Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55950.t1 | XP_003594683.1 | 98.1 | 361 | 7 | 0 | 1 | 361 | 1 | 361 | 2.00E-209 | 738 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55950.t1 | Q9FGY4 | 68.2 | 365 | 106 | 4 | 1 | 361 | 1 | 359 | 7.5e-144 | 511.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55950.t1 | B7FKY1 | 98.1 | 361 | 7 | 0 | 1 | 361 | 1 | 361 | 1.5e-209 | 738.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene55950 | 0.846638 | 1.92E-59 | -1.69E-46 |
| MS.gene049368 | MS.gene55950 | 0.807088 | 6.00E-50 | -1.69E-46 |
| MS.gene049419 | MS.gene55950 | -0.832362 | 9.87E-56 | -1.69E-46 |
| MS.gene049499 | MS.gene55950 | 0.80579 | 1.13E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene55950 | 0.830076 | 3.60E-55 | -1.69E-46 |
| MS.gene050414 | MS.gene55950 | 0.816897 | 4.36E-52 | -1.69E-46 |
| MS.gene050646 | MS.gene55950 | 0.837323 | 5.56E-57 | -1.69E-46 |
| MS.gene050654 | MS.gene55950 | 0.831654 | 1.48E-55 | -1.69E-46 |
| MS.gene050752 | MS.gene55950 | 0.803562 | 3.29E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene55950 | 0.815906 | 7.27E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene55950 | 0.808914 | 2.45E-50 | -1.69E-46 |
| MS.gene051043 | MS.gene55950 | 0.819183 | 1.33E-52 | -1.69E-46 |
| MS.gene051343 | MS.gene55950 | 0.807127 | 5.88E-50 | -1.69E-46 |
| MS.gene051902 | MS.gene55950 | 0.829133 | 6.11E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene55950 | 0.814364 | 1.60E-51 | -1.69E-46 |
| MS.gene052052 | MS.gene55950 | 0.80246 | 5.55E-49 | -1.69E-46 |
| MS.gene052072 | MS.gene55950 | 0.802553 | 5.31E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene55950 | 0.82036 | 7.15E-53 | -1.69E-46 |
| MS.gene052518 | MS.gene55950 | 0.804191 | 2.43E-49 | -1.69E-46 |
| MS.gene052932 | MS.gene55950 | 0.814205 | 1.73E-51 | -1.69E-46 |
| MS.gene053058 | MS.gene55950 | 0.818733 | 1.68E-52 | -1.69E-46 |
| MS.gene053298 | MS.gene55950 | 0.82345 | 1.38E-53 | -1.69E-46 |
| MS.gene053564 | MS.gene55950 | 0.807748 | 4.34E-50 | -1.69E-46 |
| MS.gene053879 | MS.gene55950 | 0.827147 | 1.84E-54 | -1.69E-46 |
| MS.gene053963 | MS.gene55950 | 0.806142 | 9.50E-50 | -1.69E-46 |
| MS.gene054222 | MS.gene55950 | 0.815137 | 1.08E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene55950 | 0.806398 | 8.39E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene55950 | 0.83103 | 2.10E-55 | -1.69E-46 |
| MS.gene05469 | MS.gene55950 | 0.819977 | 8.75E-53 | -1.69E-46 |
| MS.gene054892 | MS.gene55950 | 0.808332 | 3.26E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene55950 | 0.816151 | 6.41E-52 | -1.69E-46 |
| MS.gene054957 | MS.gene55950 | 0.810797 | 9.64E-51 | -1.69E-46 |
| MS.gene05517 | MS.gene55950 | 0.802852 | 4.61E-49 | -1.69E-46 |
| MS.gene055311 | MS.gene55950 | 0.802744 | 4.85E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene55950 | 0.830329 | 3.12E-55 | -1.69E-46 |
