AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45771


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45771.t1 MTR_8g037933 91.848 184 9 1 1 184 1 178 2.34e-111 316
MS.gene45771.t1 MTR_0695s0010 86.111 36 5 0 35 70 18 53 5.43e-16 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCATTATGTCCTTCT+AGG 0.088496 8.3:-58717115 None:intergenic
TTTGGAATAGGTTTGTAAAT+TGG 0.202275 8.3:-58717500 None:intergenic
CTTTGGAATAGGCTTGTAAA+TGG 0.212935 8.3:-58717309 None:intergenic
TTTGGAATAGGCTTGTAAAT+TGG 0.232414 8.3:-58717356 None:intergenic
TTTGGAATAGGCTTGTAAAT+TGG 0.232414 8.3:-58717404 None:intergenic
TTTGGAATAGGCTTGTAAAT+TGG 0.232414 8.3:-58717452 None:intergenic
GGAATTGGTTTCTTGAATAT+TGG 0.237384 8.3:-58717197 None:intergenic
TTATAGATTGGAACGTATTT+TGG 0.246929 8.3:-58717266 None:intergenic
TTTGGTGTAGGTTTGTAAAT+TGG 0.267204 8.3:-58717248 None:intergenic
ATTGGAACAGGCTTGTAAAT+TGG 0.276520 8.3:-58717386 None:intergenic
ATTGGAACAGGCTTGTAAAT+TGG 0.276520 8.3:-58717434 None:intergenic
ATTGGAACAGGCTTGTAAAT+TGG 0.276520 8.3:-58717482 None:intergenic
TTGTGAATAGGGTTGTAAAA+TGG 0.314774 8.3:-58717218 None:intergenic
ATTGGAATTACCTTTGGAAT+AGG 0.316743 8.3:-58717368 None:intergenic
ATTGGAATTACCTTTGGAAT+AGG 0.316743 8.3:-58717416 None:intergenic
ATTGGAATTACCTTTGGAAT+AGG 0.316743 8.3:-58717464 None:intergenic
ATTGGAATGTCTTTGTGAAT+AGG 0.325208 8.3:-58717230 None:intergenic
TTGGAATGTCTTTGTGAATA+GGG 0.326122 8.3:-58717229 None:intergenic
CAATATGCAAAGGCCACTTT+TGG 0.329589 8.3:+58716238 MS.gene45771:CDS
ATGGGAATTTCCTTTGGAAT+AGG 0.348694 8.3:-58717512 None:intergenic
ACTGGAATTACCTTTGGAAT+AGG 0.360911 8.3:-58717320 None:intergenic
ATTGGAACAGGCTTGTAAAC+TGG 0.363527 8.3:-58717338 None:intergenic
ATAGGCTTGTAAATGGGAAT+TGG 0.370906 8.3:-58717302 None:intergenic
CAAAAGTGGCCTTTGCATAT+TGG 0.376190 8.3:-58716237 None:intergenic
ATAGGGTTGTAAAATGGAAT+TGG 0.379124 8.3:-58717212 None:intergenic
TTTACAATGGGAATTTCCTT+TGG 0.389183 8.3:-58717518 None:intergenic
GTAGCAAGAAATGATCCTTC+AGG 0.392442 8.3:+58716314 MS.gene45771:CDS
ATAGGTTTGTAAATTGGAAC+AGG 0.400844 8.3:-58717494 None:intergenic
TTGTAAACTGGAATTACCTT+TGG 0.413189 8.3:-58717326 None:intergenic
TTGTAAATTGGAATTACCTT+TGG 0.416670 8.3:-58717374 None:intergenic
TTGTAAATTGGAATTACCTT+TGG 0.416670 8.3:-58717422 None:intergenic
TTGTAAATTGGAATTACCTT+TGG 0.416670 8.3:-58717470 None:intergenic
