-69	203

Find 27 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGGAGATACTAGCTTATT+TGG	0.120204	4.4:+1697029	None:intergenic
ATTCTGTCATCATTGTTCTT+AGG	0.279548	4.4:-1697056	MS.gene058567:CDS
ATGGAGATACTAGCTTATTT+GGG	0.292207	4.4:+1697030	None:intergenic
TTCTTTCCTTTACCAGTCTT+AGG	0.400785	4.4:+1697135	None:intergenic
ATATGGTTCTTGAAAACGTC+CGG	0.429724	4.4:-1697273	MS.gene058567:CDS
CTCAATTGTTACAGGCTGTC+GGG	0.432771	4.4:-1697530	MS.gene058567:intron
ATCAGCAAAATGTTCCTTCG+TGG	0.434985	4.4:-1697081	MS.gene058567:CDS
ATACCTCAGTCCACATTTCC+CGG	0.461626	4.4:+1697254	None:intergenic
TAGCTTACATCTTCTTCCAT+TGG	0.480536	4.4:+1698193	None:intergenic
CAGGTGCCTAAGACTGGTAA+AGG	0.497162	4.4:-1697141	MS.gene058567:CDS
TTACATCTTCTTCCATTGGC+CGG	0.497216	4.4:+1698197	None:intergenic
CTCATCATGAGTACAGATAG+TGG	0.509132	4.4:+1697477	None:intergenic
ATGTATCAGGTGCCTAAGAC+TGG	0.510734	4.4:-1697147	MS.gene058567:intron
GAAGGCTCAGCCAGTCAACA+AGG	0.537512	4.4:-1697112	MS.gene058567:CDS
ATGAAACGATCCTTGTTGAC+TGG	0.541044	4.4:+1697102	None:intergenic
TTGAAAACGTCCGGGAAATG+TGG	0.543592	4.4:-1697264	MS.gene058567:CDS
ACTCAATTGTTACAGGCTGT+CGG	0.551091	4.4:-1697531	MS.gene058567:intron
TTCTTCTTGAAGCCGGCCAA+TGG	0.551208	4.4:-1698209	MS.gene058567:intron
GACTGGTAAAGGAAAGAAGA+AGG	0.552162	4.4:-1697130	MS.gene058567:CDS
TGTCGGGAAGAATGAGGAAG+AGG	0.555164	4.4:-1697514	MS.gene058567:CDS
GTCTATCAAAAGCCCTGACA+CGG	0.582472	4.4:+1697302	None:intergenic
TATGGTTCTTGAAAACGTCC+GGG	0.592732	4.4:-1697272	MS.gene058567:CDS
TGGAGATACTAGCTTATTTG+GGG	0.598626	4.4:+1697031	None:intergenic
ACAGGCTGTCGGGAAGAATG+AGG	0.617342	4.4:-1697520	MS.gene058567:intron
GAGGAAGAGGAATTCAACAC+TGG	0.639973	4.4:-1697501	MS.gene058567:CDS
CGTCCGGGAAATGTGGACTG+AGG	0.692273	4.4:-1697257	MS.gene058567:intron
TGATGACAGAATCTCCACGA+AGG	0.746510	4.4:+1697067	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTAAAAAAACAG+AGG	-	chr4.4:1697680-1697699	MS.gene058567:intron	10.0%
!!!	ACTTTGACTTTAATGTTAAA+TGG	-	chr4.4:1697758-1697777	MS.gene058567:intron	20.0%
!!!	TGTTTTATCATTACATAACT+TGG	-	chr4.4:1697463-1697482	MS.gene058567:CDS	20.0%
!	ATCATTACATAACTTGGTAA+AGG	-	chr4.4:1697469-1697488	MS.gene058567:CDS	25.0%
!!	TCAATATGCTCTATTTGTTT+TGG	-	chr4.4:1697408-1697427	MS.gene058567:intron	25.0%
!!!	ATTTGAATTTACTTTTCTGC+TGG	-	chr4.4:1698160-1698179	MS.gene058567:intron	25.0%
	AAGAAAGAAAAAAGAAGACC+GGG	+	chr4.4:1697713-1697732	None:intergenic	30.0%
	ATGAAAATTGCTTCTCTACT+TGG	-	chr4.4:1698134-1698153	MS.gene058567:intron	30.0%