| MS.gene056181 | MS.gene55950 | 0.800796 | 1.22E-48 | -1.69E-46 |
| MS.gene056986 | MS.gene55950 | 0.817187 | 3.76E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene55950 | 0.808528 | 2.96E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene55950 | 0.809465 | 1.87E-50 | -1.69E-46 |
| MS.gene05781 | MS.gene55950 | 0.819771 | 9.75E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene55950 | 0.808107 | 3.64E-50 | -1.69E-46 |
| MS.gene058159 | MS.gene55950 | 0.82108 | 4.88E-53 | -1.69E-46 |
| MS.gene058279 | MS.gene55950 | 0.802352 | 5.84E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene55950 | 0.833965 | 3.94E-56 | -1.69E-46 |
| MS.gene05952 | MS.gene55950 | 0.801163 | 1.03E-48 | -1.69E-46 |
| MS.gene05985 | MS.gene55950 | -0.830121 | 3.51E-55 | -1.69E-46 |
| MS.gene060259 | MS.gene55950 | 0.810743 | 9.90E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene55950 | 0.801124 | 1.04E-48 | -1.69E-46 |
| MS.gene060932 | MS.gene55950 | 0.807123 | 5.89E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene55950 | 0.815048 | 1.13E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene55950 | 0.844706 | 6.40E-59 | -1.69E-46 |
| MS.gene060937 | MS.gene55950 | 0.860403 | 2.12E-63 | -1.69E-46 |
| MS.gene061100 | MS.gene55950 | 0.801244 | 9.87E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55950.t1 | MTR_2g033410 | 98.061 | 361 | 7 | 0 | 1 | 361 | 1 | 361 | 0.0 | 736 |
| MS.gene55950.t1 | MTR_4g133170 | 26.599 | 297 | 184 | 12 | 9 | 287 | 4 | 284 | 1.19e-16 | 80.9 |
| MS.gene55950.t1 | MTR_3g436150 | 28.571 | 322 | 178 | 15 | 8 | 295 | 31 | 334 | 2.39e-15 | 77.0 |
| MS.gene55950.t1 | MTR_3g464470 | 24.771 | 327 | 195 | 12 | 4 | 297 | 2 | 310 | 3.95e-15 | 76.3 |
| MS.gene55950.t1 | MTR_3g006570 | 24.026 | 308 | 217 | 7 | 2 | 300 | 14 | 313 | 5.32e-15 | 75.9 |
| MS.gene55950.t1 | MTR_7g407070 | 27.019 | 322 | 189 | 15 | 8 | 295 | 31 | 340 | 6.33e-15 | 75.9 |
| MS.gene55950.t1 | MTR_2g055970 | 24.497 | 298 | 187 | 11 | 9 | 286 | 211 | 490 | 2.18e-14 | 74.7 |
| MS.gene55950.t1 | MTR_3g030330 | 25.497 | 302 | 169 | 12 | 8 | 285 | 19 | 288 | 2.23e-14 | 73.9 |
| MS.gene55950.t1 | MTR_5g069760 | 25.464 | 377 | 213 | 17 | 6 | 338 | 2 | 354 | 2.26e-14 | 73.9 |
| MS.gene55950.t1 | MTR_7g070440 | 24.233 | 326 | 202 | 13 | 11 | 306 | 9 | 319 | 3.76e-14 | 73.2 |
| MS.gene55950.t1 | MTR_2g028110 | 26.667 | 300 | 188 | 11 | 9 | 287 | 4 | 292 | 4.78e-14 | 73.2 |
| MS.gene55950.t1 | MTR_3g023930 | 25.623 | 281 | 175 | 10 | 8 | 281 | 33 | 286 | 5.86e-14 | 72.8 |
| MS.gene55950.t1 | MTR_7g013590 | 26.453 | 344 | 221 | 13 | 12 | 335 | 39 | 370 | 1.18e-13 | 72.0 |
| MS.gene55950.t1 | MTR_1g094210 | 26.027 | 292 | 179 | 9 | 8 | 285 | 56 | 324 | 2.02e-13 | 71.2 |
| MS.gene55950.t1 | MTR_1g094210 | 26.027 | 292 | 179 | 9 | 8 | 285 | 56 | 324 | 2.58e-13 | 70.9 |
| MS.gene55950.t1 | MTR_2g021190 | 25.872 | 344 | 224 | 13 | 8 | 333 | 20 | 350 | 2.88e-13 | 70.9 |
