AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46500.t1 MTR_7g107200 31.579 342 198 7 10 322 43 377 2.17e-52 177
MS.gene46500.t1 MTR_8g011730 33.803 284 146 8 38 295 75 342 1.02e-39 143
MS.gene46500.t1 MTR_1g042160 31.338 284 147 8 37 319 72 308 3.83e-37 135
MS.gene46500.t1 MTR_7g107170 41.259 143 81 2 177 319 124 263 3.64e-30 115
MS.gene46500.t1 MTR_7g107160 29.642 307 174 9 19 321 177 445 1.29e-28 115
MS.gene46500.t1 MTR_2g034780 31.319 182 115 3 125 306 84 255 1.23e-23 98.2
MS.gene46500.t1 MTR_3g079840 41.237 97 55 2 10 104 85 181 1.32e-15 78.2
MS.gene46500.t1 MTR_3g079840 41.237 97 55 2 10 104 86 182 1.32e-15 78.2
MS.gene46500.t1 MTR_5g086700 37.736 106 64 2 1 104 81 186 2.12e-15 77.4
MS.gene46500.t1 MTR_5g086700 37.736 106 64 2 1 104 81 186 2.20e-15 77.4
MS.gene46500.t1 MTR_1g041240 38.144 97 58 2 10 104 86 182 3.22e-14 73.9
MS.gene46500.t1 MTR_1g041240 38.144 97 58 2 10 104 87 183 3.22e-14 73.9
MS.gene46500.t1 MTR_1g041240 38.144 97 58 2 10 104 85 181 3.50e-14 73.9
MS.gene46500.t1 MTR_5g091450 33.333 99 63 3 10 105 62 160 2.31e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46500.t1 AT3G11910 42.268 97 54 2 10 104 83 179 1.09e-16 81.6
MS.gene46500.t1 AT3G11910 42.268 97 54 2 10 104 83 179 1.09e-16 81.6
MS.gene46500.t1 AT3G11910 42.268 97 54 2 10 104 84 180 1.10e-16 81.6
MS.gene46500.t1 AT3G11910 42.268 97 54 2 10 104 84 180 1.10e-16 81.6
MS.gene46500.t1 AT5G06600 40.206 97 56 2 10 104 85 181 2.97e-16 80.1
MS.gene46500.t1 AT5G06600 40.206 97 56 2 10 104 84 180 3.13e-16 80.1
MS.gene46500.t1 AT5G06600 40.206 97 56 2 10 104 85 181 3.22e-16 80.1

Find 59 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGAATTTATTGAAATTT+AGG 0.097481 1.1:-42326947 None:intergenic
TTTAGGTGTGAAACCTTATT+TGG 0.176545 1.1:+42327478 MS.gene46500:CDS
TAGCATTAACATTCATTTCT+TGG 0.196289 1.1:-42327643 None:intergenic
TCACAAGATACCCTTGTTTA+TGG 0.211988 1.1:-42326971 None:intergenic
TTGCTTGCTCTTTCCAAATA+AGG 0.215332 1.1:-42327491 None:intergenic
GATCTGTTAGAGATGAATTT+TGG 0.232734 1.1:-42327070 None:intergenic
ATTAAGACAATTTATAGATT+TGG 0.265024 1.1:+42327184 MS.gene46500:CDS
CCTTATGTTAAATCTGCTTT+AGG 0.292555 1.1:+42327461 MS.gene46500:CDS
CAATGGGCGTTTATTTCATT+AGG 0.313100 1.1:+42327314 MS.gene46500:CDS
GATGTTGAACCTGATGTTCT+TGG 0.313287 1.1:-42327122 None:intergenic
AAGAACAAGAACATTGTAAA+AGG 0.329093 1.1:+42326482 MS.gene46500:CDS