	ATTCTGTCATCATTGTTCTT+AGG	-	chr4.4:1698300-1698319	MS.gene058567:intron	30.0%
	GAAGAAAGAAAAAAGAAGAC+CGG	+	chr4.4:1697714-1697733	None:intergenic	30.0%
	TGAAATCACAATTCTGAATC+AGG	+	chr4.4:1697434-1697453	None:intergenic	30.0%
	TGTAGCTTCTGTTAAAATCA+AGG	+	chr4.4:1697315-1697334	None:intergenic	30.0%
	TGTTCTATTTAGTGCACATT+TGG	-	chr4.4:1697256-1697275	MS.gene058567:intron	30.0%
	TTTGCATTATGATCCTGATA+TGG	-	chr4.4:1697218-1697237	MS.gene058567:intron	30.0%
!	CGTAACAACAAAAAGCTTTT+AGG	-	chr4.4:1698031-1698050	MS.gene058567:intron	30.0%
!	GATTTTACAAACACTTATGC+AGG	-	chr4.4:1697994-1698013	MS.gene058567:intron	30.0%
!	TTGATTTTTCTTCTTGAAGC+CGG	-	chr4.4:1697140-1697159	MS.gene058567:CDS	30.0%
!	TTTTGATAGACACTGCAATA+TGG	-	chr4.4:1698066-1698085	MS.gene058567:intron	30.0%
!!	ATTTTAACAGAAGCTACACT+TGG	-	chr4.4:1697317-1697336	MS.gene058567:CDS	30.0%
	ACAGATATGCAATCCATATC+AGG	+	chr4.4:1697234-1697253	None:intergenic	35.0%
	ATATGGTTCTTGAAAACGTC+CGG	-	chr4.4:1698083-1698102	MS.gene058567:intron	35.0%
	ATGTTCTGCTTGTATGTATC+AGG	-	chr4.4:1698196-1698215	MS.gene058567:intron	35.0%
	ATTTGGATTGACCTTGAGTT+TGG	-	chr4.4:1697273-1697292	MS.gene058567:CDS	35.0%
	CTGACATACTCAATTGTTAC+AGG	-	chr4.4:1697818-1697837	MS.gene058567:intron	35.0%
	TACTGTGTGTTCTTCTTGAT+CGG	-	chr4.4:1697949-1697968	MS.gene058567:intron	35.0%
	TAGCTTACATCTTCTTCCAT+TGG	+	chr4.4:1697166-1697185	None:intergenic	35.0%
	TGAGTTTGACTAAATCATGC+TGG	+	chr4.4:1697364-1697383	None:intergenic	35.0%
	TTCTTTCCTTTACCAGTCTT+AGG	+	chr4.4:1698224-1698243	None:intergenic	35.0%
!!	TGAATTTACTTTTCTGCTGG+TGG	-	chr4.4:1698163-1698182	MS.gene058567:intron	35.0%
	ACTCAATTGTTACAGGCTGT+CGG	-	chr4.4:1697825-1697844	MS.gene058567:intron	40.0%
	ATCAGCAAAATGTTCCTTCG+TGG	-	chr4.4:1698275-1698294	MS.gene058567:intron	40.0%
	ATGAAACGATCCTTGTTGAC+TGG	+	chr4.4:1698257-1698276	None:intergenic	40.0%
	CTCATCATGAGTACAGATAG+TGG	+	chr4.4:1697882-1697901	None:intergenic	40.0%
	GACTGGTAAAGGAAAGAAGA+AGG	-	chr4.4:1698226-1698245	MS.gene058567:intron	40.0%
	GAGTGTGAAAAACAACACAC+AGG	-	chr4.4:1697899-1697918	MS.gene058567:intron	40.0%
	TATGGTTCTTGAAAACGTCC+GGG	-	chr4.4:1698084-1698103	MS.gene058567:intron	40.0%
	TCTTTCTTCATGCGTCACAA+TGG	-	chr4.4:1697725-1697744	MS.gene058567:intron	40.0%
	TGAGGTATGTATTCTCTTGC+TGG	-	chr4.4:1697045-1697064	MS.gene058567:CDS	40.0%
	TTACATCTTCTTCCATTGGC+CGG	+	chr4.4:1697162-1697181	None:intergenic	40.0%
!	TGAAAGTCAGCTTTGTGTTG+TGG	-	chr4.4:1697556-1697575	MS.gene058567:intron	40.0%
	AAAAAAGAAGACCGGGCTTC+AGG	+	chr4.4:1697706-1697725	None:intergenic	45.0%