| MS.gene55950.t1 | MTR_2g047200 | 23.747 | 379 | 205 | 15 | 8 | 330 | 19 | 369 | 4.09e-13 | 70.5 |
| MS.gene55950.t1 | MTR_3g010520 | 25.000 | 280 | 179 | 8 | 8 | 281 | 24 | 278 | 4.68e-13 | 70.1 |
| MS.gene55950.t1 | MTR_4g109280 | 27.397 | 292 | 171 | 13 | 10 | 283 | 9 | 277 | 5.15e-13 | 69.7 |
| MS.gene55950.t1 | MTR_3g030250 | 21.951 | 287 | 187 | 8 | 8 | 281 | 36 | 298 | 5.35e-13 | 69.7 |
| MS.gene55950.t1 | MTR_6g055210 | 24.631 | 406 | 241 | 17 | 10 | 359 | 23 | 419 | 7.72e-13 | 69.7 |
| MS.gene55950.t1 | MTR_2g047832 | 26.870 | 361 | 203 | 17 | 9 | 326 | 29 | 371 | 1.68e-12 | 68.6 |
| MS.gene55950.t1 | MTR_4g024890 | 24.691 | 324 | 186 | 11 | 7 | 293 | 8 | 310 | 2.54e-12 | 67.8 |
| MS.gene55950.t1 | MTR_3g085160 | 22.148 | 298 | 192 | 11 | 9 | 288 | 50 | 325 | 2.55e-12 | 67.8 |
| MS.gene55950.t1 | MTR_3g085160 | 22.148 | 298 | 192 | 11 | 9 | 288 | 119 | 394 | 3.38e-12 | 67.8 |
| MS.gene55950.t1 | MTR_3g464500 | 24.233 | 326 | 193 | 11 | 4 | 293 | 22 | 329 | 4.68e-12 | 67.0 |
| MS.gene55950.t1 | MTR_2g046920 | 26.912 | 353 | 209 | 17 | 4 | 326 | 13 | 346 | 5.09e-12 | 67.0 |
| MS.gene55950.t1 | MTR_4g134390 | 24.695 | 328 | 212 | 11 | 2 | 305 | 10 | 326 | 5.34e-12 | 67.0 |
| MS.gene55950.t1 | MTR_2g021150 | 26.689 | 296 | 183 | 11 | 10 | 285 | 15 | 296 | 5.51e-12 | 66.6 |
| MS.gene55950.t1 | MTR_2g046830 | 24.852 | 338 | 215 | 15 | 11 | 326 | 27 | 347 | 5.59e-12 | 67.0 |
| MS.gene55950.t1 | MTR_2g047892 | 23.869 | 398 | 238 | 17 | 8 | 360 | 21 | 398 | 7.22e-12 | 66.6 |
| MS.gene55950.t1 | MTR_7g060960 | 25.806 | 310 | 189 | 10 | 4 | 301 | 27 | 307 | 1.67e-11 | 65.5 |
| MS.gene55950.t1 | MTR_7g060940 | 25.269 | 372 | 183 | 17 | 3 | 321 | 16 | 345 | 1.77e-11 | 65.5 |
| MS.gene55950.t1 | MTR_5g096910 | 23.662 | 355 | 217 | 11 | 10 | 330 | 18 | 352 | 2.10e-11 | 65.1 |
| MS.gene55950.t1 | MTR_5g022090 | 22.297 | 296 | 180 | 7 | 9 | 285 | 34 | 298 | 2.10e-11 | 65.1 |
| MS.gene55950.t1 | MTR_7g017400 | 21.711 | 304 | 205 | 8 | 7 | 301 | 96 | 375 | 2.30e-11 | 65.1 |
| MS.gene55950.t1 | MTR_2g028130 | 22.678 | 366 | 246 | 12 | 9 | 341 | 5 | 366 | 2.61e-11 | 65.1 |
| MS.gene55950.t1 | MTR_2g021155 | 27.465 | 284 | 179 | 10 | 12 | 281 | 39 | 309 | 2.93e-11 | 65.5 |
| MS.gene55950.t1 | MTR_7g445890 | 23.929 | 397 | 228 | 17 | 8 | 359 | 15 | 382 | 3.46e-11 | 64.3 |
| MS.gene55950.t1 | MTR_4g116330 | 24.259 | 371 | 240 | 16 | 9 | 359 | 23 | 372 | 3.77e-11 | 64.3 |
| MS.gene55950.t1 | MTR_3g010590 | 23.183 | 289 | 179 | 9 | 8 | 281 | 38 | 298 | 4.92e-11 | 63.9 |
| MS.gene55950.t1 | MTR_3g084690 | 22.222 | 315 | 207 | 10 | 12 | 297 | 6 | 311 | 6.43e-11 | 63.5 |
| MS.gene55950.t1 | MTR_7g066850 | 23.229 | 353 | 213 | 11 | 8 | 321 | 22 | 355 | 6.48e-11 | 63.9 |
| MS.gene55950.t1 | MTR_2g031640 | 23.056 | 360 | 233 | 16 | 4 | 341 | 2 | 339 | 6.79e-11 | 63.5 |
| MS.gene55950.t1 | MTR_0064s0160 | 21.750 | 400 | 256 | 14 | 9 | 360 | 42 | 432 | 8.38e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55950.t1 | AT5G49610 | 68.219 | 365 | 106 | 4 | 1 | 361 | 1 | 359 | 0.0 | 509 |