CCATTAAATACAACGTAAAA+TGG 0.335460 1.1:-42326385 None:intergenic
ATCCCGTTTGTTTCGACAAT+GGG 0.347875 1.1:+42327298 MS.gene46500:CDS
GAAGAAGCTTAGGGATGAAT+TGG 0.365012 1.1:+42327559 MS.gene46500:CDS
ACAGGATAAAGTTGTTGCTT+TGG 0.378179 1.1:+42327526 MS.gene46500:CDS
AAGCAAATTGAAGAAATTAC+AGG 0.378860 1.1:+42327508 MS.gene46500:CDS
GCTGATTCTTTACCACCATA+TGG 0.384239 1.1:+42326410 MS.gene46500:CDS
ATGAATGTTAATGCTACAAT+AGG 0.395264 1.1:+42327650 MS.gene46500:CDS
CCTTGAATCTCTCCTTCATC+TGG 0.397555 1.1:-42327149 None:intergenic
CGCCCATTGTCGAAACAAAC+GGG 0.404272 1.1:-42327300 None:intergenic
ATATTAAAATGAAGAAGCTT+AGG 0.428129 1.1:+42327549 MS.gene46500:CDS
TAAACAATCTTAAGAGTTTG+TGG 0.445086 1.1:+42327393 MS.gene46500:CDS
TGGTCAACATCCCTCCTTAG+TGG 0.451823 1.1:-42326365 None:intergenic
GGCTTATCAGAAATCAGAAA+TGG 0.452452 1.1:+42327617 MS.gene46500:CDS
AGATCAATCTTCAGATGAAA+GGG 0.455070 1.1:+42327088 MS.gene46500:CDS
CATCGGAATTCGACTTGAGT+TGG 0.457368 1.1:+42327432 MS.gene46500:CDS
ACGCCCATTGTCGAAACAAA+CGG 0.462214 1.1:-42327301 None:intergenic
GTTAGTTCTCGTTAATCAGC+TGG 0.462338 1.1:+42326457 MS.gene46500:CDS
GAGACTCAGCAAAAGTTCAA+TGG 0.462872 1.1:+42326897 MS.gene46500:intron
ATATGTCTTCATCATGTGAT+AGG 0.463059 1.1:+42326327 MS.gene46500:CDS
CCTAAAGCAGATTTAACATA+AGG 0.466436 1.1:-42327461 None:intergenic
ACAATTAGTCCAAGAACATC+AGG 0.475342 1.1:+42327113 MS.gene46500:CDS
CAGATCAATCTTCAGATGAA+AGG 0.479808 1.1:+42327087 MS.gene46500:CDS
TCAATGGAGGATATAGAAGC+TGG 0.483484 1.1:+42326913 MS.gene46500:CDS
CCAGATGAAGGAGAGATTCA+AGG 0.494194 1.1:+42327149 MS.gene46500:CDS
GAGTTTGAAGTTGCAAGGAA+AGG 0.497249 1.1:+42327596 MS.gene46500:CDS
AAACAATCTTAAGAGTTTGT+GGG 0.500829 1.1:+42327394 MS.gene46500:CDS
TATTAAAATGAAGAAGCTTA+GGG 0.501449 1.1:+42327550 MS.gene46500:CDS
AGACAATTTATAGATTTGGA+TGG 0.504986 1.1:+42327188 MS.gene46500:CDS
ATTCTTTACCACCATATGGA+TGG 0.506704 1.1:+42326414 MS.gene46500:CDS
TTCTTTACCACCATATGGAT+GGG 0.526863 1.1:+42326415 MS.gene46500:CDS
GTGTTTCTATCCCATCCATA+TGG 0.542297 1.1:-42326425 None:intergenic
AATCCCGTTTGTTTCGACAA+TGG 0.547890 1.1:+42327297 MS.gene46500:CDS
AAGCAGAGTTTGAAGTTGCA+AGG 0.552990 1.1:+42327591 MS.gene46500:CDS
ATTTGACTTTGTATCAAAGA+CGG 0.558711 1.1:-42327269 None:intergenic
CAATGGAGGATATAGAAGCT+GGG 0.563913 1.1:+42326914 MS.gene46500:CDS
AGATCCTTGTGCATCCACTA+AGG 0.569109 1.1:+42326351 MS.gene46500:CDS