	ATACCTCAGTCCACATTTCC+CGG	+	chr4.4:1698105-1698124	None:intergenic	45.0%
	ATGTATCAGGTGCCTAAGAC+TGG	-	chr4.4:1698209-1698228	MS.gene058567:intron	45.0%
	CTCAATTGTTACAGGCTGTC+GGG	-	chr4.4:1697826-1697845	MS.gene058567:intron	45.0%
	GAGGAAGAGGAATTCAACAC+TGG	-	chr4.4:1697855-1697874	MS.gene058567:intron	45.0%
	GTCTATCAAAAGCCCTGACA+CGG	+	chr4.4:1698057-1698076	None:intergenic	45.0%
	TGATGACAGAATCTCCACGA+AGG	+	chr4.4:1698292-1698311	None:intergenic	45.0%
	TGGATTGACCTTGAGTTTGG+CGG	-	chr4.4:1697276-1697295	MS.gene058567:CDS	45.0%
	TTGAAAACGTCCGGGAAATG+TGG	-	chr4.4:1698092-1698111	MS.gene058567:intron	45.0%
!	AAAAGCTTTTAGGCCGTGTC+AGG	-	chr4.4:1698041-1698060	MS.gene058567:intron	45.0%
!	AAAGCTTTTAGGCCGTGTCA+GGG	-	chr4.4:1698042-1698061	MS.gene058567:intron	45.0%
!!	AAAAAAAAAAAAAAAAAAGA+TGG	+	chr4.4:1697660-1697679	None:intergenic	5.0%
	AAAAACAGAGGCCTGAAGCC+CGG	-	chr4.4:1697692-1697711	MS.gene058567:intron	50.0%
	CAGGTGCCTAAGACTGGTAA+AGG	-	chr4.4:1698215-1698234	MS.gene058567:intron	50.0%
	CCAAGATTCCGCCAAACTCA+AGG	+	chr4.4:1697287-1697306	None:intergenic	50.0%
	CCTTGAGTTTGGCGGAATCT+TGG	-	chr4.4:1697284-1697303	MS.gene058567:CDS	50.0%
	TGTCGGGAAGAATGAGGAAG+AGG	-	chr4.4:1697842-1697861	MS.gene058567:intron	50.0%
	TTCTTCTTGAAGCCGGCCAA+TGG	-	chr4.4:1697147-1697166	MS.gene058567:intron	50.0%
	ACAGGCTGTCGGGAAGAATG+AGG	-	chr4.4:1697836-1697855	MS.gene058567:intron	55.0%
	GAAGGCTCAGCCAGTCAACA+AGG	-	chr4.4:1698244-1698263	MS.gene058567:intron	55.0%
!	CGTCCGGGAAATGTGGACTG+AGG	-	chr4.4:1698099-1698118	MS.gene058567:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	1697044	1698334	1697044	ID=MS.gene058567
chr4.4	mRNA	1697044	1698334	1697044	ID=MS.gene058567.t1;Parent=MS.gene058567
chr4.4	exon	1698330	1698334	1698330	ID=MS.gene058567.t1.exon1;Parent=MS.gene058567.t1
chr4.4	CDS	1698330	1698334	1698330	ID=cds.MS.gene058567.t1;Parent=MS.gene058567.t1
chr4.4	exon	1698201	1698219	1698201	ID=MS.gene058567.t1.exon2;Parent=MS.gene058567.t1
chr4.4	CDS	1698201	1698219	1698201	ID=cds.MS.gene058567.t1;Parent=MS.gene058567.t1
chr4.4	exon	1697458	1697538	1697458	ID=MS.gene058567.t1.exon3;Parent=MS.gene058567.t1
chr4.4	CDS	1697458	1697538	1697458	ID=cds.MS.gene058567.t1;Parent=MS.gene058567.t1
chr4.4	exon	1697258	1697362	1697258	ID=MS.gene058567.t1.exon4;Parent=MS.gene058567.t1
chr4.4	CDS	1697258	1697362	1697258	ID=cds.MS.gene058567.t1;Parent=MS.gene058567.t1
chr4.4	exon	1697044	1697160	1697044	ID=MS.gene058567.t1.exon5;Parent=MS.gene058567.t1
chr4.4	CDS	1697044	1697160	1697044	ID=cds.MS.gene058567.t1;Parent=MS.gene058567.t1