| MS.gene55950.t1 | AT2G02030 | 25.249 | 301 | 175 | 7 | 9 | 270 | 39 | 328 | 1.13e-16 | 80.5 |
| MS.gene55950.t1 | AT1G33530 | 29.758 | 289 | 171 | 11 | 9 | 286 | 97 | 364 | 6.82e-16 | 79.0 |
| MS.gene55950.t1 | AT3G16210 | 26.984 | 252 | 144 | 12 | 10 | 251 | 6 | 227 | 6.57e-12 | 66.6 |
| MS.gene55950.t1 | AT1G12855 | 26.357 | 258 | 158 | 12 | 9 | 238 | 71 | 324 | 1.20e-11 | 66.2 |
Find 81 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACCATATGACCCAATTTA+TGG | 0.121920 | 2.3:-57149531 | MS.gene55950:CDS |
| TGATTTGTGTTGATAGTTTA+AGG | 0.134415 | 2.3:-57150034 | MS.gene55950:CDS |
| TTGATTTAAGTTGTGATATT+TGG | 0.191652 | 2.3:-57149566 | MS.gene55950:CDS |
| CTGTTTCCTGCTCCATAAAT+TGG | 0.196666 | 2.3:+57149519 | None:intergenic |
| CGGAAGCGTGGATGATTATC+TGG | 0.209263 | 2.3:-57149449 | MS.gene55950:CDS |
| CTGTTCTAGTATTCCGGATA+TGG | 0.232572 | 2.3:-57149937 | MS.gene55950:CDS |
| AATTATACAATGATGTTTCT+AGG | 0.255229 | 2.3:-57150115 | MS.gene55950:CDS |
| GCAAAACTGTGTGCAAATTA+TGG | 0.285845 | 2.3:-57150169 | MS.gene55950:CDS |
| AACACAAATCAAACTAGATT+TGG | 0.316362 | 2.3:+57150046 | None:intergenic |
| GGCAACTCATAAGCAGGTTT+TGG | 0.318987 | 2.3:-57149307 | MS.gene55950:CDS |
| TCAGAAGTTTAATGTTGTTC+TGG | 0.321567 | 2.3:-57149784 | MS.gene55950:CDS |
| ATCTGCATCACAACCTCATC+TGG | 0.340038 | 2.3:+57150227 | None:intergenic |
| GTTGTGCTGTTCTAGTATTC+CGG | 0.341330 | 2.3:-57149943 | MS.gene55950:CDS |
| GGATGGGAAGTTTATATGTT+TGG | 0.353754 | 2.3:-57149730 | MS.gene55950:CDS |
| ACAGGCCTCTCCCTACTCTT+AGG | 0.361424 | 2.3:+57149867 | None:intergenic |
| AACCTAAACTCACGCGTAAC+CGG | 0.364514 | 2.3:+57149894 | None:intergenic |
| ATCCGGTTACGCGTGAGTTT+AGG | 0.364961 | 2.3:-57149896 | MS.gene55950:CDS |
| CAATTTATGGAGCAGGAAAC+AGG | 0.386807 | 2.3:-57149518 | MS.gene55950:CDS |
| TCTGTCTGTGATTCAGATTT+CGG | 0.387877 | 2.3:-57149469 | MS.gene55950:CDS |
| ATAGCTCAACACTTCCGTTA+TGG | 0.391598 | 2.3:-57149236 | MS.gene55950:CDS |
| GCTCCATAAATTGGGTCATA+TGG | 0.396146 | 2.3:+57149528 | None:intergenic |
| GAAATCTCAACAAGAATCAT+AGG | 0.403273 | 2.3:+57150086 | None:intergenic |
| TGTTTCCTGCTCCATAAATT+GGG | 0.405077 | 2.3:+57149520 | None:intergenic |
| ACTTACCTGTTGGAACTTGA+TGG | 0.405989 | 2.3:-57149495 | MS.gene55950:CDS |
| TATATGTTTGGTGTTTGATT+CGG | 0.406217 | 2.3:-57149718 | MS.gene55950:CDS |
| ATCAGCGCAGGAGCCAAGTT+TGG | 0.412670 | 2.3:-57149281 | MS.gene55950:CDS |
| TGTTCTAGTATTCCGGATAT+GGG | 0.418304 | 2.3:-57149936 | MS.gene55950:CDS |
| GATTTGTGTTGATAGTTTAA+GGG | 0.421401 | 2.3:-57150033 | MS.gene55950:CDS |
| AAGGTTCGCGCGTCGTGTAA+TGG | 0.433866 | 2.3:-57149969 | MS.gene55950:CDS |
| ATTATGGTATAGGTTGACTT+TGG | 0.440149 | 2.3:-57150153 | MS.gene55950:CDS |
| AATACATTTCTTTCCAAACT+TGG | 0.442610 | 2.3:+57149268 | None:intergenic |