AACGAGAACTAACTTGAAGA+AGG 0.575362 1.1:-42326447 None:intergenic
TCAACATCTAGTCCAGATGA+AGG 0.586033 1.1:+42327137 MS.gene46500:CDS
AAATTCATTGACCATAAACA+AGG 0.592420 1.1:+42326960 MS.gene46500:CDS
TCCTTGTGCATCCACTAAGG+AGG 0.614099 1.1:+42326354 MS.gene46500:CDS
GGAGGAACTAGCAAAATCAT+CGG 0.617394 1.1:+42327415 MS.gene46500:CDS
CCTTGTGCATCCACTAAGGA+GGG 0.618988 1.1:+42326355 MS.gene46500:CDS
CAATCTTAAGAGTTTGTGGG+AGG 0.629922 1.1:+42327397 MS.gene46500:CDS
CCCTCCTTAGTGGATGCACA+AGG 0.633723 1.1:-42326355 None:intergenic
TTTCTATCCCATCCATATGG+TGG 0.636196 1.1:-42326422 None:intergenic
ACTCAGCAAAAGTTCAATGG+AGG 0.650485 1.1:+42326900 MS.gene46500:CDS
AATGGAGGATATAGAAGCTG+GGG 0.652315 1.1:+42326915 MS.gene46500:CDS
AATTCATTGACCATAAACAA+GGG 0.668373 1.1:+42326961 MS.gene46500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAAATATTATCATTAG+AGG - chr1.1:42326833-42326852 None:intergenic 15.0%
!! ATTAAGACAATTTATAGATT+TGG + chr1.1:42327184-42327203 MS.gene46500:CDS 15.0%
!! CAATGAATTTATTGAAATTT+AGG - chr1.1:42326950-42326969 None:intergenic 15.0%
!! ATAAATGACAAAACTAGTAA+AGG - chr1.1:42326532-42326551 None:intergenic 20.0%
!! ATATTAAAATGAAGAAGCTT+AGG + chr1.1:42327549-42327568 MS.gene46500:CDS 20.0%
!! TAAATGACAAAACTAGTAAA+GGG - chr1.1:42326531-42326550 None:intergenic 20.0%
!! TATTAAAATGAAGAAGCTTA+GGG + chr1.1:42327550-42327569 MS.gene46500:CDS 20.0%
! AAACAATCTTAAGAGTTTGT+GGG + chr1.1:42327394-42327413 MS.gene46500:CDS 25.0%
! AAATGACAAAACTAGTAAAG+GGG - chr1.1:42326530-42326549 None:intergenic 25.0%
! AAATTCATTGACCATAAACA+AGG + chr1.1:42326960-42326979 MS.gene46500:CDS 25.0%
! AAGAACAAGAACATTGTAAA+AGG + chr1.1:42326482-42326501 MS.gene46500:CDS 25.0%
! AAGCAAATTGAAGAAATTAC+AGG + chr1.1:42327508-42327527 MS.gene46500:CDS 25.0%
! AATTCATTGACCATAAACAA+GGG + chr1.1:42326961-42326980 MS.gene46500:CDS 25.0%
! AGACAATTTATAGATTTGGA+TGG + chr1.1:42327188-42327207 MS.gene46500:CDS 25.0%
! ATGAACAGTTTGATCTTAAT+AGG + chr1.1:42326659-42326678 MS.gene46500:intron 25.0%
! ATGAATGTTAATGCTACAAT+AGG + chr1.1:42327650-42327669 MS.gene46500:CDS 25.0%
! CCATTAAATACAACGTAAAA+TGG - chr1.1:42326388-42326407 None:intergenic 25.0%
! TAAACAATCTTAAGAGTTTG+TGG + chr1.1:42327393-42327412 MS.gene46500:CDS 25.0%
! TAGCATTAACATTCATTTCT+TGG - chr1.1:42327646-42327665 None:intergenic 25.0%
!! ATTTGACTTTGTATCAAAGA+CGG - chr1.1:42327272-42327291 None:intergenic 25.0%