Gene Sequence

>MS.gene058567

ATGAGGTATGTATTCTCTTGCTGGTTATTTTGTGAAATTGAGATATATTATTATCATTATTTTTAAATTTTCAGTTGTCTAACATTTTTTGTTTTCTTGATTTTTCTTCTTGAAGCCGGCCAATGGAAGAAGATGTAAGCTACTTATTCTTTAATGATATTTTTTATCACAAGTTTTGCATTATGATCCTGATATGGATTGCATATCTGTTATGTTCTATTTAGTGCACATTTGGATTGACCTTGAGTTTGGCGGAATCTTGGTGTCACCTTGATTTTAACAGAAGCTACACTTGGAGTTTCAACAATATTGCAGTGCCAGCATGATTTAGTCAAACTCACAGTCAAGCTAAACATTTAAGTACTCAATATGCTCTATTTGTTTTGGCCTGATTCAGAATTGTGATTTCATCATATCTATGTTTTATCATTACATAACTTGGTAAAGGATTTTTAGTTTATGATGATTTCAAAATTTAGTTTATGTTGCTAAAAAAAATGTTCTGTTAATTTTGAAAGTCAGCTTTGTGTTGTGGCTATGTTTCTTAAAAATGAAATACTAATTTGTTTCTACATTTAGTAACACTACTTTTCTTTATTGAATTTAAGTTTTGCCATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAACAGAGGCCTGAAGCCCGGTCTTCTTTTTTCTTTCTTCATGCGTCACAATGGTTCTTACTGCACTTTGACTTTAATGTTAAATGGCTGTTTAAAGTTTAATATGCTTTAAAATCTTGTCAAGCTGACATACTCAATTGTTACAGGCTGTCGGGAAGAATGAGGAAGAGGAATTCAACACTGGTCCACTATCTGTACTCATGATGAGTGTGAAAAACAACACACAGGTATTTTTTTTATACGACACACTCATAGTACTGTGTGTTCTTCTTGATCGGTGCTCAGAATGTTTTTGATGCTGATTTTACAAACACTTATGCAGGTTCTGATCAATTGTCGTAACAACAAAAAGCTTTTAGGCCGTGTCAGGGCTTTTGATAGACACTGCAATATGGTTCTTGAAAACGTCCGGGAAATGTGGACTGAGGTATATTTTGTTTATGAAAATTGCTTCTCTACTTGGTACATTTGAATTTACTTTTCTGCTGGTGGCATATGCTAAATGTTCTGCTTGTATGTATCAGGTGCCTAAGACTGGTAAAGGAAAGAAGAAGGCTCAGCCAGTCAACAAGGATCGTTTCATCAGCAAAATGTTCCTTCGTGGAGATTCTGTCATCATTGTTCTTAGGAACCCCAAATAA

Protein sequence

>MS.gene058567.t1

MSRPMEEDAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKAQPVNKDRFISKMFLRGDSVIIVLRNPK