| TTGTTCTGGCTGGTAGTCAT+AGG | 0.443055 | 2.3:-57149770 | MS.gene55950:CDS |
| GTCATAGGACGTTTGGTCAT+AGG | 0.447514 | 2.3:-57149755 | MS.gene55950:CDS |
| CTGTGTGCAAATTATGGTAT+AGG | 0.447965 | 2.3:-57150163 | MS.gene55950:CDS |
| GGTTGGTTTAGCTTGTGATT+CGG | 0.452708 | 2.3:-57149811 | MS.gene55950:CDS |
| AAGTTTAATGTTGTTCTGGC+TGG | 0.464144 | 2.3:-57149780 | MS.gene55950:CDS |
| TCCCGATGGTGAAGCTACTT+TGG | 0.468012 | 2.3:-57149832 | MS.gene55950:CDS |
| TGAATGGTGCATTGTGGATA+AGG | 0.468222 | 2.3:-57149403 | MS.gene55950:CDS |
| GATGGTGAAGCTACTTTGGT+TGG | 0.470218 | 2.3:-57149828 | MS.gene55950:CDS |
| GGTTTACTATGTTTGCAATC+CGG | 0.471771 | 2.3:-57149913 | MS.gene55950:CDS |
| TATTCACTTGTCTGACTGAT+CGG | 0.474149 | 2.3:+57149339 | None:intergenic |
| GCTGGTAGTCATAGGACGTT+TGG | 0.476737 | 2.3:-57149762 | MS.gene55950:CDS |
| TAGGTTGCTTCCTAAGAGTA+GGG | 0.490597 | 2.3:-57149877 | MS.gene55950:CDS |
| ACCAAAGTAGCTTCACCATC+GGG | 0.500934 | 2.3:+57149831 | None:intergenic |
| TTTCTTGGCAACTCATAAGC+AGG | 0.501775 | 2.3:-57149313 | MS.gene55950:CDS |
| TGACTGATCGGGAAAATGCC+TGG | 0.505550 | 2.3:+57149351 | None:intergenic |
| TTGATTCGGAGTTGAATAAG+TGG | 0.507037 | 2.3:-57149704 | MS.gene55950:CDS |
| ATCATGGATAAATCAGGAGA+TGG | 0.507056 | 2.3:-57150257 | MS.gene55950:CDS |
| ATTTAAGTTGTGATATTTGG+AGG | 0.507064 | 2.3:-57149563 | MS.gene55950:CDS |
| GACAACCATCAAGTTCCAAC+AGG | 0.507357 | 2.3:+57149490 | None:intergenic |
| TTAGGTTGCTTCCTAAGAGT+AGG | 0.508617 | 2.3:-57149878 | MS.gene55950:CDS |
| TCATGGATAAATCAGGAGAT+GGG | 0.514329 | 2.3:-57150256 | MS.gene55950:CDS |
| GTTCTAGTATTCCGGATATG+GGG | 0.517089 | 2.3:-57149935 | MS.gene55950:CDS |
| TATGACCCAATTTATGGAGC+AGG | 0.517303 | 2.3:-57149525 | MS.gene55950:CDS |
| ATTCACTTGTCTGACTGATC+GGG | 0.518671 | 2.3:+57149340 | None:intergenic |
| GGAAGCGTGGATGATTATCT+GGG | 0.543098 | 2.3:-57149448 | MS.gene55950:CDS |
| TGTATCAGAGGAATGGTGCC+AGG | 0.547546 | 2.3:-57149369 | MS.gene55950:CDS |
| TCGGGATAAAACCGAGTCAC+AGG | 0.552202 | 2.3:+57149849 | None:intergenic |
| TTCTAGTATTCCGGATATGG+GGG | 0.554158 | 2.3:-57149934 | MS.gene55950:CDS |
| ACGTTTGGTCATAGGCCGGA+TGG | 0.559674 | 2.3:-57149747 | MS.gene55950:CDS |
| CGTTTGGTCATAGGCCGGAT+GGG | 0.563829 | 2.3:-57149746 | MS.gene55950:CDS |
| ATTTGTGTTGATAGTTTAAG+GGG | 0.568974 | 2.3:-57150032 | MS.gene55950:CDS |
| AAACATATAAACTTCCCATC+CGG | 0.573486 | 2.3:+57149732 | None:intergenic |
| AACCAAAGTAGCTTCACCAT+CGG | 0.576018 | 2.3:+57149830 | None:intergenic |
| TTGTGATGCAGATTCTTGCA+AGG | 0.583840 | 2.3:-57150217 | MS.gene55950:CDS |
| TGATTCAGATTTCGGAAGCG+TGG | 0.586875 | 2.3:-57149461 | MS.gene55950:CDS |
| ATGTTAATAATGCTTTGCAT+TGG | 0.589977 | 2.3:-57149620 | MS.gene55950:CDS |
| CTTCTGAAAAGCGATTTCAC+AGG | 0.596408 | 2.3:+57150191 | None:intergenic |
| TGCTTCCTAAGAGTAGGGAG+AGG | 0.597743 | 2.3:-57149872 | MS.gene55950:CDS |
| GTAGGGAGAGGCCTGTGACT+CGG | 0.598142 | 2.3:-57149860 | MS.gene55950:CDS |