!! ATTTTAGACTTCTAGATGAA+AGG + chr1.1:42326750-42326769 MS.gene46500:intron 25.0%
!! CCATTTTACGTTGTATTTAA+TGG + chr1.1:42326385-42326404 MS.gene46500:CDS 25.0%
!! GAATTGATGTCATGAATTTT+TGG - chr1.1:42327044-42327063 None:intergenic 25.0%
!! TTTTAGACTTCTAGATGAAA+GGG + chr1.1:42326751-42326770 MS.gene46500:intron 25.0%
AAGATCTAAAGGCTCATATT+TGG + chr1.1:42326625-42326644 MS.gene46500:intron 30.0%
AGATCAATCTTCAGATGAAA+GGG + chr1.1:42327088-42327107 MS.gene46500:CDS 30.0%
ATATGTCTTCATCATGTGAT+AGG + chr1.1:42326327-42326346 MS.gene46500:CDS 30.0%
CCTAAAGCAGATTTAACATA+AGG - chr1.1:42327464-42327483 None:intergenic 30.0%
CCTTATGTTAAATCTGCTTT+AGG + chr1.1:42327461-42327480 MS.gene46500:CDS 30.0%
TTTAGGTGTGAAACCTTATT+TGG + chr1.1:42327478-42327497 MS.gene46500:CDS 30.0%
! GATCTGTTAGAGATGAATTT+TGG - chr1.1:42327073-42327092 None:intergenic 30.0%
!!! TAGAGATGAATTTTGGTTCA+AGG - chr1.1:42327066-42327085 None:intergenic 30.0%
AAAGGGGAGAATGAAGATAA+GGG - chr1.1:42326514-42326533 None:intergenic 35.0%
AACGAGAACTAACTTGAAGA+AGG - chr1.1:42326450-42326469 None:intergenic 35.0%
ACAATTAGTCCAAGAACATC+AGG + chr1.1:42327113-42327132 MS.gene46500:CDS 35.0%
ATTCTTTACCACCATATGGA+TGG + chr1.1:42326414-42326433 MS.gene46500:CDS 35.0%
CAATGGGCGTTTATTTCATT+AGG + chr1.1:42327314-42327333 MS.gene46500:CDS 35.0%
CAGATCAATCTTCAGATGAA+AGG + chr1.1:42327087-42327106 MS.gene46500:CDS 35.0%
CCTACCGAATCATTAAGTTA+GGG + chr1.1:42326592-42326611 MS.gene46500:intron 35.0%
GGCTTATCAGAAATCAGAAA+TGG + chr1.1:42327617-42327636 MS.gene46500:CDS 35.0%
TAAAGGGGAGAATGAAGATA+AGG - chr1.1:42326515-42326534 None:intergenic 35.0%
TGAGCCTTTAGATCTTGATT+AGG - chr1.1:42326621-42326640 None:intergenic 35.0%
TTCTTTACCACCATATGGAT+GGG + chr1.1:42326415-42326434 MS.gene46500:CDS 35.0%
TTGCTTGCTCTTTCCAAATA+AGG - chr1.1:42327494-42327513 None:intergenic 35.0%
! ACAGGATAAAGTTGTTGCTT+TGG + chr1.1:42327526-42327545 MS.gene46500:CDS 35.0%
! CGGAACAAAATGCTTTTCTT+CGG - chr1.1:42327222-42327241 None:intergenic 35.0%
! GGAACAAAATGCTTTTCTTC+GGG - chr1.1:42327221-42327240 None:intergenic 35.0%
! GTGCAAACTTCCTCTAAAAA+CGG - chr1.1:42327242-42327261 None:intergenic 35.0%
! TCACAAGATACCCTTGTTTA+TGG - chr1.1:42326974-42326993 None:intergenic 35.0%
!! CCTAACTTAATGATTCGGTA+GGG - chr1.1:42326594-42326613 None:intergenic 35.0%
!! GCATTTTGTTCCGTTTTTAG+AGG + chr1.1:42327229-42327248 MS.gene46500:CDS 35.0%