TAATGGATTGGCTTTACAAT+GGG 0.425861 8.3:-58717530 None:intergenic
ATAGGCTTGTAAATTGGAAC+AGG 0.440807 8.3:-58717350 None:intergenic
ATAGGCTTGTAAATTGGAAC+AGG 0.440807 8.3:-58717398 None:intergenic
ATAGGCTTGTAAATTGGAAC+AGG 0.440807 8.3:-58717446 None:intergenic
ATGGGAATTGGTTTGTGGAT+AGG 0.448083 8.3:-58717290 None:intergenic
GCTTCCCATCTGTGTTCACT+TGG 0.449323 8.3:-58717064 None:intergenic
GCATGATAATATTAATGGAT+TGG 0.453668 8.3:-58717542 None:intergenic
ACAACAAAGACAAACCTAGA+AGG 0.456489 8.3:+58717101 MS.gene45771:CDS
TTGTGGATAGGCTTATAGAT+TGG 0.461177 8.3:-58717278 None:intergenic
TGTAAATGGGAATTGGTTTG+TGG 0.491139 8.3:-58717295 None:intergenic
TTTGGAATAGGCTTGTAAAT+GGG 0.493642 8.3:-58717308 None:intergenic
GGAAACCAAGTGAACACAGA+TGG 0.546430 8.3:+58717059 MS.gene45771:intron
TTAATGGATTGGCTTTACAA+TGG 0.550457 8.3:-58717531 None:intergenic
CATCTAAAACACAAACTCAA+AGG 0.559830 8.3:+58717158 MS.gene45771:CDS
AATTTACAAACCTATTCCAA+AGG 0.560996 8.3:+58717502 MS.gene45771:CDS
CATTTACAAGCCTATTCCAA+AGG 0.578514 8.3:+58717310 MS.gene45771:CDS
GAAACCAAGTGAACACAGAT+GGG 0.582541 8.3:+58717060 MS.gene45771:CDS
GGAACCCAGAAGGCCAAAAG+TGG 0.583318 8.3:-58716251 None:intergenic
AATTTACAAGCCTATTCCAA+AGG 0.584966 8.3:+58717358 MS.gene45771:CDS
AATTTACAAGCCTATTCCAA+AGG 0.584966 8.3:+58717406 MS.gene45771:CDS
AATTTACAAGCCTATTCCAA+AGG 0.584966 8.3:+58717454 MS.gene45771:CDS
AAGGTGACAAGGAGCAACAA+AGG 0.600910 8.3:-58716272 None:intergenic
TAGCATAACAGAAGGTGACA+AGG 0.606710 8.3:-58716283 None:intergenic
GAGCAACAAAGGAACCCAGA+AGG 0.618628 8.3:-58716261 None:intergenic
GAAAAGCTTCCAATATGCAA+AGG 0.633003 8.3:+58716228 MS.gene45771:CDS
AACAGAAGTAGCATAACAGA+AGG 0.639211 8.3:-58716291 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAATAAAATTTTATTTGA+AGG + chr8.3:58716686-58716705 MS.gene45771:intron 10.0%
!! AAAAAAGAGAGAGAAATTTA+TGG + chr8.3:58716483-58716502 MS.gene45771:intron 20.0%
!! AATGAAGAAGAAAAATTCAA+AGG + chr8.3:58717128-58717147 MS.gene45771:CDS 20.0%
!! ACATTTATATTTCCCTTATT+TGG + chr8.3:58716459-58716478 MS.gene45771:intron 20.0%
! AATTTACAAACCTATTCCAA+AGG + chr8.3:58717502-58717521 MS.gene45771:CDS 25.0%
! ACCATTTAAACATGACTTTA+AGG - chr8.3:58716543-58716562 None:intergenic 25.0%
! AGTAGTACATATTCATAGAT+TGG + chr8.3:58716409-58716428 MS.gene45771:intron 25.0%
! GTAGTACATATTCATAGATT+GGG + chr8.3:58716410-58716429 MS.gene45771:intron 25.0%