| AAGATTACTTGATAGATGAA+TGG | 0.607504 | 2.3:-57149419 | MS.gene55950:CDS |
| TAGGACGTTTGGTCATAGGC+CGG | 0.610500 | 2.3:-57149751 | MS.gene55950:CDS |
| AGGAAACAGGACTTACCTGT+TGG | 0.624064 | 2.3:-57149505 | MS.gene55950:CDS |
| ATTCGGAGTTGAATAAGTGG+AGG | 0.625638 | 2.3:-57149701 | MS.gene55950:CDS |
| CATGGATAAATCAGGAGATG+GGG | 0.626281 | 2.3:-57150255 | MS.gene55950:CDS |
| GTGAGTCTGAGGTGTATCAG+AGG | 0.650428 | 2.3:-57149381 | MS.gene55950:CDS |
| TCTGAGGTGTATCAGAGGAA+TGG | 0.669764 | 2.3:-57149376 | MS.gene55950:CDS |
| GCATGCGCTGAAAACCATAA+CGG | 0.687822 | 2.3:+57149222 | None:intergenic |
| ACAATGTTCTCATGTAACTG+AGG | 0.694688 | 2.3:-57149189 | None:intergenic |
| TTGTGGATAAGGTGAGTCTG+AGG | 0.695028 | 2.3:-57149392 | MS.gene55950:CDS |
| GATAGATGAATGGTGCATTG+TGG | 0.697733 | 2.3:-57149409 | MS.gene55950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATACAATGATGTTTCT+AGG | - | chr2.3:57149330-57149349 | MS.gene55950:CDS | 20.0% |
| !!! | TTGATTTAAGTTGTGATATT+TGG | - | chr2.3:57149879-57149898 | MS.gene55950:CDS | 20.0% |
| !!! | TTTTTTGAATGATAGAGTTA+AGG | - | chr2.3:57149457-57149476 | MS.gene55950:CDS | 20.0% |
| ! | AACACAAATCAAACTAGATT+TGG | + | chr2.3:57149402-57149421 | None:intergenic | 25.0% |
| ! | AAGATTACTTGATAGATGAA+TGG | - | chr2.3:57150026-57150045 | MS.gene55950:CDS | 25.0% |
| ! | AATACATTTCTTTCCAAACT+TGG | + | chr2.3:57150180-57150199 | None:intergenic | 25.0% |
| ! | ATGTTAATAATGCTTTGCAT+TGG | - | chr2.3:57149825-57149844 | MS.gene55950:CDS | 25.0% |
| !!! | ATTTAAGTTGTGATATTTGG+AGG | - | chr2.3:57149882-57149901 | MS.gene55950:CDS | 25.0% |
| !!! | ATTTGTGTTGATAGTTTAAG+GGG | - | chr2.3:57149413-57149432 | MS.gene55950:CDS | 25.0% |
| !!! | GATTTGTGTTGATAGTTTAA+GGG | - | chr2.3:57149412-57149431 | MS.gene55950:CDS | 25.0% |
| !!! | TATATGTTTGGTGTTTGATT+CGG | - | chr2.3:57149727-57149746 | MS.gene55950:CDS | 25.0% |
| !!! | TGATTTGTGTTGATAGTTTA+AGG | - | chr2.3:57149411-57149430 | MS.gene55950:CDS | 25.0% |
| AAACATATAAACTTCCCATC+CGG | + | chr2.3:57149716-57149735 | None:intergenic | 30.0% | |
| GAAATCTCAACAAGAATCAT+AGG | + | chr2.3:57149362-57149381 | None:intergenic | 30.0% | |
| TCAGAAGTTTAATGTTGTTC+TGG | - | chr2.3:57149661-57149680 | MS.gene55950:CDS | 30.0% | |
| TTACCATATGACCCAATTTA+TGG | - | chr2.3:57149914-57149933 | MS.gene55950:CDS | 30.0% | |
| ! | ATTATGGTATAGGTTGACTT+TGG | - | chr2.3:57149292-57149311 | MS.gene55950:CDS | 30.0% |
| ! | GACAAGTGAATATGTTTTCT+TGG | - | chr2.3:57150117-57150136 | MS.gene55950:CDS | 30.0% |
| AAGTTTAATGTTGTTCTGGC+TGG | - | chr2.3:57149665-57149684 | MS.gene55950:CDS | 35.0% | |
| CTGTGTGCAAATTATGGTAT+AGG | - | chr2.3:57149282-57149301 | MS.gene55950:CDS | 35.0% | |
| GCAAAACTGTGTGCAAATTA+TGG | - | chr2.3:57149276-57149295 | MS.gene55950:CDS | 35.0% | |
| GGATGGGAAGTTTATATGTT+TGG | - | chr2.3:57149715-57149734 | MS.gene55950:CDS | 35.0% | |
| GGTTTACTATGTTTGCAATC+CGG | - | chr2.3:57149532-57149551 | MS.gene55950:CDS | 35.0% | |