AATCCCGTTTGTTTCGACAA+TGG + chr1.1:42327297-42327316 MS.gene46500:CDS 40.0%
AATGGAGGATATAGAAGCTG+GGG + chr1.1:42326915-42326934 MS.gene46500:CDS 40.0%
ACTCAGCAAAAGTTCAATGG+AGG + chr1.1:42326900-42326919 MS.gene46500:CDS 40.0%
ATCCCGTTTGTTTCGACAAT+GGG + chr1.1:42327298-42327317 MS.gene46500:CDS 40.0%
CAATCTTAAGAGTTTGTGGG+AGG + chr1.1:42327397-42327416 MS.gene46500:CDS 40.0%
CAATGGAGGATATAGAAGCT+GGG + chr1.1:42326914-42326933 MS.gene46500:CDS 40.0%
CCCTACCGAATCATTAAGTT+AGG + chr1.1:42326591-42326610 MS.gene46500:intron 40.0%
GAAGAAGCTTAGGGATGAAT+TGG + chr1.1:42327559-42327578 MS.gene46500:CDS 40.0%
GACGCCCTAACTTAATGATT+CGG - chr1.1:42326599-42326618 None:intergenic 40.0%
GAGACTCAGCAAAAGTTCAA+TGG + chr1.1:42326897-42326916 MS.gene46500:intron 40.0%
GATGTTGAACCTGATGTTCT+TGG - chr1.1:42327125-42327144 None:intergenic 40.0%
GCGTCCTAATCAAGATCTAA+AGG + chr1.1:42326614-42326633 MS.gene46500:intron 40.0%
GGAGGAACTAGCAAAATCAT+CGG + chr1.1:42327415-42327434 MS.gene46500:CDS 40.0%
TCAACATCTAGTCCAGATGA+AGG + chr1.1:42327137-42327156 MS.gene46500:CDS 40.0%
TCAATGGAGGATATAGAAGC+TGG + chr1.1:42326913-42326932 MS.gene46500:CDS 40.0%
TTTCTATCCCATCCATATGG+TGG - chr1.1:42326425-42326444 None:intergenic 40.0%
! AAGCAGAGTTTGAAGTTGCA+AGG + chr1.1:42327591-42327610 MS.gene46500:CDS 40.0%
! GAGTTTGAAGTTGCAAGGAA+AGG + chr1.1:42327596-42327615 MS.gene46500:CDS 40.0%
! GCTGATTCTTTACCACCATA+TGG + chr1.1:42326410-42326429 MS.gene46500:CDS 40.0%
! GTGTTTCTATCCCATCCATA+TGG - chr1.1:42326428-42326447 None:intergenic 40.0%
! GTTAGTTCTCGTTAATCAGC+TGG + chr1.1:42326457-42326476 MS.gene46500:CDS 40.0%
!! CCCTAACTTAATGATTCGGT+AGG - chr1.1:42326595-42326614 None:intergenic 40.0%
!! TTTTAGAGGAAGTTTGCACG+TGG + chr1.1:42327243-42327262 MS.gene46500:CDS 40.0%
ACGCCCATTGTCGAAACAAA+CGG - chr1.1:42327304-42327323 None:intergenic 45.0%
AGATCCTTGTGCATCCACTA+AGG + chr1.1:42326351-42326370 MS.gene46500:CDS 45.0%
CCAGATGAAGGAGAGATTCA+AGG + chr1.1:42327149-42327168 MS.gene46500:CDS 45.0%
CCTTGAATCTCTCCTTCATC+TGG - chr1.1:42327152-42327171 None:intergenic 45.0%
! CATCGGAATTCGACTTGAGT+TGG + chr1.1:42327432-42327451 MS.gene46500:CDS 45.0%
CCTTGTGCATCCACTAAGGA+GGG + chr1.1:42326355-42326374 MS.gene46500:CDS 50.0%
CGCCCATTGTCGAAACAAAC+GGG - chr1.1:42327303-42327322 None:intergenic 50.0%
TCCTTGTGCATCCACTAAGG+AGG + chr1.1:42326354-42326373 MS.gene46500:CDS 50.0%
TGGTCAACATCCCTCCTTAG+TGG - chr1.1:42326368-42326387 None:intergenic 50.0%