! TCCTTAAAGTCATGTTTAAA+TGG + chr8.3:58716539-58716558 MS.gene45771:intron 25.0%
! TTACTCGATTTATAAACTCT+AGG - chr8.3:58717021-58717040 None:intergenic 25.0%
! TTGTAAATTGGAATTACCTT+TGG - chr8.3:58717377-58717396 None:intergenic 25.0%
!! TTATAGATTGGAACGTATTT+TGG - chr8.3:58717269-58717288 None:intergenic 25.0%
!! TTTGGAATAGGTTTGTAAAT+TGG - chr8.3:58717503-58717522 None:intergenic 25.0%
!!! ATTTTTGTTTACTCTTGGTT+TGG + chr8.3:58716435-58716454 MS.gene45771:intron 25.0%
!!! CTCTCTTTTTTACCAAATAA+GGG - chr8.3:58716474-58716493 None:intergenic 25.0%
!!! GTTTCTTGAATATTGGTTTT+TGG - chr8.3:58717193-58717212 None:intergenic 25.0%
!!! TCTCTCTTTTTTACCAAATA+AGG - chr8.3:58716475-58716494 None:intergenic 25.0%
AAACAAAACAAACACACACT+TGG + chr8.3:58716980-58716999 MS.gene45771:intron 30.0%
AATTTACAAGCCTATTCCAA+AGG + chr8.3:58717358-58717377 MS.gene45771:CDS 30.0%
ACTATCATCATAATCACACA+AGG - chr8.3:58716587-58716606 None:intergenic 30.0%
ATAGGGTTGTAAAATGGAAT+TGG - chr8.3:58717215-58717234 None:intergenic 30.0%
ATAGGTTTGTAAATTGGAAC+AGG - chr8.3:58717497-58717516 None:intergenic 30.0%
ATTGGAATGTCTTTGTGAAT+AGG - chr8.3:58717233-58717252 None:intergenic 30.0%
TATTATACTCACAAACCTGA+AGG - chr8.3:58716332-58716351 None:intergenic 30.0%
TTGGAATGTCTTTGTGAATA+GGG - chr8.3:58717232-58717251 None:intergenic 30.0%
TTGTAAACTGGAATTACCTT+TGG - chr8.3:58717329-58717348 None:intergenic 30.0%
TTGTGAATAGGGTTGTAAAA+TGG - chr8.3:58717221-58717240 None:intergenic 30.0%
TTTACAATGGGAATTTCCTT+TGG - chr8.3:58717521-58717540 None:intergenic 30.0%
! CATCTAAAACACAAACTCAA+AGG + chr8.3:58717158-58717177 MS.gene45771:CDS 30.0%
! GGAATTGGTTTCTTGAATAT+TGG - chr8.3:58717200-58717219 None:intergenic 30.0%
! TAATTTTGCATTTCTTGTGC+AGG + chr8.3:58717038-58717057 MS.gene45771:intron 30.0%
! TTTGGAATAGGCTTGTAAAT+GGG - chr8.3:58717311-58717330 None:intergenic 30.0%
! TTTGGAATAGGCTTGTAAAT+TGG - chr8.3:58717311-58717330 None:intergenic 30.0%
! TTTGGTGTAGGTTTGTAAAT+TGG - chr8.3:58717251-58717270 None:intergenic 30.0%
!! ATTGGAATTACCTTTGGAAT+AGG - chr8.3:58717371-58717390 None:intergenic 30.0%
!! TAATGGATTGGCTTTACAAT+GGG - chr8.3:58717533-58717552 None:intergenic 30.0%
!! TTAATGGATTGGCTTTACAA+TGG - chr8.3:58717534-58717553 None:intergenic 30.0%
!!! GGGTTATTTTTGTTTACTCT+TGG + chr8.3:58716430-58716449 MS.gene45771:intron 30.0%
AACAGAAGTAGCATAACAGA+AGG - chr8.3:58716294-58716313 None:intergenic 35.0%