| TATTCACTTGTCTGACTGAT+CGG | + | chr2.3:57150109-57150128 | None:intergenic | 35.0% | |
| TCTGTCTGTGATTCAGATTT+CGG | - | chr2.3:57149976-57149995 | MS.gene55950:CDS | 35.0% | |
| TGTTCTAGTATTCCGGATAT+GGG | - | chr2.3:57149509-57149528 | MS.gene55950:CDS | 35.0% | |
| TGTTTCCTGCTCCATAAATT+GGG | + | chr2.3:57149928-57149947 | None:intergenic | 35.0% | |
| !! | TTGATTCGGAGTTGAATAAG+TGG | - | chr2.3:57149741-57149760 | MS.gene55950:CDS | 35.0% |
| AACCAAAGTAGCTTCACCAT+CGG | + | chr2.3:57149618-57149637 | None:intergenic | 40.0% | |
| ACATAGTAAACCCCCATATC+CGG | + | chr2.3:57149524-57149543 | None:intergenic | 40.0% | |
| ACTTACCTGTTGGAACTTGA+TGG | - | chr2.3:57149950-57149969 | MS.gene55950:CDS | 40.0% | |
| ATAGCTCAACACTTCCGTTA+TGG | - | chr2.3:57150209-57150228 | MS.gene55950:CDS | 40.0% | |
| ATTCACTTGTCTGACTGATC+GGG | + | chr2.3:57150108-57150127 | None:intergenic | 40.0% | |
| ATTCGGAGTTGAATAAGTGG+AGG | - | chr2.3:57149744-57149763 | MS.gene55950:CDS | 40.0% | |
| CAATTTATGGAGCAGGAAAC+AGG | - | chr2.3:57149927-57149946 | MS.gene55950:CDS | 40.0% | |
| CTGTTCTAGTATTCCGGATA+TGG | - | chr2.3:57149508-57149527 | MS.gene55950:CDS | 40.0% | |
| CTGTTTCCTGCTCCATAAAT+TGG | + | chr2.3:57149929-57149948 | None:intergenic | 40.0% | |
| GATAGATGAATGGTGCATTG+TGG | - | chr2.3:57150036-57150055 | MS.gene55950:CDS | 40.0% | |
| GCTCCATAAATTGGGTCATA+TGG | + | chr2.3:57149920-57149939 | None:intergenic | 40.0% | |
| GTTCTAGTATTCCGGATATG+GGG | - | chr2.3:57149510-57149529 | MS.gene55950:CDS | 40.0% | |
| TAGGTTGCTTCCTAAGAGTA+GGG | - | chr2.3:57149568-57149587 | MS.gene55950:CDS | 40.0% | |
| TATGACCCAATTTATGGAGC+AGG | - | chr2.3:57149920-57149939 | MS.gene55950:CDS | 40.0% | |
| TGAATGGTGCATTGTGGATA+AGG | - | chr2.3:57150042-57150061 | MS.gene55950:CDS | 40.0% | |
| TTCTAGTATTCCGGATATGG+GGG | - | chr2.3:57149511-57149530 | MS.gene55950:CDS | 40.0% | |
| TTGTGATGCAGATTCTTGCA+AGG | - | chr2.3:57149228-57149247 | MS.gene55950:CDS | 40.0% | |
| TTTCTTGGCAACTCATAAGC+AGG | - | chr2.3:57150132-57150151 | MS.gene55950:CDS | 40.0% | |
| ! | CTTCTGAAAAGCGATTTCAC+AGG | + | chr2.3:57149257-57149276 | None:intergenic | 40.0% |
| ! | GTTGTGCTGTTCTAGTATTC+CGG | - | chr2.3:57149502-57149521 | MS.gene55950:CDS | 40.0% |
| ! | TTAGGTTGCTTCCTAAGAGT+AGG | - | chr2.3:57149567-57149586 | MS.gene55950:CDS | 40.0% |
| !! | GGTTGGTTTAGCTTGTGATT+CGG | - | chr2.3:57149634-57149653 | MS.gene55950:CDS | 40.0% |
| AACCTAAACTCACGCGTAAC+CGG | + | chr2.3:57149554-57149573 | None:intergenic | 45.0% | |
| ACCAAAGTAGCTTCACCATC+GGG | + | chr2.3:57149617-57149636 | None:intergenic | 45.0% | |
| AGGAAACAGGACTTACCTGT+TGG | - | chr2.3:57149940-57149959 | MS.gene55950:CDS | 45.0% | |
| ATCTGCATCACAACCTCATC+TGG | + | chr2.3:57149221-57149240 | None:intergenic | 45.0% | |
| GACAACCATCAAGTTCCAAC+AGG | + | chr2.3:57149958-57149977 | None:intergenic | 45.0% | |