! CCCTCCTTAGTGGATGCACA+AGG - chr1.1:42326358-42326377 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 42326323 42327688 42326323 ID=MS.gene46500
chr1.1 mRNA 42326323 42327688 42326323 ID=MS.gene46500.t1;Parent=MS.gene46500
chr1.1 exon 42326323 42326503 42326323 ID=MS.gene46500.t1.exon1;Parent=MS.gene46500.t1
chr1.1 CDS 42326323 42326503 42326323 ID=cds.MS.gene46500.t1;Parent=MS.gene46500.t1
chr1.1 exon 42326898 42327688 42326898 ID=MS.gene46500.t1.exon2;Parent=MS.gene46500.t1
chr1.1 CDS 42326898 42327688 42326898 ID=cds.MS.gene46500.t1;Parent=MS.gene46500.t1
Gene Sequence

>MS.gene46500

ATGTATATGTCTTCATCATGTGATAGGAAGATCCTTGTGCATCCACTAAGGAGGGATGTTGACCATTTTACGTTGTATTTAATGGTTGCTGATTCTTTACCACCATATGGATGGGATAGAAACACCTTCTTCAAGTTAGTTCTCGTTAATCAGCTGGATAAGAACAAGAACATTGTAAAAGGTTCTAACCCTTATCTTCATTCTCCCCTTTACTAGTTTTGTCATTTATAATAACAAATTTTAAACTAATTCAGTAATTTAGTAGATTCCCTACCGAATCATTAAGTTAGGGCGTCCTAATCAAGATCTAAAGGCTCATATTTGGTTTTCTCAAATATGAACAGTTTGATCTTAATAGGACGATACTGACTTGTTGATTGTGTTAATACGTAAATTACATAGAGAATTGAGACTATTTTGAGAGTTAATTTTAGACTTCTAGATGAAAGGGAATTGAGACTTCTAGATCTAAAATTTAGATTTCTAGATTCTATTTTGAGATATTTTCCTCTAATGATAATATTTTTTGTTAAATATGAAAGCTAATTCTTCTTATGCGTACTTATTATTTTTAGAGACTCAGCAAAAGTTCAATGGAGGATATAGAAGCTGGGGTGCATTTTTCCTAAATTTCAATAAATTCATTGACCATAAACAAGGGTATCTTGTGAGAGACACATGCATAATTGAAGCTCATATTTGTGTGTCTAACTTTGCACCAAAAATTCATGACATCAATTCCTTGAACCAAAATTCATCTCTAACAGATCAATCTTCAGATGAAAGGGAAACAATTAGTCCAAGAACATCAGGTTCAACATCTAGTCCAGATGAAGGAGAGATTCAAGGTACAGATTTAACATTAAGACAATTTATAGATTTGGATGGATTAAAACCCGAAGAAAAGCATTTTGTTCCGTTTTTAGAGGAAGTTTGCACGTGGCGTCCGTCTTTGATACAAAGTCAAATGAAAAAATCCCGTTTGTTTCGACAATGGGCGTTTATTTCATTAGGAAAAGTTCTGTATTTTCTTAAGACGAAGAAAGTGAAAGATATGAGTGAAGATGACATAAACAATCTTAAGAGTTTGTGGGAGGAACTAGCAAAATCATCGGAATTCGACTTGAGTTGGTTAGAGCCTTATGTTAAATCTGCTTTAGGTGTGAAACCTTATTTGGAAAGAGCAAGCAAATTGAAGAAATTACAGGATAAAGTTGTTGCTTTGGATATTAAAATGAAGAAGCTTAGGGATGAATTGGCTGCTTCACAAGCAGAGTTTGAAGTTGCAAGGAAAGGCTTATCAGAAATCAGAAATGGTTTCCAAGAAATGAATGTTAATGCTACAATAGGTTATGCTTTGTTTTAA

Protein sequence

>MS.gene46500.t1

MYMSSSCDRKILVHPLRRDVDHFTLYLMVADSLPPYGWDRNTFFKLVLVNQLDKNKNIVKETQQKFNGGYRSWGAFFLNFNKFIDHKQGYLVRDTCIIEAHICVSNFAPKIHDINSLNQNSSLTDQSSDERETISPRTSGSTSSPDEGEIQGTDLTLRQFIDLDGLKPEEKHFVPFLEEVCTWRPSLIQSQMKKSRLFRQWAFISLGKVLYFLKTKKVKDMSEDDINNLKSLWEELAKSSEFDLSWLEPYVKSALGVKPYLERASKLKKLQDKVVALDIKMKKLRDELAASQAEFEVARKGLSEIRNGFQEMNVNATIGYALF