ACAACAAAGACAAACCTAGA+AGG + chr8.3:58717101-58717120 MS.gene45771:CDS 35.0%
ATAGGCTTGTAAATGGGAAT+TGG - chr8.3:58717305-58717324 None:intergenic 35.0%
ATAGGCTTGTAAATTGGAAC+AGG - chr8.3:58717353-58717372 None:intergenic 35.0%
ATTGGAACAGGCTTGTAAAT+TGG - chr8.3:58717389-58717408 None:intergenic 35.0%
CATTTACAAGCCTATTCCAA+AGG + chr8.3:58717310-58717329 MS.gene45771:CDS 35.0%
CTTCTTCATTATGTCCTTCT+AGG - chr8.3:58717118-58717137 None:intergenic 35.0%
GAAAAGCTTCCAATATGCAA+AGG + chr8.3:58716228-58716247 MS.gene45771:CDS 35.0%
GGTTCAAGAAGAATTGAACT+CGG + chr8.3:58716822-58716841 MS.gene45771:intron 35.0%
TTGTGGATAGGCTTATAGAT+TGG - chr8.3:58717281-58717300 None:intergenic 35.0%
!! ACTGGAATTACCTTTGGAAT+AGG - chr8.3:58717323-58717342 None:intergenic 35.0%
!! ATGGGAATTTCCTTTGGAAT+AGG - chr8.3:58717515-58717534 None:intergenic 35.0%
!! CTTTGGAATAGGCTTGTAAA+TGG - chr8.3:58717312-58717331 None:intergenic 35.0%
!! TGTAAATGGGAATTGGTTTG+TGG - chr8.3:58717298-58717317 None:intergenic 35.0%
!!! ATTGGAACGTATTTTGGTGT+AGG - chr8.3:58717263-58717282 None:intergenic 35.0%
ATTGGAACAGGCTTGTAAAC+TGG - chr8.3:58717341-58717360 None:intergenic 40.0%
CAAAAGTGGCCTTTGCATAT+TGG - chr8.3:58716240-58716259 None:intergenic 40.0%
GAAACCAAGTGAACACAGAT+GGG + chr8.3:58717060-58717079 MS.gene45771:CDS 40.0%
GTAGCAAGAAATGATCCTTC+AGG + chr8.3:58716314-58716333 MS.gene45771:CDS 40.0%
TAGCATAACAGAAGGTGACA+AGG - chr8.3:58716286-58716305 None:intergenic 40.0%
!! ATGGGAATTGGTTTGTGGAT+AGG - chr8.3:58717293-58717312 None:intergenic 40.0%
!! CAATATGCAAAGGCCACTTT+TGG + chr8.3:58716238-58716257 MS.gene45771:CDS 40.0%
AAGGTGACAAGGAGCAACAA+AGG - chr8.3:58716275-58716294 None:intergenic 45.0%
ACACACTTGGCCATTAAGCT+AGG + chr8.3:58716993-58717012 MS.gene45771:intron 45.0%
ACTCTAGGTGCCTAGCTTAA+TGG - chr8.3:58717006-58717025 None:intergenic 45.0%
CAAACCAATACACACGACAC+AGG + chr8.3:58716790-58716809 MS.gene45771:intron 45.0%
GGAAACCAAGTGAACACAGA+TGG + chr8.3:58717059-58717078 MS.gene45771:intron 45.0%
!!! ATAATTTAGTTTTTAATAAA+AGG - chr8.3:58716736-58716755 None:intergenic 5.0%
AGTGCCTGTGTCGTGTGTAT+TGG - chr8.3:58716797-58716816 None:intergenic 50.0%
GAGCAACAAAGGAACCCAGA+AGG - chr8.3:58716264-58716283 None:intergenic 50.0%
GCTTCCCATCTGTGTTCACT+TGG - chr8.3:58717067-58717086 None:intergenic 50.0%
!!! AAAGGCCACTTTTGGCCTTC+TGG + chr8.3:58716246-58716265 MS.gene45771:CDS 50.0%