| GCATGCGCTGAAAACCATAA+CGG | + | chr2.3:57150226-57150245 | None:intergenic | 45.0% | |
| GTCATAGGACGTTTGGTCAT+AGG | - | chr2.3:57149690-57149709 | MS.gene55950:CDS | 45.0% | |
| TGATTCAGATTTCGGAAGCG+TGG | - | chr2.3:57149984-57150003 | MS.gene55950:CDS | 45.0% | |
| TTGTGGATAAGGTGAGTCTG+AGG | - | chr2.3:57150053-57150072 | MS.gene55950:CDS | 45.0% | |
| ! | AGATGGGGTTTTTCCAGATG+AGG | - | chr2.3:57149205-57149224 | MS.gene55950:CDS | 45.0% |
| ! | GATGGTGAAGCTACTTTGGT+TGG | - | chr2.3:57149617-57149636 | MS.gene55950:CDS | 45.0% |
| ! | GGAAGCGTGGATGATTATCT+GGG | - | chr2.3:57149997-57150016 | MS.gene55950:CDS | 45.0% |
| ! | GGCAACTCATAAGCAGGTTT+TGG | - | chr2.3:57150138-57150157 | MS.gene55950:CDS | 45.0% |
| ! | TCTGAGGTGTATCAGAGGAA+TGG | - | chr2.3:57150069-57150088 | MS.gene55950:CDS | 45.0% |
| ! | TTGTTCTGGCTGGTAGTCAT+AGG | - | chr2.3:57149675-57149694 | MS.gene55950:CDS | 45.0% |
| ATCCGGTTACGCGTGAGTTT+AGG | - | chr2.3:57149549-57149568 | MS.gene55950:CDS | 50.0% | |
| GCTGGTAGTCATAGGACGTT+TGG | - | chr2.3:57149683-57149702 | MS.gene55950:CDS | 50.0% | |
| TAGGACGTTTGGTCATAGGC+CGG | - | chr2.3:57149694-57149713 | MS.gene55950:CDS | 50.0% | |
| TCCCGATGGTGAAGCTACTT+TGG | - | chr2.3:57149613-57149632 | MS.gene55950:CDS | 50.0% | |
| TCGGGATAAAACCGAGTCAC+AGG | + | chr2.3:57149599-57149618 | None:intergenic | 50.0% | |
| TGACTGATCGGGAAAATGCC+TGG | + | chr2.3:57150097-57150116 | None:intergenic | 50.0% | |
| TGCTTCCTAAGAGTAGGGAG+AGG | - | chr2.3:57149573-57149592 | MS.gene55950:CDS | 50.0% | |
| TGTATCAGAGGAATGGTGCC+AGG | - | chr2.3:57150076-57150095 | MS.gene55950:CDS | 50.0% | |
| ! | CGGAAGCGTGGATGATTATC+TGG | - | chr2.3:57149996-57150015 | MS.gene55950:CDS | 50.0% |
| ! | GTGAGTCTGAGGTGTATCAG+AGG | - | chr2.3:57150064-57150083 | MS.gene55950:CDS | 50.0% |
| !! | AGGTTTTGGTGTATCAGCGC+AGG | - | chr2.3:57150152-57150171 | MS.gene55950:CDS | 50.0% |
| !!! | GTGACTCGGTTTTATCCCGA+TGG | - | chr2.3:57149599-57149618 | MS.gene55950:CDS | 50.0% |
| AAGGTTCGCGCGTCGTGTAA+TGG | - | chr2.3:57149476-57149495 | MS.gene55950:CDS | 55.0% | |
| ACAGGCCTCTCCCTACTCTT+AGG | + | chr2.3:57149581-57149600 | None:intergenic | 55.0% | |
| ACGTTTGGTCATAGGCCGGA+TGG | - | chr2.3:57149698-57149717 | MS.gene55950:CDS | 55.0% | |
| ATCAGCGCAGGAGCCAAGTT+TGG | - | chr2.3:57150164-57150183 | MS.gene55950:CDS | 55.0% | |
| CGTTTGGTCATAGGCCGGAT+GGG | - | chr2.3:57149699-57149718 | MS.gene55950:CDS | 55.0% | |
| GTAGGGAGAGGCCTGTGACT+CGG | - | chr2.3:57149585-57149604 | MS.gene55950:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 57149191 | 57150276 | 57149191 | ID=MS.gene55950 |
| chr2.3 | mRNA | 57149191 | 57150276 | 57149191 | ID=MS.gene55950.t1;Parent=MS.gene55950 |
| chr2.3 | exon | 57149191 | 57150276 | 57149191 | ID=MS.gene55950.t1.exon1;Parent=MS.gene55950.t1 |
| chr2.3 | CDS | 57149191 | 57150276 | 57149191 | ID=cds.MS.gene55950.t1;Parent=MS.gene55950.t1 |
| Gene Sequence |
| Protein sequence |