!!! AAGGCCACTTTTGGCCTTCT+GGG + chr8.3:58716247-58716266 MS.gene45771:CDS 50.0%
ACACGACACAGGCACTACGT+TGG + chr8.3:58716801-58716820 MS.gene45771:intron 55.0%
GGAACCCAGAAGGCCAAAAG+TGG - chr8.3:58716254-58716273 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 58716224 58717553 58716224 ID=MS.gene45771
chr8.3 mRNA 58716224 58717553 58716224 ID=MS.gene45771.t1;Parent=MS.gene45771
chr8.3 exon 58716224 58716335 58716224 ID=MS.gene45771.t1.exon1;Parent=MS.gene45771.t1
chr8.3 CDS 58716224 58716335 58716224 ID=cds.MS.gene45771.t1;Parent=MS.gene45771.t1
chr8.3 exon 58717060 58717553 58717060 ID=MS.gene45771.t1.exon2;Parent=MS.gene45771.t1
chr8.3 CDS 58717060 58717553 58717060 ID=cds.MS.gene45771.t1;Parent=MS.gene45771.t1
Gene Sequence

>MS.gene45771

ATGAGAAAAGCTTCCAATATGCAAAGGCCACTTTTGGCCTTCTGGGTTCCTTTGTTGCTCCTTGTCACCTTCTGTTATGCTACTTCTGTTGTAGCAAGAAATGATCCTTCAGGTTTGTGAGTATAATATTCATTTTGATATTGACATATAAATGTACAATATATGTACACAATATATATAGTAGTAGTAGTACATATTCATAGATTGGGTTATTTTTGTTTACTCTTGGTTTGGTACATTTATATTTCCCTTATTTGGTAAAAAAGAGAGAGAAATTTATGGTGTTAAAAATGACTTGAAATTGAAAATATTAGCTCCTTAAAGTCATGTTTAAATGGTCTTTTTTTAACTTCACTTTTTCCTTGTGTGATTATGATGATAGTAAAGTAAAATAAAATTTAATTTTAAAAGCATGTTTAATTAAAAGTGAAAACATATAGTTTTTGTACTGAGCAGAAAATTTTGAATAAAATTTTATTTGAAGGTGATATTTCTTTTATTGTAATTCTCCTTTTATTAAAAACTAAATTATTTGTAAATTAAGAAACTAATTAATAGTATACATTCAAACCAATACACACGACACAGGCACTACGTTGGTTCAAGAAGAATTGAACTCGGATCTCTTAAATAAATCTAACATTGTGAATGAAAAAATATGATTGAGAGAGAAAACTTCATGATCAGTCAAATTTCGTAACAGAGATTAGTCATAGAAAAGTTGATAGATATTTCGCAAAAATAAGATTGTTACAAAAACAAAACAAACACACACTTGGCCATTAAGCTAGGCACCTAGAGTTTATAAATCGAGTAATTTTGCATTTCTTGTGCAGGAAACCAAGTGAACACAGATGGGAAGCTTGCAAATGAAGAGACAACAAAGACAAACCTAGAAGGACATAATGAAGAAGAAAAATTCAAAGGATTTTTTCATCTAAAACACAAACTCAAAGGATATTTTCACCAAAAACCAATATTCAAGAAACCAATTCCATTTTACAACCCTATTCACAAAGACATTCCAATTTACAAACCTACACCAAAATACGTTCCAATCTATAAGCCTATCCACAAACCAATTCCCATTTACAAGCCTATTCCAAAGGTAATTCCAGTTTACAAGCCTGTTCCAATTTACAAGCCTATTCCAAAGGTAATTCCAATTTACAAGCCTGTTCCAATTTACAAGCCTATTCCAAAGGTAATTCCAATTTACAAGCCTGTTCCAATTTACAAGCCTATTCCAAAGGTAATTCCAATTTACAAGCCTGTTCCAATTTACAAACCTATTCCAAAGGAAATTCCCATTGTAAAGCCAATCCATTAA

Protein sequence

>MS.gene45771.t1

MRKASNMQRPLLAFWVPLLLLVTFCYATSVVARNDPSGNQVNTDGKLANEETTKTNLEGHNEEEKFKGFFHLKHKLKGYFHQKPIFKKPIPFYNPIHKDIPIYKPTPKYVPIYKPIHKPIPIYKPIPKVIPVYKPVPIYKPIPKVIPIYKPVPIYKPIPKVIPIYKPVPIYKPIPKVIPIYKPVPIYKPIPKEIPIVKPIH